Moc02g13170 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g13170
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionMethionine--tRNA ligase
Locationchr2: 9648705 .. 9651776 (-)
RNA-Seq ExpressionMoc02g13170
SyntenyMoc02g13170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTGACTATATGGGATTATTCTACTTGTTTGAAATTTACTACTGCTCTTTTGTGTGTGCAGCCACCTTCTATTTGTGTTTGTCTGTGAAATTTGTCTGTGAAATTTATAAACAGAAGTATAAGCACACACAAACACACATCATCACACACATATATATATCGTGAATGTCTAAACTTGATTATGCACACCTTGACTAATTACATGAGACATGAGACAACTACGAAATAATAGACTAGGGACAGGGGAAGAGAGAACCCCCTCCCAAGAGAGCTAATTACATGAATGCCTTCCAATTATGAAGGATCACTGTGAAAAGGGTAATTACAAAAGAGTTTGCTTTTAGTATAAAATTGAACGTTGCTAAAAAGAGAGGCAAACTTAGCTTCCCTCTCCTCAAAAAGTCTTGATTTCTCTCCAGCCAGCATCTCCATTGTAGAATGACTTGTAAGAGCGTTAATACAAAACTCCATGCACATATATCATAAATTATATTATTCAAGGTCTACCATTACCTATGACCTAAAGTAAACGTGAAAGTCTTGTAATGGTTCATCATATCTCTTTGATATTTTTTGATATTGTATTTTATTTAATCATTTTTCTGACAACAGAATGTCAGTTCTATTGTCTGTTACAATTTTTTCCTTCTTGCCGATCGTGAACCATTCTTTGATTTATTCAGCACCATATCTGCCAAGGTGAAAAGTTGGATTAGAAGTGGCCTAGAAGACTTCTCTATCTCCAGGGCTTCGGTTGAATGGGGTATTCCAGTTCCCAATGATCAAAAGCAAATAATTTATGTTTGGTTTGATGCATTGTTAGGGGTAATCTCTCTCTTATCTTTTCATTTTCATTTTTGTTTTTAAAGAATTCTTCATTACTACAAACATATGGTATATGTGAACTTGCAACAATTCTCCACCGTTTTGTTTAGGAAACCGAAATGGAAAGTATAGTCAGTGCCTTCAATCTTAGTCATATGAAAATTCCATAATGTAGTTATATATCAGCGGTATCAGAGAACTGTAAGCAACCAAATTTACAAAGGGCTTCCGCTTCAGGTTGGCCTGCCTCTTTACACTTAATTGGTGTTTTTCAATGATATTTTAGGGCAATTTTCCCGATTGTACATATTTCATCTAGCCCCGGCTGGAAAATATTTTCTTTTTCTCCACCTGATTTTTAGAATTTGGTCTTTGCTACATTTTTTTTTTAATATAACAAGAATTGAAATTATTCATGAGCTCCCATTTCTGTGTTATTTTGAATTCCGGAGGTTATGAGACTTTCCCTTTTTCCCATACTGAATTCAAGCTCCTCAGTCTGTAGGATGCAGATTTATTGTTTTTACTGTTCTGTTTGTAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCTGAACTAAAACTTCCAAAGATGGTTTTCGATCACGGGTTTCTGACAATGGTGTGTTTGGGTTCCTAAAGTTTCTATTAGACAAAGGAATTTAGTTGGCGATGGTAATGGGTGAAGCAGAAGAAAGTTAATATTTACTATTTAGGAAGTCTATAAAGGTGTAACCAGTCTCCATTGCCTTTATATATAATATGAATATTGTTGCACCTTGTGAATTCAAGGGTCTTCGTTGTCTTGTGAATCTTATCTCTTGGAGTTGGTCAATATTCAATGTCCCTCATAATCAAATATTTCCAACCATTTGGATGCTGCCAGGATTCATGAATTCTTGCATTGTGTAGAATTGGTTATTTTTGTTGGAATAGTGAGCAATAGGTAGATTTATAACTTGTTATATTTTGTAATAATGGAGAAAACTTCATTATTGTCTACTTTTTGGTGAATTTATTCTAATTCGAGAATTAAGCTATTATAATATGCTTCTTATCAATGCTGTTGCAAAGATTTTATGGTTCAAGCTCTTAATGTATTTGATGTATTTTGGTTACTAGCGATGTCAGTTATGGGCAAAGCCGAGTATTTATTTTAAAGAGAAGCTTGAAGACGTCCAGTTATGTGCGAATTTTTGAGTTTCTAGTTTCTCAAGCTTCTGGTGTCTAAGTAGTGGAACCATTCTTTTCATATTTCTTAGGGCCGTTTGATAACTATTTTGTTTTGGTTTTTAATTTTTGAAAATTATGCTGGTTTTATCAATATGTTTTTCACCTTTCTAAAGAATAACATTTGACTCGGTTAAATTTCAAAAACAAAAAAAAGTTTTTAAAAATTACTATTTTTAGTTTCAAACGTGATTTTGGAAACAAAACGTTTTTAGAAAAAGATAACAAAACAAAGAAATGCATAGGTGGAAGTAATGTTAATAAACTTAATTTTCAAAACTGAAAAGCCAAAACGAAAACCAAAATGATTACCGGTCCTTAGTTTTCCAGCTCTCCTGTAGAGAAAGTGTGATATTTCCTATAAATTGAATCCCTGTGATCTCTCCCTAACTTCTTTCTGGTTCATGGTTCGAGTGGTAAAGAGTGAGTAATAACAACTGAATATTTTATTACTAGAACTTAGATTCAGAATCCACATTAGATATATGAATTTGACATCATCGTGATCTTGTTTGTAAACATTTATTTCTGGAGTTCTTGTAGTCCTGGCCTTCTTTTGTCAATACAAACAATTGTTGGTCAAGTTTCGAAACCTCTCATAACTAATTTTGGTTCATGCATCAACTCTTTTTGTTTGAACTTTATAGGATGGAACGAGGATGGGCAAGTCATTAGGCAATACCCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTGGGAAGATGGAATTTGGTAGCGATGGAGACTATGCCAAGGATCGTTTCATCAATATTGTCAACGCACATCTAGCAAATACATTAGGTATTGATAAACCATAGTGCTGGTTCCTTTTGAATGGAAAAGGAAAACAACAGCCACTCTTACTTGAGATTTCTGTTTGACAACATAATTCTTGACATTCGCTTGCAGGAAATCTTCTTAACCGAACACTCAGACTCCTTATGAAGACTGCCAAACAACTTTGGTAG

mRNA sequence

ATGAGCACCATATCTGCCAAGGTGAAAAGTTGGATTAGAAGTGGCCTAGAAGACTTCTCTATCTCCAGGGCTTCGGTTGAATGGGGTATTCCAGTTCCCAATGATCAAAAGCAAATAATTTATGTTTGGTTTGATGCATTGTTAGGGGATGGAACGAGGATGGGCAAGTCATTAGGCAATACCCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTGGGAAGATGGAATTTGGTAGCGATGGAGACTATGCCAAGGATCGTTTCATCAATATTGTCAACGCACATCTAGCAAATACATTAGGAAATCTTCTTAACCGAACACTCAGACTCCTTATGAAGACTGCCAAACAACTTTGGTAG

Coding sequence (CDS)

ATGAGCACCATATCTGCCAAGGTGAAAAGTTGGATTAGAAGTGGCCTAGAAGACTTCTCTATCTCCAGGGCTTCGGTTGAATGGGGTATTCCAGTTCCCAATGATCAAAAGCAAATAATTTATGTTTGGTTTGATGCATTGTTAGGGGATGGAACGAGGATGGGCAAGTCATTAGGCAATACCCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTGGGAAGATGGAATTTGGTAGCGATGGAGACTATGCCAAGGATCGTTTCATCAATATTGTCAACGCACATCTAGCAAATACATTAGGAAATCTTCTTAACCGAACACTCAGACTCCTTATGAAGACTGCCAAACAACTTTGGTAG

Protein sequence

MSTISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW
Homology
[Close]
BLAST of Moc02g13170 vs. NCBI nr
Match: XP_022149556.1 (methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Momordica charantia])

HSP 1 Score: 260.0 bits (663), Expect = 1.1e-65
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0 

Query: 2   STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNT 61
           STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNT
Sbjct: 36  STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNT 95

Query: 62  LEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLM 121
           LEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLM
Sbjct: 96  LEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLM 155

Query: 122 KTAKQLW 129
           KTAKQLW
Sbjct: 156 KTAKQLW 162

[Close]
BLAST of Moc02g13170 vs. NCBI nr
Match: XP_022149555.1 (methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Momordica charantia])

HSP 1 Score: 243.0 bits (619), Expect = 1.4e-60
Identity = 127/160 (79.38%), Postives = 127/160 (79.38%), Query Frame = 0 

Query: 2   STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG------------ 61
           STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG            
Sbjct: 36  STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDILRFHAVYWPA 95

Query: 62  ---------------------DGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGS 121
                                DGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGS
Sbjct: 96  MLMSAELKLPKMVFDHGFLTMDGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGS 155

Query: 122 DGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW 129
           DGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW
Sbjct: 156 DGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW 195

[Close]
BLAST of Moc02g13170 vs. NCBI nr
Match: XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 187.6 bits (475), Expect = 6.8e-44
Identity = 103/183 (56.28%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWIRSGL+DFSISRASVEWGIPVPNDQKQ IYVWFDALLG                 
Sbjct: 263 EVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQQNLQRA 322

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 323 VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTL 382

[Close]
BLAST of Moc02g13170 vs. NCBI nr
Match: XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])

HSP 1 Score: 185.3 bits (469), Expect = 3.4e-43
Identity = 101/183 (55.19%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWIRSGL+DFSISRASVEWGIPVPND+KQ IYVWFDALLG                 
Sbjct: 258 EVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLGYISALSENGEQPNLQEV 317

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 318 VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTL 377

[Close]
BLAST of Moc02g13170 vs. NCBI nr
Match: KAA0066020.1 (methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 184.9 bits (468), Expect = 4.4e-43
Identity = 101/183 (55.19%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWIRSGL+DFSISRASVEWGIPVPND+KQ IYVWFDALLG                 
Sbjct: 258 EVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWFDALLGYISALSENGEQPNLHKV 317

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 318 VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTL 377

[Close]
BLAST of Moc02g13170 vs. ExPASy Swiss-Prot
Match: Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 4.3e-41
Identity = 93/183 (50.82%), Postives = 106/183 (57.92%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWI+SGL DFSISRA V+WGIPVP+D KQ IYVWFDALLG                 
Sbjct: 256 EVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFDALLGYISALTEDNKQQNLETA 315

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 316 VSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMVFGHGFLTKDGMKMGKSLGNTL 375

[Close]
BLAST of Moc02g13170 vs. ExPASy Swiss-Prot
Match: Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.4e-30
Identity = 72/180 (40.00%), Postives = 96/180 (53.33%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V +++  GL+DFSISR +++WG PVPND K  +YVWFDALL                  
Sbjct: 197 EVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLDPEDEPTLENA 256

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 123
                                                          DG +MGKSLGNT+
Sbjct: 257 LGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQKMGKSLGNTV 316

[Close]
BLAST of Moc02g13170 vs. ExPASy Swiss-Prot
Match: P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 5.8e-30
Identity = 72/180 (40.00%), Postives = 95/180 (52.78%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V S+I  GL+DFSISR ++ WG PVP D +Q +YVWFDALLG                 
Sbjct: 191 EVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDEPTLANA 250

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 123
                                                          DG +MGKSLGNTL
Sbjct: 251 LKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGKSLGNTL 310

[Close]
BLAST of Moc02g13170 vs. ExPASy Swiss-Prot
Match: Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-29
Identity = 68/180 (37.78%), Postives = 94/180 (52.22%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V +++  GL+DFSISR +++WG P+PND    IYVWFDALLG                 
Sbjct: 195 EVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEEPNLTNA 254

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 123
                                                          DG +MGKSLGNT+
Sbjct: 255 LVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGKSLGNTV 314

[Close]
BLAST of Moc02g13170 vs. ExPASy Swiss-Prot
Match: Q98MV8 (Methionine--tRNA ligase OS=Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) OX=266835 GN=metG PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.6e-22
Identity = 58/175 (33.14%), Postives = 82/175 (46.86%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDA-------------------- 66
           +V S+++SGL+D SISR + +WG+PVP D K ++YVW DA                    
Sbjct: 194 EVMSFVKSGLKDLSISRTTFKWGVPVPGDDKHVMYVWVDALTNYITAAGYPDTKSDQWRY 253

Query: 67  ---------------------------------------LLGDGTRMGKSLGNTLEPNEL 123
                                                  L   G +M KS+GN ++P  L
Sbjct: 254 WPATHIIGKDIVRFHAVYWPAFLMSAGIELPKRVFAHGFLFNRGEKMSKSVGNVVDPFAL 313

[Close]
BLAST of Moc02g13170 vs. ExPASy TrEMBL
Match: A0A6J1D8R1 (methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017961 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.2e-66
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0 

Query: 2   STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNT 61
           STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNT
Sbjct: 36  STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDGTRMGKSLGNT 95

Query: 62  LEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLM 121
           LEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLM
Sbjct: 96  LEPNELVQKFGSDAVRYFFLGKMEFGSDGDYAKDRFINIVNAHLANTLGNLLNRTLRLLM 155

Query: 122 KTAKQLW 129
           KTAKQLW
Sbjct: 156 KTAKQLW 162

[Close]
BLAST of Moc02g13170 vs. ExPASy TrEMBL
Match: A0A6J1D617 (methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017961 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 6.6e-61
Identity = 127/160 (79.38%), Postives = 127/160 (79.38%), Query Frame = 0 

Query: 2   STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG------------ 61
           STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG            
Sbjct: 36  STISAKVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLGDILRFHAVYWPA 95

Query: 62  ---------------------DGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGS 121
                                DGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGS
Sbjct: 96  MLMSAELKLPKMVFDHGFLTMDGTRMGKSLGNTLEPNELVQKFGSDAVRYFFLGKMEFGS 155

Query: 122 DGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW 129
           DGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW
Sbjct: 156 DGDYAKDRFINIVNAHLANTLGNLLNRTLRLLMKTAKQLW 195

[Close]
BLAST of Moc02g13170 vs. ExPASy TrEMBL
Match: A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.3e-44
Identity = 103/183 (56.28%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWIRSGL+DFSISRASVEWGIPVPNDQKQ IYVWFDALLG                 
Sbjct: 263 EVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQQNLQRA 322

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 323 VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTL 382

[Close]
BLAST of Moc02g13170 vs. ExPASy TrEMBL
Match: A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.6e-43
Identity = 101/183 (55.19%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWIRSGL+DFSISRASVEWGIPVPND+KQ IYVWFDALLG                 
Sbjct: 258 EVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDALLGYISALSENGEQPNLQEV 317

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 318 VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTL 377

[Close]
BLAST of Moc02g13170 vs. ExPASy TrEMBL
Match: A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.1e-43
Identity = 101/183 (55.19%), Postives = 112/183 (61.20%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWIRSGL+DFSISRASVEWGIPVPND+KQ IYVWFDALLG                 
Sbjct: 258 EVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWFDALLGYISALSENGEQPNLHKV 317

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 318 VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTL 377

[Close]
BLAST of Moc02g13170 vs. TAIR 10
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 168.7 bits (426), Expect = 3.0e-42
Identity = 93/183 (50.82%), Postives = 106/183 (57.92%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWI+SGL DFSISRA V+WGIPVP+D KQ IYVWFDALLG                 
Sbjct: 256 EVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFDALLGYISALTEDNKQQNLETA 315

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 316 VSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMVFGHGFLTKDGMKMGKSLGNTL 375

[Close]
BLAST of Moc02g13170 vs. TAIR 10
Match: AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 168.7 bits (426), Expect = 3.0e-42
Identity = 93/183 (50.82%), Postives = 106/183 (57.92%), Query Frame = 0 

Query: 7   KVKSWIRSGLEDFSISRASVEWGIPVPNDQKQIIYVWFDALLG----------------- 66
           +V+SWI+SGL DFSISRA V+WGIPVP+D KQ IYVWFDALLG                 
Sbjct: 256 EVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFDALLGYISALTEDNKQQNLETA 315

Query: 67  -----------------------------------------------DGTRMGKSLGNTL 126
                                                          DG +MGKSLGNTL
Sbjct: 316 VSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMVFGHGFLTKDGMKMGKSLGNTL 375

The following BLAST results are available for this feature:
BLAST of Moc02g13170 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022149556.11.1e-65100.00methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 [Momordica ... [more]
XP_022149555.11.4e-6079.38methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Momordica ... [more]
XP_022133974.16.8e-4456.28methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
XP_004142742.13.4e-4355.19methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284... [more]
KAA0066020.14.4e-4355.19methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ... [more]
BLAST of Moc02g13170 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M2T94.3e-4150.82Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q8Z0683.4e-3040.00Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
P590815.8e-3040.00Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221... [more]
Q557291.3e-2937.78Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Q98MV82.6e-2233.14Methionine--tRNA ligase OS=Mesorhizobium japonicum (strain LMG 29417 / CECT 9101... [more]
BLAST of Moc02g13170 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D8R15.2e-66100.00methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X2 OS=Momordic... [more]
A0A6J1D6176.6e-6179.38methionine--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 OS=Momordic... [more]
A0A6J1BXG83.3e-4456.28Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1[more]
A0A0A0KQV71.6e-4355.19Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1[more]
A0A5D3C1K02.1e-4355.19Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
BLAST of Moc02g13170 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G55400.13.0e-4250.82methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT3G55400.23.0e-4250.82methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015413Methionyl/Leucyl tRNA synthetasePFAMPF09334tRNA-synt_1gcoord: 50..117
e-value: 2.6E-14
score: 52.8
coord: 4..49
e-value: 1.8E-13
score: 50.1
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 50..89
e-value: 2.1E-9
score: 38.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 2..48
e-value: 2.0E-17
score: 65.1
NoneNo IPR availableGENE3D1.10.730.10coord: 93..127
e-value: 8.8E-6
score: 27.5
NoneNo IPR availablePANTHERPTHR43326METHIONYL-TRNA SYNTHETASEcoord: 50..124
NoneNo IPR availablePANTHERPTHR43326:SF6BNAA09G34980D PROTEINcoord: 7..49
NoneNo IPR availablePANTHERPTHR43326METHIONYL-TRNA SYNTHETASEcoord: 7..49
NoneNo IPR availablePANTHERPTHR43326:SF6BNAA09G34980D PROTEINcoord: 50..124
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 7..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g13170.1Moc02g13170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006431 methionyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004825 methionine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding