Moc02g12430 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc02g12430 vs. NCBI nr
Match: XP_022149524.1 (isoflavone 3'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 1012.3 bits (2616), Expect = 1.5e-291
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK
Sbjct: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
           KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG
Sbjct: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF
Sbjct: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK
Sbjct: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA
Sbjct: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR
Sbjct: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420
           LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR
Sbjct: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420

Query: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480
           PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA
Sbjct: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480

Query: 481 QPLIAKCKPHPIMKVILSKNN 502
           QPLIAKCKPHPIMKVILSKNN
Sbjct: 481 QPLIAKCKPHPIMKVILSKNN 501

BLAST of Moc02g12430 vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 766.9 bits (1979), Expect = 1.1e-217
Identity = 365/498 (73.29%), Postives = 434/498 (87.15%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           M+ LLLSI  SLFSL I   FL RP R NLPPSP F LPLIGH+HLVKHPVHRT HK+S 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
            +G +FSLR+GSRL+VVVSS SAVQECFTKNDIVLANRP++KSGK+LAYN+TTL  SPYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           EHWRNLRRIS LE+ STSRLN F+GIREDE+K L+ +L GDSL+EF  VE++ + LD TF
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           +IV+RM+AGKKYYG+D+  D  QS+KFRE+V +ILA+  + N GDF+P+WN IDP+G EK
Sbjct: 261 NIVVRMIAGKKYYGEDL-RDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEK 320

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           RIMK+G+R+DEL Q L+DEIRNQ +GGNTMIDHLL LQNTQPEY+SDQ+IKG+ Q ++LA
Sbjct: 321 RIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 380

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDT+A+T+EWALS LLNNP+VLKKAR EID+ IG++R+V+E DLSSLSYLQG+I+ETLR
Sbjct: 381 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 440

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI- 420
           L P  P+L+PHCASEDCK+EGYDIPRDTI+L+NAWAIHRD N+WEDA SFKPERH N + 
Sbjct: 441 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 500

Query: 421 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 480
             ++Y+LLPFG+GRRACPGMGMAQRVVGLTLASLIQCFEW+RV+SSLVDMTEGEG TMPK
Sbjct: 501 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 560

Query: 481 AQPLIAKCKPHPIMKVIL 498
           AQPL+AKCKP PI+K IL
Sbjct: 561 AQPLVAKCKPRPILKAIL 577

BLAST of Moc02g12430 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 751.9 bits (1940), Expect = 3.5e-213
Identity = 359/499 (71.94%), Postives = 428/499 (85.77%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           M+ L LSI  SLFSLL+  SFL R RR NLPPSP F+LP++GH+HLVKHPVH+TFH++S 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
           K+G +FSLRFGSR++VVVSS SAVQECFTKNDIVLANRP++KSGK LAYN+TT+  +PYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           EHWRNLRRI  LE+ ST+RLN F+GIREDE++ LM +LC +S EEF  VE+EP+  DLTF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           +IVMRM+AGKKYY      + +QS+KFRE+VTQ+LAH  + NPGDFIP+WN IDP+G EK
Sbjct: 181 NIVMRMVAGKKYY------EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEK 240

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           RI+KLGQR+DEL Q LIDEIRNQ  G NTMIDHLL LQ  +PEYYSDQ+IKG+IQ I+LA
Sbjct: 241 RIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLA 300

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDT+AVT+EWALS LLNNP+VL+KA+ EID  +GQER+VNEADLSSL YLQGII ETLR
Sbjct: 301 GIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLR 360

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420
           L P  P+LIPHCAS DCK+ GYD+PRDTI+L+N WAIHRDPNLWEDA +FKPERH N I 
Sbjct: 361 LNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIG 420

Query: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480
            ++Y+LLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEW+R+S+SLVDMTEG+G TMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480

Query: 481 QPLIAKCKPHPIMKVILSK 500
           Q L+AKCKP PIMK+IL++
Sbjct: 481 QHLVAKCKPRPIMKLILNE 493

BLAST of Moc02g12430 vs. NCBI nr
Match: XP_004151923.1 (cytochrome P450 81Q32 [Cucumis sativus] >KAE8652307.1 hypothetical protein Csa_022053 [Cucumis sativus])

HSP 1 Score: 722.6 bits (1864), Expect = 2.3e-204
Identity = 355/500 (71.00%), Postives = 417/500 (83.40%), Query Frame = 0

Query: 1   MEFLLLSI-LSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKIS 60
           M+ LLLSI L SLFSLL+  +FL +PR  NLPPSP F+LPLIGH+HL++HPVHRT H +S
Sbjct: 1   MDLLLLSISLISLFSLLLAFNFLHQPRHKNLPPSPSFSLPLIGHLHLLRHPVHRTLHNLS 60

Query: 61  KKFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPY 120
           +K GP+ SLRFGS L VVVSSSS VQECFTKNDI+LANRP++KSGKYL+YNYT L  SPY
Sbjct: 61  EKLGPVLSLRFGSCLAVVVSSSSLVQECFTKNDIILANRPLVKSGKYLSYNYTNLVISPY 120

Query: 121 GEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLC-GDSLEEFAVVEVEPLFLDL 180
           GEHWRNLRRIS LE+ S SRLN FVG REDE+KRL+ +L  G S EEF VVE+ P+F+DL
Sbjct: 121 GEHWRNLRRISTLEIFSISRLNLFVGGREDEVKRLLCKLWDGYSFEEFKVVEMFPMFMDL 180

Query: 181 TFSIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGL 240
            F+I+MRM++GKK  GDD+ ++ R S+KF+E+V Q+LA A + NPGDFIP+WN IDP+  
Sbjct: 181 MFNILMRMMSGKKNCGDDLKDEER-SKKFKEMVKQVLAIAGAGNPGDFIPLWNWIDPTCY 240

Query: 241 EKRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDII 300
            KR+ KL + +DEL Q LIDEIRNQN+G NTMI HLLGLQNTQPE YSDQ+IKG+IQ I+
Sbjct: 241 NKRVKKLSKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVIL 300

Query: 301 LAGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISET 360
           LAGIDT+AVT+EWAL HLLNNP+VLKKA  EID  IGQE +V E DL  LSYLQGII ET
Sbjct: 301 LAGIDTAAVTLEWALCHLLNNPEVLKKATDEIDSSIGQECLVKEVDLLRLSYLQGIIFET 360

Query: 361 LRLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNA 420
           LRL P  P+L+PHCASEDCK+ GYD+PRDT VL+NAWAIHRDP+LWEDA SFKPERH NA
Sbjct: 361 LRLNPATPLLVPHCASEDCKIGGYDVPRDTTVLINAWAIHRDPSLWEDATSFKPERHENA 420

Query: 421 IRPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMP 480
              + Y+LLPFG+GRRACPG+GMAQRVV LTLASL+QCFEW+R+ +SLVDMTEGEG TMP
Sbjct: 421 NGVDAYKLLPFGLGRRACPGVGMAQRVVALTLASLLQCFEWQRLGNSLVDMTEGEGLTMP 480

Query: 481 KAQPLIAKCKPHPIMKVILS 499
           KAQPL AKC+P PIM  ILS
Sbjct: 481 KAQPLTAKCRPRPIMMKILS 499

BLAST of Moc02g12430 vs. NCBI nr
Match: XP_038902744.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 721.8 bits (1862), Expect = 3.9e-204
Identity = 349/504 (69.25%), Postives = 424/504 (84.13%), Query Frame = 0

Query: 1   MEFLLLS-ILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKIS 60
           M+F LL+ IL SL  LL+  SFLP+P R NLPP+P F LP IGH+HL+KHPVHRTF  +S
Sbjct: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60

Query: 61  KKFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPY 120
           +K+G +FSL+FGSRL+V+VSS SAVQECFTKNDI+LANRP +++GKYLAYN TTL  SPY
Sbjct: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120

Query: 121 GEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDS---LEEFAVVEVEPLFL 180
           GEHWRNLRRIS LE+ ST+RLNSF  IRE E+KRL+ +LCG++    +EF VVE+EP+ L
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180

Query: 181 DLTFSIVMRMLAGKKY--YGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLID 240
           DLTF+IVMRM+ G K+    +++ ED   S KFRE++TQ++ + VS NPGDFIP+W  ID
Sbjct: 181 DLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRID 240

Query: 241 PSGLEKRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGII 300
           P+   K++ KLG+RADEL Q L+DEIRNQ + GNTMI HLL LQNT PEYYSDQVIKG++
Sbjct: 241 PTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLV 300

Query: 301 QDIILAGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGI 360
           Q + LAG+DT+AVT+EWALS LLNNP+VLKKAR EID  IGQE +VNEADLSSL+YLQGI
Sbjct: 301 QVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGI 360

Query: 361 ISETLRLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPER 420
           I+ETLRL P  P+L+PHCASEDCK+ GYD+PRDTIVL+NAWAIHRD +LWEDA SFKPER
Sbjct: 361 ITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPER 420

Query: 421 HTNAIRPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEG 480
           + NA+  ++ +L+PFG+GRRACPGMGMAQRV+ LTLASLIQCFEW+R+S+SLVDMTEGEG
Sbjct: 421 YENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEG 480

Query: 481 TTMPKAQPLIAKCKPHPIMKVILS 499
            TMPKAQPLIAKC+P PIMK +LS
Sbjct: 481 LTMPKAQPLIAKCRPRPIMKEMLS 504

BLAST of Moc02g12430 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.6e-158
Identity = 268/499 (53.71%), Postives = 364/499 (72.95%), Query Frame = 0

Query: 4   LLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKFG 63
           LL + L+ +   + +  F    RR NLPPSP  ALP+IGH+HL+   +HR+ + +SKK+G
Sbjct: 6   LLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYG 65

Query: 64  PIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYGEHW 123
            +FSL+ G+RL++VVSS +A +ECFTKNDIV ANRP+   GKY+ YNYTT+  SPYGEHW
Sbjct: 66  SVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHW 125

Query: 124 RNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTFSIV 183
           RNLRR++A+E+ S   LN F+ IREDE+K+L+  L   S ++F  VE++    +L+F++ 
Sbjct: 126 RNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVT 185

Query: 184 MRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEKRIM 243
           MRM+AGK+Y+G D+  DS +++ FR ++ ++  HA + NPGDF+P    ID    EK++ 
Sbjct: 186 MRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 245

Query: 244 KLGQRADELFQGLIDEIR-NQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILAGI 303
           K+ Q  D   Q LI E R N+NN   TMIDHLL LQ +QPEYY+DQ+IKGII  ++LAG 
Sbjct: 246 KISQEMDAFLQRLIHESRINKNN--VTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGT 305

Query: 304 DTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLRLT 363
           DTSAVT+EWA+S LLN+P+ L+KARTEI+  +G  R++ E DL  L+YL  IISET RL 
Sbjct: 306 DTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLC 365

Query: 364 PTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIRPN 423
           P  P+L+PH +S+DCKV+GYD+P+ TI+LVNAWAIHRDP  W++   FKPERH   +   
Sbjct: 366 PAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH-GGVELE 425

Query: 424 TYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKAQP 483
             +L+PFG+GRR+CPG G+AQRVVGLTL +LIQCFEWKR+  + +DM EG G TMPKAQP
Sbjct: 426 PSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQP 485

Query: 484 LIAKCKPHPIMKVILSKNN 502
           L A CKP  I+  ++S+ +
Sbjct: 486 LEALCKPRNILHKVVSETS 499

BLAST of Moc02g12430 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 3.1e-151
Identity = 265/496 (53.43%), Postives = 360/496 (72.58%), Query Frame = 0

Query: 3   FLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKF 62
           + LLS LS + ++ I+L    R R  NLPP PP  +P+IG++H +KHP+HRTF  +S+ +
Sbjct: 5   YSLLS-LSFIITIKILLKITSR-RLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTY 64

Query: 63  GPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYGEH 122
           G IFSL FGSRL+VVVSS S   ECFTKNDI+LANRP   +GKY+ YNYTTLGS+ YG+H
Sbjct: 65  GDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDH 124

Query: 123 WRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEE---FAVVEVEPLFLDLT 182
           WRNLRRI+ ++VLS +RLNSF+G+R DE  RL+ +L  D + E   F  VE+ P   ++T
Sbjct: 125 WRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMT 184

Query: 183 FSIVMRMLAGKKYYGDDMS-EDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGL 242
           F+ +MRM++GK+YYGDD    D  ++++FRE+++++++   + N GDF+P+  ++D   L
Sbjct: 185 FNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNL 244

Query: 243 EKRIMKLGQRADELFQGLIDEIR--NQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQD 302
           EKR  ++ +R++   +GLI+E R  N ++ G TMIDHLL L  +QPEYYSD +IKG+IQ 
Sbjct: 245 EKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQG 304

Query: 303 IILAGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIIS 362
           ++LAG DTSAVTIEW +S LLN+P+VLKKA+ E+D  IG+ ++V+E DLS L YLQ IIS
Sbjct: 305 MLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIIS 364

Query: 363 ETLRLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHT 422
           ETLRL P  P+L+PH +SEDC +  +++P+DTI+L N W IHRDP  W DA SFKPER  
Sbjct: 365 ETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFE 424

Query: 423 NAIRPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTT 482
                N  +++ FG+GRRACPG+ +AQR VG T+  LIQCFEW+R S   +DM EG+G T
Sbjct: 425 KEEEVN--KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGIT 484

Query: 483 MPKAQPLIAKCKPHPI 493
           MP   PL A CK  PI
Sbjct: 485 MPMKIPLRAMCKALPI 495

BLAST of Moc02g12430 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 531.6 bits (1368), Expect = 9.8e-150
Identity = 256/502 (51.00%), Postives = 365/502 (72.71%), Query Frame = 0

Query: 1   MEFL-LLSILSSLFSLLIVLSFLPRPRR-GNLPPSPPFALPLIGHIHLVKHPVHRTFHKI 60
           ME L LLS      +L  + + + R R+  NLPP PP +LP+IG++H +K P+HRTF  +
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60

Query: 61  SKKFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSP 120
           S+K+G +FSL FGSRL+VVVSS+S  Q+CFTKND+VLANRP   SGKY+ YNYTTLGS+ 
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 121 YGEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDL 180
           YGEHWRNLRRI+AL+VLS  R+NSF GIR DE +RL+ RL  DS   FA +E+     D+
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180

Query: 181 TFSIVMRMLAGKKYYGDDM-SEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSG 240
           TF+ +MRM++GK+YYG+D  + D +++ +FR++V+++L  + + N  DF+P+   +D   
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240

Query: 241 LEKRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDI 300
           LEKR+  +  + D   +GLI+E R +    NTMIDHLL LQ++QPEYY+DQ+IKG+   +
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 300

Query: 301 ILAGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISE 360
           +LAG D+SAVT+EW++S+LLN+P+VLKK + E+D  +GQ+R+V+E+DL  L+YL+ +I+E
Sbjct: 301 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 360

Query: 361 TLRLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTN 420
           TLRL    P+L+PH  S++C + GY +P+DTIVL+NAWAIHRDP LW +A +FKPER   
Sbjct: 361 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK 420

Query: 421 AIRPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTM 480
             +    +L+ FG+GRRACPG G+A R + +TLA LIQCF+WK ++   +D+ E +G T+
Sbjct: 421 --KGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTL 480

Query: 481 PKAQPLIAKCKPHPIMKVILSK 500
            K  PL A CK  P++  +  +
Sbjct: 481 TKLVPLKAMCKSRPVINKVFKQ 499

BLAST of Moc02g12430 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-148
Identity = 263/499 (52.71%), Postives = 356/499 (71.34%), Query Frame = 0

Query: 8   ILSSLFSLL-IVLSF-LPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKF--- 67
           +L S+FSL+ +++SF   +P++ NLPPSPP  LP+IGH+ L+K P+HRT    S+     
Sbjct: 9   VLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHN 68

Query: 68  --GPIFSLRFGSRLIVVVSSSS-AVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPY 127
             G + SLR GSRL+ VVSS   A +ECF KND+VLANRP +  GK++ YN T + ++PY
Sbjct: 69  DGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPY 128

Query: 128 GEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLT 187
           G+HWRNLRR+  +E+ ST RLN F+ +R DE++RL+ RL   +  +  VVE++P+ +DLT
Sbjct: 129 GDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLT 188

Query: 188 FSIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLE 247
           F+ +MRM+ GK+YYG++ + D  ++++ R++V  + A+  S N  D++P+  L   S  E
Sbjct: 189 FNNIMRMMTGKRYYGEE-TTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYE 248

Query: 248 KRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIIL 307
            R+ KLG+  D+  QGLID+ R Q   G TMIDHLL LQ +  EYY+DQ+IKGII  +++
Sbjct: 249 NRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVI 308

Query: 308 AGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETL 367
           AG +TSAVT+EWALS+LLN+PDV+ KAR EID  +G +R++ EADLS L YL+ I+ ETL
Sbjct: 309 AGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETL 368

Query: 368 RLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI 427
           RL P  P+L+PH ASEDCK+  YD+PR T +LVNAWAIHRDPN W+D  SFKPER     
Sbjct: 369 RLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK-- 428

Query: 428 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 487
                +LL FG+GRRACPG G+AQR+VGL L SLIQCFEW+RV +  VDM EG G T+PK
Sbjct: 429 EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPK 488

Query: 488 AQPLIAKCKPHPIMKVILS 499
           A PL A CK  P +  I+S
Sbjct: 489 AIPLKAICKARPFLHKIIS 502

BLAST of Moc02g12430 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 9.2e-148
Identity = 262/496 (52.82%), Postives = 347/496 (69.96%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLS--FLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKI 60
           M    LS++ SLF L+I L   F    +  NLPP P   LP+IG++H +K P+H TFH +
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTL 60

Query: 61  SKKFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSP 120
           S+K+G IFSL FGSRL+VVVSS +  QECFTKNDIVLANRP   +GKY+ YN TT+  SP
Sbjct: 61  SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120

Query: 121 YGEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDL 180
           YG+HWRNLRRI ++E+LS+ RLNSF+ IR DEI RL+ +L   S   F  VE+ P+F ++
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEM 180

Query: 181 TFSIVMRMLAGKKYYGDDMS-EDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSG 240
           TF+ +MRM++GK+YYG+D    D  ++R FR ++ ++++   + N GDF+      D  G
Sbjct: 181 TFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG 240

Query: 241 LEKRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDI 300
           LEKR+ K+ +R D   QGLIDE R      NTMIDHLL  Q +QPEYY+DQ+IKG++  +
Sbjct: 241 LEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300

Query: 301 ILAGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISE 360
           +LAG DTS+VTIEWA+S+LLN+P+++KKA+ E+D  IG +R V+E D+S L YLQ I+ E
Sbjct: 301 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 360

Query: 361 TLRLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTN 420
           TLRL    P+L+PH +SED  + GY+IP++TI++VNAW IHRDPNLW D   FKPER   
Sbjct: 361 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 420

Query: 421 AIRPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTM 480
               N  +LL FG+GRRACPG  ++QR  GLTL  LIQCFEWKR+    +DM E +G T 
Sbjct: 421 EGEVN--KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITA 480

Query: 481 PKAQPLIAKCK-PHPI 493
            K   L A CK  HP+
Sbjct: 481 GKKTSLNAMCKVRHPL 493

BLAST of Moc02g12430 vs. ExPASy TrEMBL
Match: A0A6J1D797 (isoflavone 3'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017936 PE=3 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 7.0e-292
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK
Sbjct: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
           KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG
Sbjct: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF
Sbjct: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK
Sbjct: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA
Sbjct: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR
Sbjct: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420
           LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR
Sbjct: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420

Query: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480
           PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA
Sbjct: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480

Query: 481 QPLIAKCKPHPIMKVILSKNN 502
           QPLIAKCKPHPIMKVILSKNN
Sbjct: 481 QPLIAKCKPHPIMKVILSKNN 501

BLAST of Moc02g12430 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 5.1e-218
Identity = 365/498 (73.29%), Postives = 434/498 (87.15%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           M+ LLLSI  SLFSL I   FL RP R NLPPSP F LPLIGH+HLVKHPVHRT HK+S 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
            +G +FSLR+GSRL+VVVSS SAVQECFTKNDIVLANRP++KSGK+LAYN+TTL  SPYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           EHWRNLRRIS LE+ STSRLN F+GIREDE+K L+ +L GDSL+EF  VE++ + LD TF
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           +IV+RM+AGKKYYG+D+  D  QS+KFRE+V +ILA+  + N GDF+P+WN IDP+G EK
Sbjct: 261 NIVVRMIAGKKYYGEDL-RDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEK 320

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           RIMK+G+R+DEL Q L+DEIRNQ +GGNTMIDHLL LQNTQPEY+SDQ+IKG+ Q ++LA
Sbjct: 321 RIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLA 380

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDT+A+T+EWALS LLNNP+VLKKAR EID+ IG++R+V+E DLSSLSYLQG+I+ETLR
Sbjct: 381 GIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLR 440

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI- 420
           L P  P+L+PHCASEDCK+EGYDIPRDTI+L+NAWAIHRD N+WEDA SFKPERH N + 
Sbjct: 441 LNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG 500

Query: 421 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 480
             ++Y+LLPFG+GRRACPGMGMAQRVVGLTLASLIQCFEW+RV+SSLVDMTEGEG TMPK
Sbjct: 501 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 560

Query: 481 AQPLIAKCKPHPIMKVIL 498
           AQPL+AKCKP PI+K IL
Sbjct: 561 AQPLVAKCKPRPILKAIL 577

BLAST of Moc02g12430 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.7e-213
Identity = 359/499 (71.94%), Postives = 428/499 (85.77%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           M+ L LSI  SLFSLL+  SFL R RR NLPPSP F+LP++GH+HLVKHPVH+TFH++S 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
           K+G +FSLRFGSR++VVVSS SAVQECFTKNDIVLANRP++KSGK LAYN+TT+  +PYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           EHWRNLRRI  LE+ ST+RLN F+GIREDE++ LM +LC +S EEF  VE+EP+  DLTF
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           +IVMRM+AGKKYY      + +QS+KFRE+VTQ+LAH  + NPGDFIP+WN IDP+G EK
Sbjct: 181 NIVMRMVAGKKYY------EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEK 240

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           RI+KLGQR+DEL Q LIDEIRNQ  G NTMIDHLL LQ  +PEYYSDQ+IKG+IQ I+LA
Sbjct: 241 RIIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLA 300

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDT+AVT+EWALS LLNNP+VL+KA+ EID  +GQER+VNEADLSSL YLQGII ETLR
Sbjct: 301 GIDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLR 360

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420
           L P  P+LIPHCAS DCK+ GYD+PRDTI+L+N WAIHRDPNLWEDA +FKPERH N I 
Sbjct: 361 LNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIG 420

Query: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480
            ++Y+LLPFG GRR+CPG+GMAQRVVGLTLASLIQ FEW+R+S+SLVDMTEG+G TMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480

Query: 481 QPLIAKCKPHPIMKVILSK 500
           Q L+AKCKP PIMK+IL++
Sbjct: 481 QHLVAKCKPRPIMKLILNE 493

BLAST of Moc02g12430 vs. ExPASy TrEMBL
Match: A0A6J1D8L6 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 3.2e-204
Identity = 348/499 (69.74%), Postives = 413/499 (82.77%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISK 60
           M+ LLLSI  SL  LL  L F P  RR NLPPSP F+LP+IGH+HLVKH VHRT H +S 
Sbjct: 1   MDILLLSIFLSL--LLAFLLFRPH-RRNNLPPSPLFSLPIIGHLHLVKHSVHRTLHNLSH 60

Query: 61  KFGPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 120
            +G +FSLRFGSRL+VVVSS S VQECFTKNDI+LANRP++ + K+LAYN TT+  +PYG
Sbjct: 61  NYGRVFSLRFGSRLVVVVSSPSVVQECFTKNDIILANRPLLDTAKHLAYNQTTMAVAPYG 120

Query: 121 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 180
           +HWR+LRRI ALE+ ST+R+N F+GIRE E+KRLM +LCG S  EF VVE   + LDL F
Sbjct: 121 DHWRHLRRIGALEIFSTTRMNLFLGIREGEVKRLMRKLCGSSFVEFTVVEPGSMILDLMF 180

Query: 181 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 240
           +I+MR++AGKKYY DDMS D + SRKFRE V + +AH  + NPGDFIP+WN IDP+GLEK
Sbjct: 181 NIIMRIVAGKKYYDDDMSNDGK-SRKFREAVKETMAHGGATNPGDFIPIWNWIDPTGLEK 240

Query: 241 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 300
           ++MKLG   DEL Q L+DEIRNQ N GNTMI+HLL LQ ++PE +SD +IKG++  I++A
Sbjct: 241 KMMKLGHTMDELLQELLDEIRNQKNEGNTMIEHLLCLQKSEPENHSDLIIKGLVHVILIA 300

Query: 301 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 360
           GIDT  VT+EWALSHLLNNP+VL+KA+ EID  IGQERMVNEADLSSLSY+QGII ETLR
Sbjct: 301 GIDTIGVTLEWALSHLLNNPEVLEKAKAEIDNSIGQERMVNEADLSSLSYVQGIIFETLR 360

Query: 361 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 420
           LTP  P+L+ HC SEDCK+ GYDIPRD IVL+NAWAIHRDP LWEDA SF+PERHTNAI 
Sbjct: 361 LTPAAPLLVAHCPSEDCKIGGYDIPRDAIVLINAWAIHRDPELWEDATSFRPERHTNAIG 420

Query: 421 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKA 480
            ++Y+LLPFG+GRRACPGMGMAQRVVGLTLASLIQCFEWKR SS LVDMTEG G TMPKA
Sbjct: 421 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWKRASSLLVDMTEGGGLTMPKA 480

Query: 481 QPLIAKCKPHPIMKVILSK 500
           QPL+AKC+P PIM+ IL++
Sbjct: 481 QPLVAKCRPRPIMRAILNE 495

BLAST of Moc02g12430 vs. ExPASy TrEMBL
Match: A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 9.4e-204
Identity = 346/503 (68.79%), Postives = 420/503 (83.50%), Query Frame = 0

Query: 5   LLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKFGP 64
           LL  L SL SLL+  SFL +P R NLPP+P F LP IGH+HL+KHPVH+TF  +S+K+G 
Sbjct: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65

Query: 65  IFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYGEHWR 124
           +FSL+FGSRL+V+VSS SA+QECFTKNDI+LANRP + SGKYLAYN TT+  S YGEHWR
Sbjct: 66  VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGEHWR 125

Query: 125 NLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSL--EEFAVVEVEPLFLDLTFSI 184
           NLRRIS LE+ ST+RLNSF   RE+E+KRL+ +L G+    +EF VVE+EP+ LDLTF+I
Sbjct: 126 NLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNI 185

Query: 185 VMRMLAGKKY---YGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLE 244
           VMRM+ GKK+     +++ ED    ++F+E+VTQI+AH  S NPGDFIP+ N IDPSG  
Sbjct: 186 VMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYT 245

Query: 245 KRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIIL 304
           KRIMK+G++ DE+ QGL+DEIRN+ + GNTMI HLL LQ T PEYYSDQ+IKG++QDI+L
Sbjct: 246 KRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDILL 305

Query: 305 AGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETL 364
           AGIDTSAVT++WALSHLLNNP VL+KA+ EID  IGQERMVNEADLSSLSYLQGIISETL
Sbjct: 306 AGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGIISETL 365

Query: 365 RLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI 424
           RL+P GP+L+PHC+SEDCK+ GYD+PR+TIVL+NAWAIHRDPNLWEDA SFKPERH NA 
Sbjct: 366 RLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAA 425

Query: 425 R-PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMP 484
              N+Y+LLPFG+GRRACPGM MAQRV+GLTLASL+QCFEWKR+S  LVDM EGEG TMP
Sbjct: 426 GFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMP 485

Query: 485 KAQPLIAKCKPHPIMKVILSKNN 502
           K +PL+AKC+P  IM+ +LS+ N
Sbjct: 486 KVEPLVAKCRPRFIMEAVLSEKN 508

BLAST of Moc02g12430 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 536.2 bits (1380), Expect = 2.8e-152
Identity = 264/499 (52.91%), Postives = 366/499 (73.35%), Query Frame = 0

Query: 4   LLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKF- 63
           L+ SIL  + SL+ ++  L   R+ NLPPSP ++LP+IGH+ L+K P+HRTF  +S+   
Sbjct: 6   LIFSILFVVLSLIYLIGKL--KRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65

Query: 64  -GPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYGE 123
             PIFSLR G+RL+ V SS S  +ECFTKND+VLANRP     K++AY+YTT+ ++ YG+
Sbjct: 66  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125

Query: 124 HWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTFS 183
           HWRNLRRI ++E+ S  RLNSF+ IR+DEI+RL+ RL  +  +EF  V+++ +  DLTF+
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 185

Query: 184 IVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEKR 243
            ++RM+AGK+YYGD + ED  ++++ R+++  ++A A + N  D++P+  L+  S  E R
Sbjct: 186 NILRMVAGKRYYGDGV-EDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETR 245

Query: 244 IMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILAG 303
           + KL  R DE  QGL+DE R     GNTMIDHLL LQ +QP+Y++D++IKG +  +ILAG
Sbjct: 246 VKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 305

Query: 304 IDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLRL 363
            DTSAVT+EWALS++LN+PDVL KAR EID  IG +R+++E+D+S+L YLQ I+SETLRL
Sbjct: 306 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 365

Query: 364 TPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIRP 423
            P  P+L+PH ASEDCKV GYD+PR TI+L N WAIHRDP LW+D  SFKPER       
Sbjct: 366 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK--EG 425

Query: 424 NTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPKAQ 483
              +L+PFG+GRRACPG G+A R++ LTL SLIQC EW+++    VDM+EG+G TMPKA+
Sbjct: 426 EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAK 485

Query: 484 PLIAKCKPHPIMKVILSKN 501
           PL A C+  P +  I +++
Sbjct: 486 PLEAMCRARPSVVKIFNES 496

BLAST of Moc02g12430 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 528.1 bits (1359), Expect = 7.7e-150
Identity = 263/499 (52.71%), Postives = 356/499 (71.34%), Query Frame = 0

Query: 8   ILSSLFSLL-IVLSF-LPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKF--- 67
           +L S+FSL+ +++SF   +P++ NLPPSPP  LP+IGH+ L+K P+HRT    S+     
Sbjct: 9   VLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHN 68

Query: 68  --GPIFSLRFGSRLIVVVSSSS-AVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPY 127
             G + SLR GSRL+ VVSS   A +ECF KND+VLANRP +  GK++ YN T + ++PY
Sbjct: 69  DGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPY 128

Query: 128 GEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLT 187
           G+HWRNLRR+  +E+ ST RLN F+ +R DE++RL+ RL   +  +  VVE++P+ +DLT
Sbjct: 129 GDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLT 188

Query: 188 FSIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLE 247
           F+ +MRM+ GK+YYG++ + D  ++++ R++V  + A+  S N  D++P+  L   S  E
Sbjct: 189 FNNIMRMMTGKRYYGEE-TTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYE 248

Query: 248 KRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIIL 307
            R+ KLG+  D+  QGLID+ R Q   G TMIDHLL LQ +  EYY+DQ+IKGII  +++
Sbjct: 249 NRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVI 308

Query: 308 AGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETL 367
           AG +TSAVT+EWALS+LLN+PDV+ KAR EID  +G +R++ EADLS L YL+ I+ ETL
Sbjct: 309 AGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETL 368

Query: 368 RLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI 427
           RL P  P+L+PH ASEDCK+  YD+PR T +LVNAWAIHRDPN W+D  SFKPER     
Sbjct: 369 RLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK-- 428

Query: 428 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 487
                +LL FG+GRRACPG G+AQR+VGL L SLIQCFEW+RV +  VDM EG G T+PK
Sbjct: 429 EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPK 488

Query: 488 AQPLIAKCKPHPIMKVILS 499
           A PL A CK  P +  I+S
Sbjct: 489 AIPLKAICKARPFLHKIIS 502

BLAST of Moc02g12430 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 521.9 bits (1343), Expect = 5.5e-148
Identity = 256/494 (51.82%), Postives = 354/494 (71.66%), Query Frame = 0

Query: 2   EFLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKK 61
           + LLLS L    S+ ++L+     R+ NLPPSP + LP+IGH+HL+K PVHRTFH ISK 
Sbjct: 5   QILLLSFLFLTISIKLLLT--KSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKS 64

Query: 62  FG--PIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPY 121
            G  PIF LR G+RL+ V+SS S  +ECFTKND+VLANRP +   K++ YN+T + ++ Y
Sbjct: 65  LGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASY 124

Query: 122 GEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLT 181
           G+HWRNLRRI+A+E+ S+ R+++F  IR+DEI+RL+  L  DSL  F  VE++ L  +L 
Sbjct: 125 GDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLA 184

Query: 182 FSIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLE 241
           F+ ++ M+AGK+YYG   +ED+ +++  RE++ +I+A A S N  D++P  N +  +  E
Sbjct: 185 FNNIIMMVAGKRYYGTG-TEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFE 244

Query: 242 KRIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIIL 301
            +   LG R D + Q L+DE R +   G T+IDHLL  Q T+PEYY+D +IKGII  ++L
Sbjct: 245 NQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVL 304

Query: 302 AGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETL 361
           AG DTS+VT+EWA+S+LLN+P++L+KAR EID  IG +R+V E+D+ +L YLQ I+SETL
Sbjct: 305 AGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETL 364

Query: 362 RLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAI 421
           RL P  P+L+PH +S++CKV GYD+PR T++L N WA+HRDP LWE+ + FKPER     
Sbjct: 365 RLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEK-- 424

Query: 422 RPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTTMPK 481
                +L+PFG+GRRACPG  + +R+V L L  LIQ FEW+RV + LVDMTEGEG TMPK
Sbjct: 425 EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPK 484

Query: 482 AQPLIAKCKPHPIM 494
           A PL A CK   I+
Sbjct: 485 ATPLRAMCKARAIV 491

BLAST of Moc02g12430 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 521.5 bits (1342), Expect = 7.2e-148
Identity = 261/498 (52.41%), Postives = 351/498 (70.48%), Query Frame = 0

Query: 3   FLLLSILSSLFSLLIVLSFLPRPRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKISKKF 62
           +L+LS+      L + L F PRPR+ NLPPSP    P+IGH+HL+K P+HR F  +S+  
Sbjct: 12  YLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESL 71

Query: 63  --GPIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSSPYG 122
               IFSL  GSRL+ VVSS +  +ECFTKND+VLANRP    GK++ YN TT+  + YG
Sbjct: 72  NNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYG 131

Query: 123 EHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLDLTF 182
           + WRNLRRI  +E+ S+ RLNSFV IR+DEI+RL+  L  +S   F  VE++PLF+ LT 
Sbjct: 132 DSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTI 191

Query: 183 SIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSGLEK 242
           + ++RM+AGK++YGD  +E+  +++  R+++ +++    + N  D+ P+   +  +  EK
Sbjct: 192 NNIIRMVAGKRFYGDG-TENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEK 251

Query: 243 RIMKLGQRADELFQGLIDEIRNQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQDIILA 302
            + KL  R DE  Q L++E R +   GNTMIDHLL LQ TQP+YY+D +IKGII  +ILA
Sbjct: 252 HVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILA 311

Query: 303 GIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIISETLR 362
           G DTSA T+EWA+S+LLN+P+VL+KA+TEID  IG +R+V E D+  L YLQ I+SETLR
Sbjct: 312 GTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLR 371

Query: 363 LTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHTNAIR 422
           L P  P+L+PH ASEDC V+GYD+PR TI+LVNAWAIHRDP LWE+ + FKPER     +
Sbjct: 372 LYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK--K 431

Query: 423 PNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGE-GTTMPK 482
               +L+PFG+GRR+CPG G+AQR+V L L SL+QCFEW+RV    +DM E E GTTM K
Sbjct: 432 GEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRK 491

Query: 483 AQPLIAKCKPHPIMKVIL 498
           A  L A CK  PI+  +L
Sbjct: 492 ATSLQAMCKARPIVHKVL 504

BLAST of Moc02g12430 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 516.5 bits (1329), Expect = 2.3e-146
Identity = 269/501 (53.69%), Postives = 351/501 (70.06%), Query Frame = 0

Query: 1   MEFLLLSILSSLFSLLIVLSFLPR-PRRGNLPPSPPFALPLIGHIHLVKHPVHRTFHKIS 60
           ME L+L I +  F +L ++  + R  R+ NLPPSP +ALP+IGH+ L+K P+HR F  +S
Sbjct: 1   MEALML-IFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 61  KKFG--PIFSLRFGSRLIVVVSSSSAVQECFTKNDIVLANRPIMKSGKYLAYNYTTLGSS 120
           +  G  PI SLR G+RL+ VVSS S  +ECFTKND++LANR    S K+++Y  +T+ S+
Sbjct: 61  QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120

Query: 121 PYGEHWRNLRRISALEVLSTSRLNSFVGIREDEIKRLMHRLCGDSLEEFAVVEVEPLFLD 180
            Y EHWRNLRRI ALE+ S  RLNSF  IR DEI+RL+ RL  +S   F  VE++ +F D
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSD 180

Query: 181 LTFSIVMRMLAGKKYYGDDMSEDSRQSRKFREVVTQILAHAVSWNPGDFIPMWNLIDPSG 240
           LTF+ ++RMLAGK YYGD   ED  ++++ R ++ + ++ +   N  D+IP+   I  S 
Sbjct: 181 LTFNNIIRMLAGKCYYGDG-KEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS- 240

Query: 241 LEKRIMKLGQRADELFQGLIDEIR-NQNNGGNTMIDHLLGLQNTQPEYYSDQVIKGIIQD 300
            E RI KL  R DE  QGL+DE R  +    NTM+DHLL LQ TQPEYY D++IKG +  
Sbjct: 241 -ETRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLS 300

Query: 301 IILAGIDTSAVTIEWALSHLLNNPDVLKKARTEIDYCIGQERMVNEADLSSLSYLQGIIS 360
           +I  G DT+AVT+EWALS LLNNP+VL KAR EID  IG +R++ E+D+ +L YLQ I+S
Sbjct: 301 LIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVS 360

Query: 361 ETLRLTPTGPILIPHCASEDCKVEGYDIPRDTIVLVNAWAIHRDPNLWEDAKSFKPERHT 420
           ETLRL P  P+L+PH AS+DCKV GYD+PR T++L NAWAIHRDP LW+D  SFKPER  
Sbjct: 361 ETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFE 420

Query: 421 NAIRPNTYELLPFGVGRRACPGMGMAQRVVGLTLASLIQCFEWKRVSSSLVDMTEGEGTT 480
                   +L+PFG+GRRACPG G+AQR+V L+L SLIQCFEW+R+    VDMTEG G T
Sbjct: 421 K--EGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLT 480

Query: 481 MPKAQPLIAKCKPHPIMKVIL 498
           MPKA+PL A C+    +  IL
Sbjct: 481 MPKARPLEAMCRARDFVGKIL 495