Moc02g11670 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g11670
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cytochrome P450 89A2-like
Location	chr2: 8391335 .. 8391856 (+)
RNA-Seq Expression	Moc02g11670
Synteny	Moc02g11670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAAGGAGAGGAGGAGGTGAAGGAAGAAGAATTGGAGAAGCTTCCATATCTGAAAGCTGTGGTTTTAGAAGGGTTGAGAAGACACCCACCGGCGCATTTCCTGCTGCCACACGCAGTGAAAGAAGACACGAATTTGGGAAATTACGTGATACCCAAAAATGGGACAGTAAATTTCATGGTGGGGGAGATGGGTCTGGATCCAAAAGTGTGGGAAGATCCAATGGCGTTCAAGCCGGAGAGGTTCATGAGGATGGGCGGCGGCGGGGAAGAAGAAACGGCGGCGTTTGACATAACAGGGAGCAAGGGGATTAAGATGATGCCGTTCGGAGCAGGGAGAAGAATGTGTCCAGGATTTGGGCTGGCGATCCTTCATTTGGAGTATTTCGTTGCGAATTTGGTTTGGCGATTGGAGTGGAAGGCCGTCGACGGAGACGACGTTGATCTGTCGGAGAAGGTAGAATTGACGATCGTGATGAAAAAGCCTCTGAAAGTCAACGTACATCCCAGGAATTTTTAG

mRNA sequence

ATGGGAAAAGGAGAGGAGGAGGTGAAGGAAGAAGAATTGGAGAAGCTTCCATATCTGAAAGCTGTGGTTTTAGAAGGGTTGAGAAGACACCCACCGGCGCATTTCCTGCTGCCACACGCAGTGAAAGAAGACACGAATTTGGGAAATTACGTGATACCCAAAAATGGGACAGTAAATTTCATGGTGGGGGAGATGGGTCTGGATCCAAAAGTGTGGGAAGATCCAATGGCGTTCAAGCCGGAGAGGTTCATGAGGATGGGCGGCGGCGGGGAAGAAGAAACGGCGGCGTTTGACATAACAGGGAGCAAGGGGATTAAGATGATGCCGTTCGGAGCAGGGAGAAGAATGTGTCCAGGATTTGGGCTGGCGATCCTTCATTTGGAGTATTTCGTTGCGAATTTGGTTTGGCGATTGGAGTGGAAGGCCGTCGACGGAGACGACGTTGATCTGTCGGAGAAGGTAGAATTGACGATCGTGATGAAAAAGCCTCTGAAAGTCAACGTACATCCCAGGAATTTTTAG

Coding sequence (CDS)

ATGGGAAAAGGAGAGGAGGAGGTGAAGGAAGAAGAATTGGAGAAGCTTCCATATCTGAAAGCTGTGGTTTTAGAAGGGTTGAGAAGACACCCACCGGCGCATTTCCTGCTGCCACACGCAGTGAAAGAAGACACGAATTTGGGAAATTACGTGATACCCAAAAATGGGACAGTAAATTTCATGGTGGGGGAGATGGGTCTGGATCCAAAAGTGTGGGAAGATCCAATGGCGTTCAAGCCGGAGAGGTTCATGAGGATGGGCGGCGGCGGGGAAGAAGAAACGGCGGCGTTTGACATAACAGGGAGCAAGGGGATTAAGATGATGCCGTTCGGAGCAGGGAGAAGAATGTGTCCAGGATTTGGGCTGGCGATCCTTCATTTGGAGTATTTCGTTGCGAATTTGGTTTGGCGATTGGAGTGGAAGGCCGTCGACGGAGACGACGTTGATCTGTCGGAGAAGGTAGAATTGACGATCGTGATGAAAAAGCCTCTGAAAGTCAACGTACATCCCAGGAATTTTTAG

Protein sequence

MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNFMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGFGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPRNF

Homology

[Close]

BLAST of Moc02g11670 vs. NCBI nr
Match: XP_038902538.1 (cytochrome P450 89A2-like [Benincasa hispida])

HSP 1 Score: 301.2 bits (770), Expect = 5.7e-78
Identity = 145/172 (84.30%), Postives = 156/172 (90.70%), Query Frame = 0


Query: 1   MGKG-EEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG+G +EEVKEE+L KLPYLKAV+LEGLRRHPPAHFLLPHAVKEDT LGNYVIPKNGTVN
Sbjct: 339 MGEGSKEEVKEEDLGKLPYLKAVILEGLRRHPPAHFLLPHAVKEDTELGNYVIPKNGTVN 398

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           FMV EMG DPKVWEDPM FKPERFM+ G G  EE   FDITGSK IKMMPFGAGRRMCPG
Sbjct: 399 FMVAEMGRDPKVWEDPMNFKPERFMKAGEG--EEGGWFDITGSKEIKMMPFGAGRRMCPG 458

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           +GLAILHLEYFVANL+WR EWKA+DGD+VDLSEKVELTIVMKKPL+ N+HPR
Sbjct: 459 YGLAILHLEYFVANLIWRFEWKAIDGDEVDLSEKVELTIVMKKPLQANLHPR 508

[Close]

BLAST of Moc02g11670 vs. NCBI nr
Match: XP_022148533.1 (cytochrome P450 89A2-like [Momordica charantia])

HSP 1 Score: 300.8 bits (769), Expect = 7.4e-78
Identity = 142/171 (83.04%), Postives = 152/171 (88.89%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           MGKGEEEVKEE+L KLPYLKAVVLEGLRRHPP HF+LPHAVKEDT L NY IPKNGTVNF
Sbjct: 348 MGKGEEEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKEDTTLENYAIPKNGTVNF 407

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV +MG DPKVWEDPMAFKPERFM  GGGGE ET AFDITGSK IKMMPFGAGRRMCPG 
Sbjct: 408 MVAQMGWDPKVWEDPMAFKPERFMNGGGGGEGETIAFDITGSKEIKMMPFGAGRRMCPGL 467

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           G+A+LHLEYF+ANLVWR EWKAV GDDVDLSEKVE T+VM+KPLK  ++ R
Sbjct: 468 GVAMLHLEYFIANLVWRFEWKAVAGDDVDLSEKVEFTVVMEKPLKAALNSR 518

[Close]

BLAST of Moc02g11670 vs. NCBI nr
Match: XP_011650074.1 (cytochrome P450 89A2 [Cucumis sativus] >KGN63391.1 hypothetical protein Csa_022467 [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 1.1e-76
Identity = 145/172 (84.30%), Postives = 153/172 (88.95%), Query Frame = 0


Query: 1   MGKG-EEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG G EEEVKEE+L KLPYLKAV+LEGLRRHPPAHFLLPHAVKE+T LGNYVIPKNGT N
Sbjct: 342 MGDGSEEEVKEEDLGKLPYLKAVILEGLRRHPPAHFLLPHAVKEETKLGNYVIPKNGTTN 401

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           +MV EMG DPKVWEDPMAFKPERFM+    G EE   FDITGSK IKMMPFGAGRRMCPG
Sbjct: 402 YMVAEMGRDPKVWEDPMAFKPERFMK----GGEEGIEFDITGSKEIKMMPFGAGRRMCPG 461

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           FGLAILHLEYFVANLVWR EWKAVDGD+VDLSEKVELTIVMKKPL+ N+ PR
Sbjct: 462 FGLAILHLEYFVANLVWRFEWKAVDGDEVDLSEKVELTIVMKKPLQANLRPR 509

[Close]

BLAST of Moc02g11670 vs. NCBI nr
Match: XP_022943745.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 294.3 bits (752), Expect = 7.0e-76
Identity = 142/172 (82.56%), Postives = 152/172 (88.37%), Query Frame = 0


Query: 1   MGKGE-EEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG G  EEVKEE+L KLPYL+AVVLEGLRRHPP HF+LPHAVKEDT L NYVIPKNG+VN
Sbjct: 345 MGDGTGEEVKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVN 404

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           FMV EMG DP+VWEDPMAFKPERFM+  GG EEE A FDITGSK IKMMPFGAGRRMCPG
Sbjct: 405 FMVAEMGWDPEVWEDPMAFKPERFMK--GGEEEEVAGFDITGSKEIKMMPFGAGRRMCPG 464

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           FGLAILHLEYF+ANLVWR EWKAV+GD VDLSEKVE T+VM KPLK N+HPR
Sbjct: 465 FGLAILHLEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMDKPLKANIHPR 514

[Close]

BLAST of Moc02g11670 vs. NCBI nr
Match: KAG6571192.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.1 bits (749), Expect = 1.6e-75
Identity = 141/172 (81.98%), Postives = 152/172 (88.37%), Query Frame = 0


Query: 1   MGKGE-EEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG G  EEVKEE+L KLPYL+AVVLEGLRRHPP HF+LPHAVKEDT L NYVIPKNG+VN
Sbjct: 165 MGDGTGEEVKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVN 224

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           FMV EMG DP+VWEDPMAFKPERFM+  GG EEE A FDITGSK IKMMPFGAGRRMCPG
Sbjct: 225 FMVAEMGWDPEVWEDPMAFKPERFMK--GGEEEEVAGFDITGSKEIKMMPFGAGRRMCPG 284

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           FGLAILHLEYF+ANLVWR +WKAV+GD VDLSEKVE T+VM KPLK N+HPR
Sbjct: 285 FGLAILHLEYFIANLVWRFDWKAVEGDGVDLSEKVEFTVVMDKPLKANIHPR 334

[Close]

BLAST of Moc02g11670 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 243.4 bits (620), Expect = 1.8e-63
Identity = 116/171 (67.84%), Postives = 139/171 (81.29%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           +G+  +EV+EE++EK+PYLKAVVLEGLRRHPP HFLLPH+V EDT LG Y +PKNGT+NF
Sbjct: 340 VGEEAKEVEEEDVEKMPYLKAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINF 399

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV E+G DP  WE+PMAFKPERFM     GEEE  A D+TGS+GIKMMPFGAGRR+CPG 
Sbjct: 400 MVAEIGRDPVEWEEPMAFKPERFM-----GEEE--AVDLTGSRGIKMMPFGAGRRICPGI 459

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           GLA+LHLEY+VAN+V   +WK V G +VDL+EK+E T+VMK PLK    PR
Sbjct: 460 GLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 503

[Close]

BLAST of Moc02g11670 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.1e-60
Identity = 106/167 (63.47%), Postives = 134/167 (80.24%), Query Frame = 0


Query: 6   EEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNFMVGEM 65
           EE++EE+L KL YLKAV+LE LRRHPP H+L  H V  DT LG ++IP+ GT+NFMVGEM
Sbjct: 348 EEIREEDLGKLSYLKAVILECLRRHPPGHYLSYHKVTHDTVLGGFLIPRQGTINFMVGEM 407

Query: 66  GLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGFGLAIL 125
           G DPK+WEDP+ FKPERF+  G     E   FD+TG++ IKMMPFGAGRRMCPG+ L++L
Sbjct: 408 GRDPKIWEDPLTFKPERFLENG-----EACDFDMTGTREIKMMPFGAGRRMCPGYALSLL 467

Query: 126 HLEYFVANLVWRLEWKAVDGDDVDLSEKVE-LTIVMKKPLKVNVHPR 172
           HLEY+VANLVW+ EWK V+G++VDLSEK + +T+VMK P K N++PR
Sbjct: 468 HLEYYVANLVWKFEWKCVEGEEVDLSEKQQFITMVMKNPFKANIYPR 509

[Close]

BLAST of Moc02g11670 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.1e-39
Identity = 81/169 (47.93%), Postives = 107/169 (63.31%), Query Frame = 0


Query: 4   GEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNFMVG 63
           G+++V E ++EK+PYL AVV E LR+HPP +F L H+V E   L  Y IP +  V F V 
Sbjct: 337 GDKKVDENDIEKMPYLNAVVKELLRKHPPTYFTLTHSVTEPVKLAGYDIPMDTNVEFFVH 396

Query: 64  EMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGFGLA 123
            +  DP VW DP  F P+RF+     G E+    DITG K +KMMPFG GRR+CPG G+A
Sbjct: 397 GISHDPNVWSDPEKFDPDRFL----SGREDA---DITGVKEVKMMPFGVGRRICPGLGMA 456

Query: 124 ILHLEYFVANLVWRLEWKAVDGDD-VDLSEKVELTIVMKKPLKVNVHPR 172
            +H+   +A +V   EW A  G++ VD SEK+E T+VMK PL+  V  R
Sbjct: 457 TVHVNLMLARMVQEFEWFAYPGNNKVDFSEKLEFTVVMKNPLRAKVKLR 498

[Close]

BLAST of Moc02g11670 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.0e-37
Identity = 80/169 (47.34%), Postives = 106/169 (62.72%), Query Frame = 0


Query: 4   GEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNFMVG 63
           GE++V E+++EK+PYL AVV E LR+HPP HF+L HAV E T LG Y IP +  V     
Sbjct: 345 GEKKVDEKDVEKMPYLHAVVKELLRKHPPTHFVLTHAVTEPTTLGGYDIPIDANVEVYTP 404

Query: 64  EMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGFGLA 123
            +  DPK W +P  F PERF+    GGEE     DITG  G+KMMPFG GRR+CPG  +A
Sbjct: 405 AIAEDPKNWLNPEKFDPERFI---SGGEEA----DITGVTGVKMMPFGVGRRICPGLAMA 464

Query: 124 ILHLEYFVANLVWRLEWKAVDGD-DVDLSEKVELTIVMKKPLKVNVHPR 172
            +H+   +A +V   EW A   +  +D + K E T+VMK+ L+  + PR
Sbjct: 465 TVHIHLMMARMVQEFEWGAYPPEKKMDFTGKWEFTVVMKESLRATIKPR 506

[Close]

BLAST of Moc02g11670 vs. ExPASy Swiss-Prot
Match: P37124 (Cytochrome P450 77A2 OS=Solanum melongena OX=4111 GN=CYP77A2 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 9.6e-36
Identity = 73/168 (43.45%), Postives = 101/168 (60.12%), Query Frame = 0


Query: 4   GEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNFMVG 63
           GE ++ E+++EK+PYL AVV E LR+HPP +  L HAV E   LG Y IP    V   + 
Sbjct: 350 GENKIDEKDIEKMPYLNAVVKELLRKHPPTYMSLTHAVTEPAKLGGYDIPTGVNVEIFLP 409

Query: 64  EMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGFGLA 123
            +  DP +W +P  F P+RF      G+E+    DITG  G+KM+PFG GRR+CPG  +A
Sbjct: 410 GISDDPNLWSEPEKFDPDRFYL----GKEDA---DITGVSGVKMIPFGMGRRICPGLNMA 469

Query: 124 ILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
            +H+   +A LV   EW   +   VD +EK+E T+VMK  L+  + PR
Sbjct: 470 TVHVSLMLARLVQEFEWADPENTRVDFTEKLEFTVVMKNTLRAKIKPR 510

[Close]

BLAST of Moc02g11670 vs. ExPASy TrEMBL
Match: A0A6J1D4C6 (cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017160 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.6e-78
Identity = 142/171 (83.04%), Postives = 152/171 (88.89%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           MGKGEEEVKEE+L KLPYLKAVVLEGLRRHPP HF+LPHAVKEDT L NY IPKNGTVNF
Sbjct: 348 MGKGEEEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKEDTTLENYAIPKNGTVNF 407

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV +MG DPKVWEDPMAFKPERFM  GGGGE ET AFDITGSK IKMMPFGAGRRMCPG 
Sbjct: 408 MVAQMGWDPKVWEDPMAFKPERFMNGGGGGEGETIAFDITGSKEIKMMPFGAGRRMCPGL 467

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           G+A+LHLEYF+ANLVWR EWKAV GDDVDLSEKVE T+VM+KPLK  ++ R
Sbjct: 468 GVAMLHLEYFIANLVWRFEWKAVAGDDVDLSEKVEFTVVMEKPLKAALNSR 518

[Close]

BLAST of Moc02g11670 vs. ExPASy TrEMBL
Match: A0A0A0LTQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435540 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.2e-77
Identity = 145/172 (84.30%), Postives = 153/172 (88.95%), Query Frame = 0


Query: 1   MGKG-EEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG G EEEVKEE+L KLPYLKAV+LEGLRRHPPAHFLLPHAVKE+T LGNYVIPKNGT N
Sbjct: 342 MGDGSEEEVKEEDLGKLPYLKAVILEGLRRHPPAHFLLPHAVKEETKLGNYVIPKNGTTN 401

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           +MV EMG DPKVWEDPMAFKPERFM+    G EE   FDITGSK IKMMPFGAGRRMCPG
Sbjct: 402 YMVAEMGRDPKVWEDPMAFKPERFMK----GGEEGIEFDITGSKEIKMMPFGAGRRMCPG 461

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           FGLAILHLEYFVANLVWR EWKAVDGD+VDLSEKVELTIVMKKPL+ N+ PR
Sbjct: 462 FGLAILHLEYFVANLVWRFEWKAVDGDEVDLSEKVELTIVMKKPLQANLRPR 509

[Close]

BLAST of Moc02g11670 vs. ExPASy TrEMBL
Match: A0A6J1FY77 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448402 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.4e-76
Identity = 142/172 (82.56%), Postives = 152/172 (88.37%), Query Frame = 0


Query: 1   MGKGE-EEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG G  EEVKEE+L KLPYL+AVVLEGLRRHPP HF+LPHAVKEDT L NYVIPKNG+VN
Sbjct: 345 MGDGTGEEVKEEDLGKLPYLRAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVN 404

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           FMV EMG DP+VWEDPMAFKPERFM+  GG EEE A FDITGSK IKMMPFGAGRRMCPG
Sbjct: 405 FMVAEMGWDPEVWEDPMAFKPERFMK--GGEEEEVAGFDITGSKEIKMMPFGAGRRMCPG 464

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           FGLAILHLEYF+ANLVWR EWKAV+GD VDLSEKVE T+VM KPLK N+HPR
Sbjct: 465 FGLAILHLEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMDKPLKANIHPR 514

[Close]

BLAST of Moc02g11670 vs. ExPASy TrEMBL
Match: A0A6J1D4A2 (cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017161 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.8e-76
Identity = 139/171 (81.29%), Postives = 153/171 (89.47%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           M  G EEVKEE+L KLPYLKAVVLE LRRHPPAHFLLPHAVKEDT   NY+IPKNGTVNF
Sbjct: 343 MENGGEEVKEEDLGKLPYLKAVVLEALRRHPPAHFLLPHAVKEDTVFENYLIPKNGTVNF 402

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV EMGLDPKVWEDPMAFKPERF+++   GEEETAAFDITG+K IKMMPFGAGRR+CPGF
Sbjct: 403 MVAEMGLDPKVWEDPMAFKPERFVKV--AGEEETAAFDITGNKEIKMMPFGAGRRICPGF 462

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           GLAILHLEYFVANL+WR EW AV+GD VDLSEK+E T+VM+KPLK N++PR
Sbjct: 463 GLAILHLEYFVANLIWRFEWTAVEGDGVDLSEKLEFTVVMEKPLKANINPR 511

[Close]

BLAST of Moc02g11670 vs. ExPASy TrEMBL
Match: A0A6J1FYJ0 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448726 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.8e-76
Identity = 142/173 (82.08%), Postives = 153/173 (88.44%), Query Frame = 0


Query: 1   MGKGE-EEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVN 60
           MG G  EEVKEE+L KLPYLKAVVLEGLRRHPP HF+LPHAVK+DT L NYVIPKNGTVN
Sbjct: 350 MGDGTGEEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLPHAVKDDTMLENYVIPKNGTVN 409

Query: 61  FMVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPG 120
           FMV EMG DP+VWEDPMAFKPERFM+  GG EEE A FDITGSK IKMMPFGAGRRMCPG
Sbjct: 410 FMVAEMGWDPEVWEDPMAFKPERFMK--GGEEEEVAGFDITGSKEIKMMPFGAGRRMCPG 469

Query: 121 FGLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPRN 173
           FGLAILHLEYF+ANLVWR EWKAV+GD VDLSEKVE T+VM+KPLK N+ PR+
Sbjct: 470 FGLAILHLEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVMEKPLKANIIPRS 520

[Close]

BLAST of Moc02g11670 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 243.4 bits (620), Expect = 1.3e-64
Identity = 116/171 (67.84%), Postives = 139/171 (81.29%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           +G+  +EV+EE++EK+PYLKAVVLEGLRRHPP HFLLPH+V EDT LG Y +PKNGT+NF
Sbjct: 340 VGEEAKEVEEEDVEKMPYLKAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINF 399

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV E+G DP  WE+PMAFKPERFM     GEEE  A D+TGS+GIKMMPFGAGRR+CPG 
Sbjct: 400 MVAEIGRDPVEWEEPMAFKPERFM-----GEEE--AVDLTGSRGIKMMPFGAGRRICPGI 459

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           GLA+LHLEY+VAN+V   +WK V G +VDL+EK+E T+VMK PLK    PR
Sbjct: 460 GLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 503

[Close]

BLAST of Moc02g11670 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 238.8 bits (608), Expect = 3.2e-63
Identity = 111/171 (64.91%), Postives = 135/171 (78.95%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           +G+   EV+EE+ +K+PYL+AVV+EGLRRHPP HF+LPH+V EDT LG Y +PKNGT+NF
Sbjct: 346 VGEEANEVEEEDAQKMPYLEAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKNGTINF 405

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV E+G DPKVWE+PMAFKPERFM           A DITGS+GIKMMPFGAGRR+CPG 
Sbjct: 406 MVAEIGRDPKVWEEPMAFKPERFME---------EAVDITGSRGIKMMPFGAGRRICPGI 465

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           GLA+LHLEY+VAN+V   +WK V G +VDL+EK+E T+VMK PLK    PR
Sbjct: 466 GLAMLHLEYYVANMVREFDWKEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 507

[Close]

BLAST of Moc02g11670 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 236.9 bits (603), Expect = 1.2e-62
Identity = 110/171 (64.33%), Postives = 135/171 (78.95%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           +G+  +EV+E++ +K+PYLKAVV+EGLRRHPP HF+LPH+V EDT LG Y +PK GT+NF
Sbjct: 347 VGEEAKEVEEQDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINF 406

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV E+G DPKVWE+PMAFKPERFM           A DITGS+GIKMMPFGAGRR+CPG 
Sbjct: 407 MVAEIGRDPKVWEEPMAFKPERFME---------EAVDITGSRGIKMMPFGAGRRICPGI 466

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLKVNVHPR 172
           GLA+LHLEY+VAN+V   EW+ V G +VDL+EK+E T+VMK PLK    PR
Sbjct: 467 GLAMLHLEYYVANMVREFEWQEVQGHEVDLTEKLEFTVVMKHPLKALAVPR 508

[Close]

BLAST of Moc02g11670 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 2.7e-62
Identity = 112/165 (67.88%), Postives = 134/165 (81.21%), Query Frame = 0


Query: 1   MGKGEEEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNF 60
           +G+  +EV+EE+ +K+PYLKAVV+EGLRRHPP HF+LPH+V EDT LG Y +PK GT+NF
Sbjct: 346 VGEEAKEVEEEDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINF 405

Query: 61  MVGEMGLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGF 120
           MV E+G DP VWE+PMAFKPERFM     GEEE  A DITGS+GIKMMPFGAGRR+CPG 
Sbjct: 406 MVAEIGRDPMVWEEPMAFKPERFM-----GEEE--AVDITGSRGIKMMPFGAGRRICPGI 465

Query: 121 GLAILHLEYFVANLVWRLEWKAVDGDDVDLSEKVELTIVMKKPLK 166
           GLA+LHLEY+VAN+V   EWK V G +VDL+EK E T+VMK  LK
Sbjct: 466 GLAMLHLEYYVANMVREFEWKEVQGHEVDLTEKFEFTVVMKHSLK 503

[Close]

BLAST of Moc02g11670 vs. TAIR 10
Match: AT3G03470.1 (cytochrome P450, family 87, subfamily A, polypeptide 9 )

HSP 1 Score: 234.2 bits (596), Expect = 8.0e-62
Identity = 106/167 (63.47%), Postives = 134/167 (80.24%), Query Frame = 0


Query: 6   EEVKEEELEKLPYLKAVVLEGLRRHPPAHFLLPHAVKEDTNLGNYVIPKNGTVNFMVGEM 65
           EE++EE+L KL YLKAV+LE LRRHPP H+L  H V  DT LG ++IP+ GT+NFMVGEM
Sbjct: 348 EEIREEDLGKLSYLKAVILECLRRHPPGHYLSYHKVTHDTVLGGFLIPRQGTINFMVGEM 407

Query: 66  GLDPKVWEDPMAFKPERFMRMGGGGEEETAAFDITGSKGIKMMPFGAGRRMCPGFGLAIL 125
           G DPK+WEDP+ FKPERF+  G     E   FD+TG++ IKMMPFGAGRRMCPG+ L++L
Sbjct: 408 GRDPKIWEDPLTFKPERFLENG-----EACDFDMTGTREIKMMPFGAGRRMCPGYALSLL 467

Query: 126 HLEYFVANLVWRLEWKAVDGDDVDLSEKVE-LTIVMKKPLKVNVHPR 172
           HLEY+VANLVW+ EWK V+G++VDLSEK + +T+VMK P K N++PR
Sbjct: 468 HLEYYVANLVWKFEWKCVEGEEVDLSEKQQFITMVMKNPFKANIYPR 509

The following BLAST results are available for this feature:

BLAST of Moc02g11670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902538.1	5.7e-78	84.30	cytochrome P450 89A2-like [Benincasa hispida]	[more]
XP_022148533.1	7.4e-78	83.04	cytochrome P450 89A2-like [Momordica charantia]	[more]
XP_011650074.1	1.1e-76	84.30	cytochrome P450 89A2 [Cucumis sativus] >KGN63391.1 hypothetical protein Csa_0224...	[more]
XP_022943745.1	7.0e-76	82.56	cytochrome P450 89A2-like [Cucurbita moschata]	[more]
KAG6571192.1	1.6e-75	81.98	Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Moc02g11670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42602	1.8e-63	67.84	Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2	[more]
Q9SRQ1	1.1e-60	63.47	Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1	[more]
P37123	7.1e-39	47.93	Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=...	[more]
O48928	3.0e-37	47.34	Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1	[more]
P37124	9.6e-36	43.45	Cytochrome P450 77A2 OS=Solanum melongena OX=4111 GN=CYP77A2 PE=2 SV=1	[more]

BLAST of Moc02g11670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4C6	3.6e-78	83.04	cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017160 PE=3 SV...	[more]
A0A0A0LTQ9	5.2e-77	84.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435540 PE=3 SV=1	[more]
A0A6J1FY77	3.4e-76	82.56	cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448402 PE=3 SV=...	[more]
A0A6J1D4A2	9.8e-76	81.29	cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017161 PE=3 SV...	[more]
A0A6J1FYJ0	9.8e-76	82.08	cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448726 PE=3 SV=...	[more]

BLAST of Moc02g11670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64900.1	1.3e-64	67.84	cytochrome P450, family 89, subfamily A, polypeptide 2	[more]
AT1G64950.1	3.2e-63	64.91	cytochrome P450, family 89, subfamily A, polypeptide 5	[more]
AT1G64940.1	1.2e-62	64.33	cytochrome P450, family 87, subfamily A, polypeptide 6	[more]
AT2G12190.1	2.7e-62	67.88	Cytochrome P450 superfamily protein	[more]
AT3G03470.1	8.0e-62	63.47	cytochrome P450, family 87, subfamily A, polypeptide 9	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 16..32 score: 38.91 coord: 66..84 score: 32.79 coord: 101..117 score: 35.97 coord: 117..135 score: 28.54
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..173 e-value: 5.2E-49 score: 169.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 5..171
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 6..165 e-value: 1.0E-39 score: 136.6
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 3..170
None	No IPR available	PANTHER	PTHR24298:SF852	P450, PUTATIVE-RELATED	coord: 3..170
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 110..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g11670.1	Moc02g11670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen