Moc02g04540 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g04540
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionNuclear transcription factor Y subunit B-3
Locationchr2: 3368241 .. 3368861 (-)
RNA-Seq ExpressionMoc02g04540
SyntenyMoc02g04540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATTCGGATAACGAATCGGGAGGACACAACAGCAACGCGAACAGCGAGCTATCGGCGAAAGAGCAGGACAGATTCCTGCCAATAGCGAACGTCAGCAGGATCATGAAGAAAGCGCTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAAGAAACGGTACAGGAGTGCGTATCGGAGTTCATCAGCTTCATCACCGGAGAGGCATCGGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGCGACGATTTGTTGTGGGCCATGACGACGCTAGGTTTCGAAGAGTATGTGGAACCGCTCAAGACTTATCTGCAGAAGTACAGGGAAATGGAAGGGGAGAAGAGCACTATGGGGAGACAGGGGGAGAAAGACGGTGGTGGTGGTGGGCCCGGCGGCAGTGGGGCTGGTGGCGGTGGTGGTGGAGGAGTCAACTCTGGTACTGCTGGTGGTGCTGGTGGCGGCGGCTTTAACGGAGTGGGTGGAATGTATGGTGGGATGATGATGATGGAGCATCATCAGGGGGGCGTCTACGGTGGCTCTGGTGGATTTCACCACATGGGCATCGGCGGCGGGAAGGGGGGAAGCGGTGGCTCCGGCACGGGGCACCGATAG

mRNA sequence

ATGGCGGATTCGGATAACGAATCGGGAGGACACAACAGCAACGCGAACAGCGAGCTATCGGCGAAAGAGCAGGACAGATTCCTGCCAATAGCGAACGTCAGCAGGATCATGAAGAAAGCGCTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAAGAAACGGTACAGGAGTGCGTATCGGAGTTCATCAGCTTCATCACCGGAGAGGCATCGGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGCGACGATTTGTTGTGGGCCATGACGACGCTAGGTTTCGAAGAGTATGTGGAACCGCTCAAGACTTATCTGCAGAAGTACAGGGAAATGGAAGGGGAGAAGAGCACTATGGGGAGACAGGGGGAGAAAGACGGTGGTGGTGGTGGGCCCGGCGGCAGTGGGGCTGGTGGCGGTGGTGGTGGAGGAGTCAACTCTGGTACTGCTGGTGGTGCTGGTGGCGGCGGCTTTAACGGAGTGGGTGGAATGTATGGTGGGATGATGATGATGGAGCATCATCAGGGGGGCGTCTACGGTGGCTCTGGTGGATTTCACCACATGGGCATCGGCGGCGGGAAGGGGGGAAGCGGTGGCTCCGGCACGGGGCACCGATAG

Coding sequence (CDS)

ATGGCGGATTCGGATAACGAATCGGGAGGACACAACAGCAACGCGAACAGCGAGCTATCGGCGAAAGAGCAGGACAGATTCCTGCCAATAGCGAACGTCAGCAGGATCATGAAGAAAGCGCTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAAGAAACGGTACAGGAGTGCGTATCGGAGTTCATCAGCTTCATCACCGGAGAGGCATCGGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGCGACGATTTGTTGTGGGCCATGACGACGCTAGGTTTCGAAGAGTATGTGGAACCGCTCAAGACTTATCTGCAGAAGTACAGGGAAATGGAAGGGGAGAAGAGCACTATGGGGAGACAGGGGGAGAAAGACGGTGGTGGTGGTGGGCCCGGCGGCAGTGGGGCTGGTGGCGGTGGTGGTGGAGGAGTCAACTCTGGTACTGCTGGTGGTGCTGGTGGCGGCGGCTTTAACGGAGTGGGTGGAATGTATGGTGGGATGATGATGATGGAGCATCATCAGGGGGGCGTCTACGGTGGCTCTGGTGGATTTCACCACATGGGCATCGGCGGCGGGAAGGGGGGAAGCGGTGGCTCCGGCACGGGGCACCGATAG

Protein sequence

MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR
Homology
BLAST of Moc02g04540 vs. NCBI nr
Match: XP_008464158.1 (PREDICTED: nuclear transcription factor Y subunit B-3 [Cucumis melo] >XP_008464159.1 PREDICTED: nuclear transcription factor Y subunit B-3 [Cucumis melo] >KAA0060811.1 nuclear transcription factor Y subunit B-3 [Cucumis melo var. makuwa] >TYK08821.1 nuclear transcription factor Y subunit B-3 [Cucumis melo var. makuwa])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 191/207 (92.27%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SGTA G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGTAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           YGG+ GFHHMGIGGGKGGSG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGGGKGGSGASGTGHR 200

BLAST of Moc02g04540 vs. NCBI nr
Match: XP_022971106.1 (nuclear transcription factor Y subunit B-3-like [Cucurbita maxima])

HSP 1 Score: 329.3 bits (843), Expect = 2.3e-86
Identity = 196/211 (92.89%), Postives = 199/211 (94.31%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMVHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFHHMGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHHMGIGGGKGGNGGSGTGHR 210

BLAST of Moc02g04540 vs. NCBI nr
Match: XP_022947534.1 (nuclear transcription factor Y subunit B-3-like [Cucurbita moschata] >KAG6604918.1 Nuclear transcription factor Y subunit B-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 326.2 bits (835), Expect = 2.0e-85
Identity = 195/211 (92.42%), Postives = 198/211 (93.84%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMGHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFH MGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHQMGIGGGKGGNGGSGTGHR 210

BLAST of Moc02g04540 vs. NCBI nr
Match: XP_004139999.1 (nuclear transcription factor Y subunit B-3 [Cucumis sativus] >KGN46611.1 hypothetical protein Csa_005340 [Cucumis sativus])

HSP 1 Score: 323.9 bits (829), Expect = 9.8e-85
Identity = 190/208 (91.35%), Postives = 193/208 (92.79%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SG A G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGAAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGG-SGTGHR 207
           YGG+ GFHHMGIG GKGGSGG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGSGKGGSGGASGTGHR 201

BLAST of Moc02g04540 vs. NCBI nr
Match: XP_023532503.1 (nuclear transcription factor Y subunit B-3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323.6 bits (828), Expect = 1.3e-84
Identity = 193/209 (92.34%), Postives = 196/209 (93.78%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHHQG 180
            G +GEKD  GGGGGPGGSGA   GGGGVN GT GG  GGGFNGVGGMYGGMMMM HHQG
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGA-NVGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMGHHQG 180

Query: 181 GVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           GVY GSGGFHHMGIGGGKGG+GGSGTGHR
Sbjct: 181 GVY-GSGGFHHMGIGGGKGGNGGSGTGHR 207

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: O23310 (Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana OX=3702 GN=NFYB3 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.1e-54
Identity = 124/151 (82.12%), Postives = 135/151 (89.40%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDN+SGGH    N+  S +EQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNDSGGHKDGGNA--STREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEK-ST 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+YVEPLK YLQKYRE+EGEK +T
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTT 120

Query: 121 MGRQGEKDGGGGGPGGSGAGGGG----GGGV 147
            GRQG+K+GGGGG GG+G+G GG    GGG+
Sbjct: 121 AGRQGDKEGGGGG-GGAGSGSGGAPMYGGGM 148

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q69J40 (Nuclear transcription factor Y subunit B-10 OS=Oryza sativa subsp. japonica OX=39947 GN=NFYB10 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.0e-44
Identity = 136/220 (61.82%), Postives = 148/220 (67.27%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           M DSDNESGG  SNA    SA+EQDRFLPIANVSRIMK+ALPANAKISKDAKETVQECVS
Sbjct: 1   MPDSDNESGG-PSNAGEYASAREQDRFLPIANVSRIMKRALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+Y++PLK YL K+RE+EGEK+  
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYIDPLKLYLHKFRELEGEKAI- 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGG-----MMMMEHH 180
                   G  G GG GA   GG G  SG+         NG  GMYGG     MMM +  
Sbjct: 121 --------GAAGSGGGGAASSGGSGSGSGSHHHQDASRNNGGYGMYGGGGGMIMMMGQPM 180

Query: 181 QGGVYGGSGGF---------HHMGIGGGKG--GSGGSGTG 205
            G     S G+         HH  + GGKG  G GG G G
Sbjct: 181 YGSPPASSAGYAQPPPPHHHHHQMVMGGKGAYGHGGGGGG 210

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q9FGJ3 (Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana OX=3702 GN=NFYB2 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.0e-44
Identity = 124/207 (59.90%), Postives = 141/207 (68.12%), Query Frame = 0

Query: 1   MADSDNESG----GHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQ 60
           M DSD +SG    G+N N  S LS +EQDRFLPIANVSRIMKKALPANAKISKDAKET+Q
Sbjct: 1   MGDSDRDSGGGQNGNNQNGQSSLSPREQDRFLPIANVSRIMKKALPANAKISKDAKETMQ 60

Query: 61  ECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGE 120
           ECVSEFISF+TGEASDKCQ+EKRKTINGDDLLWAMTTLGFE+YVEPLK YLQ++RE+EGE
Sbjct: 61  ECVSEFISFVTGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGE 120

Query: 121 KSTMGRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGM-YGGMMMMEHH 180
           ++ +GR                  GG  G +   A G GGG + G GGM Y       H 
Sbjct: 121 RTGLGRPQT---------------GGEVGEHQRDAVGDGGGFYGGGGGMQYHQHHQFLHQ 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSG 203
           Q  +YG +GG      G   GG   SG
Sbjct: 181 QNHMYGATGG------GSDSGGGAASG 186

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q75IZ7 (Nuclear transcription factor Y subunit B-8 OS=Oryza sativa subsp. japonica OX=39947 GN=NFYB8 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-40
Identity = 129/215 (60.00%), Postives = 143/215 (66.51%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSA-KEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECV 60
           M DSDN+SGG ++ A  ELS+ +EQDRFLPIANVSRIMKKALPANAKISKDAKETVQECV
Sbjct: 1   MPDSDNDSGGPSNYAGGELSSPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECV 60

Query: 61  SEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKST 120
           SEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+YV+PLK YL K+RE+EGE++ 
Sbjct: 61  SEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVDPLKHYLHKFREIEGERAA 120

Query: 121 MGRQGE-KDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGG--MYGG-----MMM 180
               G         P          GG     A GAG GG   + G  MYG         
Sbjct: 121 ASTTGAGTSAASTTPPQQQHTANAAGGYAGYAAPGAGPGGMMMMMGQPMYGSPPPPPQQQ 180

Query: 181 MEHHQGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
            + H     GG GGF H   GGG G S  SG G +
Sbjct: 181 QQQHHHMAMGGRGGFGHHPGGGGGGSSSSSGHGRQ 215

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q0J7P4 (Nuclear transcription factor Y subunit B-11 OS=Oryza sativa subsp. japonica OX=39947 GN=HD5 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.7e-36
Identity = 113/194 (58.25%), Postives = 131/194 (67.53%), Query Frame = 0

Query: 11  HNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEA 70
           +      +  AKEQDRFLPIANVSRIMK++LPANAKISK++KETVQECVSEFISF+TGEA
Sbjct: 46  YGGGGGGDSPAKEQDRFLPIANVSRIMKRSLPANAKISKESKETVQECVSEFISFVTGEA 105

Query: 71  SDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGEKDGGG 130
           SDKCQREKRKTINGDDLLWAMTTLGFE YV PLK+YL +YRE EGEK+ +          
Sbjct: 106 SDKCQREKRKTINGDDLLWAMTTLGFEAYVGPLKSYLNRYREAEGEKADV---------L 165

Query: 131 GGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEH-HQGGVYGGSGGFHHM 190
           GG GG+ A   G GG   G  GGA G   +G   + GG+    H HQ    GG  G    
Sbjct: 166 GGAGGAAAARHGEGGCCGGGGGGADGVVIDGHYPLAGGLSHSHHGHQQQDGGGDVGLMMG 225

Query: 191 GIGGGKGGSGGSGT 204
           G   G G + G+G+
Sbjct: 226 GGDAGVGYNAGAGS 230

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A1S3CMC5 (nuclear transcription factor Y subunit B-3 OS=Cucumis melo OX=3656 GN=LOC103502105 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.1e-87
Identity = 191/207 (92.27%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SGTA G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGTAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           YGG+ GFHHMGIGGGKGGSG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGGGKGGSGASGTGHR 200

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A5A7V4W8 (Nuclear transcription factor Y subunit B-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00280 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.1e-87
Identity = 191/207 (92.27%), Postives = 194/207 (93.72%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SGTA G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGTAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           YGG+ GFHHMGIGGGKGGSG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGGGKGGSGASGTGHR 200

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A6J1I2E0 (nuclear transcription factor Y subunit B-3-like OS=Cucurbita maxima OX=3661 GN=LOC111469875 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 196/211 (92.89%), Postives = 199/211 (94.31%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMVHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFHHMGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHHMGIGGGKGGNGGSGTGHR 210

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A6J1G731 (nuclear transcription factor Y subunit B-3-like OS=Cucurbita moschata OX=3662 GN=LOC111451370 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 9.5e-86
Identity = 195/211 (92.42%), Postives = 198/211 (93.84%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMGHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFH MGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHQMGIGGGKGGNGGSGTGHR 210

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A0A0KFU2 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G112490 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.7e-85
Identity = 190/208 (91.35%), Postives = 193/208 (92.79%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SG A G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGAAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGG-SGTGHR 207
           YGG+ GFHHMGIG GKGGSGG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGSGKGGSGGASGTGHR 201

BLAST of Moc02g04540 vs. TAIR 10
Match: AT4G14540.1 (nuclear factor Y, subunit B3 )

HSP 1 Score: 211.8 bits (538), Expect = 5.0e-55
Identity = 124/151 (82.12%), Postives = 135/151 (89.40%), Query Frame = 0

Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDN+SGGH    N+  S +EQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNDSGGHKDGGNA--STREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEK-ST 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+YVEPLK YLQKYRE+EGEK +T
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTT 120

Query: 121 MGRQGEKDGGGGGPGGSGAGGGG----GGGV 147
            GRQG+K+GGGGG GG+G+G GG    GGG+
Sbjct: 121 AGRQGDKEGGGGG-GGAGSGSGGAPMYGGGM 148

BLAST of Moc02g04540 vs. TAIR 10
Match: AT5G47640.1 (nuclear factor Y, subunit B2 )

HSP 1 Score: 179.9 bits (455), Expect = 2.1e-45
Identity = 124/207 (59.90%), Postives = 141/207 (68.12%), Query Frame = 0

Query: 1   MADSDNESG----GHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQ 60
           M DSD +SG    G+N N  S LS +EQDRFLPIANVSRIMKKALPANAKISKDAKET+Q
Sbjct: 1   MGDSDRDSGGGQNGNNQNGQSSLSPREQDRFLPIANVSRIMKKALPANAKISKDAKETMQ 60

Query: 61  ECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGE 120
           ECVSEFISF+TGEASDKCQ+EKRKTINGDDLLWAMTTLGFE+YVEPLK YLQ++RE+EGE
Sbjct: 61  ECVSEFISFVTGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGE 120

Query: 121 KSTMGRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGM-YGGMMMMEHH 180
           ++ +GR                  GG  G +   A G GGG + G GGM Y       H 
Sbjct: 121 RTGLGRPQT---------------GGEVGEHQRDAVGDGGGFYGGGGGMQYHQHHQFLHQ 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSG 203
           Q  +YG +GG      G   GG   SG
Sbjct: 181 QNHMYGATGG------GSDSGGGAASG 186

BLAST of Moc02g04540 vs. TAIR 10
Match: AT2G37060.1 (nuclear factor Y, subunit B8 )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0

Query: 6   NESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 65
           +ESGG  S  +  L  +EQDRFLPIAN+SRIMK+ LPAN KI+KDAKE VQECVSEFISF
Sbjct: 15  HESGGDQSPRS--LHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISF 74

Query: 66  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGE 125
           +T EASDKCQREKRKTINGDDLLWAM TLGFE+Y+EPLK YL +YREMEG+     + G+
Sbjct: 75  VTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGD 134

Query: 126 ----KDG 129
               KDG
Sbjct: 135 PNAKKDG 139

BLAST of Moc02g04540 vs. TAIR 10
Match: AT2G37060.2 (nuclear factor Y, subunit B8 )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0

Query: 6   NESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 65
           +ESGG  S  +  L  +EQDRFLPIAN+SRIMK+ LPAN KI+KDAKE VQECVSEFISF
Sbjct: 15  HESGGDQSPRS--LHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISF 74

Query: 66  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGE 125
           +T EASDKCQREKRKTINGDDLLWAM TLGFE+Y+EPLK YL +YREMEG+     + G+
Sbjct: 75  VTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGD 134

Query: 126 ----KDG 129
               KDG
Sbjct: 135 PNAKKDG 139

BLAST of Moc02g04540 vs. TAIR 10
Match: AT2G37060.3 (nuclear factor Y, subunit B8 )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0

Query: 6   NESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 65
           +ESGG  S  +  L  +EQDRFLPIAN+SRIMK+ LPAN KI+KDAKE VQECVSEFISF
Sbjct: 15  HESGGDQSPRS--LHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISF 74

Query: 66  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGE 125
           +T EASDKCQREKRKTINGDDLLWAM TLGFE+Y+EPLK YL +YREMEG+     + G+
Sbjct: 75  VTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGD 134

Query: 126 ----KDG 129
               KDG
Sbjct: 135 PNAKKDG 139

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008464158.11.0e-8692.27PREDICTED: nuclear transcription factor Y subunit B-3 [Cucumis melo] >XP_0084641... [more]
XP_022971106.12.3e-8692.89nuclear transcription factor Y subunit B-3-like [Cucurbita maxima][more]
XP_022947534.12.0e-8592.42nuclear transcription factor Y subunit B-3-like [Cucurbita moschata] >KAG6604918... [more]
XP_004139999.19.8e-8591.35nuclear transcription factor Y subunit B-3 [Cucumis sativus] >KGN46611.1 hypothe... [more]
XP_023532503.11.3e-8492.34nuclear transcription factor Y subunit B-3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O233107.1e-5482.12Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
Q69J401.0e-4461.82Nuclear transcription factor Y subunit B-10 OS=Oryza sativa subsp. japonica OX=3... [more]
Q9FGJ33.0e-4459.90Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
Q75IZ71.5e-4060.00Nuclear transcription factor Y subunit B-8 OS=Oryza sativa subsp. japonica OX=39... [more]
Q0J7P46.7e-3658.25Nuclear transcription factor Y subunit B-11 OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3CMC55.1e-8792.27nuclear transcription factor Y subunit B-3 OS=Cucumis melo OX=3656 GN=LOC1035021... [more]
A0A5A7V4W85.1e-8792.27Nuclear transcription factor Y subunit B-3 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1I2E01.1e-8692.89nuclear transcription factor Y subunit B-3-like OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1G7319.5e-8692.42nuclear transcription factor Y subunit B-3-like OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0KFU24.7e-8591.35CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G1124... [more]
Match NameE-valueIdentityDescription
AT4G14540.15.0e-5582.12nuclear factor Y, subunit B3 [more]
AT5G47640.12.1e-4559.90nuclear factor Y, subunit B2 [more]
AT2G37060.11.3e-4270.08nuclear factor Y, subunit B8 [more]
AT2G37060.21.3e-4270.08nuclear factor Y, subunit B8 [more]
AT2G37060.31.3e-4270.08nuclear factor Y, subunit B8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00615CCAATSUBUNTAcoord: 93..111
score: 44.86
coord: 55..73
score: 50.24
coord: 74..92
score: 38.52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 114..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11064:SF128NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-3coord: 1..181
IPR003958Transcription factor CBF/NF-Y/archaeal histone domainPFAMPF00808CBFD_NFYB_HMFcoord: 27..91
e-value: 2.0E-27
score: 95.2
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 15..131
e-value: 1.2E-47
score: 163.3
IPR009072Histone-foldSUPERFAMILY47113Histone-foldcoord: 24..126
IPR027113Transcription factor NFYB/HAP3PANTHERPTHR11064CCAAT-BINDING TRANSCRIPTION FACTOR-RELATEDcoord: 1..181
IPR003956Transcription factor, NFYB/HAP3, conserved sitePROSITEPS00685NFYB_HAP3coord: 58..74

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g04540.1Moc02g04540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0045944 positive regulation of transcription by RNA polymerase II
cellular_component GO:0016602 CCAAT-binding factor complex
cellular_component GO:0005634 nucleus
molecular_function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
molecular_function GO:0016853 isomerase activity
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding