Moc02g05940 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g05940
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	O-methyltransferase, putative
Location	chr2: 4278017 .. 4279159 (-)
RNA-Seq Expression	Moc02g05940
Synteny	Moc02g05940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCCAAAGAAGCAAAAGAAACAGAGGCAGAGCTTAGAGGGCAAGCAGACATATGGAGATATATGCTCTGTTTTGCAGATTCAATGGCCTTGAAATCTGCCGTTCAACTTCAATTAGCTGACATTATTAACTCTCATGGCTCTCCCATCACTCTCTCTCAGATTGCTTCCTCCATTGCTTCTTCCTCTGCCTCTCCAAACATTTCCTACCTCGCTCGCATCATGAGACTCCTCGTCCGCCGCAACATCTTCGCCGCACACCACCCATCCGACGGCGGCGACACCCTCTACGGCCTCACCCACTCCTCCCGGTGGCTCCTCCGCGACTCCGACCTCACCCTCGCCCCCATGATACTCGCCGAGCTTCACCACTGGCTCGTCGACCCATGGCACTGTTTCGCTGAAGGCGTCAAAGAAGGCGGCGACATCTTCCAGAAGGCCAACGGCCACGATATTTGGGGCTTCGCTTCCAAAAACCCCCAATTCAACCAATTGTTCAACGACGCCATGGCCTCCACTTCCAAGGTCGTCATTAACGCAATCCTCTCTGTTTACAAAGACGGGTTCAACTCGGTGGGGTCGCTGGCGGACGTCGGCGGCGGAATCGGGGGCGCCGTGTCGGAGATCGTGAAAGCGTACCCCCACATGAAGGGCATCAACTACGACTTGCCGCACGTGGTGAGCACGGCGACGGCGTACCCAGGGGTGACCCACGTGGGAGGCGACATGTTTGAGAGCGTTCCCAAGGCCGACGCCATTTTTATGAAGGTGATCCATTAAACTAATCAATGAATTCCATCCATTAATTGGTATAATTATGAGTTGAATTGGTGAATGGTGCAGTGGATACTGCACGACTGGGACGACAAGGAGTGCGTGAGAATTCTGGAGAATTGCAGGAAGGCGATACCGGAGAAAACAGGGAAAGTGATCATAGTGGAAGTGGTGCTGAATCCGGAGGGAGAAGGCGCATTTGACGACACTCGTTTATACTTTGATCTGCTGATGTTGGCGCATACCAATGGAAAAGAGAGGACAGAGAAGGAGTGGAAGAAAATATTGGAGGAAGCAGGATTCCGTCGCTACAAACTCGTTACTCTTCCTGCTCTGACTTCCATTATTGAAGCATATCCATTGTGA

mRNA sequence

ATGGAAGCCAAAGAAGCAAAAGAAACAGAGGCAGAGCTTAGAGGGCAAGCAGACATATGGAGATATATGCTCTGTTTTGCAGATTCAATGGCCTTGAAATCTGCCGTTCAACTTCAATTAGCTGACATTATTAACTCTCATGGCTCTCCCATCACTCTCTCTCAGATTGCTTCCTCCATTGCTTCTTCCTCTGCCTCTCCAAACATTTCCTACCTCGCTCGCATCATGAGACTCCTCGTCCGCCGCAACATCTTCGCCGCACACCACCCATCCGACGGCGGCGACACCCTCTACGGCCTCACCCACTCCTCCCGGTGGCTCCTCCGCGACTCCGACCTCACCCTCGCCCCCATGATACTCGCCGAGCTTCACCACTGGCTCGTCGACCCATGGCACTGTTTCGCTGAAGGCGTCAAAGAAGGCGGCGACATCTTCCAGAAGGCCAACGGCCACGATATTTGGGGCTTCGCTTCCAAAAACCCCCAATTCAACCAATTGTTCAACGACGCCATGGCCTCCACTTCCAAGGTCGTCATTAACGCAATCCTCTCTGTTTACAAAGACGGGTTCAACTCGGTGGGGTCGCTGGCGGACGTCGGCGGCGGAATCGGGGGCGCCGTGTCGGAGATCGTGAAAGCGTACCCCCACATGAAGGGCATCAACTACGACTTGCCGCACGTGGTGAGCACGGCGACGGCGTACCCAGGGGTGACCCACGTGGGAGGCGACATGTTTGAGAGCGTTCCCAAGGCCGACGCCATTTTTATGAAGTGGATACTGCACGACTGGGACGACAAGGAGTGCGTGAGAATTCTGGAGAATTGCAGGAAGGCGATACCGGAGAAAACAGGGAAAGTGATCATAGTGGAAGTGGTGCTGAATCCGGAGGGAGAAGGCGCATTTGACGACACTCGTTTATACTTTGATCTGCTGATGTTGGCGCATACCAATGGAAAAGAGAGGACAGAGAAGGAGTGGAAGAAAATATTGGAGGAAGCAGGATTCCGTCGCTACAAACTCGTTACTCTTCCTGCTCTGACTTCCATTATTGAAGCATATCCATTGTGA

Coding sequence (CDS)

ATGGAAGCCAAAGAAGCAAAAGAAACAGAGGCAGAGCTTAGAGGGCAAGCAGACATATGGAGATATATGCTCTGTTTTGCAGATTCAATGGCCTTGAAATCTGCCGTTCAACTTCAATTAGCTGACATTATTAACTCTCATGGCTCTCCCATCACTCTCTCTCAGATTGCTTCCTCCATTGCTTCTTCCTCTGCCTCTCCAAACATTTCCTACCTCGCTCGCATCATGAGACTCCTCGTCCGCCGCAACATCTTCGCCGCACACCACCCATCCGACGGCGGCGACACCCTCTACGGCCTCACCCACTCCTCCCGGTGGCTCCTCCGCGACTCCGACCTCACCCTCGCCCCCATGATACTCGCCGAGCTTCACCACTGGCTCGTCGACCCATGGCACTGTTTCGCTGAAGGCGTCAAAGAAGGCGGCGACATCTTCCAGAAGGCCAACGGCCACGATATTTGGGGCTTCGCTTCCAAAAACCCCCAATTCAACCAATTGTTCAACGACGCCATGGCCTCCACTTCCAAGGTCGTCATTAACGCAATCCTCTCTGTTTACAAAGACGGGTTCAACTCGGTGGGGTCGCTGGCGGACGTCGGCGGCGGAATCGGGGGCGCCGTGTCGGAGATCGTGAAAGCGTACCCCCACATGAAGGGCATCAACTACGACTTGCCGCACGTGGTGAGCACGGCGACGGCGTACCCAGGGGTGACCCACGTGGGAGGCGACATGTTTGAGAGCGTTCCCAAGGCCGACGCCATTTTTATGAAGTGGATACTGCACGACTGGGACGACAAGGAGTGCGTGAGAATTCTGGAGAATTGCAGGAAGGCGATACCGGAGAAAACAGGGAAAGTGATCATAGTGGAAGTGGTGCTGAATCCGGAGGGAGAAGGCGCATTTGACGACACTCGTTTATACTTTGATCTGCTGATGTTGGCGCATACCAATGGAAAAGAGAGGACAGAGAAGGAGTGGAAGAAAATATTGGAGGAAGCAGGATTCCGTCGCTACAAACTCGTTACTCTTCCTGCTCTGACTTCCATTATTGAAGCATATCCATTGTGA

Protein sequence

MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMILAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGAFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL

Homology

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BLAST of Moc02g05940 vs. NCBI nr
Match: XP_022140296.1 (xanthohumol 4'-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 718.8 bits (1854), Expect = 2.3e-203
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI
Sbjct: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60

Query: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120
           ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180
           AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN
Sbjct: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180

Query: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240

Query: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300

Query: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL 356
           FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL
Sbjct: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL 355

[Close]

BLAST of Moc02g05940 vs. NCBI nr
Match: XP_022140297.1 (xanthohumol 4'-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 648.7 bits (1672), Expect = 3.0e-182
Identity = 318/354 (89.83%), Postives = 335/354 (94.63%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           ME KEA E EA LRGQADIWRYMLCFADSMALKSAVQL+LADII SHGSP+TLSQIA+SI
Sbjct: 1   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 60

Query: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120
           ASSSASPNIS+LARIM LLVRRNIFAAHHPSDGG+TLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180
           AELH WL+DPWH FA+GV+EGG++F KANG   W FA++NPQFN+LFNDAMASTSKVVIN
Sbjct: 121 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 180

Query: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTA AYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 240

Query: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENC+KAIPEKTGKVIIVEVVLNPEGEG 
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 300

Query: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           FDDT LYFDLLML H+NGKERTEKEWKKILEEAGF RYKL+ LPA+TSIIEAYP
Sbjct: 301 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 354

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BLAST of Moc02g05940 vs. NCBI nr
Match: XP_038902222.1 (xanthohumol 4-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 595.9 bits (1535), Expect = 2.3e-166
Identity = 290/357 (81.23%), Postives = 321/357 (89.92%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           MEAKE KETE  L+GQ+ IW+YMLCFADSMALK AV+L L DIINSHGSPI+LSQIASSI
Sbjct: 1   MEAKETKETEDLLKGQSVIWKYMLCFADSMALKCAVELHLPDIINSHGSPISLSQIASSI 60

Query: 61  ASS--SASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPM 120
           A+S  S+SP ISYL RIMRLLVRRNIF AHHPS+GG+TLYGLTHSS+WLLRDS L+LAPM
Sbjct: 61  AASNPSSSPQISYLTRIMRLLVRRNIFTAHHPSNGGETLYGLTHSSKWLLRDSPLSLAPM 120

Query: 121 ILAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVV 180
           + +ELH W+V+PW CF + VKEGG  FQ ANG DIW FASKNPQFN  FNDAMASTSKVV
Sbjct: 121 VFSELHQWMVNPWLCFTQAVKEGGSPFQIANGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 181 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVT 240
           +NAILSVYKDGFNSV SLADVGGGIGG+VSEIVKA+PH+KGINYDLPHV+STA  Y GVT
Sbjct: 181 MNAILSVYKDGFNSVQSLADVGGGIGGSVSEIVKAFPHVKGINYDLPHVISTAPLYHGVT 240

Query: 241 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGE 300
           H+GGDMF+ +PKADAIFMKWILHDW+DKECV+ILENC+KAIPEKTGKVIIVEVVLN +GE
Sbjct: 241 HIGGDMFDYIPKADAIFMKWILHDWNDKECVKILENCKKAIPEKTGKVIIVEVVLNEDGE 300

Query: 301 GAFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL 356
           G FDDTR YFDLLMLAHTNGKERTEKEWK ILEEAGF RY+++ LPALTSIIEAYPL
Sbjct: 301 GTFDDTRFYFDLLMLAHTNGKERTEKEWKTILEEAGFPRYRVIPLPALTSIIEAYPL 357

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BLAST of Moc02g05940 vs. NCBI nr
Match: XP_008448276.2 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo] >KAA0062101.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK04926.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 593.2 bits (1528), Expect = 1.5e-165
Identity = 288/356 (80.90%), Postives = 317/356 (89.04%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           MEAKE  E EA L+GQA+IW+YMLCFADSMALK AV+L LADIINSHGSPI+LSQI+SSI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 61  ASS--SASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPM 120
           A+S  S+SP ISYL RIMRLLVRRNIFAAHHPSDGGDTLYGLTHSS+WLLRDS LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 121 ILAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVV 180
             +ELH W+V PW CF E VKEGG  F+ A+G DIW FASKNPQFN  FNDAMASTSKVV
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 181 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVT 240
           +NAILSVY+DGFNS+ SLADVGGGIGG++SEIVKA+PH+KGINYDLPHV+STA  Y GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 241 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGE 300
           H+GGDMFE +PK DAIFMKWILHDW+DKECV+ILENC+KAIPEK GKVIIVEVVLN EG+
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 301 GAFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           GAFDDT+ YFDLLMLAHTNGKERTEKEWK ILEEAGF RY L+ LPAL SIIEAYP
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYP 356

[Close]

BLAST of Moc02g05940 vs. NCBI nr
Match: XP_022946951.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 589.7 bits (1519), Expect = 1.6e-164
Identity = 278/355 (78.31%), Postives = 322/355 (90.70%), Query Frame = 0


Query: 2   EAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIA 61
           E +E +ETE  L+GQA+IW+YMLCFADSMALK AV+L LADIINSHGSPI+LSQIASSI 
Sbjct: 5   ETEETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIV 64

Query: 62  SSS--ASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMI 121
           +S   A+P ISYL+RIMRLLVRRNIFA HH SDGG+ LYGLTHSS+WLLRD++LTLAPM+
Sbjct: 65  ASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMV 124

Query: 122 LAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVI 181
           LAELH W+VDPW CF++GVKEGGD FQKANG DIW FAS NPQFNQLFN+AMASTSKV++
Sbjct: 125 LAELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIM 184

Query: 182 NAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTH 241
            AILSVY+DGF+S+ SL DVGGGIGG +SEIVKA+PH+KGIN+DLPHV+STA  Y GVTH
Sbjct: 185 KAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINFDLPHVISTAPPYQGVTH 244

Query: 242 VGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEG 301
           +GGDMFE++PKADA+FMKWILHDWDDKECVRIL+NC+KAIPEKTGKVI+VEV+L  +G+G
Sbjct: 245 IGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDG 304

Query: 302 AFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           AFDDTR YFDLLMLAHTNG+ERTEKEWK ILEEAGF RY+++ LPA+TSIIEAYP
Sbjct: 305 AFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYP 359

[Close]

BLAST of Moc02g05940 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.2e-112
Identity = 201/360 (55.83%), Postives = 267/360 (74.17%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           ME     +TEA LRG+A++W+ +   AD M +K A++L++ DI++SH +PITL+QIASS+
Sbjct: 1   MELARNDQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSV 60

Query: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDL----TLA 120
              S S N+SYL+RIMRLLVRR IF+ H   DG + LYG THSSR LL  + L    TLA
Sbjct: 61  -PDSPSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLA 120

Query: 121 PMILAELHHWLVDPWHCFAEGVKEGGDIFQKAN-GHDIWGFASKNPQFNQLFNDAMASTS 180
           P +    H +L  PW C A  VKEGG+ F+  + G  +W  +  NP+FN++FND MAST+
Sbjct: 121 PFVAFMTHPYLSAPWSCLARCVKEGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTA 180

Query: 181 KVVINAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYP 240
           ++   AILS Y+D F  + SL DVGG  GG++S IVK++PH+KGINYDLPHVV+TA  Y 
Sbjct: 181 RITTMAILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYT 240

Query: 241 G-VTHVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLN 300
           G V+HVGG+MFE +P A A+FMKWILHDW D++CV+IL+NCR+A+PEK GK+IIV++VL 
Sbjct: 241 GLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVLE 300

Query: 301 PEGEGAFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           PEG G FDD  +  D+ ++A T GKERTEKEWK++LEE GF RY+++ +PALTS+IEAYP
Sbjct: 301 PEGNGLFDDAAVMLDIALMALTRGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAYP 359

[Close]

BLAST of Moc02g05940 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 8.9e-105
Identity = 188/347 (54.18%), Postives = 255/347 (73.49%), Query Frame = 0


Query: 13  LRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIAS---SSASPNI 72
           LRGQ  IW+ M  F DSMALK A++L++ADII+SHG PITLSQIAS I S   SS SPNI
Sbjct: 4   LRGQEQIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNI 63

Query: 73  SYLARIMRLLVRRNIFAAH-HPSDGGDTLYGLTHSSRWLLRDSDLTLAPMILAELHHWLV 132
            YL+RIMR LVR+NIF  H   +D   +LYGL+ SSRWLLRD   +LAPM+L + H   +
Sbjct: 64  PYLSRIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSM 123

Query: 133 DPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKD 192
             WH   + V+   + F+KA+G +IW F+S NP FN++FN+AMAS   + + A+LS YKD
Sbjct: 124 AVWHFLEDYVRNSSNTFEKAHGCNIWEFSSANPDFNKIFNNAMASIVPIYMGAVLSSYKD 183

Query: 193 GFNSV-GSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFES 252
           G   + G++ DVGGG GG++SE++K YP++KGIN+DLPHV++TA A  GVTH+ GD+FES
Sbjct: 184 GLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDLPHVIATAPALDGVTHISGDIFES 243

Query: 253 VPKADAIFMKWILHDWDDKECVRILENCRKAIPE-KTGKVIIVEVVLNPEGEGAFDDTRL 312
           +P ADA+ MK +LH + D++CV++L NCRKAI + K GK+II+E+VL+P     FD+TR+
Sbjct: 244 IPSADAVLMKGVLHCFSDEKCVKVLRNCRKAITDKKNGKIIILEIVLDPTSNQIFDETRM 303

Query: 313 YFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAY 354
            +DLL+   + GKERTE EWK++L EAGF   K+  +P + +IIEA+
Sbjct: 304 VYDLLIPLFSGGKERTELEWKRLLNEAGFTSIKITKIPIIPAIIEAF 350

[Close]

BLAST of Moc02g05940 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.2e-103
Identity = 184/350 (52.57%), Postives = 245/350 (70.00%), Query Frame = 0


Query: 10  EAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIASS--SASP 69
           E  L+GQA+IW +M  F DSMALK AV+L + DIINSHG P+T+S+I  S+ ++  S+SP
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 70  NISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMILAELHHWL 129
           NI YL RIMRLLV + +F +    +    LY LT SS+WLL+DS   L+P++L E +  L
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 130 VDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYK 189
           + PW    +  +E    F++A+G +IW  A  +P+FN   N AM  ++  +IN +L  YK
Sbjct: 125 LKPWQYLGKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLEYK 184

Query: 190 DGFNSV-GSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFE 249
           DGF+ + GSL DVGGG G  ++EIVKA+PH++GIN+DLPHVV+TA  +PGV HVGGDMF 
Sbjct: 185 DGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDMFV 244

Query: 250 SVPKADAIFMKWILHDWDDKECVRILENCRKAI-PEKTGKVIIVEVVLNPEGEGAFDDTR 309
            +P+ADA+ MKWILHDW D++C  IL+NC +AI  +K GKVIIV+ VL P+G   FD   
Sbjct: 245 DIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDKMG 304

Query: 310 LYFDLLMLAHTN-GKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           L FD+LM+AHT  GKERTE EWK +L  AGF RY ++  PA   IIEA+P
Sbjct: 305 LIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

[Close]

BLAST of Moc02g05940 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.8e-81
Identity = 153/343 (44.61%), Postives = 229/343 (66.76%), Query Frame = 0


Query: 13  LRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIASSSASPNISYL 72
           L  QA +W ++  FA+S+ LK AVQL LA+II++ G+ +TLS+++S + S   + +  Y 
Sbjct: 8   LSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDALY- 67

Query: 73  ARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMILAELHHWLVDPWH 132
            R+MR LV   +F     S  G+  YGL   +++L++  D  +   ILA      + PWH
Sbjct: 68  -RVMRYLVHMKLFT--KASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWH 127

Query: 133 CFAEGVK-EGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKDGFN 192
              +G+  E G  F+KA G +IWG+ +++P+ NQLFN+AMA+ S+++++A++    + FN
Sbjct: 128 YLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFN 187

Query: 193 SVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFESVPKA 252
            + +L DVGGG G AV  I  A+PH+K   YDLPHV++ +  Y  V  V GDMF+ +PKA
Sbjct: 188 GITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPKA 247

Query: 253 DAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGAFDDTRLYFDLL 312
           DAI MK ILHDWDDKEC+ IL+ C++A+P K GKVIIV++VLN + E  +   RL  DL 
Sbjct: 248 DAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLDLD 307

Query: 313 MLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           M+ +T GKERTE+EWKK++ +AG++ +K+  + A+ S+IEAYP
Sbjct: 308 MMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

[Close]

BLAST of Moc02g05940 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.9e-79
Identity = 145/345 (42.03%), Postives = 224/345 (64.93%), Query Frame = 0


Query: 13  LRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIASSSASPNISYL 72
           L  QA +W ++  FADS+ LKSAVQL LA+II++HGSP+TLS+++  + S   + +  Y 
Sbjct: 9   LSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDALY- 68

Query: 73  ARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMILAELHHWLVDPWH 132
            R++R LV   +F     S  G+  YGL   +++L++  D  +   IL       + PWH
Sbjct: 69  -RVLRYLVHMKLFT--KSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPWH 128

Query: 133 CFAEGVKEGGD---IFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKDG 192
              EG+   G     F+KA G +IW + +++P+ NQLFN+ MA+ ++++++A++      
Sbjct: 129 YLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSSM 188

Query: 193 FNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFESVP 252
           F+ + ++ DVGGG G AV  I KA+PH+K   YDLPHV++ +  Y  +  + GDMF+ +P
Sbjct: 189 FDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYIP 248

Query: 253 KADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGAFDDTRLYFD 312
            ADAI MK ILHDWDDKEC+ IL+ C+ A+P   GKVII++++L+ + E  +   RL  D
Sbjct: 249 NADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTLD 308

Query: 313 LLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           L M+ +T GKERTE+EWKK++ +AG++ YK+  + A+ S+IEAYP
Sbjct: 309 LDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349

[Close]

BLAST of Moc02g05940 vs. ExPASy TrEMBL
Match: A0A6J1CFN9 (xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111010999 PE=3 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.1e-203
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI
Sbjct: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60

Query: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120
           ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180
           AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN
Sbjct: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180

Query: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240

Query: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300

Query: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL 356
           FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL
Sbjct: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYPL 355

[Close]

BLAST of Moc02g05940 vs. ExPASy TrEMBL
Match: A0A6J1CGG0 (xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111011000 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.4e-182
Identity = 318/354 (89.83%), Postives = 335/354 (94.63%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           ME KEA E EA LRGQADIWRYMLCFADSMALKSAVQL+LADII SHGSP+TLSQIA+SI
Sbjct: 1   MEPKEANEAEAMLRGQADIWRYMLCFADSMALKSAVQLRLADIIKSHGSPLTLSQIAASI 60

Query: 61  ASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMIL 120
           ASSSASPNIS+LARIM LLVRRNIFAAHHPSDGG+TLYGLTHSSRWLLRDSDLTLAPMIL
Sbjct: 61  ASSSASPNISFLARIMALLVRRNIFAAHHPSDGGETLYGLTHSSRWLLRDSDLTLAPMIL 120

Query: 121 AELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVIN 180
           AELH WL+DPWH FA+GV+EGG++F KANG   W FA++NPQFN+LFNDAMASTSKVVIN
Sbjct: 121 AELHPWLLDPWHRFAQGVREGGNLFHKANGLHFWDFAARNPQFNRLFNDAMASTSKVVIN 180

Query: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHV 240
           AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTA AYPGVTHV
Sbjct: 181 AILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTAPAYPGVTHV 240

Query: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGA 300
           GGDMFESVPKADAIFMKWILHDWDDKECVRILENC+KAIPEKTGKVIIVEVVLNPEGEG 
Sbjct: 241 GGDMFESVPKADAIFMKWILHDWDDKECVRILENCKKAIPEKTGKVIIVEVVLNPEGEGP 300

Query: 301 FDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           FDDT LYFDLLML H+NGKERTEKEWKKILEEAGF RYKL+ LPA+TSIIEAYP
Sbjct: 301 FDDTGLYFDLLMLVHSNGKERTEKEWKKILEEAGFLRYKLLALPAITSIIEAYP 354

[Close]

BLAST of Moc02g05940 vs. ExPASy TrEMBL
Match: A0A5A7V8T5 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001350 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 7.2e-166
Identity = 288/356 (80.90%), Postives = 317/356 (89.04%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           MEAKE  E EA L+GQA+IW+YMLCFADSMALK AV+L LADIINSHGSPI+LSQI+SSI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 61  ASS--SASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPM 120
           A+S  S+SP ISYL RIMRLLVRRNIFAAHHPSDGGDTLYGLTHSS+WLLRDS LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 121 ILAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVV 180
             +ELH W+V PW CF E VKEGG  F+ A+G DIW FASKNPQFN  FNDAMASTSKVV
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 181 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVT 240
           +NAILSVY+DGFNS+ SLADVGGGIGG++SEIVKA+PH+KGINYDLPHV+STA  Y GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 241 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGE 300
           H+GGDMFE +PK DAIFMKWILHDW+DKECV+ILENC+KAIPEK GKVIIVEVVLN EG+
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 301 GAFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           GAFDDT+ YFDLLMLAHTNGKERTEKEWK ILEEAGF RY L+ LPAL SIIEAYP
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYP 356

[Close]

BLAST of Moc02g05940 vs. ExPASy TrEMBL
Match: A0A1S3BJA7 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490512 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 7.2e-166
Identity = 288/356 (80.90%), Postives = 317/356 (89.04%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSI 60
           MEAKE  E EA L+GQA+IW+YMLCFADSMALK AV+L LADIINSHGSPI+LSQI+SSI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 61  ASS--SASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPM 120
           A+S  S+SP ISYL RIMRLLVRRNIFAAHHPSDGGDTLYGLTHSS+WLLRDS LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 121 ILAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVV 180
             +ELH W+V PW CF E VKEGG  F+ A+G DIW FASKNPQFN  FNDAMASTSKVV
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 181 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVT 240
           +NAILSVY+DGFNS+ SLADVGGGIGG++SEIVKA+PH+KGINYDLPHV+STA  Y GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 241 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGE 300
           H+GGDMFE +PK DAIFMKWILHDW+DKECV+ILENC+KAIPEK GKVIIVEVVLN EG+
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 301 GAFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           GAFDDT+ YFDLLMLAHTNGKERTEKEWK ILEEAGF RY L+ LPAL SIIEAYP
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYP 356

[Close]

BLAST of Moc02g05940 vs. ExPASy TrEMBL
Match: A0A6J1G506 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111450978 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 8.0e-165
Identity = 278/355 (78.31%), Postives = 322/355 (90.70%), Query Frame = 0


Query: 2   EAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGSPITLSQIASSIA 61
           E +E +ETE  L+GQA+IW+YMLCFADSMALK AV+L LADIINSHGSPI+LSQIASSI 
Sbjct: 5   ETEETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIV 64

Query: 62  SSS--ASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPMI 121
           +S   A+P ISYL+RIMRLLVRRNIFA HH SDGG+ LYGLTHSS+WLLRD++LTLAPM+
Sbjct: 65  ASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMV 124

Query: 122 LAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVI 181
           LAELH W+VDPW CF++GVKEGGD FQKANG DIW FAS NPQFNQLFN+AMASTSKV++
Sbjct: 125 LAELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIM 184

Query: 182 NAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTH 241
            AILSVY+DGF+S+ SL DVGGGIGG +SEIVKA+PH+KGIN+DLPHV+STA  Y GVTH
Sbjct: 185 KAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINFDLPHVISTAPPYQGVTH 244

Query: 242 VGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLNPEGEG 301
           +GGDMFE++PKADA+FMKWILHDWDDKECVRIL+NC+KAIPEKTGKVI+VEV+L  +G+G
Sbjct: 245 IGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDG 304

Query: 302 AFDDTRLYFDLLMLAHTNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIEAYP 355
           AFDDTR YFDLLMLAHTNG+ERTEKEWK ILEEAGF RY+++ LPA+TSIIEAYP
Sbjct: 305 AFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYP 359

[Close]

BLAST of Moc02g05940 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 263.5 bits (672), Expect = 2.5e-70
Identity = 149/371 (40.16%), Postives = 219/371 (59.03%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGS--PITLSQIAS 60
           ME  + K  + E +   DIW+Y+  FAD  A K A+ L++ + I +H S  P+TL++++S
Sbjct: 15  MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74

Query: 61  SIASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLL--RDSDLTLA 120
           ++   SASP  S+L RIMR LV + IF      DG  T Y  T  SR L+  R    +LA
Sbjct: 75  AV---SASP--SHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 134

Query: 121 PMILAELHHWLVDPWHCFAEGVKE--GGDI---FQKANGHDIWGFASKNPQFNQLFNDAM 180
           P +L E    ++ PW   +  V     G     F   +G D+W FA  NP  + + N+AM
Sbjct: 135 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 194

Query: 181 ASTSKVVINAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTA 240
           A  ++ V+  +       F+ V ++ DVGGG G  +  +VK +P +KG N+DLPHV+  A
Sbjct: 195 ACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 254

Query: 241 TAYPGVTHVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEV 300
               GV +V GDMF+S+P  DAIF+KW+LHDW DK+C++IL+NC++A+P   GKV+IVE 
Sbjct: 255 EVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVES 314

Query: 301 VLN--------PEGEGAFDDTRLYFDLLMLAHTN-GKERTEKEWKKILEEAGFRRYKLVT 354
           V+          E +   +  RL  D++M+AHT+ GKERT KEW  +L+EAGF RY++  
Sbjct: 315 VIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRD 374

[Close]

BLAST of Moc02g05940 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 246.1 bits (627), Expect = 4.2e-65
Identity = 132/319 (41.38%), Postives = 205/319 (64.26%), Query Frame = 0


Query: 30  MALKSAVQLQLADIINSHGSPITLSQIASSIASSSASPNISYLARIMRLLVRRNIFAAHH 89
           MALKSA++L L +I+  +GSP++ ++IAS + + +    +  L RI+RLL   ++    +
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPV-MLDRILRLLTSYSVLTCSN 93

Query: 90  ---PSDGGDTLYGLTHSSRWLLRDSD-LTLAPMILAELHHWLVDPWHCFAEGVKEGGDIF 149
                DG + +YGL    ++L ++ D +++A + L      L++ W+   + + +GG  F
Sbjct: 94  RKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPF 153

Query: 150 QKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKDGFNSVGSLADVGGGIGG 209
            KA G   + +   +P+FN++FN+ M++ S + +  IL  YK GF  + SL DVGGGIG 
Sbjct: 154 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYK-GFEGLTSLVDVGGGIGA 213

Query: 210 AVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFESVPKADAIFMKWILHDWDD 269
            +  IV  YP++KGIN+DLPHV+  A ++PG+ HVGGDMF SVPK DAIFMKWI HDW D
Sbjct: 214 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSD 273

Query: 270 KECVRILENCRKAIPEKTGKVIIVEVVLNPEGEGAFDDTR--LYFDLLMLAHT-NGKERT 329
           + CV+ L+NC +++PE  GKVI+ E +L PE   +   T+  ++ D +MLAH   GKERT
Sbjct: 274 EHCVKFLKNCYESLPE-DGKVILAECIL-PETPDSSLSTKQVVHVDCIMLAHNPGGKERT 333

Query: 330 EKEWKKILEEAGFRRYKLV 342
           EKE++ + + +GF+  K+V
Sbjct: 334 EKEFEALAKASGFKGIKVV 348

[Close]

BLAST of Moc02g05940 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 235.0 bits (598), Expect = 9.6e-62
Identity = 137/364 (37.64%), Postives = 202/364 (55.49%), Query Frame = 0


Query: 1   MEAKEAKETEAELRGQADIWRYMLCFADSMALKSAVQLQLADIINSHGS--PITLSQIAS 60
           ME  +    + E +   DIWRY+  FAD  A K A+ L++ + I +H S  P+TLS+++S
Sbjct: 1   MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60

Query: 61  SIASSSASPNISYLARIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSRWLLRDSDLTLAPM 120
           ++   SASP  S+L RIMR LV + +F      DG  T Y  T  SR            M
Sbjct: 61  AV---SASP--SHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSR-----------RM 120

Query: 121 ILAELHHWLVDPWHCFAEGVKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVV 180
           ++ +LH                         G D+W FA  N   +QL N+AMA  ++ V
Sbjct: 121 MITKLH-------------------------GKDLWAFAQDNLCHSQLINEAMACDARRV 180

Query: 181 INAILSVYKDGFNSVGSLADVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVT 240
           +  +    +  F+ V ++ DVGGG G  +  +VK +P +KG N+DLPHV+  A    GV 
Sbjct: 181 VPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVE 240

Query: 241 HVGGDMFESVPKADAIFMKWILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVLN---- 300
           +V GDMF+S+P +DA+ +KW+LHDW DK+C++IL+NC++A+    GKV+IVE V+     
Sbjct: 241 NVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKN 300

Query: 301 ----PEGEGAFDDTRLYFDLLMLAHTN-GKERTEKEWKKILEEAGFRRYKLVTLPALTSI 354
                E +   +  RL  D++M+ HT+ GKERT KEW  +L EAGF RY++     + S+
Sbjct: 301 TMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSL 323

[Close]

BLAST of Moc02g05940 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.8e-55
Identity = 128/337 (37.98%), Postives = 190/337 (56.38%), Query Frame = 0


Query: 30  MALKSAVQLQLADIINSHGSPITLS--QIASSIASSSASPNISYLA-RIMRLLVRRNIFA 89
           M LK+A +L + D I + G+   LS  +IA S+ +   +P    L  R++ LLV  +I  
Sbjct: 44  MVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILK 103

Query: 90  AHHPSDGG-------DTLYGLTHSSRWLLRDSDLT--LAPMILAELHHWLVDPWHCFAEG 149
                 G        + +Y      ++ LRDSD T  L P+ +          W    + 
Sbjct: 104 CRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDV 163

Query: 150 VKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKDGFNSVGSLA 209
           + EG D F  A+G  I+ + + +  F +LFN AM+  S +++  +L VY+ GF  V +L 
Sbjct: 164 ILEGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYR-GFEDVNTLV 223

Query: 210 DVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFESVPKADAIFMK 269
           DVGGG G  +  +   YPH+KG+N+DL  V++ A  YPGV HV GDMF  VPK DA+FMK
Sbjct: 224 DVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFMK 283

Query: 270 WILHDWDDKECVRILENCRKAIPEKTGKVIIVEVVL--NPEGEGAFDDTRLYFDLLMLAH 329
           WILHDW D++C++IL+NC K++PEK GK+IIVE V    P+G     +T    DLLML  
Sbjct: 284 WILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQ 343

Query: 330 -TNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIE 352
            + GKER+  +++ +   +GF R +++ L    S+IE
Sbjct: 344 CSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

[Close]

BLAST of Moc02g05940 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-54
Identity = 129/337 (38.28%), Postives = 201/337 (59.64%), Query Frame = 0


Query: 30  MALKSAVQLQLADII--NSHGSPITLSQIASSIASSSASPNISYLA-RIMRLLVRRNIF- 89
           M LK+A++L + D I   S+G+ ++ S+IA S+ +   +P    L  R++RLLV  +I  
Sbjct: 44  MVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILK 103

Query: 90  -AAHHPSDGGDT-----LYGLTHSSRWLLRDSD--LTLAPMILAELHHWLVDPWHCFAEG 149
                  + G T     +Y      ++ L+DSD   +L+ ++L      ++  W    + 
Sbjct: 104 CCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDV 163

Query: 150 VKEGGDIFQKANGHDIWGFASKNPQFNQLFNDAMASTSKVVINAILSVYKDGFNSVGSLA 209
           + EG D F  A+   ++ + S + QF++LF+ AM+ +S +V+  +L  Y+ GF  V +L 
Sbjct: 164 ILEGKDAFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEEYR-GFEDVNTLV 223

Query: 210 DVGGGIGGAVSEIVKAYPHMKGINYDLPHVVSTATAYPGVTHVGGDMFESVPKADAIFMK 269
           DVGGGIG  +  I   YPH+KG+N+DL  V++ A  YPGV HV GDMF  VPK DAIFMK
Sbjct: 224 DVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIFMK 283

Query: 270 WILHDWDDKECVRILENCRKAIPEKTGKVIIVEVV--LNPEGEGAFDDTRLYFDLLMLAH 329
           WILHDW D++C++IL+NC K++PEK GKVIIVE++  + P+      +T L  DLLML  
Sbjct: 284 WILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEMITPMEPKPNDFSCNTVLGMDLLMLTQ 343

Query: 330 -TNGKERTEKEWKKILEEAGFRRYKLVTLPALTSIIE 352
            + GKER+  +++ +   +GF   +++ L    S+IE
Sbjct: 344 CSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIE 378

The following BLAST results are available for this feature:

BLAST of Moc02g05940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140296.1	2.3e-203	100.00	xanthohumol 4'-O-methyltransferase-like [Momordica charantia]	[more]
XP_022140297.1	3.0e-182	89.83	xanthohumol 4'-O-methyltransferase-like [Momordica charantia]	[more]
XP_038902222.1	2.3e-166	81.23	xanthohumol 4-O-methyltransferase-like [Benincasa hispida]	[more]
XP_008448276.2	1.5e-165	80.90	PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo] >KAA006210...	[more]
XP_022946951.1	1.6e-164	78.31	xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata]	[more]

BLAST of Moc02g05940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB56	1.2e-112	55.83	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]
B0ZB55	8.9e-105	54.18	Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P...	[more]
Q6WUC2	2.2e-103	52.57	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
Q9LEL6	1.8e-81	44.61	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1	[more]
Q5C9L7	2.9e-79	42.03	(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=...	[more]

BLAST of Moc02g05940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CFN9	1.1e-203	100.00	xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1CGG0	1.4e-182	89.83	xanthohumol 4'-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5A7V8T5	7.2e-166	80.90	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BJA7	7.2e-166	80.90	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490...	[more]
A0A6J1G506	8.0e-165	78.31	xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Moc02g05940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	2.5e-70	40.16	O-methyltransferase family protein	[more]
AT5G54160.1	4.2e-65	41.38	O-methyltransferase 1	[more]
AT4G35150.1	9.6e-62	37.64	O-methyltransferase family protein	[more]
AT1G77520.1	1.8e-55	37.98	O-methyltransferase family protein	[more]
AT1G77530.1	1.1e-54	38.28	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 109..355 e-value: 7.4E-86 score: 289.3
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 29..353 e-value: 1.6E-95 score: 317.3
None	No IPR available	PANTHER	PTHR11746:SF235	(R,S)-RETICULINE 7-O-METHYLTRANSFERASE-LIKE ISOFORM X1	coord: 5..354
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 131..336 e-value: 2.5E-66 score: 223.0
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..355 e-value: 1.1E-128 score: 427.1
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 5..354
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 20..354 score: 81.80809
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 30..80 e-value: 1.5E-13 score: 50.5
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 2..107 e-value: 2.2E-33 score: 116.0
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 9..111
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 113..353

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g05940.1	Moc02g05940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0050630	(iso)eugenol O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0102719	S-adenosyl-L-methionine:eugenol-O-methyltransferase activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0008171	O-methyltransferase activity