Homology
BLAST of Moc02g05580 vs. NCBI nr
Match:
XP_022140614.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])
HSP 1 Score: 1071.2 bits (2769), Expect = 3.2e-309
Identity = 542/595 (91.09%), Postives = 543/595 (91.26%), Query Frame = 0
Query: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL
Sbjct: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
Query: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSI 120
IPLVSYEHLKPYIDRISSGDDSPILCAHPIT+
Sbjct: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITD---------------------------- 120
Query: 121 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYT 180
FARAESVTKGGIVARSASTSLSRSSDFNNMESGSYT
Sbjct: 121 ------------------------FARAESVTKGGIVARSASTSLSRSSDFNNMESGSYT 180
Query: 181 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 240
SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT
Sbjct: 181 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 240
Query: 241 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 300
GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS
Sbjct: 241 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 300
Query: 301 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 360
MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR
Sbjct: 301 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 360
Query: 361 ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK 420
ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK
Sbjct: 361 ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK 420
Query: 421 NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE 480
NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE
Sbjct: 421 NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE 480
Query: 481 EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA 540
EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA
Sbjct: 481 EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA 540
Query: 541 SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 596
SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR
Sbjct: 541 SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 543
BLAST of Moc02g05580 vs. NCBI nr
Match:
XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])
HSP 1 Score: 958.7 bits (2477), Expect = 2.3e-275
Identity = 467/583 (80.10%), Postives = 514/583 (88.16%), Query Frame = 0
Query: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
MSNLDEKDKQ LQ+LEDVT ADEIQRRVL EILSTNAHVEYL+QHGL+GSTD STFKNL
Sbjct: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
Query: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSI 120
IP+VSYEHLKPYIDRI +GDDSPILCAHPITEF S+GTSGGEQKL P EQE R+LSI
Sbjct: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
Query: 121 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNME-SGSY 180
N MRP++K+LFP L+NGKEMFF+FA+ ES+TK GIVARS STS +S NN++ SY
Sbjct: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
Query: 181 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 240
TSP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIR
Sbjct: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
Query: 241 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 300
TGT+N KI D VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTG
Sbjct: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
Query: 301 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 360
SMS YIPQLNYY LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVD
Sbjct: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
Query: 361 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 420
R N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV R
Sbjct: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
Query: 421 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFK 480
KNV+LSLD EKTDEAELHNAV G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FK
Sbjct: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
Query: 481 EEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQG 540
EEISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER QG
Sbjct: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
Query: 541 ASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 583
ASISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT H
Sbjct: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
BLAST of Moc02g05580 vs. NCBI nr
Match:
XP_022140368.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia])
HSP 1 Score: 870.9 bits (2249), Expect = 6.2e-249
Identity = 425/574 (74.04%), Postives = 497/574 (86.59%), Query Frame = 0
Query: 4 LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63
LD KDKQ+L ++ED TSNADEIQRRV+ EILS NAHVEYLKQ GLDGSTD+STFK LIP+
Sbjct: 6 LDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKLIPV 65
Query: 64 VSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNC 123
VSYE LKPYIDRI+ GDDSPILCA IT+F IS+GTSGG QKL PMF+ EL+RRL+
Sbjct: 66 VSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLATNYY 125
Query: 124 MRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNME-SGSYTSP 183
P +K+LFP L NGKEMFF+F RAE+ TKGG+VA+ S+ LS+ S F+++E +YTSP
Sbjct: 126 AIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAYTSP 185
Query: 184 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 243
+IIRCTD YQSLYCQLLCGL Q + V+ +GALFASTL+Y+ KFLENHW LL NDIRTGT
Sbjct: 186 IDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIRTGT 245
Query: 244 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 303
V+ KI D LVRESVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ +VTGSMS
Sbjct: 246 VDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTGSMS 305
Query: 304 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 363
QYIPQLN+Y C P++S+FYASSEC+FGINLDPL KPEEISYTF+PTMAYFEFLPVDRAN
Sbjct: 306 QYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVDRAN 365
Query: 364 ANR-EVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN 423
+N EVGQELVDLVDVKLGQEYEV+VTTY GLYRYC+GDI+RVTGFK K P FSF CRKN
Sbjct: 366 SNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFACRKN 425
Query: 424 VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 483
V+LSLD EKTDE +LH+AV A LL+P GA I DYTSYAD+STFPGHYVLYWEI +EE
Sbjct: 426 VILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQCREE 485
Query: 484 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 543
S IPS++YEDCCLAVEESL+S+YRQARS+ KVINPLEIK+VE GTF+KL+E+A +GAS
Sbjct: 486 TSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDRGAS 545
Query: 544 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPK 576
ISQYKTPRC+ STQ++ELLD+KVV++YFS++YPK
Sbjct: 546 ISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578
BLAST of Moc02g05580 vs. NCBI nr
Match:
KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 812.8 bits (2098), Expect = 2.0e-231
Identity = 396/584 (67.81%), Postives = 479/584 (82.02%), Query Frame = 0
Query: 5 DEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLV 64
DE+DKQ LQ++EDVT +A +IQR++L +ILSTNA VEYL+QHGL GST++STFKNLIPL+
Sbjct: 10 DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69
Query: 65 SYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCM 124
SYE L+PYIDRI++GDDSPILC +PITEF ISSGTSGGE KL PM+E+E +RRLS + +
Sbjct: 70 SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129
Query: 125 RPKIKELFPS--LYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTSP 184
P++K+LFP+ + GK M F FA+ E T+ GIVA +A+TSL + ++ NN TSP
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN------TSP 189
Query: 185 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 244
+II CTD QSLYCQLLCGL Q + V +GALFASTL++V KFLE+HWV+LA+DIR GT
Sbjct: 190 KDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAGT 249
Query: 245 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 304
+NTKITD VRE+VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INAIVTGSM+
Sbjct: 250 LNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSMA 309
Query: 305 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 364
QY+P+LNYYSN LP++S Y SEC+ G+NLDPL P+ SYT IPTMAYFEFLP+DR
Sbjct: 310 QYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTK 369
Query: 365 ANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNV 424
++RE+ +ELVDLVDVKLG EYE+++TTY GLYRYC+GDI+RVTGFK KAP FSFVCRKNV
Sbjct: 370 SSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPNFSFVCRKNV 429
Query: 425 VLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEI 484
VLSLD EKTDEAELH+AV+ A LL+PFGA IADYTS+AD+S PGHYVLYWEIH K I
Sbjct: 430 VLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGGI 489
Query: 485 SIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASI 544
S IPSSIYEDCC A+EE LN YR RSH+K INPLEIK+V+ GTF+KL+ +A +GAS+
Sbjct: 490 S-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASM 549
Query: 545 SQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 587
SQYKTPRCV ST I+ELLD+ VV YFSK+ PK D HRL +
Sbjct: 550 SQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586
BLAST of Moc02g05580 vs. NCBI nr
Match:
XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])
HSP 1 Score: 806.6 bits (2082), Expect = 1.4e-229
Identity = 395/584 (67.64%), Postives = 478/584 (81.85%), Query Frame = 0
Query: 5 DEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLV 64
DE+DKQ LQ++EDVT +A +IQR++L +ILSTNA VEYL+QHGL GST++STFKNLIPL+
Sbjct: 10 DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69
Query: 65 SYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCM 124
SYE L+PYIDRI++GDDSPILC +PITEF ISSGTSGGE KL PM+E+E +RRLS + +
Sbjct: 70 SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129
Query: 125 RPKIKELFPS--LYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTSP 184
P++K+LFP+ + GK M F FA+ E T+ GIVA +A+TSL + ++ NN TSP
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN------TSP 189
Query: 185 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 244
+II CTD QSLYCQLLCGL Q + V +GALFASTL++V KFLE+HWV+LA+DIR GT
Sbjct: 190 KDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAGT 249
Query: 245 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 304
+NTKITD VRE+VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INAIVTGSM+
Sbjct: 250 LNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSMA 309
Query: 305 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 364
QY+P+LNYYSN LP++S Y +SEC+ G+NLDPL P+ SYT IPTMAYFEFLP+DR
Sbjct: 310 QYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTK 369
Query: 365 ANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNV 424
++RE+ +ELVDLVDVKLG EYE+++TTY GLYRY +GDI+ VTGFK KAP FSFVCRKNV
Sbjct: 370 SSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPNFSFVCRKNV 429
Query: 425 VLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEI 484
VLSLD EKTDEAELH+AV+ A LL+PFGA IADYTS+AD+S PGHYVLYWEIH K I
Sbjct: 430 VLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGGI 489
Query: 485 SIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASI 544
S IPSSIYEDCC A+EE LN YR RSH+K INPLEIK+V+ GTF+KL+ +A +GAS+
Sbjct: 490 S-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASM 549
Query: 545 SQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 587
SQYKTPRCV ST I+ELLD+ VV YFSKK PK D HRL +
Sbjct: 550 SQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586
BLAST of Moc02g05580 vs. ExPASy Swiss-Prot
Match:
Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)
HSP 1 Score: 704.9 bits (1818), Expect = 7.7e-202
Identity = 349/590 (59.15%), Postives = 443/590 (75.08%), Query Frame = 0
Query: 3 NLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIP 62
+L EK+K LQ +EDVT+NAD++QRRVL EILS NA VEYLK+HGL+G TD TFK+++P
Sbjct: 16 SLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMP 75
Query: 63 LVSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRN 122
+V+YE ++P I+RI++GD S +LC++PI+EF SSGTSGGE+KL P E+EL RR + +
Sbjct: 76 VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 135
Query: 123 CMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG---SY 182
+ P + + P L GK M+F F ++ES T GG+ AR TS +SS F N +Y
Sbjct: 136 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 195
Query: 183 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 242
TSPN+ I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS + KFLE HW LA DIR
Sbjct: 196 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 255
Query: 243 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 302
TGT++++ITD VRE+V ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ IVTG
Sbjct: 256 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 315
Query: 303 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 362
+MSQYIP L+YYSN LPL+ YASSECYFG+NL PL KP E+SYT IP MAYFEFLPV
Sbjct: 316 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 375
Query: 363 RANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFK 422
R + + QELVDLVDVKLGQEYE++VTTY GLYRY +GD++ V GFK
Sbjct: 376 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 435
Query: 423 YKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPG 482
AP FSF+CRKNVVLS+D +KTDE EL NAVK A L PF A++++YTSYAD S+ PG
Sbjct: 436 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 495
Query: 483 HYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTF 542
HYVL+WE+ + IP S++EDCCL +EESLNS+YRQ R K I PLEIKMVE+GTF
Sbjct: 496 HYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 555
Query: 543 DKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 579
DKL++ A GASI+QYKTPRCV I+ELL+++VV SYFS K PKW P
Sbjct: 556 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604
BLAST of Moc02g05580 vs. ExPASy Swiss-Prot
Match:
O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)
HSP 1 Score: 698.7 bits (1802), Expect = 5.5e-200
Identity = 346/589 (58.74%), Postives = 442/589 (75.04%), Query Frame = 0
Query: 4 LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63
LD+K+KQ LQ +E++TSNAD++QR+VL EIL+ NA VEYL++H L+G TD TFKN++P+
Sbjct: 17 LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76
Query: 64 VSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNC 123
++YE ++P I+RI++GD SPIL + PI+EF SSGTSGGE+KL P E+EL RR + +
Sbjct: 77 ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136
Query: 124 MRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG---SYT 183
+ P + + P L NGK M+F F ++ES T GG+ AR TS +SS F +YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196
Query: 184 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 243
SPNE I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS + KFLE HW+ L DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256
Query: 244 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 303
GT+++ ITD VRE+V ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ IVTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316
Query: 304 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 363
MSQYIP L+YYSN LPL+ YASSECYFG+NL PL KP E+SYT IP+MAYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376
Query: 364 ANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKY 423
N + QELVDLVDVKLGQEYE++VTTY GL RY +GD++RVTGFK
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436
Query: 424 KAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGH 483
KAP FSF+CRKNVVLS+D +KTDE EL NAVK A L PF A++++YTSYAD S+ PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496
Query: 484 YVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFD 543
YVL+WE+ + + IP S++EDCCLAVEES N++YRQ R K I PLEIK+VE GTFD
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556
Query: 544 KLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 579
KL++ A GASI+QYKTPRCV I+ELL+++VV SYFS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604
BLAST of Moc02g05580 vs. ExPASy Swiss-Prot
Match:
Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)
HSP 1 Score: 634.4 bits (1635), Expect = 1.3e-180
Identity = 313/574 (54.53%), Postives = 414/574 (72.13%), Query Frame = 0
Query: 10 QVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTD-VSTFKNLIPLVSYEH 69
+ L+ +E VT+NA ++QRRVL EIL+ NA EYL+++G+ GS D V F+ L+PLV+YE
Sbjct: 22 EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81
Query: 70 LKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCMRPKI 129
L+P I RI++GD SPI PI+EF SSGTSGGE+KL P E+ RR + + + P +
Sbjct: 82 LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141
Query: 130 KELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG---SYTSPNEI 189
+ L GK M+ F +AES T GG+ AR TS RS F + SYTSP+E
Sbjct: 142 SQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEA 201
Query: 190 IRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGTVNT 249
I C D+YQS+Y QLLCGL+ R V+ +GA+FAS + FLE HW L +DIRTG ++
Sbjct: 202 ILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDP 261
Query: 250 KITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMSQYI 309
+ITD++VR++V +L+ DP LAD IE EC++ +WEGI+ RLWP TK+I+ IVTG+MSQYI
Sbjct: 262 EITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYI 321
Query: 310 PQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRANANR 369
P L +Y LPL YASSECYFG+NL+P+ KP +++YT IPTM Y+EFLPV+ NA
Sbjct: 322 PTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATA 381
Query: 370 EVG-QELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNVVL 429
E ++LVDLVDVKLG EYE++VTTY+GLYRY +GD++RV GFK KAP FSFV R+NV L
Sbjct: 382 EASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVAL 441
Query: 430 SLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEISI 489
S+D +KTDE ELH AV GA + L PFGA++ +YTSYAD +T PGHYVL+WE+ + +
Sbjct: 442 SVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWEL--RAGSTA 501
Query: 490 IPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASISQ 549
+P+S++E+CCL+VEE+LNS+YRQ R+ + I PLEI++V GTFDKL++ A +GASI+Q
Sbjct: 502 VPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQ 561
Query: 550 YKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 579
YK PRCV +VELLD +V YFS K PKW P
Sbjct: 562 YKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSP 593
BLAST of Moc02g05580 vs. ExPASy Swiss-Prot
Match:
O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)
HSP 1 Score: 599.4 bits (1544), Expect = 4.6e-170
Identity = 299/582 (51.37%), Postives = 402/582 (69.07%), Query Frame = 0
Query: 6 EKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLVS 65
EKD + L+ +E++T NAD +Q +LAEIL+ NA EYL++ L G+TD TFK IP+++
Sbjct: 17 EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76
Query: 66 YEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCMR 125
YE L+P I RI+ GD SPIL AHPI+EF SSGTS GE+KL P ++EL RR + + +
Sbjct: 77 YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
Query: 126 PKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNM---ESGSYTSP 185
P + P L GK M+F F ++E+ T GG+ AR TS +S F + YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196
Query: 186 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 245
NE I C D++QS+Y Q+LCGLL R V+ +GA+FAS L+ +FL+ HW A+DI G
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256
Query: 246 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 305
++++ITD +R+ + ILKPDP LA+FI EC WE I+TR+WPNTK+++ IVTG+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316
Query: 306 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 365
QYIP L YYS LP+ YASSECYFG+NL+P+SKP E+SYT +P MAYFEF+P+
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG--- 376
Query: 366 ANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNV 425
G + V+LVDV +G+EYE++VTTY GL RY +GDI+RVTGF AP F FV RKNV
Sbjct: 377 -----GTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436
Query: 426 VLSLDFEKTDEAELHNAVKGAGELLN-PFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 485
+LS+D +KTDE+EL AV+ A +L+ G+ +A+YTSYAD ST PGHYVLYWE+ ++
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496
Query: 486 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 545
CCL +EESLNS+YRQ+R + PLEI++V GTF++L++ A +GAS
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556
Query: 546 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHR 584
I+QYK PRCV T IVELLD++VV+++FS P W P R R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRRR 590
BLAST of Moc02g05580 vs. ExPASy Swiss-Prot
Match:
Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)
HSP 1 Score: 597.0 bits (1538), Expect = 2.3e-169
Identity = 304/580 (52.41%), Postives = 404/580 (69.66%), Query Frame = 0
Query: 9 KQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVS--TFKNLIPLVSY 68
++ L+++E VT NA + QRRVL EIL+ NA EYL++ G+ G + F+ L PLV+Y
Sbjct: 24 REKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLVTY 83
Query: 69 EHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCMRP 128
E + P + RI++GD SPIL P++EF SSGTSGGE+KL P E+E+ RR + + + P
Sbjct: 84 EDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLLMP 143
Query: 129 KIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGS---YTSPN 188
+ P L GK M+ +F ++E T GG+ AR TS RS F YTSP+
Sbjct: 144 VMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTSPD 203
Query: 189 EIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGTV 248
E + C DAYQS+Y QL+CGL+ R V+ +GA+FAS + +FLE HW L DIR G +
Sbjct: 204 EAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAGEL 263
Query: 249 NTKITDQLVRESVMNILK-PDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 308
+ +TD VR +V +L+ DP LAD IE+EC++ +W+GI+ R+WP+TK+I+ IVTG+M+
Sbjct: 264 DGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTGAMA 323
Query: 309 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 368
QYIP L +Y LPL YASSECYFG+NL+P+ KP E++YT IPTM YFEFLPV+ +
Sbjct: 324 QYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVN-SG 383
Query: 369 ANREVGQE-----LVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFV 428
AN E LVDLVDVKLG EYE++VTTY+GLYRY +GD++RV GFK AP F+FV
Sbjct: 384 ANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMFAFV 443
Query: 429 CRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADN-STFPGHYVLYWEI 488
RKNV LS+D +KTDEAELH AV A + L PFGA++ +YTSYAD +T PGHYVL+WE+
Sbjct: 444 RRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWEL 503
Query: 489 HFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERAT 548
+ +P+S++EDCCLAVEE LNS+YRQ R+ + I PLEI++V GTFDKL++ A
Sbjct: 504 RSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGTFDKLMDYAL 563
Query: 549 YQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKW 577
+GASI+QYK PRCV +VELLD +V A+YFS K PKW
Sbjct: 564 SRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKW 602
BLAST of Moc02g05580 vs. ExPASy TrEMBL
Match:
A0A6J1CG61 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011222 PE=3 SV=1)
HSP 1 Score: 1071.2 bits (2769), Expect = 1.5e-309
Identity = 542/595 (91.09%), Postives = 543/595 (91.26%), Query Frame = 0
Query: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL
Sbjct: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
Query: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSI 120
IPLVSYEHLKPYIDRISSGDDSPILCAHPIT+
Sbjct: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITD---------------------------- 120
Query: 121 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYT 180
FARAESVTKGGIVARSASTSLSRSSDFNNMESGSYT
Sbjct: 121 ------------------------FARAESVTKGGIVARSASTSLSRSSDFNNMESGSYT 180
Query: 181 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 240
SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT
Sbjct: 181 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 240
Query: 241 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 300
GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS
Sbjct: 241 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 300
Query: 301 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 360
MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR
Sbjct: 301 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 360
Query: 361 ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK 420
ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK
Sbjct: 361 ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRK 420
Query: 421 NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE 480
NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE
Sbjct: 421 NVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKE 480
Query: 481 EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA 540
EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA
Sbjct: 481 EISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGA 540
Query: 541 SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 596
SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR
Sbjct: 541 SISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFALCQYPYLMR 543
BLAST of Moc02g05580 vs. ExPASy TrEMBL
Match:
A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)
HSP 1 Score: 958.7 bits (2477), Expect = 1.1e-275
Identity = 467/583 (80.10%), Postives = 514/583 (88.16%), Query Frame = 0
Query: 1 MSNLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNL 60
MSNLDEKDKQ LQ+LEDVT ADEIQRRVL EILSTNAHVEYL+QHGL+GSTD STFKNL
Sbjct: 1 MSNLDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNL 60
Query: 61 IPLVSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSI 120
IP+VSYEHLKPYIDRI +GDDSPILCAHPITEF S+GTSGGEQKL P EQE R+LSI
Sbjct: 61 IPIVSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSI 120
Query: 121 RNCMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNME-SGSY 180
N MRP++K+LFP L+NGKEMFF+FA+ ES+TK GIVARS STS +S NN++ SY
Sbjct: 121 SNYMRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSY 180
Query: 181 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 240
TSP +I+ CTD YQSLYCQLLCGL Q +KVV +G+LFASTL+Y+ KFLENHWVLLANDIR
Sbjct: 181 TSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIR 240
Query: 241 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 300
TGT+N KI D VR+SVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINA+VTG
Sbjct: 241 TGTINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTG 300
Query: 301 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 360
SMS YIPQLNYY LPLI++FYASSEC+FGINLDPL KPEEISYTFIPT+AYFEFLPVD
Sbjct: 301 SMSHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVD 360
Query: 361 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 420
R N + EVGQE VDLVDVKLGQEYE++VTTY GLYRYCIGDIVRVTGFK KAP F FV R
Sbjct: 361 RTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGR 420
Query: 421 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFK 480
KNV+LSLD EKTDEAELHNAV G LL+ FGA + DYTSYAD+STFPGHYVLYWEI FK
Sbjct: 421 KNVILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFK 480
Query: 481 EEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQG 540
EEISIIPSSIYEDCCLA+EESL+S+YR+ARSHQKVINPLEIK+VEA TFDKL+ER QG
Sbjct: 481 EEISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQG 540
Query: 541 ASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 583
ASISQYKTPRCV STQI+E LD+KVVA+YFSKKYPKWDPT H
Sbjct: 541 ASISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583
BLAST of Moc02g05580 vs. ExPASy TrEMBL
Match:
A0A6J1CHU9 (indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 GN=LOC111011061 PE=3 SV=1)
HSP 1 Score: 870.9 bits (2249), Expect = 3.0e-249
Identity = 425/574 (74.04%), Postives = 497/574 (86.59%), Query Frame = 0
Query: 4 LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63
LD KDKQ+L ++ED TSNADEIQRRV+ EILS NAHVEYLKQ GLDGSTD+STFK LIP+
Sbjct: 6 LDAKDKQILYHIEDFTSNADEIQRRVITEILSKNAHVEYLKQLGLDGSTDISTFKKLIPV 65
Query: 64 VSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNC 123
VSYE LKPYIDRI+ GDDSPILCA IT+F IS+GTSGG QKL PMF+ EL+RRL+
Sbjct: 66 VSYEQLKPYIDRIADGDDSPILCADTITDFLISTGTSGGAQKLIPMFQSELLRRLATNYY 125
Query: 124 MRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNME-SGSYTSP 183
P +K+LFP L NGKEMFF+F RAE+ TKGG+VA+ S+ LS+ S F+++E +YTSP
Sbjct: 126 AIPMMKQLFPGLLNGKEMFFNFTRAETKTKGGMVAQLLSSCLSKCSLFSSVEVLRAYTSP 185
Query: 184 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 243
+IIRCTD YQSLYCQLLCGL Q + V+ +GALFASTL+Y+ KFLENHW LL NDIRTGT
Sbjct: 186 IDIIRCTDVYQSLYCQLLCGLCQNQIVLRVGALFASTLIYIFKFLENHWALLVNDIRTGT 245
Query: 244 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 303
V+ KI D LVRESVM++LKP+P+LADFIESECSKG WEGIVTRLWPNTK+I+ +VTGSMS
Sbjct: 246 VDAKIEDPLVRESVMSVLKPNPELADFIESECSKGAWEGIVTRLWPNTKYISTVVTGSMS 305
Query: 304 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 363
QYIPQLN+Y C P++S+FYASSEC+FGINLDPL KPEEISYTF+PTMAYFEFLPVDRAN
Sbjct: 306 QYIPQLNFYCKC-PIVSVFYASSECFFGINLDPLCKPEEISYTFMPTMAYFEFLPVDRAN 365
Query: 364 ANR-EVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKN 423
+N EVGQELVDLVDVKLGQEYEV+VTTY GLYRYC+GDI+RVTGFK K P FSF CRKN
Sbjct: 366 SNNGEVGQELVDLVDVKLGQEYEVVVTTYAGLYRYCMGDILRVTGFKNKTPNFSFACRKN 425
Query: 424 VVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 483
V+LSLD EKTDE +LH+AV A LL+P GA I DYTSYAD+STFPGHYVLYWEI +EE
Sbjct: 426 VILSLDIEKTDEVDLHHAVTEASGLLSPLGAIIVDYTSYADSSTFPGHYVLYWEIQCREE 485
Query: 484 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 543
S IPS++YEDCCLAVEESL+S+YRQARS+ KVINPLEIK+VE GTF+KL+E+A +GAS
Sbjct: 486 TSTIPSTVYEDCCLAVEESLSSVYRQARSNNKVINPLEIKIVETGTFEKLMEQAVDRGAS 545
Query: 544 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPK 576
ISQYKTPRC+ STQ++ELLD+KVV++YFS++YPK
Sbjct: 546 ISQYKTPRCLKSTQMIELLDSKVVSNYFSQEYPK 578
BLAST of Moc02g05580 vs. ExPASy TrEMBL
Match:
A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)
HSP 1 Score: 806.6 bits (2082), Expect = 7.0e-230
Identity = 395/584 (67.64%), Postives = 478/584 (81.85%), Query Frame = 0
Query: 5 DEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLV 64
DE+DKQ LQ++EDVT +A +IQR++L +ILSTNA VEYL+QHGL GST++STFKNLIPL+
Sbjct: 10 DERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELSTFKNLIPLI 69
Query: 65 SYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCM 124
SYE L+PYIDRI++GDDSPILC +PITEF ISSGTSGGE KL PM+E+E +RRLS + +
Sbjct: 70 SYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFMRRLSFFDYV 129
Query: 125 RPKIKELFPS--LYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGSYTSP 184
P++K+LFP+ + GK M F FA+ E T+ GIVA +A+TSL + ++ NN TSP
Sbjct: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN------TSP 189
Query: 185 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 244
+II CTD QSLYCQLLCGL Q + V +GALFASTL++V KFLE+HWV+LA+DIR GT
Sbjct: 190 KDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVVLASDIRAGT 249
Query: 245 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 304
+NTKITD VRE+VMNIL P+ QLADFIE+ECSKGTWEGI+TRLWPN K+INAIVTGSM+
Sbjct: 250 LNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYINAIVTGSMA 309
Query: 305 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 364
QY+P+LNYYSN LP++S Y +SEC+ G+NLDPL P+ SYT IPTMAYFEFLP+DR
Sbjct: 310 QYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTK 369
Query: 365 ANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNV 424
++RE+ +ELVDLVDVKLG EYE+++TTY GLYRY +GDI+ VTGFK KAP FSFVCRKNV
Sbjct: 370 SSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPNFSFVCRKNV 429
Query: 425 VLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEI 484
VLSLD EKTDEAELH+AV+ A LL+PFGA IADYTS+AD+S PGHYVLYWEIH K I
Sbjct: 430 VLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLYWEIHVKGGI 489
Query: 485 SIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASI 544
S IPSSIYEDCC A+EE LN YR RSH+K INPLEIK+V+ GTF+KL+ +A +GAS+
Sbjct: 490 S-IPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASM 549
Query: 545 SQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHRLFA 587
SQYKTPRCV ST I+ELLD+ VV YFSKK PK D HRL +
Sbjct: 550 SQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586
BLAST of Moc02g05580 vs. ExPASy TrEMBL
Match:
A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)
HSP 1 Score: 761.1 bits (1964), Expect = 3.4e-216
Identity = 374/581 (64.37%), Postives = 465/581 (80.03%), Query Frame = 0
Query: 4 LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63
LDEKDK+ LQ +ED+T+ ADEIQR++L EILS NA+VEYL++HGLD STD STFK LIPL
Sbjct: 9 LDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKLIPL 68
Query: 64 VSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNC 123
V Y+ L+PYI RI+ GDDS ILC++PITEF SSGTSG E KL PM+EQE VRRLS +
Sbjct: 69 VYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSFFSY 128
Query: 124 MRPKIKELFPSL--YNGKEMFFHFARAESVTKGGIVARSASTSL-SRSSDFNNMESGSYT 183
+ P++K+LFP++ + K + FHFA+ E TKGGIV RS T+L RSS+ +M SG+ T
Sbjct: 129 ITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSGNNT 188
Query: 184 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 243
SP++II CTD+YQSLYCQLLCGL Q + V + ALFASTL++V KFLENHW+ LA DIRT
Sbjct: 189 SPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATDIRT 248
Query: 244 GTVNTKITDQLVRESVMNI-LKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 303
T+N KIT VRESVM I +KP+P++AD IE+EC KG WEGI+TRLWPN K+INA+VTG
Sbjct: 249 RTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAVVTG 308
Query: 304 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 363
SMSQYIP LNYY+N LP++S Y SSEC+ G+NLDPL P+E+SYT IPTMAYFEFLP+D
Sbjct: 309 SMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMAYFEFLPID 368
Query: 364 RANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCR 423
N EV Q LVDLVDVKLG EYE+++TT+ GLYRYC+GD+VRVTGFK KAP FSFV R
Sbjct: 369 MLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKAPNFSFVRR 428
Query: 424 KNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHF- 483
KNVVL++ EKTDEA LH AV+ G ++ FGATI DYT+YAD ST PGHYVLYWE+
Sbjct: 429 KNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYVLYWELTLD 488
Query: 484 ---KEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERA 543
++ ++ IP S++EDCCLA+E SLN LYR ARSH+K INPLEIK+V+AGTF+KL++ A
Sbjct: 489 DLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKLMQLA 548
Query: 544 TYQGASISQYKTPRCVTSTQ--IVELLDTKVVASYFSKKYP 575
+GASI+QYKTPRC+ S+Q I++LL++ +V++YFSK YP
Sbjct: 549 LNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYP 589
BLAST of Moc02g05580 vs. TAIR 10
Match:
AT5G54510.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 704.9 bits (1818), Expect = 5.5e-203
Identity = 349/590 (59.15%), Postives = 443/590 (75.08%), Query Frame = 0
Query: 3 NLDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIP 62
+L EK+K LQ +EDVT+NAD++QRRVL EILS NA VEYLK+HGL+G TD TFK+++P
Sbjct: 16 SLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMP 75
Query: 63 LVSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRN 122
+V+YE ++P I+RI++GD S +LC++PI+EF SSGTSGGE+KL P E+EL RR + +
Sbjct: 76 VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 135
Query: 123 CMRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG---SY 182
+ P + + P L GK M+F F ++ES T GG+ AR TS +SS F N +Y
Sbjct: 136 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 195
Query: 183 TSPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIR 242
TSPN+ I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS + KFLE HW LA DIR
Sbjct: 196 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 255
Query: 243 TGTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTG 302
TGT++++ITD VRE+V ILKPDP+LADF+ESEC K +W+GI+TRLWPNTK+++ IVTG
Sbjct: 256 TGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTG 315
Query: 303 SMSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVD 362
+MSQYIP L+YYSN LPL+ YASSECYFG+NL PL KP E+SYT IP MAYFEFLPV
Sbjct: 316 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVH 375
Query: 363 RANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFK 422
R + + QELVDLVDVKLGQEYE++VTTY GLYRY +GD++ V GFK
Sbjct: 376 RNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFK 435
Query: 423 YKAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPG 482
AP FSF+CRKNVVLS+D +KTDE EL NAVK A L PF A++++YTSYAD S+ PG
Sbjct: 436 NNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 495
Query: 483 HYVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTF 542
HYVL+WE+ + IP S++EDCCL +EESLNS+YRQ R K I PLEIKMVE+GTF
Sbjct: 496 HYVLFWELCLNGN-TPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 555
Query: 543 DKLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 579
DKL++ A GASI+QYKTPRCV I+ELL+++VV SYFS K PKW P
Sbjct: 556 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604
BLAST of Moc02g05580 vs. TAIR 10
Match:
AT4G27260.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 698.7 bits (1802), Expect = 3.9e-201
Identity = 346/589 (58.74%), Postives = 442/589 (75.04%), Query Frame = 0
Query: 4 LDEKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPL 63
LD+K+KQ LQ +E++TSNAD++QR+VL EIL+ NA VEYL++H L+G TD TFKN++P+
Sbjct: 17 LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76
Query: 64 VSYEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNC 123
++YE ++P I+RI++GD SPIL + PI+EF SSGTSGGE+KL P E+EL RR + +
Sbjct: 77 ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136
Query: 124 MRPKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESG---SYT 183
+ P + + P L NGK M+F F ++ES T GG+ AR TS +SS F +YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196
Query: 184 SPNEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRT 243
SPNE I C+D+YQS+Y Q+LCGL Q ++V+ +GA+FAS + KFLE HW+ L DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256
Query: 244 GTVNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGS 303
GT+++ ITD VRE+V ILKP P+LADF+E EC K +W+GI+TRLWPNTK+++ IVTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316
Query: 304 MSQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR 363
MSQYIP L+YYSN LPL+ YASSECYFG+NL PL KP E+SYT IP+MAYFEFLPV R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376
Query: 364 ANA-----------NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKY 423
N + QELVDLVDVKLGQEYE++VTTY GL RY +GD++RVTGFK
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKN 436
Query: 424 KAPCFSFVCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGH 483
KAP FSF+CRKNVVLS+D +KTDE EL NAVK A L PF A++++YTSYAD S+ PGH
Sbjct: 437 KAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 496
Query: 484 YVLYWEIHFKEEISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFD 543
YVL+WE+ + + IP S++EDCCLAVEES N++YRQ R K I PLEIK+VE GTFD
Sbjct: 497 YVLFWELCL-DGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556
Query: 544 KLLERATYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDP 579
KL++ A GASI+QYKTPRCV I+ELL+++VV SYFS K PKW P
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604
BLAST of Moc02g05580 vs. TAIR 10
Match:
AT2G14960.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 599.4 bits (1544), Expect = 3.2e-171
Identity = 299/582 (51.37%), Postives = 402/582 (69.07%), Query Frame = 0
Query: 6 EKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLVS 65
EKD + L+ +E++T NAD +Q +LAEIL+ NA EYL++ L G+TD TFK IP+++
Sbjct: 17 EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76
Query: 66 YEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCMR 125
YE L+P I RI+ GD SPIL AHPI+EF SSGTS GE+KL P ++EL RR + + +
Sbjct: 77 YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
Query: 126 PKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNM---ESGSYTSP 185
P + P L GK M+F F ++E+ T GG+ AR TS +S F + YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196
Query: 186 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 245
NE I C D++QS+Y Q+LCGLL R V+ +GA+FAS L+ +FL+ HW A+DI G
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256
Query: 246 VNTKITDQLVRESVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMS 305
++++ITD +R+ + ILKPDP LA+FI EC WE I+TR+WPNTK+++ IVTG+M+
Sbjct: 257 LDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMA 316
Query: 306 QYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRAN 365
QYIP L YYS LP+ YASSECYFG+NL+P+SKP E+SYT +P MAYFEF+P+
Sbjct: 317 QYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG--- 376
Query: 366 ANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNV 425
G + V+LVDV +G+EYE++VTTY GL RY +GDI+RVTGF AP F FV RKNV
Sbjct: 377 -----GTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNV 436
Query: 426 VLSLDFEKTDEAELHNAVKGAGELLN-PFGATIADYTSYADNSTFPGHYVLYWEIHFKEE 485
+LS+D +KTDE+EL AV+ A +L+ G+ +A+YTSYAD ST PGHYVLYWE+ ++
Sbjct: 437 LLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDG 496
Query: 486 ISIIPSSIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGAS 545
CCL +EESLNS+YRQ+R + PLEI++V GTF++L++ A +GAS
Sbjct: 497 ARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGAS 556
Query: 546 ISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRHR 584
I+QYK PRCV T IVELLD++VV+++FS P W P R R
Sbjct: 557 INQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRRR 590
BLAST of Moc02g05580 vs. TAIR 10
Match:
AT1G59500.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 595.5 bits (1534), Expect = 4.7e-170
Identity = 290/580 (50.00%), Postives = 408/580 (70.34%), Query Frame = 0
Query: 6 EKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLVS 65
E + + L+ +E++T N D +Q +VL EILS N++ EYLK+ L+G+ D +FK+ +P+V
Sbjct: 18 ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77
Query: 66 YEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCMR 125
YE LK I RIS+GD SPIL +HPITEF SSGTS GE+KL P E+++ RR + N +
Sbjct: 78 YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137
Query: 126 PKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNMESGS-YTSPNE 185
P + P L GK ++F F ++ES T GG+ AR A TS +S F +S S YTSP E
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSDSVYTSPKE 197
Query: 186 IIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGTVN 245
I C D+ QS+Y Q+LCGLL R +V +GA+F S L+ FL+N+W L+ DI TGT++
Sbjct: 198 AILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDISTGTLS 257
Query: 246 TKITDQLVRESVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSMSQ 305
+KI D ++ + NIL KPD +LA+F+ CS+ WEGI+T++WPNTK+++ IVTG+M++
Sbjct: 258 SKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTGAMAE 317
Query: 306 YIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDRANA 365
YIP L YYS LP+ S+ YASSE YFGINL+P+ KP E+SYT P MAYFEFLP +
Sbjct: 318 YIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHNHDGD 377
Query: 366 NREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSFVCRKNVV 425
LV+L DV++G+EYE+++TTY GLYRY +GDI+RVTGF AP F F+ R+NV+
Sbjct: 378 GGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRENVL 437
Query: 426 LSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEIHFKEEIS 485
LS++ +KTDEA+L AV+ A LL G + +YTSYAD T PGHYV+YWE+ +++ +
Sbjct: 438 LSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSRDQSN 497
Query: 486 IIPS-SIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERATYQGASI 545
+PS + CCL +EESLN++YRQ+R K I PLEI++V+ GTF++L++ + +G+SI
Sbjct: 498 ALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISRGSSI 557
Query: 546 SQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCRH 583
+QYK PRCV+ T I++LLD++VV+++FS P W P RH
Sbjct: 558 NQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERRH 597
BLAST of Moc02g05580 vs. TAIR 10
Match:
AT4G37390.1 (Auxin-responsive GH3 family protein )
HSP 1 Score: 588.2 bits (1515), Expect = 7.5e-168
Identity = 295/586 (50.34%), Postives = 407/586 (69.45%), Query Frame = 0
Query: 6 EKDKQVLQNLEDVTSNADEIQRRVLAEILSTNAHVEYLKQHGLDGSTDVSTFKNLIPLVS 65
EKD + L+ +E++T N D +Q +VL EIL+ N++ EYLK+ LDG D TFK+ +P+V+
Sbjct: 18 EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77
Query: 66 YEHLKPYIDRISSGDDSPILCAHPITEFRISSGTSGGEQKLFPMFEQELVRRLSIRNCMR 125
YE LKP I RIS+GD SPIL +HPITEF SSGTS GE+KL P E++L RR + + +
Sbjct: 78 YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137
Query: 126 PKIKELFPSLYNGKEMFFHFARAESVTKGGIVARSASTSLSRSSDFNNM---ESGSYTSP 185
P + P L GK ++F F ++ES T GG+ AR TS +S F YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197
Query: 186 NEIIRCTDAYQSLYCQLLCGLLQREKVVGIGALFASTLVYVSKFLENHWVLLANDIRTGT 245
NE I C+D+ QS+Y Q+LCGLL R +V+ +GA+FAS L+ FL+N+W LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257
Query: 246 VNTKITDQLVRESVMNIL-KPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAIVTGSM 305
++++I D ++ + IL KPD +LA+F+ CS+ WEGI+T++WPNTK+++ IVTG+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317
Query: 306 SQYIPQLNYYSNCLPLISLFYASSECYFGINLDPLSKPEEISYTFIPTMAYFEFLPVDR- 365
+QYIP L YYS LP+ YASSE YFGINL P+ KP E+SYT +P MAYFEFLP +
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377
Query: 366 ----ANANREVGQELVDLVDVKLGQEYEVIVTTYTGLYRYCIGDIVRVTGFKYKAPCFSF 425
A A+ + LV+L +V++G+EYE+++TTY GLYRY +GDI+RVTGF AP F F
Sbjct: 378 GDGAAEASLD-ETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKF 437
Query: 426 VCRKNVVLSLDFEKTDEAELHNAVKGAGELLNPFGATIADYTSYADNSTFPGHYVLYWEI 485
+ RKNV+LS++ +KTDEAEL AV+ A L G + +YTSYA+ T PGHYV+YWE+
Sbjct: 438 IRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWEL 497
Query: 486 HFKEEISIIPS-SIYEDCCLAVEESLNSLYRQARSHQKVINPLEIKMVEAGTFDKLLERA 545
+++ + + S + CCL +EESLNS+YRQ+R K I PLEI++V GTF++L++ A
Sbjct: 498 LGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYA 557
Query: 546 TYQGASISQYKTPRCVTSTQIVELLDTKVVASYFSKKYPKWDPTCR 582
+GASI+QYK PRCV+ T I+ELLD++VV+++FS P W P R
Sbjct: 558 ISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022140614.1 | 3.2e-309 | 91.09 | indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia] | [more] |
| XP_022140778.1 | 2.3e-275 | 80.10 | indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia] | [more] |
| XP_022140368.1 | 6.2e-249 | 74.04 | indole-3-acetic acid-amido synthetase GH3.5-like [Momordica charantia] | [more] |
| KAG6570863.1 | 2.0e-231 | 67.81 | Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... | [more] |
| XP_022944596.1 | 1.4e-229 | 67.64 | indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9LSQ4 | 7.7e-202 | 59.15 | Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... | [more] |
| O81829 | 5.5e-200 | 58.74 | Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... | [more] |
| Q8LQM5 | 1.3e-180 | 54.53 | Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... | [more] |
| O82333 | 4.6e-170 | 51.37 | Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... | [more] |
| Q60EJ6 | 2.3e-169 | 52.41 | Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CG61 | 1.5e-309 | 91.09 | indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ... | [more] |
| A0A6J1CIS9 | 1.1e-275 | 80.10 | indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ... | [more] |
| A0A6J1CHU9 | 3.0e-249 | 74.04 | indole-3-acetic acid-amido synthetase GH3.5-like OS=Momordica charantia OX=3673 ... | [more] |
| A0A6J1FUU5 | 7.0e-230 | 67.64 | indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... | [more] |
| A0A5A7V3L5 | 3.4e-216 | 64.37 | Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=... | [more] |
