Moc02g11580 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g11580
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncytochrome P450 89A2-like
Locationchr2: 8357649 .. 8359043 (+)
RNA-Seq ExpressionMoc02g11580
SyntenyMoc02g11580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCGCCGCTCCCCTCTCCAAATGGAGTCCCTCCTCCGCGCCGCCGTCGCCAAATACGGCCCCACATCACCCTCCGCGTCGGCTCCCACCCCGCCATCTTCATCGCCGACCGCACAATCGCCCACGCCGCCCTCGTCCTCAACGGCGCTGTCTTCGCCCACCGCCCACCGGCTCTTCCCGTCAGAAAGATCGCCTCCAGCAACCAGCACAACATCAATTCCGCCTCCTACGGCCCTCTCTGGCGCCTCCTCCGCCGCAATCTCACCTCCCAAATCCTCCACCCTTCGCGGGTCGCGTCCTACGGGGAAGCTCGCAATTGGGTTTTGGATATTCTCATCAATCGCCTTCTCTCCCACTCTGAATCGGGATTTTCCCCCGTCTCTGTAATCGATCATTTCCAGTACGCCATGTTCTGTTTGCTGGTGCTGATGTGCTTTGGGGATAAGCTCAAGGAAACCAAAATCAAGGAGATCGAGTACGTGCATCGCGCGATGCTTGTGGATTTCAGGCGTTTTGAGGTCCTCAATTTGTGGCCCAAATTGACCAAGATTTTGCTCCGGAGGCGCTGGGAGGAGTTTTTCCAATTGAAGAGAAATCAAGAGAATGTTCTGATTCCTTTGATCGAAGCCCAGAAAAACAGAGACGCTGAAATTGAAGGAGAAGAGGAATTCGTGGTTTCGTATGTTGATACGCTACTCGATTTGAAGATCCCCGACGAGGAAAATCGAAACTTAACGGATGGCGAAATGGTCACTTTATGTTCCGAATTCCTCACCGCTGGGACGGATACCACCTCCATAGCGTTGCAATGGACAATGGCGAATTTGGTGAAATACCCTGAAATCCAACACAAGCTTTTCGCGGAAATCGAAGGGGTTATGGGAAATGGAGGGGAGGAGGTGAAGGAAGGAGATTTGGGGAAGCTTCCATATCTGAAAGCTGTGGTTTTAGAAGGATTAAGAAGACACCCACCAACGCATTTCGTGCCGCCACACGCAGTGAAAGAAGATACGAATCTGGAGAATTATGTGATTCCGAAGAATGGGACGGTAAATTTCATGGTGGCGGAGATGGGTCTGGATCCACAAGTGTGGGAGGATCCGACGGCGTTCAAGCCGGAGAGGTTCATGGAAGGAGAAGAAACGGCGGCGGCGTTGGCGTTTGATGTAACAGGGAGTAAGGAGATAAAAATGATGCCGTTCGGAGCAGGGAGAAGGATTTGTCCAGCGTATGGTGTGGCGATGCTTCATTTGGAATATTTCGTTGCTAATTTGGTTTGGCGATTTGAGTGGAAGGCCGTCGACGGAGACGACGTTGACCTATCGGAGAAGCAAGAGTTGACCGTCGTGATGGAAAACCCTCTGAAAGCCCACATAAGTTCAAGGTTTTAA

mRNA sequence

ATGGCTCCGCCGCTCCCCTCTCCAAATGGAGTCCCTCCTCCGCGCCGCCGTCGCCAAATACGGCCCCACATCACCCTCCGCGTCGGCTCCCACCCCGCCATCTTCATCGCCGACCGCACAATCGCCCACGCCGCCCTCGTCCTCAACGGCGCTGTCTTCGCCCACCGCCCACCGGCTCTTCCCGTCAGAAAGATCGCCTCCAGCAACCAGCACAACATCAATTCCGCCTCCTACGGCCCTCTCTGGCGCCTCCTCCGCCGCAATCTCACCTCCCAAATCCTCCACCCTTCGCGGGTCGCGTCCTACGGGGAAGCTCGCAATTGGGTTTTGGATATTCTCATCAATCGCCTTCTCTCCCACTCTGAATCGGGATTTTCCCCCGTCTCTGTAATCGATCATTTCCAGTACGCCATGTTCTGTTTGCTGGTGCTGATGTGCTTTGGGGATAAGCTCAAGGAAACCAAAATCAAGGAGATCGAGTACGTGCATCGCGCGATGCTTGTGGATTTCAGGCGTTTTGAGGTCCTCAATTTGTGGCCCAAATTGACCAAGATTTTGCTCCGGAGGCGCTGGGAGGAGTTTTTCCAATTGAAGAGAAATCAAGAGAATGTTCTGATTCCTTTGATCGAAGCCCAGAAAAACAGAGACGCTGAAATTGAAGGAGAAGAGGAATTCGTGGTTTCGTATGTTGATACGCTACTCGATTTGAAGATCCCCGACGAGGAAAATCGAAACTTAACGGATGGCGAAATGGTCACTTTATGTTCCGAATTCCTCACCGCTGGGACGGATACCACCTCCATAGCGTTGCAATGGACAATGGCGAATTTGGTGAAATACCCTGAAATCCAACACAAGCTTTTCGCGGAAATCGAAGGGGTTATGGGAAATGGAGGGGAGGAGGTGAAGGAAGGAGATTTGGGGAAGCTTCCATATCTGAAAGCTGTGGTTTTAGAAGGATTAAGAAGACACCCACCAACGCATTTCGTGCCGCCACACGCAGTGAAAGAAGATACGAATCTGGAGAATTATGTGATTCCGAAGAATGGGACGGTAAATTTCATGGTGGCGGAGATGGGTCTGGATCCACAAGTGTGGGAGGATCCGACGGCGTTCAAGCCGGAGAGGTTCATGGAAGGAGAAGAAACGGCGGCGGCGTTGGCGTTTGATGTAACAGGGAGTAAGGAGATAAAAATGATGCCGTTCGGAGCAGGGAGAAGGATTTGTCCAGCGTATGGTGTGGCGATGCTTCATTTGGAATATTTCGTTGCTAATTTGGTTTGGCGATTTGAGTGGAAGGCCGTCGACGGAGACGACGTTGACCTATCGGAGAAGCAAGAGTTGACCGTCGTGATGGAAAACCCTCTGAAAGCCCACATAAGTTCAAGGTTTTAA

Coding sequence (CDS)

ATGGCTCCGCCGCTCCCCTCTCCAAATGGAGTCCCTCCTCCGCGCCGCCGTCGCCAAATACGGCCCCACATCACCCTCCGCGTCGGCTCCCACCCCGCCATCTTCATCGCCGACCGCACAATCGCCCACGCCGCCCTCGTCCTCAACGGCGCTGTCTTCGCCCACCGCCCACCGGCTCTTCCCGTCAGAAAGATCGCCTCCAGCAACCAGCACAACATCAATTCCGCCTCCTACGGCCCTCTCTGGCGCCTCCTCCGCCGCAATCTCACCTCCCAAATCCTCCACCCTTCGCGGGTCGCGTCCTACGGGGAAGCTCGCAATTGGGTTTTGGATATTCTCATCAATCGCCTTCTCTCCCACTCTGAATCGGGATTTTCCCCCGTCTCTGTAATCGATCATTTCCAGTACGCCATGTTCTGTTTGCTGGTGCTGATGTGCTTTGGGGATAAGCTCAAGGAAACCAAAATCAAGGAGATCGAGTACGTGCATCGCGCGATGCTTGTGGATTTCAGGCGTTTTGAGGTCCTCAATTTGTGGCCCAAATTGACCAAGATTTTGCTCCGGAGGCGCTGGGAGGAGTTTTTCCAATTGAAGAGAAATCAAGAGAATGTTCTGATTCCTTTGATCGAAGCCCAGAAAAACAGAGACGCTGAAATTGAAGGAGAAGAGGAATTCGTGGTTTCGTATGTTGATACGCTACTCGATTTGAAGATCCCCGACGAGGAAAATCGAAACTTAACGGATGGCGAAATGGTCACTTTATGTTCCGAATTCCTCACCGCTGGGACGGATACCACCTCCATAGCGTTGCAATGGACAATGGCGAATTTGGTGAAATACCCTGAAATCCAACACAAGCTTTTCGCGGAAATCGAAGGGGTTATGGGAAATGGAGGGGAGGAGGTGAAGGAAGGAGATTTGGGGAAGCTTCCATATCTGAAAGCTGTGGTTTTAGAAGGATTAAGAAGACACCCACCAACGCATTTCGTGCCGCCACACGCAGTGAAAGAAGATACGAATCTGGAGAATTATGTGATTCCGAAGAATGGGACGGTAAATTTCATGGTGGCGGAGATGGGTCTGGATCCACAAGTGTGGGAGGATCCGACGGCGTTCAAGCCGGAGAGGTTCATGGAAGGAGAAGAAACGGCGGCGGCGTTGGCGTTTGATGTAACAGGGAGTAAGGAGATAAAAATGATGCCGTTCGGAGCAGGGAGAAGGATTTGTCCAGCGTATGGTGTGGCGATGCTTCATTTGGAATATTTCGTTGCTAATTTGGTTTGGCGATTTGAGTGGAAGGCCGTCGACGGAGACGACGTTGACCTATCGGAGAAGCAAGAGTTGACCGTCGTGATGGAAAACCCTCTGAAAGCCCACATAAGTTCAAGGTTTTAA

Protein sequence

MAPPLPSPNGVPPPRRRRQIRPHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPLWRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCLLVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQENVLIPLIEAQKNRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTLCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLKAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKPERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWKAVDGDDVDLSEKQELTVVMENPLKAHISSRF
Homology
BLAST of Moc02g11580 vs. NCBI nr
Match: XP_022148526.1 (cytochrome P450 89A2-like [Momordica charantia])

HSP 1 Score: 715.7 bits (1846), Expect = 2.6e-202
Identity = 360/450 (80.00%), Postives = 391/450 (86.89%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+ + PAI+IADR+IAH ALV +GA+FA RPPA P+ KIASSNQH+INSASYGPL
Sbjct: 66  PIVTLRIAARPAIYIADRSIAHKALVRDGALFADRPPAPPITKIASSNQHSINSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLTSQILHPSRV SYG AR WVLDILINRLLSHSESG +PV+V+ HFQYAMFCL
Sbjct: 126 WRLLRRNLTSQILHPSRVRSYGRARKWVLDILINRLLSHSESG-TPVNVVQHFQYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL ET+I+E+E   R+ML+   RF VLNL P L+KI LR+RWEEFF+L+RNQ
Sbjct: 186 LVLMCFGDKLDETQIREVEKAIRSMLLRVGRFGVLNLCPTLSKIFLRKRWEEFFELRRNQ 245

Query: 202 ENVLIPLIE----AQKNRDAEIE---GEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E VLIPLIE    AQKNR A+IE   GEEEFV+SYVDTLLDLK+P+EENR+LT+ EMVTL
Sbjct: 246 EKVLIPLIEARRKAQKNRAAQIEGGGGEEEFVLSYVDTLLDLKLPEEENRSLTNSEMVTL 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLK 321
           C+EFL AGTDTTS ALQW MANLVKYPEIQ KLFAEIEGVMG G EEVKE DLGK PYLK
Sbjct: 306 CAEFLNAGTDTTSTALQWIMANLVKYPEIQRKLFAEIEGVMGKGEEEVKEEDLGKFPYLK 365

Query: 322 AVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKP 381
           AVVLEGLRRHPP HFV PHAVKEDTNLENYVIPKNG VNFMV EMGLDPQVWEDP AFKP
Sbjct: 366 AVVLEGLRRHPPGHFVLPHAVKEDTNLENYVIPKNGMVNFMVGEMGLDPQVWEDPLAFKP 425

Query: 382 ERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWKA 441
           ERFME EET    AFD+TGSKEIKMMPFG GRRICP  GVAMLHLEYF+ANLVWRFEWKA
Sbjct: 426 ERFME-EETGTVAAFDITGSKEIKMMPFGTGRRICPGLGVAMLHLEYFIANLVWRFEWKA 485

Query: 442 VDGDDVDLSEKQELTVVMENPLKAHISSRF 465
           V GDDVDLSEK E TVVME PLKAH+SSRF
Sbjct: 486 VAGDDVDLSEKIEFTVVMEKPLKAHVSSRF 513

BLAST of Moc02g11580 vs. NCBI nr
Match: XP_022986504.1 (cytochrome P450 89A2-like [Cucurbita maxima])

HSP 1 Score: 696.0 bits (1795), Expect = 2.1e-196
Identity = 340/450 (75.56%), Postives = 388/450 (86.22%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+G+ P IFI+DR+IAH AL+ NGA+FA RPPA P+ KI SSNQHNI+SASYGPL
Sbjct: 66  PVVTLRIGNRPTIFISDRSIAHKALLQNGALFADRPPAPPMSKIISSNQHNISSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLT QILHPSRV SY  AR WVLDIL+NRL+SHS+S  +PV+VIDHF YAMFCL
Sbjct: 126 WRLLRRNLTFQILHPSRVKSYNRARKWVLDILLNRLVSHSKSE-TPVTVIDHFHYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL+E +I EIE VHRAML+ FRRF VLN WPKLTKILLR+RWE FF+++RNQ
Sbjct: 186 LVLMCFGDKLEEPQIHEIENVHRAMLLSFRRFNVLNFWPKLTKILLRKRWEAFFEIRRNQ 245

Query: 202 ENVLIPLIEAQK-------NRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E V+IPLIEA++       NR++  E EE+FVVSYVDTLLDL++PDE+NR LTD E+VT+
Sbjct: 246 EKVIIPLIEARRKANQNRVNRESRAETEEDFVVSYVDTLLDLELPDEDNRKLTDEEIVTI 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNG-GEEVKEGDLGKLPYL 321
           CSEFL AGTDTTS ALQW MANLVKYPEIQHKLFAE++GVMG+G GEEVKE DLGKLPYL
Sbjct: 306 CSEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVMGDGTGEEVKEEDLGKLPYL 365

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           +AVVLEGLRRHPP HFV PHAVKEDT LENYVIPKNGTVNFMVAEMG DP+VWE+P AFK
Sbjct: 366 RAVVLEGLRRHPPGHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEMGWDPEVWEEPMAFK 425

Query: 382 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 441
           PERFM+G E      FD+TGSKEIKMMPFGAGRR+CP +G+A+LHLEYF+ANLVWRFEWK
Sbjct: 426 PERFMKGGEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRFEWK 485

Query: 442 AVDGDDVDLSEKQELTVVMENPLKAHISSR 464
            V+GD VDLSEK E TVVM+ PLKA+I  R
Sbjct: 486 VVEGDGVDLSEKAEFTVVMDKPLKANIHPR 514

BLAST of Moc02g11580 vs. NCBI nr
Match: XP_022943745.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 689.9 bits (1779), Expect = 1.5e-194
Identity = 338/450 (75.11%), Postives = 385/450 (85.56%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+ + P IFI+DR+IAH AL+ NGA+FA RPPA P+ KI SSNQHNI+SASYGPL
Sbjct: 66  PVVTLRIANRPTIFISDRSIAHKALLQNGALFADRPPAPPMSKIISSNQHNISSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLT QILHPSRV SY  AR WVLDIL+NRL+SHS+S  +PV+VIDHF YAMFCL
Sbjct: 126 WRLLRRNLTFQILHPSRVKSYNRARKWVLDILLNRLVSHSKSE-TPVTVIDHFHYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL+E +I EIE VHRAML+ FRRF VLN WPKLTKILLR+RWE F +++RNQ
Sbjct: 186 LVLMCFGDKLEEPQIHEIENVHRAMLLSFRRFNVLNFWPKLTKILLRKRWEAFLEIRRNQ 245

Query: 202 ENVLIPLIEAQK-------NRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E V+IPLIEA++       NR+   E EE FVVSYVDTLLDL++PDE+NR LTD E+VT+
Sbjct: 246 EKVIIPLIEARRKANQNRANRENRAETEEAFVVSYVDTLLDLELPDEDNRKLTDEEIVTI 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNG-GEEVKEGDLGKLPYL 321
           CSEFL AGTDTTS ALQW MAN+VKYPEIQHKLFAE++GVMG+G GEEVKE DLGKLPYL
Sbjct: 306 CSEFLNAGTDTTSTALQWIMANIVKYPEIQHKLFAEMKGVMGDGTGEEVKEEDLGKLPYL 365

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           +AVVLEGLRRHPP HFV PHAVKEDT LENYVIPKNG+VNFMVAEMG DP+VWEDP AFK
Sbjct: 366 RAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVNFMVAEMGWDPEVWEDPMAFK 425

Query: 382 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 441
           PERFM+G E      FD+TGSKEIKMMPFGAGRR+CP +G+A+LHLEYF+ANLVWRFEWK
Sbjct: 426 PERFMKGGEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRFEWK 485

Query: 442 AVDGDDVDLSEKQELTVVMENPLKAHISSR 464
           AV+GD VDLSEK E TVVM+ PLKA+I  R
Sbjct: 486 AVEGDGVDLSEKVEFTVVMDKPLKANIHPR 514

BLAST of Moc02g11580 vs. NCBI nr
Match: KAG7010999.1 (Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 687.6 bits (1773), Expect = 7.6e-194
Identity = 338/450 (75.11%), Postives = 387/450 (86.00%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+ + P IFI+DR+IAH AL+ NGA+FA RPPA P+ KI SSNQHNI+SASYGPL
Sbjct: 143 PVVTLRIANRPTIFISDRSIAHKALLQNGALFADRPPAPPMSKIISSNQHNISSASYGPL 202

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLT QILHPSRV SY  AR WVLDIL+NRL+SHS+S  +PV+VIDHF YAMFCL
Sbjct: 203 WRLLRRNLTFQILHPSRVKSYNRARKWVLDILLNRLVSHSKSE-TPVTVIDHFHYAMFCL 262

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL+E +I EIE VHRAML+ FRRF VLN WPKLTKILLR+RWE FF+++RNQ
Sbjct: 263 LVLMCFGDKLEEPQIHEIENVHRAMLLSFRRFNVLNFWPKLTKILLRKRWEAFFEIRRNQ 322

Query: 202 ENVLIPLIEAQK----------NRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEM 261
           E V+IPLIEA++          NR+   E EE+FVVSYVDTLLDL++PDE+NR LTD E+
Sbjct: 323 EKVIIPLIEARRKANQNRANRENRENRAETEEDFVVSYVDTLLDLELPDEDNRKLTDEEI 382

Query: 262 VTLCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNG-GEEVKEGDLGKL 321
           VT+CSEFL AGTDTTS ALQW MAN+VKYPEIQHKLFAE++GVMG+G GEEVKE DLGKL
Sbjct: 383 VTICSEFLNAGTDTTSTALQWIMANIVKYPEIQHKLFAEMKGVMGDGKGEEVKEEDLGKL 442

Query: 322 PYLKAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPT 381
           PYL+AVVLEGLRRHPP HFV PHAVKEDT LENYVIPKNG+VNFMVAEMG DP+VWEDP 
Sbjct: 443 PYLRAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVNFMVAEMGWDPEVWEDPM 502

Query: 382 AFKPERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRF 441
           AFKPERFM+G E   A  FD+TGSKEIKMMPFGAGRR+CP +G+A+LHLEYF+ANLVWRF
Sbjct: 503 AFKPERFMKGGEEEVA-GFDITGSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRF 562

Query: 442 EWKAVDGDDVDLSEKQELTVVMENPLKAHI 461
           +WKAV+GD VDLSEK E TVVM+ PLKA+I
Sbjct: 563 DWKAVEGDGVDLSEKVEFTVVMDKPLKANI 590

BLAST of Moc02g11580 vs. NCBI nr
Match: XP_022148533.1 (cytochrome P450 89A2-like [Momordica charantia])

HSP 1 Score: 686.0 bits (1769), Expect = 2.2e-193
Identity = 343/452 (75.88%), Postives = 383/452 (84.73%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+GS P++FIADR IAHAAL+ + ++FA RPPALPV  + SSNQHNI+SASYGPL
Sbjct: 69  PVVTLRIGSRPSVFIADRHIAHAALIQSASLFADRPPALPVSSVVSSNQHNISSASYGPL 128

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLTSQILHPSRVASYG AR WVLDIL+NR LSH ESG +PV V+ HFQYAMFCL
Sbjct: 129 WRLLRRNLTSQILHPSRVASYGGARKWVLDILLNRFLSHPESG-TPVCVVHHFQYAMFCL 188

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFG++L+ETKI+EIE V+RA+L+   RF +L+ +PKLTK+ LR  WE F QL+RNQ
Sbjct: 189 LVLMCFGNRLEETKIREIENVNRALLLQLGRFRILDFFPKLTKLFLRNSWEAFLQLRRNQ 248

Query: 202 ENVLIPLIEAQKN------RDAEIEGEEE-FVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           ENVLIPLIEA++         A+IE EE+ FVVSYVDTLLDLK+ DE NR+LTDGEMV+L
Sbjct: 249 ENVLIPLIEARRKAKQNRANRAQIEKEEDKFVVSYVDTLLDLKLTDEGNRDLTDGEMVSL 308

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLK 321
           CSEFL AGTDTTS ALQW MANLVKYPEIQ KLFAEIEGVMG G EEVKE DLGKLPYLK
Sbjct: 309 CSEFLNAGTDTTSTALQWIMANLVKYPEIQRKLFAEIEGVMGKGEEEVKEEDLGKLPYLK 368

Query: 322 AVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKP 381
           AVVLEGLRRHPP HFV PHAVKEDT LENY IPKNGTVNFMVA+MG DP+VWEDP AFKP
Sbjct: 369 AVVLEGLRRHPPGHFVLPHAVKEDTTLENYAIPKNGTVNFMVAQMGWDPKVWEDPMAFKP 428

Query: 382 ERFME--GEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEW 441
           ERFM   G      +AFD+TGSKEIKMMPFGAGRR+CP  GVAMLHLEYF+ANLVWRFEW
Sbjct: 429 ERFMNGGGGGEGETIAFDITGSKEIKMMPFGAGRRMCPGLGVAMLHLEYFIANLVWRFEW 488

Query: 442 KAVDGDDVDLSEKQELTVVMENPLKAHISSRF 465
           KAV GDDVDLSEK E TVVME PLKA ++SRF
Sbjct: 489 KAVAGDDVDLSEKVEFTVVMEKPLKAALNSRF 519

BLAST of Moc02g11580 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 548.1 bits (1411), Expect = 9.4e-155
Identity = 268/445 (60.22%), Postives = 341/445 (76.63%), Query Frame = 0

Query: 19  QIRPHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASY 78
           ++ P +TLR+ S PAIF+ADR++ H ALVLNGAV+A RPP   + KI   ++HNI+S SY
Sbjct: 64  RLGPIVTLRITSRPAIFVADRSLTHEALVLNGAVYADRPPPAVISKIV--DEHNISSGSY 123

Query: 79  GPLWRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAM 138
           G  WRLLRRN+TS+ILHPSRV SY  AR+WVL+IL  R  +H   G  P+ +I H  YAM
Sbjct: 124 GATWRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNH--GGEEPIVLIHHLHYAM 183

Query: 139 FCLLVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLK 198
           F LLVLMCFGDKL E +IKE+E++ R  L+   +F + N+WPK TK++LR+RW+EF Q++
Sbjct: 184 FALLVLMCFGDKLDEKQIKEVEFIQRLQLLSLTKFNIFNIWPKFTKLILRKRWQEFLQIR 243

Query: 199 RNQENVLIPLIEA-----QKNRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVT 258
           R Q +VL+PLI A     ++ + +E E ++++V SYVDTLLDL++P EENR L + +++ 
Sbjct: 244 RQQRDVLLPLIRARRKIVEERKRSEQEDKKDYVQSYVDTLLDLELP-EENRKLNEEDIMN 303

Query: 259 LCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYL 318
           LCSEFLTAGTDTT+ ALQW MANLVKYPEIQ +L  EI+ V+G   +EV+E D+ K+PYL
Sbjct: 304 LCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVVGEEAKEVEEEDVEKMPYL 363

Query: 319 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 378
           KAVVLEGLRRHPP HF+ PH+V EDT L  Y +PKNGT+NFMVAE+G DP  WE+P AFK
Sbjct: 364 KAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFK 423

Query: 379 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 438
           PERFM  EE     A D+TGS+ IKMMPFGAGRRICP  G+AMLHLEY+VAN+V  F+WK
Sbjct: 424 PERFMGEEE-----AVDLTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWK 483

Query: 439 AVDGDDVDLSEKQELTVVMENPLKA 459
            V G +VDL+EK E TVVM++PLKA
Sbjct: 484 EVQGHEVDLTEKLEFTVVMKHPLKA 498

BLAST of Moc02g11580 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.4e-137
Identity = 243/451 (53.88%), Postives = 330/451 (73.17%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P ITL VGS P+I++ DR++AH ALV NGAVF+ R  ALP  K+ +SNQH+I+S+ YG L
Sbjct: 68  PIITLHVGSKPSIWVTDRSLAHQALVQNGAVFSDRSLALPTTKVITSNQHDIHSSVYGSL 127

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WR LRRNLTS+IL PSRV ++  +R W L+IL++   +           +DH ++AMF L
Sbjct: 128 WRTLRRNLTSEILQPSRVKAHAPSRKWSLEILVDLFETEQREKGHISDALDHLRHAMFYL 187

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           L LMCFG+KL++ +I+EIE     ML+ + +F VLN++P +TK LLRR+W+EF +L+++Q
Sbjct: 188 LALMCFGEKLRKEEIREIEEAQYQMLISYTKFSVLNIFPSVTKFLLRRKWKEFLELRKSQ 247

Query: 202 ENVLIPLIEAQKNRDAEIEGEEEFVVSYVDTLLDLKIPDEE-----NRNLTDGEMVTLCS 261
           E+V++  + A   R  E  G+   V+ YVDTLL+L+IP EE      R L+D E+V+LCS
Sbjct: 248 ESVILRYVNA---RSKETTGD---VLCYVDTLLNLEIPTEEKEGGKKRKLSDSEIVSLCS 307

Query: 262 EFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMG---NGGEEVKEGDLGKLPYL 321
           EFL A TD T+ ++QW MA +VKYPEIQ K++ E++ V        EE++E DLGKL YL
Sbjct: 308 EFLNAATDPTATSMQWIMAIMVKYPEIQRKVYEEMKTVFAGEEEEREEIREEDLGKLSYL 367

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           KAV+LE LRRHPP H++  H V  DT L  ++IP+ GT+NFMV EMG DP++WEDP  FK
Sbjct: 368 KAVILECLRRHPPGHYLSYHKVTHDTVLGGFLIPRQGTINFMVGEMGRDPKIWEDPLTFK 427

Query: 382 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 441
           PERF+E  E   A  FD+TG++EIKMMPFGAGRR+CP Y +++LHLEY+VANLVW+FEWK
Sbjct: 428 PERFLENGE---ACDFDMTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLVWKFEWK 487

Query: 442 AVDGDDVDLSEKQE-LTVVMENPLKAHISSR 464
            V+G++VDLSEKQ+ +T+VM+NP KA+I  R
Sbjct: 488 CVEGEEVDLSEKQQFITMVMKNPFKANIYPR 509

BLAST of Moc02g11580 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.0e-88
Identity = 182/445 (40.90%), Postives = 271/445 (60.90%), Query Frame = 0

Query: 25  TLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPLWRL 84
           TL++GS   I +A   +AH AL+  G +FA RP   P R I S N+ ++N+A YGP+WR 
Sbjct: 66  TLKMGSRTMIIVASAELAHEALIQKGQIFASRPRENPTRTIFSCNKFSVNAAVYGPVWRS 125

Query: 85  LRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCLLVL 144
           LRRN+   +L PSR+  + E R   +D LI R+   ++     V  + + ++A+F +LV 
Sbjct: 126 LRRNMVQNMLSPSRLKEFREFREIAMDKLIERIRVDAKENNDVVWALKNARFAVFYILVA 185

Query: 145 MCFGDKL-KETKIKEIEYVHRAMLV--DFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 204
           MCFG ++  E  I+ ++ + + +L+  D R  + L +         R+R  E   +++ Q
Sbjct: 186 MCFGVEMDNEEMIERVDQMMKDVLIVLDPRIDDFLPILRLFVGYKQRKRVNE---VRKRQ 245

Query: 205 ENVLIPLIEAQKNRDAEIEGEEEFVV--SYVDTLLDLKIPDEENRNLTDGEMVTLCSEFL 264
              L+PLIE +++   +  G ++     SY+DTL D+K+   ++   T+ E+VTLCSEFL
Sbjct: 246 IETLVPLIEKRRS-VVQNPGSDKTAASFSYLDTLFDVKVEGRKS-GPTNAELVTLCSEFL 305

Query: 265 TAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLKAVVLE 324
             GTDTT+ AL+W +  L++ P IQ++L+ EI+ ++G+  ++V E D+ K+PYL AVV E
Sbjct: 306 NGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTIVGD--KKVDENDIEKMPYLNAVVKE 365

Query: 325 GLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKPERFME 384
            LR+HPPT+F   H+V E   L  Y IP +  V F V  +  DP VW DP  F P+RF+ 
Sbjct: 366 LLRKHPPTYFTLTHSVTEPVKLAGYDIPMDTNVEFFVHGISHDPNVWSDPEKFDPDRFLS 425

Query: 385 GEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWKAVDGDD 444
           G E A     D+TG KE+KMMPFG GRRICP  G+A +H+   +A +V  FEW A  G++
Sbjct: 426 GREDA-----DITGVKEVKMMPFGVGRRICPGLGMATVHVNLMLARMVQEFEWFAYPGNN 485

Query: 445 -VDLSEKQELTVVMENPLKAHISSR 464
            VD SEK E TVVM+NPL+A +  R
Sbjct: 486 KVDFSEKLEFTVVMKNPLRAKVKLR 498

BLAST of Moc02g11580 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 7.1e-86
Identity = 174/442 (39.37%), Postives = 264/442 (59.73%), Query Frame = 0

Query: 25  TLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPLWRL 84
           TL++G+   I + D  + H A++  GA +A RPP  P R I S N+  +N+A+YGP+W+ 
Sbjct: 76  TLKMGTRTMIILTDAKLVHEAMIQKGATYATRPPENPTRTIFSENKFTVNAATYGPVWKS 135

Query: 85  LRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCLLVL 144
           LRRN+   +L  +R+  +   R+  +D LINRL   +E     V V+   ++A+FC+LV 
Sbjct: 136 LRRNMVQNMLSSTRLKEFRSVRDNAMDKLINRLKDEAEKNNGVVWVLKDARFAVFCILVA 195

Query: 145 MCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQENV 204
           MCFG ++ E  ++ I+ V +++L+      + +  P L+    ++R ++  +++R Q   
Sbjct: 196 MCFGLEMDEETVERIDQVMKSVLITLDP-RIDDYLPILSPFFSKQR-KKALEVRREQVEF 255

Query: 205 LIPLIEAQKNRDAEIEGEEEFVV--SYVDTLLDLKIPDEENRNLTDGEMVTLCSEFLTAG 264
           L+P+IE Q+ R  +  G +      SY+DTL DLK+  +++   +D E+V+LCSEFL  G
Sbjct: 256 LVPIIE-QRRRAIQNPGSDHTATTFSYLDTLFDLKVEGKKSAP-SDAELVSLCSEFLNGG 315

Query: 265 TDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLKAVVLEGLR 324
           TDTT+ A++W +A L+  P +Q KL+ EI+  +G   ++V E D+ K+PYL AVV E LR
Sbjct: 316 TDTTATAVEWGIAQLIANPNVQTKLYEEIKRTVGE--KKVDEKDVEKMPYLHAVVKELLR 375

Query: 325 RHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKPERFMEGEE 384
           +HPPTHFV  HAV E T L  Y IP +  V      +  DP+ W +P  F PERF+ G E
Sbjct: 376 KHPPTHFVLTHAVTEPTTLGGYDIPIDANVEVYTPAIAEDPKNWLNPEKFDPERFISGGE 435

Query: 385 TAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWKAVDGD-DVD 444
            A     D+TG   +KMMPFG GRRICP   +A +H+   +A +V  FEW A   +  +D
Sbjct: 436 EA-----DITGVTGVKMMPFGVGRRICPGLAMATVHIHLMMARMVQEFEWGAYPPEKKMD 495

Query: 445 LSEKQELTVVMENPLKAHISSR 464
            + K E TVVM+  L+A I  R
Sbjct: 496 FTGKWEFTVVMKESLRATIKPR 506

BLAST of Moc02g11580 vs. ExPASy Swiss-Prot
Match: Q9LZ31 (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.9e-84
Identity = 177/449 (39.42%), Postives = 267/449 (59.47%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P  TLR+G+   I ++D T+ H AL+  GA+FA RP   P R I S N+  +N+A YGP+
Sbjct: 77  PIFTLRMGTRTMIILSDATLVHEALIQRGALFASRPAENPTRTIFSCNKFTVNAAKYGPV 136

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WR LRRN+   +L  +R+  +G+ R   +D LI R+ S +      + V+ + ++A FC+
Sbjct: 137 WRSLRRNMVQNMLSSTRLKEFGKLRQSAMDKLIERIKSEARDNDGLIWVLKNARFAAFCI 196

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAML--VDFRRFEVLNLWPKLTKILLRRRWEEFFQLKR 201
           L+ MCFG ++ E  I++++ + + +L  VD R  + L   P L     + R +   +++R
Sbjct: 197 LLEMCFGIEMDEETIEKMDEILKTVLMTVDPRIDDYL---PILAPFFSKER-KRALEVRR 256

Query: 202 NQENVLIPLIEAQKNRDAEIEGEEEFV--VSYVDTLLDLKIPDEENRNLT--DGEMVTLC 261
            Q + ++ +IE ++ R  +  G ++     SY+DTL DLKI   E R  T  + E+VTLC
Sbjct: 257 EQVDYVVGVIE-RRRRAIQNPGSDKTASSFSYLDTLFDLKI---EGRKTTPSNEELVTLC 316

Query: 262 SEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLKA 321
           SEFL  GTDTT  A++W +A L+  PEIQ +L+ EI+  +G+    V E D+ K+ +L+A
Sbjct: 317 SEFLNGGTDTTGTAIEWGIAQLIANPEIQSRLYDEIKSTVGD-DRRVDEKDVDKMVFLQA 376

Query: 322 VVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKPE 381
            V E LR+HPPT+F   HAV E T L  Y IP    V   +  +  DP++W +P  F P+
Sbjct: 377 FVKELLRKHPPTYFSLTHAVMETTTLAGYDIPAGVNVEVYLPGISEDPRIWNNPKKFDPD 436

Query: 382 RFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWKA- 441
           RFM G+E A     D+TG   +KM+PFG GRRICP   +A +H+   +A +V  FEW A 
Sbjct: 437 RFMLGKEDA-----DITGISGVKMIPFGVGRRICPGLAMATIHVHLMLARMVQEFEWCAH 496

Query: 442 VDGDDVDLSEKQELTVVMENPLKAHISSR 464
             G ++D + K E TVVM+NPL+A +  R
Sbjct: 497 PPGSEIDFAGKLEFTVVMKNPLRAMVKPR 511

BLAST of Moc02g11580 vs. ExPASy TrEMBL
Match: A0A6J1D5N1 (cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017152 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 360/450 (80.00%), Postives = 391/450 (86.89%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+ + PAI+IADR+IAH ALV +GA+FA RPPA P+ KIASSNQH+INSASYGPL
Sbjct: 66  PIVTLRIAARPAIYIADRSIAHKALVRDGALFADRPPAPPITKIASSNQHSINSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLTSQILHPSRV SYG AR WVLDILINRLLSHSESG +PV+V+ HFQYAMFCL
Sbjct: 126 WRLLRRNLTSQILHPSRVRSYGRARKWVLDILINRLLSHSESG-TPVNVVQHFQYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL ET+I+E+E   R+ML+   RF VLNL P L+KI LR+RWEEFF+L+RNQ
Sbjct: 186 LVLMCFGDKLDETQIREVEKAIRSMLLRVGRFGVLNLCPTLSKIFLRKRWEEFFELRRNQ 245

Query: 202 ENVLIPLIE----AQKNRDAEIE---GEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E VLIPLIE    AQKNR A+IE   GEEEFV+SYVDTLLDLK+P+EENR+LT+ EMVTL
Sbjct: 246 EKVLIPLIEARRKAQKNRAAQIEGGGGEEEFVLSYVDTLLDLKLPEEENRSLTNSEMVTL 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLK 321
           C+EFL AGTDTTS ALQW MANLVKYPEIQ KLFAEIEGVMG G EEVKE DLGK PYLK
Sbjct: 306 CAEFLNAGTDTTSTALQWIMANLVKYPEIQRKLFAEIEGVMGKGEEEVKEEDLGKFPYLK 365

Query: 322 AVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKP 381
           AVVLEGLRRHPP HFV PHAVKEDTNLENYVIPKNG VNFMV EMGLDPQVWEDP AFKP
Sbjct: 366 AVVLEGLRRHPPGHFVLPHAVKEDTNLENYVIPKNGMVNFMVGEMGLDPQVWEDPLAFKP 425

Query: 382 ERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWKA 441
           ERFME EET    AFD+TGSKEIKMMPFG GRRICP  GVAMLHLEYF+ANLVWRFEWKA
Sbjct: 426 ERFME-EETGTVAAFDITGSKEIKMMPFGTGRRICPGLGVAMLHLEYFIANLVWRFEWKA 485

Query: 442 VDGDDVDLSEKQELTVVMENPLKAHISSRF 465
           V GDDVDLSEK E TVVME PLKAH+SSRF
Sbjct: 486 VAGDDVDLSEKIEFTVVMEKPLKAHVSSRF 513

BLAST of Moc02g11580 vs. ExPASy TrEMBL
Match: A0A6J1JG80 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111484223 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 1.0e-196
Identity = 340/450 (75.56%), Postives = 388/450 (86.22%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+G+ P IFI+DR+IAH AL+ NGA+FA RPPA P+ KI SSNQHNI+SASYGPL
Sbjct: 66  PVVTLRIGNRPTIFISDRSIAHKALLQNGALFADRPPAPPMSKIISSNQHNISSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLT QILHPSRV SY  AR WVLDIL+NRL+SHS+S  +PV+VIDHF YAMFCL
Sbjct: 126 WRLLRRNLTFQILHPSRVKSYNRARKWVLDILLNRLVSHSKSE-TPVTVIDHFHYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL+E +I EIE VHRAML+ FRRF VLN WPKLTKILLR+RWE FF+++RNQ
Sbjct: 186 LVLMCFGDKLEEPQIHEIENVHRAMLLSFRRFNVLNFWPKLTKILLRKRWEAFFEIRRNQ 245

Query: 202 ENVLIPLIEAQK-------NRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E V+IPLIEA++       NR++  E EE+FVVSYVDTLLDL++PDE+NR LTD E+VT+
Sbjct: 246 EKVIIPLIEARRKANQNRVNRESRAETEEDFVVSYVDTLLDLELPDEDNRKLTDEEIVTI 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNG-GEEVKEGDLGKLPYL 321
           CSEFL AGTDTTS ALQW MANLVKYPEIQHKLFAE++GVMG+G GEEVKE DLGKLPYL
Sbjct: 306 CSEFLNAGTDTTSTALQWIMANLVKYPEIQHKLFAEMKGVMGDGTGEEVKEEDLGKLPYL 365

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           +AVVLEGLRRHPP HFV PHAVKEDT LENYVIPKNGTVNFMVAEMG DP+VWE+P AFK
Sbjct: 366 RAVVLEGLRRHPPGHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEMGWDPEVWEEPMAFK 425

Query: 382 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 441
           PERFM+G E      FD+TGSKEIKMMPFGAGRR+CP +G+A+LHLEYF+ANLVWRFEWK
Sbjct: 426 PERFMKGGEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRFEWK 485

Query: 442 AVDGDDVDLSEKQELTVVMENPLKAHISSR 464
            V+GD VDLSEK E TVVM+ PLKA+I  R
Sbjct: 486 VVEGDGVDLSEKAEFTVVMDKPLKANIHPR 514

BLAST of Moc02g11580 vs. ExPASy TrEMBL
Match: A0A6J1FY77 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448402 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 7.4e-195
Identity = 338/450 (75.11%), Postives = 385/450 (85.56%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+ + P IFI+DR+IAH AL+ NGA+FA RPPA P+ KI SSNQHNI+SASYGPL
Sbjct: 66  PVVTLRIANRPTIFISDRSIAHKALLQNGALFADRPPAPPMSKIISSNQHNISSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLT QILHPSRV SY  AR WVLDIL+NRL+SHS+S  +PV+VIDHF YAMFCL
Sbjct: 126 WRLLRRNLTFQILHPSRVKSYNRARKWVLDILLNRLVSHSKSE-TPVTVIDHFHYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL+E +I EIE VHRAML+ FRRF VLN WPKLTKILLR+RWE F +++RNQ
Sbjct: 186 LVLMCFGDKLEEPQIHEIENVHRAMLLSFRRFNVLNFWPKLTKILLRKRWEAFLEIRRNQ 245

Query: 202 ENVLIPLIEAQK-------NRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E V+IPLIEA++       NR+   E EE FVVSYVDTLLDL++PDE+NR LTD E+VT+
Sbjct: 246 EKVIIPLIEARRKANQNRANRENRAETEEAFVVSYVDTLLDLELPDEDNRKLTDEEIVTI 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNG-GEEVKEGDLGKLPYL 321
           CSEFL AGTDTTS ALQW MAN+VKYPEIQHKLFAE++GVMG+G GEEVKE DLGKLPYL
Sbjct: 306 CSEFLNAGTDTTSTALQWIMANIVKYPEIQHKLFAEMKGVMGDGTGEEVKEEDLGKLPYL 365

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           +AVVLEGLRRHPP HFV PHAVKEDT LENYVIPKNG+VNFMVAEMG DP+VWEDP AFK
Sbjct: 366 RAVVLEGLRRHPPGHFVLPHAVKEDTMLENYVIPKNGSVNFMVAEMGWDPEVWEDPMAFK 425

Query: 382 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 441
           PERFM+G E      FD+TGSKEIKMMPFGAGRR+CP +G+A+LHLEYF+ANLVWRFEWK
Sbjct: 426 PERFMKGGEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRFEWK 485

Query: 442 AVDGDDVDLSEKQELTVVMENPLKAHISSR 464
           AV+GD VDLSEK E TVVM+ PLKA+I  R
Sbjct: 486 AVEGDGVDLSEKVEFTVVMDKPLKANIHPR 514

BLAST of Moc02g11580 vs. ExPASy TrEMBL
Match: A0A6J1D4C6 (cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017160 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 1.1e-193
Identity = 343/452 (75.88%), Postives = 383/452 (84.73%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+GS P++FIADR IAHAAL+ + ++FA RPPALPV  + SSNQHNI+SASYGPL
Sbjct: 69  PVVTLRIGSRPSVFIADRHIAHAALIQSASLFADRPPALPVSSVVSSNQHNISSASYGPL 128

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLTSQILHPSRVASYG AR WVLDIL+NR LSH ESG +PV V+ HFQYAMFCL
Sbjct: 129 WRLLRRNLTSQILHPSRVASYGGARKWVLDILLNRFLSHPESG-TPVCVVHHFQYAMFCL 188

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFG++L+ETKI+EIE V+RA+L+   RF +L+ +PKLTK+ LR  WE F QL+RNQ
Sbjct: 189 LVLMCFGNRLEETKIREIENVNRALLLQLGRFRILDFFPKLTKLFLRNSWEAFLQLRRNQ 248

Query: 202 ENVLIPLIEAQKN------RDAEIEGEEE-FVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           ENVLIPLIEA++         A+IE EE+ FVVSYVDTLLDLK+ DE NR+LTDGEMV+L
Sbjct: 249 ENVLIPLIEARRKAKQNRANRAQIEKEEDKFVVSYVDTLLDLKLTDEGNRDLTDGEMVSL 308

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYLK 321
           CSEFL AGTDTTS ALQW MANLVKYPEIQ KLFAEIEGVMG G EEVKE DLGKLPYLK
Sbjct: 309 CSEFLNAGTDTTSTALQWIMANLVKYPEIQRKLFAEIEGVMGKGEEEVKEEDLGKLPYLK 368

Query: 322 AVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFKP 381
           AVVLEGLRRHPP HFV PHAVKEDT LENY IPKNGTVNFMVA+MG DP+VWEDP AFKP
Sbjct: 369 AVVLEGLRRHPPGHFVLPHAVKEDTTLENYAIPKNGTVNFMVAQMGWDPKVWEDPMAFKP 428

Query: 382 ERFME--GEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEW 441
           ERFM   G      +AFD+TGSKEIKMMPFGAGRR+CP  GVAMLHLEYF+ANLVWRFEW
Sbjct: 429 ERFMNGGGGGEGETIAFDITGSKEIKMMPFGAGRRMCPGLGVAMLHLEYFIANLVWRFEW 488

Query: 442 KAVDGDDVDLSEKQELTVVMENPLKAHISSRF 465
           KAV GDDVDLSEK E TVVME PLKA ++SRF
Sbjct: 489 KAVAGDDVDLSEKVEFTVVMEKPLKAALNSRF 519

BLAST of Moc02g11580 vs. ExPASy TrEMBL
Match: A0A6J1FV62 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448728 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.3e-191
Identity = 336/451 (74.50%), Postives = 384/451 (85.14%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P +TLR+ + P IFI+DR+IAH AL+ NGA+FA RP A P+ KI SSNQHNI+SASYGPL
Sbjct: 66  PVVTLRIANRPTIFISDRSIAHKALLQNGALFADRPRAPPMSKIISSNQHNISSASYGPL 125

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLT QILHPSR+ SY  AR WVLDIL+NRL+SHS+S  +PV+V+DHFQYAMFCL
Sbjct: 126 WRLLRRNLTFQILHPSRIKSYNRARKWVLDILLNRLVSHSKSE-TPVTVVDHFQYAMFCL 185

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL+E +I EIE VHRAML+ F RF VLN WPKLTKILLR+RWE F +++RNQ
Sbjct: 186 LVLMCFGDKLEEPQIYEIENVHRAMLLGFLRFNVLNFWPKLTKILLRKRWEAFLEIRRNQ 245

Query: 202 ENVLIPLIEAQK-------NRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVTL 261
           E V+IPLIEA++       NR+   E EE+FVVSYVDTLLDL+IPDE+NR LTD E+VT+
Sbjct: 246 EKVIIPLIEARRKANQNRANRENRAETEEDFVVSYVDTLLDLEIPDEDNRKLTDEEIVTI 305

Query: 262 CSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNG-GEEVKEGDLGKLPYL 321
           CSEFL AGTD TS ALQW MAN+VKYPEIQHKL AE++GVMG+G GEEVKE DLGKL YL
Sbjct: 306 CSEFLNAGTDNTSTALQWIMANIVKYPEIQHKLVAEMKGVMGDGTGEEVKEEDLGKLRYL 365

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           +AVVLEGLRRHPPTHFV PHAVKEDT LENYVIPKNGTVNFMVAEMG DP+VWEDP AFK
Sbjct: 366 RAVVLEGLRRHPPTHFVLPHAVKEDTVLENYVIPKNGTVNFMVAEMGWDPEVWEDPMAFK 425

Query: 382 PERFME-GEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEW 441
           PERFM+ GEE      FD+TGSKEIKMMPFGAGRR+CP +G+A+LHLEYF+ANLVWRFEW
Sbjct: 426 PERFMKGGEEEEEVAGFDITGSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRFEW 485

Query: 442 KAVDGDDVDLSEKQELTVVMENPLKAHISSR 464
           KAV+G+ VDLSEK E TVVME PLKA+I  R
Sbjct: 486 KAVEGEGVDLSEKVEFTVVMEKPLKANIIPR 515

BLAST of Moc02g11580 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 568.5 bits (1464), Expect = 4.8e-162
Identity = 282/448 (62.95%), Postives = 346/448 (77.23%), Query Frame = 0

Query: 19  QIRPHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASY 78
           ++ P ITLR+ S P+IF+ADR++AH ALVLNGAVFA RPPA P+ KI SSNQHNI+S  Y
Sbjct: 65  RLGPIITLRITSRPSIFVADRSLAHQALVLNGAVFADRPPAAPISKIISSNQHNISSCLY 124

Query: 79  GPLWRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAM 138
           G  WRLLRRNLTS+ILHPSRV SY  AR WVL+IL +R       G  P+ V+DH  YAM
Sbjct: 125 GATWRLLRRNLTSEILHPSRVRSYSHARRWVLEILFDRF--GKNRGEEPIVVVDHLHYAM 184

Query: 139 FCLLVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLK 198
           F LLVLMCFGDKL E +IK++EYV R  L+ F RF +LNLWPK TK++LR+RWEEFFQ++
Sbjct: 185 FALLVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILNLWPKFTKLILRKRWEEFFQMR 244

Query: 199 RNQENVLIPLI-------EAQKNRDAEIEGE-EEFVVSYVDTLLDLKIPDEENRNLTDGE 258
           R Q +VL+PLI       E +KNR +E E + +E+V SYVDTLL+L++PDE+ R L + E
Sbjct: 245 REQHDVLLPLIRARRKIVEERKNRSSEEEEDNKEYVQSYVDTLLELELPDEK-RKLNEDE 304

Query: 259 MVTLCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKL 318
           +V+LCSEFL  GTDTT+ ALQW MANLVK P+IQ +L+ EI+ V+G    EV+E D  K+
Sbjct: 305 IVSLCSEFLNGGTDTTATALQWIMANLVKNPDIQKRLYEEIKSVVGEEANEVEEEDAQKM 364

Query: 319 PYLKAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPT 378
           PYL+AVV+EGLRRHPP HFV PH+V EDT L  Y +PKNGT+NFMVAE+G DP+VWE+P 
Sbjct: 365 PYLEAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPKVWEEPM 424

Query: 379 AFKPERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRF 438
           AFKPERFME        A D+TGS+ IKMMPFGAGRRICP  G+AMLHLEY+VAN+V  F
Sbjct: 425 AFKPERFMEE-------AVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 484

Query: 439 EWKAVDGDDVDLSEKQELTVVMENPLKA 459
           +WK V G +VDL+EK E TVVM++PLKA
Sbjct: 485 DWKEVQGHEVDLTEKLEFTVVMKHPLKA 502

BLAST of Moc02g11580 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 567.8 bits (1462), Expect = 8.2e-162
Identity = 284/448 (63.39%), Postives = 345/448 (77.01%), Query Frame = 0

Query: 19  QIRPHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASY 78
           ++ P ITLR+ S PAIF+ADR++AH ALVLNGAVFA RPPA P+ KI SSNQHNI+S+ Y
Sbjct: 65  RLGPIITLRITSRPAIFVADRSLAHQALVLNGAVFADRPPAAPISKIISSNQHNISSSLY 124

Query: 79  GPLWRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAM 138
           G  WRLLRRNLTS+ILHPSRV SY  AR WVL+IL +R       G  P+ V+DH  YAM
Sbjct: 125 GATWRLLRRNLTSEILHPSRVRSYSHARRWVLEILFDRF--GKSRGEEPIVVVDHLHYAM 184

Query: 139 FCLLVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLK 198
           F LLVLMCFGDKL E +IK++EYV R  L+ F RF +LNLWPK TK++LR+RWEEFFQ++
Sbjct: 185 FALLVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILNLWPKFTKLILRKRWEEFFQMR 244

Query: 199 RNQENVLIPLI-------EAQKNRDAEIEGEEE-FVVSYVDTLLDLKIPDEENRNLTDGE 258
           R Q +VL+PLI       E +KNR +E E + + +V SYVDTLL+L++PDE+ R L + E
Sbjct: 245 REQHDVLLPLIRARRKIVEERKNRSSEEEEDNKVYVQSYVDTLLELELPDEK-RKLNEDE 304

Query: 259 MVTLCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKL 318
           +V+LCSEFL  GTDTT+ ALQW MANLVK PEIQ +L+ EI+ V+G   +EV+E D  K+
Sbjct: 305 IVSLCSEFLNGGTDTTATALQWIMANLVKNPEIQKRLYEEIKSVVGEEAKEVEEEDAQKM 364

Query: 319 PYLKAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPT 378
           PYLKAVV+EGLRRHPP HFV PH+V EDT L  Y +PK GT+NFMVAE+G DP VWE+P 
Sbjct: 365 PYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRDPMVWEEPM 424

Query: 379 AFKPERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRF 438
           AFKPERFM  EE     A D+TGS+ IKMMPFGAGRRICP  G+AMLHLEY+VAN+V  F
Sbjct: 425 AFKPERFMGEEE-----AVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 484

Query: 439 EWKAVDGDDVDLSEKQELTVVMENPLKA 459
           EWK V G +VDL+EK E TVVM++ LKA
Sbjct: 485 EWKEVQGHEVDLTEKFEFTVVMKHSLKA 504

BLAST of Moc02g11580 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 556.2 bits (1432), Expect = 2.5e-158
Identity = 277/445 (62.25%), Postives = 339/445 (76.18%), Query Frame = 0

Query: 22  PHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASYGPL 81
           P ITLR+ S PAIF+ DR++AH ALVLNGAVFA RPPA  + KI SSNQHNI+S  YG  
Sbjct: 69  PIITLRITSRPAIFVTDRSLAHQALVLNGAVFADRPPAESISKIISSNQHNISSCLYGAT 128

Query: 82  WRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAMFCL 141
           WRLLRRNLTS+ILHPSR+ SY  AR WVL+IL  R       G  P+ V+DH  YAMF L
Sbjct: 129 WRLLRRNLTSEILHPSRLRSYSHARRWVLEILFGRF--GKNRGEEPIVVVDHLHYAMFAL 188

Query: 142 LVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLKRNQ 201
           LVLMCFGDKL E +IK++EYV R  L+ F RF +L LWPK TK++ R+RWEEFFQ++  Q
Sbjct: 189 LVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILTLWPKFTKLIYRKRWEEFFQMQSEQ 248

Query: 202 ENVLIPLIEA-------QKNRDAEIEGE-EEFVVSYVDTLLDLKIPDEENRNLTDGEMVT 261
           ++VL+PLI A       +K R +E E + +E+V SYVDTLLD+++PDE+ R L + E+V+
Sbjct: 249 QDVLLPLIRARRKIVDERKKRSSEEEKDNKEYVQSYVDTLLDVELPDEK-RKLNEDEIVS 308

Query: 262 LCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYL 321
           LCSEFL AGTDTT+ ALQW MANLVK PEIQ +L+ EI+ ++G   +EV+E D  K+PYL
Sbjct: 309 LCSEFLNAGTDTTATALQWIMANLVKNPEIQRRLYEEIKSIVGEEAKEVEEQDAQKMPYL 368

Query: 322 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 381
           KAVV+EGLRRHPP HFV PH+V EDT L  Y +PK GT+NFMVAE+G DP+VWE+P AFK
Sbjct: 369 KAVVMEGLRRHPPGHFVLPHSVTEDTVLGGYKVPKKGTINFMVAEIGRDPKVWEEPMAFK 428

Query: 382 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 441
           PERFME        A D+TGS+ IKMMPFGAGRRICP  G+AMLHLEY+VAN+V  FEW+
Sbjct: 429 PERFMEE-------AVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFEWQ 488

Query: 442 AVDGDDVDLSEKQELTVVMENPLKA 459
            V G +VDL+EK E TVVM++PLKA
Sbjct: 489 EVQGHEVDLTEKLEFTVVMKHPLKA 503

BLAST of Moc02g11580 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 548.1 bits (1411), Expect = 6.7e-156
Identity = 268/445 (60.22%), Postives = 341/445 (76.63%), Query Frame = 0

Query: 19  QIRPHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASY 78
           ++ P +TLR+ S PAIF+ADR++ H ALVLNGAV+A RPP   + KI   ++HNI+S SY
Sbjct: 64  RLGPIVTLRITSRPAIFVADRSLTHEALVLNGAVYADRPPPAVISKIV--DEHNISSGSY 123

Query: 79  GPLWRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAM 138
           G  WRLLRRN+TS+ILHPSRV SY  AR+WVL+IL  R  +H   G  P+ +I H  YAM
Sbjct: 124 GATWRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNH--GGEEPIVLIHHLHYAM 183

Query: 139 FCLLVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLK 198
           F LLVLMCFGDKL E +IKE+E++ R  L+   +F + N+WPK TK++LR+RW+EF Q++
Sbjct: 184 FALLVLMCFGDKLDEKQIKEVEFIQRLQLLSLTKFNIFNIWPKFTKLILRKRWQEFLQIR 243

Query: 199 RNQENVLIPLIEA-----QKNRDAEIEGEEEFVVSYVDTLLDLKIPDEENRNLTDGEMVT 258
           R Q +VL+PLI A     ++ + +E E ++++V SYVDTLLDL++P EENR L + +++ 
Sbjct: 244 RQQRDVLLPLIRARRKIVEERKRSEQEDKKDYVQSYVDTLLDLELP-EENRKLNEEDIMN 303

Query: 259 LCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKLPYL 318
           LCSEFLTAGTDTT+ ALQW MANLVKYPEIQ +L  EI+ V+G   +EV+E D+ K+PYL
Sbjct: 304 LCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVVGEEAKEVEEEDVEKMPYL 363

Query: 319 KAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPTAFK 378
           KAVVLEGLRRHPP HF+ PH+V EDT L  Y +PKNGT+NFMVAE+G DP  WE+P AFK
Sbjct: 364 KAVVLEGLRRHPPGHFLLPHSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFK 423

Query: 379 PERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRFEWK 438
           PERFM  EE     A D+TGS+ IKMMPFGAGRRICP  G+AMLHLEY+VAN+V  F+WK
Sbjct: 424 PERFMGEEE-----AVDLTGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWK 483

Query: 439 AVDGDDVDLSEKQELTVVMENPLKA 459
            V G +VDL+EK E TVVM++PLKA
Sbjct: 484 EVQGHEVDLTEKLEFTVVMKHPLKA 498

BLAST of Moc02g11580 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 531.6 bits (1368), Expect = 6.5e-151
Identity = 268/448 (59.82%), Postives = 338/448 (75.45%), Query Frame = 0

Query: 19  QIRPHITLRVGSHPAIFIADRTIAHAALVLNGAVFAHRPPALPVRKIASSNQHNINSASY 78
           ++ P ITLR+ S PAIF+AD ++AH ALVLNGAVFA RPPA P+ KI S+NQH I S  Y
Sbjct: 65  RLGPIITLRITSRPAIFVADGSLAHQALVLNGAVFADRPPAAPISKILSNNQHTITSCLY 124

Query: 79  GPLWRLLRRNLTSQILHPSRVASYGEARNWVLDILINRLLSHSESGFSPVSVIDHFQYAM 138
           G  WRLLRRN+T +ILHPSR+ SY   R+WVL+IL +RL      G  P+ V DH  YAM
Sbjct: 125 GVTWRLLRRNIT-EILHPSRMKSYSHVRHWVLEILFDRL--RKSGGEEPIVVFDHLHYAM 184

Query: 139 FCLLVLMCFGDKLKETKIKEIEYVHRAMLVDFRRFEVLNLWPKLTKILLRRRWEEFFQLK 198
           F +LVLMCFGDKL E +IK++EYV R ML+ F R+ +LNL PK TK++LR+RWEEFFQ++
Sbjct: 185 FAVLVLMCFGDKLDEKQIKQVEYVQRQMLLGFARYSILNLCPKFTKLILRKRWEEFFQMR 244

Query: 199 RNQENVLIPLI-------EAQKNRDAEIEGE-EEFVVSYVDTLLDLKIPDEENRNLTDGE 258
           R Q++VL+ LI       E +K R +E E E +E+V SYVDTLLD+++PDE+ R L + E
Sbjct: 245 REQQDVLLRLIYARRKIVEERKKRSSEEEEENKEYVQSYVDTLLDVELPDEK-RKLNEDE 304

Query: 259 MVTLCSEFLTAGTDTTSIALQWTMANLVKYPEIQHKLFAEIEGVMGNGGEEVKEGDLGKL 318
           +V+LCSEFL AG+DTT+  LQW MANLVK  EIQ +L+ EI  V+G   + V+E D  K+
Sbjct: 305 IVSLCSEFLIAGSDTTATVLQWIMANLVKNQEIQERLYEEITNVVGEEAKVVEEKDTQKM 364

Query: 319 PYLKAVVLEGLRRHPPTHFVPPHAVKEDTNLENYVIPKNGTVNFMVAEMGLDPQVWEDPT 378
           PYLKAVV+E LRRHPP + V PH+V EDT L  Y +PK GT+NF+VAE+G DP+VWE+P 
Sbjct: 365 PYLKAVVMEALRRHPPGNTVLPHSVTEDTVLGGYKVPKKGTINFLVAEIGRDPKVWEEPM 424

Query: 379 AFKPERFMEGEETAAALAFDVTGSKEIKMMPFGAGRRICPAYGVAMLHLEYFVANLVWRF 438
           AFKPERFM  EE     A D+TGS+ IKMMPFGAGRRICP  G+AMLHLEY+VAN+V  F
Sbjct: 425 AFKPERFMGEEE-----AVDITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREF 484

Query: 439 EWKAVDGDDVDLSEKQELTVVMENPLKA 459
           +WK V+G +VDL+EK E TV+M++PLKA
Sbjct: 485 QWKEVEGHEVDLTEKVEFTVIMKHPLKA 503

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148526.12.6e-20280.00cytochrome P450 89A2-like [Momordica charantia][more]
XP_022986504.12.1e-19675.56cytochrome P450 89A2-like [Cucurbita maxima][more]
XP_022943745.11.5e-19475.11cytochrome P450 89A2-like [Cucurbita moschata][more]
KAG7010999.17.6e-19475.11Cytochrome P450 89A2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022148533.12.2e-19375.88cytochrome P450 89A2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q426029.4e-15560.22Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2[more]
Q9SRQ11.4e-13753.88Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1[more]
P371232.0e-8840.90Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=... [more]
O489287.1e-8639.37Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1[more]
Q9LZ313.9e-8439.42Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D5N11.3e-20280.00cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017152 PE=3 SV... [more]
A0A6J1JG801.0e-19675.56cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111484223 PE=3 SV=1[more]
A0A6J1FY777.4e-19575.11cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448402 PE=3 SV=... [more]
A0A6J1D4C61.1e-19375.88cytochrome P450 89A2-like OS=Momordica charantia OX=3673 GN=LOC111017160 PE=3 SV... [more]
A0A6J1FV621.3e-19174.50cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448728 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G64950.14.8e-16262.95cytochrome P450, family 89, subfamily A, polypeptide 5 [more]
AT2G12190.18.2e-16263.39Cytochrome P450 superfamily protein [more]
AT1G64940.12.5e-15862.25cytochrome P450, family 87, subfamily A, polypeptide 6 [more]
AT1G64900.16.7e-15660.22cytochrome P450, family 89, subfamily A, polypeptide 2 [more]
AT1G64930.16.5e-15159.82cytochrome P450, family 87, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 315..326
score: 40.67
coord: 400..409
score: 60.88
coord: 261..278
score: 40.39
IPR001128Cytochrome P450PFAMPF00067p450coord: 20..455
e-value: 3.7E-78
score: 263.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 41..62
score: 26.16
coord: 17..36
score: 19.73
coord: 399..409
score: 58.26
coord: 250..267
score: 29.8
coord: 409..432
score: 25.91
coord: 270..296
score: 37.17
coord: 355..379
score: 33.24
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 13..464
e-value: 5.9E-97
score: 327.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 16..463
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 21..461
NoneNo IPR availablePANTHERPTHR24298:SF850CYTOCHROME P450coord: 21..461
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 402..411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g11580.1Moc02g11580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen