Homology
BLAST of Moc02g12470 vs. NCBI nr
Match:
XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])
HSP 1 Score: 1000.7 bits (2586), Expect = 4.3e-288
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG
Sbjct: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG
Sbjct: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA
Sbjct: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP
Sbjct: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL
Sbjct: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK
Sbjct: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
Query: 481 CKPRPIMKLILNEAIIT 498
CKPRPIMKLILNEAIIT
Sbjct: 481 CKPRPIMKLILNEAIIT 497
BLAST of Moc02g12470 vs. NCBI nr
Match:
XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])
HSP 1 Score: 799.3 bits (2063), Expect = 1.9e-227
Identity = 389/497 (78.27%), Postives = 445/497 (89.54%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSISLSLFSL + F FL R R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH
Sbjct: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
YGHVFSLR+GSR+VVVVSSPSAVQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI TLEIFST+RLN+FLGIREDEV+NL+ KL +S +EF AVEL+ ML D TF
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
Query: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240
NIV+RM+AGKKYY +E QSKKFREMV ++LA+ G N GDF+P+WNWIDPTG+EKR
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
Query: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300
I+K+G+RSDELLQEL+DEIRNQ+ G NTMIDHLL LQ +PEY+SDQIIKGL Q++LLAG
Sbjct: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
Query: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360
IDTAA+TLEWALS+LLNNPEVL+KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRL
Sbjct: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
Query: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI-G 420
NPAAPLL+PHCAS DCKI GYD+PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ G
Sbjct: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
Query: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480
VDSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEWER+++SLVDMTEG+GLTMPKA
Sbjct: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
Query: 481 QHLVAKCKPRPIMKLIL 492
Q LVAKCKPRPI+K IL
Sbjct: 561 QPLVAKCKPRPILKAIL 577
BLAST of Moc02g12470 vs. NCBI nr
Match:
XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])
HSP 1 Score: 778.9 bits (2010), Expect = 2.7e-221
Identity = 376/495 (75.96%), Postives = 433/495 (87.47%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSI L L SLL AF+FL R NLPP+PLFSLP++GH HL+KHP+H++ H LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
K+GHV SLRFGSR+VVV+SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+AV+PYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI +LEIFST RLNMF+GIRE+EV+ L+ KLC + EEF VELEPML DLTF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
N+VM MV G+++Y+E SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L
Sbjct: 181 NVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
+R DELLQELIDE R+ + G NTMI HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+A
Sbjct: 241 RRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALS LLNNP+VL+KA+AEID +G+ERLVNE+DL +L YLQGII ETLRLNPAAP
Sbjct: 301 VTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LL+PHCA DCKI GYDVPRDTI+LIN WAIHRDPNLWED T FKPERHG+T G DSYKL
Sbjct: 361 LLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSGADSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFG GRR+CPGVGMAQRVVGL LASLIQGFEWER+S+SLVDM+EG+GLTMPKAQ LVAK
Sbjct: 421 LPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAK 480
Query: 481 CKPRPIMKLILNEAI 496
CKPRPIMK I+N I
Sbjct: 481 CKPRPIMKAIMNVEI 495
BLAST of Moc02g12470 vs. NCBI nr
Match:
XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 774.6 bits (1999), Expect = 5.1e-220
Identity = 373/495 (75.35%), Postives = 432/495 (87.27%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSI L L SLL AF+FL R NLPP+PLFSLP++GH HL+KHP+H++ H LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHTLSQ 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
K+GHV SLRFGSR+VVV+SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+A++PYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI +LEIFST RLNMF+GIRE+EV+ L+ KLC EE VELEPML DLTF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
N+VM MV G+++Y+E SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L
Sbjct: 181 NVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
+R DE+LQELIDE R+ + G NTMI HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+A
Sbjct: 241 RRGDEVLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALS+LLNNP+VL+KA+AEID +G+ERLVNE+DL +L YLQGII ETLRLNPAAP
Sbjct: 301 VTLEWALSQLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LL+PHCA DCKI GYDVPRDTI+LIN WAIHRDPNLWED T FKPERHG+T G DSYKL
Sbjct: 361 LLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPNLWEDPTAFKPERHGDTSGADSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFG GRR+CPGVGMAQRVVGL LASLIQGFEWER+S+SLVDM+EG+GLTMPKAQ LVAK
Sbjct: 421 LPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAK 480
Query: 481 CKPRPIMKLILNEAI 496
CKPRPIMK I+N I
Sbjct: 481 CKPRPIMKAIMNVEI 495
BLAST of Moc02g12470 vs. NCBI nr
Match:
XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])
HSP 1 Score: 771.5 bits (1991), Expect = 4.3e-219
Identity = 369/495 (74.55%), Postives = 434/495 (87.68%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSI L L SLL AF+FL R NLPP+PLFSLP++GH HL+KHP+H++ H LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
K+GHV SLRFGSR+VV++SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+A++PYG
Sbjct: 61 KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI +LEIFST+RLN+FLGIR++EV+ L+ KLC + EEF VE+EPML DLTF
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
N+VM MV G+++Y+E SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L
Sbjct: 181 NVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
+R DELLQELIDE R+ + G N+MI HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+A
Sbjct: 241 RRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALS LLNNP+VL+KA+AEID +G+ERLVNE+DL +L YLQGII ETLRLNPAAP
Sbjct: 301 VTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LL+PHCA DCKI GYDVPRDTI+LIN WAIHRDP+LWED T FKPERHG++ G DSYKL
Sbjct: 361 LLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDSSGADSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFG GRR+CPGVGMAQRVVGL LASLIQGFEWER+S+SLVDM+EG+GLTMPKAQ LVAK
Sbjct: 421 LPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAK 480
Query: 481 CKPRPIMKLILNEAI 496
CKPRPIMK I+N I
Sbjct: 481 CKPRPIMKAIMNVEI 495
BLAST of Moc02g12470 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 571.2 bits (1471), Expect = 1.1e-161
Identity = 279/495 (56.36%), Postives = 366/495 (73.94%), Query Frame = 0
Query: 3 ILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKY 62
++ LSISL LF + RR+NLPPSP +LP++GHLHL+ +H++ ++LS KY
Sbjct: 13 VVLLSISLKLFPV--------SRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72
Query: 63 GHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEH 122
G VFSL+ G+R+V+VVSSP+A +ECFTKNDIV ANRPL GK + YN+TTM +PYGEH
Sbjct: 73 GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132
Query: 123 WRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNI 182
WRNLRR+ +EIFS LN FL IREDEV+ L+L L ++S ++F VE++ L +L+FN+
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 192
Query: 183 VMRMVAGKKYY----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 242
MRMVAGK+Y+ + ++K FR ++ +V H GA+NPGDF+P WID +EK++ K
Sbjct: 193 TMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSK 252
Query: 243 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 302
+ Q D LQ LI E R + + TMIDHLL LQ+++PEYY+DQIIKG+I V+LLAG DT
Sbjct: 253 ISQEMDAFLQRLIHESRINK-NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDT 312
Query: 303 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 362
+AVT+EWA+S LLN+PE LEKA+ EI+ VG RL+ E DL L YL II ET RL PA
Sbjct: 313 SAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPA 372
Query: 363 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSY 422
AP+L+PH +S DCK+ GYDVP+ TI+L+N WAIHRDP W++ T FKPERHG + ++
Sbjct: 373 APMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGG-VELEPS 432
Query: 423 KLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLV 482
KL+PFG GRRSCPG G+AQRVVGLTL +LIQ FEW+R+ + +DM EG GLTMPKAQ L
Sbjct: 433 KLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLE 492
Query: 483 AKCKPRPIMKLILNE 494
A CKPR I+ +++E
Sbjct: 493 ALCKPRNILHKVVSE 497
BLAST of Moc02g12470 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 530.0 bits (1364), Expect = 2.8e-149
Identity = 265/498 (53.21%), Postives = 355/498 (71.29%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRR--QNLPPSPLFSLPIVGHLHLVKHPVHQTFHEL 60
M +LS+ +SLF L++ F + R +NLPP P LPI+G+LH +K P+H TFH L
Sbjct: 1 MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTL 60
Query: 61 SHKYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAP 120
S KYG +FSL FGSR+VVVVSS + QECFTKNDIVLANRP +GK + YN+TT+A +P
Sbjct: 61 SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120
Query: 121 YGEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDL 180
YG+HWRNLRRI ++EI S+ RLN FL IR DE+ L+ KL + S+ FT VEL PM ++
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEM 180
Query: 181 TFNIVMRMVAGKKYY-------EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTG 240
TFN +MRMV+GK+YY + ++++ FR ++ +V++ GA N GDF+ W D G
Sbjct: 181 TFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG 240
Query: 241 FEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVI 300
EKR+ K+ +R+D LQ LIDE R + NTMIDHLL Q+++PEYY+DQIIKGL+ V+
Sbjct: 241 LEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300
Query: 301 LLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWE 360
LLAG DT++VT+EWA+S LLN+PE+++KAK E+D +G +R V+E D+S L YLQ I++E
Sbjct: 301 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 360
Query: 361 TLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGN 420
TLRL+ AAPLL+PH +S D +GGY++P++TI+++N W IHRDPNLW D T FKPER
Sbjct: 361 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 420
Query: 421 TIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTM 480
V+ KLL FG GRR+CPG ++QR GLTL LIQ FEW+R+ +DM E KG+T
Sbjct: 421 EGEVN--KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITA 480
Query: 481 PKAQHLVAKCKPRPIMKL 490
K L A CK R +K+
Sbjct: 481 GKKTSLNAMCKVRHPLKI 495
BLAST of Moc02g12470 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 521.5 bits (1342), Expect = 1.0e-146
Identity = 270/493 (54.77%), Postives = 352/493 (71.40%), Query Frame = 0
Query: 11 SLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH-----V 70
SL L+++F FL + ++QNLPPSP LPI+GHL L+K P+H+T S H V
Sbjct: 15 SLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGV 74
Query: 71 FSLRFGSRVVVVVSSPS-AVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWR 130
SLR GSR+V VVSS A +ECF KND+VLANRP + GK + YN+T M APYG+HWR
Sbjct: 75 MSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWR 134
Query: 131 NLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVM 190
NLRR+ T+EIFST RLN FL +R DEVR L+ +L + + T VEL+PML DLTFN +M
Sbjct: 135 NLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIM 194
Query: 191 RMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKL 250
RM+ GK+YY +E+++K+ R++V V A+ + N D++PI + +E R+ KL
Sbjct: 195 RMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKL 254
Query: 251 GQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTA 310
G+ +D+ LQ LID+ R Q+ TMIDHLL LQK++ EYY+DQIIKG+I ++++AG +T+
Sbjct: 255 GEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTS 314
Query: 311 AVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAA 370
AVTLEWALS LLN+P+V+ KA+ EID VG +RL+ EADLS L YL+ I+ ETLRL+PA
Sbjct: 315 AVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPAT 374
Query: 371 PLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYK 430
PLL+PH AS DCKIG YD+PR T +L+N WAIHRDPN W+D +FKPER ++ K
Sbjct: 375 PLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE--EEAQK 434
Query: 431 LLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVA 490
LL FG GRR+CPG G+AQR+VGL L SLIQ FEWER+ N VDM EG G T+PKA L A
Sbjct: 435 LLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKA 494
Query: 491 KCKPRPIMKLILN 493
CK RP + I++
Sbjct: 495 ICKARPFLHKIIS 502
BLAST of Moc02g12470 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 514.6 bits (1324), Expect = 1.2e-144
Identity = 263/493 (53.35%), Postives = 349/493 (70.79%), Query Frame = 0
Query: 9 SLSLFSLLLAFSFLFR--SRR-QNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHV 68
SL S ++ L + SRR +NLPP P ++PI+G+LH +KHP+H+TF LS YG +
Sbjct: 6 SLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYGDI 65
Query: 69 FSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRN 128
FSL FGSR+VVVVSSPS ECFTKNDI+LANRP +GK + YN+TT+ A YG+HWRN
Sbjct: 66 FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 125
Query: 129 LRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEE---FTAVELEPMLFDLTFNI 188
LRRI T+++ S RLN FLG+R DE L+ KL ++ E FT VEL P L ++TFN
Sbjct: 126 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 185
Query: 189 VMRMVAGKKYY-------EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 248
+MRM++GK+YY + +++K+FRE+++++++ +GA N GDF+P+ +D EKR
Sbjct: 186 MMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKR 245
Query: 249 IIKLGQRSDELLQELIDEIR--NQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILL 308
++ +RS+ L+ LI+E R N TMIDHLL L +++PEYYSD +IKGLIQ +LL
Sbjct: 246 CKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLL 305
Query: 309 AGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETL 368
AG DT+AVT+EW +S LLN+PEVL+KAK E+D +G+ +LV+E DLS L YLQ II ETL
Sbjct: 306 AGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETL 365
Query: 369 RLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI 428
RL+P APLL+PH +S DC IG ++VP+DTIIL N+W IHRDP W DA +FKPER
Sbjct: 366 RLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEE 425
Query: 429 GVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPK 487
V+ K++ FG GRR+CPG+ +AQR VG T+ LIQ FEWER S +DM EGKG+TMP
Sbjct: 426 EVN--KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPM 485
BLAST of Moc02g12470 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 511.5 bits (1316), Expect = 1.0e-143
Identity = 260/502 (51.79%), Postives = 352/502 (70.12%), Query Frame = 0
Query: 1 MDIL-FLSISLSLFSLLLAFSFLFRSRR-QNLPPSPLFSLPIVGHLHLVKHPVHQTFHEL 60
M+IL LS S+ +L F+ + R+R+ +NLPP P SLPI+G+LH +K P+H+TF L
Sbjct: 1 MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60
Query: 61 SHKYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAP 120
S KYGHVFSL FGSR+VVVVSS S Q+CFTKND+VLANRP SGK + YN+TT+
Sbjct: 61 SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120
Query: 121 YGEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDL 180
YGEHWRNLRRI L++ S R+N F GIR DE + L+ +L ++S F +EL L+D+
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180
Query: 181 TFNIVMRMVAGKKYYEE-------QQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTG 240
TFN +MRM++GK+YY E Q++ +FR+MV+++L GA N DF+P+ ++D
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240
Query: 241 FEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVI 300
EKR+ + ++D L+ LI+E R ++ NTMIDHLL LQ ++PEYY+DQIIKGL +
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 300
Query: 301 LLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWE 360
LLAG D++AVTLEW++S LLN+PEVL+K K E+D VGQ+RLV+E+DL L YL+ +I E
Sbjct: 301 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 360
Query: 361 TLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGN 420
TLRL APLL+PH S +C IGGY VP+DTI+LIN WAIHRDP LW +AT FKPER
Sbjct: 361 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK 420
Query: 421 TIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTM 480
++ KL+ FG GRR+CPG G+A R + +TLA LIQ F+W+ ++ +D+ E G T+
Sbjct: 421 KGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTL 480
Query: 481 PKAQHLVAKCKPRPIMKLILNE 494
K L A CK RP++ + +
Sbjct: 481 TKLVPLKAMCKSRPVINKVFKQ 499
BLAST of Moc02g12470 vs. ExPASy TrEMBL
Match:
A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)
HSP 1 Score: 1000.7 bits (2586), Expect = 2.1e-288
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG
Sbjct: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG
Sbjct: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA
Sbjct: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP
Sbjct: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL
Sbjct: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK
Sbjct: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
Query: 481 CKPRPIMKLILNEAIIT 498
CKPRPIMKLILNEAIIT
Sbjct: 481 CKPRPIMKLILNEAIIT 497
BLAST of Moc02g12470 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 799.3 bits (2063), Expect = 9.3e-228
Identity = 389/497 (78.27%), Postives = 445/497 (89.54%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSISLSLFSL + F FL R R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH
Sbjct: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
YGHVFSLR+GSR+VVVVSSPSAVQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI TLEIFST+RLN+FLGIREDEV+NL+ KL +S +EF AVEL+ ML D TF
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
Query: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240
NIV+RM+AGKKYY +E QSKKFREMV ++LA+ G N GDF+P+WNWIDPTG+EKR
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
Query: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300
I+K+G+RSDELLQEL+DEIRNQ+ G NTMIDHLL LQ +PEY+SDQIIKGL Q++LLAG
Sbjct: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
Query: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360
IDTAA+TLEWALS+LLNNPEVL+KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRL
Sbjct: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
Query: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI-G 420
NPAAPLL+PHCAS DCKI GYD+PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ G
Sbjct: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500
Query: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480
VDSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEWER+++SLVDMTEG+GLTMPKA
Sbjct: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560
Query: 481 QHLVAKCKPRPIMKLIL 492
Q LVAKCKPRPI+K IL
Sbjct: 561 QPLVAKCKPRPILKAIL 577
BLAST of Moc02g12470 vs. ExPASy TrEMBL
Match:
A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)
HSP 1 Score: 778.9 bits (2010), Expect = 1.3e-221
Identity = 376/495 (75.96%), Postives = 433/495 (87.47%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSI L L SLL AF+FL R NLPP+PLFSLP++GH HL+KHP+H++ H LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
K+GHV SLRFGSR+VVV+SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+AV+PYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI +LEIFST RLNMF+GIRE+EV+ L+ KLC + EEF VELEPML DLTF
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
N+VM MV G+++Y+E SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L
Sbjct: 181 NVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
+R DELLQELIDE R+ + G NTMI HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+A
Sbjct: 241 RRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALS LLNNP+VL+KA+AEID +G+ERLVNE+DL +L YLQGII ETLRLNPAAP
Sbjct: 301 VTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LL+PHCA DCKI GYDVPRDTI+LIN WAIHRDPNLWED T FKPERHG+T G DSYKL
Sbjct: 361 LLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSGADSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFG GRR+CPGVGMAQRVVGL LASLIQGFEWER+S+SLVDM+EG+GLTMPKAQ LVAK
Sbjct: 421 LPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAK 480
Query: 481 CKPRPIMKLILNEAI 496
CKPRPIMK I+N I
Sbjct: 481 CKPRPIMKAIMNVEI 495
BLAST of Moc02g12470 vs. ExPASy TrEMBL
Match:
A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)
HSP 1 Score: 771.5 bits (1991), Expect = 2.1e-219
Identity = 369/495 (74.55%), Postives = 434/495 (87.68%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSI L L SLL AF+FL R NLPP+PLFSLP++GH HL+KHP+H++ H LS
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
K+GHV SLRFGSR+VV++SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+A++PYG
Sbjct: 61 KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
EHWRNLRRI +LEIFST+RLN+FLGIR++EV+ L+ KLC + EEF VE+EPML DLTF
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180
Query: 181 NIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLG 240
N+VM MV G+++Y+E SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L
Sbjct: 181 NVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 240
Query: 241 QRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAA 300
+R DELLQELIDE R+ + G N+MI HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+A
Sbjct: 241 RRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSA 300
Query: 301 VTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAP 360
VTLEWALS LLNNP+VL+KA+AEID +G+ERLVNE+DL +L YLQGII ETLRLNPAAP
Sbjct: 301 VTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPAAP 360
Query: 361 LLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKL 420
LL+PHCA DCKI GYDVPRDTI+LIN WAIHRDP+LWED T FKPERHG++ G DSYKL
Sbjct: 361 LLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDSSGADSYKL 420
Query: 421 LPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAK 480
LPFG GRR+CPGVGMAQRVVGL LASLIQGFEWER+S+SLVDM+EG+GLTMPKAQ LVAK
Sbjct: 421 LPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAK 480
Query: 481 CKPRPIMKLILNEAI 496
CKPRPIMK I+N I
Sbjct: 481 CKPRPIMKAIMNVEI 495
BLAST of Moc02g12470 vs. ExPASy TrEMBL
Match:
A0A6J1D8L6 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV=1)
HSP 1 Score: 760.8 bits (1963), Expect = 3.7e-216
Identity = 377/498 (75.70%), Postives = 422/498 (84.74%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
MDIL LSI L SLLLAF RR NLPPSPLFSLPI+GHLHLVKH VH+T H LSH
Sbjct: 1 MDILLLSIFL---SLLLAFLLFRPHRRNNLPPSPLFSLPIIGHLHLVKHSVHRTLHNLSH 60
Query: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
YG VFSLRFGSR+VVVVSSPS VQECFTKNDI+LANRPLL + K LAYN TTMAVAPYG
Sbjct: 61 NYGRVFSLRFGSRLVVVVSSPSVVQECFTKNDIILANRPLLDTAKHLAYNQTTMAVAPYG 120
Query: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
+HWR+LRRIG LEIFSTTR+N+FLGIRE EV+ LM KLC +SF EFT VE M+ DL F
Sbjct: 121 DHWRHLRRIGALEIFSTTRMNLFLGIREGEVKRLMRKLCGSSFVEFTVVEPGSMILDLMF 180
Query: 181 NIVMRMVAGKKYYEEQ-----QSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240
NI+MR+VAGKKYY++ +S+KFRE V + +AH GATNPGDFIPIWNWIDPTG EK+
Sbjct: 181 NIIMRIVAGKKYYDDDMSNDGKSRKFREAVKETMAHGGATNPGDFIPIWNWIDPTGLEKK 240
Query: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300
++KLG DELLQEL+DEIRNQ+ NTMI+HLLCLQK+EPE +SD IIKGL+ VIL+AG
Sbjct: 241 MMKLGHTMDELLQELLDEIRNQKNEGNTMIEHLLCLQKSEPENHSDLIIKGLVHVILIAG 300
Query: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360
IDT VTLEWALS LLNNPEVLEKAKAEID +GQER+VNEADLSSL Y+QGII+ETLRL
Sbjct: 301 IDTIGVTLEWALSHLLNNPEVLEKAKAEIDNSIGQERMVNEADLSSLSYVQGIIFETLRL 360
Query: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGV 420
PAAPLL+ HC S DCKIGGYD+PRD I+LIN WAIHRDP LWEDAT+F+PERH N IGV
Sbjct: 361 TPAAPLLVAHCPSEDCKIGGYDIPRDAIVLINAWAIHRDPELWEDATSFRPERHTNAIGV 420
Query: 421 DSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQ 480
DSYKLLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEW+R S+ LVDMTEG GLTMPKAQ
Sbjct: 421 DSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWKRASSLLVDMTEGGGLTMPKAQ 480
Query: 481 HLVAKCKPRPIMKLILNE 494
LVAKC+PRPIM+ ILNE
Sbjct: 481 PLVAKCRPRPIMRAILNE 495
BLAST of Moc02g12470 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 521.5 bits (1342), Expect = 7.2e-148
Identity = 270/493 (54.77%), Postives = 352/493 (71.40%), Query Frame = 0
Query: 11 SLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH-----V 70
SL L+++F FL + ++QNLPPSP LPI+GHL L+K P+H+T S H V
Sbjct: 15 SLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGV 74
Query: 71 FSLRFGSRVVVVVSSPS-AVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWR 130
SLR GSR+V VVSS A +ECF KND+VLANRP + GK + YN+T M APYG+HWR
Sbjct: 75 MSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWR 134
Query: 131 NLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVM 190
NLRR+ T+EIFST RLN FL +R DEVR L+ +L + + T VEL+PML DLTFN +M
Sbjct: 135 NLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIM 194
Query: 191 RMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKL 250
RM+ GK+YY +E+++K+ R++V V A+ + N D++PI + +E R+ KL
Sbjct: 195 RMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKL 254
Query: 251 GQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTA 310
G+ +D+ LQ LID+ R Q+ TMIDHLL LQK++ EYY+DQIIKG+I ++++AG +T+
Sbjct: 255 GEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTS 314
Query: 311 AVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAA 370
AVTLEWALS LLN+P+V+ KA+ EID VG +RL+ EADLS L YL+ I+ ETLRL+PA
Sbjct: 315 AVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPAT 374
Query: 371 PLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYK 430
PLL+PH AS DCKIG YD+PR T +L+N WAIHRDPN W+D +FKPER ++ K
Sbjct: 375 PLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE--EEAQK 434
Query: 431 LLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVA 490
LL FG GRR+CPG G+AQR+VGL L SLIQ FEWER+ N VDM EG G T+PKA L A
Sbjct: 435 LLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKA 494
Query: 491 KCKPRPIMKLILN 493
CK RP + I++
Sbjct: 495 ICKARPFLHKIIS 502
BLAST of Moc02g12470 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 521.2 bits (1341), Expect = 9.4e-148
Identity = 263/499 (52.71%), Postives = 360/499 (72.14%), Query Frame = 0
Query: 4 LFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYG 63
L SI + SL+ L R+ NLPPSP +SLP++GHL L+K P+H+TF LS
Sbjct: 6 LIFSILFVVLSLIYLIGKL--KRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65
Query: 64 H--VFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGE 123
+ +FSLR G+R+V V SS S +ECFTKND+VLANRP K +AY++TTM A YG+
Sbjct: 66 NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125
Query: 124 HWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFN 183
HWRNLRRIG++EIFS RLN FL IR+DE+R L+ +L N +EF V+++ ML DLTFN
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 185
Query: 184 IVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRI 243
++RMVAGK+YY ++ ++K+ R+++ V+A GA N D++P+ + + +E R+
Sbjct: 186 NILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRV 245
Query: 244 IKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGI 303
KL R DE LQ L+DE R + NTMIDHLL LQ+++P+Y++D+IIKG + ++LAG
Sbjct: 246 KKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 305
Query: 304 DTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLN 363
DT+AVTLEWALS +LN+P+VL KA+ EID +G +RL++E+D+S+L YLQ I+ ETLRL
Sbjct: 306 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 365
Query: 364 PAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVD 423
PAAP+L+PH AS DCK+ GYD+PR TI+L N+WAIHRDP LW+D +FKPER +
Sbjct: 366 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKE--GE 425
Query: 424 SYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQH 483
+ KL+PFG GRR+CPG G+A R++ LTL SLIQ EWE++ VDM+EGKG+TMPKA+
Sbjct: 426 AQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKP 485
Query: 484 LVAKCKPRPIMKLILNEAI 496
L A C+ RP + I NE++
Sbjct: 486 LEAMCRARPSVVKIFNESV 497
BLAST of Moc02g12470 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 513.5 bits (1321), Expect = 2.0e-145
Identity = 270/499 (54.11%), Postives = 349/499 (69.94%), Query Frame = 0
Query: 5 FLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH 64
FL ISLSL ++ RR NLPPSP ++LP++GHL L+K P+H+ F +S G
Sbjct: 10 FLFISLSLTFIIGRI-----KRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGD 69
Query: 65 --VFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEH 124
+ SLR G+R+V VVSS S +ECFTKND+VLANR + K ++Y TT+ A YG+H
Sbjct: 70 APIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDH 129
Query: 125 WRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNI 184
WRNLRRIG +EIFS RLN F IR DE+ L+ L NS EFT VE++ M +LTFN
Sbjct: 130 WRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNN 189
Query: 185 VMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRII 244
++RM+AGK YY ++ ++K+ RE++ + + GA N D++PI WI TG EKRI
Sbjct: 190 IIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIK 249
Query: 245 KLGQRSDELLQELIDEIR-NQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGI 304
K+ R DE LQ L+DE R +E NTM+DHLLCLQ+ +PEYY+D IIKG++ ++LAG
Sbjct: 250 KIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGT 309
Query: 305 DTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLN 364
DT+AVTLEW LS LLN+P++L KA+ EID VG RLV E+DLS L YLQ I+ E+LRL
Sbjct: 310 DTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLY 369
Query: 365 PAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVD 424
PA+PLL+PH AS DCK+GGY +PR T++L N WAIHRDP +W+D T+FKPER +
Sbjct: 370 PASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKE--GE 429
Query: 425 SYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTE-GKGLTMPKAQ 484
+ KLL FG GRR+CPG G+AQR+ LT+ SLIQ FEWER+ VDMTE G G+ MPKA
Sbjct: 430 AQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAI 489
Query: 485 HLVAKCKPRPIMKLILNEA 495
LVA CK RP++ ILNE+
Sbjct: 490 PLVAMCKARPVVGKILNES 499
BLAST of Moc02g12470 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 513.5 bits (1321), Expect = 2.0e-145
Identity = 256/494 (51.82%), Postives = 348/494 (70.45%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
+ LFL+IS+ LLL S +R+ NLPPSP + LP++GHLHL+K PVH+TFH +S
Sbjct: 9 LSFLFLTISI---KLLLTKS----NRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISK 68
Query: 61 KYGH--VFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAP 120
G+ +F LR G+R+V V+SS S +ECFTKND+VLANRP + K + YN T M A
Sbjct: 69 SLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAAS 128
Query: 121 YGEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDL 180
YG+HWRNLRRI +EIFS+ R++ F IR+DE+R L+ L +S F VEL+ +L +L
Sbjct: 129 YGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNL 188
Query: 181 TFNIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFE 240
FN ++ MVAGK+YY + ++K RE++ +++A G+ N D++P NW+ T FE
Sbjct: 189 AFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFE 248
Query: 241 KRIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILL 300
+ LG R D +LQ+L+DE R ++ T+IDHLL Q+ EPEYY+D IIKG+I ++L
Sbjct: 249 NQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVL 308
Query: 301 AGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETL 360
AG DT++VTLEWA+S LLN+PE+LEKA+AEID +G +RLV E+D+ +L YLQ I+ ETL
Sbjct: 309 AGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETL 368
Query: 361 RLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTI 420
RL PA PLL+PH +S +CK+ GYD+PR T++L N+WA+HRDP LWE+ FKPER
Sbjct: 369 RLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKE- 428
Query: 421 GVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPK 480
++ KL+PFG GRR+CPG + +R+V L L LIQ FEWER+ LVDMTEG+G+TMPK
Sbjct: 429 -GEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPK 488
Query: 481 AQHLVAKCKPRPIM 488
A L A CK R I+
Sbjct: 489 ATPLRAMCKARAIV 491
BLAST of Moc02g12470 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 511.1 bits (1315), Expect = 9.7e-145
Identity = 268/502 (53.39%), Postives = 347/502 (69.12%), Query Frame = 0
Query: 1 MDILFLSISLSLFSLLLAFSFLF--RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHEL 60
M+ ++L +SL F + L+ LF R R+ NLPPSP PI+GHLHL+K P+H+ F L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 61 SHKYGH--VFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAV 120
S + +FSL GSR+V VVSS + +ECFTKND+VLANRP GK + YN TTM
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 121 APYGEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLF 180
A YG+ WRNLRRIGT+EIFS+ RLN F+ IR+DE+R L++ L +NS F VE++P+
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187
Query: 181 DLTFNIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTG 240
LT N ++RMVAGK++Y + ++K R+++ +V+ GA N D+ PI ++ T
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 247
Query: 241 FEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVI 300
+EK + KL R DE LQ L++E R ++ NTMIDHLL LQ+ +P+YY+D IIKG+I V+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307
Query: 301 LLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWE 360
+LAG DT+A TLEWA+S LLN+PEVL KAK EID +G +RLV E D+ L YLQ I+ E
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367
Query: 361 TLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGN 420
TLRL P AP+L+PH AS DC + GYDVPR TIIL+N WAIHRDP LWE+ FKPER
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427
Query: 421 TIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEG-KGLT 480
G D KL+PFG GRRSCPG G+AQR+V L L SL+Q FEWER+ +DM E KG T
Sbjct: 428 K-GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTT 487
Query: 481 MPKAQHLVAKCKPRPIMKLILN 493
M KA L A CK RPI+ +L+
Sbjct: 488 MRKATSLQAMCKARPIVHKVLD 505
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 1.1e-161 | 56.36 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ8 | 2.8e-149 | 53.21 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| Q9FG65 | 1.0e-146 | 54.77 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
| Q6WNQ9 | 1.2e-144 | 53.35 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| P93147 | 1.0e-143 | 51.79 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D5S1 | 2.1e-288 | 100.00 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... | [more] |
| A0A6J1D5S6 | 9.3e-228 | 78.27 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
| A0A6J1G5P7 | 1.3e-221 | 75.96 | cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1 | [more] |
| A0A6J1L2W8 | 2.1e-219 | 74.55 | cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1 | [more] |
| A0A6J1D8L6 | 3.7e-216 | 75.70 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV... | [more] |
