Moc02g10880 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g10880
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Locationchr2: 7761336 .. 7761883 (+)
RNA-Seq ExpressionMoc02g10880
SyntenyMoc02g10880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTTCTCTGCAGATCCCCCGCGTTCCCCATCGCGCAGCCTTCATTTCACCCAGTCCCCTCTCCGCCGCCGGAGCTCTGCCTCTTCGCCTTCGGAAACCCTTTTCCCTCCGGTGCACCGCCGATTCTCCGTCTTCTCCGGGGCCGCTCGATCCCGATTTCGACGCGAAGTTGTTCTGGAAGAACTGGGCCAGAGGTGAAAACTACAACCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGAAGCTCATGAACAGCGAGTTCACGAGTGAGTTTTCTTGTATTCGATTGTACCAATATCGTGATGGTTTTAAACGATTTCTGTGTTGAATGGCTCAGGTGAAGTTATAAATACTCTGAATGAAAATGGGAACGAGTTCACTTGGGGAAATGTTACTGTGAAATTGGCTCGTGCTTATGGCTTCTGCTATGGAGTTGAGCGAGCTGTGCAGATTGCTTTCGAAGCGAGGAAACAGTTTCCGAGTAGAACCATTTGGATTACCAATGAAATCATTCACAACCCCACTGTTAACAAGGTCCTCTGA

mRNA sequence

ATGGCGATTTCTCTGCAGATCCCCCGCGTTCCCCATCGCGCAGCCTTCATTTCACCCAGTCCCCTCTCCGCCGCCGGAGCTCTGCCTCTTCGCCTTCGGAAACCCTTTTCCCTCCGGTGCACCGCCGATTCTCCGTCTTCTCCGGGGCCGCTCGATCCCGATTTCGACGCGAAGTTGTTCTGGAAGAACTGGGCCAGAGGTGAAAACTACAACCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGAAGCTCATGAACAGCGAGTTCACGAGTGAAGTTATAAATACTCTGAATGAAAATGGGAACGAGTTCACTTGGGGAAATGTTACTGTGAAATTGGCTCGTGCTTATGGCTTCTGCTATGGAGTTGAGCGAGCTGTGCAGATTGCTTTCGAAGCGAGGAAACAGTTTCCGAGTAGAACCATTTGGATTACCAATGAAATCATTCACAACCCCACTGTTAACAAGGTCCTCTGA

Coding sequence (CDS)

ATGGCGATTTCTCTGCAGATCCCCCGCGTTCCCCATCGCGCAGCCTTCATTTCACCCAGTCCCCTCTCCGCCGCCGGAGCTCTGCCTCTTCGCCTTCGGAAACCCTTTTCCCTCCGGTGCACCGCCGATTCTCCGTCTTCTCCGGGGCCGCTCGATCCCGATTTCGACGCGAAGTTGTTCTGGAAGAACTGGGCCAGAGGTGAAAACTACAACCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGAAGCTCATGAACAGCGAGTTCACGAGTGAAGTTATAAATACTCTGAATGAAAATGGGAACGAGTTCACTTGGGGAAATGTTACTGTGAAATTGGCTCGTGCTTATGGCTTCTGCTATGGAGTTGAGCGAGCTGTGCAGATTGCTTTCGAAGCGAGGAAACAGTTTCCGAGTAGAACCATTTGGATTACCAATGAAATCATTCACAACCCCACTGTTAACAAGGTCCTCTGA

Protein sequence

MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL
Homology
BLAST of Moc02g10880 vs. NCBI nr
Match: XP_022148542.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 324.3 bits (830), Expect = 5.7e-85
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC
Sbjct: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNK L
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRL 158

BLAST of Moc02g10880 vs. NCBI nr
Match: XP_038902066.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X3 [Benincasa hispida])

HSP 1 Score: 255.0 bits (650), Expect = 4.3e-64
Identity = 125/158 (79.11%), Postives = 136/158 (86.08%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR P R  F+S    S  G   LR RKP  LRCTADS SSP P+DPDFDAKLF
Sbjct: 1   MAISLQIPRFPLRIDFVSRKTFS--GVRALRCRKPLFLRCTADSSSSPEPIDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSE++NTLNENG +FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGHKEETVKLMSSEFTSEIVNTLNENGKQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. NCBI nr
Match: XP_038902065.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 255.0 bits (650), Expect = 4.3e-64
Identity = 125/158 (79.11%), Postives = 136/158 (86.08%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR P R  F+S    S  G   LR RKP  LRCTADS SSP P+DPDFDAKLF
Sbjct: 1   MAISLQIPRFPLRIDFVSRKTFS--GVRALRCRKPLFLRCTADSSSSPEPIDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSE++NTLNENG +FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGHKEETVKLMSSEFTSEIVNTLNENGKQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. NCBI nr
Match: XP_038902064.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 255.0 bits (650), Expect = 4.3e-64
Identity = 125/158 (79.11%), Postives = 136/158 (86.08%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR P R  F+S    S  G   LR RKP  LRCTADS SSP P+DPDFDAKLF
Sbjct: 1   MAISLQIPRFPLRIDFVSRKTFS--GVRALRCRKPLFLRCTADSSSSPEPIDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSE++NTLNENG +FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGHKEETVKLMSSEFTSEIVNTLNENGKQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. NCBI nr
Match: XP_004145400.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus] >KAE8651867.1 hypothetical protein Csa_006064 [Cucumis sativus])

HSP 1 Score: 248.1 bits (632), Expect = 5.2e-62
Identity = 122/158 (77.22%), Postives = 132/158 (83.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ P  PHR  F SP   S  GA  LR RKP  LRCTADS SSP P+DPDFD KLF
Sbjct: 1   MAISLQSPCFPHRIQFFSPKTFSGVGA--LRCRKPLLLRCTADSSSSPKPVDPDFDPKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++NTLNENG +FTWG VTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETIKLMNSEFTSEIVNTLNENGYQFTWGKVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.2e-48
Identity = 97/162 (59.88%), Postives = 124/162 (76.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRA-AFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGP---LDPDFD 60
           MA++LQ  R+  R   F+  + LS +G+  LR RK  S+RC++   ++P P   +D DFD
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGS--LRRRKALSVRCSSGDENAPSPSVVMDSDFD 60

Query: 61  AKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARA 120
           AK+F KN  R +NYNRKGFGHKEET+KLMN E+TS+++ TL  NG  ++WG+VTVKLA+A
Sbjct: 61  AKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKA 120

Query: 121 YGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           YGFC+GVERAVQIA+EARKQFP   +WITNEIIHNPTVNK L
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRL 160

BLAST of Moc02g10880 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 6.7e-44
Identity = 93/149 (62.42%), Postives = 108/149 (72.48%), Query Frame = 0

Query: 13  RAAFISPSPLSAAGALPLRLRKPFSLRC---TADSPSSPGPLDPDFDAKLFWKNWARGEN 72
           R+A +SP    AA       R P S+RC    A S S+   LD DFD K F  N  R +N
Sbjct: 9   RSALLSP----AASPSRRARRAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDN 68

Query: 73  YNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFCYGVERAVQI 132
           YNRKGFGHK+ET++LM+ E+TS+VI TL ENGN+ TWG VTVKLA AYGFC+GVERAVQI
Sbjct: 69  YNRKGFGHKKETLELMSQEYTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQI 128

Query: 133 AFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           A+EARKQFP   IW+TNEIIHNPTVNK L
Sbjct: 129 AYEARKQFPDDRIWLTNEIIHNPTVNKRL 153

BLAST of Moc02g10880 vs. ExPASy Swiss-Prot
Match: B0C4N8 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=ispH PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-27
Identity = 55/103 (53.40%), Postives = 74/103 (71.84%), Query Frame = 0

Query: 56  DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
           D K F ++  + E Y+RKGFGH+EE   L+ SE+ S++I  +  NG     G+VT+ LA 
Sbjct: 2   DTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLAE 61

Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           A+GFC+GVERA+ +A+E R+QFP   IWITNEIIHNP+VNK L
Sbjct: 62  AFGFCWGVERAIALAYETRRQFPQERIWITNEIIHNPSVNKNL 104

BLAST of Moc02g10880 vs. ExPASy Swiss-Prot
Match: B0JVA7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=ispH PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.3e-27
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 0

Query: 56  DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
           D K F +   + +NYNRKGFGHKEE M  M +E+ S++I  + EN      G+VT+ LA+
Sbjct: 2   DTKSFKRTLQQSDNYNRKGFGHKEEVMDAMTNEYQSDLIQEIRENNYRLQRGDVTIYLAQ 61

Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           A+GFC+GVERAV +A+E R+ FP   IWITNEIIHNP+VN+ L
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQERIWITNEIIHNPSVNQRL 104

BLAST of Moc02g10880 vs. ExPASy Swiss-Prot
Match: B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 7.4e-27
Identity = 55/103 (53.40%), Postives = 75/103 (72.82%), Query Frame = 0

Query: 56  DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
           D K F ++  +  NY+RKGFGH+ E M  MN+E+ S +I  + EN  ++  G+VT+KLA 
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           A+GFC+GVERAV +A+E R+ FP   IWITNEIIHNP+VN+ L
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRL 104

BLAST of Moc02g10880 vs. ExPASy TrEMBL
Match: A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.8e-85
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC
Sbjct: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNK L
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRL 158

BLAST of Moc02g10880 vs. ExPASy TrEMBL
Match: A0A0A0LIX0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238840 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.5e-62
Identity = 122/158 (77.22%), Postives = 132/158 (83.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ P  PHR  F SP   S  GA  LR RKP  LRCTADS SSP P+DPDFD KLF
Sbjct: 1   MAISLQSPCFPHRIQFFSPKTFSGVGA--LRCRKPLLLRCTADSSSSPKPVDPDFDPKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++NTLNENG +FTWG VTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETIKLMNSEFTSEIVNTLNENGYQFTWGKVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. ExPASy TrEMBL
Match: A0A1S3CAB0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 6.2e-61
Identity = 120/158 (75.95%), Postives = 132/158 (83.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ PR PHR  F  P   S  GA  LR R+P  LRCTADS SSP P+DPDF+AKLF
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGA--LRCRQPLLLRCTADSSSSPKPVDPDFNAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++ TL ENG +FTWGNVTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. ExPASy TrEMBL
Match: A0A1S3CAZ8 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 6.2e-61
Identity = 120/158 (75.95%), Postives = 132/158 (83.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ PR PHR  F  P   S  GA  LR R+P  LRCTADS SSP P+DPDF+AKLF
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGA--LRCRQPLLLRCTADSSSSPKPVDPDFNAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++ TL ENG +FTWGNVTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. ExPASy TrEMBL
Match: A0A5A7TX23 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005030 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.1e-60
Identity = 120/158 (75.95%), Postives = 132/158 (83.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ PR PHR  F  P   S  GA  LR R+P  LRCTADS SSP P+DPDF+AKL 
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGA--LRCRQPLLLRCTADSSSSPKPVDPDFNAKLS 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++ TLNENG +FTWGNVTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLNENGFQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNK L
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRL 156

BLAST of Moc02g10880 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 194.1 bits (492), Expect = 8.3e-50
Identity = 97/162 (59.88%), Postives = 124/162 (76.54%), Query Frame = 0

Query: 1   MAISLQIPRVPHRA-AFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGP---LDPDFD 60
           MA++LQ  R+  R   F+  + LS +G+  LR RK  S+RC++   ++P P   +D DFD
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGS--LRRRKALSVRCSSGDENAPSPSVVMDSDFD 60

Query: 61  AKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARA 120
           AK+F KN  R +NYNRKGFGHKEET+KLMN E+TS+++ TL  NG  ++WG+VTVKLA+A
Sbjct: 61  AKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKA 120

Query: 121 YGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKVL 159
           YGFC+GVERAVQIA+EARKQFP   +WITNEIIHNPTVNK L
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRL 160

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148542.15.7e-8599.374-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Momordic... [more]
XP_038902066.14.3e-6479.114-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
XP_038902065.14.3e-6479.114-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
XP_038902064.14.3e-6479.114-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
XP_004145400.15.2e-6277.224-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativ... [more]
Match NameE-valueIdentityDescription
Q94B351.2e-4859.884-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
Q6AVG66.7e-4462.424-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
B0C4N82.0e-2753.404-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (stra... [more]
B0JVA73.3e-2754.374-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (st... [more]
B1WTZ27.4e-2753.404-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s... [more]
Match NameE-valueIdentityDescription
A0A6J1D5C22.8e-8599.374-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord... [more]
A0A0A0LIX02.5e-6277.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238840 PE=4 SV=1[more]
A0A1S3CAB06.2e-6175.954-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A1S3CAZ86.2e-6175.954-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A5A7TX231.1e-6075.954-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT4G34350.18.3e-5059.884-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 111..157
e-value: 1.1E-11
score: 44.5
NoneNo IPR availableGENE3D3.40.50.11270coord: 120..158
e-value: 8.3E-9
score: 37.4
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 30..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g10880.1Moc02g10880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0046429 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding