Moc02g09330 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g09330
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptiontRNA-intron lyase
Locationchr2: 6618905 .. 6624807 (-)
RNA-Seq ExpressionMoc02g09330
SyntenyMoc02g09330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCCAAGATGGAAAGGGAAGGGTTCAGAAGCCAAAGCTCTAGCAGATCCCATGTCGAAGATTGTACTTCAGCTTCAATCTTCTCTAACACAATCGGACGCTCAGGGACTATTGTCCGGATGTAGTGTACTTTATCAAGCAGATGAAGAACAAACCGATCTTTTCAATCGCGCTTGTTTCGGTCGACCCATTGTTACAGCCGATAAGGATAAGCAATGGTTTCAATTGGGCGTGGAGGAGGCGTTTTACTTGTCCTGTCATTTGAAATGCCTCAAAATTGTCGATGGACAAGATTGTGAAAAGAATGCTGAACAGCTGTGGGAGTATATGAAGTCCCAAAAGGCTACGTTCCCTGAGTTTTACAAGGCTTATCAACATCTCCGGATGAAGAATTGGGTTGTCAGACCTGGATCTCAATACGGGGTTGATTTTGTGGCTTATCGTCACCATCCGGCTCTCGTCCACTCTGAATTTGCTGTTCTTGTGTTGTCCGAAGGAGGAGGAGACAGTAACGAAAATGGGCGATTGAGAGTTTGGTCTGATTTTCTCTGCACAATTCGGCTCTGTGGAAGCGTTGCAAAAACGCTGCTAGTTCTTAGCGTCAATAACAATGGAAGCAGTTCTGTTTCTCCTTCATTTTTGGACAGCTACTCCATTGAAGAACGTACGGTTACAAGATGGAGTCCAGAACAGTGCCGTGAAAATCCTGCAAAAGTGTGAATTATACCAAGTAAGATATGATGTAACCTCTTTATTTGTTATTTGCCTAATTCCCGGTGGTTCTTCATGTATAATGAAAATTGAGTAGCTAGAGCCACTTAAGATGTTCAGTTTTAAATGGGTTTTGATTCTTTTGAAGATGATATAGTCACATCACAACACAATCATGTTCTTCCAATTAAACTTGAATATTTTTCGTGTAGGAAATTGTTGAAGTATCCATACTTTCAGGAATAAAAACAACCCAACTGAACATTGTTGAATGTAAAGATCCAATCCCCACAATACATACACAAACAATCACTCATTCGAGATGAAAGATTAGTTGATGTTAGATTACCAGAAACCTCAAAATTTAAATTAAAAAGCTCAATAGCTGTTGGGTTGAAAATTAGCAAAATACTTAACAAGTGAAATATTTGAGGAAATGACAAGAGGTTGAACATAGAATTTTATGGTTTGATACATGTTAAACTACTACTAAACTCAAAATAATATGTTAAAAGTATAATTTAATATTTTGTTATACTCTCTGCCATTTAAAAAATATCTTCTGGGTTTTGCCTTCTCCATCCATACTTTCCTCCTTGGGGGGTTAGTCTGTTTTGATCATTAGTTGCTCCTGAATTGTCCAATGGAAATGTCATAAGCAAACAAAAGAAAATGGTTTTAATGTCAGAGACATGTTCGTCTGGCTCCTTTTGAGGCTGGCCACTGCACCTTCTATGCAATAGTGGTAGACATACAGAGTTCGTCTTGATAATTGCTCCACTTTCTGCACTTTGGATTGGCAGTTAGCCAATTCAAAATGTCTCTTTGTACTACGTCAAAGACTGAACAAACCAAATTATTGGAAAAAAGTTCGTATTTCAAGTATTGTATCACAACAAGTAATCACGTTTTGTACACAATAAAGCCACTACGCTAAATTATTTCAAAAAAAAAAAAAAAATTGACTTTCAAGTACTGTTGCTTTCTTTTGCATATGCTTATACAGTGTACTTGATTTAATGACTAGAAAAGGGAACATCCTAATTTCTGAAGTAGGCCCTCATGCACTTGTTAATGCTTTATAGTTTATATGCAGTCAAAGGTGGTCTAACGAAGAAGAAGAATCGCGTGGGGAAATTGAAACTATGAATTTTGAAAAAGAAGGTAGATAGGAAAAGGCAGAGGCTTAGTCAAACATATCGATTGCTCGAGCACCATGAAGTACCTAGGGAAATCGGAAAGGCGTTTCCTGTTTTAATGGTGGCCACCCCAGCTACGCTAAATTACCATGGAAGATCATGTGAAGGACAAGATTGCTAAGATGATCATCATCATTATTACACTATCAACTCTAACCTGAAATCTTTCTTGGCTACTTGGATGAAGGACAGCCTGTATAGTGTAAACTGGTTTTATCAGGTGATATGTCAATTTATCTTAAACACGACGGTTTGCAAATTGGACTACGAGTACTGGCTCTTATTGTTCTTCAATAGATTCGAGGATTTGATGGACGAGTGCTGAAATCTCCCGGACAAACACAGTTGTGATTGTGTTTACAACTGTTTGACGAAAAGGCAAAAGCCCTTGTGTGATTATTTTTCTCCATCCAATTTGGTCTCCTCCAACATCCTGCAGTTTTTGGTGCGGCGGGGTGGTATGGAGAAGCAAATCTTTGCAAAATCTTCAGATATTGCTCAAGGATGTGAAGCAAACGGTTAATAAGAGCACATTGTAGAGGTGCTAAGAAGTGAAATTCTTTGAAGCCATGCCTACCAAAGTGTCACACTCGAATATAAAATTTAAAGTAAAGAAAGTCCCCATATCTCCCTGCTTCCTCAAGTTTTCTAAGTTTTGAATTGTATGAGCCAGGAAGAAACCATTAGTTAAGGTTTTTTTTTTGGGCTGTTCTTGTACAACATGTTTGGAAGGAATGACTTGGTGGGTCGAATGATCTATCTTTTGAATAGTAATAGATGTCTTTATTTACAGGTGTGCACGAATTTAGAAAGATATATTTTTGGTTTATTTTTTATTAGTTGTGAAGTTTTTTACACTCTATTTGAATTATGCAAAGTAGCCTCTTCTTTAAAAGCTCATCTCCATGTAATACTATACATGTGAACCACTGTATTTAACCAATGTACATGGACTGAAATAGGAATATTATTTTATTGCTATTGCATTTAACTGAAGTTGTAATTTCATCAAAATTTAGCTTAACATAAGTGGTTAAATTGATGACTTCTATTAATTGAGATGCATTATTATTAAACGAGTAGAGAGGCATTATTTTAAGCTAATTGAATGTGTATAGCAAAAGGTTTAAATGGAATGAATTCAAATCATGGTAATCATGGTAGGAGTTGGCAAAAAAAAAATATATTGTCCTTTTTTTTATTAAGAATTTTTTTTGCCTATTTGATTTTGCTATTGGGTTTTTCTTTATTTTTATATTTGATATTTTAGGCGATTTATGGGGTTAATTTTTGAATGATTTTTTTATTAAGTTCTAGGAACATTCAATTTCATTTATTATTTAAATTCAAATTTTAAAGATAAAAATCAAGTATCTCTAACAATTATATATTGCCGGAACAAGCGAGTAAATTACAACAAAAGAGGATTAAAAAAAAACAAGTTCGAAGAGGCGTTGCCGACGTGGCAAATTAGTAGCATTAGAGTGAGGATCGGGTTAGCAAAGAACCCTAACAGTATAAAAAGGGGCCAAGAAACCTAGGGTTTCATTCACAGACGTTGCGGCGGCGTCATCCAGAGCAGCTTCATCGGAACTCCGCTACCCTAATCTCGCAGTCGGAGATCATCTTCTCACCCTCACAATGGGGTAAGTTTTGTTTCGTCATTCTAGGTGGTTGCTCTTAATTGTTTATCTAGATGAACGTTCTGGGGAAGGAAGTGTTCTAAGTCGGGATTTTCATTGCTTTACTGGATCTTATCTATAGGAAGACTGATAAGTAAAATTTACTCCACGAACTGAAATGGTTATATTAGTTTCTCGAAGTTGATAGATATATTTAACTCCATGGCAATGGCGACGAAGGATGAATTGCGCTACCCAGTTGCCGTGCTCTCAATCCGAAGTAAATCTGTTGATATTGTCATTGGCTATTGCAAGTGACGCGACCTATTTCGTTTTTCACTGTATAATTGAGGCGTTGTTTGTTACAATCGTTCCTCGGTGTGGGAATAATATTCATTATAGCTGTCTAAGGTTTTTGGATGACAATGCCAGATTACTTGCTTTGTTTGGTTTGCAAAATGTTGAAAGTTTGGACATGCTAACGTATTTCTTTGTGCCCGGATAACAAGTTGGTGACTATGGTTTCAGTTTGATTTAACGAATTTGTGCAACAATAATTTGATTGCACTTGTTTTTTGACACAACAACATTTACAGTATTTGTCTGCCATCACACTGGAAAAATTGGCACAGGCGTTCTTAATTGTGTATATGTATTCTCTTAACTTACAGATAAGAAGAAATACGATTGATGTGAATCTATGTTTTGTTTAGTATTTGTCTGCCACCACACTGGAAAAATTGGCACAAGCATTATTAATGGTGTATATGTATTCTCCTAACCTACAGATAAGAAGAAATACGATTGATGTGAATCTATGTTTTGTTTAGAAGTCACATGTTTAAGTCTGCTATTTGTTTTATGTTAATTGCCTTCATCTAGAGGAAAAATTGACTAGACTTTTGGGGTATTGGGAAAAAGCTCCGTGATTCTATGTTTTTGCTGCACATCGGCAGTTGTATCCTCTTTGTTAAAGCAGTAGTAATATCTTACAGGAAGACACGTGGAATGGGAGCTGGTCGCAAGTTGAAGTCCCACCGTAGAAGGCAAAGGTGGGCTGACAAAGCATACAAGAAATCCCATCTTGGAAACGAGTGGAAGAAGCCATTTGCTGGATCGTCACATGCAAAAGGCATCGTCCTAGAAAAGATGTAAGATGATTTTAGTAGTGCATTTTTTAGGCTTATTAGCTAAAACTAATTTTAAGTTTTAACAAGTAATTCTCTGGTGTAACGTGCAGTGGTATTGAGGCTAAGCAGCCTAACTCTGCTATTAGGAAGTGTGCTAGAGTTCAACTTATTAAGAACGGGAAGAAGATCGCGGCCTTTGTCCCAAATGATGGTTGTTTGAACTACATTGAAGAGAATGTAAGTATTTGCGGGAGTGGGTTGCGACCACTGAGATTTTGGCTACTATTACCTGCTCTCTGTTATTTGTTCAACTTTTGCAATAATATTCATGGTCGTGTACAGGACGAGGTTCTCATTGCTGGATTTGGCCGAAAAGGACATGCTGTTGGTGATATTCCCGGAGTCAGATTTAAGGTGGTGAAGGTGTCAGGTGTATCTCTTCTGGCTCTCTTTAAGGAAAAGAAGGAGAAGCCAAGGTCTTAAGCTTCTCGGTTTTTCAAGATAGATTTTGTCTTCATTTCTTCGTTTCTTGCAATATGCAATTAATTAGATTACTCGGTTGTCTGATATATACTCTTGCGCTGCAATCCTGTTGTTGAGATGTCGCGATTCAAACATAATTTTATTTGCAATCAGAACAGATTCTGTTATGATTAATTTGAGTGGGACACCGCCCCTTCTCTTCCCCCATCAAAATGCCAGGAAGTTTGGTCTTGAAATGATTCTGCTTTATTGCAGTGAATGTGAATGTCCAGCTTTTTTTTCACTTCCATGTTCAATTCTGGGAGAATGATGGATCTTAAAAACTATGCTGTTGATCCTTTCTTTAGTTCCATGAGTTGGATTACTTGGGGGCTGCATTGTGGATGCCCAAGTAATTTTTACAGGCTTGGATTCCACTTGTTTTCTTGATCCACGAGGTACACAAACATTATTCTCTCTCCAAATATGATTCATTTTAATCATATTGGTGTAAGAGTTTGATTTCCTCATTCAGCATGGTTTAAATTCTGAAACCATCCCTTTCAAGATCTCCATTCTTAGTGCATTATTAATCAAGTGCTTCTACAGGAAACACTATACAGTTGGAACATGAAAGCACCTCAGAAATTGGAAATTATGTTTCTTGAAAGCTGTGATATGGGTAGCTGTATTATTTATGCAGATTGGCCCCAAAAAGTAAGGATGCCCAATTAGGTTCTGTGAGATGGGACGTTCTTTTGTTAGGAACAAGGTGA

mRNA sequence

ATGGGGCCAAGATGGAAAGGGAAGGGTTCAGAAGCCAAAGCTCTAGCAGATCCCATGTCGAAGATTGTACTTCAGCTTCAATCTTCTCTAACACAATCGGACGCTCAGGGACTATTGTCCGGATGTAGTGTACTTTATCAAGCAGATGAAGAACAAACCGATCTTTTCAATCGCGCTTGTTTCGGTCGACCCATTGTTACAGCCGATAAGGATAAGCAATGGTTTCAATTGGGCGTGGAGGAGGCGTTTTACTTGTCCTGTCATTTGAAATGCCTCAAAATTGTCGATGGACAAGATTGTGAAAAGAATGCTGAACAGCTGTGGGAGTATATGAAGTCCCAAAAGGCTACGTTCCCTGAGTTTTACAAGGCTTATCAACATCTCCGGATGAAGAATTGGGTTGTCAGACCTGGATCTCAATACGGGGTTGATTTTGTGGCTTATCGTCACCATCCGGCTCTCGTCCACTCTGAATTTGCTGTTCTTGTGTTGTCCGAAGGAGGAGGAGACAGTAACGAAAATGGGCGATTGAGAGTTTGGTCTGATTTTCTCTGCACAATTCGGCTCTGTGGAAGCGTTGCAAAAACGCTGCTAGTTCTTAGCGTCAATAACAATGGAAGCAGTTCTGTTTCTCCTTCATTTTTGGACAGCTACTCCATTGAAGAACGTACGGTTACAAGATGGAGTCCAGAACAGTGCCGTGAAAATCCTGCAAAATTTTTGGTGCGGCGGGGTGGTATGGAGAAGCAAATCTTTGCAAAATCTTCAGATATTGCTCAAGGATGTGAAGCAAACGACGTTGCGGCGGCGTCATCCAGAGCAGCTTCATCGGAACTCCGCTACCCTAATCTCGCAGTCGGAGATCATCTTCTCACCCTCACAATGGGGAAGACACGTGGAATGGGAGCTGGTCGCAAGTTGAAGTCCCACCGTAGAAGGCAAAGGTGGGCTGACAAAGCATACAAGAAATCCCATCTTGGAAACGAGTGGAAGAAGCCATTTGCTGGATCGTCACATGCAAAAGGCATCGTCCTAGAAAAGATTGGTATTGAGGCTAAGCAGCCTAACTCTGCTATTAGGAAGTGTGCTAGAGTTCAACTTATTAAGAACGGGAAGAAGATCGCGGCCTTTGTCCCAAATGATGGTTGTTTGAACTACATTGAAGAGAATGACGAGGTTCTCATTGCTGGATTTGGCCGAAAAGGACATGCTGTTGGTGATATTCCCGGAGTCAGATTTAAGGTGGTGAAGGTGTCAGGTGTATCTCTTCTGGCTCTCTTTAAGGAAAAGAAGGAGAAGCCAAGATTGGCCCCAAAAAGTAAGGATGCCCAATTAGGTTCTGTGAGATGGGACGTTCTTTTGTTAGGAACAAGGTGA

Coding sequence (CDS)

ATGGGGCCAAGATGGAAAGGGAAGGGTTCAGAAGCCAAAGCTCTAGCAGATCCCATGTCGAAGATTGTACTTCAGCTTCAATCTTCTCTAACACAATCGGACGCTCAGGGACTATTGTCCGGATGTAGTGTACTTTATCAAGCAGATGAAGAACAAACCGATCTTTTCAATCGCGCTTGTTTCGGTCGACCCATTGTTACAGCCGATAAGGATAAGCAATGGTTTCAATTGGGCGTGGAGGAGGCGTTTTACTTGTCCTGTCATTTGAAATGCCTCAAAATTGTCGATGGACAAGATTGTGAAAAGAATGCTGAACAGCTGTGGGAGTATATGAAGTCCCAAAAGGCTACGTTCCCTGAGTTTTACAAGGCTTATCAACATCTCCGGATGAAGAATTGGGTTGTCAGACCTGGATCTCAATACGGGGTTGATTTTGTGGCTTATCGTCACCATCCGGCTCTCGTCCACTCTGAATTTGCTGTTCTTGTGTTGTCCGAAGGAGGAGGAGACAGTAACGAAAATGGGCGATTGAGAGTTTGGTCTGATTTTCTCTGCACAATTCGGCTCTGTGGAAGCGTTGCAAAAACGCTGCTAGTTCTTAGCGTCAATAACAATGGAAGCAGTTCTGTTTCTCCTTCATTTTTGGACAGCTACTCCATTGAAGAACGTACGGTTACAAGATGGAGTCCAGAACAGTGCCGTGAAAATCCTGCAAAATTTTTGGTGCGGCGGGGTGGTATGGAGAAGCAAATCTTTGCAAAATCTTCAGATATTGCTCAAGGATGTGAAGCAAACGACGTTGCGGCGGCGTCATCCAGAGCAGCTTCATCGGAACTCCGCTACCCTAATCTCGCAGTCGGAGATCATCTTCTCACCCTCACAATGGGGAAGACACGTGGAATGGGAGCTGGTCGCAAGTTGAAGTCCCACCGTAGAAGGCAAAGGTGGGCTGACAAAGCATACAAGAAATCCCATCTTGGAAACGAGTGGAAGAAGCCATTTGCTGGATCGTCACATGCAAAAGGCATCGTCCTAGAAAAGATTGGTATTGAGGCTAAGCAGCCTAACTCTGCTATTAGGAAGTGTGCTAGAGTTCAACTTATTAAGAACGGGAAGAAGATCGCGGCCTTTGTCCCAAATGATGGTTGTTTGAACTACATTGAAGAGAATGACGAGGTTCTCATTGCTGGATTTGGCCGAAAAGGACATGCTGTTGGTGATATTCCCGGAGTCAGATTTAAGGTGGTGAAGGTGTCAGGTGTATCTCTTCTGGCTCTCTTTAAGGAAAAGAAGGAGAAGCCAAGATTGGCCCCAAAAAGTAAGGATGCCCAATTAGGTTCTGTGAGATGGGACGTTCTTTTGTTAGGAACAAGGTGA

Protein sequence

MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRACFGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPEFYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVWSDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAKFLVRRGGMEKQIFAKSSDIAQGCEANDVAAASSRAASSELRYPNLAVGDHLLTLTMGKTRGMGAGRKLKSHRRRQRWADKAYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQPNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRLAPKSKDAQLGSVRWDVLLLGTR
Homology
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BLAST of Moc02g09330 vs. NCBI nr
Match: XP_022146301.1 (tRNA-splicing endonuclease subunit Sen2-1 [Momordica charantia])

HSP 1 Score: 496.5 bits (1277), Expect = 2.4e-136
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE
Sbjct: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW
Sbjct: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK 240
           SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK 239

[Close]
BLAST of Moc02g09330 vs. NCBI nr
Match: XP_023532286.1 (tRNA-splicing endonuclease subunit Sen2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 446.0 bits (1146), Expect = 3.8e-121
Identity = 216/238 (90.76%), Postives = 226/238 (94.96%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLS CSVLYQADEEQ +L NRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQ G+EEAFYLS HL CLKIVDGQDCEKN ++LW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEKNFDELWHYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G SNENGRLRVW
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEG-SNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPA 239
           SDFLCTIRLCGSVAKTLLVL+VN+NG+ SVSPS LDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

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BLAST of Moc02g09330 vs. NCBI nr
Match: XP_022947593.1 (tRNA-splicing endonuclease subunit Sen2-1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-119
Identity = 214/238 (89.92%), Postives = 226/238 (94.96%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLS CSVLYQADEEQ +L NRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQ G+EEAFYLS +L CLKIVDGQDCEKN ++LW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G SNENGRLRVW
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEG-SNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPA 239
           SDFLCTIRLCGSVAKTLLVL+VN+NG+ SVSPS LDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Moc02g09330 vs. NCBI nr
Match: XP_022947592.1 (tRNA-splicing endonuclease subunit Sen2-1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-119
Identity = 214/238 (89.92%), Postives = 226/238 (94.96%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLS CSVLYQADEEQ +L NRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQ G+EEAFYLS +L CLKIVDGQDCEKN ++LW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G SNENGRLRVW
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEG-SNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPA 239
           SDFLCTIRLCGSVAKTLLVL+VN+NG+ SVSPS LDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Moc02g09330 vs. NCBI nr
Match: KAG6605517.1 (tRNA-splicing endonuclease subunit Sen2-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 438.7 bits (1127), Expect = 6.1e-119
Identity = 214/238 (89.92%), Postives = 224/238 (94.12%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MG RWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLS CSVLYQADEEQ +L NRAC
Sbjct: 1   MGLRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQ G+EEAFYLS HL CLKIVDGQDCEKN ++LW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G  NENGRLRVW
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEG-INENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPA 239
           SDFLCTIRLCGSVAKTLLVL+VN+NG+ SVSPS LDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Moc02g09330 vs. ExPASy Swiss-Prot
Match: P46297 (40S ribosomal protein S23 OS=Fragaria ananassa OX=3747 GN=RPS23 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 8.1e-74
Identity = 138/141 (97.87%), Postives = 140/141 (99.29%), Query Frame = 0 

Query: 295 MGKTRGMGAGRKLKSHRRRQRWADKAYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 354
           MGKTRGMGA RKLK+HRRRQRWADK+YKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ
Sbjct: 1   MGKTRGMGAARKLKNHRRRQRWADKSYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 60

Query: 355 PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 414
           PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK
Sbjct: 61  PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 120

Query: 415 VVKVSGVSLLALFKEKKEKPR 436
           VVKVSGVSLLALFKEKKEKPR
Sbjct: 121 VVKVSGVSLLALFKEKKEKPR 141

[Close]
BLAST of Moc02g09330 vs. ExPASy Swiss-Prot
Match: Q9M5Z9 (40S ribosomal protein S23 OS=Euphorbia esula OX=3993 GN=RPS23 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 6.8e-73
Identity = 137/141 (97.16%), Postives = 138/141 (97.87%), Query Frame = 0 

Query: 295 MGKTRGMGAGRKLKSHRRRQRWADKAYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 354
           MGKT GMGA RKLKSHRR QRWADK+YKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ
Sbjct: 1   MGKTHGMGAARKLKSHRRTQRWADKSYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 60

Query: 355 PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 414
           PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK
Sbjct: 61  PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 120

Query: 415 VVKVSGVSLLALFKEKKEKPR 436
           VVKVSGVSLLALFKEKKEKPR
Sbjct: 121 VVKVSGVSLLALFKEKKEKPR 141

[Close]
BLAST of Moc02g09330 vs. ExPASy Swiss-Prot
Match: P49201 (40S ribosomal protein S23-2 OS=Arabidopsis thaliana OX=3702 GN=RPS23B PE=2 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 2.9e-71
Identity = 136/141 (96.45%), Postives = 136/141 (96.45%), Query Frame = 0 

Query: 295 MGKTRGMGAGRKLKSHRRRQRWADKAYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 354
           MGKTRGMGAGRKLK  R  QRWADK YKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ
Sbjct: 1   MGKTRGMGAGRKLKRLRINQRWADKQYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 60

Query: 355 PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 414
           PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK
Sbjct: 61  PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 120

Query: 415 VVKVSGVSLLALFKEKKEKPR 436
           VVKVSGVSLLALFKEKKEKPR
Sbjct: 121 VVKVSGVSLLALFKEKKEKPR 141

[Close]
BLAST of Moc02g09330 vs. ExPASy Swiss-Prot
Match: Q9M1E8 (tRNA-splicing endonuclease subunit Sen2-1 OS=Arabidopsis thaliana OX=3702 GN=SEN1 PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.1e-69
Identity = 136/242 (56.20%), Postives = 169/242 (69.83%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWK KG+EAKALA+P+SK V +L+SSLTQ++A G LS C+VL   + E+ +L +R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKI-VDGQDCEKNAEQLWEYMKSQKATFP 120
           FGR +V A+KDK+W QL  EEAF+L   LKC+KI + G+  E N   LW  M S K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 121 EFYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRV 180
             YKAY HLR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S GG D     RL+V
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGGND-----RLKV 180

Query: 181 WSDFLCTIRLCGSVAKTLLVLSVNN--NGSSSVSPSFLDSYSIEERTVTRWSPEQCRENP 240
           WSD  C++RL GSVAK+LLVL VN   N      P  L+ Y++EE+T+ RWSPE  RE+ 
Sbjct: 181 WSDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDE 236

[Close]
BLAST of Moc02g09330 vs. ExPASy Swiss-Prot
Match: Q9LSS3 (tRNA-splicing endonuclease subunit Sen2-2 OS=Arabidopsis thaliana OX=3702 GN=SEN2 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 7.8e-69
Identity = 132/238 (55.46%), Postives = 171/238 (71.85%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWK KG+EAKALA+P+SK V +LQ SL ++++ G LS C+VL   + EQ +L +R C
Sbjct: 1   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGR +++A+K K+W QL  EEAF+L  +LKC+KI     C +N    W YMKS++  FP 
Sbjct: 61  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           F+KAY HLR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV S   GDS+   RLRVW
Sbjct: 121 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQS---GDSD---RLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNN--GSSSVSPSFLDSYSIEERTVTRWSPEQCREN 237
           SD  C +RL GSVAKTLL L VN N  G        L+++++EE+T++RWSPE  RE+
Sbjct: 181 SDIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 232

[Close]
BLAST of Moc02g09330 vs. ExPASy TrEMBL
Match: A0A6J1CYW3 (tRNA-intron lyase OS=Momordica charantia OX=3673 GN=LOC111015540 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 1.2e-136
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE
Sbjct: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW
Sbjct: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK 240
           SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK 239

[Close]
BLAST of Moc02g09330 vs. ExPASy TrEMBL
Match: A0A6J1G716 (tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 5.9e-120
Identity = 214/238 (89.92%), Postives = 226/238 (94.96%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLS CSVLYQADEEQ +L NRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQ G+EEAFYLS +L CLKIVDGQDCEKN ++LW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G SNENGRLRVW
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEG-SNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPA 239
           SDFLCTIRLCGSVAKTLLVL+VN+NG+ SVSPS LDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Moc02g09330 vs. ExPASy TrEMBL
Match: A0A6J1G6W2 (tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 5.9e-120
Identity = 214/238 (89.92%), Postives = 226/238 (94.96%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLS CSVLYQADEEQ +L NRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQ G+EEAFYLS +L CLKIVDGQDCEKN ++LW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G SNENGRLRVW
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEG-SNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPA 239
           SDFLCTIRLCGSVAKTLLVL+VN+NG+ SVSPS LDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Moc02g09330 vs. ExPASy TrEMBL
Match: A0A6J1FWV5 (tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111448962 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 3.8e-119
Identity = 212/239 (88.70%), Postives = 224/239 (93.72%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           MGPRWKGK SEA+ALADPMSKIVLQLQSSLTQSDAQGLLSGCS+LYQADEEQTDL NRAC
Sbjct: 44  MGPRWKGKASEARALADPMSKIVLQLQSSLTQSDAQGLLSGCSILYQADEEQTDLLNRAC 103

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGR I+TA+KDKQWFQ G+EEAFYLS HL CLKIVDGQD EKN ++LW+ MKS+KATFPE
Sbjct: 104 FGRTIITAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDREKNVQELWQDMKSRKATFPE 163

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FY AYQHLRMKNW+VRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG SNEN RLRVW
Sbjct: 164 FYMAYQHLRMKNWIVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG-SNENVRLRVW 223

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK 240
           SDFLCTIRLCGSVAKTLLVLSVN+NGS SVSPS LD+YSIEERTVTRWSPEQCRENP K
Sbjct: 224 SDFLCTIRLCGSVAKTLLVLSVNDNGSGSVSPSCLDNYSIEERTVTRWSPEQCRENPEK 281

[Close]
BLAST of Moc02g09330 vs. ExPASy TrEMBL
Match: A0A6J1JBL2 (tRNA-intron lyase OS=Cucurbita maxima OX=3661 GN=LOC111483578 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 5.0e-119
Identity = 212/239 (88.70%), Postives = 223/239 (93.31%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWKGK SEA+ALADPMSKIVLQLQSSLTQSDAQGLLSGCS+LYQADEEQTDL NRAC
Sbjct: 44  MEPRWKGKASEARALADPMSKIVLQLQSSLTQSDAQGLLSGCSILYQADEEQTDLLNRAC 103

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGR I+TAD DKQWFQ G+EEAFYLS HL CLKIVDGQD EKN ++LW+ MKSQKATFPE
Sbjct: 104 FGRTIITADMDKQWFQFGMEEAFYLSYHLNCLKIVDGQDREKNVQELWQDMKSQKATFPE 163

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           FY AYQHLRMKNW+VRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG SNENGRLRVW
Sbjct: 164 FYMAYQHLRMKNWIVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG-SNENGRLRVW 223

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAK 240
           SDFLCTIRLCGSVAKTLLVLSVN+NG+ SVSPS LDS+SIEERTVTRWSPEQCRENP K
Sbjct: 224 SDFLCTIRLCGSVAKTLLVLSVNDNGNGSVSPSCLDSFSIEERTVTRWSPEQCRENPEK 281

[Close]
BLAST of Moc02g09330 vs. TAIR 10
Match: AT5G02960.1 (Ribosomal protein S12/S23 family protein )

HSP 1 Score: 270.8 bits (691), Expect = 2.0e-72
Identity = 136/141 (96.45%), Postives = 136/141 (96.45%), Query Frame = 0 

Query: 295 MGKTRGMGAGRKLKSHRRRQRWADKAYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 354
           MGKTRGMGAGRKLK  R  QRWADK YKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ
Sbjct: 1   MGKTRGMGAGRKLKRLRINQRWADKQYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQ 60

Query: 355 PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 414
           PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK
Sbjct: 61  PNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFK 120

Query: 415 VVKVSGVSLLALFKEKKEKPR 436
           VVKVSGVSLLALFKEKKEKPR
Sbjct: 121 VVKVSGVSLLALFKEKKEKPR 141

[Close]
BLAST of Moc02g09330 vs. TAIR 10
Match: AT3G45590.1 (splicing endonuclease 1 )

HSP 1 Score: 264.6 bits (675), Expect = 1.5e-70
Identity = 136/242 (56.20%), Postives = 169/242 (69.83%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWK KG+EAKALA+P+SK V +L+SSLTQ++A G LS C+VL   + E+ +L +R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKI-VDGQDCEKNAEQLWEYMKSQKATFP 120
           FGR +V A+KDK+W QL  EEAF+L   LKC+KI + G+  E N   LW  M S K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 121 EFYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRV 180
             YKAY HLR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S GG D     RL+V
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGGND-----RLKV 180

Query: 181 WSDFLCTIRLCGSVAKTLLVLSVNN--NGSSSVSPSFLDSYSIEERTVTRWSPEQCRENP 240
           WSD  C++RL GSVAK+LLVL VN   N      P  L+ Y++EE+T+ RWSPE  RE+ 
Sbjct: 181 WSDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDE 236

[Close]
BLAST of Moc02g09330 vs. TAIR 10
Match: AT3G45590.2 (splicing endonuclease 1 )

HSP 1 Score: 264.6 bits (675), Expect = 1.5e-70
Identity = 136/242 (56.20%), Postives = 169/242 (69.83%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWK KG+EAKALA+P+SK V +L+SSLTQ++A G LS C+VL   + E+ +L +R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKI-VDGQDCEKNAEQLWEYMKSQKATFP 120
           FGR +V A+KDK+W QL  EEAF+L   LKC+KI + G+  E N   LW  M S K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 121 EFYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRV 180
             YKAY HLR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S GG D     RL+V
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGGND-----RLKV 180

Query: 181 WSDFLCTIRLCGSVAKTLLVLSVNN--NGSSSVSPSFLDSYSIEERTVTRWSPEQCRENP 240
           WSD  C++RL GSVAK+LLVL VN   N      P  L+ Y++EE+T+ RWSPE  RE+ 
Sbjct: 181 WSDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDE 236

[Close]
BLAST of Moc02g09330 vs. TAIR 10
Match: AT5G60230.1 (splicing endonuclease 2 )

HSP 1 Score: 262.7 bits (670), Expect = 5.5e-70
Identity = 132/238 (55.46%), Postives = 171/238 (71.85%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWK KG+EAKALA+P+SK V +LQ SL ++++ G LS C+VL   + EQ +L +R C
Sbjct: 1   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 60

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGR +++A+K K+W QL  EEAF+L  +LKC+KI     C +N    W YMKS++  FP 
Sbjct: 61  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 120

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           F+KAY HLR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV S   GDS+   RLRVW
Sbjct: 121 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQS---GDSD---RLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNN--GSSSVSPSFLDSYSIEERTVTRWSPEQCREN 237
           SD  C +RL GSVAKTLL L VN N  G        L+++++EE+T++RWSPE  RE+
Sbjct: 181 SDIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 232

[Close]
BLAST of Moc02g09330 vs. TAIR 10
Match: AT5G60230.2 (splicing endonuclease 2 )

HSP 1 Score: 262.7 bits (670), Expect = 5.5e-70
Identity = 132/238 (55.46%), Postives = 171/238 (71.85%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60
           M PRWK KG+EAKALA+P+SK V +LQ SL ++++ G LS C+VL   + EQ +L +R C
Sbjct: 6   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 65

Query: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120
           FGR +++A+K K+W QL  EEAF+L  +LKC+KI     C +N    W YMKS++  FP 
Sbjct: 66  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 125

Query: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180
           F+KAY HLR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV S   GDS+   RLRVW
Sbjct: 126 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQS---GDSD---RLRVW 185

Query: 181 SDFLCTIRLCGSVAKTLLVLSVNNN--GSSSVSPSFLDSYSIEERTVTRWSPEQCREN 237
           SD  C +RL GSVAKTLL L VN N  G        L+++++EE+T++RWSPE  RE+
Sbjct: 186 SDIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 237

The following BLAST results are available for this feature:
BLAST of Moc02g09330 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022146301.12.4e-136100.00tRNA-splicing endonuclease subunit Sen2-1 [Momordica charantia][more]
XP_023532286.13.8e-12190.76tRNA-splicing endonuclease subunit Sen2-1-like [Cucurbita pepo subsp. pepo][more]
XP_022947593.11.2e-11989.92tRNA-splicing endonuclease subunit Sen2-1-like isoform X2 [Cucurbita moschata][more]
XP_022947592.11.2e-11989.92tRNA-splicing endonuclease subunit Sen2-1-like isoform X1 [Cucurbita moschata][more]
KAG6605517.16.1e-11989.92tRNA-splicing endonuclease subunit Sen2-2, partial [Cucurbita argyrosperma subsp... [more]
BLAST of Moc02g09330 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P462978.1e-7497.8740S ribosomal protein S23 OS=Fragaria ananassa OX=3747 GN=RPS23 PE=2 SV=1[more]
Q9M5Z96.8e-7397.1640S ribosomal protein S23 OS=Euphorbia esula OX=3993 GN=RPS23 PE=2 SV=1[more]
P492012.9e-7196.4540S ribosomal protein S23-2 OS=Arabidopsis thaliana OX=3702 GN=RPS23B PE=2 SV=2[more]
Q9M1E82.1e-6956.20tRNA-splicing endonuclease subunit Sen2-1 OS=Arabidopsis thaliana OX=3702 GN=SEN... [more]
Q9LSS37.8e-6955.46tRNA-splicing endonuclease subunit Sen2-2 OS=Arabidopsis thaliana OX=3702 GN=SEN... [more]
BLAST of Moc02g09330 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CYW31.2e-136100.00tRNA-intron lyase OS=Momordica charantia OX=3673 GN=LOC111015540 PE=3 SV=1[more]
A0A6J1G7165.9e-12089.92tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1[more]
A0A6J1G6W25.9e-12089.92tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1[more]
A0A6J1FWV53.8e-11988.70tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111448962 PE=3 SV=1[more]
A0A6J1JBL25.0e-11988.70tRNA-intron lyase OS=Cucurbita maxima OX=3661 GN=LOC111483578 PE=3 SV=1[more]
BLAST of Moc02g09330 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G02960.12.0e-7296.45Ribosomal protein S12/S23 family protein [more]
AT3G45590.11.5e-7056.20splicing endonuclease 1 [more]
AT3G45590.21.5e-7056.20splicing endonuclease 1 [more]
AT5G60230.15.5e-7055.46splicing endonuclease 2 [more]
AT5G60230.25.5e-7055.46splicing endonuclease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006678tRNA intron endonuclease, N-terminalPFAMPF02778tRNA_int_endo_Ncoord: 36..108
e-value: 3.8E-12
score: 45.7
IPR006677tRNA intron endonuclease, catalytic domain-likePFAMPF01974tRNA_int_endocoord: 121..207
e-value: 9.8E-19
score: 67.2
NoneNo IPR availableGENE3D2.40.50.140coord: 319..436
e-value: 1.6E-45
score: 155.4
NoneNo IPR availablePANTHERPTHR21227:SF2TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2-1-LIKEcoord: 1..240
IPR006032Ribosomal protein S12/S23PFAMPF00164Ribosom_S12_S23coord: 325..435
e-value: 1.1E-48
score: 163.5
IPR006032Ribosomal protein S12/S23PROSITEPS00055RIBOSOMAL_S12coord: 353..360
IPR005680Ribosomal protein S23, eukaryotic/archaealTIGRFAMTIGR00982TIGR00982coord: 300..435
e-value: 1.7E-51
score: 171.5
IPR005680Ribosomal protein S23, eukaryotic/archaealCDDcd03367Ribosomal_S23coord: 320..433
e-value: 8.12171E-79
score: 238.323
IPR011856tRNA endonuclease-like domain superfamilyGENE3D3.40.1350.10coord: 117..214
e-value: 6.5E-23
score: 82.6
IPR006676tRNA-splicing endonucleasePANTHERPTHR21227TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2coord: 1..240
IPR036167tRNA intron endonuclease, catalytic domain-like superfamilySUPERFAMILY53032tRNA-intron endonuclease catalytic domain-likecoord: 118..207
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 314..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g09330.1Moc02g09330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0006412 translation
biological_process GO:0000379 tRNA-type intron splice site recognition and cleavage
biological_process GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
cellular_component GO:0015935 small ribosomal subunit
cellular_component GO:0000214 tRNA-intron endonuclease complex
cellular_component GO:0005840 ribosome
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003735 structural constituent of ribosome
molecular_function GO:0000213 tRNA-intron endonuclease activity
molecular_function GO:0004518 nuclease activity