Moc02g12530 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g12530
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Polynucleotidyl transferase, ribonuclease H-like superfamily protein
Location	chr2: 9107180 .. 9107488 (-)
RNA-Seq Expression	Moc02g12530
Synteny	Moc02g12530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGCTACCTGGATGGACTCCCTGAATCGTGGTGGCATCGGCTGGATTCTCCGCGACTCTAATGGCTCGATGATTTCGACTGGGTGTAGGCTTGTGCATCACAGATGGCAAATCAAGGTTTTGGAGGCGATGACTGTGGTTGAAGGTTTGAAATCTATTAACAAGGAAGAAATGTTAGAAACCATTGTTGAGTTGGACTGCCTTGAACTAGTTAATCTGCTGAAAGGGGTGAATTCCGACCTAACTGAGATTAGCTTTTCATCGACGAAGCTTTGCGTCTACAAGAGGTCTAGAGGACTGTCGTGA

mRNA sequence

ATGGACGCTACCTGGATGGACTCCCTGAATCGTGGTGGCATCGGCTGGATTCTCCGCGACTCTAATGGCTCGATGATTTCGACTGGGTGTAGGCTTGTGCATCACAGATGGCAAATCAAGGTTTTGGAGGCGATGACTGTGGTTGAAGGTTTGAAATCTATTAACAAGGAAGAAATGTTAGAAACCATTGTTGAGTTGGACTGCCTTGAACTAGTTAATCTGCTGAAAGGGGTGAATTCCGACCTAACTGAGATTAGCTTTTCATCGACGAAGCTTTGCGTCTACAAGAGGTCTAGAGGACTGTCGTGA

Coding sequence (CDS)

ATGGACGCTACCTGGATGGACTCCCTGAATCGTGGTGGCATCGGCTGGATTCTCCGCGACTCTAATGGCTCGATGATTTCGACTGGGTGTAGGCTTGTGCATCACAGATGGCAAATCAAGGTTTTGGAGGCGATGACTGTGGTTGAAGGTTTGAAATCTATTAACAAGGAAGAAATGTTAGAAACCATTGTTGAGTTGGACTGCCTTGAACTAGTTAATCTGCTGAAAGGGGTGAATTCCGACCTAACTGAGATTAGCTTTTCATCGACGAAGCTTTGCGTCTACAAGAGGTCTAGAGGACTGTCGTGA

Protein sequence

MDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLETIVELDCLELVNLLKGVNSDLTEISFSSTKLCVYKRSRGLS

Homology

[Close]

BLAST of Moc02g12530 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 1.3e-08
Identity = 35/84 (41.67%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           +A W    N GGIGWILRD  G +I   CR++     I  LE M + EGL++I +E    
Sbjct: 92  NAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCRP 151

Query: 62  TIVELDCLELVNLLKGVNSDLTEI 86
             +E D LE ++LL     D TEI
Sbjct: 152 IHLESDSLEAIHLLHRQCQDQTEI 175

[Close]

BLAST of Moc02g12530 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 69.3 bits (168), Expect = 2.1e-08
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DA W    N  GIGWILRD  G +I TGCR++     I  LE M + EGL++I +E    
Sbjct: 268 DAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQEHCRP 327

Query: 62  TIVELDCLELVNLL 76
             +E D LE ++LL
Sbjct: 328 IHLESDSLEAIHLL 341

[Close]

BLAST of Moc02g12530 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 69.3 bits (168), Expect = 2.1e-08
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DA W    N  GIGWILRD  G +I TGCR++     I  LE M + EGL++I +E    
Sbjct: 233 DAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQEHCRP 292

Query: 62  TIVELDCLELVNLL 76
             +E D LE ++LL
Sbjct: 293 IHLESDSLEAIHLL 306

[Close]

BLAST of Moc02g12530 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 65.5 bits (158), Expect = 3.1e-07
Identity = 33/84 (39.29%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DA+W DS +RGGIGWI+R  +G ++  G R V     +K+LEA  ++EGL+++    +L 
Sbjct: 84  DASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLR 143

Query: 62  TI-VELDCLELVNLLKGVNSDLTE 85
            + +E D  E+ +LL     DLT+
Sbjct: 144 PLHIETDSAEVESLLNRKREDLTK 167

[Close]

BLAST of Moc02g12530 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 65.1 bits (157), Expect = 4.1e-07
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKE---- 61
           DA W    N GGIGWILRD  G +I   CR++     I  LE M + EGL++I +E    
Sbjct: 92  DAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRP 151

Query: 62  ---EMLETI-VELDCLELVNLLKGVNSDLTEI 86
              E    I +E D LE ++LL     D TEI
Sbjct: 152 IQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 183

[Close]

BLAST of Moc02g12530 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 6.1e-09
Identity = 35/84 (41.67%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           +A W    N GGIGWILRD  G +I   CR++     I  LE M + EGL++I +E    
Sbjct: 92  NAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCRP 151

Query: 62  TIVELDCLELVNLLKGVNSDLTEI 86
             +E D LE ++LL     D TEI
Sbjct: 152 IHLESDSLEAIHLLHRQCQDQTEI 175

[Close]

BLAST of Moc02g12530 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-08
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DA W    N  GIGWILRD  G +I TGCR++     I  LE M + EGL++I +E    
Sbjct: 268 DAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQEHCRP 327

Query: 62  TIVELDCLELVNLL 76
             +E D LE ++LL
Sbjct: 328 IHLESDSLEAIHLL 341

[Close]

BLAST of Moc02g12530 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-08
Identity = 33/74 (44.59%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DA W    N  GIGWILRD  G +I TGCR++     I  LE M + EGL++I +E    
Sbjct: 233 DAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQEHCRP 292

Query: 62  TIVELDCLELVNLL 76
             +E D LE ++LL
Sbjct: 293 IHLESDSLEAIHLL 306

[Close]

BLAST of Moc02g12530 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.5e-07
Identity = 33/84 (39.29%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DA+W DS +RGGIGWI+R  +G ++  G R V     +K+LEA  ++EGL+++    +L 
Sbjct: 84  DASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLR 143

Query: 62  TI-VELDCLELVNLLKGVNSDLTE 85
            + +E D  E+ +LL     DLT+
Sbjct: 144 PLHIETDSAEVESLLNRKREDLTK 167

[Close]

BLAST of Moc02g12530 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.0e-07
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKE---- 61
           DA W    N GGIGWILRD  G +I   CR++     I  LE M + EGL++I +E    
Sbjct: 92  DAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEHCRP 151

Query: 62  ---EMLETI-VELDCLELVNLLKGVNSDLTEI 86
              E    I +E D LE ++LL     D TEI
Sbjct: 152 IQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 183

[Close]

BLAST of Moc02g12530 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 40.8 bits (94), Expect = 7.6e-04
Identity = 27/80 (33.75%), Postives = 39/80 (48.75%), Query Frame = 0


Query: 2   DATWMDSLNRGGIGWILRDSNGSMISTGCRLVHHRWQIKVLEAMTVVEGLKSINKEEMLE 61
           DATW     R GIGWILR+ +G ++  G R +     +   E   +   + ++++     
Sbjct: 150 DATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRFNYKR 209

Query: 62  TIVELDCLELVNLLKGVNSD 82
            I E D   LVNLL   NSD
Sbjct: 210 IIFESDAQALVNLL---NSD 226

The following BLAST results are available for this feature:

BLAST of Moc02g12530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143535.1	1.3e-08	41.67	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022154990.1	2.1e-08	44.59	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022154991.1	2.1e-08	44.59	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]
XP_022155262.1	3.1e-07	39.29	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022156777.1	4.1e-07	41.30	uncharacterized protein LOC111023608 [Momordica charantia]	[more]

BLAST of Moc02g12530 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc02g12530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	6.1e-09	41.67	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DL64	1.0e-08	44.59	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQC9	1.0e-08	44.59	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DNV9	1.5e-07	39.29	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DSV1	2.0e-07	41.30	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Moc02g12530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	7.6e-04	33.75	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 2..80 e-value: 7.8E-13 score: 48.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g12530.1	Moc02g12530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity