Moc02g04990 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g04990
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ervatamin-B
Location	chr2: 3676701 .. 3677883 (-)
RNA-Seq Expression	Moc02g04990
Synteny	Moc02g04990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCATATGGAATGATTCGAAATGTGGGTTTTATGTGGCTGATTCTCTGTGTTTTTTGGACACTTTCAATGGCATCCGTTGCAGAGGACAACCCTCCCGGAGATGGTTCCGATGACATGAGAGACAGGTACCAGAAATGGATCGATAAATATGGTCGAGAATACAAGAGTGGAGAGGAGCGGGAGAAGAGGTTTCCGATTTATCAGTCTAATGTTCAATACATTGACTACTTCAATTCTCTGAATCGCTCATATACTCTGGCTGACAACATGTTTGCAGACCTTACAAATGATGAGTTTAAGACAACTTATTTGGGATATCTAACTGATTGGTCTCCTGACACATGCTTCAAATATGGCAACATTGTTAATTTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTAACTCCGATAAAGGACCAAGGCCAATGCGGTATGAACTCATCCAACTTATAAGAAATCACCATTAACATGTTCTCTTTTCTTCTGATTTTGAGTTCTTTGGATAACAAGTAGCCCTATATCCACTATAAGTCATCTCTTAGTAGATGAACATACTTACCAGTTTTGAAGAATTGACACAGGGAGTTGCTGGGCGTTCTCGGCGGTAGCAGCTGTGGAAGGCATCACCAAAATAAAAACAGGAAAGTTGGTGTCTCTATCAGAACAAGAGCTCCTGGACTGCGATGTTATCTCGGGGAACCAGGGATGTAGTGGTGGTTTCATGCCCAAAGCATTTGAGTTCATCAAGAAAATTGGAATCACTACAGAAAAAGAATATCCATACAGGGGAGTTGAAAATGTATGCAACAAACAAAAAGTGAGATACCACTCTGCGACAATAAGTGGGTATGAAAAAGTACCTGCCAATGATGAGAAAAGCCTAAAAGCTGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGGGGGGTATGATTTTCAGTTCTATTCTGGCGGAATCTTCTCAGGGAATTGTGGAAAGCAACTCAATCATGGAGTGACAATAGTTGGGTATGGGGAAGACGTCGGTAAAAGTTACTGGCTTGTCAAGAATTCATGGGGTACTAGCTGGGGTGAATATGGTTATGTAAGAATGAAAAGTAATTCAAGTGATAAGCGAGGTACTTGTGGCATAGCCATGGATGCTAGCTACCCCATCAAAGACTGA

mRNA sequence

ATGGAAGCATATGGAATGATTCGAAATGTGGGTTTTATGTGGCTGATTCTCTGTGTTTTTTGGACACTTTCAATGGCATCCGTTGCAGAGGACAACCCTCCCGGAGATGGTTCCGATGACATGAGAGACAGGTACCAGAAATGGATCGATAAATATGGTCGAGAATACAAGAGTGGAGAGGAGCGGGAGAAGAGGTTTCCGATTTATCAGTCTAATGTTCAATACATTGACTACTTCAATTCTCTGAATCGCTCATATACTCTGGCTGACAACATGTTTGCAGACCTTACAAATGATGAGTTTAAGACAACTTATTTGGGATATCTAACTGATTGGTCTCCTGACACATGCTTCAAATATGGCAACATTGTTAATTTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTAACTCCGATAAAGGACCAAGGCCAATGCGGGAGTTGCTGGGCGTTCTCGGCGGTAGCAGCTGTGGAAGGCATCACCAAAATAAAAACAGGAAAGTTGGTGTCTCTATCAGAACAAGAGCTCCTGGACTGCGATGTTATCTCGGGGAACCAGGGATGTAGTGGTGGTTTCATGCCCAAAGCATTTGAGTTCATCAAGAAAATTGGAATCACTACAGAAAAAGAATATCCATACAGGGGAGTTGAAAATGTATGCAACAAACAAAAAGTGAGATACCACTCTGCGACAATAAGTGGGTATGAAAAAGTACCTGCCAATGATGAGAAAAGCCTAAAAGCTGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGGGGGGTATGATTTTCAGTTCTATTCTGGCGGAATCTTCTCAGGGAATTGTGGAAAGCAACTCAATCATGGAGTGACAATAGTTGGGTATGGGGAAGACGTCGGTAAAAGTTACTGGCTTGTCAAGAATTCATGGGGTACTAGCTGGGGTGAATATGGTTATGTAAGAATGAAAAGTAATTCAAGTGATAAGCGAGGTACTTGTGGCATAGCCATGGATGCTAGCTACCCCATCAAAGACTGA

Coding sequence (CDS)

ATGGAAGCATATGGAATGATTCGAAATGTGGGTTTTATGTGGCTGATTCTCTGTGTTTTTTGGACACTTTCAATGGCATCCGTTGCAGAGGACAACCCTCCCGGAGATGGTTCCGATGACATGAGAGACAGGTACCAGAAATGGATCGATAAATATGGTCGAGAATACAAGAGTGGAGAGGAGCGGGAGAAGAGGTTTCCGATTTATCAGTCTAATGTTCAATACATTGACTACTTCAATTCTCTGAATCGCTCATATACTCTGGCTGACAACATGTTTGCAGACCTTACAAATGATGAGTTTAAGACAACTTATTTGGGATATCTAACTGATTGGTCTCCTGACACATGCTTCAAATATGGCAACATTGTTAATTTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTAACTCCGATAAAGGACCAAGGCCAATGCGGGAGTTGCTGGGCGTTCTCGGCGGTAGCAGCTGTGGAAGGCATCACCAAAATAAAAACAGGAAAGTTGGTGTCTCTATCAGAACAAGAGCTCCTGGACTGCGATGTTATCTCGGGGAACCAGGGATGTAGTGGTGGTTTCATGCCCAAAGCATTTGAGTTCATCAAGAAAATTGGAATCACTACAGAAAAAGAATATCCATACAGGGGAGTTGAAAATGTATGCAACAAACAAAAAGTGAGATACCACTCTGCGACAATAAGTGGGTATGAAAAAGTACCTGCCAATGATGAGAAAAGCCTAAAAGCTGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGGGGGGTATGATTTTCAGTTCTATTCTGGCGGAATCTTCTCAGGGAATTGTGGAAAGCAACTCAATCATGGAGTGACAATAGTTGGGTATGGGGAAGACGTCGGTAAAAGTTACTGGCTTGTCAAGAATTCATGGGGTACTAGCTGGGGTGAATATGGTTATGTAAGAATGAAAAGTAATTCAAGTGATAAGCGAGGTACTTGTGGCATAGCCATGGATGCTAGCTACCCCATCAAAGACTGA

Protein sequence

MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKYGNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD

Homology

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BLAST of Moc02g04990 vs. NCBI nr
Match: XP_022140756.1 (ervatamin-B [Momordica charantia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.6e-204
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60
           MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE
Sbjct: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60

Query: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120
           EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY
Sbjct: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120

Query: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180
           GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD
Sbjct: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180

Query: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240
           CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV
Sbjct: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240

Query: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300
           PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS
Sbjct: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300

Query: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD
Sbjct: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 343

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BLAST of Moc02g04990 vs. NCBI nr
Match: XP_038902939.1 (ervatamin-B [Benincasa hispida])

HSP 1 Score: 575.5 bits (1482), Expect = 3.1e-160
Identity = 273/343 (79.59%), Postives = 300/343 (87.46%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60
           MEAY MI NVG M LIL V WT +M  +A D PPG  S D++ RYQKW+ KYGR+YKS E
Sbjct: 1   MEAYRMIWNVGLMSLILWVIWTPTMVFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSRE 60

Query: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120
           E E+RF IYQ NVQYID FNSL+ SYTLA+N FADLTNDEFK TYLGY TDW PDTCF+Y
Sbjct: 61  EWERRFTIYQLNVQYIDNFNSLDHSYTLAENNFADLTNDEFKETYLGYKTDWLPDTCFRY 120

Query: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180
           GN+V+LPTNV+WRKEGAVTPIK+QGQCGSCWAFSAVAAVEGI KIKTGKL+SLSEQEL+D
Sbjct: 121 GNMVDLPTNVNWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 180

Query: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240
           CDV SGNQGC+GGFM KAF+FIKK G+TTE EYPYRG+E+ CNKQKVR H+  ISGYEKV
Sbjct: 181 CDVASGNQGCNGGFMDKAFQFIKKTGLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKV 240

Query: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300
           PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGG+FSGNCGKQLNHGV IVGYG+   KS
Sbjct: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYGQASNKS 300

Query: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           YWLVKNSWGT WGE GY+RMK +S+DKRGTCGIAM ASYPIKD
Sbjct: 301 YWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 343

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BLAST of Moc02g04990 vs. NCBI nr
Match: XP_038902648.1 (ervatamin-B-like [Benincasa hispida])

HSP 1 Score: 557.8 bits (1436), Expect = 6.7e-155
Identity = 266/344 (77.33%), Postives = 297/344 (86.34%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60
           MEAY MI NVG M LIL V WT +M  +A D PPG  S D++ RYQKW+ KYGR+YKS E
Sbjct: 1   MEAYRMIWNVGLMSLILWVIWTPTMEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSRE 60

Query: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120
           E E+RF IYQ NVQYID FNSL+ SYTLA+N   DLTNDEFK TYLGY TDW PDTCF+Y
Sbjct: 61  EWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRY 120

Query: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180
           GN+V+LPTNV+WRKEGAVTPI +QGQCG+CWAFSAVAAVEGI KIKTGKL+SLSEQEL+D
Sbjct: 121 GNMVHLPTNVNWRKEGAVTPINNQGQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 180

Query: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240
           CDV SGNQGC+GGFM KAF+FIKK  +TTE EYPYRG+E+ CNKQKVR H+  ISGYEKV
Sbjct: 181 CDVASGNQGCNGGFMDKAFQFIKKTRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKV 240

Query: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGE-DVGK 300
           PANDEKSLKA VANQPVS+AIDAGGYDFQFYSGG+FSGNCGKQLNHGV IVGY +  + K
Sbjct: 241 PANDEKSLKAVVANQPVSLAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQATLHK 300

Query: 301 SYWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           SYWLVKNSWGT+WGE GY+RMKS+S+DKRGTCGIAM ASYPIKD
Sbjct: 301 SYWLVKNSWGTNWGESGYIRMKSDSTDKRGTCGIAMMASYPIKD 344

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BLAST of Moc02g04990 vs. NCBI nr
Match: AKO60151.1 (cysteine proteinase 1, partial [Citrullus lanatus])

HSP 1 Score: 552.4 bits (1422), Expect = 2.8e-153
Identity = 261/319 (81.82%), Postives = 284/319 (89.03%), Query Frame = 0


Query: 25  MASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNR 84
           MAS+  D  PG  S D++DRYQKW+ KYGREYKS EE E+RF IYQ NVQYID FNSLN 
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 85  SYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKYGNIVNLPTNVDWRKEGAVTPIKDQ 144
           SYTLA+N FADLTNDEFKTTYLG+ TDW PDT F+YGN+VNLPTNVDWRKE AVTP+KDQ
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 145 GQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFEFIKK 204
           GQCGSCWAFSAVAAVEGI KIKTGKL+SLSEQEL+DCDV SGNQGC+GG+M KAFEFIKK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 205 IGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSVAIDAG 264
            G+TTE EYPYRG+E+VCNKQKVRY + TISGYEKVP NDEKSLKAAVANQPVSVAIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 265 GYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVKNSWGTSWGEYGYVRMKSNS 324
           GYDFQFYSGG+FSGNCGKQLNHGV IVGYGE   K+YWLVKNSWGT WGE GY+RMK +S
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDS 300

Query: 325 SDKRGTCGIAMDASYPIKD 344
           +DKRGTCGIAM ASYPIKD
Sbjct: 301 TDKRGTCGIAMMASYPIKD 319

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BLAST of Moc02g04990 vs. NCBI nr
Match: XP_023513224.1 (ervatamin-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531.2 bits (1367), Expect = 6.7e-147
Identity = 257/343 (74.93%), Postives = 290/343 (84.55%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60
           MEAY  I N+G   LIL V  T SMAS+A D+P    S+ ++DRY+KW++K+ REYKS E
Sbjct: 1   MEAYKTIWNMGLTSLILWVVCTPSMASMATDSP----SNGLQDRYKKWMNKHSREYKSRE 60

Query: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120
           E+E+RF +YQ NVQYID FNSLN SYTLA+N FADLTNDEFKTTYLGY T   PDTCF+Y
Sbjct: 61  EQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKTTYLGYQTHCLPDTCFRY 120

Query: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180
            ++ +LPT+VDWR E AVTPIKDQGQCGSCWAFSAVAAVEGI KI+TGKL SLSEQEL+D
Sbjct: 121 EHVNSLPTHVDWRMEDAVTPIKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVD 180

Query: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240
           CD+ISGNQGC GGFM KAFE+IK+ G+TTE+EYPYRG+E  CN QKVRYHS TISGYEKV
Sbjct: 181 CDIISGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKV 240

Query: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300
           P N+EK LKAAVANQPVSVAIDAGGYDFQFYS GIFSG+CGKQLNHGV IVGYGE    +
Sbjct: 241 PMNNEKKLKAAVANQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT 300

Query: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           YWLVKNSWGT WGE GY+RMK +S DKRG CGIAM+ASYPIKD
Sbjct: 301 YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 339

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BLAST of Moc02g04990 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 343.6 bits (880), Expect = 2.6e-93
Identity = 176/342 (51.46%), Postives = 231/342 (67.54%), Query Frame = 0


Query: 13  MWLILCVFWTLSMASVAEDNPPGDGSDD-MRDRYQKWIDKYGREYKSGEEREKRFPIYQS 72
           +W++L     L +A+  + +     S++ + D Y++W   +      G E+ KRF ++++
Sbjct: 7   LWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLG-EKHKRFNVFKA 66

Query: 73  NVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDW--------SPDTCFKYGNI 132
           N+ ++   N +++ Y L  N FAD+TN EF++TY G   +           +  F Y  +
Sbjct: 67  NLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYEKV 126

Query: 133 VNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDV 192
           V++P +VDWRK+GAVT +KDQGQCGSCWAFS V AVEGI +IKT KLV+LSEQEL+DCD 
Sbjct: 127 VSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCDK 186

Query: 193 ISGNQGCSGGFMPKAFEFIK-KIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPA 252
              NQGC+GG M  AFEFIK K GITTE  YPY+  E  C+  KV   + +I G+E VPA
Sbjct: 187 -EENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPA 246

Query: 253 NDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV-GKSY 312
           NDE +L  AVANQPVSVAIDAGG DFQFYS G+F+G+C   LNHGV IVGYG  V G +Y
Sbjct: 247 NDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTVDGTNY 306

Query: 313 WLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           W+V+NSWG  WGE+GY+RM+ N S K G CGIAM  SYPIK+
Sbjct: 307 WIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIKN 346

[Close]

BLAST of Moc02g04990 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.4e-93
Identity = 175/342 (51.17%), Postives = 228/342 (66.67%), Query Frame = 0


Query: 13  MWLILCVFWTLSMASVAEDNPPG-DGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQS 72
           +W++L +   L +A+  + +    +  + + D Y++W   +      G E+ KRF ++++
Sbjct: 7   LWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLG-EKHKRFNVFKA 66

Query: 73  NVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLG--------YLTDWSPDTCFKYGNI 132
           NV ++   N +++ Y L  N FAD+TN EF++TY G        +         F Y  +
Sbjct: 67  NVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMYEKV 126

Query: 133 VNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDV 192
            ++P +VDWRK+GAVT +KDQGQCGSCWAFS + AVEGI +IKT KLVSLSEQEL+DCD 
Sbjct: 127 GSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVDCDK 186

Query: 193 ISGNQGCSGGFMPKAFEFIK-KIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPA 252
              NQGC+GG M  AFEFIK K GITTE  YPY   E  C++ KV   + +I G+E VP 
Sbjct: 187 -EENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHENVPV 246

Query: 253 NDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV-GKSY 312
           NDE +L  AVANQPVSVAIDAGG DFQFYS G+F+G+C   LNHGV IVGYG  V G +Y
Sbjct: 247 NDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTVDGTNY 306

Query: 313 WLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           W+V+NSWG  WGE GY+RM+ N S K G CGIAM ASYPIK+
Sbjct: 307 WIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKN 346

[Close]

BLAST of Moc02g04990 vs. ExPASy Swiss-Prot
Match: A2XQE8 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_14861 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 6.4e-92
Identity = 170/312 (54.49%), Postives = 222/312 (71.15%), Query Frame = 0


Query: 38  SDD--MRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFAD 97
           SDD  M  R+++W+ +YGR Y+   E+ +RF ++++NV +I+ FN+ N ++ L  N FAD
Sbjct: 28  SDDAAMAARHERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFAD 87

Query: 98  LTNDEFK--TTYLGYLTDWS-PDTCFKYG--NIVNLPTNVDWRKEGAVTPIKDQGQCGSC 157
           LTNDEF+   T  G++   +   T F+Y   NI  LP  VDWR +GAVTPIKDQGQCG C
Sbjct: 88  LTNDEFRWTKTNKGFIPSTTRVPTGFRYENVNIDALPATVDWRTKGAVTPIKDQGQCGCC 147

Query: 158 WAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFEF-IKKIGITT 217
           WAFSAVAA+EGI K+ TGKL+SLSEQEL+DCDV   +QGC GG M  AF+F IK  G+TT
Sbjct: 148 WAFSAVAAMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLTT 207

Query: 218 EKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSVAIDAGGYDFQ 277
           E  YPY   ++ C  + V    A+I GYE VPAN+E +L  AVANQPVSVA+D G   FQ
Sbjct: 208 ESNYPYAAADDKC--KSVSNSVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDMTFQ 267

Query: 278 FYSGGIFSGNCGKQLNHGVTIVGYGE-DVGKSYWLVKNSWGTSWGEYGYVRMKSNSSDKR 337
           FY GG+ +G+CG  L+HG+  +GYG+   G  YWL+KNSWGT+WGE G++RM+ + SDKR
Sbjct: 268 FYKGGVMTGSCGTDLDHGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKDISDKR 327

Query: 338 GTCGIAMDASYP 341
           G CG+AM+ SYP
Sbjct: 328 GMCGLAMEPSYP 337

[Close]

BLAST of Moc02g04990 vs. ExPASy Swiss-Prot
Match: Q7XWK5 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. japonica OX=39947 GN=SAG39 PE=2 SV=2)

HSP 1 Score: 339.0 bits (868), Expect = 6.4e-92
Identity = 170/312 (54.49%), Postives = 222/312 (71.15%), Query Frame = 0


Query: 38  SDD--MRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFAD 97
           SDD  M  R+++W+ +YGR Y+   E+ +RF ++++NV +I+ FN+ N ++ L  N FAD
Sbjct: 28  SDDAAMAARHERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFAD 87

Query: 98  LTNDEFK--TTYLGYLTDWS-PDTCFKYG--NIVNLPTNVDWRKEGAVTPIKDQGQCGSC 157
           LTNDEF+   T  G++   +   T F+Y   NI  LP  VDWR +GAVTPIKDQGQCG C
Sbjct: 88  LTNDEFRWMKTNKGFIPSTTRVPTGFRYENVNIDALPATVDWRTKGAVTPIKDQGQCGCC 147

Query: 158 WAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFEF-IKKIGITT 217
           WAFSAVAA+EGI K+ TGKL+SLSEQEL+DCDV   +QGC GG M  AF+F IK  G+TT
Sbjct: 148 WAFSAVAAMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLTT 207

Query: 218 EKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSVAIDAGGYDFQ 277
           E  YPY   ++ C  + V    A+I GYE VPAN+E +L  AVANQPVSVA+D G   FQ
Sbjct: 208 ESNYPYAAADDKC--KSVSNSVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDMTFQ 267

Query: 278 FYSGGIFSGNCGKQLNHGVTIVGYGE-DVGKSYWLVKNSWGTSWGEYGYVRMKSNSSDKR 337
           FY GG+ +G+CG  L+HG+  +GYG+   G  YWL+KNSWGT+WGE G++RM+ + SDKR
Sbjct: 268 FYKGGVMTGSCGTDLDHGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKDISDKR 327

Query: 338 GTCGIAMDASYP 341
           G CG+AM+ SYP
Sbjct: 328 GMCGLAMEPSYP 337

[Close]

BLAST of Moc02g04990 vs. ExPASy Swiss-Prot
Match: Q9FJ47 (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=SAG12 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-91
Identity = 167/323 (51.70%), Postives = 224/323 (69.35%), Query Frame = 0


Query: 34  PGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSL--NRSYTLADN 93
           P D    M+ R+ +W+ K+GR Y   +E   R+ ++++NV+ I++ NS+   R++ LA N
Sbjct: 27  PLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVN 86

Query: 94  MFADLTNDEFKTTYLGY----------LTDWSPDTCFKYGNIVN--LPTNVDWRKEGAVT 153
            FADLTNDEF++ Y G+           T  SP   F+Y N+ +  LP +VDWRK+GAVT
Sbjct: 87  QFADLTNDEFRSMYTGFKGVSALSSQSQTKMSP---FRYQNVSSGALPVSVDWRKKGAVT 146

Query: 154 PIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAF 213
           PIK+QG CG CWAFSAVAA+EG T+IK GKL+SLSEQ+L+DCD  + + GC GG M  AF
Sbjct: 147 PIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAF 206

Query: 214 EFIKKI-GITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVS 273
           E IK   G+TTE  YPY+G +  CN +K    + +I+GYE VP NDE++L  AVA+QPVS
Sbjct: 207 EHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVS 266

Query: 274 VAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV-GKSYWLVKNSWGTSWGEYGY 333
           V I+ GG+DFQFYS G+F+G C   L+H VT +GYGE   G  YW++KNSWGT WGE GY
Sbjct: 267 VGIEGGGFDFQFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGY 326

Query: 334 VRMKSNSSDKRGTCGIAMDASYP 341
           +R++ +  DK+G CG+AM ASYP
Sbjct: 327 MRIQKDVKDKQGLCGLAMKASYP 344

[Close]

BLAST of Moc02g04990 vs. ExPASy TrEMBL
Match: A0A6J1CH04 (ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.2e-204
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60
           MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE
Sbjct: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60

Query: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120
           EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY
Sbjct: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120

Query: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180
           GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD
Sbjct: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180

Query: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240
           CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV
Sbjct: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240

Query: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300
           PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS
Sbjct: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300

Query: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD
Sbjct: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 343

[Close]

BLAST of Moc02g04990 vs. ExPASy TrEMBL
Match: A0A384S0D9 (Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.4e-153
Identity = 261/319 (81.82%), Postives = 284/319 (89.03%), Query Frame = 0


Query: 25  MASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNR 84
           MAS+  D  PG  S D++DRYQKW+ KYGREYKS EE E+RF IYQ NVQYID FNSLN 
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 85  SYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKYGNIVNLPTNVDWRKEGAVTPIKDQ 144
           SYTLA+N FADLTNDEFKTTYLG+ TDW PDT F+YGN+VNLPTNVDWRKE AVTP+KDQ
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 145 GQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFEFIKK 204
           GQCGSCWAFSAVAAVEGI KIKTGKL+SLSEQEL+DCDV SGNQGC+GG+M KAFEFIKK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 205 IGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSVAIDAG 264
            G+TTE EYPYRG+E+VCNKQKVRY + TISGYEKVP NDEKSLKAAVANQPVSVAIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 265 GYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVKNSWGTSWGEYGYVRMKSNS 324
           GYDFQFYSGG+FSGNCGKQLNHGV IVGYGE   K+YWLVKNSWGT WGE GY+RMK +S
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDS 300

Query: 325 SDKRGTCGIAMDASYPIKD 344
           +DKRGTCGIAM ASYPIKD
Sbjct: 301 TDKRGTCGIAMMASYPIKD 319

[Close]

BLAST of Moc02g04990 vs. ExPASy TrEMBL
Match: A0A6J1FYZ3 (ervatamin-B-like OS=Cucurbita moschata OX=3662 GN=LOC111449119 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 9.5e-147
Identity = 253/343 (73.76%), Postives = 290/343 (84.55%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60
           MEAY  I N+G   LIL +  T SMAS+A D+P    S+ ++DRY+KW++K+ REYKS E
Sbjct: 1   MEAYKTIWNMGLTSLILWIVCTPSMASMATDSP----SNGLQDRYKKWMNKHSREYKSRE 60

Query: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120
           E+E+RF +YQ NVQYID FNSLN SYTLA+N FADLTNDEFKTTYLGY T   PDTCF+Y
Sbjct: 61  EQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKTTYLGYQTHCLPDTCFRY 120

Query: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180
            ++++LPT+VDWR E AVTP+KDQGQCGSCWAFSAVAAVEGI KI+TGKL SLSEQEL+D
Sbjct: 121 DHVISLPTHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVD 180

Query: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240
           CD+ISGNQGC GGFM KAFE+IK+ G+TTE+EYPYRG+E  CN QKVRYHS TISGYEKV
Sbjct: 181 CDIISGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKV 240

Query: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300
           P N+EK LKAAVA+QPVSVAIDAGGYDFQFYS GIFSG+CGKQLNHGV IVGYGE    +
Sbjct: 241 PTNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT 300

Query: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           YWLVKNSWGT WGE GY+RMK +S DKRG CGIAM+ASYP KD
Sbjct: 301 YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPTKD 339

[Close]

BLAST of Moc02g04990 vs. ExPASy TrEMBL
Match: A0A5A7SQK0 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001250 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.6e-146
Identity = 258/346 (74.57%), Postives = 290/346 (83.82%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPG-DGSDDMRDRYQKWIDKYGREYKSG 60
           MEAY MI +V  + LIL VFWT +  S+A D P G   S +++DRYQKW+DKYGR+YKS 
Sbjct: 1   MEAYKMIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSR 60

Query: 61  EEREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSP--DTC 120
           EE E+RF IYQ+NVQYID FNSLN SYTLA+N F DLTN+EF  TYLGY T   P  DT 
Sbjct: 61  EEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTF 120

Query: 121 FKYGNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQE 180
           F+YGN+VNLPTNVDWRKEGAVTPIK+QGQCGSCWAFSAVAAVEGI KIK GKL+SLSEQE
Sbjct: 121 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQE 180

Query: 181 LLDCDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGY 240
           L+DCDV SGNQGC+GG+M KAFEFIKK G+TTE EYPY    + C+KQK +Y S +ISGY
Sbjct: 181 LVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGY 240

Query: 241 EKVPANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV 300
           EKVP NDEKSL+AAVA QPVSVAIDAGG DFQFYSGGIFSGNCGKQLNHGV IVGYGED 
Sbjct: 241 EKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDS 300

Query: 301 GKSYWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
            ++YWLVKNSWGTSWGE GY+RM  +S+DK+GTCGIAM ASYPIKD
Sbjct: 301 NQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

[Close]

BLAST of Moc02g04990 vs. ExPASy TrEMBL
Match: A0A1S3C828 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.6e-146
Identity = 258/346 (74.57%), Postives = 290/346 (83.82%), Query Frame = 0


Query: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPG-DGSDDMRDRYQKWIDKYGREYKSG 60
           MEAY MI +V  + LIL VFWT +  S+A D P G   S +++DRYQKW+DKYGR+YKS 
Sbjct: 1   MEAYKMIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSR 60

Query: 61  EEREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSP--DTC 120
           EE E+RF IYQ+NVQYID FNSLN SYTLA+N F DLTN+EF  TYLGY T   P  DT 
Sbjct: 61  EEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTF 120

Query: 121 FKYGNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQE 180
           F+YGN+VNLPTNVDWRKEGAVTPIK+QGQCGSCWAFSAVAAVEGI KIK GKL+SLSEQE
Sbjct: 121 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQE 180

Query: 181 LLDCDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGY 240
           L+DCDV SGNQGC+GG+M KAFEFIKK G+TTE EYPY    + C+KQK +Y S +ISGY
Sbjct: 181 LVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGY 240

Query: 241 EKVPANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV 300
           EKVP NDEKSL+AAVA QPVSVAIDAGG DFQFYSGGIFSGNCGKQLNHGV IVGYGED 
Sbjct: 241 EKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDS 300

Query: 301 GKSYWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
            ++YWLVKNSWGTSWGE GY+RM  +S+DK+GTCGIAM ASYPIKD
Sbjct: 301 NQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

[Close]

BLAST of Moc02g04990 vs. TAIR 10
Match: AT1G06260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.5e-96
Identity = 177/344 (51.45%), Postives = 228/344 (66.28%), Query Frame = 0


Query: 6   MIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKR 65
           ++RN      +L  F  ++    + D+   D    ++ R++KW+  + + Y   +E   R
Sbjct: 4   VLRNSNLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLR 63

Query: 66  FPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTD------WSPDTCFK 125
           F IYQSNVQ IDY NSL+  + L DN FAD+TN EFK  +LG  T            C  
Sbjct: 64  FGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDP 123

Query: 126 YGNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELL 185
            GN+   P  VDWR +GAVTPI++QG+CG CWAFSAVAA+EGI KIKTG LVSLSEQ+L+
Sbjct: 124 AGNV---PDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLI 183

Query: 186 DCDVISGNQGCSGGFMPKAFEFIK-KIGITTEKEYPYRGVENVCNKQKVRYHSATISGYE 245
           DCDV + N+GCSGG M  AFEFIK   G+ TE +YPY G+E  C+++K +    TI GY+
Sbjct: 184 DCDVGTYNKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTCDQEKSKNKVVTIQGYQ 243

Query: 246 KVPANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVG 305
           KV A +E SL+ A A QPVSV IDAGG+ FQ YS G+F+  CG  LNHGVT+VGYG +  
Sbjct: 244 KV-AQNEASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGD 303

Query: 306 KSYWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIK 343
           + YW+VKNSWGT WGE GY+RM+   S+  G CGIAM ASYP++
Sbjct: 304 QKYWIVKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMASYPLQ 343

[Close]

BLAST of Moc02g04990 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 337.4 bits (864), Expect = 1.3e-92
Identity = 167/323 (51.70%), Postives = 224/323 (69.35%), Query Frame = 0


Query: 34  PGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSL--NRSYTLADN 93
           P D    M+ R+ +W+ K+GR Y   +E   R+ ++++NV+ I++ NS+   R++ LA N
Sbjct: 27  PLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVN 86

Query: 94  MFADLTNDEFKTTYLGY----------LTDWSPDTCFKYGNIVN--LPTNVDWRKEGAVT 153
            FADLTNDEF++ Y G+           T  SP   F+Y N+ +  LP +VDWRK+GAVT
Sbjct: 87  QFADLTNDEFRSMYTGFKGVSALSSQSQTKMSP---FRYQNVSSGALPVSVDWRKKGAVT 146

Query: 154 PIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAF 213
           PIK+QG CG CWAFSAVAA+EG T+IK GKL+SLSEQ+L+DCD  + + GC GG M  AF
Sbjct: 147 PIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAF 206

Query: 214 EFIKKI-GITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVS 273
           E IK   G+TTE  YPY+G +  CN +K    + +I+GYE VP NDE++L  AVA+QPVS
Sbjct: 207 EHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVS 266

Query: 274 VAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV-GKSYWLVKNSWGTSWGEYGY 333
           V I+ GG+DFQFYS G+F+G C   L+H VT +GYGE   G  YW++KNSWGT WGE GY
Sbjct: 267 VGIEGGGFDFQFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGY 326

Query: 334 VRMKSNSSDKRGTCGIAMDASYP 341
           +R++ +  DK+G CG+AM ASYP
Sbjct: 327 MRIQKDVKDKQGLCGLAMKASYP 344

[Close]

BLAST of Moc02g04990 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.0e-92
Identity = 172/323 (53.25%), Postives = 214/323 (66.25%), Query Frame = 0


Query: 31  DNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNRSYTLAD 90
           D+   +  + +   Y +W   +    +S  EREKRF +++ NV ++   N  NRSY L  
Sbjct: 24  DDKEIESEEGLSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKL 83

Query: 91  NMFADLTNDEFKTTYLGYLTD----------WSPDTCFKYGNIVNLPTNVDWRKEGAVTP 150
           N FADLT +EFK  Y G               S    + + N+  LP++VDWRK+GAVT 
Sbjct: 84  NKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTE 143

Query: 151 IKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFE 210
           IK+QG+CGSCWAFS VAAVEGI KIKT KLVSLSEQEL+DCD    N+GC+GG M  AFE
Sbjct: 144 IKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVDCDT-KQNEGCNGGLMEIAFE 203

Query: 211 FIKKI-GITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSV 270
           FIKK  GITTE  YPY G++  C+  K      TI G+E VP NDE +L  AVANQPVSV
Sbjct: 204 FIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSV 263

Query: 271 AIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVKNSWGTSWGEYGYVR 330
           AIDAG  DFQFYS G+F+G+CG +LNHGV  VGYG + GK YW+V+NSWG  WGE GY++
Sbjct: 264 AIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIK 323

Query: 331 MKSNSSDKRGTCGIAMDASYPIK 343
           ++    +  G CGIAM+ASYPIK
Sbjct: 324 IEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of Moc02g04990 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.5e-91
Identity = 171/343 (49.85%), Postives = 223/343 (65.01%), Query Frame = 0


Query: 12  FMWLILCVFWTLSMASVAE-DNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQ 71
           F+ L LC+   L      +  N   +  + + + Y++W   +    +S EE+ KRF +++
Sbjct: 4   FIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERW-RSHHTVARSLEEKAKRFNVFK 63

Query: 72  SNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLG--------YLTDWSPDTCFKYGN 131
            NV++I   N  ++SY L  N F D+T++EF+ TY G        +  +      F Y N
Sbjct: 64  HNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATKSFMYAN 123

Query: 132 IVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCD 191
           +  LPT+VDWRK GAVTP+K+QGQCGSCWAFS V AVEGI +I+T KL SLSEQEL+DCD
Sbjct: 124 VNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELVDCD 183

Query: 192 VISGNQGCSGGFMPKAFEFIK-KIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVP 251
             + NQGC+GG M  AFEFIK K G+T+E  YPY+  +  C+  K      +I G+E VP
Sbjct: 184 T-NQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDVP 243

Query: 252 ANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDV-GKS 311
            N E  L  AVANQPVSVAIDAGG DFQFYS G+F+G CG +LNHGV +VGYG  + G  
Sbjct: 244 KNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGTK 303

Query: 312 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 344
           YW+VKNSWG  WGE GY+RM+     K G CGIAM+ASYP+K+
Sbjct: 304 YWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLKN 344

[Close]

BLAST of Moc02g04990 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 329.3 bits (843), Expect = 3.6e-90
Identity = 165/333 (49.55%), Postives = 215/333 (64.56%), Query Frame = 0


Query: 16  ILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQY 75
           +LC  +    + V         +D + + ++ W+ ++ + YKS EE+  RF +++ N+ +
Sbjct: 22  LLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMH 81

Query: 76  IDYFNSLNRSYTLADNMFADLTNDEFKTTYLG-----YLTDWSPDTCFKYGNIVNLPTNV 135
           ID  N+   SY L  N FADLT++EFK  YLG     +     P   F+Y +I +LP +V
Sbjct: 82  IDQRNNEINSYWLGLNEFADLTHEEFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSV 141

Query: 136 DWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGC 195
           DWRK+GAV P+KDQGQCGSCWAFS VAAVEGI +I TG L SLSEQEL+DCD  + N GC
Sbjct: 142 DWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDT-TFNSGC 201

Query: 196 SGGFMPKAFEFIKKI-GITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLK 255
           +GG M  AF++I    G+  E +YPY   E +C +QK      TISGYE VP ND++SL 
Sbjct: 202 NGGLMDYAFQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLV 261

Query: 256 AAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVKNSWG 315
            A+A+QPVSVAI+A G DFQFY GG+F+G CG  L+HGV  VGYG   G  Y +VKNSWG
Sbjct: 262 KALAHQPVSVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWG 321

Query: 316 TSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIK 343
             WGE G++RMK N+    G CGI   ASYP K
Sbjct: 322 PRWGEKGFIRMKRNTGKPEGLCGINKMASYPTK 353

The following BLAST results are available for this feature:

BLAST of Moc02g04990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140756.1	4.6e-204	100.00	ervatamin-B [Momordica charantia]	[more]
XP_038902939.1	3.1e-160	79.59	ervatamin-B [Benincasa hispida]	[more]
XP_038902648.1	6.7e-155	77.33	ervatamin-B-like [Benincasa hispida]	[more]
AKO60151.1	2.8e-153	81.82	cysteine proteinase 1, partial [Citrullus lanatus]	[more]
XP_023513224.1	6.7e-147	74.93	ervatamin-B-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc02g04990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P25803	2.6e-93	51.46	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
P12412	3.4e-93	51.17	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
A2XQE8	6.4e-92	54.49	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=399...	[more]
Q7XWK5	6.4e-92	54.49	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q9FJ47	1.9e-91	51.70	Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]

BLAST of Moc02g04990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CH04	2.2e-204	100.00	ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1	[more]
A0A384S0D9	1.4e-153	81.82	Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1	[more]
A0A6J1FYZ3	9.5e-147	73.76	ervatamin-B-like OS=Cucurbita moschata OX=3662 GN=LOC111449119 PE=3 SV=1	[more]
A0A5A7SQK0	3.6e-146	74.57	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001...	[more]
A0A1S3C828	3.6e-146	74.57	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1	[more]

BLAST of Moc02g04990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G06260.1	1.5e-96	51.45	Cysteine proteinases superfamily protein	[more]
AT5G45890.1	1.3e-92	51.70	senescence-associated gene 12	[more]
AT3G48340.1	5.0e-92	53.25	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	2.5e-91	49.85	Cysteine proteinases superfamily protein	[more]
AT4G35350.1	3.6e-90	49.55	xylem cysteine peptidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 301..307 score: 75.76 coord: 286..296 score: 56.16 coord: 144..159 score: 67.03
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 126..341 e-value: 1.7E-119 score: 413.0
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 126..341 e-value: 3.0E-82 score: 275.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 45..101 e-value: 1.9E-18 score: 77.3
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 45..101 e-value: 1.6E-13 score: 50.9
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 28..343 e-value: 9.3E-118 score: 395.3
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 38..342
None	No IPR available	PANTHER	PTHR12411:SF796	ERVATAMIN-B-LIKE	coord: 38..342
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 284..294
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 301..320
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 144..155
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 127..340 e-value: 2.47677E-108 score: 313.021
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 40..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g04990.1	Moc02g04990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity