Moc02g09160 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g09160
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionchitinase 2-like
Locationchr2: 6480465 .. 6481379 (+)
RNA-Seq ExpressionMoc02g09160
SyntenyMoc02g09160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTCGAATATTCTTCCTATTCTCACTTCTACAATTCCTAAGCGCCATACTTCCATCACTAGAGGCAGCCCCCAAAAATAACACACGACTCTTCAGAGAATACATAGGAGCTGATGGAAATGTAAGATTTTCAGACGTACCTGTTCATCCTGATGTAGAGTTCCACTTCATTCTCTCCTTTGCCATCGATTACACCGGTTTATCTTCTCCTTCTGCGACCAACGGCAAATTCAGAGTCTTCTGGGACGAACAAAACCTCTCGCCTTCTTCTGTTTCTTCCATCAAGGCTCGGCATTCAAACATCAAGTTTGCTGTCAGCCTCGGGGGTGATACGGTCGGCAAAGAATTTGCTTACTTCAAACCCAAATCTGTCAAATCTTGGGTTAAAAATGCTTTCAAGTCAATCTCTCGTATAGTGAAGGAGTATCATCTGGATGGGGTTGACATCGATTATGAACACTTCAAAGCAGACCCCGATACATTTACCGAGTGCATAGGGCAGCTCCTACTTCGTCTGAAACACCATAATGTCATCTCGTTTGCATCCATAGCACCGTTCGAGGATGACACTGTGCAGTCTCATTACTTAGCTTTGTGGAGGAAGTACGGACATCTAATAGACTATGTCAACTTCCAGTTCTATTCCTATGATAAAGGTACCACAGTTTCTCAGTTTGTGAATCATTTTAAGACCCAAAGCTCCAATTATCAAGGTGGAAATGTTCTAGCAAGCTTTGCTACAGATGGAAGTGGCGGTTTGACACCGGAAAAAGGGTTCTTTAAAGCATGCAGCATCCTTCAAAGCAGGGGCGAACTTCATGGTATATTTGTTTGGTCAGCAGATGACTCAAAGAAGAACAATTTTCGTCATGAGAAACAAGCACAAAACCTTTTGGCCAACGCAGAATGA

mRNA sequence

ATGGGTTCTCGAATATTCTTCCTATTCTCACTTCTACAATTCCTAAGCGCCATACTTCCATCACTAGAGGCAGCCCCCAAAAATAACACACGACTCTTCAGAGAATACATAGGAGCTGATGGAAATGTAAGATTTTCAGACGTACCTGTTCATCCTGATGTAGAGTTCCACTTCATTCTCTCCTTTGCCATCGATTACACCGGTTTATCTTCTCCTTCTGCGACCAACGGCAAATTCAGAGTCTTCTGGGACGAACAAAACCTCTCGCCTTCTTCTGTTTCTTCCATCAAGGCTCGGCATTCAAACATCAAGTTTGCTGTCAGCCTCGGGGGTGATACGGTCGGCAAAGAATTTGCTTACTTCAAACCCAAATCTGTCAAATCTTGGGTTAAAAATGCTTTCAAGTCAATCTCTCGTATAGTGAAGGAGTATCATCTGGATGGGGTTGACATCGATTATGAACACTTCAAAGCAGACCCCGATACATTTACCGAGTGCATAGGGCAGCTCCTACTTCGTCTGAAACACCATAATGTCATCTCGTTTGCATCCATAGCACCGTTCGAGGATGACACTGTGCAGTCTCATTACTTAGCTTTGTGGAGGAAGTACGGACATCTAATAGACTATGTCAACTTCCAGTTCTATTCCTATGATAAAGGTACCACAGTTTCTCAGTTTGTGAATCATTTTAAGACCCAAAGCTCCAATTATCAAGGTGGAAATGTTCTAGCAAGCTTTGCTACAGATGGAAGTGGCGGTTTGACACCGGAAAAAGGGTTCTTTAAAGCATGCAGCATCCTTCAAAGCAGGGGCGAACTTCATGGTATATTTGTTTGGTCAGCAGATGACTCAAAGAAGAACAATTTTCGTCATGAGAAACAAGCACAAAACCTTTTGGCCAACGCAGAATGA

Coding sequence (CDS)

ATGGGTTCTCGAATATTCTTCCTATTCTCACTTCTACAATTCCTAAGCGCCATACTTCCATCACTAGAGGCAGCCCCCAAAAATAACACACGACTCTTCAGAGAATACATAGGAGCTGATGGAAATGTAAGATTTTCAGACGTACCTGTTCATCCTGATGTAGAGTTCCACTTCATTCTCTCCTTTGCCATCGATTACACCGGTTTATCTTCTCCTTCTGCGACCAACGGCAAATTCAGAGTCTTCTGGGACGAACAAAACCTCTCGCCTTCTTCTGTTTCTTCCATCAAGGCTCGGCATTCAAACATCAAGTTTGCTGTCAGCCTCGGGGGTGATACGGTCGGCAAAGAATTTGCTTACTTCAAACCCAAATCTGTCAAATCTTGGGTTAAAAATGCTTTCAAGTCAATCTCTCGTATAGTGAAGGAGTATCATCTGGATGGGGTTGACATCGATTATGAACACTTCAAAGCAGACCCCGATACATTTACCGAGTGCATAGGGCAGCTCCTACTTCGTCTGAAACACCATAATGTCATCTCGTTTGCATCCATAGCACCGTTCGAGGATGACACTGTGCAGTCTCATTACTTAGCTTTGTGGAGGAAGTACGGACATCTAATAGACTATGTCAACTTCCAGTTCTATTCCTATGATAAAGGTACCACAGTTTCTCAGTTTGTGAATCATTTTAAGACCCAAAGCTCCAATTATCAAGGTGGAAATGTTCTAGCAAGCTTTGCTACAGATGGAAGTGGCGGTTTGACACCGGAAAAAGGGTTCTTTAAAGCATGCAGCATCCTTCAAAGCAGGGGCGAACTTCATGGTATATTTGTTTGGTCAGCAGATGACTCAAAGAAGAACAATTTTCGTCATGAGAAACAAGCACAAAACCTTTTGGCCAACGCAGAATGA

Protein sequence

MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLLANAE
Homology
BLAST of Moc02g09160 vs. NCBI nr
Match: XP_022146270.1 (chitinase 2-like [Momordica charantia])

HSP 1 Score: 620.2 bits (1598), Expect = 9.7e-174
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 60
           MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL
Sbjct: 19  MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 78

Query: 61  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 120
           SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY
Sbjct: 79  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 138

Query: 121 FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 180
           FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI
Sbjct: 139 FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 198

Query: 181 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 240
           SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG
Sbjct: 199 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 258

Query: 241 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 300
           GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL
Sbjct: 259 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 318

Query: 301 ANAE 305
           ANAE
Sbjct: 319 ANAE 322

BLAST of Moc02g09160 vs. NCBI nr
Match: XP_038902883.1 (chitinase 2-like [Benincasa hispida])

HSP 1 Score: 528.1 bits (1359), Expect = 5.0e-146
Identity = 262/307 (85.34%), Postives = 283/307 (92.18%), Query Frame = 0

Query: 1   MGSRIFFLFSLLQFLS--AILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFH 60
           MGSRIFFL+S LQFLS  AILPSL+AAP     LFREYIGA+G N+RFSDVP+H DVEFH
Sbjct: 1   MGSRIFFLYSFLQFLSVFAILPSLKAAP-----LFREYIGAEGINIRFSDVPIHSDVEFH 60

Query: 61  FILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKE 120
           FILSFAIDYTG SSPSATNGKFRVFWDE+NLSPSSVSSIKA+HSN+KFA+SLGGDTVGKE
Sbjct: 61  FILSFAIDYTGSSSPSATNGKFRVFWDEENLSPSSVSSIKAKHSNVKFALSLGGDTVGKE 120

Query: 121 FAYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHH 180
           FA+FKPKS+ SWV NAFKSISRIVK+Y LDGVDIDYEHFKA PD FTECIGQLLLRLK H
Sbjct: 121 FAFFKPKSITSWVNNAFKSISRIVKKYDLDGVDIDYEHFKAHPDIFTECIGQLLLRLKQH 180

Query: 181 NVISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSN 240
           N+ISFASIAPFEDDTVQSHY+ALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQSSN
Sbjct: 181 NLISFASIAPFEDDTVQSHYMALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQSSN 240

Query: 241 YQGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQ 300
           Y+GG VLASFATDGSGGLTPEKGFFKACS LQSRG LHGIFVWSADDSKKN+F +E QAQ
Sbjct: 241 YEGGKVLASFATDGSGGLTPEKGFFKACSTLQSRGNLHGIFVWSADDSKKNDFHNEIQAQ 300

Query: 301 NLLANAE 305
           NLLANA+
Sbjct: 301 NLLANAK 302

BLAST of Moc02g09160 vs. NCBI nr
Match: XP_022944087.1 (chitinase 2-like [Cucurbita moschata])

HSP 1 Score: 521.5 bits (1342), Expect = 4.7e-144
Identity = 257/306 (83.99%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS L  L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HPDVEFHF
Sbjct: 18  MGSAQIFFLFSFLPLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPDVEFHF 77

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATNGKFRVFWDEQ LSPSSVSSIKA+HSN+KFA+SLGGDT GK+F
Sbjct: 78  ILSFAIDYTASSSPSATNGKFRVFWDEQILSPSSVSSIKAQHSNVKFALSLGGDTAGKQF 137

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEY +DGVDIDYEHFKA PD FTECIG+LLLRLK  N
Sbjct: 138 AYFKPKSITSWVKNAFESISRIVKEYDIDGVDIDYEHFKAHPDVFTECIGKLLLRLKRQN 197

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQ+HYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 198 VISFASIAPFDDDAVQTHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 257

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 258 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 317

Query: 301 LLANAE 305
           LLANA+
Sbjct: 318 LLANAD 323

BLAST of Moc02g09160 vs. NCBI nr
Match: KAG6570744.1 (hypothetical protein SDJN03_29659, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.5 bits (1342), Expect = 4.7e-144
Identity = 257/306 (83.99%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +I FLFS LQ L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HPDVEFHF
Sbjct: 1   MGSAQIIFLFSFLQLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPDVEFHF 60

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATNGKFRVFWDEQ LSPSSVSSIKA+HSN+KFA+SLGGDT GK+F
Sbjct: 61  ILSFAIDYTAASSPSATNGKFRVFWDEQILSPSSVSSIKAQHSNVKFALSLGGDTAGKQF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEY LDGVDIDYEHFKA PD FTEC+G+LLLRLK  N
Sbjct: 121 AYFKPKSITSWVKNAFESISRIVKEYDLDGVDIDYEHFKAHPDVFTECMGKLLLRLKRQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQ+HYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 181 VISFASIAPFDDDAVQTHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 241 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 300

Query: 301 LLANAE 305
           LLANA+
Sbjct: 301 LLANAD 306

BLAST of Moc02g09160 vs. NCBI nr
Match: XP_022986910.1 (chitinase 2-like [Cucurbita maxima])

HSP 1 Score: 521.2 bits (1341), Expect = 6.2e-144
Identity = 258/306 (84.31%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS LQ L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HP+VE HF
Sbjct: 1   MGSAQIFFLFSFLQLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPEVELHF 60

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATN KFRVFWD+Q LSPSSVSSIKA+HSNIKFA+SLGGDTVGK+F
Sbjct: 61  ILSFAIDYTASSSPSATNEKFRVFWDDQILSPSSVSSIKAQHSNIKFALSLGGDTVGKQF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEY LDGVDIDYEHFKA PD FTECIG+LLLRLKH N
Sbjct: 121 AYFKPKSITSWVKNAFESISRIVKEYDLDGVDIDYEHFKAHPDVFTECIGKLLLRLKHQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQSHYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 181 VISFASIAPFDDDAVQSHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 241 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 300

Query: 301 LLANAE 305
           LLAN +
Sbjct: 301 LLANTD 306

BLAST of Moc02g09160 vs. ExPASy Swiss-Prot
Match: Q9SLP4 (Chitinase 1 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 1.8e-106
Identity = 184/295 (62.37%), Postives = 232/295 (78.64%), Query Frame = 0

Query: 11  LLQFLSAILPSLEAAPKNNTRLFREYIGADGN-VRFSDVPVHPDVEFHFILSFAIDYTGL 70
           LL F + + P L   P  +  +FREYIG+  N V+FSDVP++PDV+FHFIL+FAIDYT  
Sbjct: 10  LLLFFALLSPLL---PLTSALVFREYIGSQFNDVKFSDVPINPDVDFHFILAFAIDYTSG 69

Query: 71  SSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAYFKPKSVKSW 130
           SSP+ TNG F+ FWD  NLSPS V+++K  HSN+K ++SLGGD+VG +  +F P SV SW
Sbjct: 70  SSPTPTNGNFKPFWDTNNLSPSQVAAVKRTHSNVKVSLSLGGDSVGGKNVFFSPSSVSSW 129

Query: 131 VKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFE 190
           V+NA  S++RI+K+YHLDG+DIDYEHFK DP+TF ECIGQL+ RLK + V+SF SIAPF+
Sbjct: 130 VENAVSSLTRIIKQYHLDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNEVVSFVSIAPFD 189

Query: 191 DDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQGGNVLASFAT 250
           D  VQSHY ALW KYGH IDYVNFQFY+Y   T+V QF+ +F+ QSSNY GG VL SF+T
Sbjct: 190 DAQVQSHYQALWEKYGHQIDYVNFQFYAYSARTSVEQFLKYFEEQSSNYHGGKVLVSFST 249

Query: 251 DGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNN--FRHEKQAQNLLAN 303
           D SGGL P+ GFF+ACSIL+ +G+LHGIFVWSADDS  +N  FR+E QAQ++LA+
Sbjct: 250 DSSGGLKPDNGFFRACSILKKQGKLHGIFVWSADDSLMSNNVFRYEMQAQSMLAS 301

BLAST of Moc02g09160 vs. ExPASy Swiss-Prot
Match: Q7M443 (Chitinase 2 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.3e-101
Identity = 176/274 (64.23%), Postives = 216/274 (78.83%), Query Frame = 0

Query: 32  LFREYIGADGN-VRFSDVPVHPDVEFHFILSFAIDYTGLSSPSATNGKFRVFWDEQNLSP 91
           LFREYIGA  N V+FSDVP++P+V+FHFIL+FAIDYT  SSP+ TNG F  FWD  NLSP
Sbjct: 2   LFREYIGAQFNDVKFSDVPINPNVDFHFILAFAIDYTSGSSPTPTNGNFNPFWDTNNLSP 61

Query: 92  SSVSSIKARHSNIKFAVSLGGDTVGKEFAYFKPKSVKSWVKNAFKSISRIVKEYHLDGVD 151
           S V++IK  ++N+K +VSLGG++VG E  +F P SV SWV NA  S+++I+K+YHLDG+D
Sbjct: 62  SQVAAIKRTYNNVKVSVSLGGNSVGGERVFFNPSSVSSWVDNAVSSLTKIIKQYHLDGID 121

Query: 152 IDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFEDDTVQSHYLALWRKYGHLIDY 211
           IDYEHFK DP+TF ECIGQL+ RLK + V+SF SIAPF+D  VQSHY ALW KYGH IDY
Sbjct: 122 IDYEHFKGDPNTFAECIGQLVTRLKKNGVVSFVSIAPFDDAQVQSHYQALWEKYGHQIDY 181

Query: 212 VNFQFYSYDKGTTVSQFVNHFKTQSSNYQGGNVLASFATDGSGGLTPEKGFFKACSILQS 271
           VNFQFY Y    +V QF+ +F+ Q SNY GG VL SF+TD SGGL P  GFF ACSIL+ 
Sbjct: 182 VNFQFYVYSSRMSVEQFLKYFEMQRSNYPGGKVLVSFSTDNSGGLKPRNGFFDACSILKK 241

Query: 272 RGELHGIFVWSADDSKKNN--FRHEKQAQNLLAN 303
           +G+LHGIFVWSADDS  +N  F++E QAQ+LLA+
Sbjct: 242 QGKLHGIFVWSADDSLMSNDVFKYEMQAQSLLAS 275

BLAST of Moc02g09160 vs. ExPASy Swiss-Prot
Match: P39657 (RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.2e-19
Identity = 58/187 (31.02%), Postives = 89/187 (47.59%), Query Frame = 0

Query: 31  RLFREYIGADGNVRFSDVPVHPDVEFHFILSFAIDYTGLSSPSATNGKFRVFWDEQNLSP 90
           ++FRE+   D  +  + V      EF   LS A DY G +S   TNGKF  +WD + ++P
Sbjct: 6   KVFREFTSDDSFL--NQVIPENITEFQVTLSLARDYDGNNS---TNGKFIPYWDTEKVTP 65

Query: 91  SSVSSIKARHS----NIKFAVSLGGDTVGKEFAYFKPKSVKSWVKNAFKSISRIVKEYHL 150
             +   K ++      +K  VS+G       F      + ++WV  A  S+  I+K Y+L
Sbjct: 66  EVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNL 125

Query: 151 DGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVISFASIAPFEDDTVQSHYLALWRKYGH 210
           DG+D+ YE   A+   F   +G L+  LK + +I+ AS A   D      Y  L+ +Y  
Sbjct: 126 DGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNLLYAEYAT 185

Query: 211 LIDYVNF 214
             D V F
Sbjct: 186 FFDTVVF 187

BLAST of Moc02g09160 vs. ExPASy TrEMBL
Match: A0A6J1CWU0 (chitinase 2-like OS=Momordica charantia OX=3673 GN=LOC111015520 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 4.7e-174
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 60
           MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL
Sbjct: 19  MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADGNVRFSDVPVHPDVEFHFIL 78

Query: 61  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 120
           SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY
Sbjct: 79  SFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEFAY 138

Query: 121 FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 180
           FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI
Sbjct: 139 FKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHNVI 198

Query: 181 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 240
           SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG
Sbjct: 199 SFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNYQG 258

Query: 241 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 300
           GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL
Sbjct: 259 GNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQNLL 318

Query: 301 ANAE 305
           ANAE
Sbjct: 319 ANAE 322

BLAST of Moc02g09160 vs. ExPASy TrEMBL
Match: A0A6J1FUT9 (chitinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448631 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-144
Identity = 257/306 (83.99%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS L  L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HPDVEFHF
Sbjct: 18  MGSAQIFFLFSFLPLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPDVEFHF 77

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATNGKFRVFWDEQ LSPSSVSSIKA+HSN+KFA+SLGGDT GK+F
Sbjct: 78  ILSFAIDYTASSSPSATNGKFRVFWDEQILSPSSVSSIKAQHSNVKFALSLGGDTAGKQF 137

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEY +DGVDIDYEHFKA PD FTECIG+LLLRLK  N
Sbjct: 138 AYFKPKSITSWVKNAFESISRIVKEYDIDGVDIDYEHFKAHPDVFTECIGKLLLRLKRQN 197

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQ+HYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 198 VISFASIAPFDDDAVQTHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 257

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 258 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 317

Query: 301 LLANAE 305
           LLANA+
Sbjct: 318 LLANAD 323

BLAST of Moc02g09160 vs. ExPASy TrEMBL
Match: A0A6J1JFC6 (chitinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484508 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 258/306 (84.31%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MGS-RIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGADG-NVRFSDVPVHPDVEFHF 60
           MGS +IFFLFS LQ L   LPSLEA P +NTRLFREYIGADG NVRFSDVP+HP+VE HF
Sbjct: 1   MGSAQIFFLFSFLQLLFPTLPSLEATPLDNTRLFREYIGADGNNVRFSDVPIHPEVELHF 60

Query: 61  ILSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKEF 120
           ILSFAIDYT  SSPSATN KFRVFWD+Q LSPSSVSSIKA+HSNIKFA+SLGGDTVGK+F
Sbjct: 61  ILSFAIDYTASSSPSATNEKFRVFWDDQILSPSSVSSIKAQHSNIKFALSLGGDTVGKQF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
           AYFKPKS+ SWVKNAF+SISRIVKEY LDGVDIDYEHFKA PD FTECIG+LLLRLKH N
Sbjct: 121 AYFKPKSITSWVKNAFESISRIVKEYDLDGVDIDYEHFKAHPDVFTECIGKLLLRLKHQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           VISFASIAPF+DD VQSHYLALWRKYG LIDYVNFQFY+Y KGTTVSQF+NHF+TQ+SNY
Sbjct: 181 VISFASIAPFDDDAVQSHYLALWRKYGDLIDYVNFQFYAYGKGTTVSQFLNHFETQTSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           QGG VLASFATDGSGGLTPEKGFFKAC  LQSRG+LHGIFVWSADDSKKN F +E QAQN
Sbjct: 241 QGGKVLASFATDGSGGLTPEKGFFKACRTLQSRGKLHGIFVWSADDSKKNKFHNEIQAQN 300

Query: 301 LLANAE 305
           LLAN +
Sbjct: 301 LLANTD 306

BLAST of Moc02g09160 vs. ExPASy TrEMBL
Match: A0A5D3CQZ2 (Chitinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00970 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.1e-144
Identity = 257/306 (83.99%), Postives = 276/306 (90.20%), Query Frame = 0

Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGA-DGNVRFSDVPVHPDVEFHFI 60
           MGSRIFFLFS L FL AILPS++A P  NTRLFREYIGA D NVRFSDVP+H DVEFHFI
Sbjct: 1   MGSRIFFLFSFLGFLFAILPSVKAEPLKNTRLFREYIGAEDKNVRFSDVPIHSDVEFHFI 60

Query: 61  LSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKE-F 120
           LSFAIDYTG SSPS TNGKF+VFWDE+NL+PSS+SSIKA+HSN+KFA+SLGGDTVG   F
Sbjct: 61  LSFAIDYTGSSSPSPTNGKFKVFWDEKNLTPSSISSIKAKHSNVKFALSLGGDTVGDGIF 120

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
            +FKPKS+ SWV NAFKSIS IVKEY LDGVDIDYEHFKA PDTFTECIGQLL RLK  N
Sbjct: 121 VFFKPKSITSWVNNAFKSISDIVKEYDLDGVDIDYEHFKASPDTFTECIGQLLQRLKSQN 180

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           +ISFASIAPF+DDTVQSHY+ALWRKYG LIDYVNFQFYSY KGTTVSQF+NHF TQSSNY
Sbjct: 181 IISFASIAPFDDDTVQSHYMALWRKYGDLIDYVNFQFYSYGKGTTVSQFLNHFDTQSSNY 240

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           +GG VLASFATDGSGGL PEKGFFKAC+ LQSRGELHGIFVWSADDSKKNNFR+E QAQN
Sbjct: 241 EGGKVLASFATDGSGGLKPEKGFFKACNTLQSRGELHGIFVWSADDSKKNNFRNEIQAQN 300

Query: 301 LLANAE 305
           LLANAE
Sbjct: 301 LLANAE 306

BLAST of Moc02g09160 vs. ExPASy TrEMBL
Match: A0A1S3CLR6 (chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103501894 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.1e-144
Identity = 257/306 (83.99%), Postives = 276/306 (90.20%), Query Frame = 0

Query: 1   MGSRIFFLFSLLQFLSAILPSLEAAPKNNTRLFREYIGA-DGNVRFSDVPVHPDVEFHFI 60
           MGSRIFFLFS L FL AILPS++A P  NTRLFREYIGA D NVRFSDVP+H DVEFHFI
Sbjct: 19  MGSRIFFLFSFLGFLFAILPSVKAEPLKNTRLFREYIGAEDKNVRFSDVPIHSDVEFHFI 78

Query: 61  LSFAIDYTGLSSPSATNGKFRVFWDEQNLSPSSVSSIKARHSNIKFAVSLGGDTVGKE-F 120
           LSFAIDYTG SSPS TNGKF+VFWDE+NL+PSS+SSIKA+HSN+KFA+SLGGDTVG   F
Sbjct: 79  LSFAIDYTGSSSPSPTNGKFKVFWDEKNLTPSSISSIKAKHSNVKFALSLGGDTVGDGIF 138

Query: 121 AYFKPKSVKSWVKNAFKSISRIVKEYHLDGVDIDYEHFKADPDTFTECIGQLLLRLKHHN 180
            +FKPKS+ SWV NAFKSIS IVKEY LDGVDIDYEHFKA PDTFTECIGQLL RLK  N
Sbjct: 139 VFFKPKSITSWVNNAFKSISDIVKEYDLDGVDIDYEHFKASPDTFTECIGQLLQRLKSQN 198

Query: 181 VISFASIAPFEDDTVQSHYLALWRKYGHLIDYVNFQFYSYDKGTTVSQFVNHFKTQSSNY 240
           +ISFASIAPF+DDTVQSHY+ALWRKYG LIDYVNFQFYSY KGTTVSQF+NHF TQSSNY
Sbjct: 199 IISFASIAPFDDDTVQSHYMALWRKYGDLIDYVNFQFYSYGKGTTVSQFLNHFDTQSSNY 258

Query: 241 QGGNVLASFATDGSGGLTPEKGFFKACSILQSRGELHGIFVWSADDSKKNNFRHEKQAQN 300
           +GG VLASFATDGSGGL PEKGFFKAC+ LQSRGELHGIFVWSADDSKKNNFR+E QAQN
Sbjct: 259 EGGKVLASFATDGSGGLKPEKGFFKACNTLQSRGELHGIFVWSADDSKKNNFRNEIQAQN 318

Query: 301 LLANAE 305
           LLANAE
Sbjct: 319 LLANAE 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146270.19.7e-174100.00chitinase 2-like [Momordica charantia][more]
XP_038902883.15.0e-14685.34chitinase 2-like [Benincasa hispida][more]
XP_022944087.14.7e-14483.99chitinase 2-like [Cucurbita moschata][more]
KAG6570744.14.7e-14483.99hypothetical protein SDJN03_29659, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022986910.16.2e-14484.31chitinase 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SLP41.8e-10662.37Chitinase 1 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1[more]
Q7M4431.3e-10164.23Chitinase 2 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1[more]
P396572.2e-1931.02RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CWU04.7e-174100.00chitinase 2-like OS=Momordica charantia OX=3673 GN=LOC111015520 PE=3 SV=1[more]
A0A6J1FUT92.3e-14483.99chitinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111448631 PE=3 SV=1[more]
A0A6J1JFC63.0e-14484.31chitinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484508 PE=3 SV=1[more]
A0A5D3CQZ25.1e-14483.99Chitinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G0097... [more]
A0A1S3CLR65.1e-14483.99chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103501894 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000677Chitinase-likePRINTSPR005512SGLOBULINcoord: 76..91
score: 26.56
coord: 93..111
score: 36.84
coord: 263..282
score: 35.0
coord: 30..43
score: 45.24
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 89..226
e-value: 5.8E-16
score: 59.1
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 31..304
score: 26.616299
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..303
e-value: 1.1E-84
score: 286.3
NoneNo IPR availablePANTHERPTHR46476:SF3CHITINASE 2coord: 7..303
NoneNo IPR availablePANTHERPTHR46476CHITINASE 2-LIKEcoord: 7..303
NoneNo IPR availableCDDcd06544GH18_narbonincoord: 32..285
e-value: 1.64695E-128
score: 364.385
IPR001579Glycosyl hydrolases family 18 (GH18) active sitePROSITEPS01095GH18_1coord: 146..154
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 31..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g09160.1Moc02g09160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds