Moc02g11560 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g11560
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Aminoacyl-tRNA hydrolase
Location	chr2: 8343395 .. 8347090 (-)
RNA-Seq Expression	Moc02g11560
Synteny	Moc02g11560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACATAAGCGTCATCATTCAATAACTGGTAATGCTTTTTGTGACATCTTTTATAGTTCGTTTCCAGAAAGGAAAAAGCTTTTCTGCAATTAAGTTTCCATCAAGAACTGCATTAGCTGTGACACAACGAAGAGCCGATGTTGTCAATTAGAAGTTGAATCCATGGGAATTTAATTTGAAGTATCTTTGCTAAGAAATCGGTAATTTTATGACAGTAACAAAAAATATGGTAGGGTTATTTTGCAGCGACGGCTTCTTTGATTGATTTAACCTTTTTTTTTAACACCTTTGTTAATTTGCTTTTTGTGAAGCGTACTATAGAATCTGATCGCTTAGTTCTGAAGCTGTTTTAAGCTTCTATAGTTAGTAATTGGTACGTTCATGGTCTATTGCTAGATTGATTACTTATTGCAATATTGGGGTTTCAGAAAATTGAATCTCGAACTGGCTTTCATAATGCTTATACTGTTCTCTGAGAGGCAAAAGCAAGCAGAATTCAAAATTTTTGTTGTATTCCATAATCCTGATTCTGAATTTGAGGAAATGTCTGTTATTTTCTTTATTTTATCTTTGATTTTTTTTTATCCTTCGATTGGTCTTTTCTTCCTGTCCTTGCTGTCTCTGAGTTTTAATTTTGTGCTTTTCCTGTTCTATATAGGTTTTCTATTGCTTGAATTAGAATTTGGTATCTGGTCCAGCATTATACTGGCTGGACTTAAGTAGTGGAGGAGTGCATTTTATTGGCTAAGATTGGATGGATCTGACATGGCTGAGTGCCATTGTAGTTGGGGCCGGCTGCCTGGTTTTGGGATACTATATTGGTTCAAAGTATCCTCCTCGCATATTTTTCAAAGCCAAATTAGCCAAAAGCAACGAATCTAGTAGAAGTGGGAAGAAGGATAAGCCTAAAGAGGCACTTGAGATTGAAAGTTTGGCTGACATTCTCGATGACTTCAAGATGGTCTGTTTCTGTGTCTCTCTGTCTCTGTCTATCCATTTGAAAATATGGCTGACATTCACGATGACTTCAAGATGGTCTGTCTCTCTCTCGAAAAAATTAAATAACATTTTACTATATCTCTGCAGGTTTTGGTTGTGAGAAATGATTTGAAAATGGGCAAAGGAAAAATTGCTGCCCAGTGCAGGTATTTCTTACCAACTTGTGATTGCTCTTTCGTTATTTGATGTTTTGAATGCTAATAATCACGAAAACTTTAAATTTCCCTGTTGCTTTCCGAGTGTGGAAATACGAAGTTTGTTTTTGTTAAAAACAAATCGTAATAATTGGGCATATAGTGTTTTATAAGTTTATATTAGCTACCCAATGCAGCCACCTGGTGTAGTAAATTTCTGATAATGACTACAATAACATAATATTAATTACTATAATAAAGTTTAGTAAAATTTAGTTGTATAGATGCATATCAAATATCAGTGATTATAATGACATGTGAGGGTGAGGGGGAGAGGTTCAAGTGAAAGTTGTCATAATGCAAAACTTGGATGATAATGGTGATTCTAATTTTTTAAAATTACATGTTAAAATCAGGAAAATTATTGATGAATGGTATCTAAAAAGCTATAGCAAAGGTGCTAATGGGAAGAACTCTGTCAAGCTTAGACAAATTCTTGATGTCACTTTAATTGCAAATGAAACGGTGGAGGAAGAGAGATGTAAGAAAGAAAAAAAATTAGGTGATTCAAATAGATGTAGCAAAAGTCTACAATAAAGTGTGTCTATAATTTTTGGATAGAGTGTTGAAAGTATAGTAGGCTTGAGAAGGTGGATAAGAGGGTGCGTCATCAGTAGGTCTTCTTTCTAAGAAGAAAATAGAAGCTTCAATGGGATTGAGGCAAGAGATTACATATTCTAATGATGCAAACGAAGATGTTTCATTAGTATTACATAGATAGTTTGGATCATTATATGGATGAGAACCAAACTTCAATGTCTTGTTCTACCATCAAAAAGAACTCATATTACGTGATTGCAATTATCAGCAAACCTTGCGGCAGATATTTACTGAAAGGATCGCCTATGCAACACAATCATTTTAAATTACAATGTGAAAGGCCGAGTTTTCTTATTTTCTAATATGTGTATGACTAAATGTGTACTGCCTTATTACCTGTTTCGTAGTAAAATTATTGAGAATTGAGACTAGTAATCTAATTTTCTCAAACCTTTGCACCATTAACTTTCCAGTTTCTTAATGCTGTAAATTGGATTTGGACTAGTTTATAATCTACTTTACTTGCTTTTGTTAGTCACGCAACTTTGGGTCTCTATAAGAAGCTCCATCATCGTGCACCGAAAGCTTTGAATAGGTATGTATGTATGAACCGAACAGAATAGTTCTCCAGATATTAATGTGCATCGTGTACTTTGTTGTCTGCACATTTATTTGATTTTATTACATGTAGGTGGGAGATGTGTGCACAGCCCAAGGTGGTTCTGAAAATAGAGAGTGAGGAAGACATGCTAGTTTTGCAAGTAACAGTTCCACTATGATTTTCTTTCCTTAAAAATCTCTCCATGATTTTATAGTATCTAGACGATTTCTTTTTTGTGTGCACTTTGTGGTGCCATCAGTATCTGCCTTATTATGTCAAAAGAATATAACATTTACAGTTGGTTGATGTCTGTTAGTTTATATATATATATAATTCAATTTTGATTATGAGTGCAAGCGGGTCAATTTGGGTTACTTTCTTGCTGAAACCAATATTTTTGCAAACCGATTAAAACAAAACCGAACTCACTATTTTTTATTTTATACTTTAGAAGACCGAACAAATCAACAAGCTTACTTCAAACCAACCAAACGGTCTGGTTGATTGGTTTTTTTCTTTCAATTTTTGGTTGTACCCCTACCTCTAGGCTCTAATGCCCTTCATATTTATTAAGACATTTGTTCTTTTACCCCCCTTCCCTCTTTTGTAGAATGCTCATTTCATCAGATCTTATTTTTCTTGCTGACTTTACTTAGTCGGCCATAAAATTTCAAAGAGGATAGTATTTTGAAATTTTCTCGTGTCGGATAGTCATCTATGTAGTTAATATCATTGGCAAAGAGGGGAGATGGAGAAAAATTGACTGTTTAATTCTTGGTACTTTTGGCCCTTGATCTTGGTTCTTTTATTTTTCAAATTTATATGTTTTCTTGTTCATCAGGAGAGGGCAAAATCATTGAAGTTACCAACCCATATTACCATTGATGCTGGAAGAACTCAGATTGCTCCAAGTAAGTTCTACAAAATTCACTTAAATTCATCACTTTTTCCATAGCAAATTTATCAAGTTCACTAAGTTCTGCAATTCCGTTCTTCCAGATTCAAGAACGGTGATGGCAGTACTCGGTGAGTGGCTTTTTTCCATGTATCATTTATATAATCAAAGAGTCTTTTCATTGTCGCCTATACAATTTAAGCATTCCTTTTTAGCAAAATATCTCGTGCGTATAAAATTCCTTTGGCCCCTCCCTTTTTGTTCTTGCCTCATTTTTTCGTCGGCTGGTGTATCTACTAAACAGTACCATTGTGGTGCATTCCAACTGAAATTAATTAAACTAATTAGGTTCACCTAATGAACGTATTAGTAGTAGTGCATAGTTGAATACACTTCTCATTTCACAATATGCAGGTCCCGTTGAAGTGGTTGATGATGTAACTGGTGGGTTGAAACTCTTGTAG

mRNA sequence

ATGCAACATAAGCGTCATCATTCAATAACTGTTGGGGCCGGCTGCCTGGTTTTGGGATACTATATTGGTTCAAAGTATCCTCCTCGCATATTTTTCAAAGCCAAATTAGCCAAAAGCAACGAATCTAGTAGAAGTGGGAAGAAGGATAAGCCTAAAGAGGCACTTGAGATTGAAAGTTTGGCTGACATTCTCGATGACTTCAAGATGGTTTTGGTTGTGAGAAATGATTTGAAAATGGGCAAAGGAAAAATTGCTGCCCAGTGCAGTCACGCAACTTTGGGTCTCTATAAGAAGCTCCATCATCGTGCACCGAAAGCTTTGAATAGGTGGGAGATGTGTGCACAGCCCAAGGTGGTTCTGAAAATAGAGAGTGAGGAAGACATGCTAGTTTTGCAAGAGAGGGCAAAATCATTGAAGTTACCAACCCATATTACCATTGATGCTGGAAGAACTCAGATTGCTCCAAATTCAAGAACGGTGATGGCAGTACTCGGTCCCGTTGAAGTGGTTGATGATGTAACTGGTGGGTTGAAACTCTTGTAG

Coding sequence (CDS)

ATGCAACATAAGCGTCATCATTCAATAACTGTTGGGGCCGGCTGCCTGGTTTTGGGATACTATATTGGTTCAAAGTATCCTCCTCGCATATTTTTCAAAGCCAAATTAGCCAAAAGCAACGAATCTAGTAGAAGTGGGAAGAAGGATAAGCCTAAAGAGGCACTTGAGATTGAAAGTTTGGCTGACATTCTCGATGACTTCAAGATGGTTTTGGTTGTGAGAAATGATTTGAAAATGGGCAAAGGAAAAATTGCTGCCCAGTGCAGTCACGCAACTTTGGGTCTCTATAAGAAGCTCCATCATCGTGCACCGAAAGCTTTGAATAGGTGGGAGATGTGTGCACAGCCCAAGGTGGTTCTGAAAATAGAGAGTGAGGAAGACATGCTAGTTTTGCAAGAGAGGGCAAAATCATTGAAGTTACCAACCCATATTACCATTGATGCTGGAAGAACTCAGATTGCTCCAAATTCAAGAACGGTGATGGCAGTACTCGGTCCCGTTGAAGTGGTTGATGATGTAACTGGTGGGTTGAAACTCTTGTAG

Protein sequence

MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL

Homology

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BLAST of Moc02g11560 vs. NCBI nr
Match: XP_022148653.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Momordica charantia])

HSP 1 Score: 355.9 bits (912), Expect = 2.0e-94
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60
           MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL
Sbjct: 1   MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL 120
           ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180

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BLAST of Moc02g11560 vs. NCBI nr
Match: XP_022148652.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Momordica charantia])

HSP 1 Score: 335.9 bits (860), Expect = 2.2e-88
Identity = 171/173 (98.84%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 8   SITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67
           +I VGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF
Sbjct: 8   AIVVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67

Query: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED
Sbjct: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127

Query: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL
Sbjct: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180

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BLAST of Moc02g11560 vs. NCBI nr
Match: XP_022986616.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 321.6 bits (823), Expect = 4.2e-84
Identity = 163/181 (90.06%), Postives = 171/181 (94.48%), Query Frame = 0


Query: 1   MQHKRH-HSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIES 60
           MQHK + HS  VGA CLVLGY IGSKYPPRIF KAKLAK N+SSR+GKKDKPKE LE+E+
Sbjct: 1   MQHKHNRHSTPVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMC QPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCGQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKL 180
           LKIESEED+LVLQERAKSLKLPTHITIDAG+TQIAPNSRTVMA+LGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDILVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

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BLAST of Moc02g11560 vs. NCBI nr
Match: KAG7035146.1 (Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 319.7 bits (818), Expect = 1.6e-83
Identity = 162/173 (93.64%), Postives = 168/173 (97.11%), Query Frame = 0


Query: 8   SITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67
           +I VGAGCLVLGY IGSKYPPRIF KAKLAKSNESSRSGKK KPKE LE+E+LADIL+DF
Sbjct: 8   AIVVGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRSGKKGKPKEPLEVENLADILEDF 67

Query: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMCAQPKVVLKIESEED
Sbjct: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESEED 127

Query: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMA+LGPVEVVDDVTGGLKLL
Sbjct: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

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BLAST of Moc02g11560 vs. NCBI nr
Match: XP_023511827.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 319.7 bits (818), Expect = 1.6e-83
Identity = 162/181 (89.50%), Postives = 171/181 (94.48%), Query Frame = 0


Query: 1   MQHKRH-HSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIES 60
           MQHK + HS  VGA CLVLGY IGSKYPPRIF KA+LAK N+SSR+GKKDKPKE LE+E+
Sbjct: 1   MQHKHNRHSTPVGASCLVLGYCIGSKYPPRIFIKARLAKINQSSRNGKKDKPKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMC QPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAG+TQIAPNSRTVMA+LGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

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BLAST of Moc02g11560 vs. ExPASy Swiss-Prot
Match: Q9Y3E5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.8e-30
Identity = 72/137 (52.55%), Postives = 91/137 (66.42%), Query Frame = 0


Query: 47  KKDKPKEALEIESLADILDD---FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRA 106
           K    K   + ES A IL D   +KM+LVVRNDLKMGKGK+AAQCSHA +  YK++  R 
Sbjct: 42  KSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRN 101

Query: 107 PKALNRWEMCAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAV 166
           P+ L +WE C QPKVV+K   EE ++ L   AK L L   +  DAGRTQIAP S+TV+ +
Sbjct: 102 PEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGI 161

Query: 167 -LGPVEVVDDVTGGLKL 180
             GP +++D VTG LKL
Sbjct: 162 GPGPADLIDKVTGHLKL 178

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BLAST of Moc02g11560 vs. ExPASy Swiss-Prot
Match: Q8R2Y8 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.8e-29
Identity = 74/174 (42.53%), Postives = 106/174 (60.92%), Query Frame = 0


Query: 8   SITVGAGC-LVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDD 67
           S+  G  C + LG+  G +    +F +   +++N  + +G +          S+     +
Sbjct: 18  SLAAGVACGMCLGW--GLRSHLGMFPQNSTSEANRDTETGTE---------ASILGESGE 77

Query: 68  FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEE 127
           +KM+LVVR DLKMGKGK+AAQCSHA +  YK+   R+P+ L  WE C QPKVV+K   E+
Sbjct: 78  YKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDED 137

Query: 128 DMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAV-LGPVEVVDDVTGGLKL 180
            ++ L   AK+L L   +  DAGRTQI P SRTV+ +  GPVE++D+VTG LKL
Sbjct: 138 TLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKL 180

[Close]

BLAST of Moc02g11560 vs. ExPASy Swiss-Prot
Match: Q3ZBL5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.0e-29
Identity = 79/174 (45.40%), Postives = 103/174 (59.20%), Query Frame = 0


Query: 8   SITVGAGC-LVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDD 67
           S+  G  C + LG+ +  ++         L KS  S R    D   EA    S+     +
Sbjct: 18  SLAAGVACGVCLGWGLRMRF-------GMLPKS--SVRETNPDTETEA----SILGESGE 77

Query: 68  FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEE 127
           +KM+LVVRNDLKMGKGK+AAQCSHA +  YK++  R P+ L  WE C QPKVV+K   EE
Sbjct: 78  YKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEYCGQPKVVVKAPDEE 137

Query: 128 DMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAV-LGPVEVVDDVTGGLKL 180
            ++ L   AK L L   +  DAGRTQIAP SRTV+ +  GP +++D VTG LKL
Sbjct: 138 TLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVLGIGPGPADLIDKVTGHLKL 178

[Close]

BLAST of Moc02g11560 vs. ExPASy Swiss-Prot
Match: O97067 (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 PE=2 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 1.6e-25
Identity = 71/174 (40.80%), Postives = 105/174 (60.34%), Query Frame = 0


Query: 16  LVLGYYIGSKYPPR-------IFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDFK 75
           ++L +++G +Y  +       +   A    S ESS S   +    ++  +    + D+FK
Sbjct: 17  VMLSFFVGYRYALKRGDAKDSVTEGAATPFSQESSVSSGSE---ASVSDKGYGGLNDNFK 76

Query: 76  MVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEEDM 135
           MVLVVRNDLKMGKGKIAAQC H  +G Y++   R P+ L  WE C   K+ +++ESE ++
Sbjct: 77  MVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRVESEAEL 136

Query: 136 LVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEV--VDDVTGGLKLL 181
           + +++ A+  +L T +  DAGRTQI  NS+TV+AV GP     +D VTG LKLL
Sbjct: 137 MAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAV-GPAAAADIDRVTGHLKLL 186

[Close]

BLAST of Moc02g11560 vs. ExPASy Swiss-Prot
Match: O13830 (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.2e-22
Identity = 74/192 (38.54%), Postives = 103/192 (53.65%), Query Frame = 0


Query: 8   SITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEAL---EIESLADIL 67
           ++ VGA   V+G+ IG KY      +   +K+   + + +K+ P       + ES  ++L
Sbjct: 16  AVLVGA---VVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESETELL 75

Query: 68  D----------------DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALN 127
           D                  KMVLVVR DL M KGKIAAQC+HA L  YK      P  + 
Sbjct: 76  DMLKGNSSLAALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPDLVR 135

Query: 128 RWEMCAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAV-LGPV 180
            WE   Q K+ L+ ++EE + +LQ +A SL L   +  DAGRTQIA  S TV+ +  GPV
Sbjct: 136 IWENAGQAKITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGPGPV 195

[Close]

BLAST of Moc02g11560 vs. ExPASy TrEMBL
Match: A0A6J1D4P0 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 9.8e-95
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60
           MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL
Sbjct: 1   MQHKRHHSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL 120
           ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180

[Close]

BLAST of Moc02g11560 vs. ExPASy TrEMBL
Match: A0A6J1D5N0 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.1e-88
Identity = 171/173 (98.84%), Postives = 172/173 (99.42%), Query Frame = 0


Query: 8   SITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67
           +I VGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF
Sbjct: 8   AIVVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67

Query: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED
Sbjct: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127

Query: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL
Sbjct: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180

[Close]

BLAST of Moc02g11560 vs. ExPASy TrEMBL
Match: A0A6J1JBP2 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.1e-84
Identity = 163/181 (90.06%), Postives = 171/181 (94.48%), Query Frame = 0


Query: 1   MQHKRH-HSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIES 60
           MQHK + HS  VGA CLVLGY IGSKYPPRIF KAKLAK N+SSR+GKKDKPKE LE+E+
Sbjct: 1   MQHKHNRHSTPVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMC QPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCGQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKL 180
           LKIESEED+LVLQERAKSLKLPTHITIDAG+TQIAPNSRTVMA+LGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDILVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

[Close]

BLAST of Moc02g11560 vs. ExPASy TrEMBL
Match: A0A6J1FZ05 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.0e-83
Identity = 162/181 (89.50%), Postives = 171/181 (94.48%), Query Frame = 0


Query: 1   MQHKRH-HSITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIES 60
           MQHK + HS  VGA CLVLGY IGSKYPPRIF KAKLAK N+SSR+GKKDKPKE LE+E+
Sbjct: 1   MQHKHNRHSTPVGASCLVLGYCIGSKYPPRIFIKAKLAKINQSSRNGKKDKPKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLK+GKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMC QPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKIGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCGQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAG+TQIAPNSRTVMA+LGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGKTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

[Close]

BLAST of Moc02g11560 vs. ExPASy TrEMBL
Match: A0A6J1G686 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.7e-83
Identity = 161/173 (93.06%), Postives = 168/173 (97.11%), Query Frame = 0


Query: 8   SITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67
           +I VGAGCLVLGY IGSKYPPRIF KAKLAKSNESSR+GKK KPKE LE+E+LADIL+DF
Sbjct: 8   AIVVGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRTGKKGKPKEPLEVENLADILEDF 67

Query: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMCAQPKVVLKIESEED
Sbjct: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESEED 127

Query: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMA+LGPVEVVDDVTGGLKLL
Sbjct: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

[Close]

BLAST of Moc02g11560 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 236.1 bits (601), Expect = 2.2e-62
Identity = 127/173 (73.41%), Postives = 141/173 (81.50%), Query Frame = 0


Query: 9   ITVGAGCLVLGYYIGS-KYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 68
           + VGA    LGYYI + +   RIF  +K    +  S   KK K KE LEIE LAD   +F
Sbjct: 10  LLVGA---ALGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIEKLADFRKNF 69

Query: 69  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 128
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKALNRWE CAQPKVV+KIESEE+
Sbjct: 70  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKVVVKIESEEE 129

Query: 129 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           MLVLQERAK+LKLPTHITIDAG+TQIAPNSRTVMA+LGPV+ VD+VTGGLKL+
Sbjct: 130 MLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMAILGPVDNVDEVTGGLKLM 179

[Close]

BLAST of Moc02g11560 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 236.1 bits (601), Expect = 2.2e-62
Identity = 127/173 (73.41%), Postives = 141/173 (81.50%), Query Frame = 0


Query: 9   ITVGAGCLVLGYYIGS-KYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 68
           + VGA    LGYYI + +   RIF  +K    +  S   KK K KE LEIE LAD   +F
Sbjct: 10  LLVGA---ALGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIEKLADFRKNF 69

Query: 69  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 128
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKALNRWE CAQPKVV+KIESEE+
Sbjct: 70  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKVVVKIESEEE 129

Query: 129 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           MLVLQERAK+LKLPTHITIDAG+TQIAPNSRTVMA+LGPV+ VD+VTGGLKL+
Sbjct: 130 MLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMAILGPVDNVDEVTGGLKLM 179

[Close]

BLAST of Moc02g11560 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 224.9 bits (572), Expect = 5.0e-59
Identity = 124/173 (71.68%), Postives = 137/173 (79.19%), Query Frame = 0


Query: 8   SITVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESLADILDDF 67
           ++ VGA    LGY IG++    +          +SS+S         LEIE LADILDDF
Sbjct: 8   ALIVGA---ALGYCIGTRRSKPVVAAVDKDIKTQSSKS--------PLEIEKLADILDDF 67

Query: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESEED 127
           KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKAL+ WE CAQPKVV+KIE E++
Sbjct: 68  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVVKIEDEDE 127

Query: 128 MLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 181
           ML LQERAKSLKLPTHITIDAGRTQIAPNSRTVMA+LGPVEVVD+VTGGLKLL
Sbjct: 128 MLELQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDEVTGGLKLL 169

[Close]

BLAST of Moc02g11560 vs. TAIR 10
Match: AT4G32900.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 111.7 bits (278), Expect = 6.2e-25
Identity = 59/116 (50.86%), Postives = 80/116 (68.97%), Query Frame = 0


Query: 66  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESE 125
           + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L       L RWE   QPK+V+  +++
Sbjct: 78  ELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQ 137

Query: 126 EDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAV-LGPVEVVDDVTGGLKLL 181
           ++M  + E A+S+ LPT +  DAGRT++A  SRTV+AV  GP E+VD +TG L LL
Sbjct: 138 QEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLAVGPGPKELVDSITGRLALL 193

[Close]

BLAST of Moc02g11560 vs. TAIR 10
Match: AT4G32900.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.3e-11
Identity = 31/63 (49.21%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 66  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVLKIESE 125
           + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L       L RWE   QPK+V+  +++
Sbjct: 78  ELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQ 137

Query: 126 EDM 129
           ++M
Sbjct: 138 QEM 140

The following BLAST results are available for this feature:

BLAST of Moc02g11560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148653.1	2.0e-94	100.00	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X2 [Momordica charantia]	[more]
XP_022148652.1	2.2e-88	98.84	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Momordica charantia]	[more]
XP_022986616.1	4.2e-84	90.06	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita maxima]	[more]
KAG7035146.1	1.6e-83	93.64	Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_023511827.1	1.6e-83	89.50	peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. ...	[more]

BLAST of Moc02g11560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y3E5	2.8e-30	52.55	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 S...	[more]
Q8R2Y8	1.8e-29	42.53	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 ...	[more]
Q3ZBL5	4.0e-29	45.40	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=...	[more]
O97067	1.6e-25	40.80	Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 ...	[more]
O13830	2.2e-22	38.54	Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]

BLAST of Moc02g11560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4P0	9.8e-95	100.00	Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=...	[more]
A0A6J1D5N0	1.1e-88	98.84	Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=...	[more]
A0A6J1JBP2	2.1e-84	90.06	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484304 PE=4 SV=1	[more]
A0A6J1FZ05	1.0e-83	89.50	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449121 PE=4 SV=1	[more]
A0A6J1G686	1.7e-83	93.06	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1	[more]

BLAST of Moc02g11560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03010.1	2.2e-62	73.41	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.2	2.2e-62	73.41	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT5G16870.1	5.0e-59	71.68	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT4G32900.2	6.2e-25	50.86	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT4G32900.1	2.3e-11	49.21	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 68..180 e-value: 1.4E-41 score: 141.5
IPR002833	Peptidyl-tRNA hydrolase, PTH2	TIGRFAM	TIGR00283	TIGR00283	coord: 68..180 e-value: 1.2E-33 score: 113.8
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PANTHER	PTHR12649	PEPTIDYL-TRNA HYDROLASE 2	coord: 6..180
IPR002833	Peptidyl-tRNA hydrolase, PTH2	CDD	cd02430	PTH2	coord: 67..180 e-value: 1.01366E-62 score: 187.346
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 65..180 e-value: 3.1E-44 score: 151.7
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 65..180
None	No IPR available	PANTHER	PTHR12649:SF19	OSJNBA0060D06.11 PROTEIN	coord: 6..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g11560.1	Moc02g11560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity