Moc02g05380 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g05380
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionAPO protein 4, mitochondrial
Locationchr2: 3961919 .. 3965195 (+)
RNA-Seq ExpressionMoc02g05380
SyntenyMoc02g05380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCAAAATAGGCGTCTGAGGAGGAAACGGTCAGCATGGTCATGGGATTTTCCATTTTCCAAGCTTGATCGCGTGTCGACTCCTGTTGCCGGCACGGTTCAAGCAGTTTTGAGATGGACGTGTGAGATGTGTCGTAATGAGAATGCAGGAGGGGCAACACCAACTAACTTTTTCGGCAAAATTAAAGTAGATAAAAGTAAGACATATATATTTGAGGTAATTTAAGAAAAAGGTAAAAATACGTATTATAAAGGAGATGCATGAGAATGGTTAGGTTGGAAAATAAAAAATATAAATTTTGGGACAGTAAAATATGGATAAGGTTGGGCCTGTTTGAATTCTATCTTGAAGTTTTGGATCGGGAGGAGTGGGCGATTGAGGATTGTGGTGGCTCTTCTTGGGAGTTGAGAAAATAGAAAATTGGGTTATCTCCGATGGGCCCAACTTTCTCTTCAATTGCGATGGGTTGGCCTTTTTAGCACTTCGAGGAAAAGAATTTTCTATTTTTTTTTCCTAAATTTCTTTTAATTTTATTTCTTCCTATACTTATCTCTATTGTCTCTCGTCCTCATCCATTTCCACCGCGTGATTGATTGGAACACTTTTGCCCTTAGTAAACAATAATATAACCATAAGAATCATATGAGTAATTAAATCAAAATTTCTTTTAGTATTAATTTTTTCTTTATCATTAGTCTTACCAACCAGTAAGCTAGTTTATTTTTTTGGTATGACATGGTGACGTTGTAGCTTGAAGGTGAGTCTTTTGAGGATTTCTCTTCTTGAAGACTTGCATGGAGTTTCATTTTTTATCTTCAAAGAAAAAATTTACTTAGACCAGAGATTTTTATTTTTTACTTTTATTTCCACCTATTCATTAAGAAATCAAGAGAAGAGGATGGCAATTGTTGAAGTTCACTCCTTTCAGCAACTTGGTAATTTTTTAAAATTATTACTTATGTTTTTTTTTCTTCTTTCATTTTTGTTTTACTTATTTTCAGCAGATCAAATGTTTTTTCTTTTATTTTCTTCATTCATTTTTGTTTATCCAACTTTTAAATTGTTGTGATCTCAACTATCTATTTTGGATGGAAATTTTTATTAGATCAAATCAAGATTTTGATTTCTATTTATTTTTTATTCCAATCAAACACGCAAAATAATTTCAAATCCATATGCTAAAAAAAAAGATATCAAATCCAGATCCTTCCAAAGCTGGTTCCAAACATATGGTAAAAGAAATAGAGGTAATCAAACCGGAAAAAACCCGGATTTTGAAGCAATGAATGGAGCTGATCGGACCGATTCGGATTTATATTAGTGAACCCGATTTACTGGTTCGGCTCGGTCGTTTTAGCCTTTTAGATGGGTAGAAGTAAACTACTGACCCCGACCGGTCCGCTTTGCCGATTACCTCTCAAGTCTGGTTGATTGTGGTCACTTGCGCTCGAGCCTTGGCGCCCTCTTTTTCCCCATCGATGTCTGGAACTGCAAAACTTGATTTGGATCTCACATTTCTGCAAATGAATTGTTTCGAAGCGTATGTTGTTCTTCTCATCTCTACGAATTTCTGTTAGTTGGTCCCTCTTGTCTTTGAAAGCTCTCTCGTCTCTCGAAATCTCATCCTCTGTCCCTTAAACCATCTCTCTTACTCGCCGTCTCAACCACTCGGCTCACGCTATGTTTTCTCAAATCTCCCAGAATTTGTATGCCTCCTTTTGGCTGCGATTGGATTAGGACTTCAATTTATGCTTTCAAGTTTGTGGCTTTTCTTTCTCTTAAGATTTGGGAAATGTTAGTGCTTGTCCCTTGATGACAACTATGGCTTTTGTGGCTCTCAGAAGGAAATTGGTTCAGAACGTTGTGCAGGAATTCATGCTACACCGGTGCTATAGCTCTAAGGTCGACCTTAAGAAACTCCGTCCAATGATTTCGAAGAGAATTGAAAATCGGGCGAAATTCTATCCTATAAATGCCATGATTCCAGTGGCACAACAAGTTCTTGAAGCTAGGGCAGTACTTATTCATGGAGTTTCCACTCTTCTTAAGGCATTTCCAATTGTCTCTTGCAAGTCAGTGTCCGTTGAACAAACTCTCTTTAATTTCTCATCTTTGTGTCATGATTTTACTTTGTTTTCTTTTCTTTTTCCTATCTTTTTACATAGTTTTTAATCTAAATTGAATATTCCTTGAACTCATCTGTGTATTGGGGTAGTACCATTTTTGTCAGAAATTCTGCGTGACCTTGATTTTCTGTTTCCTGACTAGTAGCAGATATAGGAAATTTCCTGTACATGAAGTGAGGTCAATGAATACAATAATATATATATATATTATCTAGTTCTTTATACGCGCTAGGACCGCATGTATAATCTGTTCTTTTTAAAATTTTGAGGCGTTGATGTTAATTCGTGTTTGTGAATGCTGCTTCTAAAACTCCTAGACTCATTGTGTAAGTCTGTTCTTTCTAATTTTAGATTTTCGTTGTTAACTGAATACATGTTTGTGAATTGTGCTTCTGAAACTCCTAGGATCATTTTGTAGATTTTGTCCAGAAGTATATGTCGGTGAGCAAGGTCATCTAATACGGAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATCGGGTTCATGAATGGATCGGAGGTGATTTGAATGATGTTCTTGTCCCAGTAGAAGCATTTCACCTCCATCGCATGTTCCAAGATGTTATTAAACATGATGAGAGATTCAACTTTGAACGTGTTCCAGCAGTTGCCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTGGGAATTCAGATGGTGGTGGCTCAGGCAGAGATGAATGGTTGTCAAGTCATGAGCTGATGCCTTTAGCAAATGTAACTCTCAAGGCATGGGAAACGCTTAGAACCGGTGTGCAGAAACTGTTAATGGTTTATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGGGTTTTTAAATATGAGAGTTGGCGAGGCTCCCACTTCTGGGAGAAGGCAAATGTAGACGATTTAGTTCCTCCAAATATTGTATGGCATCGGCGACCACAAGATCCTCCCATGCTTGTCGACGAAGGAAGGGATTATTATGGACATGTACCAGCAGTGGTGGCTCTTTGCACACAGGCCGGCGTCGTTGCACCATCTAAATATCATTGCATGATGAAAATTCAAGGCTTATCACCACGAACTCAACAAAATCTCTGA

mRNA sequence

ATGGGTCAAAATAGGCGTCTGAGGAGGAAACGGTCAGCATGGTCATGGGATTTTCCATTTTCCAAGCTTGATCGCGTGTCGACTCCTGTTGCCGGCACGGTTCAAGCAGTTTTGAGATGGACGTGTGAGATGTGTCGTAATGAGAATGCAGGAGGGGCAACACCAACTAACTTTTTCGGCAAAATTAAAGTAGATAAAAATTTGGGAAATGTTAGTGCTTGTCCCTTGATGACAACTATGGCTTTTGTGGCTCTCAGAAGGAAATTGGTTCAGAACGTTGTGCAGGAATTCATGCTACACCGGTGCTATAGCTCTAAGGTCGACCTTAAGAAACTCCGTCCAATGATTTCGAAGAGAATTGAAAATCGGGCGAAATTCTATCCTATAAATGCCATGATTCCAGTGGCACAACAAGTTCTTGAAGCTAGGGCAGTACTTATTCATGGAGTTTCCACTCTTCTTAAGGCATTTCCAATTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTCGGTGAGCAAGGTCATCTAATACGGAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATCGGGTTCATGAATGGATCGGAGGTGATTTGAATGATGTTCTTGTCCCAGTAGAAGCATTTCACCTCCATCGCATGTTCCAAGATGTTATTAAACATGATGAGAGATTCAACTTTGAACGTGTTCCAGCAGTTGCCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTGGGAATTCAGATGGTGGTGGCTCAGGCAGAGATGAATGGTTGTCAAGTCATGAGCTGATGCCTTTAGCAAATGTAACTCTCAAGGCATGGGAAACGCTTAGAACCGGTGTGCAGAAACTGTTAATGGTTTATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGGGTTTTTAAATATGAGAGTTGGCGAGGCTCCCACTTCTGGGAGAAGGCAAATGTAGACGATTTAGTTCCTCCAAATATTGTATGGCATCGGCGACCACAAGATCCTCCCATGCTTGTCGACGAAGGAAGGGATTATTATGGACATGTACCAGCAGTGGTGGCTCTTTGCACACAGGCCGGCGTCGTTGCACCATCTAAATATCATTGCATGATGAAAATTCAAGGCTTATCACCACGAACTCAACAAAATCTCTGA

Coding sequence (CDS)

ATGGGTCAAAATAGGCGTCTGAGGAGGAAACGGTCAGCATGGTCATGGGATTTTCCATTTTCCAAGCTTGATCGCGTGTCGACTCCTGTTGCCGGCACGGTTCAAGCAGTTTTGAGATGGACGTGTGAGATGTGTCGTAATGAGAATGCAGGAGGGGCAACACCAACTAACTTTTTCGGCAAAATTAAAGTAGATAAAAATTTGGGAAATGTTAGTGCTTGTCCCTTGATGACAACTATGGCTTTTGTGGCTCTCAGAAGGAAATTGGTTCAGAACGTTGTGCAGGAATTCATGCTACACCGGTGCTATAGCTCTAAGGTCGACCTTAAGAAACTCCGTCCAATGATTTCGAAGAGAATTGAAAATCGGGCGAAATTCTATCCTATAAATGCCATGATTCCAGTGGCACAACAAGTTCTTGAAGCTAGGGCAGTACTTATTCATGGAGTTTCCACTCTTCTTAAGGCATTTCCAATTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTCGGTGAGCAAGGTCATCTAATACGGAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATCGGGTTCATGAATGGATCGGAGGTGATTTGAATGATGTTCTTGTCCCAGTAGAAGCATTTCACCTCCATCGCATGTTCCAAGATGTTATTAAACATGATGAGAGATTCAACTTTGAACGTGTTCCAGCAGTTGCCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTGGGAATTCAGATGGTGGTGGCTCAGGCAGAGATGAATGGTTGTCAAGTCATGAGCTGATGCCTTTAGCAAATGTAACTCTCAAGGCATGGGAAACGCTTAGAACCGGTGTGCAGAAACTGTTAATGGTTTATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGGGTTTTTAAATATGAGAGTTGGCGAGGCTCCCACTTCTGGGAGAAGGCAAATGTAGACGATTTAGTTCCTCCAAATATTGTATGGCATCGGCGACCACAAGATCCTCCCATGCTTGTCGACGAAGGAAGGGATTATTATGGACATGTACCAGCAGTGGTGGCTCTTTGCACACAGGCCGGCGTCGTTGCACCATCTAAATATCATTGCATGATGAAAATTCAAGGCTTATCACCACGAACTCAACAAAATCTCTGA

Protein sequence

MGQNRRLRRKRSAWSWDFPFSKLDRVSTPVAGTVQAVLRWTCEMCRNENAGGATPTNFFGKIKVDKNLGNVSACPLMTTMAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLSPRTQQNL
Homology
BLAST of Moc02g05380 vs. NCBI nr
Match: XP_022140768.1 (APO protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-188
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 87  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 146
           RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL
Sbjct: 22  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 81

Query: 147 IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 206
           IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV
Sbjct: 82  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 141

Query: 207 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 266
           EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW
Sbjct: 142 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 201

Query: 267 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 326
           LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE
Sbjct: 202 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 261

Query: 327 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 386
           SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS
Sbjct: 262 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 321

Query: 387 KYHCMMKIQGLSPRTQQNL 406
           KYHCMMKIQGLSPRTQQNL
Sbjct: 322 KYHCMMKIQGLSPRTQQNL 340

BLAST of Moc02g05380 vs. NCBI nr
Match: XP_038901944.1 (APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901946.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901947.1 APO protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 585.1 bits (1507), Expect = 4.6e-163
Identity = 272/330 (82.42%), Postives = 302/330 (91.52%), Query Frame = 0

Query: 77  MTTMAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVA 136
           M TMAF+AL+RK  + VVQEF+LHRCYSSKV+LKKLRPMI KRI+NR K YPI  M PVA
Sbjct: 1   MKTMAFMALKRKFREIVVQEFVLHRCYSSKVNLKKLRPMILKRIQNREKNYPIRGMTPVA 60

Query: 137 QQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIG 196
           QQVLEAR +LIHGVSTLLK+FP++SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VHEWI 
Sbjct: 61  QQVLEARTMLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHLIRSCGGYKRGAKNQVHEWIR 120

Query: 197 GDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS 256
           GDLND++VPVE+FHLH MFQDVIKHDERFNFERVPAV ELCWQAGANPNDEN SSST NS
Sbjct: 121 GDLNDIIVPVESFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENQSSSTRNS 180

Query: 257 -DGGGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 316
            +GGGSGRD+ LS HE+M LA  T++AWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH
Sbjct: 181 GEGGGSGRDKPLSGHEMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 240

Query: 317 KARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVA 376
           KARLCGVFKYE WRGSHFWEKA++DDLVPP +VWHRRPQDPP+L+DEGRDYYGH PAV+A
Sbjct: 241 KARLCGVFKYEHWRGSHFWEKADIDDLVPPKLVWHRRPQDPPVLIDEGRDYYGHTPAVLA 300

Query: 377 LCTQAGVVAPSKYHCMMKIQGLSPRTQQNL 406
           LCTQAG +APSKYHCMMK+QG+SPR+Q  L
Sbjct: 301 LCTQAGALAPSKYHCMMKVQGISPRSQLKL 330

BLAST of Moc02g05380 vs. NCBI nr
Match: XP_023532078.1 (APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.9 bits (1483), Expect = 2.8e-160
Identity = 271/325 (83.38%), Postives = 295/325 (90.77%), Query Frame = 0

Query: 80  MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQV 139
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPI  MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 140 LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 199
           LEARA LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYK GAKNRVHEWI GDL
Sbjct: 61  LEARAKLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWIRGDL 120

Query: 200 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS--D 259
           ND+LVPVE+FHL  MFQDVIKHDERF+F+RVPAV ELCWQAGANPNDENLSSST NS  D
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFDRVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 260 GGGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKA 319
           G G+GRDE LSSHELM LAN T++AWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKA
Sbjct: 181 GNGNGRDEHLSSHELMVLANTTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKA 240

Query: 320 RLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALC 379
           RLCGVFKYESWRGSHFWEKA+VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC
Sbjct: 241 RLCGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALC 300

Query: 380 TQAGVVAPSKYHCMMKIQGLSPRTQ 403
            QAG +APSKYHCMMK+QGLS  +Q
Sbjct: 301 LQAGAIAPSKYHCMMKVQGLSHHSQ 325

BLAST of Moc02g05380 vs. NCBI nr
Match: XP_022971049.1 (APO protein 4, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 575.9 bits (1483), Expect = 2.8e-160
Identity = 271/323 (83.90%), Postives = 294/323 (91.02%), Query Frame = 0

Query: 80  MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQV 139
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPI  MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 140 LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 199
           LEARA+LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYKRGAKNRVHEWI GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 200 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGG 259
           ND+LVPVE+FHL  MFQDVIKHDERF+FERVPAV ELCWQAGANPNDENLSSST NSD  
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNSD-- 180

Query: 260 GSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARL 319
           G GRDE LSSHELM LAN T++AWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKARL
Sbjct: 181 GDGRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 320 CGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQ 379
           CGVFKYESWRGSHFWEK +VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCIQ 300

Query: 380 AGVVAPSKYHCMMKIQGLSPRTQ 403
           AG +APSKYHCMMK+QGLS  +Q
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQ 321

BLAST of Moc02g05380 vs. NCBI nr
Match: XP_004151879.1 (APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mitochondrial [Cucumis sativus] >XP_031737270.1 APO protein 4, mitochondrial [Cucumis sativus] >KGN63269.1 hypothetical protein Csa_022178 [Cucumis sativus])

HSP 1 Score: 571.6 bits (1472), Expect = 5.3e-159
Identity = 270/330 (81.82%), Postives = 298/330 (90.30%), Query Frame = 0

Query: 77  MTTMAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVA 136
           M TMAF+A+RRK   NVVQEFML RCYSSKV+LKKLRPMI KRI++RAK YPI  M PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 137 QQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIG 196
           QQVLEARA+LIHGVSTLLK+FP++SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VH+WI 
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 197 GDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS 256
           GDL D++VPVEAFHLH MFQDVIKHDERFNFERVPAV ELC QAGANP+D+NL+SST NS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 257 -DGGGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 316
            +GGGSG DE LS HE+M LA  T++AWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 317 KARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVA 376
           KARLCGVF YESWRGSHFWEKA+VDDLVPP IVWHRR QDPP+LVD+G+DYYGH PAVVA
Sbjct: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300

Query: 377 LCTQAGVVAPSKYHCMMKIQGLSPRTQQNL 406
           LCTQAGV+AP KYHCMMK+QGLSPR +  L
Sbjct: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of Moc02g05380 vs. ExPASy Swiss-Prot
Match: Q9LSZ0 (APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 7.3e-103
Identity = 174/292 (59.59%), Postives = 221/292 (75.68%), Query Frame = 0

Query: 108 DLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPE 167
           DL+KLRPMI KRIENRAK YP+  ++PVA+++L AR  LI  ++ LLK FP+++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 168 VYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNF 227
           V+VG++GHLI +C  Y R   NR+HEW+ G +ND+LVPVE++HLH + Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 228 ERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRD-EWLSSHELMPLANVTLKAWETL 287
           +RVPA+ ELC QAGA   +E L  S  + +   S  D   L + +L  +    L AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 288 RTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVPPN 347
           R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVFKYESWRG+H+WEKA V+DLVP  
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 348 IVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLS 399
           +VWHRRPQDP +LVDEGR YYGH PA+V+LC+  G + P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of Moc02g05380 vs. ExPASy Swiss-Prot
Match: Q8W4A5 (APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.4e-55
Identity = 110/297 (37.04%), Postives = 156/297 (52.53%), Query Frame = 0

Query: 129 INAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 188
           + +++P+A +V  AR  LI+ +  L+K   + +C +C E++VG  GH  +SC G     +
Sbjct: 130 VKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPNTSQR 189

Query: 189 NRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGAN----- 248
             +HEW    + DV+VP+EA+HL       I+HDERF+  RVPAV ELC Q G       
Sbjct: 190 KGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEIPEFP 249

Query: 249 -PNDENLSSSTGNSDGGGSGRDEW------------------------LSSHELMPLANV 308
                      G S+   +   E                          S  E + LA  
Sbjct: 250 AKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVSLAEE 309

Query: 309 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 368
           TL+AWE +R G +KL+ +Y  +VC YC EVHVGP+GHKA+ CG FK++   G H W+ A 
Sbjct: 310 TLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGWQSAV 369

Query: 369 VDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQ 396
           +DDL+PP  VWH    + P +  E R +YG  PAVV +C QAG V P  Y   M+++
Sbjct: 370 LDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRLE 426

BLAST of Moc02g05380 vs. ExPASy Swiss-Prot
Match: Q9XIR4 (APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 9.0e-53
Identity = 115/329 (34.95%), Postives = 167/329 (50.76%), Query Frame = 0

Query: 110 KKLRPM-ISKRIENRAKFYPINAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEV 169
           KKL  M I K+++       +  ++PVA QV++   +LI G++ LL   P+ +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 170 YVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFE 229
           +V   GH IR C G     +   H W+ G +NDVL+PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 230 RVPAVAELCWQAGAN---------------------------------PNDENLSSSTGN 289
           R+PA+ ELC QAG                                       +LSS    
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 290 SDG-GGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 349
            D  G   R    +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 350 HKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVV 404
           H  +LCG FK++   G H W+ A VD++ PPN VWH R      L    R +YG  PA+V
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of Moc02g05380 vs. ExPASy Swiss-Prot
Match: Q9FH50 (APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.6e-44
Identity = 97/300 (32.33%), Postives = 158/300 (52.67%), Query Frame = 0

Query: 132 MIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRV 191
           ++ VA  V   R +L+ G+S ++   P+  C+ C EV++G+QGH IR+C G   G+++  
Sbjct: 98  LVDVAHCVHRCRNMLLSGLSKIIHHVPVHRCRLCAEVHIGKQGHEIRTCTGPGSGSRSAT 157

Query: 192 HEWIGGDLNDVLVPVEAFHLH-RMFQDVIKHDERFNFERVPAVAELCWQAGAN----PND 251
           H W  G ++DV++  + FHL+ R  +  + HDERF   ++ AV ELC QAG +    P+ 
Sbjct: 158 HVWKRGRVSDVVLFPKCFHLYDRAVKPRVIHDERFTVPKISAVLELCIQAGVDLEKFPSK 217

Query: 252 ENLSS--------------STGNSDGGGSGRDEWLSSHE--------LMPLANVTLKAWE 311
                              + GNS+   +     +   +        L  L+  T+++W 
Sbjct: 218 RRSKPVYSIEGRIVDFEDVNDGNSELAVTSTTTLIQEDDRCKEEKKSLKELSFETMESWF 277

Query: 312 TLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVP 371
            +  GV+KL+  Y    C YC E+ VGP GHK R+C   K++   G H W++A +DD+V 
Sbjct: 278 EMVLGVRKLMERYRVWTCGYCPEIQVGPKGHKVRMCKATKHQMRDGMHAWQEATIDDVVG 337

Query: 372 PNIVWH-RRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLSPRTQQ 404
           P  VWH R P D  +L +  + +YG  PAV+ +C Q G   P +Y+ MM++  + P+  +
Sbjct: 338 PTYVWHVRDPTDGSVLDNSLKRFYGKAPAVIEMCVQGGAPVPDQYNSMMRLDVVYPQRDE 397

BLAST of Moc02g05380 vs. ExPASy TrEMBL
Match: A0A6J1CIR9 (APO protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011348 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.3e-189
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 87  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 146
           RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL
Sbjct: 22  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 81

Query: 147 IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 206
           IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV
Sbjct: 82  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 141

Query: 207 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 266
           EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW
Sbjct: 142 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 201

Query: 267 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 326
           LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE
Sbjct: 202 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 261

Query: 327 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 386
           SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS
Sbjct: 262 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 321

Query: 387 KYHCMMKIQGLSPRTQQNL 406
           KYHCMMKIQGLSPRTQQNL
Sbjct: 322 KYHCMMKIQGLSPRTQQNL 340

BLAST of Moc02g05380 vs. ExPASy TrEMBL
Match: A0A6J1I7G7 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.4e-160
Identity = 271/323 (83.90%), Postives = 294/323 (91.02%), Query Frame = 0

Query: 80  MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQV 139
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPI  MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 140 LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 199
           LEARA+LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYKRGAKNRVHEWI GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 200 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGG 259
           ND+LVPVE+FHL  MFQDVIKHDERF+FERVPAV ELCWQAGANPNDENLSSST NSD  
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNSD-- 180

Query: 260 GSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARL 319
           G GRDE LSSHELM LAN T++AWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKARL
Sbjct: 181 GDGRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 320 CGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQ 379
           CGVFKYESWRGSHFWEK +VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCIQ 300

Query: 380 AGVVAPSKYHCMMKIQGLSPRTQ 403
           AG +APSKYHCMMK+QGLS  +Q
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQ 321

BLAST of Moc02g05380 vs. ExPASy TrEMBL
Match: A0A0A0LQG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.6e-159
Identity = 270/330 (81.82%), Postives = 298/330 (90.30%), Query Frame = 0

Query: 77  MTTMAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVA 136
           M TMAF+A+RRK   NVVQEFML RCYSSKV+LKKLRPMI KRI++RAK YPI  M PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 137 QQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIG 196
           QQVLEARA+LIHGVSTLLK+FP++SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VH+WI 
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 197 GDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS 256
           GDL D++VPVEAFHLH MFQDVIKHDERFNFERVPAV ELC QAGANP+D+NL+SST NS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 257 -DGGGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 316
            +GGGSG DE LS HE+M LA  T++AWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 317 KARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVA 376
           KARLCGVF YESWRGSHFWEKA+VDDLVPP IVWHRR QDPP+LVD+G+DYYGH PAVVA
Sbjct: 241 KARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVA 300

Query: 377 LCTQAGVVAPSKYHCMMKIQGLSPRTQQNL 406
           LCTQAGV+AP KYHCMMK+QGLSPR +  L
Sbjct: 301 LCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of Moc02g05380 vs. ExPASy TrEMBL
Match: A0A6J1G8H9 (APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451698 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 4.4e-159
Identity = 267/323 (82.66%), Postives = 293/323 (90.71%), Query Frame = 0

Query: 80  MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQV 139
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPI  MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 140 LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 199
           LEARA+LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYK GAKNRVHEW  GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWFRGDL 120

Query: 200 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGG 259
           ND+LVPVE+FHLH MFQDVIKHDERF+FERVPAV ELCWQAGANPNDE+LSS T NSD  
Sbjct: 121 NDILVPVESFHLHHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDEHLSSGTWNSD-- 180

Query: 260 GSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARL 319
           G+GRDE LSSHELM LA+ T++AWET+RTGV KLLMVYP KVCK+CSEVHVGPSGHKARL
Sbjct: 181 GNGRDEHLSSHELMVLASTTIRAWETVRTGVHKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 320 CGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQ 379
           CGVFKYESWRGSHFWEKA+VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCLQ 300

Query: 380 AGVVAPSKYHCMMKIQGLSPRTQ 403
           AG +APSKYHCMMK+QGLS  +Q
Sbjct: 301 AGAIAPSKYHCMMKVQGLSHHSQ 321

BLAST of Moc02g05380 vs. ExPASy TrEMBL
Match: A0A5A7TJ18 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 8.2e-158
Identity = 266/325 (81.85%), Postives = 294/325 (90.46%), Query Frame = 0

Query: 77  MTTMAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVA 136
           M TMAF+A+RRK   NVVQEFML RCYSSKV+LKKLRPMI KRI++RAK YPI  M PVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 137 QQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIG 196
           QQVLEARA+LIHGVSTLLK+FP++SCK+CPEVYVG +GHLIRSCGGYKRGAKN+VH+WI 
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 197 GDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS 256
           GDLND++VPVEAFHLH MFQDVIKHDERFNFERVPAV ELC QAG NP+D++L+SST NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 257 -DGGGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGH 316
            +GGGSG DE  S HE+M LA  T++AWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGH
Sbjct: 181 AEGGGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGH 240

Query: 317 KARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVA 376
           KARLCGVFKYESWRGSHFWEKA+VDDLVPP IVWHRR QDPP+LVD+GRDYYGH PAVVA
Sbjct: 241 KARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVA 300

Query: 377 LCTQAGVVAPSKYHCMMKIQGLSPR 401
           LCTQAG +AP KYHCMMK+QGLSPR
Sbjct: 301 LCTQAGAIAPFKYHCMMKVQGLSPR 325

BLAST of Moc02g05380 vs. TAIR 10
Match: AT3G21740.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 375.6 bits (963), Expect = 5.2e-104
Identity = 174/292 (59.59%), Postives = 221/292 (75.68%), Query Frame = 0

Query: 108 DLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPE 167
           DL+KLRPMI KRIENRAK YP+  ++PVA+++L AR  LI  ++ LLK FP+++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 168 VYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNF 227
           V+VG++GHLI +C  Y R   NR+HEW+ G +ND+LVPVE++HLH + Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 228 ERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRD-EWLSSHELMPLANVTLKAWETL 287
           +RVPA+ ELC QAGA   +E L  S  + +   S  D   L + +L  +    L AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 288 RTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVPPN 347
           R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVFKYESWRG+H+WEKA V+DLVP  
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 348 IVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLS 399
           +VWHRRPQDP +LVDEGR YYGH PA+V+LC+  G + P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of Moc02g05380 vs. TAIR 10
Match: AT5G57930.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 216.1 bits (549), Expect = 5.3e-56
Identity = 110/297 (37.04%), Postives = 156/297 (52.53%), Query Frame = 0

Query: 129 INAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 188
           + +++P+A +V  AR  LI+ +  L+K   + +C +C E++VG  GH  +SC G     +
Sbjct: 130 VKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPNTSQR 189

Query: 189 NRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGAN----- 248
             +HEW    + DV+VP+EA+HL       I+HDERF+  RVPAV ELC Q G       
Sbjct: 190 KGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEIPEFP 249

Query: 249 -PNDENLSSSTGNSDGGGSGRDEW------------------------LSSHELMPLANV 308
                      G S+   +   E                          S  E + LA  
Sbjct: 250 AKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVSLAEE 309

Query: 309 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 368
           TL+AWE +R G +KL+ +Y  +VC YC EVHVGP+GHKA+ CG FK++   G H W+ A 
Sbjct: 310 TLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGWQSAV 369

Query: 369 VDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQ 396
           +DDL+PP  VWH    + P +  E R +YG  PAVV +C QAG V P  Y   M+++
Sbjct: 370 LDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRLE 426

BLAST of Moc02g05380 vs. TAIR 10
Match: AT5G57930.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 216.1 bits (549), Expect = 5.3e-56
Identity = 110/297 (37.04%), Postives = 156/297 (52.53%), Query Frame = 0

Query: 129 INAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 188
           + +++P+A +V  AR  LI+ +  L+K   + +C +C E++VG  GH  +SC G     +
Sbjct: 133 VKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPNTSQR 192

Query: 189 NRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGAN----- 248
             +HEW    + DV+VP+EA+HL       I+HDERF+  RVPAV ELC Q G       
Sbjct: 193 KGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEIPEFP 252

Query: 249 -PNDENLSSSTGNSDGGGSGRDEW------------------------LSSHELMPLANV 308
                      G S+   +   E                          S  E + LA  
Sbjct: 253 AKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVSLAEE 312

Query: 309 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 368
           TL+AWE +R G +KL+ +Y  +VC YC EVHVGP+GHKA+ CG FK++   G H W+ A 
Sbjct: 313 TLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGWQSAV 372

Query: 369 VDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQ 396
           +DDL+PP  VWH    + P +  E R +YG  PAVV +C QAG V P  Y   M+++
Sbjct: 373 LDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRLE 429

BLAST of Moc02g05380 vs. TAIR 10
Match: AT1G64810.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 209.1 bits (531), Expect = 6.4e-54
Identity = 115/329 (34.95%), Postives = 167/329 (50.76%), Query Frame = 0

Query: 110 KKLRPM-ISKRIENRAKFYPINAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEV 169
           KKL  M I K+++       +  ++PVA QV++   +LI G++ LL   P+ +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 170 YVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFE 229
           +V   GH IR C G     +   H W+ G +NDVL+PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 230 RVPAVAELCWQAGAN---------------------------------PNDENLSSSTGN 289
           R+PA+ ELC QAG                                       +LSS    
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 290 SDG-GGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 349
            D  G   R    +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 350 HKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVV 404
           H  +LCG FK++   G H W+ A VD++ PPN VWH R      L    R +YG  PA+V
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of Moc02g05380 vs. TAIR 10
Match: AT1G64810.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 209.1 bits (531), Expect = 6.4e-54
Identity = 115/329 (34.95%), Postives = 167/329 (50.76%), Query Frame = 0

Query: 110 KKLRPM-ISKRIENRAKFYPINAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEV 169
           KKL  M I K+++       +  ++PVA QV++   +LI G++ LL   P+ +C  C  V
Sbjct: 127 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 186

Query: 170 YVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFE 229
           +V   GH IR C G     +   H W+ G +NDVL+PVE++H++  F   IKH+ RF +E
Sbjct: 187 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 246

Query: 230 RVPAVAELCWQAGAN---------------------------------PNDENLSSSTGN 289
           R+PA+ ELC QAG                                       +LSS    
Sbjct: 247 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 306

Query: 290 SDG-GGSGRDEWLSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 349
            D  G   R    +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 307 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 366

Query: 350 HKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVV 404
           H  +LCG FK++   G H W+ A VD++ PPN VWH R      L    R +YG  PA+V
Sbjct: 367 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140768.11.1e-188100.00APO protein 4, mitochondrial [Momordica charantia][more]
XP_038901944.14.6e-16382.42APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, ... [more]
XP_023532078.12.8e-16083.38APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022971049.12.8e-16083.90APO protein 4, mitochondrial-like [Cucurbita maxima][more]
XP_004151879.15.3e-15981.82APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mi... [more]
Match NameE-valueIdentityDescription
Q9LSZ07.3e-10359.59APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2[more]
Q8W4A57.4e-5537.04APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1[more]
Q9XIR49.0e-5334.95APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1[more]
Q9FH502.6e-4432.33APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CIR95.3e-189100.00APO protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011348 PE=4... [more]
A0A6J1I7G71.4e-16083.90APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE... [more]
A0A0A0LQG52.6e-15981.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1[more]
A0A6J1G8H94.4e-15982.66APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451698 ... [more]
A0A5A7TJ188.2e-15881.85APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180... [more]
Match NameE-valueIdentityDescription
AT3G21740.15.2e-10459.59Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.15.3e-5637.04Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.25.3e-5637.04Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.16.4e-5434.95Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.26.4e-5434.95Arabidopsis thaliana protein of unknown function (DUF794) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023342APO domainPFAMPF05634APO_RNA-bindcoord: 270..385
e-value: 8.3E-23
score: 80.8
coord: 105..243
e-value: 1.2E-39
score: 135.8
IPR023342APO domainPROSITEPS51499APOcoord: 161..246
score: 21.005489
IPR023342APO domainPROSITEPS51499APOcoord: 301..386
score: 21.735817
NoneNo IPR availablePANTHERPTHR10388EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1coord: 88..400
NoneNo IPR availablePANTHERPTHR10388:SF20APO PROTEIN 4, MITOCHONDRIALcoord: 88..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g05380.1Moc02g05380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding