Moc02g06970 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g06970
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	glucan endo-1,3-beta-glucosidase 3-like
Location	chr2: 4931821 .. 4934072 (-)
RNA-Seq Expression	Moc02g06970
Synteny	Moc02g06970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCTCACAGCCCCTCCTCCCACTTCCCACCATATTCATCCCTCCTTCTTCTGTCTCTACCTCAGAGACACCCATTGAAATCCGAGGAGCAAAGATTTTGGAGCAACAACAGATCAAGACAGGGCCTGCCGTGCGAATGGATAATGCAAGTTTCACTTGCACCACTCTGATTCTACTCCTCTTCACTCACTGCATCTCCCCAGGTGATCAATCCACATCAACAAAAACATTTCTTTTCTATCAAATCCATTCAAACCCAGATGTTTGTTTGTTGTTCTTCTCCTTTTCTGTGGGATATATGCTTGTTTTAATTTGGGCTTTCAATAAAGTAGTTCTTGTTCCTCTGTTTTATTCTTCCTTCCCCATACCATCTTTGGAATTTTGAATGCTTTTCCATGTGGGAATCTTTCTCTTTTGACAAATTGTTCAATGATGCGTTGCGTACCCCCAAAGAAAGAAGGAAAGAAAGATTTGTCTCATTACACCAAAAAAAAAACTCGAAAAATCTTGTTTGTTTTGTTGTCAGGGTCAAGTACTTCTTCAATTAGGCAATCCCAAAAAGCAGGGCAAAAGGACATCACCACCCCAATCACGACGGTGCCTACAATTAGTATCCCCACCATGCCCATCGTAAACCCTACTTCCACCCCCGACACCGTGTCTCCGGCCATGGCGACCCCAAGCTTCGGCCCGGCAACCACCGCCGGCGGCTCCAGCTGGTGCATTGCCAGCCTCGGCGCCTCCCAGGCGGCGCTGCAGCTGGCTCTGGACTATGCCTGCGGCGTGGGCGGCGCCGATTGCGGGCCGATTCAGGGCGGCGGAAGGTGCTATAACCCCAATTCGGTTCGCGACCATGCTTCTTACGCCTTCAATAGCTATTACCAGAAGAATCCGCTTCCGAATAGCTGTAATTTTGGAGGGACTGCTGTGATCACCAGCACCAATCCCAGTAAGTTTTCTCCATTTCTTTTTTTTTTTTTCCCCTTCTTCCTCTCGTTTTCTCTATTTCTGTCTGTAATTGCTAATTGGGTTTAGTTTTTAGGGTTTTGAATTTTGAGAAGAAATTGAGCGAATGAATCATTTGCCATTACTATTAGACAACACAATAGCCGTACGCCTAATTTGTAGGGGGTAGAGGCTACCCTGTTACGGACAAAGGACTTTTTAGTTTAGGATTAAGATTTCGCTTAATCATTGCTTTAATTAAAGTGGGTTATAATTATTAATTAGGTTTTTTTAGTTGCGGTGGATCGAACTCACAAGTTGAGTTCGTGGTTGGAGTGGAGGTACATGTCAATCACCGTTGAGCTATGCTCGCTTTGACAATTATTAATTAGGTTAAAATACTAAGTTTGACATGTGTGCTTTTATTTTTGTACTTCTATTTTAAAAATGTTTATTTTGGTCCATTTATTTTAAATTTGGTTCATATTAATTTTTGTATTTGAAAATGCTTATTGTTGTTACGTTACTTTTTAAAAGTGATGATTTTAGTCTATTTTTGCAAAATTTTAACACAAACTTTACACATAACGGAAACTCTTTAATATAAAGTAGTGTTTACATTTTAAGAAACATATTGAATTTGGTGCCGTGTTGAAATTGTGATAGGAAAAAATTGAGATTAAGAAAAAAAATTAATAGACCAAAGTGATTACCTTTTTAAATACATTGAGTAAAATGAACCAAAACGAGAAAATTAGTATTTTAACCCATAAATTACTAGTTTATTCTCTCCATTGATGTTGATTTCAATTTTATTTAACCAACTTTCAATGTAATGTATATAATATGTGTTTAAACTTTAAAATAGGTTGAATAATTTAGACACAGGAGAAAGTTTAGCGTAAGTTTAGCGTAGATGATACATTGTTTTGGACAAAATTTGCCCTATAATAAAATTATTCCGGTTTTCATTCATGTGATGTAAAATTTTTACAGGCAGCGGCACGTGCGAGTACCCATCAACAAGGTGAGCCGCTGCTACCAATATTAAATTTTTTCTCACAAATTTAAATTTGCTTCAATTTTTTAATATAATAAAAAGAAAAATTGGATTATTGCAGCACAAGTTCAACGGTCCTGAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTGTCCCTGCTGGCCGTGGGCCCTCCTCCTCCCACGCAATCAGATCTCGCCACGTGGCGTATTGGGAGTCCTCCCTCTTACTCTTTACAACTCTCCTTTTTGTGGCTTCCCAGTCTAGTTTTAGTTGA

mRNA sequence

ATGTTCTCACAGCCCCTCCTCCCACTTCCCACCATATTCATCCCTCCTTCTTCTGTCTCTACCTCAGAGACACCCATTGAAATCCGAGGAGCAAAGATTTTGGAGCAACAACAGATCAAGACAGGGCCTGCCGTGCGAATGGATAATGCAAGTTTCACTTGCACCACTCTGATTCTACTCCTCTTCACTCACTGCATCTCCCCAGGGTCAAGTACTTCTTCAATTAGGCAATCCCAAAAAGCAGGGCAAAAGGACATCACCACCCCAATCACGACGGTGCCTACAATTAGTATCCCCACCATGCCCATCGTAAACCCTACTTCCACCCCCGACACCGTGTCTCCGGCCATGGCGACCCCAAGCTTCGGCCCGGCAACCACCGCCGGCGGCTCCAGCTGGTGCATTGCCAGCCTCGGCGCCTCCCAGGCGGCGCTGCAGCTGGCTCTGGACTATGCCTGCGGCGTGGGCGGCGCCGATTGCGGGCCGATTCAGGGCGGCGGAAGGTGCTATAACCCCAATTCGGTTCGCGACCATGCTTCTTACGCCTTCAATAGCTATTACCAGAAGAATCCGCTTCCGAATAGCTGTAATTTTGGAGGGACTGCTGTGATCACCAGCACCAATCCCAGCAGCGGCACGTGCGAGTACCCATCAACAAGCACAAGTTCAACGGTCCTGAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTGTCCCTGCTGGCCGTGGGCCCTCCTCCTCCCACGCAATCAGATCTCGCCACGTGGCGTATTGGGAGTCCTCCCTCTTACTCTTTACAACTCTCCTTTTTGTGGCTTCCCAGTCTAGTTTTAGTTGA

Coding sequence (CDS)

ATGTTCTCACAGCCCCTCCTCCCACTTCCCACCATATTCATCCCTCCTTCTTCTGTCTCTACCTCAGAGACACCCATTGAAATCCGAGGAGCAAAGATTTTGGAGCAACAACAGATCAAGACAGGGCCTGCCGTGCGAATGGATAATGCAAGTTTCACTTGCACCACTCTGATTCTACTCCTCTTCACTCACTGCATCTCCCCAGGGTCAAGTACTTCTTCAATTAGGCAATCCCAAAAAGCAGGGCAAAAGGACATCACCACCCCAATCACGACGGTGCCTACAATTAGTATCCCCACCATGCCCATCGTAAACCCTACTTCCACCCCCGACACCGTGTCTCCGGCCATGGCGACCCCAAGCTTCGGCCCGGCAACCACCGCCGGCGGCTCCAGCTGGTGCATTGCCAGCCTCGGCGCCTCCCAGGCGGCGCTGCAGCTGGCTCTGGACTATGCCTGCGGCGTGGGCGGCGCCGATTGCGGGCCGATTCAGGGCGGCGGAAGGTGCTATAACCCCAATTCGGTTCGCGACCATGCTTCTTACGCCTTCAATAGCTATTACCAGAAGAATCCGCTTCCGAATAGCTGTAATTTTGGAGGGACTGCTGTGATCACCAGCACCAATCCCAGCAGCGGCACGTGCGAGTACCCATCAACAAGCACAAGTTCAACGGTCCTGAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTGTCCCTGCTGGCCGTGGGCCCTCCTCCTCCCACGCAATCAGATCTCGCCACGTGGCGTATTGGGAGTCCTCCCTCTTACTCTTTACAACTCTCCTTTTTGTGGCTTCCCAGTCTAGTTTTAGTTGA

Protein sequence

MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILLLFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATPSFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAVPAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS

Homology

[Close]

BLAST of Moc02g06970 vs. NCBI nr
Match: XP_022149616.1 (glucan endo-1,3-beta-glucosidase 3-like [Momordica charantia])

HSP 1 Score: 534.3 bits (1375), Expect = 6.5e-148
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILL 60
           MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILL
Sbjct: 1   MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILL 60

Query: 61  LFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATP 120
           LFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATP
Sbjct: 61  LFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATP 120

Query: 121 SFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHAS 180
           SFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHAS
Sbjct: 121 SFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHAS 180

Query: 181 YAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAV 240
           YAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAV
Sbjct: 181 YAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAV 240

Query: 241 PAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS 281
           PAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS
Sbjct: 241 PAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS 280

[Close]

BLAST of Moc02g06970 vs. NCBI nr
Match: XP_038900832.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 313.5 bits (802), Expect = 1.8e-81
Identity = 171/232 (73.71%), Postives = 188/232 (81.03%), Query Frame = 0


Query: 52  FTCTTLILLLFTHCISPGSSTSSIRQSQ-------KAGQKDITTPITTVPTISIPTMPIV 111
           F C+TLIL LF HC SPGSS +   Q Q        + QKDITTPITTVPT++IPTMPI+
Sbjct: 5   FICSTLILFLFIHCFSPGSSRTDPTQKQGQQFRKSSSNQKDITTPITTVPTVNIPTMPII 64

Query: 112 NPTST-PDTVSPAMATPSFGPATT--AGGSSWCIASLGASQAALQLALDYACGVGGADCG 171
           NPTS+ PDTVSPAM TPSF PATT  +GGSSWCIAS+ ASQAALQLALDYACG+GGADC 
Sbjct: 65  NPTSSNPDTVSPAMTTPSFTPATTTASGGSSWCIASVSASQAALQLALDYACGMGGADCS 124

Query: 172 PIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTST 231
            IQ GG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTA+ITSTNPSSGTCEYPSTST
Sbjct: 125 SIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAMITSTNPSSGTCEYPSTST 184

Query: 232 SSTVLNTTNSSGSTVFGAVPAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFV 274
           SS+VLNTTNSSGSTVFGAVP+G   SS+ A+      Y+ SS L FT L  +
Sbjct: 185 SSSVLNTTNSSGSTVFGAVPSGPSTSSAAALEFPSSQYYSSSFLFFTNLFLL 236

[Close]

BLAST of Moc02g06970 vs. NCBI nr
Match: XP_008456350.1 (PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo] >XP_016901925.1 PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo])

HSP 1 Score: 295.0 bits (754), Expect = 6.6e-76
Identity = 168/236 (71.19%), Postives = 185/236 (78.39%), Query Frame = 0


Query: 52  FTCTTLILLLFTHCISPGSSTSSIRQSQ-----------KAGQKDITTPITTVPTISIPT 111
           F  +TLIL LF HC SPGSS +   Q Q              QKDITTPITTVPTI+IPT
Sbjct: 5   FIYSTLILFLFIHCFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVPTINIPT 64

Query: 112 MPIVNPTST-PDTVSPAMATPSFGPATT-AGGSSWCIASLGASQAALQLALDYACGVGGA 171
           +PI+NPTS+ PDTVSPAM TPSF PATT  GGSSWCIAS  ASQAALQLALDYACG+GGA
Sbjct: 65  IPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYACGMGGA 124

Query: 172 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 231
           DC  IQ GG CYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS
Sbjct: 125 DCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 184

Query: 232 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVA 275
           TSTSS++LNTTNSSGSTVFGAVP+  GPS+S +  + +    +   LL   LLF+A
Sbjct: 185 TSTSSSILNTTNSSGSTVFGAVPS--GPSTSSSSSALNFPSTQFFSLLTNFLLFIA 238

[Close]

BLAST of Moc02g06970 vs. NCBI nr
Match: XP_011656991.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 isoform X1 [Cucumis sativus] >KAE8646882.1 hypothetical protein Csa_021032 [Cucumis sativus])

HSP 1 Score: 293.5 bits (750), Expect = 1.9e-75
Identity = 170/238 (71.43%), Postives = 187/238 (78.57%), Query Frame = 0


Query: 52  FTCTTLILLLFTHCISPGSSTSSIRQSQ-----------KAGQKDITTPITTVPTISIPT 111
           F C+TLIL LF H   PGSS +   Q Q              QKDITTPITTVPTI+IPT
Sbjct: 5   FLCSTLILFLFIHWFCPGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVPTINIPT 64

Query: 112 MPIVNP-TSTPDTVSPAMATPSFGPATT-AGGSSWCIASLGASQAALQLALDYACGVGGA 171
           +PI+NP +S PDTVSPAM TPSF P+TT +GGSSWCIAS  ASQAALQLALDYACG+GGA
Sbjct: 65  IPIINPASSNPDTVSPAMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYACGIGGA 124

Query: 172 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 231
           DC  IQGGG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS+GTCEYPS
Sbjct: 125 DCSSIQGGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSTGTCEYPS 184

Query: 232 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSSHAIRSRHVAYWESSLL-LFTT-LLFVA 275
           TSTSS+VLNTTNSSGSTVFGAVP+  GPSSS +  S  + +  +    LFT  LLF+A
Sbjct: 185 TSTSSSVLNTTNSSGSTVFGAVPS--GPSSSSSSSSSALIFPSTQYFSLFTNFLLFIA 240

[Close]

BLAST of Moc02g06970 vs. NCBI nr
Match: XP_038900831.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 290.4 bits (742), Expect = 1.6e-74
Identity = 161/226 (71.24%), Postives = 180/226 (79.65%), Query Frame = 0


Query: 58  ILLLFTHCISPGSSTSSIRQSQ-------KAGQKDITTPITTVPTISIPTMPIVNPTST- 117
           + L F+  +  GSS +   Q Q        + QKDITTPITTVPT++IPTMPI+NPTS+ 
Sbjct: 36  VYLSFSPNLKSGSSRTDPTQKQGQQFRKSSSNQKDITTPITTVPTVNIPTMPIINPTSSN 95

Query: 118 PDTVSPAMATPSFGPATT--AGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGG 177
           PDTVSPAM TPSF PATT  +GGSSWCIAS+ ASQAALQLALDYACG+GGADC  IQ GG
Sbjct: 96  PDTVSPAMTTPSFTPATTTASGGSSWCIASVSASQAALQLALDYACGMGGADCSSIQAGG 155

Query: 178 RCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLN 237
            CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTA+ITSTNPSSGTCEYPSTSTSS+VLN
Sbjct: 156 NCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAMITSTNPSSGTCEYPSTSTSSSVLN 215

Query: 238 TTNSSGSTVFGAVPAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFV 274
           TTNSSGSTVFGAVP+G   SS+ A+      Y+ SS L FT L  +
Sbjct: 216 TTNSSGSTVFGAVPSGPSTSSAAALEFPSSQYYSSSFLFFTNLFLL 261

[Close]

BLAST of Moc02g06970 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 4.1e-20
Identity = 57/126 (45.24%), Postives = 72/126 (57.14%), Query Frame = 0


Query: 95  TISIPTMPIVNPT-STPDTVSPAMATPSFGPATTAGGSSWCIASLGASQAALQLALDYAC 154
           T  +P  P+  PT S P + SP    P      T G   WC+    A+ A LQ  +DY C
Sbjct: 4   TAGVPDQPVPTPTPSVPTSSSPVPKPP------TQGNKKWCVPKAEATDAQLQSNIDYVC 63

Query: 155 GVGGADCGPIQGGGRCYNPNSVRDHASYAFNSYYQ-KNPLPNSCNFGGTAVITSTNPSSG 214
              G DCGPIQ  G C+NPN+VR HASYA NS+YQ K      C+F GT  ITS++PS+G
Sbjct: 64  SQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPSNG 123

Query: 215 TCEYPS 219
           +C + S
Sbjct: 124 SCSFLS 123

[Close]

BLAST of Moc02g06970 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 2.9e-18
Identity = 55/119 (46.22%), Postives = 72/119 (60.50%), Query Frame = 0


Query: 133 WCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYAFNSYYQKN-P 192
           WC+A   A  ++LQ A+++ACG GGADCGPIQ GG C +P  V+  AS+ FN+YY KN  
Sbjct: 41  WCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFVFNNYYLKNGE 100

Query: 193 LPNSCNFGGTAVITSTNPSSGTCEYPST-STSSTVLNTTNSSGSTVFGAVPAGRGPSSS 250
              +CNF   A +TS NPS GTC+YPS+   ++  L    S G+        GR  SSS
Sbjct: 101 EDEACNFNNNAALTSLNPSQGTCKYPSSKGANNGRLADDTSMGAGQADMSRGGRPISSS 159

[Close]

BLAST of Moc02g06970 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 6.5e-18
Identity = 54/123 (43.90%), Postives = 73/123 (59.35%), Query Frame = 0


Query: 131 SSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYAFNSYYQ-K 190
           +SWC+   G S + LQ  LDYACG  GADC P    G C+NP++VR H +YA NS++Q K
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACG-NGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKK 78

Query: 191 NPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAVPAGRGPSSS 250
                SCNF GTA +T+T+PS   C +PS+++ S      + SGST     P    P  S
Sbjct: 79  GQASESCNFTGTATLTTTDPSYTGCAFPSSASGS------SGSGSTT--VTPGKNSPKGS 132

Query: 251 HAI 253
           ++I
Sbjct: 139 NSI 132

[Close]

BLAST of Moc02g06970 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 8.5e-18
Identity = 52/105 (49.52%), Postives = 66/105 (62.86%), Query Frame = 0


Query: 114 SPAMATPSFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPN 173
           S +  TPS G  T++G   WC+   GA+   LQ +LD+ACG  G DCG IQ GG C+ PN
Sbjct: 349 SSSSQTPS-GKVTSSG---WCVPKKGATNEELQASLDWACG-HGIDCGAIQPGGACFEPN 408

Query: 174 SVRDHASYAFNSYYQKNP-LPNSCNFGGTAVITSTNPSSGTCEYP 218
           +V  HA+YA N Y+QK+P  P  C+F  TA +TS NPS   C YP
Sbjct: 409 NVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

[Close]

BLAST of Moc02g06970 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.2e-17
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 128 AGGSS--WCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYAFNS 187
           AG SS  WC+   G S+A LQ  LDYACG  GADCGPI   G C+NPN+V+ H SYA NS
Sbjct: 14  AGHSSGTWCVCKEGLSEAMLQKTLDYACG-AGADCGPIHQTGPCFNPNTVKSHCSYAVNS 73

Query: 188 YYQKNPLP-NSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGST 236
           ++QK      +C+F GTA  ++++PS  TC +P++++ S       ++ ST
Sbjct: 74  FFQKKGQSLGTCDFAGTATFSASDPSYTTCPFPASASGSGTTTPVTTTPST 123

[Close]

BLAST of Moc02g06970 vs. ExPASy TrEMBL
Match: A0A6J1D8X8 (glucan endo-1,3-beta-glucosidase 3-like OS=Momordica charantia OX=3673 GN=LOC111018000 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.1e-148
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILL 60
           MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILL
Sbjct: 1   MFSQPLLPLPTIFIPPSSVSTSETPIEIRGAKILEQQQIKTGPAVRMDNASFTCTTLILL 60

Query: 61  LFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATP 120
           LFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATP
Sbjct: 61  LFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATP 120

Query: 121 SFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHAS 180
           SFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHAS
Sbjct: 121 SFGPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHAS 180

Query: 181 YAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAV 240
           YAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAV
Sbjct: 181 YAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSSTVLNTTNSSGSTVFGAV 240

Query: 241 PAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS 281
           PAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS
Sbjct: 241 PAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVASQSSFS 280

[Close]

BLAST of Moc02g06970 vs. ExPASy TrEMBL
Match: A0A1S4E1R5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC103496317 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 3.2e-76
Identity = 168/236 (71.19%), Postives = 185/236 (78.39%), Query Frame = 0


Query: 52  FTCTTLILLLFTHCISPGSSTSSIRQSQ-----------KAGQKDITTPITTVPTISIPT 111
           F  +TLIL LF HC SPGSS +   Q Q              QKDITTPITTVPTI+IPT
Sbjct: 5   FIYSTLILFLFIHCFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVPTINIPT 64

Query: 112 MPIVNPTST-PDTVSPAMATPSFGPATT-AGGSSWCIASLGASQAALQLALDYACGVGGA 171
           +PI+NPTS+ PDTVSPAM TPSF PATT  GGSSWCIAS  ASQAALQLALDYACG+GGA
Sbjct: 65  IPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYACGMGGA 124

Query: 172 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 231
           DC  IQ GG CYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS
Sbjct: 125 DCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 184

Query: 232 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSSHAIRSRHVAYWESSLLLFTTLLFVA 275
           TSTSS++LNTTNSSGSTVFGAVP+  GPS+S +  + +    +   LL   LLF+A
Sbjct: 185 TSTSSSILNTTNSSGSTVFGAVPS--GPSTSSSSSALNFPSTQFFSLLTNFLLFIA 238

[Close]

BLAST of Moc02g06970 vs. ExPASy TrEMBL
Match: A0A6J1JFA6 (major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 4.3e-73
Identity = 160/211 (75.83%), Postives = 170/211 (80.57%), Query Frame = 0


Query: 49  NASFTCTTLI--LLLFTHCISPGSSTSSIRQSQ-------KAGQKDITTPITTVPTISIP 108
           +  FTCTTLI  L LF HC  PGSS +   Q Q          QKDITTPITTVPTI+IP
Sbjct: 2   DGGFTCTTLILFLFLFIHCFFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINIP 61

Query: 109 TMPIVNPTSTPDTVSPAMATPSFGPA-TTAGGSSWCIASLGASQAALQLALDYACGVGGA 168
           T      TS PD+VSP + TPSF PA TT GGSSWCIA+LGASQAALQLALDYACG+GGA
Sbjct: 62  T------TSNPDSVSPTITTPSFTPATTTTGGSSWCIANLGASQAALQLALDYACGMGGA 121

Query: 169 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 228
           DC  IQ GGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG+CEYPS
Sbjct: 122 DCSAIQTGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPS 181

Query: 229 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSS 250
           TSTSS+VLNTTNSSGSTVFGAVP+G   SSS
Sbjct: 182 TSTSSSVLNTTNSSGSTVFGAVPSGPSTSSS 206

[Close]

BLAST of Moc02g06970 vs. ExPASy TrEMBL
Match: A0A6J1FWZ7 (major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC111448235 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 2.1e-72
Identity = 158/211 (74.88%), Postives = 169/211 (80.09%), Query Frame = 0


Query: 49  NASFTCTTLI--LLLFTHCISPGSSTSSIRQSQ-------KAGQKDITTPITTVPTISIP 108
           +  FTCTTLI  L LF HC+ PGSS +   Q Q          QKDITTPITTVPTI+IP
Sbjct: 2   DGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINIP 61

Query: 109 TMPIVNPTSTPDTVSPAMATPSFGPATTA-GGSSWCIASLGASQAALQLALDYACGVGGA 168
           T      TS PD+VSP + TPSF PATTA GGSSWCIASLGASQA LQLALDYACG+GGA
Sbjct: 62  T------TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGA 121

Query: 169 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 228
           DC  IQ GG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG+CEYPS
Sbjct: 122 DCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPS 181

Query: 229 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSS 250
           TSTSS++LNTTNSSGSTVFGA P+G   SSS
Sbjct: 182 TSTSSSILNTTNSSGSTVFGAAPSGPSTSSS 206

[Close]

BLAST of Moc02g06970 vs. ExPASy TrEMBL
Match: A0A0A0KDA3 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.8e-63
Identity = 136/181 (75.14%), Postives = 146/181 (80.66%), Query Frame = 0


Query: 52  FTCTTLILLLFTHCISPGSSTSSIRQSQ-----------KAGQKDITTPITTVPTISIPT 111
           F C+TLIL LF H   PGSS +   Q Q              QKDITTPITTVPTI+IPT
Sbjct: 5   FLCSTLILFLFIHWFCPGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVPTINIPT 64

Query: 112 MPIVNP-TSTPDTVSPAMATPSFGPATT-AGGSSWCIASLGASQAALQLALDYACGVGGA 171
           +PI+NP +S PDTVSPAM TPSF P+TT +GGSSWCIAS  ASQAALQLALDYACG+GGA
Sbjct: 65  IPIINPASSNPDTVSPAMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYACGIGGA 124

Query: 172 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 220
           DC  IQGGG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS+GTCEYPS
Sbjct: 125 DCSSIQGGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSTGTCEYPS 184

[Close]

BLAST of Moc02g06970 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.4e-39
Identity = 98/202 (48.51%), Postives = 126/202 (62.38%), Query Frame = 0


Query: 47  MDNASFTCTTLILLLFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNP 106
           MD      T+L+LL F       S T +I +  +  +KDITTP+ T PT + PT  + N 
Sbjct: 1   MDKGFRLVTSLLLLSFFF-----SGTKAITEPIEE-EKDITTPLATNPT-TTPTTVVPNS 60

Query: 107 TSTPDTVSPAMATPSFGPATTA--GGSSWCIASLGASQAALQLALDYACGVGGADCGPIQ 166
            S    V+    T    P   A  G  SWC+A    ++ ALQ ALDYACG+GGADC  IQ
Sbjct: 61  DSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENVAKMALQAALDYACGIGGADCSEIQ 120

Query: 167 GGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTSTSST 226
            GG CYNPNS+R HAS+AFNSYYQKNP+P+SCNF GTA+  S +PS G+C +PSTSTS +
Sbjct: 121 EGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDGTAITISADPSLGSCHFPSTSTSES 180

Query: 227 VLNTTNSSGSTVFGAVPAGRGP 247
           +LN T+  G  +FG +P+   P
Sbjct: 181 ILNVTSEDGLGLFGRIPSHPTP 195

[Close]

BLAST of Moc02g06970 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 132.5 bits (332), Expect = 5.3e-31
Identity = 82/166 (49.40%), Postives = 102/166 (61.45%), Query Frame = 0


Query: 47  MDNASFTCTTLILLLFTHCISPGSSTSSIRQSQKAGQKDITTPITTVPTISIPTMPIVNP 106
           MD      T+L+LL F       S T +I +  +  +KDITTP+ T PT + PT  + N 
Sbjct: 1   MDKGFRLVTSLLLLSFFF-----SGTKAITEPIEE-EKDITTPLATNPT-TTPTTVVPNS 60

Query: 107 TSTPDTVSPAMATPSFGPATTA--GGSSWCIASLGASQAALQLALDYACGVGGADCGPIQ 166
            S    V+    T    P   A  G  SWC+A    ++ ALQ ALDYACG+GGADC  IQ
Sbjct: 61  DSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENVAKMALQAALDYACGIGGADCSEIQ 120

Query: 167 GGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 211
            GG CYNPNS+R HAS+AFNSYYQKNP+P+SCNF GTA+  S +PS
Sbjct: 121 EGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDGTAITISADPS 159

[Close]

BLAST of Moc02g06970 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 2.9e-29
Identity = 60/98 (61.22%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 123 GPATTAGGSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYA 182
           G     G   WCIA   AS  +LQ+ALDYACG GGADCG IQ G  CY PN++RDHAS+A
Sbjct: 138 GAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFA 197

Query: 183 FNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPSTS 221
           FNSYYQK+P  +SCNFGG A +TST+PS G+C + S+S
Sbjct: 198 FNSYYQKHPGSDSCNFGGAAQLTSTDPSKGSCHFSSSS 235

[Close]

BLAST of Moc02g06970 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 125.9 bits (315), Expect = 4.9e-29
Identity = 72/152 (47.37%), Postives = 97/152 (63.82%), Query Frame = 0


Query: 86  ITTPITTVPTISIPTMPIVNPTSTPDTVSPAMATPSFGPATTAGGSSWCIASLGASQAAL 145
           +T P+T   T  +   P   P+ T     P +A P    + +  G SWC+A  GASQ +L
Sbjct: 90  LTPPVTNPVTNPVTQYPPTQPSGTVPVPVPVVAPPVVSNSPSVSGQSWCVAKPGASQVSL 149

Query: 146 QLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVIT 205
           Q ALDYACG+  ADC  +Q GG CY+P S++ HAS+AFNSYYQKNP P SC+FGG A + 
Sbjct: 150 QQALDYACGI--ADCSQLQQGGNCYSPISLQSHASFAFNSYYQKNPSPQSCDFGGAASLV 209

Query: 206 STNPSSGTCEYPSTSTSSTVL--NTTNSSGST 236
           +TNPS+G+C Y + S++ST +   TT  + ST
Sbjct: 210 NTNPSTGSCIYQTGSSTSTPMTAGTTTPTPST 239

[Close]

BLAST of Moc02g06970 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 105.1 bits (261), Expect = 9.0e-23
Identity = 51/90 (56.67%), Postives = 61/90 (67.78%), Query Frame = 0


Query: 130 GSSWCIASLGASQAALQLALDYACGVGGADCGPIQGGGRCYNPNSVRDHASYAFNSYYQK 189
           G +WC+A+   ++  LQ  LDYACG GGADC PIQ G  CYNP S+  HASYAFNSYYQK
Sbjct: 369 GQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAHASYAFNSYYQK 428

Query: 190 NPL-PNSCNFGGTAVITSTNPSSGTCEYPS 219
           N     +CNFGG A + S  P  G CE+P+
Sbjct: 429 NARGVGTCNFGGAAYVVSQPPKYGKCEFPT 458

The following BLAST results are available for this feature:

BLAST of Moc02g06970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149616.1	6.5e-148	100.00	glucan endo-1,3-beta-glucosidase 3-like [Momordica charantia]	[more]
XP_038900832.1	1.8e-81	73.71	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X2 [Benincasa hispida]	[more]
XP_008456350.1	6.6e-76	71.19	PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo] >XP_01690...	[more]
XP_011656991.1	1.9e-75	71.43	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 isoform X1 [Cucumis sativus] >KAE8646882...	[more]
XP_038900831.1	1.6e-74	71.24	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 [Benincasa hispida]	[more]

BLAST of Moc02g06970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84V39	4.1e-20	45.24	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9M2K6	2.9e-18	46.22	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]
Q9SD84	6.5e-18	43.90	PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2...	[more]
Q9M069	8.5e-18	49.52	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q9FZ86	3.2e-17	45.95	PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3...	[more]

BLAST of Moc02g06970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8X8	3.1e-148	100.00	glucan endo-1,3-beta-glucosidase 3-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A1S4E1R5	3.2e-76	71.19	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1JFA6	4.3e-73	75.83	major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 ...	[more]
A0A6J1FWZ7	2.1e-72	74.88	major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC11144823...	[more]
A0A0A0KDA3	1.8e-63	75.14	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=...	[more]

BLAST of Moc02g06970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30933.1	1.4e-39	48.51	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	5.3e-31	49.40	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	2.9e-29	61.22	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	4.9e-29	47.37	Carbohydrate-binding X8 domain superfamily protein	[more]
AT5G55180.1	9.0e-23	56.67	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 132..216 e-value: 1.5E-44 score: 164.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 133..202 e-value: 1.1E-19 score: 70.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 127..234 e-value: 8.5E-11 score: 44.1
None	No IPR available	PANTHER	PTHR31044:SF53	CARBOHYDRATE-BINDING X8 DOMAIN PROTEIN	coord: 89..257
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 89..257

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g06970.1	Moc02g06970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds