Moc02g10610 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g10610
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectinesterase
Location	chr2: 7530437 .. 7533042 (-)
RNA-Seq Expression	Moc02g10610
Synteny	Moc02g10610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAGGGGTCCTGCTCCTGCAGACATGAACACAGCACGGCTGATAAGAGTGGATCAGTCAGGAAGGGGGGATTTCAAGAAGATTCAAGACGCCATTGATTCTGTTCCTTCCCATAACAATGAACTTGTTTTCATTTGGGTTAAGCCTGGAACTTATAGGTTAAATTTCTCATCTCTATTCCATTAGTCTCAAATTTTGTCGTTAATCTCAACATTAGCTAATATTTTGAATAAAAAGTTGACATATGTATATAGAATTTGGGACTGCGTGGTAATTACCACGTTCCAATCTTACCTAAAACCTTCTCTTTCTATTGTTGAGATTAGGGTTGTGCTTTTCTATTGTCTATCCGATCTTGCCTTAATCTCTCCGTTAGAAATGATAGTTTCTCCCACATTTGTTATGTTTTCCGACTTAATACAAGTAAATTTCCAGGTTAATTACCTTATGACAGTTCTAAATTCTATCATATATCAAATTTCTATTCAAGATTTTAATGAATATTTTTTTAAAGGGTATTTTTGAAACATTTAGAAAACAAAGGAAAATACTTTACTCTGAACAATTTTATCCTCGTTCATATTAAATGTTTGTCAATTTTTGTAATGATGGCTGAGGACAAAGATTACACAACTGTACGAAGCTAATTATTTTCCCATGCAAATTAGCCTTTTTGTTTCTCCTATTTATATGTAATTAATTTTGTTTGTTTTCGAACGTTTTAAGATTAAATCGAAACTTTTTACTTTAAATTAAAGAAGCTCTTCTAAATTCCAGTTGAGAATTCTTAGAGTGTTTTTTAATCTTAATTAAATAAAATCCAGATATTAAACAATTATGTTATGTCTCTGTGGTAATTTAATTGGGATTTTCCTTTTCTTTTTTCGAAATTGGGCTGCAGGGAAAAAATTGTGGTGCCGGAGGAGAAGCCGTACATAACACTGAGCGGCAGCAACGCCGCCGACACCATAATAACGTGGAAGCAAGGGACAGATTTACTGGAATCTGCGGCCATCTCTGTGTTCGCTCCGGACTTCGTCGGCCGTTTTCTGACCATCCAGAACACGTTCGGCAGCAGCGGCATTGCGGTGGCGCTACGTGTCTCCGCTGACAGAGCCGCCTTCTACGGCTGCCGGATAATATCCTATCAAGACACGCTTCTCGACGACGCCGGAAGGCATTATTTCAAGAACTGCTACATCGAAGGAGCCACCGATTTCATCTGCGGAAACGCCGCTTCTCTCTATGAGGTTCTAGTTAATTGAATTCTAAAATTTCTGTTTCTTTTGAGATTTTTTTTGTTTATTTTCAATTCTTTGTTAAAATTTGTTTTATTTTGGTTAGTAAAAATGTCTAGTTTTATCCTTAAATTTTTAAAATATAATTATATCATTTTTTGAAAATTGGGTTTATTTAGTCACAATTTTTTTCCGTATATTTTTATTTTTTTAAAATCAAATTTCAATTCTTAGCTAAATTTAAAAAAGAGAAACAAGCTCTTAAAACTATTTTTTTAGTCTAACAAAATATTAAACAAAAAGATAATAAAACAAAAAATTCTGTGGTTGAAAATAGTGTTTTACCAACTTAATTTTCAAAAATTGAAAACCACGCGTAAAATTTATTATTGGAGCCTTAACTATTTAAATTTTAAGGTCAAAGCGGACATAGGTCAGTGGTTATCTCCAACCAAAAGGTTGTGAATTCAATCTCCTCCGCACATGTTTTACTAGAAAAAAAAAACTATTAAAAAACAAACGAATGTCTTCATGAAGAGCAGTTTAACTTGGCTTAATTTTTTAAAATAAAAAACTGAAAACAAAATAGTTATCAAACGAGTCTAACATTTTAGTTTATTGAGTTGTAGAAGTGTCACTTGCATTCAACGTCGGAGAGGGGTGGGGCAATGACTGCGCAACACCGAAACTCGGGGGAGGAGAACACTGGGTTCATGTTCTTGGGGTGTAAAATCACAGGAACTGGATCGACTTTGCTTGGACGGCCATGGGGGGCTTGCTCTAGGGTTGTGTTTGGCTACACTTACATGTCCAATGTTGTTGTTCCTAAAGGCTGGAGTGATTGGGGAGATCAAACCAAGCAAAGGTAATAAATGGCCTATCATTGTTTTAGGTTAAATTCTTGCATTTAAACTTCACAAATTATTTCCAATATGGTTTACAAGAACTAAAACCAACCTGAAAAATAGGCCAAAATAGTATTTTAGTATTTTACACTTGTTTTTAGTGAAATTACAAGTTTAGTTTTTGGACTTTTAAGGTTATGTTTAATAGGTATATAAAATTTCAGTTTTGTATCTAATAGATTTCTTTAATGGTGTCTAAATAGAGATCCCAATAACGGGTCTAAATTTAAATTTTATGATTTTGTGCGAGGTAATGAACAGAACGGTGGTATATGGAGAATACAAGTGCTACGGGGTAGGGGCCAACAGAGCCAAAAGAGTTTCGTGGTCGCGCAACCTATCGACCAATGAAGCTGCTGTCTTCTTCACTAAAGACATGATCGGCGGCCGAGCTTGGCTTCGACCTTCGCCTTCTCATTTCAAGAGGAGCTCTTCCACTTCTTCCACCATTAATGTCTAA

mRNA sequence

ATGGCAAGGGGTCCTGCTCCTGCAGACATGAACACAGCACGGCTGATAAGAGTGGATCAGTCAGGAAGGGGGGATTTCAAGAAGATTCAAGACGCCATTGATTCTGTTCCTTCCCATAACAATGAACTTGTTTTCATTTGGGTTAAGCCTGGAACTTATAGGGAAAAAATTGTGGTGCCGGAGGAGAAGCCGTACATAACACTGAGCGGCAGCAACGCCGCCGACACCATAATAACGTGGAAGCAAGGGACAGATTTACTGGAATCTGCGGCCATCTCTGTGTTCGCTCCGGACTTCGTCGGCCGTTTTCTGACCATCCAGAACACGTTCGGCAGCAGCGGCATTGCGGTGGCGCTACGTGTCTCCGCTGACAGAGCCGCCTTCTACGGCTGCCGGATAATATCCTATCAAGACACGCTTCTCGACGACGCCGGAAGGCATTATTTCAAGAACTGCTACATCGAAGGAGCCACCGATTTCATCTGCGGAAACGCCGCTTCTCTCTATGAGAAGTGTCACTTGCATTCAACGTCGGAGAGGGGTGGGGCAATGACTGCGCAACACCGAAACTCGGGGGAGGAGAACACTGGGTTCATGTTCTTGGGGTGTAAAATCACAGGAACTGGATCGACTTTGCTTGGACGGCCATGGGGGGCTTGCTCTAGGGTTGTGTTTGGCTACACTTACATGTCCAATGTTGTTGTTCCTAAAGGCTGGAGTGATTGGGGAGATCAAACCAAGCAAAGAACGGTGGTATATGGAGAATACAAGTGCTACGGGGTAGGGGCCAACAGAGCCAAAAGAGTTTCGTGGTCGCGCAACCTATCGACCAATGAAGCTGCTGTCTTCTTCACTAAAGACATGATCGGCGGCCGAGCTTGGCTTCGACCTTCGCCTTCTCATTTCAAGAGGAGCTCTTCCACTTCTTCCACCATTAATGTCTAA

Coding sequence (CDS)

ATGGCAAGGGGTCCTGCTCCTGCAGACATGAACACAGCACGGCTGATAAGAGTGGATCAGTCAGGAAGGGGGGATTTCAAGAAGATTCAAGACGCCATTGATTCTGTTCCTTCCCATAACAATGAACTTGTTTTCATTTGGGTTAAGCCTGGAACTTATAGGGAAAAAATTGTGGTGCCGGAGGAGAAGCCGTACATAACACTGAGCGGCAGCAACGCCGCCGACACCATAATAACGTGGAAGCAAGGGACAGATTTACTGGAATCTGCGGCCATCTCTGTGTTCGCTCCGGACTTCGTCGGCCGTTTTCTGACCATCCAGAACACGTTCGGCAGCAGCGGCATTGCGGTGGCGCTACGTGTCTCCGCTGACAGAGCCGCCTTCTACGGCTGCCGGATAATATCCTATCAAGACACGCTTCTCGACGACGCCGGAAGGCATTATTTCAAGAACTGCTACATCGAAGGAGCCACCGATTTCATCTGCGGAAACGCCGCTTCTCTCTATGAGAAGTGTCACTTGCATTCAACGTCGGAGAGGGGTGGGGCAATGACTGCGCAACACCGAAACTCGGGGGAGGAGAACACTGGGTTCATGTTCTTGGGGTGTAAAATCACAGGAACTGGATCGACTTTGCTTGGACGGCCATGGGGGGCTTGCTCTAGGGTTGTGTTTGGCTACACTTACATGTCCAATGTTGTTGTTCCTAAAGGCTGGAGTGATTGGGGAGATCAAACCAAGCAAAGAACGGTGGTATATGGAGAATACAAGTGCTACGGGGTAGGGGCCAACAGAGCCAAAAGAGTTTCGTGGTCGCGCAACCTATCGACCAATGAAGCTGCTGTCTTCTTCACTAAAGACATGATCGGCGGCCGAGCTTGGCTTCGACCTTCGCCTTCTCATTTCAAGAGGAGCTCTTCCACTTCTTCCACCATTAATGTCTAA

Protein sequence

MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKRSSSTSSTINV

Homology

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BLAST of Moc02g10610 vs. NCBI nr
Match: XP_022146302.1 (putative pectinesterase 11 [Momordica charantia])

HSP 1 Score: 648.7 bits (1672), Expect = 2.6e-182
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVP 60
           MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVP
Sbjct: 1   MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVP 60

Query: 61  EEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALR 120
           EEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALR
Sbjct: 61  EEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALR 120

Query: 121 VSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSER 180
           VSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSER
Sbjct: 121 VSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSER 180

Query: 181 GGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWS 240
           GGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWS
Sbjct: 181 GGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWS 240

Query: 241 DWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPS 300
           DWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPS
Sbjct: 241 DWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPS 300

Query: 301 HFKRSSSTSSTINV 315
           HFKRSSSTSSTINV
Sbjct: 301 HFKRSSSTSSTINV 314

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BLAST of Moc02g10610 vs. NCBI nr
Match: XP_022947689.1 (putative pectinesterase 11 isoform X2 [Cucurbita moschata])

HSP 1 Score: 557.0 bits (1434), Expect = 1.0e-154
Identity = 262/303 (86.47%), Postives = 285/303 (94.06%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPY 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVPS NNELVFIW+KPGTYREKIVVPE+KP+
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKPF 61

Query: 66  ITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSADR 125
           ITLSGS A+DTIITW QG DLL+S AIS+FA DFVGRFLTIQNT+G++GIAVALRVSADR
Sbjct: 62  ITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSADR 121

Query: 126 AAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 185
           AAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT
Sbjct: 122 AAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 181

Query: 186 AQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGDQ 245
           AQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD 
Sbjct: 182 AQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGDH 241

Query: 246 TKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKRS 305
           TKQRTV YGEYKCYG GANRAKRV WSR+LST+EAA  FTKDMIGGR WLRP+PSHFKRS
Sbjct: 242 TKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKRS 301

Query: 306 SST 309
           SST
Sbjct: 302 SST 304

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BLAST of Moc02g10610 vs. NCBI nr
Match: KAG6605075.1 (putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 555.4 bits (1430), Expect = 3.0e-154
Identity = 260/302 (86.09%), Postives = 284/302 (94.04%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPY 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVPS NNELVFIW+KPGTYREKIVVPE+KP+
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKPF 61

Query: 66  ITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSADR 125
           ITLSGS A+DTIITW QG DLL+S AIS+FA DFVGRFLTIQNT+G++GIAVALRVSADR
Sbjct: 62  ITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSADR 121

Query: 126 AAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 185
           AAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT
Sbjct: 122 AAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 181

Query: 186 AQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGDQ 245
           AQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD 
Sbjct: 182 AQHRNTAAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGDH 241

Query: 246 TKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKRS 305
           TKQRTV YGEYKCYG GANRAKRV+WSR+LS +EAA  FTKDMIGGR WLRP+PSHFKRS
Sbjct: 242 TKQRTVFYGEYKCYGPGANRAKRVAWSRSLSMDEAAKLFTKDMIGGRGWLRPAPSHFKRS 301

Query: 306 SS 308
           SS
Sbjct: 302 SS 303

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BLAST of Moc02g10610 vs. NCBI nr
Match: XP_023533155.1 (putative pectinesterase 11 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023533156.1 putative pectinesterase 11 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553.1 bits (1424), Expect = 1.5e-153
Identity = 258/303 (85.15%), Postives = 284/303 (93.73%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPY 65
           AP DM+TARLIRVDQSG+GDF+KIQ+AIDSVPS NNELVFIW+KPGTYREKIVVPE+KP+
Sbjct: 2   APVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKPF 61

Query: 66  ITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSADR 125
           ITLSGS A+DTIITW QG DLL+S AIS+FA DFVGRFLTIQNT+G++GIAVALRVSADR
Sbjct: 62  ITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSADR 121

Query: 126 AAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 185
           AAFYGCRIIS+QDTLLDD G+HYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT
Sbjct: 122 AAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 181

Query: 186 AQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGDQ 245
           AQHRN+  ENTGF+FLGCKITG+GST LGRPWG CSRVVFGYT+MSNV+VP+GW+DWGD 
Sbjct: 182 AQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHMSNVIVPEGWNDWGDH 241

Query: 246 TKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKRS 305
           TKQRTV YGEYKCYG GANRAKRV+WSR+LS +EAA  FTKDMIGGR WLRP+PSHFKRS
Sbjct: 242 TKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDMIGGRGWLRPAPSHFKRS 301

Query: 306 SST 309
           SST
Sbjct: 302 SST 304

[Close]

BLAST of Moc02g10610 vs. NCBI nr
Match: XP_022947688.1 (putative pectinesterase 11 isoform X1 [Cucurbita moschata])

HSP 1 Score: 552.4 bits (1422), Expect = 2.6e-153
Identity = 262/304 (86.18%), Postives = 285/304 (93.75%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTY-REKIVVPEEKP 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVPS NNELVFIW+KPGTY REKIVVPE+KP
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSREKIVVPEDKP 61

Query: 66  YITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSAD 125
           +ITLSGS A+DTIITW QG DLL+S AIS+FA DFVGRFLTIQNT+G++GIAVALRVSAD
Sbjct: 62  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 121

Query: 126 RAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 185
           RAAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM
Sbjct: 122 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 181

Query: 186 TAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGD 245
           TAQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD
Sbjct: 182 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 241

Query: 246 QTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKR 305
            TKQRTV YGEYKCYG GANRAKRV WSR+LST+EAA  FTKDMIGGR WLRP+PSHFKR
Sbjct: 242 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKR 301

Query: 306 SSST 309
           SSST
Sbjct: 302 SSST 305

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BLAST of Moc02g10610 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-123
Identity = 203/303 (67.00%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 5   PAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPS--HNNELVFIWVKPGTYREKIVVPEE 64
           P+  D +TA LIRVDQSG+GDF KIQ+AI+S+P   +N++L FIWVKPG YREK+V+P E
Sbjct: 40  PSSIDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAE 99

Query: 65  KPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVS 124
           KPYITLSG+ A++T + W  G D+LES  +++FA DFV RFLTIQN FG++G AVALRV+
Sbjct: 100 KPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVA 159

Query: 125 ADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGG 184
           AD+AAFYGC I SYQDTLLDD G HYFKNCYIEGATDFICG+A+SLYE+CHLHS S   G
Sbjct: 160 ADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNG 219

Query: 185 AMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDW 244
           ++TAQ R S  E +GF FLGCK+TG+GST LGRPWGA SRVVF Y++ SNVV P+GW+ W
Sbjct: 220 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 279

Query: 245 GDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHF 304
           GD TK+ TV YGEYKCYG GA+R +RV WS+ LS  EA VF +KD IGG+ WLRP+PSHF
Sbjct: 280 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHF 339

Query: 305 KRS 306
           K +
Sbjct: 340 KNA 342

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BLAST of Moc02g10610 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.1e-77
Identity = 145/293 (49.49%), Postives = 194/293 (66.21%), Query Frame = 0


Query: 20  QSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGSNAADTIIT 79
           +S +GDF KIQDAIDS+P  N   V I V  G Y+EK+ +P  K +IT+ G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 80  W--------KQGTDL--LESAAISVFAPDFVGRFLTIQNTF-----GSSG-IAVALRVSA 139
           W         +G  +    SA+ +V +P FV + +T +NT      G+ G  AVALRVSA
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 140 DRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGA 199
           D AAF+GCR++  QDTL D  GRHY+K+CYIEG+ DFI GNA SLYE CH+H+ +++ GA
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 200 MTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWG 259
           +TAQ R+S  E+TGF F+ CK+TGTG   LGR WG  SRVVF YTYM N+++P+GW +WG
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 260 DQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLR 297
           D +++ TV YG+YKC G GAN   RV+W+R L+  EA  F +   I G  W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of Moc02g10610 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.6e-71
Identity = 136/303 (44.88%), Postives = 189/303 (62.38%), Query Frame = 0


Query: 11  NTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSG 70
           N  R+I VD++G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KPYI+  G
Sbjct: 77  NVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIG 136

Query: 71  --SNAADTIITWK-QGTDL---------LESAAISVFAPDFVGRFLTIQNTF----GSSG 130
             S A DT+I+W  + +DL           +A++S+ +  F    +T +NT     G  G
Sbjct: 137 NESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQG 196

Query: 131 -IAVALRVSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCH 190
             AVALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C 
Sbjct: 197 RQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCD 256

Query: 191 LHSTSERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNV 250
           +HST++R GA+ A HR+S  E+TGF F+ C I+GTG   LGR WG  SR V+   +++++
Sbjct: 257 IHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADI 316

Query: 251 VVPKGWSDWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRA 297
           + P GWSDW    +QR V++GEY C G GA R  RV WS+ L+ +E   F  ++ I G  
Sbjct: 317 ITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQ 376

[Close]

BLAST of Moc02g10610 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.0e-69
Identity = 132/301 (43.85%), Postives = 178/301 (59.14%), Query Frame = 0


Query: 14  RLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGSNA 73
           ++I V  +G   F+ +QDA+DS+P +NN+ + I + PG YREK+VVP  KPYIT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 74  ADTIITW-KQGTDL---------LESAAISVFAPDFVGRFLTIQNTF-----GSSG-IAV 133
             T I W  + +DL          ++A+++V+A  F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 134 ALRVSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHST 193
           A R+S D+A F GC     QDTL DDAGRHYFK CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 194 SERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPK 253
           + R G++ A  R   EE TGF F+GC++TGTG   +GR  G  SR+V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 254 GWSDWGDQT-KQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLR 298
           GW DW  ++ K +T  +G Y CYG GA   + VSW+R L    A  F  K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

[Close]

BLAST of Moc02g10610 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.8e-67
Identity = 130/300 (43.33%), Postives = 182/300 (60.67%), Query Frame = 0


Query: 12  TARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGS 71
           T+ ++ VD  G G+F  +Q AID VP  ++    I V  G+YREK+ V E K  + + G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 72  NAADTIITW----KQGTDLLESAAISVFAPDFVGRFLTIQNTF------GSSGIAVALRV 131
              +T I W    K   +  +S +  VFA +F    ++ +N         +   AVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 132 SADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHS----- 191
             D+AAFYGC     QDTLLDD GRH+FK C+I+G+ DFI GN  SLY+ C ++S     
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 192 TSERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVP 251
           TS   G++TAQ R S +E +GF F+ CKI G+G  LLGR WGA + VVF  TYMS ++ P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 252 KGWSDWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLR 297
           +GW++WGD TK++TV +GE+KCYG GA+  +RV + + L+ +EA+ F     I G  WLR
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of Moc02g10610 vs. ExPASy TrEMBL
Match: A0A6J1CZ83 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015541 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.3e-182
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVP 60
           MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVP
Sbjct: 1   MARGPAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVP 60

Query: 61  EEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALR 120
           EEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALR
Sbjct: 61  EEKPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALR 120

Query: 121 VSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSER 180
           VSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSER
Sbjct: 121 VSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSER 180

Query: 181 GGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWS 240
           GGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWS
Sbjct: 181 GGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWS 240

Query: 241 DWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPS 300
           DWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPS
Sbjct: 241 DWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPS 300

Query: 301 HFKRSSSTSSTINV 315
           HFKRSSSTSSTINV
Sbjct: 301 HFKRSSSTSSTINV 314

[Close]

BLAST of Moc02g10610 vs. ExPASy TrEMBL
Match: A0A6J1G7K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.1e-155
Identity = 262/303 (86.47%), Postives = 285/303 (94.06%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPY 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVPS NNELVFIW+KPGTYREKIVVPE+KP+
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKPF 61

Query: 66  ITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSADR 125
           ITLSGS A+DTIITW QG DLL+S AIS+FA DFVGRFLTIQNT+G++GIAVALRVSADR
Sbjct: 62  ITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSADR 121

Query: 126 AAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 185
           AAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT
Sbjct: 122 AAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 181

Query: 186 AQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGDQ 245
           AQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD 
Sbjct: 182 AQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGDH 241

Query: 246 TKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKRS 305
           TKQRTV YGEYKCYG GANRAKRV WSR+LST+EAA  FTKDMIGGR WLRP+PSHFKRS
Sbjct: 242 TKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKRS 301

Query: 306 SST 309
           SST
Sbjct: 302 SST 304

[Close]

BLAST of Moc02g10610 vs. ExPASy TrEMBL
Match: A0A6J1G7A7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.2e-153
Identity = 262/304 (86.18%), Postives = 285/304 (93.75%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTY-REKIVVPEEKP 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVPS NNELVFIW+KPGTY REKIVVPE+KP
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSREKIVVPEDKP 61

Query: 66  YITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSAD 125
           +ITLSGS A+DTIITW QG DLL+S AIS+FA DFVGRFLTIQNT+G++GIAVALRVSAD
Sbjct: 62  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 121

Query: 126 RAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 185
           RAAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM
Sbjct: 122 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 181

Query: 186 TAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGD 245
           TAQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD
Sbjct: 182 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 241

Query: 246 QTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKR 305
            TKQRTV YGEYKCYG GANRAKRV WSR+LST+EAA  FTKDMIGGR WLRP+PSHFKR
Sbjct: 242 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKR 301

Query: 306 SSST 309
           SSST
Sbjct: 302 SSST 305

[Close]

BLAST of Moc02g10610 vs. ExPASy TrEMBL
Match: A0A6J1KXA2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.8e-153
Identity = 259/303 (85.48%), Postives = 283/303 (93.40%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPY 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVP  NNELVFIW+KPGTYREKIVVPE+KP+
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPPRNNELVFIWIKPGTYREKIVVPEDKPF 61

Query: 66  ITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSADR 125
           ITLSGS A+DTIITW QG DLL+S AISVFA DFV RFLTIQNT+G++GIAVALRVSADR
Sbjct: 62  ITLSGSKASDTIITWNQGRDLLQSPAISVFASDFVARFLTIQNTYGTTGIAVALRVSADR 121

Query: 126 AAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAMT 185
           AAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHS SERGGAMT
Sbjct: 122 AAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSMSERGGAMT 181

Query: 186 AQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGDQ 245
           AQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD 
Sbjct: 182 AQHRNTTAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGDH 241

Query: 246 TKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKRS 305
           TKQRTV YGEYKCYG G+NRAKRV+WSR+LST+EAA  FTKDMIGGR WLRP+PSHFKRS
Sbjct: 242 TKQRTVFYGEYKCYGPGSNRAKRVAWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKRS 301

Query: 306 SST 309
           SST
Sbjct: 302 SST 304

[Close]

BLAST of Moc02g10610 vs. ExPASy TrEMBL
Match: A0A6J1KZA2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.8e-152
Identity = 259/304 (85.20%), Postives = 283/304 (93.09%), Query Frame = 0


Query: 6   APADMNTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTY-REKIVVPEEKP 65
           APADM+TARLIRVDQSG+GDF+KIQ+AIDSVP  NNELVFIW+KPGTY REKIVVPE+KP
Sbjct: 2   APADMSTARLIRVDQSGKGDFRKIQEAIDSVPPRNNELVFIWIKPGTYSREKIVVPEDKP 61

Query: 66  YITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVSAD 125
           +ITLSGS A+DTIITW QG DLL+S AISVFA DFV RFLTIQNT+G++GIAVALRVSAD
Sbjct: 62  FITLSGSKASDTIITWNQGRDLLQSPAISVFASDFVARFLTIQNTYGTTGIAVALRVSAD 121

Query: 126 RAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 185
           RAAFYGCRIIS+QDTLLDD GRHYFKNCYIEGATDFICGNAASLYEKCHLHS SERGGAM
Sbjct: 122 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSMSERGGAM 181

Query: 186 TAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWGD 245
           TAQHRN+  ENTGF+FLGCKITG+GS LLGRPWG CSRVVFGYT+MSNVVVP+GW+DWGD
Sbjct: 182 TAQHRNTTAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 241

Query: 246 QTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHFKR 305
            TKQRTV YGEYKCYG G+NRAKRV+WSR+LST+EAA  FTKDMIGGR WLRP+PSHFKR
Sbjct: 242 HTKQRTVFYGEYKCYGPGSNRAKRVAWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKR 301

Query: 306 SSST 309
           SSST
Sbjct: 302 SSST 305

[Close]

BLAST of Moc02g10610 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 203/303 (67.00%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 5   PAPADMNTARLIRVDQSGRGDFKKIQDAIDSVPS--HNNELVFIWVKPGTYREKIVVPEE 64
           P+  D +TA LIRVDQSG+GDF KIQ+AI+S+P   +N++L FIWVKPG YREK+V+P E
Sbjct: 40  PSSIDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAE 99

Query: 65  KPYITLSGSNAADTIITWKQGTDLLESAAISVFAPDFVGRFLTIQNTFGSSGIAVALRVS 124
           KPYITLSG+ A++T + W  G D+LES  +++FA DFV RFLTIQN FG++G AVALRV+
Sbjct: 100 KPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVA 159

Query: 125 ADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGG 184
           AD+AAFYGC I SYQDTLLDD G HYFKNCYIEGATDFICG+A+SLYE+CHLHS S   G
Sbjct: 160 ADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNG 219

Query: 185 AMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDW 244
           ++TAQ R S  E +GF FLGCK+TG+GST LGRPWGA SRVVF Y++ SNVV P+GW+ W
Sbjct: 220 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 279

Query: 245 GDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLRPSPSHF 304
           GD TK+ TV YGEYKCYG GA+R +RV WS+ LS  EA VF +KD IGG+ WLRP+PSHF
Sbjct: 280 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHF 339

Query: 305 KRS 306
           K +
Sbjct: 340 KNA 342

[Close]

BLAST of Moc02g10610 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 2.2e-78
Identity = 145/293 (49.49%), Postives = 194/293 (66.21%), Query Frame = 0


Query: 20  QSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGSNAADTIIT 79
           +S +GDF KIQDAIDS+P  N   V I V  G Y+EK+ +P  K +IT+ G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 80  W--------KQGTDL--LESAAISVFAPDFVGRFLTIQNTF-----GSSG-IAVALRVSA 139
           W         +G  +    SA+ +V +P FV + +T +NT      G+ G  AVALRVSA
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 140 DRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGA 199
           D AAF+GCR++  QDTL D  GRHY+K+CYIEG+ DFI GNA SLYE CH+H+ +++ GA
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 200 MTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPKGWSDWG 259
           +TAQ R+S  E+TGF F+ CK+TGTG   LGR WG  SRVVF YTYM N+++P+GW +WG
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 260 DQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLR 297
           D +++ TV YG+YKC G GAN   RV+W+R L+  EA  F +   I G  W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of Moc02g10610 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 1.8e-72
Identity = 136/303 (44.88%), Postives = 189/303 (62.38%), Query Frame = 0


Query: 11  NTARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSG 70
           N  R+I VD++G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KPYI+  G
Sbjct: 77  NVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIG 136

Query: 71  --SNAADTIITWK-QGTDL---------LESAAISVFAPDFVGRFLTIQNTF----GSSG 130
             S A DT+I+W  + +DL           +A++S+ +  F    +T +NT     G  G
Sbjct: 137 NESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQG 196

Query: 131 -IAVALRVSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCH 190
             AVALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C 
Sbjct: 197 RQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCD 256

Query: 191 LHSTSERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNV 250
           +HST++R GA+ A HR+S  E+TGF F+ C I+GTG   LGR WG  SR V+   +++++
Sbjct: 257 IHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADI 316

Query: 251 VVPKGWSDWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRA 297
           + P GWSDW    +QR V++GEY C G GA R  RV WS+ L+ +E   F  ++ I G  
Sbjct: 317 ITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQ 376

[Close]

BLAST of Moc02g10610 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.0e-70
Identity = 132/301 (43.85%), Postives = 178/301 (59.14%), Query Frame = 0


Query: 14  RLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGSNA 73
           ++I V  +G   F+ +QDA+DS+P +NN+ + I + PG YREK+VVP  KPYIT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 74  ADTIITW-KQGTDL---------LESAAISVFAPDFVGRFLTIQNTF-----GSSG-IAV 133
             T I W  + +DL          ++A+++V+A  F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 134 ALRVSADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHST 193
           A R+S D+A F GC     QDTL DDAGRHYFK CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 194 SERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVPK 253
           + R G++ A  R   EE TGF F+GC++TGTG   +GR  G  SR+V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 254 GWSDWGDQT-KQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLR 298
           GW DW  ++ K +T  +G Y CYG GA   + VSW+R L    A  F  K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

[Close]

BLAST of Moc02g10610 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 2.7e-68
Identity = 130/300 (43.33%), Postives = 182/300 (60.67%), Query Frame = 0


Query: 12  TARLIRVDQSGRGDFKKIQDAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPYITLSGS 71
           T+ ++ VD  G G+F  +Q AID VP  ++    I V  G+YREK+ V E K  + + G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 72  NAADTIITW----KQGTDLLESAAISVFAPDFVGRFLTIQNTF------GSSGIAVALRV 131
              +T I W    K   +  +S +  VFA +F    ++ +N         +   AVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 132 SADRAAFYGCRIISYQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYEKCHLHS----- 191
             D+AAFYGC     QDTLLDD GRH+FK C+I+G+ DFI GN  SLY+ C ++S     
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 192 TSERGGAMTAQHRNSGEENTGFMFLGCKITGTGSTLLGRPWGACSRVVFGYTYMSNVVVP 251
           TS   G++TAQ R S +E +GF F+ CKI G+G  LLGR WGA + VVF  TYMS ++ P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 252 KGWSDWGDQTKQRTVVYGEYKCYGVGANRAKRVSWSRNLSTNEAAVFFTKDMIGGRAWLR 297
           +GW++WGD TK++TV +GE+KCYG GA+  +RV + + L+ +EA+ F     I G  WLR
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

The following BLAST results are available for this feature:

BLAST of Moc02g10610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146302.1	2.6e-182	100.00	putative pectinesterase 11 [Momordica charantia]	[more]
XP_022947689.1	1.0e-154	86.47	putative pectinesterase 11 isoform X2 [Cucurbita moschata]	[more]
KAG6605075.1	3.0e-154	86.09	putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023533155.1	1.5e-153	85.15	putative pectinesterase 11 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023533156...	[more]
XP_022947688.1	2.6e-153	86.18	putative pectinesterase 11 isoform X1 [Cucurbita moschata]	[more]

BLAST of Moc02g10610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIJ9	1.7e-123	67.00	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8VYZ3	3.1e-77	49.49	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9FM79	2.6e-71	44.88	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q8LPF3	7.0e-69	43.85	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9ZQA3	3.8e-67	43.33	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of Moc02g10610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZ83	1.3e-182	100.00	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015541 PE=4 SV=1	[more]
A0A6J1G7K1	5.1e-155	86.47	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1	[more]
A0A6J1G7A7	1.2e-153	86.18	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1	[more]
A0A6J1KXA2	2.8e-153	85.48	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1	[more]
A0A6J1KZA2	6.8e-152	85.20	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1	[more]

BLAST of Moc02g10610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21610.1	1.2e-124	67.00	pectinesterase 11	[more]
AT5G19730.1	2.2e-78	49.49	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	1.8e-72	44.88	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	5.0e-70	43.85	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	2.7e-68	43.33	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 18..290 e-value: 4.2E-68 score: 229.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 10..305 e-value: 2.8E-105 score: 354.2
None	No IPR available	PANTHER	PTHR31321:SF77	PECTINESTERASE 11-RELATED	coord: 10..296
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 10..296
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 12..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g10610.1	Moc02g10610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity