Moc02g12230 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc02g12230 vs. NCBI nr
Match: XP_022149533.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Momordica charantia])

HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 810/810 (100.00%), Postives = 810/810 (100.00%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQ 60
           MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQ
Sbjct: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQ 60

Query: 61  SNHHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALF 120
           SNHHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALF
Sbjct: 61  SNHHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALF 120

Query: 121 VAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFAS 180
           VAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFAS
Sbjct: 121 VAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFAS 180

Query: 181 IFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITD 240
           IFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITD
Sbjct: 181 IFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITD 240

Query: 241 LSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPG 300
           LSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPG
Sbjct: 241 LSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPG 300

Query: 301 GARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIA 360
           GARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIA
Sbjct: 301 GARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIA 360

Query: 361 IALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLK 420
           IALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLK
Sbjct: 361 IALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLK 420

Query: 421 GGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEAL 480
           GGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEAL
Sbjct: 421 GGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEAL 480

Query: 481 AVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLL 540
           AVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLL
Sbjct: 481 AVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLL 540

Query: 541 KASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRV 600
           KASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRV
Sbjct: 541 KASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRV 600

Query: 601 SSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGG 660
           SSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGG
Sbjct: 601 SSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGG 660

Query: 661 GLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTA 720
           GLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTA
Sbjct: 661 GLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTA 720

Query: 721 LVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDL 780
           LVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDL
Sbjct: 721 LVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDL 780

Query: 781 KQLLMGLKFRGSQPSLDTSSTSTTVQSSLL 811
           KQLLMGLKFRGSQPSLDTSSTSTTVQSSLL
Sbjct: 781 KQLLMGLKFRGSQPSLDTSSTSTTVQSSLL 810

BLAST of Moc02g12230 vs. NCBI nr
Match: XP_022947718.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 719/817 (88.00%), Postives = 752/817 (92.04%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAAD 60
           MGTLSFPI+S K RL  SR+RIAFS P L  S PS AF V N PNLHL RLV C  AA D
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  QSNH--------HHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQ 120
            SNH        HHH HHHHHGHHHCDH  ELT PQRA++GFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKP VKP QNA I +AFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTD 240
           LMALAAFASIFMGN+LEGG+LL MFNLSHIAEEYF SR+MIDVKELKE++PDSALVLDT+
Sbjct: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 DGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           +GKL NI DLSYQKVPVHDVQV+SY+LVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420
           KAVVVLSIA+ALVGP++FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420

Query: 421 CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCC 480
           CARKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+VREN SD G CC
Sbjct: 421 CARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCC 480

Query: 481 IPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTE 540
           IPCCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGLVA L G E
Sbjct: 481 IPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVAMLQGIE 540

Query: 541 SGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIH 600
           SGT GGKLLKASLGSLDFITSL+KS+ KSK+IKEA STSSYGSEFVHAALAVDGKVTLIH
Sbjct: 541 SGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIH 600

Query: 601 LEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHV 660
           LEDRPHP VSS+I EL D+AKLHVMMLTGDHESSA RVAKAVGI EV+FSLKPEDKLSHV
Sbjct: 601 LEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHV 660

Query: 661 KSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720
           KSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC
Sbjct: 661 KSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720

Query: 721 VAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 780
           ++KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCLNSIRALN+
Sbjct: 721 ISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRALNN 780

Query: 781 PSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           PSWSWRQDL QLL+ LK RGS PSL+T   STTV SS
Sbjct: 781 PSWSWRQDLSQLLLKLKLRGSHPSLNT--ISTTVHSS 815

BLAST of Moc02g12230 vs. NCBI nr
Match: XP_023006942.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 717/817 (87.76%), Postives = 754/817 (92.29%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAAD 60
           MGTLSFPI+S K RL  SR+RIAFS P L  S PS AF V N P LHL RLV C  AA D
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPYLHLRRLVLCATAAND 60

Query: 61  QSNH--------HHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQ 120
            SNH        HHH HHHHHGHHHCDH  ELT PQRA++GFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHYHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKP VKP QNA I +AFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTD 240
           LMALAAFASIFMGN+LEGG+LL MFNLSHIAEEYF SR+MIDVKELKE++PDSALVLDT+
Sbjct: 181 LMALAAFASIFMGNALEGGVLLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 DGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           +GKL NI DLSY+KVPVHDVQV+SY+LVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYKKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420
           KAVVVLSIA+ALVGP++FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAY IAISS
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYTIAISS 420

Query: 421 CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCC 480
           CARKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+VREN+SDFG CC
Sbjct: 421 CARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENKSDFGSCC 480

Query: 481 IPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTE 540
           IPCCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGLVATL G E
Sbjct: 481 IPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVATLQGIE 540

Query: 541 SGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIH 600
           SGT GGKLLKASLGSLDFITSL++S+ KSK+IKEA STSSYGSEFVHAALAVDGKVTLIH
Sbjct: 541 SGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIH 600

Query: 601 LEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHV 660
           LEDRPHP VSS+I EL D+AKLHVMMLTGDHESSA RVAKAVGI EV+FSLKPEDKLSHV
Sbjct: 601 LEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHV 660

Query: 661 KSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720
           KSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC
Sbjct: 661 KSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720

Query: 721 VAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 780
           ++KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCLNSIR+LN+
Sbjct: 721 ISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRSLNN 780

Query: 781 PSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           PSWSWRQD  QLL+ LKFRGSQPSL+T   STTV SS
Sbjct: 781 PSWSWRQDFSQLLLKLKFRGSQPSLNT--ISTTVHSS 815

BLAST of Moc02g12230 vs. NCBI nr
Match: XP_023533519.1 (LOW QUALITY PROTEIN: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1367.8 bits (3539), Expect = 0.0e+00
Identity = 713/817 (87.27%), Postives = 751/817 (91.92%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAAD 60
           MGTLSFPI+S K RL  SR+RIAFS P L  S PS AF V N PNLHL RLV C  A  D
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAVND 60

Query: 61  QSNH------HHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLC 120
            SNH      H+HHHHHHHGHHHCDH  ELT PQRA++GFAKAIRWTDLANYLREHLQLC
Sbjct: 61  HSNHEEHHRVHNHHHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQLC 120

Query: 121 CCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLM 180
           CCSMALFVAAAVCPYLVPK  VKP QNA I +AFPLVGVSSSLDALTDISGGKVNIHVLM
Sbjct: 121 CCSMALFVAAAVCPYLVPKLFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHVLM 180

Query: 181 ALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDG 240
           ALAAFASIFMGN+LEGG+LL MFNLSHIAEEYF SR+MIDVKELKE++PDSALVLDT++G
Sbjct: 181 ALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTNEG 240

Query: 241 KLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEIT 300
           KL NI DLSYQKVPVHDVQV+SY+LVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEIT
Sbjct: 241 KLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEIT 300

Query: 301 VGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKA 360
           VGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKA
Sbjct: 301 VGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKA 360

Query: 361 VVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCA 420
           VVVLSIA+ALVGP++FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCA
Sbjct: 361 VVVLSIAVALVGPMLFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCA 420

Query: 421 RKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIP 480
           RKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+VREN+SDFG CCIP
Sbjct: 421 RKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENKSDFGSCCIP 480

Query: 481 CCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESG 540
           CCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGLVA L G ESG
Sbjct: 481 CCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVAMLQGIESG 540

Query: 541 TRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLE 600
           T GGKLLKASLGSLDFITSL++S+ KSK+IKEA STSSYGSEFVHAALAVDGKVTLIHLE
Sbjct: 541 TEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIHLE 600

Query: 601 DRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKS 660
           DRPHP VSS+I EL D+AKLHVMMLTGDHESSA RVAKAVGI EV+FSLKPEDKLSHVKS
Sbjct: 601 DRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHVKS 660

Query: 661 ISRENGGGL--IMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720
           ISRE+G  +   MVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC
Sbjct: 661 ISREHGXQMTFXMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720

Query: 721 VAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 780
           ++KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCLNSIRALN+
Sbjct: 721 ISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRALNN 780

Query: 781 PSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           PSWSWRQ+  QLL+ LKFRGSQPSL+T   STTV SS
Sbjct: 781 PSWSWRQNFSQLLLKLKFRGSQPSLNT--ISTTVHSS 815

BLAST of Moc02g12230 vs. NCBI nr
Match: XP_038900941.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900942.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900943.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 720/817 (88.13%), Postives = 753/817 (92.17%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNR-IAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAA 60
           MGTLSFPI+S KSRL  + N  IAFS PFL +S  S  FR  N P LHL R V C+AA  
Sbjct: 1   MGTLSFPIASSKSRLSSTPNHPIAFSNPFLQSSFSSSPFRPPNFPILHLRRRVLCSAAVT 60

Query: 61  DQSN-------HHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQ 120
            +SN       H HHHHHH H HHHCDH VEL+ PQ+AV+GFAKAIR TDLANYLREHLQ
Sbjct: 61  GRSNHDDHQHVHDHHHHHHRHAHHHCDHDVELSGPQKAVIGFAKAIRLTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPY VPKPIVKPLQNAFIV+AFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYFVPKPIVKPLQNAFIVIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTD 240
           LMALAAFASIFMGN+LEGGLLLAMFNLSHIAEE+FTSR+MIDVKELKEN+PDSALVLDT+
Sbjct: 181 LMALAAFASIFMGNALEGGLLLAMFNLSHIAEEHFTSRAMIDVKELKENYPDSALVLDTN 240

Query: 241 DGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           DGKL N TDLSYQKVPVHDVQV+SYILVGAGE VPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 DGKLPNSTDLSYQKVPVHDVQVDSYILVGAGEFVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVG RVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGSRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420
           KAVVVL+IAIALVGP++FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS
Sbjct: 361 KAVVVLAIAIALVGPVLFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420

Query: 421 CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCC 480
           CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGH+V EN+S+FG CC
Sbjct: 421 CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHEVGENKSEFGSCC 480

Query: 481 IPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTE 540
            PCCE EALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGL+ATL G+E
Sbjct: 481 NPCCEEEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLIATLHGSE 540

Query: 541 SGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIH 600
           SGT G K LKASLGSLDFITS ++SE KS+EIKEA +TSSYGSEFVHAALAVDGKVTLIH
Sbjct: 541 SGTEGRKRLKASLGSLDFITSCYQSESKSREIKEAANTSSYGSEFVHAALAVDGKVTLIH 600

Query: 601 LEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHV 660
           LEDRPHP VSSVI EL D AKLHVMMLTGDHESSA RVA AVGI EVHFSLKPEDKLSHV
Sbjct: 601 LEDRPHPGVSSVIAELTDRAKLHVMMLTGDHESSARRVANAVGIKEVHFSLKPEDKLSHV 660

Query: 661 KSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720
           KSISREN GGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLL DNISGVPFC
Sbjct: 661 KSISREN-GGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLWDNISGVPFC 720

Query: 721 VAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 780
           +AKSRQTTALVKQNASLALFSIF+ASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALN+
Sbjct: 721 IAKSRQTTALVKQNASLALFSIFVASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNN 780

Query: 781 PSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           PSWSW+QDLKQLLM LKF+GSQP L+TS  STTVQSS
Sbjct: 781 PSWSWKQDLKQLLMELKFKGSQPRLNTS--STTVQSS 814

BLAST of Moc02g12230 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 577/807 (71.50%), Postives = 669/807 (82.90%), Query Frame = 0

Query: 5   SFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQSNHH 64
           SF I  PK+ L     RI+ S      +LP  + R+R + + H           D    H
Sbjct: 31  SFSILPPKTLLRQKPLRISAS-----LNLPPRSIRLRAVEDHH------HDHHHDDEQDH 90

Query: 65  HHHHHHHHGHHHCDHGVELTA---PQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALFV 124
           H+HHHHHH H  C   VEL A   PQ+ + GFAKAI W  LANYLREHL LCC + A+F+
Sbjct: 91  HNHHHHHHQHGCC--SVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFL 150

Query: 125 AAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFASI 184
           AAAVCPYL P+P +K LQNAF++V FPLVGVS+SLDAL DI+GGKVNIHVLMALAAFAS+
Sbjct: 151 AAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASV 210

Query: 185 FMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITDL 244
           FMGN+LEGGLLLAMFNL+HIAEE+FTSRSM+DVKELKE++PDSAL+++  +G + NI+DL
Sbjct: 211 FMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDL 270

Query: 245 SYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGG 304
           SY+ VPVH V+V SY+LVG GE VPVDCEV+QGSAT+TIEHLTGE+KPLE   GDRVPGG
Sbjct: 271 SYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGG 330

Query: 305 ARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAI 364
           ARNLDGR+IVKATK W +STL++IV LTEEA  NKP+LQRWLDEFGE+YSK VVVLS+AI
Sbjct: 331 ARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAI 390

Query: 365 ALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLKG 424
           A +GP +FKWPF+ T+ACRGSVYRALGLMVAASPCALAVAPLAYA AISSCARKGILLKG
Sbjct: 391 AFLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKG 450

Query: 425 GRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEALA 484
            +VLDALASCHTIAFDKTGTLTTGGL  KAIEPIYGH+   N S    CCIP CE EALA
Sbjct: 451 AQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQGGTNSSVI-TCCIPNCEKEALA 510

Query: 485 VAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLLK 544
           VAAAMEKGTTHPIGRAVVDHS+GKDLPS  V++ EYFPGRGL AT++G ++     +L K
Sbjct: 511 VAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRK 570

Query: 545 ASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRVS 604
           ASLGS++FITSL KSE +SK+IK+AV+ SSYG +FVHAAL+VD KVTLIHLED+P P VS
Sbjct: 571 ASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVS 630

Query: 605 SVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGGG 664
            VI EL+  A+L VMMLTGDH+SSAWRVA AVGI EV+ +LKPEDKL+HVK+I+RE GGG
Sbjct: 631 GVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGG 690

Query: 665 LIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTAL 724
           LIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD+LLLRDNI+GVPFCVAKSRQTT+L
Sbjct: 691 LIMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSL 750

Query: 725 VKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDLK 784
           VKQN +LAL SIFLA+LP+VLGF+PLWLTVLLHEGGTLLVCLNS+R LNDPSWSW+QD+ 
Sbjct: 751 VKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIV 810

Query: 785 QLLMGLKFRGSQPSLDTSSTSTTVQSS 809
            L+   K R  +P   TSS+S ++ S+
Sbjct: 811 HLIN--KLRSQEP---TSSSSNSLSSA 818

BLAST of Moc02g12230 vs. ExPASy Swiss-Prot
Match: Q60048 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.9e-76
Identity = 201/683 (29.43%), Postives = 351/683 (51.39%), Query Frame = 0

Query: 93  FAKAIRWTDLANYLREHLQLCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGV 152
           F     +TD  +++R++ +L      LF+A      ++       L NA  + A  + G 
Sbjct: 72  FTDPEHFTDHQSFIRKNWRLLL--SGLFIAVGYASQIMNGEDFY-LTNALFIFAIFIGGY 131

Query: 153 SSSLDALTDISGGKVNIHVLMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMI 212
           S   +   ++   +  +  LM +A   + F+G   EG +++ +F +S   E Y   ++  
Sbjct: 132 SLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFAVSEALERYSMDKARQ 191

Query: 213 DVKELKENHPDSALVLDTDDGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVF 272
            ++ L +  P  ALV  +   ++          V V D+Q+   +++  G+ + +D  V 
Sbjct: 192 SIRSLMDIAPKEALVRRSGTDRM----------VHVDDIQIGDIMIIKPGQKIAMDGHVV 251

Query: 273 QGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEA 332
           +G + V    +TGE  P+E  + D V  G  N +G + V  TK  +++T+S+I++L EEA
Sbjct: 252 KGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRVEDTTISKIIHLVEEA 311

Query: 333 QLNKPRLQRWLDEFGEHYSKAVVVLSIAIALVGPIMF--KWPFIGTSACRGSVYRALGLM 392
           Q  +   Q ++D F ++Y+ A++V++  IA V P++F   W           VY+ L ++
Sbjct: 312 QGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLFGGNW--------ETWVYQGLSVL 371

Query: 393 VAASPCALAVA-PLAYAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTG-GLI 452
           V   PCAL V+ P+A   AI + A+ G+L+KGG  L+ +     IAFDKTGTLT G  ++
Sbjct: 372 VVGCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAFDKTGTLTKGVPVV 431

Query: 453 FKAIEPIYGHKVRENRSDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGK--D 512
              IE      ++ N++                + AA+E+ + HP+  A++ +   +  D
Sbjct: 432 TDYIELTEATNIQHNKN--------------YIIMAALEQLSQHPLASAIIKYGETREMD 491

Query: 513 LPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEA 572
           L S +V +     G+G+  T+DG         L K  L S  F  S+H+ ++   ++K  
Sbjct: 492 LTSINVNDFTSITGKGIRGTVDGNTYYVGSPVLFKELLAS-QFTDSIHR-QVSDLQLK-G 551

Query: 573 VSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSA 632
            +   +G+            ++++ + D        VI  L ++     +MLTGD++++A
Sbjct: 552 KTAMLFGTN--------QKLISIVAVADEVRSSSQHVIKRLHELGIEKTIMLTGDNQATA 611

Query: 633 WRVAKAVGINEVHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQ 692
             + + VG++E+   L P+DKL ++K + + N G + MVG+GINDAPALAAATVGI +  
Sbjct: 612 QAIGQQVGVSEIEGELMPQDKLDYIKQL-KINFGKVAMVGDGINDAPALAAATVGIAMGG 671

Query: 693 RASATAIAVADVLLLRDNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFLP 752
             + TAI  ADV L+ D++  +PF V  SR+T  ++KQN + +L    +A L  + G+L 
Sbjct: 672 AGTDTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKLIALLLVIPGWLT 707

Query: 753 LWLTVLLHEGGTLLVCLNSIRAL 770
           LW+ ++   G TLLV LN +R +
Sbjct: 732 LWIAIMADMGATLLVTLNGLRLM 707

BLAST of Moc02g12230 vs. ExPASy Swiss-Prot
Match: P58414 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=cadA PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.2e-73
Identity = 181/629 (28.78%), Postives = 325/629 (51.67%), Query Frame = 0

Query: 144 VVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFASIFMGNSLEGGLLLAMFNLSHIAE 203
           V+A  + G +   +   ++      +  LM +A   +  +G   EG +++ +F  S + E
Sbjct: 120 VIAIVVGGFNLFKEGFANLIKLDFTMESLMTIAIIGASIIGEWAEGSIVVILFAFSEVLE 179

Query: 204 EYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITDLSYQKVPVHDVQVNSYILVGAGE 263
            Y   ++   ++ L +  P  AL+   D            Q + V D+Q+   +++  G+
Sbjct: 180 RYSMDKARQSIRSLMDIAPKEALIRRDD----------VEQMIAVSDIQIGDIMIIKPGQ 239

Query: 264 SVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLS 323
            + +D  V +G + +    +TGE  P+E  V D V  G  N +G + VK TK  +++T+S
Sbjct: 240 KIAMDGVVIKGYSAINQSAITGESIPVEKKVDDEVFAGTLNEEGLLEVKVTKHVEDTTIS 299

Query: 324 RIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAIALVGPIMFKWPFIGTSACRGSV 383
           +I++L EEAQ  +   Q ++D+F ++Y+  ++++++ + +V P+ F   +         V
Sbjct: 300 KIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMLIALLVVVVPPLFFGGDW------DTWV 359

Query: 384 YRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTL 443
           Y+ L L+V   PC+L ++ P++   AI + A+ G+L+KGG  L+ +     IAFDKTGTL
Sbjct: 360 YQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLQAIAFDKTGTL 419

Query: 444 TTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHS 503
           T G       +P+         +DF P      E  +L++  A+E  + HP+  A++  +
Sbjct: 420 TKG-------KPVV--------TDFIPYSEHMDEQNSLSIITALETMSQHPLASAIISKA 479

Query: 504 M--GKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKS 563
           M    D  S  + N     G+G+   ++G        KL ++SL     I+  ++S    
Sbjct: 480 MIDNVDYKSIEIDNFSSITGKGVKGEVNGITYYIGSSKLFESSLEKSQSISQTYQS---- 539

Query: 564 KEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTG 623
              K+  +   +G+E           + +I + D        VI +L  +   H +MLTG
Sbjct: 540 -LQKQGKTAMLFGTE--------SNILAIIAVADEVRESSKEVIAQLHKLGIAHTIMLTG 599

Query: 624 DHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATV 683
           D+  +A  + K +G++++   L PEDKL+++K + ++  G + M+G+G+NDAPALAA+TV
Sbjct: 600 DNNDTAQFIGKEIGVSDIKAELMPEDKLTYIKEL-KQTYGKVAMIGDGVNDAPALAASTV 659

Query: 684 GIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPA 743
           GI +    + TA+  ADV L+ D++  +PF V  SR+T  ++KQN + +L    LA L  
Sbjct: 660 GIAMGGAGTDTALETADVALMGDDLKKLPFIVNLSRKTLKIIKQNITFSLGIKLLALLLV 703

Query: 744 VLGFLPLWLTVLLHEGGTLLVCLNSIRAL 770
           + G+L LW+ ++   G TLLV LN +R +
Sbjct: 720 LPGWLTLWIAIVADMGATLLVTLNGLRLM 703

BLAST of Moc02g12230 vs. ExPASy Swiss-Prot
Match: Q6GIX1 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=cadA PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.2e-69
Identity = 181/608 (29.77%), Postives = 315/608 (51.81%), Query Frame = 0

Query: 168 NIHVLMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALV 227
           ++  LM +A   +  +G   E  +++ +F +S   E +   R+   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 228 LDTDDGKLSNITDLSYQKVPVH--DVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTG 287
           +               Q++ +H  D+ V   ++V  GE + +D  +  G + V    +TG
Sbjct: 220 MRNG------------QEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITG 279

Query: 288 EIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDE 347
           E  P+  TV D V  G  N +G + VK TK  +++T+S+I++L EEAQ  +   Q ++D+
Sbjct: 280 ESVPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDK 339

Query: 348 FGEHYSKAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVA-PLA 407
           F ++Y+  ++V++  +A+V P+ F        +    VY+ L ++V   PCAL ++ P++
Sbjct: 340 FAKYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPIS 399

Query: 408 YAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENR 467
              AI + A+KG+L+KGG  L+ L +   IAFDKTGTLT      K +  +   KV  ++
Sbjct: 400 IVSAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFKVLNDQ 459

Query: 468 SDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDL--PSFSVQNLEYFPGRG 527
            +         E E  ++  A+E  + HP+  A++  +   ++      V++     GRG
Sbjct: 460 VE---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRG 519

Query: 528 LVATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALA 587
           +   +DGT       +L K  L   DF       E K K ++    T+          + 
Sbjct: 520 IQGNIDGTTYYIGSPRLFK-ELNVSDFSLEF---ENKVKVLQNQGKTA--------MIIG 579

Query: 588 VDGKVT-LIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFS 647
            D  +  +I + D       +VI +L  +     +MLTGD++ +A  +   VG++++   
Sbjct: 580 TDQTILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSE 639

Query: 648 LKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLL 707
           L P+DKL ++K +  E+ G + M+G+G+NDAPALAA+TVGI +    + TAI  AD+ L+
Sbjct: 640 LMPQDKLDYIKKMKAEH-GNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALM 699

Query: 708 RDNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLV 767
            D++S +PF V  SR+T  ++K N + A+    +A L  + G+L LW+ +L   G T+LV
Sbjct: 700 GDDLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILV 721

Query: 768 CLNSIRAL 770
            LNS+R +
Sbjct: 760 ALNSLRLM 721

BLAST of Moc02g12230 vs. ExPASy Swiss-Prot
Match: P37386 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.7e-69
Identity = 181/608 (29.77%), Postives = 314/608 (51.64%), Query Frame = 0

Query: 168 NIHVLMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALV 227
           ++  LM +A   +  +G   E  +++ +F +S   E +   R+   ++ L +  P  ALV
Sbjct: 238 DMKTLMTVAVIGAAIIGEWAEASIVVILFAISEALERFSMDRARQSIRSLMDIAPKEALV 297

Query: 228 LDTDDGKLSNITDLSYQKVPVH--DVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTG 287
                           Q++ +H  D+ V   ++V  GE + +D  +  G + V    +TG
Sbjct: 298 RRNG------------QEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITG 357

Query: 288 EIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDE 347
           E  P+  TV D V  G  N +G + VK TK  +++T+S+I++L EEAQ  +   Q ++D+
Sbjct: 358 ESVPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDK 417

Query: 348 FGEHYSKAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAV-APLA 407
           F ++Y+  ++V++  +A+V P+ F        +    VY+ L ++V   PCAL +  P++
Sbjct: 418 FAKYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVITTPIS 477

Query: 408 YAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENR 467
              AI + A+KG+L+KGG  L+ L +   IAFDKTGTLT      K +  +   KV  ++
Sbjct: 478 IVSAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFKVLNDQ 537

Query: 468 SDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDL--PSFSVQNLEYFPGRG 527
            +         E E  ++  A+E  + HP+  A++  +   ++      V++     GRG
Sbjct: 538 VE---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVKDFTSITGRG 597

Query: 528 LVATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALA 587
           +   +DGT       +L K  L   DF       E K K ++    T+          + 
Sbjct: 598 IQGNIDGTTYYIGSPRLFK-ELNVSDFSLEF---ENKVKVLQNQGKTA--------MIIG 657

Query: 588 VDGKVT-LIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFS 647
            D  +  +I + D       +VI++L  +     +MLTGD++ +A  +   VG++++   
Sbjct: 658 TDQTILGVIAVADEVRETSKNVILKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSE 717

Query: 648 LKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLL 707
           L P+DKL ++K +  E+ G + M+G+G+NDAPALAA+TVGI +    + TAI  AD+ L+
Sbjct: 718 LLPQDKLDYIKKMKAEH-GNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALM 777

Query: 708 RDNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLV 767
            D++S +PF V  SR+T  ++K N + A+    +A L  + G+L LW+ +L   G T+LV
Sbjct: 778 GDDLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILV 799

Query: 768 CLNSIRAL 770
            LNS+R +
Sbjct: 838 ALNSLRLM 799

BLAST of Moc02g12230 vs. ExPASy TrEMBL
Match: A0A6J1D720 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111017943 PE=3 SV=1)

HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 810/810 (100.00%), Postives = 810/810 (100.00%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQ 60
           MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQ
Sbjct: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQ 60

Query: 61  SNHHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALF 120
           SNHHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALF
Sbjct: 61  SNHHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALF 120

Query: 121 VAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFAS 180
           VAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFAS
Sbjct: 121 VAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFAS 180

Query: 181 IFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITD 240
           IFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITD
Sbjct: 181 IFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITD 240

Query: 241 LSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPG 300
           LSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPG
Sbjct: 241 LSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPG 300

Query: 301 GARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIA 360
           GARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIA
Sbjct: 301 GARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIA 360

Query: 361 IALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLK 420
           IALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLK
Sbjct: 361 IALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLK 420

Query: 421 GGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEAL 480
           GGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEAL
Sbjct: 421 GGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEAL 480

Query: 481 AVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLL 540
           AVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLL
Sbjct: 481 AVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLL 540

Query: 541 KASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRV 600
           KASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRV
Sbjct: 541 KASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRV 600

Query: 601 SSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGG 660
           SSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGG
Sbjct: 601 SSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGG 660

Query: 661 GLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTA 720
           GLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTA
Sbjct: 661 GLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTA 720

Query: 721 LVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDL 780
           LVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDL
Sbjct: 721 LVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDL 780

Query: 781 KQLLMGLKFRGSQPSLDTSSTSTTVQSSLL 811
           KQLLMGLKFRGSQPSLDTSSTSTTVQSSLL
Sbjct: 781 KQLLMGLKFRGSQPSLDTSSTSTTVQSSLL 810

BLAST of Moc02g12230 vs. ExPASy TrEMBL
Match: A0A6J1G7D7 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451500 PE=3 SV=1)

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 719/817 (88.00%), Postives = 752/817 (92.04%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAAD 60
           MGTLSFPI+S K RL  SR+RIAFS P L  S PS AF V N PNLHL RLV C  AA D
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  QSNH--------HHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQ 120
            SNH        HHH HHHHHGHHHCDH  ELT PQRA++GFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKP VKP QNA I +AFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTD 240
           LMALAAFASIFMGN+LEGG+LL MFNLSHIAEEYF SR+MIDVKELKE++PDSALVLDT+
Sbjct: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 DGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           +GKL NI DLSYQKVPVHDVQV+SY+LVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420
           KAVVVLSIA+ALVGP++FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420

Query: 421 CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCC 480
           CARKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+VREN SD G CC
Sbjct: 421 CARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCC 480

Query: 481 IPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTE 540
           IPCCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGLVA L G E
Sbjct: 481 IPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVAMLQGIE 540

Query: 541 SGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIH 600
           SGT GGKLLKASLGSLDFITSL+KS+ KSK+IKEA STSSYGSEFVHAALAVDGKVTLIH
Sbjct: 541 SGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIH 600

Query: 601 LEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHV 660
           LEDRPHP VSS+I EL D+AKLHVMMLTGDHESSA RVAKAVGI EV+FSLKPEDKLSHV
Sbjct: 601 LEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHV 660

Query: 661 KSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720
           KSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC
Sbjct: 661 KSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720

Query: 721 VAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 780
           ++KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCLNSIRALN+
Sbjct: 721 ISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRALNN 780

Query: 781 PSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           PSWSWRQDL QLL+ LK RGS PSL+T   STTV SS
Sbjct: 781 PSWSWRQDLSQLLLKLKLRGSHPSLNT--ISTTVHSS 815

BLAST of Moc02g12230 vs. ExPASy TrEMBL
Match: A0A6J1KX68 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499582 PE=3 SV=1)

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 717/817 (87.76%), Postives = 754/817 (92.29%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAAD 60
           MGTLSFPI+S K RL  SR+RIAFS P L  S PS AF V N P LHL RLV C  AA D
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPYLHLRRLVLCATAAND 60

Query: 61  QSNH--------HHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQ 120
            SNH        HHH HHHHHGHHHCDH  ELT PQRA++GFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHYHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKP VKP QNA I +AFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTD 240
           LMALAAFASIFMGN+LEGG+LL MFNLSHIAEEYF SR+MIDVKELKE++PDSALVLDT+
Sbjct: 181 LMALAAFASIFMGNALEGGVLLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 DGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           +GKL NI DLSY+KVPVHDVQV+SY+LVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYKKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISS 420
           KAVVVLSIA+ALVGP++FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAY IAISS
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYTIAISS 420

Query: 421 CARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCC 480
           CARKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+VREN+SDFG CC
Sbjct: 421 CARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENKSDFGSCC 480

Query: 481 IPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTE 540
           IPCCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGLVATL G E
Sbjct: 481 IPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVATLQGIE 540

Query: 541 SGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIH 600
           SGT GGKLLKASLGSLDFITSL++S+ KSK+IKEA STSSYGSEFVHAALAVDGKVTLIH
Sbjct: 541 SGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIH 600

Query: 601 LEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHV 660
           LEDRPHP VSS+I EL D+AKLHVMMLTGDHESSA RVAKAVGI EV+FSLKPEDKLSHV
Sbjct: 601 LEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHV 660

Query: 661 KSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720
           KSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC
Sbjct: 661 KSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFC 720

Query: 721 VAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 780
           ++KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCLNSIR+LN+
Sbjct: 721 ISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRSLNN 780

Query: 781 PSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           PSWSWRQD  QLL+ LKFRGSQPSL+T   STTV SS
Sbjct: 781 PSWSWRQDFSQLLLKLKFRGSQPSLNT--ISTTVHSS 815

BLAST of Moc02g12230 vs. ExPASy TrEMBL
Match: A0A5A7VJB5 (Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G005290 PE=3 SV=1)

HSP 1 Score: 1330.9 bits (3443), Expect = 0.0e+00
Identity = 711/828 (85.87%), Postives = 743/828 (89.73%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRI-AFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAA 60
           MGTLSFPI S KS L  + N +  F  PFL +S  S  F  RN P LHL R V C+AAAA
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILHLRRRVLCSAAAA 60

Query: 61  DQSN----------------HHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDL 120
            +SN                HHHHHHHHHH HHH DH VEL+ PQ+AV+GFAKA RWTDL
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHHHHHHGDHDVELSGPQKAVIGFAKATRWTDL 120

Query: 121 ANYLREHLQLCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDI 180
           ANYLRE LQLCCCSMALFVAAAVCPYLVPKPIVKPLQ AFIV+ FPLVGVSS+LDALTDI
Sbjct: 121 ANYLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVGVSSALDALTDI 180

Query: 181 SGGKVNIHVLMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHP 240
           SGGKVNIHVLMALAAFASIFMGN+LEGGLLLAMFNLSHIAEEYFTSR+MIDVKELKEN+P
Sbjct: 181 SGGKVNIHVLMALAAFASIFMGNALEGGLLLAMFNLSHIAEEYFTSRAMIDVKELKENYP 240

Query: 241 DSALVLDTDDGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEH 300
           D ALVLDT+DG++ NITDLSYQKVPV DV+V+SYILVGAGE VPVDCEVFQGSATVTIEH
Sbjct: 241 DFALVLDTNDGEVPNITDLSYQKVPVQDVEVDSYILVGAGEFVPVDCEVFQGSATVTIEH 300

Query: 301 LTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRW 360
           LTGEIKPLEITVG RVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRW
Sbjct: 301 LTGEIKPLEITVGSRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRW 360

Query: 361 LDEFGEHYSKAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAP 420
           LDEFGEHYSKAVVVL+  IALVGPI+FKWPFIGTSACRGSVYRALGLMVAASPCALAVAP
Sbjct: 361 LDEFGEHYSKAVVVLATTIALVGPILFKWPFIGTSACRGSVYRALGLMVAASPCALAVAP 420

Query: 421 LAYAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRE 480
           LAYAIAISSCARKGILLKGGRVLDALA+CHTIAFDKTGTLTTGGL FKAIEPIYGH+V E
Sbjct: 421 LAYAIAISSCARKGILLKGGRVLDALAACHTIAFDKTGTLTTGGLFFKAIEPIYGHEVGE 480

Query: 481 NRSDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRG 540
           N+SDFG CCIPCCE EALAVAAAMEKGTTHPIGRAVV HSMGK LPSFSVQ+LEYFPGRG
Sbjct: 481 NKSDFGSCCIPCCEDEALAVAAAMEKGTTHPIGRAVVGHSMGKVLPSFSVQSLEYFPGRG 540

Query: 541 LVATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALA 600
           LVATL GTES   GGKLLKASLGSLDFITS +KS  KSKEIKEA +TSSYGSEFVHAALA
Sbjct: 541 LVATLHGTES---GGKLLKASLGSLDFITSCYKSGTKSKEIKEAANTSSYGSEFVHAALA 600

Query: 601 VDGKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSL 660
           VDGKVTLIHLEDRPHP VSSVI EL D A+LHVMMLTGDHESSA RVA AVGI EVHFSL
Sbjct: 601 VDGKVTLIHLEDRPHPGVSSVISELTDRARLHVMMLTGDHESSARRVANAVGIKEVHFSL 660

Query: 661 KPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLR 720
           KPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLR
Sbjct: 661 KPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLR 720

Query: 721 DNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVC 780
           DNISGVPFC+AKSRQTTALVKQNASLALF IF+ASLPAVLGFLPLWLTVLLHEGGTLLVC
Sbjct: 721 DNISGVPFCIAKSRQTTALVKQNASLALFFIFVASLPAVLGFLPLWLTVLLHEGGTLLVC 780

Query: 781 LNSIRALNDPSWSWR--QDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           LNSIRALN+PSWSW   QDLKQ LM LKF+GSQP L+TS  STTVQSS
Sbjct: 781 LNSIRALNNPSWSWSWGQDLKQFLMELKFKGSQPRLNTS--STTVQSS 823

BLAST of Moc02g12230 vs. ExPASy TrEMBL
Match: A0A5D3BGM3 (Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G001560 PE=3 SV=1)

HSP 1 Score: 1323.1 bits (3423), Expect = 0.0e+00
Identity = 708/826 (85.71%), Postives = 740/826 (89.59%), Query Frame = 0

Query: 1   MGTLSFPISSPKSRLPLSRNRI-AFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAA 60
           MGTLSFPI S KS L  + N +  F  PFL +S  S  F  RN P L L R V C+AAAA
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILRLRRRVLCSAAAA 60

Query: 61  DQSN--------------HHHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLAN 120
            +SN              HHHHHHHHHH  HH DH VEL+ PQ+AV+GFAKA RWTDLA 
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHRHHGDHDVELSGPQKAVIGFAKATRWTDLAK 120

Query: 121 YLREHLQLCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISG 180
           YLRE LQLCCCSMALFVAAAVCPYLVPKPIVKPLQ AFIV+ FPLVGVSS+LDALTDISG
Sbjct: 121 YLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVGVSSALDALTDISG 180

Query: 181 GKVNIHVLMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDS 240
           GKVNIHVLMALAAFASIFMGN+LEGGLLLAMFNLSHIAEEYFTSR+MIDVKELKEN+PD 
Sbjct: 181 GKVNIHVLMALAAFASIFMGNALEGGLLLAMFNLSHIAEEYFTSRAMIDVKELKENYPDF 240

Query: 241 ALVLDTDDGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLT 300
           ALVLDT+DG++ NITDLSYQKVPV DV+V+SYILVGAGE VPVDCEVFQGSATVTIEHLT
Sbjct: 241 ALVLDTNDGEVPNITDLSYQKVPVQDVEVDSYILVGAGEFVPVDCEVFQGSATVTIEHLT 300

Query: 301 GEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD 360
           GEIKPLEITVG RVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD
Sbjct: 301 GEIKPLEITVGSRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD 360

Query: 361 EFGEHYSKAVVVLSIAIALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLA 420
           EFGEHYSKAVVVL+  IALVGPI+FKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 EFGEHYSKAVVVLATTIALVGPILFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENR 480
           YAIAISSCARKGILLKGGRVLDALA+CHTIAFDKTGTLTTGGL FKAIEPIYGH+V EN+
Sbjct: 421 YAIAISSCARKGILLKGGRVLDALAACHTIAFDKTGTLTTGGLFFKAIEPIYGHEVGENK 480

Query: 481 SDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLV 540
           SDFG CCIPCCE EALAVAAAMEKGTTHPIGRAVV HSMGK LPSFSVQ+LEYFPGRGLV
Sbjct: 481 SDFGSCCIPCCEDEALAVAAAMEKGTTHPIGRAVVGHSMGKVLPSFSVQSLEYFPGRGLV 540

Query: 541 ATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVD 600
           ATL GTES   GGKLLKASLGSLDFITS +KS  KSKEIKEA +TSSYGSEFVHAALAVD
Sbjct: 541 ATLHGTES---GGKLLKASLGSLDFITSCYKSGTKSKEIKEAANTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKP 660
           GKVTLIHLEDRPHP VSSVI EL D A+LHVMMLTGDHESSA RVA AVGI EVHFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSVISELTDRARLHVMMLTGDHESSARRVANAVGIKEVHFSLKP 660

Query: 661 EDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFC+AKSRQTTALVKQNASLALF IF+ASLPAVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNASLALFFIFVASLPAVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNDPSWSWR--QDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 809
           SIRALN+PSWSW   QDLKQ LM LKF+GSQP L+TS  STTVQSS
Sbjct: 781 SIRALNNPSWSWSWGQDLKQFLMELKFKGSQPRLNTS--STTVQSS 821

BLAST of Moc02g12230 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 577/807 (71.50%), Postives = 669/807 (82.90%), Query Frame = 0

Query: 5   SFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHLRLVPCTAAAADQSNHH 64
           SF I  PK+ L     RI+ S      +LP  + R+R + + H           D    H
Sbjct: 31  SFSILPPKTLLRQKPLRISAS-----LNLPPRSIRLRAVEDHH------HDHHHDDEQDH 90

Query: 65  HHHHHHHHGHHHCDHGVELTA---PQRAVVGFAKAIRWTDLANYLREHLQLCCCSMALFV 124
           H+HHHHHH H  C   VEL A   PQ+ + GFAKAI W  LANYLREHL LCC + A+F+
Sbjct: 91  HNHHHHHHQHGCC--SVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFL 150

Query: 125 AAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFASI 184
           AAAVCPYL P+P +K LQNAF++V FPLVGVS+SLDAL DI+GGKVNIHVLMALAAFAS+
Sbjct: 151 AAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASV 210

Query: 185 FMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITDL 244
           FMGN+LEGGLLLAMFNL+HIAEE+FTSRSM+DVKELKE++PDSAL+++  +G + NI+DL
Sbjct: 211 FMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDL 270

Query: 245 SYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGG 304
           SY+ VPVH V+V SY+LVG GE VPVDCEV+QGSAT+TIEHLTGE+KPLE   GDRVPGG
Sbjct: 271 SYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGG 330

Query: 305 ARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAI 364
           ARNLDGR+IVKATK W +STL++IV LTEEA  NKP+LQRWLDEFGE+YSK VVVLS+AI
Sbjct: 331 ARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAI 390

Query: 365 ALVGPIMFKWPFIGTSACRGSVYRALGLMVAASPCALAVAPLAYAIAISSCARKGILLKG 424
           A +GP +FKWPF+ T+ACRGSVYRALGLMVAASPCALAVAPLAYA AISSCARKGILLKG
Sbjct: 391 AFLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKG 450

Query: 425 GRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEALA 484
            +VLDALASCHTIAFDKTGTLTTGGL  KAIEPIYGH+   N S    CCIP CE EALA
Sbjct: 451 AQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQGGTNSSVI-TCCIPNCEKEALA 510

Query: 485 VAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLLK 544
           VAAAMEKGTTHPIGRAVVDHS+GKDLPS  V++ EYFPGRGL AT++G ++     +L K
Sbjct: 511 VAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRK 570

Query: 545 ASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRVS 604
           ASLGS++FITSL KSE +SK+IK+AV+ SSYG +FVHAAL+VD KVTLIHLED+P P VS
Sbjct: 571 ASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVS 630

Query: 605 SVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKPEDKLSHVKSISRENGGG 664
            VI EL+  A+L VMMLTGDH+SSAWRVA AVGI EV+ +LKPEDKL+HVK+I+RE GGG
Sbjct: 631 GVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGG 690

Query: 665 LIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTAL 724
           LIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD+LLLRDNI+GVPFCVAKSRQTT+L
Sbjct: 691 LIMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSL 750

Query: 725 VKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALNDPSWSWRQDLK 784
           VKQN +LAL SIFLA+LP+VLGF+PLWLTVLLHEGGTLLVCLNS+R LNDPSWSW+QD+ 
Sbjct: 751 VKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIV 810

Query: 785 QLLMGLKFRGSQPSLDTSSTSTTVQSS 809
            L+   K R  +P   TSS+S ++ S+
Sbjct: 811 HLIN--KLRSQEP---TSSSSNSLSSA 818

BLAST of Moc02g12230 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 206.1 bits (523), Expect = 1.1e-52
Identity = 174/637 (27.32%), Postives = 313/637 (49.14%), Query Frame = 0

Query: 142 FIVVAFPLVGVSSSL-DALTDISGGKVNIHVLMALAAFASIFMGNSLEGGLLLAMFNLSH 201
           ++ VA  + G+   L  A+  ++  +++I++L+ +   A+I M +  E  +++ +F ++ 
Sbjct: 112 WLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAE 171

Query: 202 IAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITDLSYQKVPVHDVQVNSYILVG 261
             +   + ++   ++ L    P  A++ +T             ++V V +++ N+ I V 
Sbjct: 172 WLQSRASYKASAVMQSLMSLAPQKAVIAETG------------EEVEVDELKTNTVIAVK 231

Query: 262 AGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKES 321
           AGE++P+D  V  G+  V  + LTGE  P+       V  G  NL+G I V  T   ++ 
Sbjct: 232 AGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDC 291

Query: 322 TLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAIALVGPIMFKWPF-IGTSAC 381
            ++++  L EEAQ +K   QR++D+  ++Y+ A++++SI    +       PF +     
Sbjct: 292 VVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-------PFALKVHNL 351

Query: 382 RGSVYRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGRVLDALASCHTIAFDK 441
           +  V+ AL ++V+A PC L ++ P+A   A++  A  G+L+KG   L+ LA    +AFDK
Sbjct: 352 KHWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDK 411

Query: 442 TGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAV 501
           TGT+T G  I                 DF            L   ++ E  ++HP+  AV
Sbjct: 412 TGTITRGEFIV---------------MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAV 471

Query: 502 VDH--SMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLLKASLGSLDFITSLHKS 561
           VD+  S+  +    +V++ + FPG G+   +DG E    G K + +  G L    S+   
Sbjct: 472 VDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYI-GNKRIASRAGCL----SVPDI 531

Query: 562 EIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRVSSVIVELRDVAKLHVM 621
           ++ +K  K      + G  +V   LA      + +L D     V+  + EL+ +  + + 
Sbjct: 532 DVDTKGGK------TIGYVYVGETLA-----GVFNLSDACRSGVAQAMKELKSLG-IKIA 591

Query: 622 MLTGDHESSAWRVAKAVG--INEVHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPA 681
           MLTGD+ ++A    + +G  ++ V   L PEDK   +K + RE  G   MVG+G+NDAPA
Sbjct: 592 MLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREE-GPTAMVGDGLNDAPA 651

Query: 682 LAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTALVKQNASLALFSIF 741
           LA A +GI +    SA A    +++L+ ++I  +P  +  +++    V +N  +++    
Sbjct: 652 LATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKG 696

Query: 742 LASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 772
                A  G   +W  VL   G  LLV LNS+  L+D
Sbjct: 712 AILALAFAGHPLIWAAVLADVGTCLLVILNSMLLLSD 696

BLAST of Moc02g12230 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 204.1 bits (518), Expect = 4.1e-52
Identity = 175/646 (27.09%), Postives = 314/646 (48.61%), Query Frame = 0

Query: 132 KPIVKPLQNAFIVVAFPLVGVSSSL-DALTDISGGKVNIHVLMALAAFASIFMGNSLEGG 191
           K +  PL+  ++ VA    G+   L  A   I   +++I++L+ +   A++ M + +E  
Sbjct: 114 KFVYSPLR--WLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAA 173

Query: 192 LLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTDDGKLSNITDLSYQKVPVHD 251
            ++ +F +S   E   + ++   ++ L    P  A++ +T             ++V V +
Sbjct: 174 AVVFLFTISDWLETRASYKATSVMQSLMSLAPQKAIIAETG------------EEVEVDE 233

Query: 252 VQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRII 311
           V+V++ + V AGE++P+D  V  G+  V  + LTGE  P+       V  G  NL+G I 
Sbjct: 234 VKVDTVVAVKAGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYIC 293

Query: 312 VKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAIALVGPIMFK 371
           VK T    +  ++++  L EEAQ +K + QR +D+  ++Y+ A++++S  +A+V P++ K
Sbjct: 294 VKTTSLAGDCVVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMK 353

Query: 372 WPFIGTSACRGSVYRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGRVLDALA 431
                    +   + AL ++V+  PC L ++ P+A   A++  A  G+L+K    LD L+
Sbjct: 354 -----VHNLKHWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLS 413

Query: 432 SCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFGPCCIPCCETEALAVAAAMEKG 491
               +AFDKTGT+T G  I                 DF            L   +++E  
Sbjct: 414 KIKIVAFDKTGTITRGEFIV---------------IDFKSLSRDINLRSLLYWVSSVESK 473

Query: 492 TTHPIGRAVVDH--SMGKDLPSFSVQNLEYFPGRGLVATLDGTESGTRGGKLLKASLGSL 551
           ++HP+   +VD+  S+  +     V++ + FPG G+   +DG +    G K + +  G  
Sbjct: 474 SSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFI-GNKKIASRAG-- 533

Query: 552 DFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVDGKVTLIHLEDRPHPRVSSVIVEL 611
              +++ + E+ +K  K      + G  +V   LA        +L D     VS  + EL
Sbjct: 534 --CSTVPEIEVDTKGGK------TVGYVYVGERLA-----GFFNLSDACRSGVSQAMAEL 593

Query: 612 RDVAKLHVMMLTGDHESSAWRVAKAVG--INEVHFSLKPEDKLSHVKSISRENGGGLIMV 671
           + +  +   MLTGD++++A    + +G  ++ VH  L PEDK   ++   +E  G   MV
Sbjct: 594 KSLG-IKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMV 653

Query: 672 GEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCVAKSRQTTALVKQN 731
           G+G+NDAPALA A +GI +    SA A    +++L+ ++I  +P  V  +R+    V +N
Sbjct: 654 GDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVEN 705

Query: 732 ASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLNSIRALND 772
             L++         A  G   +W  VL+  G  LLV  NS+  L +
Sbjct: 714 VCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLRE 705

BLAST of Moc02g12230 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 191.0 bits (484), Expect = 3.6e-48
Identity = 175/591 (29.61%), Postives = 278/591 (47.04%), Query Frame = 0

Query: 191 LLLAMFNLSHIAEEYFTSRSMIDVKELKE-NHPDSALVLDTDDGKLSNITDLSYQ----K 250
           +LL    L    EE    ++  D+ EL       S LV+ + D      + LS       
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 251 VPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNL 310
           V V D++V   +LV  GE+ PVD  V  G + V    LTGE  P+    G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 311 DGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAIALVG 370
           DG + +KA+ T   ST+S+IV + E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAF- 465

Query: 371 PIMFKWPFIGT----------------SACRGSVYRALGLMVAASPCALAVA-PLAYAIA 430
                W ++G+                 A   S+  A+ ++V + PCAL +A P A  I 
Sbjct: 466 -----WYYVGSHIFPDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIG 525

Query: 431 ISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFG 490
            S  A++G L++GG VL+ LAS   +A DKTGTLT G  +   +  + G++         
Sbjct: 526 TSLGAKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE--------- 585

Query: 491 PCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLD 550
                  E E L +AAA+EK  THPI +A+V+ +   +L +   +     PG G +A +D
Sbjct: 586 -------EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEID 645

Query: 551 GTESGTRGGKLLKASLGSLDFITS--LHKSE---------IKSKEIKEAVSTSSYGSEFV 610
           G             ++GSL++++   L K++         +   ++    STS Y    V
Sbjct: 646 GR----------FVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVV 705

Query: 611 HAALAVDGKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGI-- 670
           +     +G +  I + D         +  L++   +  ++L+GD E +   VAK VGI  
Sbjct: 706 YVGREGEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKS 765

Query: 671 NEVHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIA- 730
              ++SL PE K   + ++ + +G  + MVG+GINDAP+LA A VGI L   A   A + 
Sbjct: 766 ESTNYSLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASN 825

Query: 731 VADVLLLRDNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFL 746
            A V+L+R+ +S V   ++ ++ T + V QN + A+ +  + S+P   G L
Sbjct: 826 AASVILVRNKLSHVVDALSLAQATMSKVYQNLAWAI-AYNVISIPIAAGVL 840

BLAST of Moc02g12230 vs. TAIR 10
Match: AT5G21930.2 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 191.0 bits (484), Expect = 3.6e-48
Identity = 175/591 (29.61%), Postives = 278/591 (47.04%), Query Frame = 0

Query: 191 LLLAMFNLSHIAEEYFTSRSMIDVKELKE-NHPDSALVLDTDDGKLSNITDLSYQ----K 250
           +LL    L    EE    ++  D+ EL       S LV+ + D      + LS       
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 251 VPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNL 310
           V V D++V   +LV  GE+ PVD  V  G + V    LTGE  P+    G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 311 DGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAIALVG 370
           DG + +KA+ T   ST+S+IV + E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAF- 465

Query: 371 PIMFKWPFIGT----------------SACRGSVYRALGLMVAASPCALAVA-PLAYAIA 430
                W ++G+                 A   S+  A+ ++V + PCAL +A P A  I 
Sbjct: 466 -----WYYVGSHIFPDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIG 525

Query: 431 ISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENRSDFG 490
            S  A++G L++GG VL+ LAS   +A DKTGTLT G  +   +  + G++         
Sbjct: 526 TSLGAKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE--------- 585

Query: 491 PCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLVATLD 550
                  E E L +AAA+EK  THPI +A+V+ +   +L +   +     PG G +A +D
Sbjct: 586 -------EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEID 645

Query: 551 GTESGTRGGKLLKASLGSLDFITS--LHKSE---------IKSKEIKEAVSTSSYGSEFV 610
           G             ++GSL++++   L K++         +   ++    STS Y    V
Sbjct: 646 GR----------FVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVV 705

Query: 611 HAALAVDGKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGI-- 670
           +     +G +  I + D         +  L++   +  ++L+GD E +   VAK VGI  
Sbjct: 706 YVGREGEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKS 765

Query: 671 NEVHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIA- 730
              ++SL PE K   + ++ + +G  + MVG+GINDAP+LA A VGI L   A   A + 
Sbjct: 766 ESTNYSLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASN 825

Query: 731 VADVLLLRDNISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFL 746
            A V+L+R+ +S V   ++ ++ T + V QN + A+ +  + S+P   G L
Sbjct: 826 AASVILVRNKLSHVVDALSLAQATMSKVYQNLAWAI-AYNVISIPIAAGVL 840