Moc02g12310 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g12310
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome P450
Location	chr2: 8932956 .. 8935433 (-)
RNA-Seq Expression	Moc02g12310
Synteny	Moc02g12310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCTCTCTCTGTTTTCGCTCTGCTCCTCTTCGCACTCGCCTCAATCCTTTTTCGGCAGCAGATCAGAGCCCCGCGGCGGAATCTACCGCCGAGCCCGCCGTCTGTTCCGATCATCGGCCACCTCCGCCTCCTCCGCCGCCCCGTCCACCGCAACTTCCAGAAGATCGCCGCCGAATACGGCCCCATTTTCTCTCTCAGATTCGGATCTCGCCTCGCCGTCGTAGTCTCTTCACTCGACACAGCCGAAGAATGCCTAACGAAGAACGACGTCGTCTTCGCCAACCGGCCGCGCCTCATCATCGGCAAGCACCTCGGCTACAACCACACCACCATGGCCGCCGCGCCGTACGGCGCCCACTGGCGGAACCTCCGGCGCCTCACCGCCAGCGAGCTCTTCTCCACGGCCCGCCTCGGCTCCTCTCTGAGCATCCGGAAGGAGGAAATCCGGCGGGTGCTGCTGAAGCTCCGGGCCGGGTCGGGGTCCGGGTCGGGCGGGGACTTCGCGAAGGCGGAGTTGAAATCGATGTTCTCGGAGGTGACGTTCAACGTGGTGATGAGGATGGTGGCCGGAAAACGGTACTACGGCGAGGAGGTCTCCGACGAGGCGGAGGCGCGTGAGTTCAGTGAGCTGATGGAGGAGATTTCGTGCCACGGAGGAGCGTCGAACTGGGTGGATTTCATGCCGATTCTGAGATGGATTGGGTGCGACGGCGGATATGAAAGGAATTTGGCGAAATTGATGAAGAGGACGGATAAATTCATGGAGGCGCTGGTTGAAGAAGCGCGGCACAACAAGAAGAATTCAGCGATTGAGAGGAAGAATTGTTGTTCTCTGCTGGATCGTCTTCTGGAATTGCAGGAGTCGGAACCACAGTATTATACCGATCAGATTGTTAAAGGCCTCGTTCTGGTTAGCAATTTTATTGATTTCATTTAATTGCCTTCAATTAACTCCTTAATTGAATTTTTCTGGTAAGGATTTAGTTGGAAAAATATTGCTAAAATTACATGGATTTTGGAAAATACTGGGCTTTTTTTTAAGATTGGATGCTTGAGTTGTTTCACACCCAAGAAAGAGAAATAAAAAACAAATGTTTTTTTTTATTGTAAACTTTTCAATCGTGGTGTTATATCCATATATTTTTATATGTTTAAATTGATAAATTAAGACTAACCAAACGTGTCTATGGACTAGAAATACTAAGATTGTTTAGTGGAGGGATTAAATTGTTACCATTTCTAAATTTTTTTTAGCTAAGAATGCAATCAATTTAAAATTACAAGACTAAAGGGTTATTTAAGTGAAAATATAGATACTAAAAAGTATTTTTTTATTGTAATAAATAAAAATTACTTTTAAATATTCAATTCTTTAATTTTATCTTATCATTGAATTTAAAAAAAGAAAAAAAGTGTCTAAGTAATTTGGATCTTAACATGTCTTTTCAAAACGGTCTTTTAAGCTCACCAATCTTTACCCGTCTTGTTTAAACTTGTTGGTTTTTTCCTTCTTTTTTTGTTTTTTTTTTAAATTTGTTGAATTCATCAATATACATCTCAAAATCAAATCAATTGGTAAAAATGGAAGTAGCACATAGATCTTATCAACTCTGTTAGGTCATTTGATCTTTTGACGTGGGATACTTAATACTGACCGAATCCGAATTGAATAACCTAAACAGTAAACTTCATGAACTACGAATAACAAGAAGAAAAATGGAAGAATTTAATTAGTTCACACTCACTTTCTTGGAGTCAAATTTTGTTGTGTTCATGGTAACCATGAAAGTGAAATAATTTTTGATCGAGTGTGAGTATCTCTTCTCATTGAATGCAGTAGAATATGCAGGTTTTGCTGCGTGCAGGAACAGACTCGACGTCCGTGACCATGAACTGGGCAATGACCCAATTGCTCAACAATCCAGAAGTGTTGACGAAAGCCAAAGCTGAGTTGGACACAAAGGTTGGTCAAGATCGACTCGTCGACGAATCCGATCTCGCCAACCTCAAATACCTCCAAGCCATTGTTTTCGAGACCCTCCGCTTGCACCCGCCTGCTCCGATGCTCATCGCACATTACTCCTCCTCCGACTGCACAGTCGCTGGCTACCACATTCCACATGACACAATATTGTTGGTCAATGCGTGGGCGATTCATCGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTTCGGCCGGAGAGGTTTCTGGGATCAGCAGCAAATGAGTTGCTGTCCAACAAACTTATTGCATTTGGGCTCGGAAGGAGGGCCTGTCCTGGAGCAACTATGGGCGAACGGTTTGTGGGACTCACATTGGCGCTGATGATTCAATGTTATGAGTGGAAGAAAAGTGGAGAAGAAAATATTGACATGGGAGAGGGCGGCGGCATTACCATTCTCAAAGCTAAGCCTTTGGAAGTTATGTGTAAAGCTCGCCCTGTTATGGATAAAGTTCATGTTAAAGCGTAA

mRNA sequence

ATGGATTCTCTCTCTGTTTTCGCTCTGCTCCTCTTCGCACTCGCCTCAATCCTTTTTCGGCAGCAGATCAGAGCCCCGCGGCGGAATCTACCGCCGAGCCCGCCGTCTGTTCCGATCATCGGCCACCTCCGCCTCCTCCGCCGCCCCGTCCACCGCAACTTCCAGAAGATCGCCGCCGAATACGGCCCCATTTTCTCTCTCAGATTCGGATCTCGCCTCGCCGTCGTAGTCTCTTCACTCGACACAGCCGAAGAATGCCTAACGAAGAACGACGTCGTCTTCGCCAACCGGCCGCGCCTCATCATCGGCAAGCACCTCGGCTACAACCACACCACCATGGCCGCCGCGCCGTACGGCGCCCACTGGCGGAACCTCCGGCGCCTCACCGCCAGCGAGCTCTTCTCCACGGCCCGCCTCGGCTCCTCTCTGAGCATCCGGAAGGAGGAAATCCGGCGGGTGCTGCTGAAGCTCCGGGCCGGGTCGGGGTCCGGGTCGGGCGGGGACTTCGCGAAGGCGGAGTTGAAATCGATGTTCTCGGAGGTGACGTTCAACGTGGTGATGAGGATGGTGGCCGGAAAACGGTACTACGGCGAGGAGGTCTCCGACGAGGCGGAGGCGCGTGAGTTCAGTGAGCTGATGGAGGAGATTTCGTGCCACGGAGGAGCGTCGAACTGGGTGGATTTCATGCCGATTCTGAGATGGATTGGGTGCGACGGCGGATATGAAAGGAATTTGGCGAAATTGATGAAGAGGACGGATAAATTCATGGAGGCGCTGGTTGAAGAAGCGCGGCACAACAAGAAGAATTCAGCGATTGAGAGGAAGAATTGTTGTTCTCTGCTGGATCGTCTTCTGGAATTGCAGGAGTCGGAACCACAGTATTATACCGATCAGATTGTTAAAGGCCTCGTTCTGGTTTTGCTGCGTGCAGGAACAGACTCGACGTCCGTGACCATGAACTGGGCAATGACCCAATTGCTCAACAATCCAGAAGTGTTGACGAAAGCCAAAGCTGAGTTGGACACAAAGGTTGGTCAAGATCGACTCGTCGACGAATCCGATCTCGCCAACCTCAAATACCTCCAAGCCATTGTTTTCGAGACCCTCCGCTTGCACCCGCCTGCTCCGATGCTCATCGCACATTACTCCTCCTCCGACTGCACAGTCGCTGGCTACCACATTCCACATGACACAATATTGTTGGTCAATGCGTGGGCGATTCATCGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTTCGGCCGGAGAGGTTTCTGGGATCAGCAGCAAATGAGTTGCTGTCCAACAAACTTATTGCATTTGGGCTCGGAAGGAGGGCCTGTCCTGGAGCAACTATGGGCGAACGGTTTGTGGGACTCACATTGGCGCTGATGATTCAATGTTATGAGTGGAAGAAAAGTGGAGAAGAAAATATTGACATGGGAGAGGGCGGCGGCATTACCATTCTCAAAGCTAAGCCTTTGGAAGTTATGTGTAAAGCTCGCCCTGTTATGGATAAAGTTCATGTTAAAGCGTAA

Coding sequence (CDS)

ATGGATTCTCTCTCTGTTTTCGCTCTGCTCCTCTTCGCACTCGCCTCAATCCTTTTTCGGCAGCAGATCAGAGCCCCGCGGCGGAATCTACCGCCGAGCCCGCCGTCTGTTCCGATCATCGGCCACCTCCGCCTCCTCCGCCGCCCCGTCCACCGCAACTTCCAGAAGATCGCCGCCGAATACGGCCCCATTTTCTCTCTCAGATTCGGATCTCGCCTCGCCGTCGTAGTCTCTTCACTCGACACAGCCGAAGAATGCCTAACGAAGAACGACGTCGTCTTCGCCAACCGGCCGCGCCTCATCATCGGCAAGCACCTCGGCTACAACCACACCACCATGGCCGCCGCGCCGTACGGCGCCCACTGGCGGAACCTCCGGCGCCTCACCGCCAGCGAGCTCTTCTCCACGGCCCGCCTCGGCTCCTCTCTGAGCATCCGGAAGGAGGAAATCCGGCGGGTGCTGCTGAAGCTCCGGGCCGGGTCGGGGTCCGGGTCGGGCGGGGACTTCGCGAAGGCGGAGTTGAAATCGATGTTCTCGGAGGTGACGTTCAACGTGGTGATGAGGATGGTGGCCGGAAAACGGTACTACGGCGAGGAGGTCTCCGACGAGGCGGAGGCGCGTGAGTTCAGTGAGCTGATGGAGGAGATTTCGTGCCACGGAGGAGCGTCGAACTGGGTGGATTTCATGCCGATTCTGAGATGGATTGGGTGCGACGGCGGATATGAAAGGAATTTGGCGAAATTGATGAAGAGGACGGATAAATTCATGGAGGCGCTGGTTGAAGAAGCGCGGCACAACAAGAAGAATTCAGCGATTGAGAGGAAGAATTGTTGTTCTCTGCTGGATCGTCTTCTGGAATTGCAGGAGTCGGAACCACAGTATTATACCGATCAGATTGTTAAAGGCCTCGTTCTGGTTTTGCTGCGTGCAGGAACAGACTCGACGTCCGTGACCATGAACTGGGCAATGACCCAATTGCTCAACAATCCAGAAGTGTTGACGAAAGCCAAAGCTGAGTTGGACACAAAGGTTGGTCAAGATCGACTCGTCGACGAATCCGATCTCGCCAACCTCAAATACCTCCAAGCCATTGTTTTCGAGACCCTCCGCTTGCACCCGCCTGCTCCGATGCTCATCGCACATTACTCCTCCTCCGACTGCACAGTCGCTGGCTACCACATTCCACATGACACAATATTGTTGGTCAATGCGTGGGCGATTCATCGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTTCGGCCGGAGAGGTTTCTGGGATCAGCAGCAAATGAGTTGCTGTCCAACAAACTTATTGCATTTGGGCTCGGAAGGAGGGCCTGTCCTGGAGCAACTATGGGCGAACGGTTTGTGGGACTCACATTGGCGCTGATGATTCAATGTTATGAGTGGAAGAAAAGTGGAGAAGAAAATATTGACATGGGAGAGGGCGGCGGCATTACCATTCTCAAAGCTAAGCCTTTGGAAGTTATGTGTAAAGCTCGCCCTGTTATGGATAAAGTTCATGTTAAAGCGTAA

Protein sequence

MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAEYGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGAHWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSEVTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGGYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIVKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA

Homology

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BLAST of Moc02g12310 vs. NCBI nr
Match: XP_022149457.1 (cytochrome P450 81E8-like isoform X2 [Momordica charantia])

HSP 1 Score: 1023.1 bits (2644), Expect = 8.4e-295
Identity = 512/512 (100.00%), Postives = 512/512 (100.00%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY
Sbjct: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID
Sbjct: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 513
           MGEGGGITILKAKPLEVMCKARPVMDKVHVKA
Sbjct: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 512

[Close]

BLAST of Moc02g12310 vs. NCBI nr
Match: XP_022149456.1 (cytochrome P450 81E8-like isoform X1 [Momordica charantia])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.5e-293
Identity = 512/515 (99.42%), Postives = 512/515 (99.42%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVL---VLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360
           KGLVL   VLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN
Sbjct: 301 KGLVLNMQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360

Query: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420
           LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP
Sbjct: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420

Query: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480
           TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE
Sbjct: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480

Query: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA 513
           NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA
Sbjct: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA 515

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BLAST of Moc02g12310 vs. NCBI nr
Match: AEM42992.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 763.8 bits (1971), Expect = 9.2e-217
Identity = 388/512 (75.78%), Postives = 435/512 (84.96%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MD  S F LLL    ++L   QIR  RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1   MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG 
Sbjct: 61  YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL     SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           + FN +MR+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS+WVDFMPIL+WIG  GG
Sbjct: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           +E++L +L KRTDKFM+AL+EE R NKK   +ERKN  SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KGLVLVLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL NL Y
Sbjct: 301 KGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAIV ETLRLHP APML++HYSS+DCTVAGY IP  T LLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLG AANEL S KLIAFGLGRR+CPG TM  RFVGLTL L+IQCY+WKK G+E +D
Sbjct: 421 RPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVD 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 513
           MGEGGGITI KAKPLE MCKARP M K+ + A
Sbjct: 481 MGEGGGITIHKAKPLEAMCKARPAMYKLLLNA 498

[Close]

BLAST of Moc02g12310 vs. NCBI nr
Match: XP_022985607.1 (cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 725.7 bits (1872), Expect = 2.8e-205
Identity = 363/508 (71.46%), Postives = 425/508 (83.66%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MD LSVF LLL      LF QQ    RRNLPPSPPSVPIIGHL LL+RP+H+NF  IAA+
Sbjct: 1   MDFLSVFLLLLLV---FLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAK 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIF+LRFGSRLA++VSSL  AEEC TKNDVVFANRPRL+IGK+LGYN TT++  PYG 
Sbjct: 61  YGPIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGD 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
            WRNLRRLT+ E+FSTARL ++LSIRK+EIRR+L+KL + S       FAK ELKSMFSE
Sbjct: 121 LWRNLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES-------FAKVELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           ++FNVVMR+V GKRY+GEEVS+E EAREF ELM+EIS HGGASNW++FMPIL+WIG  G 
Sbjct: 181 LSFNVVMRVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGF-GE 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YE++LAKL KR DKFM+ LV E R N+    IE+++  +LLDRLLELQ SEP+YY+D+I+
Sbjct: 241 YEKSLAKLTKRADKFMQKLV-EYRRNQTGFEIEKQS--TLLDRLLELQASEPEYYSDEII 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KG+VLVLLRAG DSTSVTM WAMTQLLNNPEV++KAKAE+DTK+G+DRLVDESDLANL Y
Sbjct: 301 KGVVLVLLRAGVDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAI++ET RLHP APML+ HYSS DCTVAGY IP  T+L+VNAWAIHRDPKLWDDP SF
Sbjct: 361 LQAIIYETFRLHPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLG A NELLSNKL+ FG+GRRACPG TM +R +GL+L L+IQCYEWK  G + +D
Sbjct: 421 RPERFLG-AGNELLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVD 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKV 509
           M EGGGITI K +PLE MC+A P+MDKV
Sbjct: 481 MTEGGGITIQKVQPLETMCRALPIMDKV 493

[Close]

BLAST of Moc02g12310 vs. NCBI nr
Match: XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 719.5 bits (1856), Expect = 2.0e-203
Identity = 358/508 (70.47%), Postives = 421/508 (82.87%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MD LSV  LLL      LF QQ    RRNLPPSPPSVPIIGHL LL+RP+HRNF  IAA+
Sbjct: 1   MDFLSVLLLLLLV---FLFLQQFITCRRNLPPSPPSVPIIGHLHLLQRPIHRNFHHIAAK 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIF+LRFGSRLA++VSSL  AEEC TKNDVVFANRPRL+IGK+LGYN TT++  PYG 
Sbjct: 61  YGPIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGD 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRLT+ E+FSTARL ++LSIRK+EIRR+L+KL + S       FA+ ELKSMFSE
Sbjct: 121 HWRNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES-------FARVELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           ++FNVVMR+V GKRY+GEEVS+E EAREF ELM+E S HGGASNW+DFMPIL+WIG  G 
Sbjct: 181 LSFNVVMRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWIDFMPILKWIGF-GE 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YE++LAKL KR DKFM+ LVE+ R N+    IE+++  +LLDRLLELQ SEP+YY+D+I+
Sbjct: 241 YEKSLAKLTKRADKFMQKLVED-RRNQTGLQIEKQS--TLLDRLLELQASEPEYYSDEII 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KG++LVLLRAG DSTSVTM WAMTQLLNNPEV++KAKAE+DTK+G+DRLVDE+DL NL Y
Sbjct: 301 KGVILVLLRAGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAI+ ET RLHP APML+ HYSS DCTVAGY IP  T+LLVNAWAIHRDPKLWDDP  F
Sbjct: 361 LQAIISETFRLHPAAPMLLTHYSSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLG   NELLSNKLI FG+GRRACPG TM +R +GL+L L+IQCY+WK  G + +D
Sbjct: 421 RPERFLGE-GNELLSNKLIPFGMGRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVD 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKV 509
           M EGGGITI K +PLE +C+A P+MDKV
Sbjct: 481 MTEGGGITIQKVQPLETLCRALPIMDKV 493

[Close]

BLAST of Moc02g12310 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.5e-150
Identity = 265/502 (52.79%), Postives = 361/502 (71.91%), Query Frame = 0


Query: 8   ALLLFALASILFRQQIRAPRRNLPPSPP-SVPIIGHLRLLRRPVHRNFQKIAAEYGPIFS 67
           A++L +++  LF   +   RRNLPPSP  ++P+IGHL L+ + +HR+   ++ +YG +FS
Sbjct: 12  AVVLLSISLKLF--PVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFS 71

Query: 68  LRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGAHWRNLR 127
           L+ G+RL +VVSS   AEEC TKND+VFANRP  I+GK++GYN+TTM  +PYG HWRNLR
Sbjct: 72  LQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLR 131

Query: 128 RLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSEVTFNVV 187
           RL A E+FS   L   LSIR++E++++LL L   SG     DF K E+KS  SE++FNV 
Sbjct: 132 RLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQ----DFGKVEMKSKLSELSFNVT 191

Query: 188 MRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGGYERNLA 247
           MRMVAGKRY+G++V D  EA+ F  L+ E+  H GASN  DF+P LRWI     YE+ ++
Sbjct: 192 MRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFK-NYEKKVS 251

Query: 248 KLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIVKGLVLV 307
           K+ +  D F++ L+ E+R NK N         +++D LL LQES+P+YYTDQI+KG+++V
Sbjct: 252 KISQEMDAFLQRLIHESRINKNN--------VTMIDHLLSLQESQPEYYTDQIIKGIIMV 311

Query: 308 LLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAIVF 367
           LL AGTD+++VT+ WAM+ LLN+PE L KA+ E++T+VG +RL++E DL  L YL  I+ 
Sbjct: 312 LLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIIS 371

Query: 368 ETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPERFL 427
           ET RL P APML+ H SS DC V GY +P  TILLVNAWAIHRDP+ WD+PT F+PER  
Sbjct: 372 ETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHG 431

Query: 428 GSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEGGG 487
           G    EL  +KL+ FG+GRR+CPG+ + +R VGLTL  +IQC+EWK+ GE  IDM EG G
Sbjct: 432 GV---ELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSG 491

Query: 488 ITILKAKPLEVMCKARPVMDKV 509
           +T+ KA+PLE +CK R ++ KV
Sbjct: 492 LTMPKAQPLEALCKPRNILHKV 494

[Close]

BLAST of Moc02g12310 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 266/501 (53.09%), Postives = 352/501 (70.26%), Query Frame = 0


Query: 4   LSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAEYGP 63
           LS+   L F + ++          +NLPP P  +PIIG+L  L++P+H  F  ++ +YG 
Sbjct: 6   LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQ 65

Query: 64  IFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGAHWR 123
           IFSL FGSRL VVVSSL  A+EC TKND+V ANRP  + GK++GYN+TT+A +PYG HWR
Sbjct: 66  IFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWR 125

Query: 124 NLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSEVTF 183
           NLRR+ + E+ S+ RL S L IR++EI R++ KL   S +G    F + EL+ MFSE+TF
Sbjct: 126 NLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNG----FTEVELRPMFSEMTF 185

Query: 184 NVVMRMVAGKRYYGE--EVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGGY 243
           N +MRMV+GKRYYG   +VSD  EAR F  +++E+   GGA+N  DF+  LRW   D G 
Sbjct: 186 NTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GL 245

Query: 244 ERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIVK 303
           E+ L K+ KRTD F++ L++E R  K+NS        +++D LL  Q+S+P+YYTDQI+K
Sbjct: 246 EKRLKKISKRTDAFLQGLIDEHRFGKRNSN-------TMIDHLLTQQQSQPEYYTDQIIK 305

Query: 304 GLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYL 363
           GL++V+L AGTD++SVT+ WAM+ LLN+PE++ KAK ELDT +G DR VDE D++ L YL
Sbjct: 306 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 365

Query: 364 QAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFR 423
           Q+IV+ETLRLH  AP+L+ H SS D ++ GY+IP +TIL+VNAW IHRDP LW DPT F+
Sbjct: 366 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 425

Query: 424 PERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDM 483
           PERF      E   NKL++FGLGRRACPG  + +R  GLTL L+IQC+EWK+ GEE IDM
Sbjct: 426 PERF----EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDM 485

Query: 484 GEGGGITILKAKPLEVMCKAR 503
            E  GIT  K   L  MCK R
Sbjct: 486 VEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Moc02g12310 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-143
Identity = 258/508 (50.79%), Postives = 357/508 (70.28%), Query Frame = 0


Query: 3   SLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAEYG 62
           S SVF L LF + +I+ R +     +NLPP PPS+PIIG+L  L+RP+HR F+ ++ +YG
Sbjct: 8   SYSVFYLALFFIFNIVIRAR---KFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYG 67

Query: 63  PIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGAHW 122
            +FSL FGSRL VVVSS    ++C TKNDVV ANRPR + GK++ YN+TT+ +  YG HW
Sbjct: 68  HVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 127

Query: 123 RNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSEVT 182
           RNLRR+TA ++ S  R+ S   IR++E +R++ +L       S  +FA+ EL S   ++T
Sbjct: 128 RNLRRITALDVLSNHRINSFSGIRRDETQRLITRL----ADDSSTNFAEMELSSRLYDMT 187

Query: 183 FNVVMRMVAGKRYYGE--EVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 242
           FN +MRM++GKRYYGE  + SD  EA +F +++ E+    GA+N  DFMP+LR++  +  
Sbjct: 188 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE-N 247

Query: 243 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 302
            E+ L  +  +TD F+  L+EE R  K     ER N  +++D LL LQ+S+P+YYTDQI+
Sbjct: 248 LEKRLKDISGKTDAFLRGLIEEHRTKK-----ERAN--TMIDHLLNLQDSQPEYYTDQII 307

Query: 303 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 362
           KGL L +L AGTDS++VT+ W+M+ LLN+PEVL K K ELDT VGQDRLVDESDL  L Y
Sbjct: 308 KGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTY 367

Query: 363 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 422
           L+ ++ ETLRL+ PAP+L+ H +S +C + GY +P DTI+L+NAWAIHRDP+LW + T+F
Sbjct: 368 LKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTF 427

Query: 423 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 482
           +PERF      EL   KLIAFG+GRRACPG  +  R + +TLAL+IQC++WK    + ID
Sbjct: 428 KPERF--EKKGEL--EKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKID 487

Query: 483 MGEGGGITILKAKPLEVMCKARPVMDKV 509
           + E  G T+ K  PL+ MCK+RPV++KV
Sbjct: 488 LAERDGFTLTKLVPLKAMCKSRPVINKV 496

[Close]

BLAST of Moc02g12310 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.0e-141
Identity = 253/510 (49.61%), Postives = 354/510 (69.41%), Query Frame = 0


Query: 8   ALLLFALASILFRQQIR-----APRR--NLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 67
           AL  ++L S+ F   I+       RR  NLPP PP++PIIG+L  L+ P+HR F  ++  
Sbjct: 1   ALFYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQT 60

Query: 68  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 127
           YG IFSL FGSRL VVVSS   A EC TKND++ ANRPR + GK++ YN+TT+ +A YG 
Sbjct: 61  YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 120

Query: 128 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 187
           HWRNLRR+T  ++ S  RL S L +R++E  R++ KL     S  G  F K EL+   +E
Sbjct: 121 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVS-EGFGFTKVELRPRLTE 180

Query: 188 VTFNVVMRMVAGKRYYGE--EVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCD 247
           +TFN +MRM++GKRYYG+  +VSD  EA++F E++ E+    GA+N  DF+P+LR +  D
Sbjct: 181 MTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD 240

Query: 248 GGYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQ 307
              E+   ++ KR++ F+E L+EE R    +S     +  +++D LL+L ES+P+YY+D 
Sbjct: 241 -NLEKRCKRIAKRSNAFLEGLIEEHRRGNIHS-----DGGTMIDHLLKLSESQPEYYSDH 300

Query: 308 IVKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANL 367
           ++KGL+  +L AGTD+++VT+ W M++LLN+PEVL KAK ELDT++G+++LVDE DL+ L
Sbjct: 301 LIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKL 360

Query: 368 KYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPT 427
            YLQ I+ ETLRLHPPAP+L+ HYSS DCT+  +++P DTI+L N W IHRDPK W+D  
Sbjct: 361 PYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDAL 420

Query: 428 SFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEEN 487
           SF+PERF      E   NK++AFGLGRRACPG ++ +R VG T+ L+IQC+EW++  EE 
Sbjct: 421 SFKPERF----EKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEK 480

Query: 488 IDMGEGGGITILKAKPLEVMCKARPVMDKV 509
           +DM EG GIT+    PL  MCKA P+ + V
Sbjct: 481 LDMMEGKGITMPMKIPLRAMCKALPIANDV 499

[Close]

BLAST of Moc02g12310 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.0e-138
Identity = 249/511 (48.73%), Postives = 352/511 (68.88%), Query Frame = 0


Query: 5   SVFALLLFALASILFRQQIRAPRRNLPPSPPS-VPIIGHLRLLRRPVHRNFQKIAA---- 64
           S+F+ L+F + S  F   ++  ++NLPPSPP  +PIIGHLRLL+ P+HR  +  +     
Sbjct: 12  SIFS-LIFLIISFKF---LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 71

Query: 65  -EYGPIFSLRFGSRLAVVVSSLD-TAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAP 124
            + G + SLR GSRL  VVSS    AEEC  KNDVV ANRP++IIGKH+GYN+T M AAP
Sbjct: 72  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 131

Query: 125 YGAHWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSM 184
           YG HWRNLRRL   E+FST RL   L +R +E+RR++ +L   +G+         ELK M
Sbjct: 132 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK----TVVELKPM 191

Query: 185 FSEVTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGC 244
             ++TFN +MRM+ GKRYYGEE +DE EA+   +L+ ++  +  + N VD++PILR    
Sbjct: 192 LMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF-- 251

Query: 245 DGGYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTD 304
              YE  + KL + TDKF++ L+++ R  ++          +++D LL LQ+S+ +YYTD
Sbjct: 252 -SSYENRVKKLGEETDKFLQGLIDDKRGQQETGT-------TMIDHLLVLQKSDIEYYTD 311

Query: 305 QIVKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 364
           QI+KG++L+++ AGT++++VT+ WA++ LLN+P+V++KA+ E+D +VG DRL++E+DL+ 
Sbjct: 312 QIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSE 371

Query: 365 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 424
           L YL+ IV ETLRLHP  P+L+ H +S DC +  Y +P  T LLVNAWAIHRDP  WDDP
Sbjct: 372 LPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDP 431

Query: 425 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 484
            SF+PERF      E  + KL+AFGLGRRACPG+ + +R VGL L  +IQC+EW++ G  
Sbjct: 432 DSFKPERF----EKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNV 491

Query: 485 NIDMGEGGGITILKAKPLEVMCKARPVMDKV 509
            +DM EG G T+ KA PL+ +CKARP + K+
Sbjct: 492 EVDMKEGVGNTVPKAIPLKAICKARPFLHKI 500

[Close]

BLAST of Moc02g12310 vs. ExPASy TrEMBL
Match: A0A6J1D806 (cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 4.1e-295
Identity = 512/512 (100.00%), Postives = 512/512 (100.00%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY
Sbjct: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID
Sbjct: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 513
           MGEGGGITILKAKPLEVMCKARPVMDKVHVKA
Sbjct: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 512

[Close]

BLAST of Moc02g12310 vs. ExPASy TrEMBL
Match: A0A6J1D8F1 (cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017882 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.7e-293
Identity = 512/515 (99.42%), Postives = 512/515 (99.42%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE
Sbjct: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA
Sbjct: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE
Sbjct: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG
Sbjct: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV
Sbjct: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300

Query: 301 KGLVL---VLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360
           KGLVL   VLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN
Sbjct: 301 KGLVLNMQVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360

Query: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420
           LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP
Sbjct: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420

Query: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480
           TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE
Sbjct: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480

Query: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA 513
           NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA
Sbjct: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKVHVKA 515

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BLAST of Moc02g12310 vs. ExPASy TrEMBL
Match: K7NC01 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.4e-217
Identity = 388/512 (75.78%), Postives = 435/512 (84.96%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MD  S F LLL    ++L   QIR  RRNLPPSPPS+PIIGHL LL+RP+HRNF KIAAE
Sbjct: 1   MDFFSAFLLLLL---TVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAE 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIFSLRFGSRLAV+VSSLD AEEC TKND++FANRPRL+I KHLGYN TTMA +PYG 
Sbjct: 61  YGPIFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGD 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRL A E+FSTARL SSLSIRK+EI+R+LLKL     SGS G+F K ELK+MFSE
Sbjct: 121 HWRNLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLH----SGSSGEFTKVELKTMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           + FN +MR+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS+WVDFMPIL+WIG  GG
Sbjct: 181 LAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIG-GGG 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           +E++L +L KRTDKFM+AL+EE R NKK   +ERKN  SLLDRLLELQ SEP+YYTDQI+
Sbjct: 241 FEKSLVRLTKRTDKFMQALIEE-RRNKK--VLERKN--SLLDRLLELQASEPEYYTDQII 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KGLVLVLLRAGTD+++VT+NWAM QLLNNPE+L KAKAELDTK+GQDR VDE DL NL Y
Sbjct: 301 KGLVLVLLRAGTDTSAVTLNWAMAQLLNNPELLAKAKAELDTKIGQDRPVDEPDLPNLSY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAIV ETLRLHP APML++HYSS+DCTVAGY IP  T LLVNAWAIHRDPKLWDDPTSF
Sbjct: 361 LQAIVSETLRLHPAAPMLLSHYSSADCTVAGYDIPRGTTLLVNAWAIHRDPKLWDDPTSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLG AANEL S KLIAFGLGRR+CPG TM  RFVGLTL L+IQCY+WKK G+E +D
Sbjct: 421 RPERFLG-AANELQSKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGDEKVD 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKVHVKA 513
           MGEGGGITI KAKPLE MCKARP M K+ + A
Sbjct: 481 MGEGGGITIHKAKPLEAMCKARPAMYKLLLNA 498

[Close]

BLAST of Moc02g12310 vs. ExPASy TrEMBL
Match: A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.3e-205
Identity = 363/508 (71.46%), Postives = 425/508 (83.66%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MD LSVF LLL      LF QQ    RRNLPPSPPSVPIIGHL LL+RP+H+NF  IAA+
Sbjct: 1   MDFLSVFLLLLLV---FLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAK 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIF+LRFGSRLA++VSSL  AEEC TKNDVVFANRPRL+IGK+LGYN TT++  PYG 
Sbjct: 61  YGPIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGD 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
            WRNLRRLT+ E+FSTARL ++LSIRK+EIRR+L+KL + S       FAK ELKSMFSE
Sbjct: 121 LWRNLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSES-------FAKVELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           ++FNVVMR+V GKRY+GEEVS+E EAREF ELM+EIS HGGASNW++FMPIL+WIG  G 
Sbjct: 181 LSFNVVMRVVTGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGF-GE 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YE++LAKL KR DKFM+ LV E R N+    IE+++  +LLDRLLELQ SEP+YY+D+I+
Sbjct: 241 YEKSLAKLTKRADKFMQKLV-EYRRNQTGFEIEKQS--TLLDRLLELQASEPEYYSDEII 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KG+VLVLLRAG DSTSVTM WAMTQLLNNPEV++KAKAE+DTK+G+DRLVDESDLANL Y
Sbjct: 301 KGVVLVLLRAGVDSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAI++ET RLHP APML+ HYSS DCTVAGY IP  T+L+VNAWAIHRDPKLWDDP SF
Sbjct: 361 LQAIIYETFRLHPAAPMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLG A NELLSNKL+ FG+GRRACPG TM +R +GL+L L+IQCYEWK  G + +D
Sbjct: 421 RPERFLG-AGNELLSNKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVD 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKV 509
           M EGGGITI K +PLE MC+A P+MDKV
Sbjct: 481 MTEGGGITIQKVQPLETMCRALPIMDKV 493

[Close]

BLAST of Moc02g12310 vs. ExPASy TrEMBL
Match: A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.8e-203
Identity = 359/508 (70.67%), Postives = 420/508 (82.68%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPPSVPIIGHLRLLRRPVHRNFQKIAAE 60
           MD L V  LLL      LF QQ    RRNLPPSPPS PIIGHL LL+RP+HRNF  IAA+
Sbjct: 1   MDFLFVLLLLLLV---FLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAK 60

Query: 61  YGPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGA 120
           YGPIF+LRFGSRLA++VSSL  AEEC TKNDVVFANRPRL+IGK+LGYN TT++  P+G 
Sbjct: 61  YGPIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGD 120

Query: 121 HWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSE 180
           HWRNLRRLT+ E+FSTARL ++LSIRK+EIRR+L+KL + S       FAK ELKSMFSE
Sbjct: 121 HWRNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES-------FAKVELKSMFSE 180

Query: 181 VTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGG 240
           ++FNVVMR+V GKRY+GEEVS+E EAREF ELM+E S HGGASNWVDFMPIL+WIG  G 
Sbjct: 181 LSFNVVMRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGF-GE 240

Query: 241 YERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIV 300
           YE++LAKL KR DKFM+ LVE+ R N+    IE+++  +LLDRLLELQ SEP+YY+D+I+
Sbjct: 241 YEKSLAKLTKRADKFMQKLVED-RRNQTGLQIEKQS--TLLDRLLELQASEPEYYSDEII 300

Query: 301 KGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKY 360
           KG++LVLLRAG DSTSVTM WAMTQLLNNPEV++KAKAE+DTK+G+DRL+DE+DLANL Y
Sbjct: 301 KGVILVLLRAGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHY 360

Query: 361 LQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSF 420
           LQAI+ ET RLHP APML+ HYSS+DCTVAGY +P  T+LLVNAWAIHRDPKLWDDP SF
Sbjct: 361 LQAIISETFRLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSF 420

Query: 421 RPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENID 480
           RPERFLG   NELL NKLI FG+GRRACPG TM  R +GL+L L+IQCYEWK  G E +D
Sbjct: 421 RPERFLGE-GNELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVD 480

Query: 481 MGEGGGITILKAKPLEVMCKARPVMDKV 509
           M EGGGITI K +PLE MC+A P+MDKV
Sbjct: 481 MTEGGGITIQKVQPLETMCRALPIMDKV 493

[Close]

BLAST of Moc02g12310 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 518.1 bits (1333), Expect = 8.2e-147
Identity = 264/511 (51.66%), Postives = 359/511 (70.25%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPP-SVPIIGHLRLLRRPVHRNFQKIAA 60
           M++ ++   +LF + S+++       + NLPPSP  S+P+IGHLRLL+ P+HR F  ++ 
Sbjct: 1   METKTLIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQ 60

Query: 61  EY--GPIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAP 120
                PIFSLR G+RL  V SS   AEEC TKNDVV ANRP  I+ KH+ Y++TTM AA 
Sbjct: 61  SLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAAS 120

Query: 121 YGAHWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSM 180
           YG HWRNLRR+ + E+FS  RL S LSIRK+EIRR++ +L          +F K ++KSM
Sbjct: 121 YGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQ----EFVKVDMKSM 180

Query: 181 FSEVTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGC 240
            S++TFN ++RMVAGKRYYG+ V D+ EA+   +L+ ++    GA N VD++P+LR +  
Sbjct: 181 LSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV-- 240

Query: 241 DGGYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTD 300
              YE  + KL  R D+F++ LV+E R  K     E+ N  +++D LL LQES+P Y+TD
Sbjct: 241 -SDYETRVKKLAGRLDEFLQGLVDEKREAK-----EKGN--TMIDHLLTLQESQPDYFTD 300

Query: 301 QIVKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360
           +I+KG +L L+ AGTD+++VT+ WA++ +LN+P+VL KA+ E+D K+G DRL+DESD++N
Sbjct: 301 RIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISN 360

Query: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420
           L YLQ IV ETLRL+P APML+ H +S DC VAGY +P  TILL N WAIHRDP+LWDDP
Sbjct: 361 LPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDP 420

Query: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480
            SF+PERF      E  + KL+ FGLGRRACPG+ +  R + LTL  +IQC EW+K GEE
Sbjct: 421 MSFKPERF----EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE 480

Query: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKV 509
            +DM EG G+T+ KAKPLE MC+ARP + K+
Sbjct: 481 -VDMSEGKGVTMPKAKPLEAMCRARPSVVKI 492

[Close]

BLAST of Moc02g12310 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-140
Identity = 257/507 (50.69%), Postives = 351/507 (69.23%), Query Frame = 0


Query: 6   VFALLLFALASILFRQQIRAPRRNLPPSPP-SVPIIGHLRLLRRPVHRNFQKIAAEYG-- 65
           +F  L  +L+      +I+  R NLPPSP  ++P+IGHLRLL+ P+HR F  ++   G  
Sbjct: 7   IFTFLFISLSLTFIIGRIKR-RPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDA 66

Query: 66  PIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGAHW 125
           PI SLR G+RL  VVSS   AEEC TKNDVV ANR   +  KH+ Y  TT+  A YG HW
Sbjct: 67  PIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHW 126

Query: 126 RNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSEVT 185
           RNLRR+ A E+FS  RL S  SIR++EI R++  L   S      +F K E+KSMFS +T
Sbjct: 127 RNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSL----EFTKVEMKSMFSNLT 186

Query: 186 FNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGGYE 245
           FN ++RM+AGK YYG+   D+ EA+   EL+ E     GA N  D++PIL WI    G E
Sbjct: 187 FNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSE 246

Query: 246 RNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIVKG 305
           + + K+  R D+F++ LV+E R  K+    +R+N  +++D LL LQE++P+YYTD I+KG
Sbjct: 247 KRIKKIASRLDEFLQGLVDERREGKE----KRQN--TMVDHLLCLQETQPEYYTDNIIKG 306

Query: 306 LVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQ 365
           ++L L+ AGTD+++VT+ W ++ LLN+P++L+KA+ E+D KVG +RLV+ESDL++L YLQ
Sbjct: 307 IMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQ 366

Query: 366 AIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRP 425
            IV E+LRL+P +P+L+ H +S DC V GYH+P  T+LL NAWAIHRDPK+WDDPTSF+P
Sbjct: 367 NIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKP 426

Query: 426 ERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMG 485
           ERF      E  + KL+ FGLGRRACPG+ + +R   LT+  +IQC+EW++ GEE +DM 
Sbjct: 427 ERF----EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMT 486

Query: 486 EGGGITIL-KAKPLEVMCKARPVMDKV 509
           EGGG  I+ KA PL  MCKARPV+ K+
Sbjct: 487 EGGGGVIMPKAIPLVAMCKARPVVGKI 495

[Close]

BLAST of Moc02g12310 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 495.0 bits (1273), Expect = 7.4e-140
Identity = 249/511 (48.73%), Postives = 352/511 (68.88%), Query Frame = 0


Query: 5   SVFALLLFALASILFRQQIRAPRRNLPPSPPS-VPIIGHLRLLRRPVHRNFQKIAA---- 64
           S+F+ L+F + S  F   ++  ++NLPPSPP  +PIIGHLRLL+ P+HR  +  +     
Sbjct: 12  SIFS-LIFLIISFKF---LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 71

Query: 65  -EYGPIFSLRFGSRLAVVVSSLD-TAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAP 124
            + G + SLR GSRL  VVSS    AEEC  KNDVV ANRP++IIGKH+GYN+T M AAP
Sbjct: 72  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 131

Query: 125 YGAHWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSM 184
           YG HWRNLRRL   E+FST RL   L +R +E+RR++ +L   +G+         ELK M
Sbjct: 132 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK----TVVELKPM 191

Query: 185 FSEVTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGC 244
             ++TFN +MRM+ GKRYYGEE +DE EA+   +L+ ++  +  + N VD++PILR    
Sbjct: 192 LMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF-- 251

Query: 245 DGGYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTD 304
              YE  + KL + TDKF++ L+++ R  ++          +++D LL LQ+S+ +YYTD
Sbjct: 252 -SSYENRVKKLGEETDKFLQGLIDDKRGQQETGT-------TMIDHLLVLQKSDIEYYTD 311

Query: 305 QIVKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 364
           QI+KG++L+++ AGT++++VT+ WA++ LLN+P+V++KA+ E+D +VG DRL++E+DL+ 
Sbjct: 312 QIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSE 371

Query: 365 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 424
           L YL+ IV ETLRLHP  P+L+ H +S DC +  Y +P  T LLVNAWAIHRDP  WDDP
Sbjct: 372 LPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDP 431

Query: 425 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 484
            SF+PERF      E  + KL+AFGLGRRACPG+ + +R VGL L  +IQC+EW++ G  
Sbjct: 432 DSFKPERF----EKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNV 491

Query: 485 NIDMGEGGGITILKAKPLEVMCKARPVMDKV 509
            +DM EG G T+ KA PL+ +CKARP + K+
Sbjct: 492 EVDMKEGVGNTVPKAIPLKAICKARPFLHKI 500

[Close]

BLAST of Moc02g12310 vs. TAIR 10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )

HSP 1 Score: 494.6 bits (1272), Expect = 9.7e-140
Identity = 246/505 (48.71%), Postives = 342/505 (67.72%), Query Frame = 0


Query: 8   ALLLFALASILFRQQIRAPRRNLPPSPP-SVPIIGHLRLLRRPVHRNFQKIAAEYG--PI 67
           A  LF    +LF +  R  + NLPPSP   +P+IGHL LL+ P+HR F   +   G  PI
Sbjct: 26  AFFLFISVKLLFGK--RLSKFNLPPSPARPLPLIGHLHLLKLPLHRTFLSFSQSLGGAPI 85

Query: 68  FSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAPYGAHWRN 127
           F LR G+RL VVVSS   AEEC TKND+VFANRP LI+GKH+ YN TTM +APYG HWRN
Sbjct: 86  FCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGDHWRN 145

Query: 128 LRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSMFSEVTFN 187
           LRR+   E+F++ +L   LS+RK+EIR++LL+L   S  G    FAK E++ +  E+T N
Sbjct: 146 LRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHG----FAKVEMRHLLFELTIN 205

Query: 188 VVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGCDGGYERN 247
            V RMVAGKR+YGE    +  A++   LM+EI    GA N  D++PI+RW      +E+ 
Sbjct: 206 NVFRMVAGKRFYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRWF---TNFEKR 265

Query: 248 LAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTDQIVKGLV 307
           +  L  R DKF+++LV+E R +K+          +++D LL LQES+P YYTD  +KG++
Sbjct: 266 VKNLAIRIDKFLQSLVDEKRADKEKGT-------TMIDHLLSLQESQPDYYTDVTLKGII 325

Query: 308 LVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLANLKYLQAI 367
           +V++ AG+++ + T+ WAM  +LN+PEVL KA+ E+DTK+G DRL+DE+D  NL YLQ I
Sbjct: 326 IVMIIAGSETIAWTLEWAMLNVLNHPEVLKKARTEIDTKIGFDRLMDEADTKNLPYLQWI 385

Query: 368 VFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDPTSFRPER 427
           V ETLRLHP AP  + H +S DC +AGY +P  ++LLVN W++HRDP +W+DP  F+PER
Sbjct: 386 VLETLRLHPAAPTNVPHSTSEDCMLAGYDVPRGSMLLVNIWSMHRDPSIWEDPEMFKPER 445

Query: 428 FLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEENIDMGEG 487
           F     NE L+ KL++FG+GRRACPG  +  R V LTL  M+QC+EW++ G+E +D  E 
Sbjct: 446 F----KNEKLNQKLLSFGMGRRACPGYGLAHRVVSLTLGSMVQCFEWQRIGQEYVDNSED 505

Query: 488 GGITILK-AKPLEVMCKARPVMDKV 509
             + +++   PL  MCKARP++ ++
Sbjct: 506 KTVVLMRPTTPLLAMCKARPIVHEI 510

[Close]

BLAST of Moc02g12310 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 493.4 bits (1269), Expect = 2.2e-139
Identity = 255/511 (49.90%), Postives = 348/511 (68.10%), Query Frame = 0


Query: 1   MDSLSVFALLLFALASILFRQQIRAPRRNLPPSPP-SVPIIGHLRLLRRPVHRNFQKIAA 60
           M++L +     F + S++F       + NLPPSP  ++P+IGHLRLL+ P+HR F  ++ 
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQ 60

Query: 61  EYG--PIFSLRFGSRLAVVVSSLDTAEECLTKNDVVFANRPRLIIGKHLGYNHTTMAAAP 120
             G  PI SLR G+RL  VVSS   AEEC TKNDV+ ANR   I  KH+ Y ++T+ +A 
Sbjct: 61  SLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSAS 120

Query: 121 YGAHWRNLRRLTASELFSTARLGSSLSIRKEEIRRVLLKLRAGSGSGSGGDFAKAELKSM 180
           Y  HWRNLRR+ A E+FS  RL S  SIR++EIRR++ +L   S  G    F K E+KSM
Sbjct: 121 YSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYG----FTKVEMKSM 180

Query: 181 FSEVTFNVVMRMVAGKRYYGEEVSDEAEAREFSELMEEISCHGGASNWVDFMPILRWIGC 240
           FS++TFN ++RM+AGK YYG+   D+ EA+    L+ E     G  N  D++PIL WI  
Sbjct: 181 FSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITY 240

Query: 241 DGGYERNLAKLMKRTDKFMEALVEEARHNKKNSAIERKNCCSLLDRLLELQESEPQYYTD 300
               E  + KL  R D+F++ LV+E R  K+    +++N  +++D LL LQE++P+YY D
Sbjct: 241 S---ETRIKKLAGRLDEFLQGLVDEKREGKE----KKEN--TMVDHLLCLQETQPEYYMD 300

Query: 301 QIVKGLVLVLLRAGTDSTSVTMNWAMTQLLNNPEVLTKAKAELDTKVGQDRLVDESDLAN 360
           +I+KG +L L+  GTD+T+VT+ WA++ LLNNPEVL KA+ E+D  +G DRL++ESD+ N
Sbjct: 301 RIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPN 360

Query: 361 LKYLQAIVFETLRLHPPAPMLIAHYSSSDCTVAGYHIPHDTILLVNAWAIHRDPKLWDDP 420
           L YLQ IV ETLRL+P APML+ H +S DC V GY +P  T+LL NAWAIHRDP LWDDP
Sbjct: 361 LPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDP 420

Query: 421 TSFRPERFLGSAANELLSNKLIAFGLGRRACPGATMGERFVGLTLALMIQCYEWKKSGEE 480
           TSF+PERF      E  + KL+ FGLGRRACPG+ + +R V L+L  +IQC+EW++ GEE
Sbjct: 421 TSFKPERF----EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEE 480

Query: 481 NIDMGEGGGITILKAKPLEVMCKARPVMDKV 509
            +DM EG G+T+ KA+PLE MC+AR  + K+
Sbjct: 481 EVDMTEGPGLTMPKARPLEAMCRARDFVGKI 494

The following BLAST results are available for this feature:

BLAST of Moc02g12310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149457.1	8.4e-295	100.00	cytochrome P450 81E8-like isoform X2 [Momordica charantia]	[more]
XP_022149456.1	3.5e-293	99.42	cytochrome P450 81E8-like isoform X1 [Momordica charantia]	[more]
AEM42992.1	9.2e-217	75.78	cytochrome P450 [Siraitia grosvenorii]	[more]
XP_022985607.1	2.8e-205	71.46	cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E...	[more]
XP_023512643.1	2.0e-203	70.47	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc02g12310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	4.5e-150	52.79	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	1.5e-148	53.09	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
P93147	3.1e-143	50.79	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ9	1.0e-141	49.61	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q9FG65	1.0e-138	48.73	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]

BLAST of Moc02g12310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D806	4.1e-295	100.00	cytochrome P450 81E8-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1D8F1	1.7e-293	99.42	cytochrome P450 81E8-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
K7NC01	4.4e-217	75.78	Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-1 PE=2 SV=1	[more]
A0A6J1J5B5	1.3e-205	71.46	cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1	[more]
A0A6J1FUQ9	2.8e-203	70.67	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=...	[more]

BLAST of Moc02g12310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	8.2e-147	51.66	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37340.1	1.5e-140	50.69	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT5G36220.1	7.4e-140	48.73	cytochrome p450 81d1	[more]
AT2G23220.1	9.7e-140	48.71	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT4G37360.1	2.2e-139	49.90	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 439..448 score: 51.99 coord: 310..327 score: 34.65 coord: 363..374 score: 42.78
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 31..490 e-value: 1.1E-94 score: 317.8
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 299..316 score: 32.76 coord: 82..103 score: 28.12 coord: 438..448 score: 49.59 coord: 448..471 score: 24.12 coord: 319..345 score: 33.69 coord: 181..199 score: 23.44 coord: 58..77 score: 39.13 coord: 362..380 score: 32.82 coord: 403..427 score: 42.58
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..511 e-value: 5.4E-123 score: 413.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 31..502
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 7..507
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 7..507
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 441..450

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g12310.1	Moc02g12310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen