Moc02g08260 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g08260
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionprotein NRT1/ PTR FAMILY 8.1-like
Locationchr2: 5879019 .. 5881100 (+)
RNA-Seq ExpressionMoc02g08260
SyntenyMoc02g08260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAAGAAGACAGTATCTACACGCAAGATGGAACAGTGGACTACCGAGGAAATCCCGCTGTTAGGAGCCAAACGGGAACCTGGAGAGCCTGCCCCTATATTTTGGGTAATTTTCCTGTGCTGAAAAATTTGATTGATGAGTTTCCAAAATAGGGATTCAACTAATGATTCTGAATATCTGCTCATAACATAACATAAACTGGTGCTGAATCTTGAATAGGAAATGAGTTTTGTGAGAGATTGGCTTACTATGGAATGAGCTCCAACCTGGTGATTTATTTTACGAAACATCTGAATCAGCACAGTGCCACAGCCTCTAAAAATGTGTCAAATTGGAGTGGTACCTGTTACATTACCCCCTTGATTGGAGCCTTTCTGGCTGATGCCTATTTGGGTAGATATTACACCATTGCCAGCTTCTCCATCCTCTATGTTATTGTGAGTTCTGTATCTGTAAGAAATTTATGTAGAAGATAGAGATTTATTAGGATTAAGCTTGAAGTTTAGTGTAATACATATGCAGGGGATGACACTTTTGACGTTGTCAGCATCAGTCCCTGGCCTAAAGCCCACCTGCGTCGCGAAAGATGATTGTTATGCAACGAATGCTCAAAGTGCGGTGTGTTTTGTGGCATTGTACCTGATTGCATTGGGGACCGGTGGGATTAAGCCTTGTGTTTCGTCTTATGGAGCGGATCAGTTTGATGATGCTAATGAGACTGAGAAGAGGCACAAGAGCTCTTTCTTCAATTGGTTCTATCTTTCAATCAATGTTGGGGCTCTTATTGCAAGTTCTGTGCTGGTTTGGGTGCAAGATAATGTGAGTTGGGGGTGGGGATTTGGTATTCCAGCAGTGGCTATGGCGATTGCTGTGTTGAGTTTCTTTTCGGGCACTCGACTGTATCGGAACCAGAAACCTGGAGGAAGTCCTTTTACCCGCATAAGTCAGGTCCTGGTGTCATCTTTTAGAAAATACAAGGTCAAAGTACCAGAAAGCAAGTCTCTGTACGAGACTGCTGATTCTGAGTCTTCTATAGTAGGAAGCCGCAAACTCGACCACACCGATGATTTCAGGTTTGCTTTTAAGACCGTAAACCCTGTCTCAAAGCGCTTCGTAGTGTATCCATCTAGGCTGAAAAGGATAAAACTTCAATATCTAGACTTGCTACATCAAAAACTACATGTAAGAAACGAGTCATTATGTATCATCTTCTTTTTGTGCCAAATTCTGTTGTATTTTTCAGGTTCTTTGACAAGGCAGCAGTGGAGCTAGAATCAGATCAGATGTTGAAGGGCTCAGTAGACCCATGGAAGCTTTGCACTGTCACTCAAGTAGAGGAGCTAAAAGCCATCATAAGGTTGCTTCCAGTATGGGCCACCGGTATCGTGTTCGCTGCTGTGTATAGTCAAATGGGCAATTTATTTGTGTTGCAGGGTGACAGAATGAATATTCATCTTGGCCCCAACTTTGAAATCCCAGCTGCATCTCTCTCAATCTTTGACACACTGAGTGTGATCTTTTGGGTCCCGGTTTATGATCGAATCATAGTTCCAGTGGCTCGAAAATACACTGGCCACCCGAATGGCATAACTCAACTGCAGAGAATGGGCATTGGCCTGCTTATTTCAATTCTAGCTATGCTATCTGCAGCGATCTTAGAACTCGTGAGGCTCCGAGAAGTGAAAAGGCACAACTACTATGAACTCAAGCACATGCCCATGTCCATCTTCTGGCAAGTTCCTCAGTATTTTCTCATTGGTTGTGCAGAAGTATTTACTTTCATTGGCCAGTTGGAGTTCTTTTACGAGCAAGCTCCCGATGCCATGAGGAGTCTCGGCTCGGCTCTCTCGCTCACTACTGTTGCACTTGGAAACTACCTGAGCTCTCTACTTGTGACTATTGTGACCAATGTCAGCACTAAAAATGGGAAGCTTGGATGGATACCAGACAATTTGAACTACGGCCATATACATTATTTCTTCTTTCTCTTGGCGCTGTTGAGTGTCAAAAACTTGATTGCTTTTATTTTCATAGCCAAGTGGTACAAGTATAAGAGGCCCGTTGGAACCCTTCGCTGA

mRNA sequence

ATGGAGGAAGAAGACAGTATCTACACGCAAGATGGAACAGTGGACTACCGAGGAAATCCCGCTGTTAGGAGCCAAACGGGAACCTGGAGAGCCTGCCCCTATATTTTGGGAAATGAGTTTTGTGAGAGATTGGCTTACTATGGAATGAGCTCCAACCTGGTGATTTATTTTACGAAACATCTGAATCAGCACAGTGCCACAGCCTCTAAAAATGTGTCAAATTGGAGTGGTACCTGTTACATTACCCCCTTGATTGGAGCCTTTCTGGCTGATGCCTATTTGGGTAGATATTACACCATTGCCAGCTTCTCCATCCTCTATGTTATTGGGATGACACTTTTGACGTTGTCAGCATCAGTCCCTGGCCTAAAGCCCACCTGCGTCGCGAAAGATGATTGTTATGCAACGAATGCTCAAAGTGCGGTGTGTTTTGTGGCATTGTACCTGATTGCATTGGGGACCGGTGGGATTAAGCCTTGTGTTTCGTCTTATGGAGCGGATCAGTTTGATGATGCTAATGAGACTGAGAAGAGGCACAAGAGCTCTTTCTTCAATTGGTTCTATCTTTCAATCAATGTTGGGGCTCTTATTGCAAGTTCTGTGCTGGTTTGGGTGCAAGATAATGTGAGTTGGGGGTGGGGATTTGGTATTCCAGCAGTGGCTATGGCGATTGCTGTGTTGAGTTTCTTTTCGGGCACTCGACTGTATCGGAACCAGAAACCTGGAGGAAGTCCTTTTACCCGCATAAGTCAGGTCCTGGTGTCATCTTTTAGAAAATACAAGGTCAAAGTACCAGAAAGCAAGTCTCTGTACGAGACTGCTGATTCTGAGTCTTCTATAGTAGGAAGCCGCAAACTCGACCACACCGATGATTTCAGGTTCTTTGACAAGGCAGCAGTGGAGCTAGAATCAGATCAGATGTTGAAGGGCTCAGTAGACCCATGGAAGCTTTGCACTGTCACTCAAGTAGAGGAGCTAAAAGCCATCATAAGGTTGCTTCCAGTATGGGCCACCGGTATCGTGTTCGCTGCTGTGTATAGTCAAATGGGCAATTTATTTGTGTTGCAGGGTGACAGAATGAATATTCATCTTGGCCCCAACTTTGAAATCCCAGCTGCATCTCTCTCAATCTTTGACACACTGAGTGTGATCTTTTGGGTCCCGGTTTATGATCGAATCATAGTTCCAGTGGCTCGAAAATACACTGGCCACCCGAATGGCATAACTCAACTGCAGAGAATGGGCATTGGCCTGCTTATTTCAATTCTAGCTATGCTATCTGCAGCGATCTTAGAACTCGTGAGGCTCCGAGAAGTGAAAAGGCACAACTACTATGAACTCAAGCACATGCCCATGTCCATCTTCTGGCAAGTTCCTCAGTATTTTCTCATTGGTTGTGCAGAAGTATTTACTTTCATTGGCCAGTTGGAGTTCTTTTACGAGCAAGCTCCCGATGCCATGAGGAGTCTCGGCTCGGCTCTCTCGCTCACTACTGTTGCACTTGGAAACTACCTGAGCTCTCTACTTGTGACTATTGTGACCAATGTCAGCACTAAAAATGGGAAGCTTGGATGGATACCAGACAATTTGAACTACGGCCATATACATTATTTCTTCTTTCTCTTGGCGCTGTTGAGTGTCAAAAACTTGATTGCTTTTATTTTCATAGCCAAGTGGTACAAGTATAAGAGGCCCGTTGGAACCCTTCGCTGA

Coding sequence (CDS)

ATGGAGGAAGAAGACAGTATCTACACGCAAGATGGAACAGTGGACTACCGAGGAAATCCCGCTGTTAGGAGCCAAACGGGAACCTGGAGAGCCTGCCCCTATATTTTGGGAAATGAGTTTTGTGAGAGATTGGCTTACTATGGAATGAGCTCCAACCTGGTGATTTATTTTACGAAACATCTGAATCAGCACAGTGCCACAGCCTCTAAAAATGTGTCAAATTGGAGTGGTACCTGTTACATTACCCCCTTGATTGGAGCCTTTCTGGCTGATGCCTATTTGGGTAGATATTACACCATTGCCAGCTTCTCCATCCTCTATGTTATTGGGATGACACTTTTGACGTTGTCAGCATCAGTCCCTGGCCTAAAGCCCACCTGCGTCGCGAAAGATGATTGTTATGCAACGAATGCTCAAAGTGCGGTGTGTTTTGTGGCATTGTACCTGATTGCATTGGGGACCGGTGGGATTAAGCCTTGTGTTTCGTCTTATGGAGCGGATCAGTTTGATGATGCTAATGAGACTGAGAAGAGGCACAAGAGCTCTTTCTTCAATTGGTTCTATCTTTCAATCAATGTTGGGGCTCTTATTGCAAGTTCTGTGCTGGTTTGGGTGCAAGATAATGTGAGTTGGGGGTGGGGATTTGGTATTCCAGCAGTGGCTATGGCGATTGCTGTGTTGAGTTTCTTTTCGGGCACTCGACTGTATCGGAACCAGAAACCTGGAGGAAGTCCTTTTACCCGCATAAGTCAGGTCCTGGTGTCATCTTTTAGAAAATACAAGGTCAAAGTACCAGAAAGCAAGTCTCTGTACGAGACTGCTGATTCTGAGTCTTCTATAGTAGGAAGCCGCAAACTCGACCACACCGATGATTTCAGGTTCTTTGACAAGGCAGCAGTGGAGCTAGAATCAGATCAGATGTTGAAGGGCTCAGTAGACCCATGGAAGCTTTGCACTGTCACTCAAGTAGAGGAGCTAAAAGCCATCATAAGGTTGCTTCCAGTATGGGCCACCGGTATCGTGTTCGCTGCTGTGTATAGTCAAATGGGCAATTTATTTGTGTTGCAGGGTGACAGAATGAATATTCATCTTGGCCCCAACTTTGAAATCCCAGCTGCATCTCTCTCAATCTTTGACACACTGAGTGTGATCTTTTGGGTCCCGGTTTATGATCGAATCATAGTTCCAGTGGCTCGAAAATACACTGGCCACCCGAATGGCATAACTCAACTGCAGAGAATGGGCATTGGCCTGCTTATTTCAATTCTAGCTATGCTATCTGCAGCGATCTTAGAACTCGTGAGGCTCCGAGAAGTGAAAAGGCACAACTACTATGAACTCAAGCACATGCCCATGTCCATCTTCTGGCAAGTTCCTCAGTATTTTCTCATTGGTTGTGCAGAAGTATTTACTTTCATTGGCCAGTTGGAGTTCTTTTACGAGCAAGCTCCCGATGCCATGAGGAGTCTCGGCTCGGCTCTCTCGCTCACTACTGTTGCACTTGGAAACTACCTGAGCTCTCTACTTGTGACTATTGTGACCAATGTCAGCACTAAAAATGGGAAGCTTGGATGGATACCAGACAATTTGAACTACGGCCATATACATTATTTCTTCTTTCTCTTGGCGCTGTTGAGTGTCAAAAACTTGATTGCTTTTATTTTCATAGCCAAGTGGTACAAGTATAAGAGGCCCGTTGGAACCCTTCGCTGA

Protein sequence

MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKHLNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASVPGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHKSSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQKPGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAVELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRMNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLISILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFFLLALLSVKNLIAFIFIAKWYKYKRPVGTLR
Homology
BLAST of Moc02g08260 vs. NCBI nr
Match: XP_022148338.1 (protein NRT1/ PTR FAMILY 8.1-like [Momordica charantia])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH
Sbjct: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV
Sbjct: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK
Sbjct: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM
Sbjct: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI
Sbjct: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LLALLSVKNLIAFIFIAKWYKYKRPVGTLR
Sbjct: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 570

BLAST of Moc02g08260 vs. NCBI nr
Match: XP_038901014.1 (protein NRT1/ PTR FAMILY 8.1-like [Benincasa hispida])

HSP 1 Score: 1063.9 bits (2750), Expect = 4.8e-307
Identity = 519/570 (91.05%), Postives = 549/570 (96.32%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTVDYRG+PAVR+QTGTWRACPYILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVDYRGDPAVRTQTGTWRACPYILGNEFCERLAYYGMSSNLVLYFKNH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATAS+N +NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YV GMTLLTLSASV
Sbjct: 61  LNQHSATASQNANNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVFGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDC+AT AQSA+CF ALYLIALGTGGIKPCVSSYGADQFDDANETEK+HK
Sbjct: 121 PGLKPTCVAKDDCHATTAQSALCFSALYLIALGTGGIKPCVSSYGADQFDDANETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQ+NVSWGWGFGIPA+AMAIAV+SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAIAMAIAVVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QVLV+SFRKYK+KVPESK+LYET DSESSIVGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVLVASFRKYKLKVPESKALYETTDSESSIVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQ LKGSV+PWKLCTVTQVEELKAIIRLLPVWATGI FAAVYSQM  LFVLQGD+M
Sbjct: 301 ELESDQTLKGSVNPWKLCTVTQVEELKAIIRLLPVWATGITFAAVYSQMSTLFVLQGDKM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GP FEIPAASLSIFDTLSVIFWVP+YDR+IVPVARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DPHIGPKFEIPAASLSIFDTLSVIFWVPIYDRVIVPVARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SILAMLSAAILELVRLREVK HNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SILAMLSAAILELVRLREVKMHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTT+ALGNYLSSLL+TIVT V TKNG+LGWIPDNLNYGH+HYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTIALGNYLSSLLMTIVTKVGTKNGRLGWIPDNLNYGHVHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LL ++SVKNLIAF+FIAKWYKYKRP+GTLR
Sbjct: 541 LLGIMSVKNLIAFLFIAKWYKYKRPIGTLR 570

BLAST of Moc02g08260 vs. NCBI nr
Match: XP_008464060.1 (PREDICTED: protein NRT1/ PTR FAMILY 8.1-like [Cucumis melo] >KAA0061853.1 protein NRT1/ PTR FAMILY 8.1-like [Cucumis melo var. makuwa] >TYK15403.1 protein NRT1/ PTR FAMILY 8.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1060.4 bits (2741), Expect = 5.3e-306
Identity = 518/570 (90.88%), Postives = 551/570 (96.67%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTV+YRG+PAVR+QTGTWRACPYILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVNYRGDPAVRTQTGTWRACPYILGNEFCERLAYYGMSSNLVLYFKHH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQ SATASKNV+NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YV GMTLLTLSASV
Sbjct: 61  LNQDSATASKNVNNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVFGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCV+KDDC+AT AQSA+CF+ALYLIALGTGGIKPCVSSYGADQFDDANETEK+HK
Sbjct: 121 PGLKPTCVSKDDCHATTAQSALCFLALYLIALGTGGIKPCVSSYGADQFDDANETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSIN+GALIASSVLVWVQ+NVSWGWGFGIPA+AMAIA++SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINIGALIASSVLVWVQENVSWGWGFGIPAIAMAIAIVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V++FRKY VKVPESK+LYETADSESSIVGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVATFRKYNVKVPESKALYETADSESSIVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGI FAAVYSQM +LFVLQGDRM
Sbjct: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGITFAAVYSQMSSLFVLQGDRM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GP FEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DPHIGPTFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SILAMLSAAILELVRLREV+RH+YYELKHMPMSIFWQVPQYFLIGCAEVFT IGQLEFFY
Sbjct: 421 SILAMLSAAILELVRLREVRRHDYYELKHMPMSIFWQVPQYFLIGCAEVFTLIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTT+ALGNYLSSLLVTIV   STK G+LGWIPDNLNYGH+HYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTIALGNYLSSLLVTIVNKASTKGGRLGWIPDNLNYGHVHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LLA+LSVKNLIAF+FIAKWYKYKRP+GTLR
Sbjct: 541 LLAILSVKNLIAFLFIAKWYKYKRPIGTLR 570

BLAST of Moc02g08260 vs. NCBI nr
Match: KAG6570671.1 (Protein NRT1/ PTR FAMILY 8.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.0e-306
Identity = 517/570 (90.70%), Postives = 555/570 (97.37%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTVDYRG+PAVR++TGTW+ACP+ILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVDYRGDPAVRTKTGTWKACPFILGNEFCERLAYYGMSSNLVLYFKNH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATASKN +NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YVIGMTLLTLSASV
Sbjct: 61  LNQHSATASKNANNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVIGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDC+AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDA+ETEK+HK
Sbjct: 121 PGLKPTCVAKDDCHATAAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDADETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQ+NVSWGWGFGIPA+AMAIAV+SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAMAMAIAVVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V+SFRKY+VKVPE+K+LYETAD+ES++VGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVASFRKYEVKVPENKALYETADNESAVVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESD+MLKG+V+PWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG LFVLQGD+M
Sbjct: 301 ELESDEMLKGTVNPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGTLFVLQGDKM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GPNFEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DAHIGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SI+AMLSAAILELVRLREV+RHNYYEL HMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SIIAMLSAAILELVRLREVRRHNYYELDHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTTVALGNY+SSLLVTIVT VSTK+G  GWIPDNLNYGHIHYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTVALGNYMSSLLVTIVTKVSTKDGSPGWIPDNLNYGHIHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LL +LSVKNLIA++FIAKWYKYKRP+GTLR
Sbjct: 541 LLVILSVKNLIAYLFIAKWYKYKRPLGTLR 570

BLAST of Moc02g08260 vs. NCBI nr
Match: XP_022986822.1 (protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima])

HSP 1 Score: 1059.3 bits (2738), Expect = 1.2e-305
Identity = 518/570 (90.88%), Postives = 553/570 (97.02%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTVDYRG+PAVR++TGTW+ACP+ILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVDYRGDPAVRTKTGTWKACPFILGNEFCERLAYYGMSSNLVLYFKNH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATASKN +NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YVIGMTLLTLSASV
Sbjct: 61  LNQHSATASKNANNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVIGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDC+AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDA+ETEK+HK
Sbjct: 121 PGLKPTCVAKDDCHATAAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDADETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQ+NVSWGWGFGIPA+AMAIAV+SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAMAMAIAVVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V+SFRKY+VKVPE+K+LYET D+ES++VGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVASFRKYEVKVPENKALYETTDNESAVVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSV+PWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG LFVLQGD+M
Sbjct: 301 ELESDQMLKGSVNPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGTLFVLQGDKM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GPNFEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DAHIGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SI+AMLSAAILELVRLREV+RHNYYEL HMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SIIAMLSAAILELVRLREVRRHNYYELDHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVT  STK+G  GWIPDNLNYGHIHYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTKFSTKDGSPGWIPDNLNYGHIHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LL +LSVKNLIA++FIAKWYKYKRP+GTLR
Sbjct: 541 LLVILSVKNLIAYLFIAKWYKYKRPLGTLR 570

BLAST of Moc02g08260 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 4.0e-232
Identity = 396/568 (69.72%), Postives = 473/568 (83.27%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           MEE+D +YTQDGTVD   NPA + +TG W+AC +ILGNE CERLAYYGM +NLV Y    
Sbjct: 1   MEEKD-VYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESR 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQ +ATA+ NV+NWSGTCYITPLIGAF+ADAYLGRY+TIA+F  +YV GMTLLTLSASV
Sbjct: 61  LNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKP     D C+  ++Q+AV FVALY+IALGTGGIKPCVSS+GADQFD+ +E EK  K
Sbjct: 121 PGLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFY SINVGALIA++VLVW+Q NV WGWGFG+P VAM IAV  FF G+R YR Q+
Sbjct: 181 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAA 300
           PGGSP TRI QV+V++FRK  VKVPE KS L+ETAD ES+I GSRKL HTD+ +FFDKAA
Sbjct: 241 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAA 300

Query: 301 VELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDR 360
           VE +SD +  G V+PW+LC+VTQVEELK+II LLPVWATGIVFA VYSQM  +FVLQG+ 
Sbjct: 301 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 360

Query: 361 MNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLL 420
           M+ H+G NFEIP+ASLS+FDT+SV+FW PVYD+ I+P+ARK+T +  G TQLQRMGIGL+
Sbjct: 361 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 420

Query: 421 ISILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 480
           +SI AM++A +LE+VRL  VK HN Y+ K + MSIFWQ+PQY LIGCAEVFTFIGQLEFF
Sbjct: 421 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 480

Query: 481 YEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFF 540
           Y+QAPDAMRSL SALSLTTVALGNYLS++LVT+V  ++ KNGK GWIPDNLN GH+ YFF
Sbjct: 481 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFF 540

Query: 541 FLLALLSVKNLIAFIFIAKWYKYKRPVG 568
           +LLA LS  N + +++I+K YKYK+ VG
Sbjct: 541 YLLATLSFLNFLVYLWISKRYKYKKAVG 567

BLAST of Moc02g08260 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 1.9e-229
Identity = 386/568 (67.96%), Postives = 466/568 (82.04%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           ME++  IYT+DGT+D    PA +++TGTW+AC +ILG E CERLAYYGMS+NL+ Y  K 
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           +N  + +ASK+VSNWSGTCY TPLIGAF+ADAYLGRY+TIASF ++Y+ GMTLLT+SASV
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGL PTC + + C+AT  Q+A+ F+ALYLIALGTGGIKPCVSS+GADQFDD +E EK  K
Sbjct: 121 PGLTPTC-SGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFY  INVGA+IASSVLVW+Q NV WGWG G+P VAMAIAV+ FF+G+  YR QK
Sbjct: 181 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAA 300
           PGGSP TR+ QV+V+S RK KVK+PE +S LYE  D+ESSI+GSRKL+HT    FFDKAA
Sbjct: 241 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAA 300

Query: 301 VELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDR 360
           VE ESD         WKLCTVTQVEELKA+IRLLP+WATGIVFA+VYSQMG +FVLQG+ 
Sbjct: 301 VETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNT 360

Query: 361 MNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLL 420
           ++ H+GPNF+IP+ASLS+FDTLSV+FW PVYD++IVP ARKYTGH  G TQLQR+GIGL+
Sbjct: 361 LDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLV 420

Query: 421 ISILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 480
           ISI +M+SA ILE+ RL  V+ HN Y  + +PM+IFWQVPQYFL+GCAEVFTFIGQLEFF
Sbjct: 421 ISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFF 480

Query: 481 YEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFF 540
           Y+QAPDAMRSL SALSLT +A GNYLS+ LVT+VT V+   G+ GWI  NLN GH+ YFF
Sbjct: 481 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFF 540

Query: 541 FLLALLSVKNLIAFIFIAKWYKYKRPVG 568
           +LLA LS  N + +++IAKWY YK+  G
Sbjct: 541 WLLAGLSFLNFLVYLWIAKWYTYKKTTG 567

BLAST of Moc02g08260 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 3.0e-195
Identity = 340/565 (60.18%), Postives = 431/565 (76.28%), Query Frame = 0

Query: 3   EEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKHLN 62
           +E  +Y +DG+VD+ GNP ++ +TG W+ACP+ILGNE CERLAYYG++ NL+ Y T  L+
Sbjct: 19  QEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLH 78

Query: 63  QHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASVPG 122
           Q + +A+ NV+ W GTCY+TPLIGA LADAY GRY+TIA FS +Y IGM+ LTLSASVP 
Sbjct: 79  QGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPA 138

Query: 123 LKPTCVAKDDC-YATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHKS 182
           LKP     D C  AT AQ A+ F  LYLIALGTGGIKPCVSS+GADQFDD +  E+  K+
Sbjct: 139 LKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKA 198

Query: 183 SFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQKP 242
           SFFNWFY SIN+GAL++SS+LVW+Q+N  WG GFGIP V M +A+ SFF GT LYR QKP
Sbjct: 199 SFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKP 258

Query: 243 GGSPFTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAAV 302
           GGSP TRISQV+V+SFRK  VKVPE  + LYET D  S+I GSRK++HTDD ++ DKAAV
Sbjct: 259 GGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAV 318

Query: 303 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 362
             E +       + W+LCTVTQVEELK +IR+ P+WA+GI+F+AVY+QM  +FV QG  M
Sbjct: 319 ISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAM 378

Query: 363 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 422
           N  +G +F++P A+L  FDT SVI WVP+YDR IVP+ARK+TG   G T++QRMGIGL +
Sbjct: 379 NCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFV 438

Query: 423 SILAMLSAAILELVRLREVKRHNYYEL-KHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 482
           S+L M +AAI+E++RL         E    +P+S+ WQ+PQYF++G AEVF FIGQLEFF
Sbjct: 439 SVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFF 498

Query: 483 YEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFF 542
           Y+Q+PDAMRSL SAL+L T ALGNYLSSL++T+VT  +T+NG+ GWI DNLN GH+ YFF
Sbjct: 499 YDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFF 558

Query: 543 FLLALLSVKNLIAFIFIAKWYKYKR 565
           +LLA LS+ N+  + F A  YK K+
Sbjct: 559 WLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Moc02g08260 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 1.7e-177
Identity = 312/560 (55.71%), Postives = 411/560 (73.39%), Query Frame = 0

Query: 10  QDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKHLNQHSATAS 69
           +DG++D  GNP  + +TG W+ACP+ILGNE CERLAYYG++ NL+ Y+T  L++ + +A+
Sbjct: 37  EDGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAA 96

Query: 70  KNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASVPGLKPTC-- 129
            +V  W GTCYITPLIGA +AD+Y GRY+TIASFS +Y IGM LLTLSAS+P LKP    
Sbjct: 97  SDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACA 156

Query: 130 -VAKDDCY-ATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHKSSFFN 189
            VA   C  AT  Q AV F  LYLIALGTGGIKPCVSS+GADQFDD +  E+  K+SFFN
Sbjct: 157 GVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFN 216

Query: 190 WFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQKPGGSP 249
           WFY SIN+G+ I+S++LVWVQ+NV WG GF IP V M +++ SFF GT LYR QKPGGSP
Sbjct: 217 WFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSP 276

Query: 250 FTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAAVELES 309
            TR+ QVLV+++RK K+ +PE  S LYET +  S I GSRK+ HTD ++F DKAAV  E 
Sbjct: 277 ITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEY 336

Query: 310 DQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRMNIHL 369
           +       +PWKLCTVTQVEE+K +IR+ P+WA+GIV++ +YSQ+  LFV QG  MN  +
Sbjct: 337 ESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMN-RI 396

Query: 370 GPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLISILA 429
             +FEIP AS  +FDTL V+  +P+YDR +VP  R++TG P G+T LQRMGIGL +S+L+
Sbjct: 397 IRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLS 456

Query: 430 MLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYEQAP 489
           + +AAI+E VRL+  +         + MSIFWQ+PQY L+G AEVF FIG++EFFY+++P
Sbjct: 457 IAAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESP 516

Query: 490 DAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFFLLAL 549
           DAMRS+ SAL+L   A+G+YLSSL++T+V   +   GK GW+PD+LN GH+ YFF+LL  
Sbjct: 517 DAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLVS 576

Query: 550 LSVKNLIAFIFIAKWYKYKR 565
           L + N+  +  I   +  K+
Sbjct: 577 LGLVNIPVYALICVKHTKKK 588

BLAST of Moc02g08260 vs. ExPASy Swiss-Prot
Match: Q84WG0 (Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2)

HSP 1 Score: 570.1 bits (1468), Expect = 2.8e-161
Identity = 296/567 (52.20%), Postives = 393/567 (69.31%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           ++EE  +Y +DG++D  GNP ++  TG W+ACP+I  NE CERLAYYG++ NL+ YFT  
Sbjct: 18  IQEEVKLYAEDGSIDIHGNPPLKQTTGNWKACPFIFANECCERLAYYGIAKNLITYFTNE 77

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           L++ + +A+++V  W GTCYITPLIGA +ADAY GRY+TIA FS +Y  GM  LTLSASV
Sbjct: 78  LHETNVSAARHVMTWQGTCYITPLIGALIADAYWGRYWTIACFSAIYFTGMVALTLSASV 137

Query: 121 PGLKPT-CVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRH 180
           PGLKP  C+      AT  QS V F  LYLIALGTGGIKPCVSS+GADQFD  + +E+  
Sbjct: 138 PGLKPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCVSSFGADQFDKTDPSERVR 197

Query: 181 KSSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQ 240
           K+SFFNWFY +IN+GA ++S+VLVW+Q+N  W  GF IP V M +A +SFF GT LYR Q
Sbjct: 198 KASFFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVFMGLATMSFFFGTPLYRFQ 257

Query: 241 KPGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAA 300
           KP GSP T + QVLV+++RK  +KVPE                    D TD         
Sbjct: 258 KPRGSPITSVCQVLVAAYRKSNLKVPE--------------------DSTD--------- 317

Query: 301 VELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDR 360
              E D     + +PWKLCTVTQVEE+K ++RL+P+WA+GI+F+ ++SQ+  LFV QG  
Sbjct: 318 ---EGD----ANTNPWKLCTVTQVEEVKILLRLVPIWASGIIFSVLHSQIYTLFVQQGRC 377

Query: 361 MNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLL 420
           M   +G  FEIP A+L +FDT SV+  VP+YDR+IVP+ R++TG   G T+LQRMGIGL 
Sbjct: 378 MKRTIG-LFEIPPATLGMFDTASVLISVPIYDRVIVPLVRRFTGLAKGFTELQRMGIGLF 437

Query: 421 ISILAMLSAAILELVRLREVKRHNYYELKH-MPMSIFWQVPQYFLIGCAEVFTFIGQLEF 480
           +S+L++  AAI+E VRL+  +  +  E    +P++IFWQ+PQYFL+G A VF F+G++EF
Sbjct: 438 VSVLSLTFAAIVETVRLQLARDLDLVESGDIVPLNIFWQIPQYFLMGTAGVFFFVGRIEF 497

Query: 481 FYEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIP-DNLNYGHIHY 540
           FYEQ+PD+MRSL SA +L T  LGNYLSSL++T+V  +S   GK  WIP DN+N GH+ Y
Sbjct: 498 FYEQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYLS---GKDCWIPSDNINNGHLDY 544

Query: 541 FFFLLALLSVKNLIAFIFIAKWYKYKR 565
           FF+LL  L   N+  F+F +  Y + +
Sbjct: 558 FFWLLVSLGSVNIPVFVFFSVKYTHMK 544

BLAST of Moc02g08260 vs. ExPASy TrEMBL
Match: A0A6J1D3U5 (protein NRT1/ PTR FAMILY 8.1-like OS=Momordica charantia OX=3673 GN=LOC111017019 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH
Sbjct: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV
Sbjct: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK
Sbjct: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM
Sbjct: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI
Sbjct: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LLALLSVKNLIAFIFIAKWYKYKRPVGTLR
Sbjct: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 570

BLAST of Moc02g08260 vs. ExPASy TrEMBL
Match: A0A5A7V118 (Protein NRT1/ PTR FAMILY 8.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00460 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.6e-306
Identity = 518/570 (90.88%), Postives = 551/570 (96.67%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTV+YRG+PAVR+QTGTWRACPYILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVNYRGDPAVRTQTGTWRACPYILGNEFCERLAYYGMSSNLVLYFKHH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQ SATASKNV+NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YV GMTLLTLSASV
Sbjct: 61  LNQDSATASKNVNNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVFGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCV+KDDC+AT AQSA+CF+ALYLIALGTGGIKPCVSSYGADQFDDANETEK+HK
Sbjct: 121 PGLKPTCVSKDDCHATTAQSALCFLALYLIALGTGGIKPCVSSYGADQFDDANETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSIN+GALIASSVLVWVQ+NVSWGWGFGIPA+AMAIA++SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINIGALIASSVLVWVQENVSWGWGFGIPAIAMAIAIVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V++FRKY VKVPESK+LYETADSESSIVGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVATFRKYNVKVPESKALYETADSESSIVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGI FAAVYSQM +LFVLQGDRM
Sbjct: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGITFAAVYSQMSSLFVLQGDRM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GP FEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DPHIGPTFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SILAMLSAAILELVRLREV+RH+YYELKHMPMSIFWQVPQYFLIGCAEVFT IGQLEFFY
Sbjct: 421 SILAMLSAAILELVRLREVRRHDYYELKHMPMSIFWQVPQYFLIGCAEVFTLIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTT+ALGNYLSSLLVTIV   STK G+LGWIPDNLNYGH+HYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTIALGNYLSSLLVTIVNKASTKGGRLGWIPDNLNYGHVHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LLA+LSVKNLIAF+FIAKWYKYKRP+GTLR
Sbjct: 541 LLAILSVKNLIAFLFIAKWYKYKRPIGTLR 570

BLAST of Moc02g08260 vs. ExPASy TrEMBL
Match: A0A1S3CL31 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucumis melo OX=3656 GN=LOC103502040 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.6e-306
Identity = 518/570 (90.88%), Postives = 551/570 (96.67%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTV+YRG+PAVR+QTGTWRACPYILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVNYRGDPAVRTQTGTWRACPYILGNEFCERLAYYGMSSNLVLYFKHH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQ SATASKNV+NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YV GMTLLTLSASV
Sbjct: 61  LNQDSATASKNVNNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVFGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCV+KDDC+AT AQSA+CF+ALYLIALGTGGIKPCVSSYGADQFDDANETEK+HK
Sbjct: 121 PGLKPTCVSKDDCHATTAQSALCFLALYLIALGTGGIKPCVSSYGADQFDDANETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSIN+GALIASSVLVWVQ+NVSWGWGFGIPA+AMAIA++SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINIGALIASSVLVWVQENVSWGWGFGIPAIAMAIAIVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V++FRKY VKVPESK+LYETADSESSIVGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVATFRKYNVKVPESKALYETADSESSIVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGI FAAVYSQM +LFVLQGDRM
Sbjct: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGITFAAVYSQMSSLFVLQGDRM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GP FEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DPHIGPTFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SILAMLSAAILELVRLREV+RH+YYELKHMPMSIFWQVPQYFLIGCAEVFT IGQLEFFY
Sbjct: 421 SILAMLSAAILELVRLREVRRHDYYELKHMPMSIFWQVPQYFLIGCAEVFTLIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTT+ALGNYLSSLLVTIV   STK G+LGWIPDNLNYGH+HYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTIALGNYLSSLLVTIVNKASTKGGRLGWIPDNLNYGHVHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LLA+LSVKNLIAF+FIAKWYKYKRP+GTLR
Sbjct: 541 LLAILSVKNLIAFLFIAKWYKYKRPIGTLR 570

BLAST of Moc02g08260 vs. ExPASy TrEMBL
Match: A0A6J1J8M5 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111484451 PE=3 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 5.7e-306
Identity = 518/570 (90.88%), Postives = 553/570 (97.02%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTVDYRG+PAVR++TGTW+ACP+ILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVDYRGDPAVRTKTGTWKACPFILGNEFCERLAYYGMSSNLVLYFKNH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATASKN +NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YVIGMTLLTLSASV
Sbjct: 61  LNQHSATASKNANNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVIGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDC+AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDA+ETEK+HK
Sbjct: 121 PGLKPTCVAKDDCHATAAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDADETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQ+NVSWGWGFGIPA+AMAIAV+SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAMAMAIAVVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V+SFRKY+VKVPE+K+LYET D+ES++VGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVASFRKYEVKVPENKALYETTDNESAVVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESDQMLKGSV+PWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG LFVLQGD+M
Sbjct: 301 ELESDQMLKGSVNPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGTLFVLQGDKM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GPNFEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DAHIGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SI+AMLSAAILELVRLREV+RHNYYEL HMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SIIAMLSAAILELVRLREVRRHNYYELDHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVT  STK+G  GWIPDNLNYGHIHYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTKFSTKDGSPGWIPDNLNYGHIHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LL +LSVKNLIA++FIAKWYKYKRP+GTLR
Sbjct: 541 LLVILSVKNLIAYLFIAKWYKYKRPLGTLR 570

BLAST of Moc02g08260 vs. ExPASy TrEMBL
Match: A0A6J1FV98 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111448422 PE=3 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 7.4e-306
Identity = 517/570 (90.70%), Postives = 555/570 (97.37%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           M EEDSIYTQDGTVDYRG+PAVR++TGTW+ACP+ILGNEFCERLAYYGMSSNLV+YF  H
Sbjct: 1   MGEEDSIYTQDGTVDYRGDPAVRTKTGTWKACPFILGNEFCERLAYYGMSSNLVLYFKNH 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQHSATASKN +NWSGTCYITPLIGAFLADAYLGRY TIA+FSI+YVIGMTLLTLSASV
Sbjct: 61  LNQHSATASKNANNWSGTCYITPLIGAFLADAYLGRYRTIAAFSIVYVIGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKPTCVAKDDC+AT AQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDA+ETEK+HK
Sbjct: 121 PGLKPTCVAKDDCHATAAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDADETEKKHK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFYLSINVGALIASSVLVWVQ+NVSWGWGFGIPA+AMAIAV+SFFSGTRLYRNQK
Sbjct: 181 SSFFNWFYLSINVGALIASSVLVWVQENVSWGWGFGIPAMAMAIAVVSFFSGTRLYRNQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAAV 300
           PGGSPFTRI QV+V+SFRKY+VKVPE+K+LYETAD+ES++VGSRKLDHTDDFRFFDKAAV
Sbjct: 241 PGGSPFTRICQVVVASFRKYEVKVPENKALYETADNESAVVGSRKLDHTDDFRFFDKAAV 300

Query: 301 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 360
           ELESD+MLKG+V+PWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMG LFVLQGD+M
Sbjct: 301 ELESDEMLKGTVNPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGTLFVLQGDKM 360

Query: 361 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 420
           + H+GPNFEIPAASLSIFDTLSVIFWVPVYDRIIVP+ARKYTGHPNGITQLQRMGIGL I
Sbjct: 361 DAHIGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPMARKYTGHPNGITQLQRMGIGLFI 420

Query: 421 SILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480
           SI+AMLSAAILELVRL+EV+RHNYYEL HMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY
Sbjct: 421 SIIAMLSAAILELVRLQEVRRHNYYELDHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 480

Query: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFF 540
           EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVT VSTK+G  GWIPDNLNYGHIHYFFF
Sbjct: 481 EQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTKVSTKDGSPGWIPDNLNYGHIHYFFF 540

Query: 541 LLALLSVKNLIAFIFIAKWYKYKRPVGTLR 571
           LL +LSVKNLIA++FIAKWYKYKRP+GTLR
Sbjct: 541 LLVILSVKNLIAYLFIAKWYKYKRPLGTLR 570

BLAST of Moc02g08260 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 805.4 bits (2079), Expect = 2.9e-233
Identity = 396/568 (69.72%), Postives = 473/568 (83.27%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           MEE+D +YTQDGTVD   NPA + +TG W+AC +ILGNE CERLAYYGM +NLV Y    
Sbjct: 1   MEEKD-VYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESR 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           LNQ +ATA+ NV+NWSGTCYITPLIGAF+ADAYLGRY+TIA+F  +YV GMTLLTLSASV
Sbjct: 61  LNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGLKP     D C+  ++Q+AV FVALY+IALGTGGIKPCVSS+GADQFD+ +E EK  K
Sbjct: 121 PGLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFY SINVGALIA++VLVW+Q NV WGWGFG+P VAM IAV  FF G+R YR Q+
Sbjct: 181 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAA 300
           PGGSP TRI QV+V++FRK  VKVPE KS L+ETAD ES+I GSRKL HTD+ +FFDKAA
Sbjct: 241 PGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAA 300

Query: 301 VELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDR 360
           VE +SD +  G V+PW+LC+VTQVEELK+II LLPVWATGIVFA VYSQM  +FVLQG+ 
Sbjct: 301 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 360

Query: 361 MNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLL 420
           M+ H+G NFEIP+ASLS+FDT+SV+FW PVYD+ I+P+ARK+T +  G TQLQRMGIGL+
Sbjct: 361 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 420

Query: 421 ISILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 480
           +SI AM++A +LE+VRL  VK HN Y+ K + MSIFWQ+PQY LIGCAEVFTFIGQLEFF
Sbjct: 421 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 480

Query: 481 YEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFF 540
           Y+QAPDAMRSL SALSLTTVALGNYLS++LVT+V  ++ KNGK GWIPDNLN GH+ YFF
Sbjct: 481 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFF 540

Query: 541 FLLALLSVKNLIAFIFIAKWYKYKRPVG 568
           +LLA LS  N + +++I+K YKYK+ VG
Sbjct: 541 YLLATLSFLNFLVYLWISKRYKYKKAVG 567

BLAST of Moc02g08260 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 796.6 bits (2056), Expect = 1.3e-230
Identity = 386/568 (67.96%), Postives = 466/568 (82.04%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           ME++  IYT+DGT+D    PA +++TGTW+AC +ILG E CERLAYYGMS+NL+ Y  K 
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           +N  + +ASK+VSNWSGTCY TPLIGAF+ADAYLGRY+TIASF ++Y+ GMTLLT+SASV
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 121 PGLKPTCVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHK 180
           PGL PTC + + C+AT  Q+A+ F+ALYLIALGTGGIKPCVSS+GADQFDD +E EK  K
Sbjct: 121 PGLTPTC-SGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 180

Query: 181 SSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQK 240
           SSFFNWFY  INVGA+IASSVLVW+Q NV WGWG G+P VAMAIAV+ FF+G+  YR QK
Sbjct: 181 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 240

Query: 241 PGGSPFTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAA 300
           PGGSP TR+ QV+V+S RK KVK+PE +S LYE  D+ESSI+GSRKL+HT    FFDKAA
Sbjct: 241 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAA 300

Query: 301 VELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDR 360
           VE ESD         WKLCTVTQVEELKA+IRLLP+WATGIVFA+VYSQMG +FVLQG+ 
Sbjct: 301 VETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNT 360

Query: 361 MNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLL 420
           ++ H+GPNF+IP+ASLS+FDTLSV+FW PVYD++IVP ARKYTGH  G TQLQR+GIGL+
Sbjct: 361 LDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLV 420

Query: 421 ISILAMLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 480
           ISI +M+SA ILE+ RL  V+ HN Y  + +PM+IFWQVPQYFL+GCAEVFTFIGQLEFF
Sbjct: 421 ISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFF 480

Query: 481 YEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFF 540
           Y+QAPDAMRSL SALSLT +A GNYLS+ LVT+VT V+   G+ GWI  NLN GH+ YFF
Sbjct: 481 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFF 540

Query: 541 FLLALLSVKNLIAFIFIAKWYKYKRPVG 568
           +LLA LS  N + +++IAKWY YK+  G
Sbjct: 541 WLLAGLSFLNFLVYLWIAKWYTYKKTTG 567

BLAST of Moc02g08260 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 682.9 bits (1761), Expect = 2.1e-196
Identity = 340/565 (60.18%), Postives = 431/565 (76.28%), Query Frame = 0

Query: 3   EEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKHLN 62
           +E  +Y +DG+VD+ GNP ++ +TG W+ACP+ILGNE CERLAYYG++ NL+ Y T  L+
Sbjct: 19  QEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLH 78

Query: 63  QHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASVPG 122
           Q + +A+ NV+ W GTCY+TPLIGA LADAY GRY+TIA FS +Y IGM+ LTLSASVP 
Sbjct: 79  QGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPA 138

Query: 123 LKPTCVAKDDC-YATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHKS 182
           LKP     D C  AT AQ A+ F  LYLIALGTGGIKPCVSS+GADQFDD +  E+  K+
Sbjct: 139 LKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKA 198

Query: 183 SFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQKP 242
           SFFNWFY SIN+GAL++SS+LVW+Q+N  WG GFGIP V M +A+ SFF GT LYR QKP
Sbjct: 199 SFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKP 258

Query: 243 GGSPFTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAAV 302
           GGSP TRISQV+V+SFRK  VKVPE  + LYET D  S+I GSRK++HTDD ++ DKAAV
Sbjct: 259 GGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAV 318

Query: 303 ELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRM 362
             E +       + W+LCTVTQVEELK +IR+ P+WA+GI+F+AVY+QM  +FV QG  M
Sbjct: 319 ISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAM 378

Query: 363 NIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLI 422
           N  +G +F++P A+L  FDT SVI WVP+YDR IVP+ARK+TG   G T++QRMGIGL +
Sbjct: 379 NCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFV 438

Query: 423 SILAMLSAAILELVRLREVKRHNYYEL-KHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 482
           S+L M +AAI+E++RL         E    +P+S+ WQ+PQYF++G AEVF FIGQLEFF
Sbjct: 439 SVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFF 498

Query: 483 YEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFF 542
           Y+Q+PDAMRSL SAL+L T ALGNYLSSL++T+VT  +T+NG+ GWI DNLN GH+ YFF
Sbjct: 499 YDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFF 558

Query: 543 FLLALLSVKNLIAFIFIAKWYKYKR 565
           +LLA LS+ N+  + F A  YK K+
Sbjct: 559 WLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Moc02g08260 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 624.0 bits (1608), Expect = 1.2e-178
Identity = 312/560 (55.71%), Postives = 411/560 (73.39%), Query Frame = 0

Query: 10  QDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKHLNQHSATAS 69
           +DG++D  GNP  + +TG W+ACP+ILGNE CERLAYYG++ NL+ Y+T  L++ + +A+
Sbjct: 37  EDGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAA 96

Query: 70  KNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASVPGLKPTC-- 129
            +V  W GTCYITPLIGA +AD+Y GRY+TIASFS +Y IGM LLTLSAS+P LKP    
Sbjct: 97  SDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACA 156

Query: 130 -VAKDDCY-ATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRHKSSFFN 189
            VA   C  AT  Q AV F  LYLIALGTGGIKPCVSS+GADQFDD +  E+  K+SFFN
Sbjct: 157 GVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFN 216

Query: 190 WFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQKPGGSP 249
           WFY SIN+G+ I+S++LVWVQ+NV WG GF IP V M +++ SFF GT LYR QKPGGSP
Sbjct: 217 WFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSP 276

Query: 250 FTRISQVLVSSFRKYKVKVPESKS-LYETADSESSIVGSRKLDHTDDFRFFDKAAVELES 309
            TR+ QVLV+++RK K+ +PE  S LYET +  S I GSRK+ HTD ++F DKAAV  E 
Sbjct: 277 ITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEY 336

Query: 310 DQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDRMNIHL 369
           +       +PWKLCTVTQVEE+K +IR+ P+WA+GIV++ +YSQ+  LFV QG  MN  +
Sbjct: 337 ESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMN-RI 396

Query: 370 GPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLLISILA 429
             +FEIP AS  +FDTL V+  +P+YDR +VP  R++TG P G+T LQRMGIGL +S+L+
Sbjct: 397 IRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLS 456

Query: 430 MLSAAILELVRLREVKRHNYYELKHMPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYEQAP 489
           + +AAI+E VRL+  +         + MSIFWQ+PQY L+G AEVF FIG++EFFY+++P
Sbjct: 457 IAAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESP 516

Query: 490 DAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIPDNLNYGHIHYFFFLLAL 549
           DAMRS+ SAL+L   A+G+YLSSL++T+V   +   GK GW+PD+LN GH+ YFF+LL  
Sbjct: 517 DAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLVS 576

Query: 550 LSVKNLIAFIFIAKWYKYKR 565
           L + N+  +  I   +  K+
Sbjct: 577 LGLVNIPVYALICVKHTKKK 588

BLAST of Moc02g08260 vs. TAIR 10
Match: AT2G02020.1 (Major facilitator superfamily protein )

HSP 1 Score: 570.1 bits (1468), Expect = 2.0e-162
Identity = 296/567 (52.20%), Postives = 393/567 (69.31%), Query Frame = 0

Query: 1   MEEEDSIYTQDGTVDYRGNPAVRSQTGTWRACPYILGNEFCERLAYYGMSSNLVIYFTKH 60
           ++EE  +Y +DG++D  GNP ++  TG W+ACP+I  NE CERLAYYG++ NL+ YFT  
Sbjct: 18  IQEEVKLYAEDGSIDIHGNPPLKQTTGNWKACPFIFANECCERLAYYGIAKNLITYFTNE 77

Query: 61  LNQHSATASKNVSNWSGTCYITPLIGAFLADAYLGRYYTIASFSILYVIGMTLLTLSASV 120
           L++ + +A+++V  W GTCYITPLIGA +ADAY GRY+TIA FS +Y  GM  LTLSASV
Sbjct: 78  LHETNVSAARHVMTWQGTCYITPLIGALIADAYWGRYWTIACFSAIYFTGMVALTLSASV 137

Query: 121 PGLKPT-CVAKDDCYATNAQSAVCFVALYLIALGTGGIKPCVSSYGADQFDDANETEKRH 180
           PGLKP  C+      AT  QS V F  LYLIALGTGGIKPCVSS+GADQFD  + +E+  
Sbjct: 138 PGLKPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCVSSFGADQFDKTDPSERVR 197

Query: 181 KSSFFNWFYLSINVGALIASSVLVWVQDNVSWGWGFGIPAVAMAIAVLSFFSGTRLYRNQ 240
           K+SFFNWFY +IN+GA ++S+VLVW+Q+N  W  GF IP V M +A +SFF GT LYR Q
Sbjct: 198 KASFFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVFMGLATMSFFFGTPLYRFQ 257

Query: 241 KPGGSPFTRISQVLVSSFRKYKVKVPESKSLYETADSESSIVGSRKLDHTDDFRFFDKAA 300
           KP GSP T + QVLV+++RK  +KVPE                    D TD         
Sbjct: 258 KPRGSPITSVCQVLVAAYRKSNLKVPE--------------------DSTD--------- 317

Query: 301 VELESDQMLKGSVDPWKLCTVTQVEELKAIIRLLPVWATGIVFAAVYSQMGNLFVLQGDR 360
              E D     + +PWKLCTVTQVEE+K ++RL+P+WA+GI+F+ ++SQ+  LFV QG  
Sbjct: 318 ---EGD----ANTNPWKLCTVTQVEEVKILLRLVPIWASGIIFSVLHSQIYTLFVQQGRC 377

Query: 361 MNIHLGPNFEIPAASLSIFDTLSVIFWVPVYDRIIVPVARKYTGHPNGITQLQRMGIGLL 420
           M   +G  FEIP A+L +FDT SV+  VP+YDR+IVP+ R++TG   G T+LQRMGIGL 
Sbjct: 378 MKRTIG-LFEIPPATLGMFDTASVLISVPIYDRVIVPLVRRFTGLAKGFTELQRMGIGLF 437

Query: 421 ISILAMLSAAILELVRLREVKRHNYYELKH-MPMSIFWQVPQYFLIGCAEVFTFIGQLEF 480
           +S+L++  AAI+E VRL+  +  +  E    +P++IFWQ+PQYFL+G A VF F+G++EF
Sbjct: 438 VSVLSLTFAAIVETVRLQLARDLDLVESGDIVPLNIFWQIPQYFLMGTAGVFFFVGRIEF 497

Query: 481 FYEQAPDAMRSLGSALSLTTVALGNYLSSLLVTIVTNVSTKNGKLGWIP-DNLNYGHIHY 540
           FYEQ+PD+MRSL SA +L T  LGNYLSSL++T+V  +S   GK  WIP DN+N GH+ Y
Sbjct: 498 FYEQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYLS---GKDCWIPSDNINNGHLDY 544

Query: 541 FFFLLALLSVKNLIAFIFIAKWYKYKR 565
           FF+LL  L   N+  F+F +  Y + +
Sbjct: 558 FFWLLVSLGSVNIPVFVFFSVKYTHMK 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148338.10.0e+00100.00protein NRT1/ PTR FAMILY 8.1-like [Momordica charantia][more]
XP_038901014.14.8e-30791.05protein NRT1/ PTR FAMILY 8.1-like [Benincasa hispida][more]
XP_008464060.15.3e-30690.88PREDICTED: protein NRT1/ PTR FAMILY 8.1-like [Cucumis melo] >KAA0061853.1 protei... [more]
KAG6570671.19.0e-30690.70Protein NRT1/ PTR FAMILY 8.1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022986822.11.2e-30590.88protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9M3904.0e-23269.72Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB81.9e-22967.96Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460323.0e-19560.18Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z201.7e-17755.71Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Q84WG02.8e-16152.20Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D3U50.0e+00100.00protein NRT1/ PTR FAMILY 8.1-like OS=Momordica charantia OX=3673 GN=LOC111017019... [more]
A0A5A7V1182.6e-30690.88Protein NRT1/ PTR FAMILY 8.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CL312.6e-30690.88protein NRT1/ PTR FAMILY 8.1-like OS=Cucumis melo OX=3656 GN=LOC103502040 PE=3 S... [more]
A0A6J1J8M55.7e-30690.88protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111484451 PE... [more]
A0A6J1FV987.4e-30690.70protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111448422 ... [more]
Match NameE-valueIdentityDescription
AT3G54140.12.9e-23369.72peptide transporter 1 [more]
AT5G01180.11.3e-23067.96peptide transporter 5 [more]
AT2G02040.12.1e-19660.18peptide transporter 2 [more]
AT1G62200.11.2e-17855.71Major facilitator superfamily protein [more]
AT2G02020.12.0e-16252.20Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 97..526
e-value: 4.3E-127
score: 424.2
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 13..567
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..566
e-value: 4.2E-216
score: 720.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 17..563
NoneNo IPR availablePANTHERPTHR11654:SF297PROTEIN NRT1/ PTR FAMILY 8.3coord: 13..567
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 86..110
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01023PTR2_2coord: 184..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g08260.1Moc02g08260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity