Moc02g12320 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g12320
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCytochrome P450
Locationchr2: 8940693 .. 8941334 (-)
RNA-Seq ExpressionMoc02g12320
SyntenyMoc02g12320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGTTTTGCTGCGTGCGGGAACAGACACATCATCCGTGACTTTGAATTGGGCAATGACCCAATTGCTGAACAATCCAGAGGTGCTGGCAAAAGCCAAAGCTGAGTTGGACGCAAACGTCGGTCAAGATCGATTCGTCGACGAATCCGATCTCGCCAACCTCAACTTCCTTCAAGCCATTGTATCCGAGACCCTGCGCTTGCAACCGGCTGCTCCGATGCTGCTCTCACATTACTCCTCCGAGGACTGCACGGTTGCTGGCTACGACATTCCACGTGGGACTACGTTGTTGGTCAATGCATGGGCGATTCACCGGGACCCGAAGCTGTGGGACGACCCGACGAGGTTTCGGCCGGAGAGGTTTTTGGGGTCAGCAGCAAATGAGTCGCAGTGCAACAAAGTTATTGCATTTGGGCTTGGAAGGAGGGCCTGTCCTGGAGAAACTATGGCTCTACGTTTTGTGGGACTCACTTTGGGGCTGCTCATTCAGTGTTATGAGTGGAAAAAGGGTGGGGAAGAAGAGATTGACATGGGCGAAGGAGGAGGCATTACAATTCACAAAGCTAAGCCTTTGGAGGTTATGTGTAAACCTCGCCCGGTTATGGATAAACTTCAGTTCAATGCTCTGCATAAAATTTGA

mRNA sequence

ATGCAGGTTTTGCTGCGTGCGGGAACAGACACATCATCCGTGACTTTGAATTGGGCAATGACCCAATTGCTGAACAATCCAGAGGTGCTGGCAAAAGCCAAAGCTGAGTTGGACGCAAACGTCGGTCAAGATCGATTCGTCGACGAATCCGATCTCGCCAACCTCAACTTCCTTCAAGCCATTGTATCCGAGACCCTGCGCTTGCAACCGGCTGCTCCGATGCTGCTCTCACATTACTCCTCCGAGGACTGCACGGTTGCTGGCTACGACATTCCACGTGGGACTACGTTGTTGGTCAATGCATGGGCGATTCACCGGGACCCGAAGCTGTGGGACGACCCGACGAGGTTTCGGCCGGAGAGGTTTTTGGGGTCAGCAGCAAATGAGTCGCAGTGCAACAAAGTTATTGCATTTGGGCTTGGAAGGAGGGCCTGTCCTGGAGAAACTATGGCTCTACGTTTTGTGGGACTCACTTTGGGGCTGCTCATTCAGTGTTATGAGTGGAAAAAGGGTGGGGAAGAAGAGATTGACATGGGCGAAGGAGGAGGCATTACAATTCACAAAGCTAAGCCTTTGGAGGTTATGTGTAAACCTCGCCCGGTTATGGATAAACTTCAGTTCAATGCTCTGCATAAAATTTGA

Coding sequence (CDS)

ATGCAGGTTTTGCTGCGTGCGGGAACAGACACATCATCCGTGACTTTGAATTGGGCAATGACCCAATTGCTGAACAATCCAGAGGTGCTGGCAAAAGCCAAAGCTGAGTTGGACGCAAACGTCGGTCAAGATCGATTCGTCGACGAATCCGATCTCGCCAACCTCAACTTCCTTCAAGCCATTGTATCCGAGACCCTGCGCTTGCAACCGGCTGCTCCGATGCTGCTCTCACATTACTCCTCCGAGGACTGCACGGTTGCTGGCTACGACATTCCACGTGGGACTACGTTGTTGGTCAATGCATGGGCGATTCACCGGGACCCGAAGCTGTGGGACGACCCGACGAGGTTTCGGCCGGAGAGGTTTTTGGGGTCAGCAGCAAATGAGTCGCAGTGCAACAAAGTTATTGCATTTGGGCTTGGAAGGAGGGCCTGTCCTGGAGAAACTATGGCTCTACGTTTTGTGGGACTCACTTTGGGGCTGCTCATTCAGTGTTATGAGTGGAAAAAGGGTGGGGAAGAAGAGATTGACATGGGCGAAGGAGGAGGCATTACAATTCACAAAGCTAAGCCTTTGGAGGTTATGTGTAAACCTCGCCCGGTTATGGATAAACTTCAGTTCAATGCTCTGCATAAAATTTGA

Protein sequence

MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI
Homology
BLAST of Moc02g12320 vs. NCBI nr
Match: XP_022149478.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 440.7 bits (1132), Expect = 7.4e-120
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA
Sbjct: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE
Sbjct: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE
Sbjct: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI 214
           GGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI
Sbjct: 181 GGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI 213

BLAST of Moc02g12320 vs. NCBI nr
Match: AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 367.5 bits (942), Expect = 8.0e-98
Identity = 178/211 (84.36%), Postives = 190/211 (90.05%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           VLLRAGTDTS+VTLNWAM QLLNNPE+LAKAKAELD  +GQDR VDE DL NL++LQAIV
Sbjct: 293 VLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIV 352

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
           SETLRL PAAPMLLSHYSS DCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPT FRPERF
Sbjct: 353 SETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERF 412

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGG 182
           LG AANE Q  K+IAFGLGRR+CPG+TMALRFVGLTLGLLIQCY+WKK G+E++DMGEGG
Sbjct: 413 LG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGG 472

Query: 183 GITIHKAKPLEVMCKPRPVMDKLQFNALHKI 214
           GITIHKAKPLE MCK RP M KL  NAL KI
Sbjct: 473 GITIHKAKPLEAMCKARPAMYKLLLNALDKI 502

BLAST of Moc02g12320 vs. NCBI nr
Match: XP_022149456.1 (cytochrome P450 81E8-like isoform X1 [Momordica charantia])

HSP 1 Score: 356.7 bits (914), Expect = 1.4e-94
Identity = 172/209 (82.30%), Postives = 181/209 (86.60%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           MQVLLRAGTD++SVT+NWAMTQLLNNPEVL KAKAELD  VGQDR VDESDLANL +LQA
Sbjct: 307 MQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQA 366

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IV ETLRL P APML++HYSS DCTVAGY IP  T LLVNAWAIHRDPKLWDDPT FRPE
Sbjct: 367 IVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPE 426

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RFLGSAANE   NK+IAFGLGRRACPG TM  RFVGLTL L+IQCYEWKK GEE IDMGE
Sbjct: 427 RFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGE 486

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKLQFNA 210
           GGGITI KAKPLEVMCK RPVMDK+   A
Sbjct: 487 GGGITILKAKPLEVMCKARPVMDKVHVKA 515

BLAST of Moc02g12320 vs. NCBI nr
Match: XP_022149457.1 (cytochrome P450 81E8-like isoform X2 [Momordica charantia])

HSP 1 Score: 353.2 bits (905), Expect = 1.6e-93
Identity = 170/207 (82.13%), Postives = 179/207 (86.47%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           VLLRAGTD++SVT+NWAMTQLLNNPEVL KAKAELD  VGQDR VDESDLANL +LQAIV
Sbjct: 306 VLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIV 365

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
            ETLRL P APML++HYSS DCTVAGY IP  T LLVNAWAIHRDPKLWDDPT FRPERF
Sbjct: 366 FETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERF 425

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGG 182
           LGSAANE   NK+IAFGLGRRACPG TM  RFVGLTL L+IQCYEWKK GEE IDMGEGG
Sbjct: 426 LGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGG 485

Query: 183 GITIHKAKPLEVMCKPRPVMDKLQFNA 210
           GITI KAKPLEVMCK RPVMDK+   A
Sbjct: 486 GITILKAKPLEVMCKARPVMDKVHVKA 512

BLAST of Moc02g12320 vs. NCBI nr
Match: XP_038900755.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 334.7 bits (857), Expect = 5.7e-88
Identity = 157/209 (75.12%), Postives = 181/209 (86.60%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           VLLRAG DTSSVTL+WAMTQLLN+ ++LAKAKAE+D  +GQDR ++E+DL+NLN+LQAI+
Sbjct: 48  VLLRAGVDTSSVTLDWAMTQLLNHLDILAKAKAEIDTKIGQDRPLEEADLSNLNYLQAII 107

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
           SET RL P APMLL+HYSS+DCT+AGY+IPRGTTLLVNAWAIH DPKLWDDPT FRPERF
Sbjct: 108 SETFRLHPPAPMLLTHYSSDDCTIAGYNIPRGTTLLVNAWAIHIDPKLWDDPTSFRPERF 167

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWK-KGGEEEIDMGEG 182
           LG   N  Q NK+I FG+GRRACPGETMALRF+GLTLGLLIQCYEWK  GG + +DM EG
Sbjct: 168 LG-VVNGLQSNKIIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKTAGGYKNVDMTEG 227

Query: 183 GGITIHKAKPLEVMCKPRPVMDKLQFNAL 211
           GGIT+HK KPLE MCK RP+MDK+  N L
Sbjct: 228 GGITLHKVKPLETMCKARPIMDKILSNGL 255

BLAST of Moc02g12320 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 6.2e-69
Identity = 120/205 (58.54%), Postives = 157/205 (76.59%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           + VLL AGTDTS+VT+ WAM+ LLN+PE L KA+ E++  VG +R ++E DL  L +L  
Sbjct: 293 IMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHN 352

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           I+SET RL PAAPML+ H SS+DC V GYD+P+GT LLVNAWAIHRDP+ WD+PT F+PE
Sbjct: 353 IISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPE 412

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           R  G    E + +K++ FG+GRR+CPG  +A R VGLTLG LIQC+EWK+ GE +IDM E
Sbjct: 413 RHGGV---ELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAE 472

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKL 206
           G G+T+ KA+PLE +CKPR ++ K+
Sbjct: 473 GSGLTMPKAQPLEALCKPRNILHKV 494

BLAST of Moc02g12320 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-66
Identity = 115/203 (56.65%), Postives = 153/203 (75.37%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           +++ AGT+TS+VTL WA++ LLN+P+V++KA+ E+D  VG DR ++E+DL+ L +L+ IV
Sbjct: 302 IMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIV 361

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
            ETLRL PA P+L+ H +SEDC +  YD+PRGTTLLVNAWAIHRDP  WDDP  F+PERF
Sbjct: 362 LETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF 421

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGG 182
                 E +  K++AFGLGRRACPG  +A R VGL LG LIQC+EW++ G  E+DM EG 
Sbjct: 422 ----EKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGV 481

Query: 183 GITIHKAKPLEVMCKPRPVMDKL 206
           G T+ KA PL+ +CK RP + K+
Sbjct: 482 GNTVPKAIPLKAICKARPFLHKI 500

BLAST of Moc02g12320 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.2e-66
Identity = 121/199 (60.80%), Postives = 150/199 (75.38%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           M V+L AGTDTSSVT+ WAM+ LLN+PE++ KAK ELD ++G DR VDE D++ L +LQ+
Sbjct: 296 MVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQS 355

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IV ETLRL  AAP+L+ H SSED ++ GY+IP+ T L+VNAW IHRDP LW DPT F+PE
Sbjct: 356 IVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPE 415

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E + NK+++FGLGRRACPGE ++ R  GLTLGLLIQC+EWK+ GEE+IDM E
Sbjct: 416 RF----EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVE 475

Query: 181 GGGITIHKAKPLEVMCKPR 200
             GIT  K   L  MCK R
Sbjct: 476 AKGITAGKKTSLNAMCKVR 490

BLAST of Moc02g12320 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-65
Identity = 115/201 (57.21%), Postives = 150/201 (74.63%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           +Q +L AGTDTS+VT+ W M++LLN+PEVL KAK ELD  +G+++ VDE DL+ L +LQ 
Sbjct: 299 IQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQN 358

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           I+SETLRL P AP+LL HYSSEDCT+  +++P+ T +L N W IHRDPK W+D   F+PE
Sbjct: 359 IISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPE 418

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E + NKV+AFGLGRRACPG ++A R VG T+GLLIQC+EW++  EE++DM E
Sbjct: 419 RF----EKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMME 478

Query: 181 GGGITIHKAKPLEVMCKPRPV 202
           G GIT+    PL  MCK  P+
Sbjct: 479 GKGITMPMKIPLRAMCKALPI 495

BLAST of Moc02g12320 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 6.0e-64
Identity = 115/204 (56.37%), Postives = 150/204 (73.53%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           V++ AGTDTS+ TL WAM+ LLN+PEVL KAK E+D  +G DR V+E D+  L +LQ IV
Sbjct: 304 VMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIV 363

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
           SETLRL P APMLL H +SEDC V GYD+PRGT +LVNAWAIHRDPKLW++P +F+PERF
Sbjct: 364 SETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERF 423

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEG- 182
                 + +  K++ FG+GRR+CPG  +A R V L LG L+QC+EW++  E+ +DM E  
Sbjct: 424 ----EKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESE 483

Query: 183 GGITIHKAKPLEVMCKPRPVMDKL 206
            G T+ KA  L+ MCK RP++ K+
Sbjct: 484 KGTTMRKATSLQAMCKARPIVHKV 503

BLAST of Moc02g12320 vs. ExPASy TrEMBL
Match: A0A6J1D6W7 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111017895 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 3.6e-120
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA
Sbjct: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE
Sbjct: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE
Sbjct: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI 214
           GGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI
Sbjct: 181 GGGITIHKAKPLEVMCKPRPVMDKLQFNALHKI 213

BLAST of Moc02g12320 vs. ExPASy TrEMBL
Match: K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.9e-98
Identity = 178/211 (84.36%), Postives = 190/211 (90.05%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           VLLRAGTDTS+VTLNWAM QLLNNPE+LAKAKAELD  +GQDR VDE DL NL++LQAIV
Sbjct: 293 VLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSYLQAIV 352

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
           SETLRL PAAPMLLSHYSS DCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPT FRPERF
Sbjct: 353 SETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSFRPERF 412

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGG 182
           LG AANE Q  K+IAFGLGRR+CPG+TMALRFVGLTLGLLIQCY+WKK G+E++DMGEGG
Sbjct: 413 LG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVDMGEGG 472

Query: 183 GITIHKAKPLEVMCKPRPVMDKLQFNALHKI 214
           GITIHKAKPLE MCK RP M KL  NAL KI
Sbjct: 473 GITIHKAKPLEAMCKARPAMYKLLLNALDKI 502

BLAST of Moc02g12320 vs. ExPASy TrEMBL
Match: A0A6J1D8F1 (cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.8e-95
Identity = 172/209 (82.30%), Postives = 181/209 (86.60%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           MQVLLRAGTD++SVT+NWAMTQLLNNPEVL KAKAELD  VGQDR VDESDLANL +LQA
Sbjct: 307 MQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQA 366

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IV ETLRL P APML++HYSS DCTVAGY IP  T LLVNAWAIHRDPKLWDDPT FRPE
Sbjct: 367 IVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPE 426

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RFLGSAANE   NK+IAFGLGRRACPG TM  RFVGLTL L+IQCYEWKK GEE IDMGE
Sbjct: 427 RFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGE 486

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKLQFNA 210
           GGGITI KAKPLEVMCK RPVMDK+   A
Sbjct: 487 GGGITILKAKPLEVMCKARPVMDKVHVKA 515

BLAST of Moc02g12320 vs. ExPASy TrEMBL
Match: A0A6J1D806 (cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 7.5e-94
Identity = 170/207 (82.13%), Postives = 179/207 (86.47%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           VLLRAGTD++SVT+NWAMTQLLNNPEVL KAKAELD  VGQDR VDESDLANL +LQAIV
Sbjct: 306 VLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIV 365

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
            ETLRL P APML++HYSS DCTVAGY IP  T LLVNAWAIHRDPKLWDDPT FRPERF
Sbjct: 366 FETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERF 425

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGG 182
           LGSAANE   NK+IAFGLGRRACPG TM  RFVGLTL L+IQCYEWKK GEE IDMGEGG
Sbjct: 426 LGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGG 485

Query: 183 GITIHKAKPLEVMCKPRPVMDKLQFNA 210
           GITI KAKPLEVMCK RPVMDK+   A
Sbjct: 486 GITILKAKPLEVMCKARPVMDKVHVKA 512

BLAST of Moc02g12320 vs. ExPASy TrEMBL
Match: A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.9e-87
Identity = 152/208 (73.08%), Postives = 177/208 (85.10%), Query Frame = 0

Query: 3   VLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQAIV 62
           VLLRAG D++SVT+ WAMTQLLNNPEV++KAKAE+D  +G+DR +DE+DLANL++LQAI+
Sbjct: 292 VLLRAGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAII 351

Query: 63  SETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPERF 122
           SET RL PAAPMLL+HYSS DCTVAGYD+PRGT LLVNAWAIHRDPKLWDDP  FRPERF
Sbjct: 352 SETFRLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERF 411

Query: 123 LGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGEGG 182
           LG   NE   NK+I FG+GRRACPGETMALR +GL+LGLLIQCYEWK  G E++DM EGG
Sbjct: 412 LGE-GNELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGG 471

Query: 183 GITIHKAKPLEVMCKPRPVMDKLQFNAL 211
           GITI K +PLE MC+  P+MDK+  N L
Sbjct: 472 GITIQKVQPLETMCRALPIMDKVLSNGL 498

BLAST of Moc02g12320 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 267.7 bits (683), Expect = 8.0e-72
Identity = 127/205 (61.95%), Postives = 157/205 (76.59%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           M  L+ AGTDTS+VTL WA++ +LN+P+VL KA+ E+D  +G DR +DESD++NL +LQ 
Sbjct: 293 MLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQN 352

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSETLRL PAAPMLL H +SEDC VAGYD+PRGT LL N WAIHRDP+LWDDP  F+PE
Sbjct: 353 IVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPE 412

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E +  K++ FGLGRRACPG  +A R + LTLG LIQC EW+K G EE+DM E
Sbjct: 413 RF----EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDMSE 472

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKL 206
           G G+T+ KAKPLE MC+ RP + K+
Sbjct: 473 GKGVTMPKAKPLEAMCRARPSVVKI 492

BLAST of Moc02g12320 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 266.5 bits (680), Expect = 1.8e-71
Identity = 125/205 (60.98%), Postives = 156/205 (76.10%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           M  L+  GTDT++VTL WA++ LLNNPEVL KA+ E+D  +G DR ++ESD+ NL +LQ 
Sbjct: 294 MLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQN 353

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSETLRL PAAPMLL H +S+DC V GYD+PRGT LL NAWAIHRDP LWDDPT F+PE
Sbjct: 354 IVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPE 413

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E +  K++ FGLGRRACPG  +A R V L+LG LIQC+EW++ GEEE+DM E
Sbjct: 414 RF----EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTE 473

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKL 206
           G G+T+ KA+PLE MC+ R  + K+
Sbjct: 474 GPGLTMPKARPLEAMCRARDFVGKI 494

BLAST of Moc02g12320 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 3.4e-70
Identity = 122/205 (59.51%), Postives = 154/205 (75.12%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           M  L+ AGTDTS+VTL WA++ LLNNPEVL K + E+D  +G DR ++ESD+ NL +LQ 
Sbjct: 181 MLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQN 240

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSETLRL PA P+L+ H SSEDC V GYD+P GT LLVN WAIHRDP+LWDDP  F+PE
Sbjct: 241 IVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPE 300

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E + +K++ FGLGRRACPG  +A R V L+LG LIQC+EW++ GEEE+DM E
Sbjct: 301 RF----EKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTE 360

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKL 206
           GGG+T+ +A PL  MC+ R  + K+
Sbjct: 361 GGGLTMPRAIPLVAMCRARAFVGKI 381

BLAST of Moc02g12320 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 3.4e-70
Identity = 122/205 (59.51%), Postives = 154/205 (75.12%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           M  L+ AGTDTS+VTL WA++ LLNNPEVL K + E+D  +G DR ++ESD+ NL +LQ 
Sbjct: 122 MLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQN 181

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSETLRL PA P+L+ H SSEDC V GYD+P GT LLVN WAIHRDP+LWDDP  F+PE
Sbjct: 182 IVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPE 241

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E + +K++ FGLGRRACPG  +A R V L+LG LIQC+EW++ GEEE+DM E
Sbjct: 242 RF----EKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTE 301

Query: 181 GGGITIHKAKPLEVMCKPRPVMDKL 206
           GGG+T+ +A PL  MC+ R  + K+
Sbjct: 302 GGGLTMPRAIPLVAMCRARAFVGKI 322

BLAST of Moc02g12320 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 258.8 bits (660), Expect = 3.7e-69
Identity = 121/206 (58.74%), Postives = 157/206 (76.21%), Query Frame = 0

Query: 1   MQVLLRAGTDTSSVTLNWAMTQLLNNPEVLAKAKAELDANVGQDRFVDESDLANLNFLQA 60
           M  L+ AGTDTS+VTL W ++ LLN+P++L+KA+ E+D  VG +R V+ESDL++L +LQ 
Sbjct: 294 MLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQN 353

Query: 61  IVSETLRLQPAAPMLLSHYSSEDCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTRFRPE 120
           IVSE+LRL PA+P+L+ H +SEDC V GY +PRGT LL NAWAIHRDPK+WDDPT F+PE
Sbjct: 354 IVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPE 413

Query: 121 RFLGSAANESQCNKVIAFGLGRRACPGETMALRFVGLTLGLLIQCYEWKKGGEEEIDMGE 180
           RF      E +  K++ FGLGRRACPG  +A R   LT+G LIQC+EW++ GEEE+DM E
Sbjct: 414 RF----EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTE 473

Query: 181 -GGGITIHKAKPLEVMCKPRPVMDKL 206
            GGG+ + KA PL  MCK RPV+ K+
Sbjct: 474 GGGGVIMPKAIPLVAMCKARPVVGKI 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149478.17.4e-120100.00cytochrome P450 81D1-like [Momordica charantia][more]
AEM42992.18.0e-9884.36cytochrome P450 [Siraitia grosvenorii][more]
XP_022149456.11.4e-9482.30cytochrome P450 81E8-like isoform X1 [Momordica charantia][more]
XP_022149457.11.6e-9382.13cytochrome P450 81E8-like isoform X2 [Momordica charantia][more]
XP_038900755.15.7e-8875.12cytochrome P450 81Q32-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
W8JMU76.2e-6958.54Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q9FG651.7e-6656.65Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
Q6WNQ82.2e-6660.80Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q6WNQ91.1e-6557.21Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q9LHA16.0e-6456.37Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D6W73.6e-120100.00cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111017895 PE=3 SV... [more]
K7NC013.9e-9884.36Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1[more]
A0A6J1D8F16.8e-9582.30cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1D8067.5e-9482.13cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1FUQ98.9e-8773.08cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT4G37370.18.0e-7261.95cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37360.11.8e-7160.98cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT1G66540.13.4e-7059.51Cytochrome P450 superfamily protein [more]
AT1G66540.23.4e-7059.51Cytochrome P450 superfamily protein [more]
AT4G37340.13.7e-6958.74cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 60..71
score: 41.31
coord: 145..156
score: 33.01
coord: 7..24
score: 39.98
coord: 136..145
score: 51.99
IPR001128Cytochrome P450PFAMPF00067p450coord: 3..187
e-value: 9.1E-59
score: 199.4
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 16..42
score: 36.47
coord: 59..77
score: 33.17
coord: 145..168
score: 32.32
coord: 135..145
score: 49.62
coord: 100..124
score: 42.63
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..202
e-value: 1.2E-67
score: 230.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 3..199
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 2..204
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 2..204
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 138..147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g12320.1Moc02g12320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen