Moc02g03100 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g03100
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	NADH dehydrogenase subunit 2
Location	chr2: 2359146 .. 2360833 (+)
RNA-Seq Expression	Moc02g03100
Synteny	Moc02g03100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGAAAACGTGACTGAATTGGTCTCGATCACTCTTCGGGACGGAATGGAAGAAGGGAGGAGATTCTCAAACGAGGAAAAGGATCCAATGACTTCGAAAGAATTGAACGAGGAGCCGTATGAGGTGAAAATCTCATGTACGGTTCTGTCGAGTGGCAGTAAGGGTGACTTATCTGTCAACTTTTCCACTATCACTCCCAAAAAACCAAACTCTGCCTTACGTAAAGTTGCCAGAGTACGCTTAACCTCGGGATTTGAAATCACTGCTTGTTTGAATAAGGCTAGAAAGAATGCCCGCCAAGTTCAGATAAGGAAATGCTTCCCCCAACCATTAAAGGAAAGGCTCGACGAAGGGAGGGGAAGGAAAACGCTTTCGGAGATCGAGATTGGATTTCTTTTTCATCAAAAACGAAGAAGGCCGAGGATGGCCTACGGTGCGTGTTATCTGAAGGGAACACGCTTTTTCGACCGCGGTGGTATGATTGTCGGGCCCTCTCCTCGTTCCGCCCGCTGGCCTATTAGGTAGGATAGCTGTCTTCGGGCTTTGCCTGCCCTTTCTCATAAAGAATTCCGGCTTGGCCCGGGAAAACGCTGGCAACAACAGAAAGGAAGGGGTCCATGTAGCTGCTGCGCCCGCCCCCTTCTTAGTCAATGGGGCAGCAGGTTCGGCATCCACTAGAAAAGAGAGAATCCACTTCAGATAACCGCGCCTCTGCGTGGCAGCGTGTGGAATGGCCGAGAGCGGACCTTTTTGGATATATAATCCAAGTCGAGAGTGGAGTTACGGAAACAACCGTCTGATGGAAAACGACTTTCACGTTCGGTTCAGAGAGCACTTTTTTCGTTGAGAATTCCTCGTTCCCTTTCGTGTGAATTCCCCAGCGGCGAATTCGAAACTTGTGGGGCCCTATATAATCTAAGTCGAGAGTGGAGTTACGGAAACAGCCGTCTGATGGAAAACGACTTTCACGTTCGGTTCAGAGAGCACTTTTTTCGTTGAGAATTCCTCGTTCCCTTTCGTGTGAATTCCCCAGCGGCGAATTCGAAACTCGTGGGGCCCTATCTATTCCATCTCTCAAGCCCCGAAGAAAACCCCCCTCCCCTTCGGACTCCATATCTCTTTTGACTCTATATATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACAGCATTCCTTTCTATTGCGCCTAAAATCTCTATTTTTGCTAATATTTCACGTGTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGCAGTATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTCAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACAGGTTTCTCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTATCTTTATTTATGTATTAATGACGATAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTAAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCTCCATTACTATGTTCTCATATGCAGGAATACCCCCGTTAGCCGGTTTTTGTAGCAAATTCTATTTGTTTTTCGCCGCTTTGGGTTGTGGGGCTAGGAATCTGGTTTCAGAAAACACCTAA

mRNA sequence

ATGAATGAAAACGTGACTGAATTGGTCTCGATCACTCTTCGGGACGGAATGGAAGAAGGGAGGAGATTCTCAAACGAGGAAAAGGATCCAATGACTTCGAAAGAATTGAACGAGGAGCCGTATGAGGTGAAAATCTCATGTACGGTTCTGTCGAGTGGCAGTAAGGGTGACTTATCTGTCAACTTTTCCACTATCACTCCCAAAAAACCAAACTCTGCCTTACGTAAAGTTGCCAGAGTACGCTTAACCTCGGGATTTGAAATCACTGCTTGTTTGAATAAGGCTAGAAAGAATGCCCGCCAAGTTCAGATAAGGAAATGCTTCCCCCAACCATTAAAGGAAAGGCTCGACGAAGGGAGGGGAAGGAAAACGCTTTCGGAGATCGAGATTGGATTTCTTTTTCATCAAAAACGAAGAAGGCCGAGGATGGCCTACGGTGCGTGTTATCTGAAGGGAACACGCTTTTTCGACCGCGGTGGTATGATTGTCGGGCCCTCTCCTCGTTCCGCCCGCTGGCCTATTAGTATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTCAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTAAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCTCCATTACTATGTTCTCATATGCAGGAATACCCCCGTTAGCCGGTTTTTGTAGCAAATTCTATTTGTTTTTCGCCGCTTTGGGTTGTGGGGCTAGGAATCTGGTTTCAGAAAACACCTAA

Coding sequence (CDS)

ATGAATGAAAACGTGACTGAATTGGTCTCGATCACTCTTCGGGACGGAATGGAAGAAGGGAGGAGATTCTCAAACGAGGAAAAGGATCCAATGACTTCGAAAGAATTGAACGAGGAGCCGTATGAGGTGAAAATCTCATGTACGGTTCTGTCGAGTGGCAGTAAGGGTGACTTATCTGTCAACTTTTCCACTATCACTCCCAAAAAACCAAACTCTGCCTTACGTAAAGTTGCCAGAGTACGCTTAACCTCGGGATTTGAAATCACTGCTTGTTTGAATAAGGCTAGAAAGAATGCCCGCCAAGTTCAGATAAGGAAATGCTTCCCCCAACCATTAAAGGAAAGGCTCGACGAAGGGAGGGGAAGGAAAACGCTTTCGGAGATCGAGATTGGATTTCTTTTTCATCAAAAACGAAGAAGGCCGAGGATGGCCTACGGTGCGTGTTATCTGAAGGGAACACGCTTTTTCGACCGCGGTGGTATGATTGTCGGGCCCTCTCCTCGTTCCGCCCGCTGGCCTATTAGTATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTCAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGATGCATTCGCCATAGTTTTAGCATTACGGCAAACCCGTGTAAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGCTATTACCTTCTCCATTACTATGTTCTCATATGCAGGAATACCCCCGTTAGCCGGTTTTTGTAGCAAATTCTATTTGTTTTTCGCCGCTTTGGGTTGTGGGGCTAGGAATCTGGTTTCAGAAAACACCTAA

Protein sequence

MNENVTELVSITLRDGMEEGRRFSNEEKDPMTSKELNEEPYEVKISCTVLSSGSKGDLSVNFSTITPKKPNSALRKVARVRLTSGFEITACLNKARKNARQVQIRKCFPQPLKERLDEGRGRKTLSEIEIGFLFHQKRRRPRMAYGACYLKGTRFFDRGGMIVGPSPRSARWPISIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGARNLVSENT

Homology

[Close]

BLAST of Moc02g03100 vs. NCBI nr
Match: CAA0413840.1 (unnamed protein product [Arabidopsis thaliana] >CAD5318298.1 unnamed protein product [Arabidopsis thaliana] >VYS71753.1 unnamed protein product [Arabidopsis thaliana])

HSP 1 Score: 231.9 bits (590), Expect = 6.8e-57
Identity = 140/239 (58.58%), Postives = 143/239 (59.83%), Query Frame = 0


Query: 114 ERLDEGR-----GRKTLSEIEIGFLFHQKRRRPRMAYGACYLKGTRFFDRGGMIVGPSPR 173
           ERLDEGR       K   + EI FL H+KRRRPR+AYGACYLKG RFFDRG MI G SPR
Sbjct: 25  ERLDEGREMRRGKEKAFGDREISFLLHRKRRRPRIAYGACYLKGARFFDRGAMIAGASPR 84

Query: 174 SARWPI------------------------------------------------SIASMI 233
           SARWPI                                                SIASMI
Sbjct: 85  SARWPIGIAACGLCLPIRIIIKNSGSARESAGNNRKEGVHVAAAPAPLLSQWGSSIASMI 144

Query: 234 LGALAAMAQTKVKRPLAHSSIGHV------------------------------DAFAIV 270
           LGALAAMAQTKVKRPLAHSSIGHV                              DAFAIV
Sbjct: 145 LGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIV 204

[Close]

BLAST of Moc02g03100 vs. NCBI nr
Match: CAE5978957.1 (unnamed protein product [Arabidopsis arenosa])

HSP 1 Score: 230.3 bits (586), Expect = 2.0e-56
Identity = 139/239 (58.16%), Postives = 143/239 (59.83%), Query Frame = 0


Query: 114 ERLDEGR-----GRKTLSEIEIGFLFHQKRRRPRMAYGACYLKGTRFFDRGGMIVGPSPR 173
           ERLDEGR       K   + EI FL H+KRRRPR+AYGACYLKG RFFDRG +I G SPR
Sbjct: 25  ERLDEGREMRRGKEKAFGDREISFLLHRKRRRPRIAYGACYLKGARFFDRGAIIAGASPR 84

Query: 174 SARWPI------------------------------------------------SIASMI 233
           SARWPI                                                SIASMI
Sbjct: 85  SARWPIGIAACGLCLPIRIIIKNSGSARESAGNNRKEGVHVAAAPAPLLSQWGSSIASMI 144

Query: 234 LGALAAMAQTKVKRPLAHSSIGHV------------------------------DAFAIV 270
           LGALAAMAQTKVKRPLAHSSIGHV                              DAFAIV
Sbjct: 145 LGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIV 204

[Close]

BLAST of Moc02g03100 vs. NCBI nr
Match: YP_009526573.1 (NADH dehydrogenase subunit 2 [Ammopiptanthus mongolicus] >AXV54332.1 NADH dehydrogenase subunit 2 [Ammopiptanthus mongolicus])

HSP 1 Score: 219.5 bits (558), Expect = 3.5e-53
Identity = 117/157 (74.52%), Postives = 120/157 (76.43%), Query Frame = 0


Query: 143 MAYGACYLKGTRFFDRGGMIVGPSPRSARWPIS--------------------------- 202
           MAYGACY+KGTRFFDRGGMI GPSPRSARWPI                            
Sbjct: 1   MAYGACYMKGTRFFDRGGMIAGPSPRSARWPIRALPALSISARESAGNNRKEGVHVAAAS 60

Query: 203 ---IASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNP 262
              + +   GALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNP
Sbjct: 61  APFLVNGAAGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNP 120

Query: 263 ISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA 270
           ISAITFSITMFSY GIPPLAGFCSKFYLFFAALGCGA
Sbjct: 121 ISAITFSITMFSYVGIPPLAGFCSKFYLFFAALGCGA 157

[Close]

BLAST of Moc02g03100 vs. NCBI nr
Match: KAB8477455.1 (hypothetical protein FH972_025321 [Carpinus fangiana])

HSP 1 Score: 219.2 bits (557), Expect = 4.6e-53
Identity = 128/181 (70.72%), Postives = 134/181 (74.03%), Query Frame = 0


Query: 93  NKARKNARQVQIRKCFPQPLKERLDEGRGRKTLSEIEIGFLFHQKRRRPRMAYG----AC 152
           NKA KNARQVQIRKCF QPLKERLDEGR               + RR    A+G     C
Sbjct: 11  NKAGKNARQVQIRKCFSQPLKERLDEGR---------------EMRRGKENAFGDRDFIC 70

Query: 153 YLKGTRFFDRGGMIVGPSPRSARWPISIASMILGALAAMAQTKVKRPLAHSSIGHVDAFA 212
           +   T+  + G   V     +   P  IASMILGALAAMAQTKVKR LAHSSIGHVDAFA
Sbjct: 71  FSSKTKKAEDGLWCVLSEGNTLFRPRCIASMILGALAAMAQTKVKRLLAHSSIGHVDAFA 130

Query: 213 IVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCG 270
           IVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCG
Sbjct: 131 IVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCG 176

[Close]

BLAST of Moc02g03100 vs. NCBI nr
Match: KYP77876.1 (NADH-ubiquinone oxidoreductase chain 2 [Cajanus cajan])

HSP 1 Score: 198.7 bits (504), Expect = 6.4e-47
Identity = 116/165 (70.30%), Postives = 125/165 (75.76%), Query Frame = 0


Query: 119 GRGRKTLSEIEIGFLFHQKRRRPR-------MAYGACYL--KGTRFFDRGGMIVGP---- 178
           G GRKTLSEI+I F F  K ++          A  A ++   G R+  + G   GP    
Sbjct: 107 GGGRKTLSEIDIFFFFSSKTKKAEDGLRCVLRALPALFILGPGKRWQQQKGR--GPCSCC 166

Query: 179 -SPRSARWPISIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADL 238
             P  ++W  SIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADL
Sbjct: 167 VRPLLSQWGSSIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADL 226

Query: 239 GALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA 270
           GALAKTNPISAITFSITMFSY GIPPLAGFCSKFYLFFAALGCGA
Sbjct: 227 GALAKTNPISAITFSITMFSYVGIPPLAGFCSKFYLFFAALGCGA 269

[Close]

BLAST of Moc02g03100 vs. ExPASy Swiss-Prot
Match: O05000 (NADH-ubiquinone oxidoreductase chain 2 OS=Arabidopsis thaliana OX=3702 GN=ND2 PE=1 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 2.1e-37
Identity = 92/125 (73.60%), Postives = 93/125 (74.40%), Query Frame = 0


Query: 175 SIASMILGALAAMAQTKVKRPLAHSSIGHV------------------------------ 234
           SIASMILGALAAMAQTKVKR LA+SSIGHV                              
Sbjct: 298 SIASMILGALAAMAQTKVKRLLAYSSIGHVGYICIGFSCGTIEGIQSLLIGIFIYALMTM 357

Query: 235 DAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 270
           DAFAIVLALRQTRVKYIADLGALAKTNPI AITFSITMFSYAGIPPLAGFCSKFYLFFAA
Sbjct: 358 DAFAIVLALRQTRVKYIADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAA 417

[Close]

BLAST of Moc02g03100 vs. ExPASy Swiss-Prot
Match: P93401 (NADH-ubiquinone oxidoreductase chain 2 OS=Oenothera berteroana OX=3950 GN=ND2 PE=2 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 2.1e-37
Identity = 93/130 (71.54%), Postives = 95/130 (73.08%), Query Frame = 0


Query: 175 SIASMILGALAAMAQTKVKRPLAHSSIGHV------------------------------ 234
           SIASMILGALAAMAQTKVKR LA+SSIGHV                              
Sbjct: 287 SIASMILGALAAMAQTKVKRLLAYSSIGHVGYICIGFSCGTIEGIQSLLIGLFIYALTTI 346

Query: 235 DAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 275
           +AFAIVLALRQTRVKYIADLGALAKTNPI AITFSITMFSYAGIPPLAGFCSKFYLFFAA
Sbjct: 347 NAFAIVLALRQTRVKYIADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAA 406

[Close]

BLAST of Moc02g03100 vs. ExPASy Swiss-Prot
Match: P26846 (NADH-ubiquinone oxidoreductase chain 2 OS=Marchantia polymorpha OX=3197 GN=ND2 PE=3 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 6.4e-34
Identity = 86/125 (68.80%), Postives = 88/125 (70.40%), Query Frame = 0


Query: 175 SIASMILGALAAMAQTKVKRPLAHSSIGH------------------------------V 234
           SIASMILGALAAMAQ KVKR LA+SSIGH                              V
Sbjct: 288 SIASMILGALAAMAQNKVKRLLAYSSIGHVGYLLIGFSCGTIEGIQSLLIGIFIYVLMTV 347

Query: 235 DAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 270
           + FAIVLALRQ R KYIADLGALAKTNPI AIT SITMFSYAGIPPLAGFCSKFYLFFAA
Sbjct: 348 NVFAIVLALRQNRFKYIADLGALAKTNPILAITLSITMFSYAGIPPLAGFCSKFYLFFAA 407

[Close]

BLAST of Moc02g03100 vs. ExPASy Swiss-Prot
Match: P15688 (NADH-ubiquinone oxidoreductase chain 2 OS=Beta vulgaris OX=161934 GN=ND2 PE=3 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 9.9e-27
Identity = 62/63 (98.41%), Postives = 62/63 (98.41%), Query Frame = 0


Query: 207 FAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALG 266
           FAIV ALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALG
Sbjct: 328 FAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALG 387

Query: 267 CGA 270
           CGA
Sbjct: 388 CGA 390

HSP 2 Score: 59.3 bits (142), Expect = 7.9e-08
Identity = 30/61 (49.18%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 118 EGRGRKTLSEIEIGFLFHQKRRRPRMAYGACYLKGTRFFDRGGMIVGPSPRSARWPISIA 177
           + R RK  +  +  FL    + +        + KGTRFFDRGGMI GPSPRSARWPI IA
Sbjct: 9   KARRRKERAFGDRDFLTFSSKTKKTENVNLSFEKGTRFFDRGGMIFGPSPRSARWPIGIA 68

Query: 178 S 179
           +
Sbjct: 69  A 69

[Close]

BLAST of Moc02g03100 vs. ExPASy Swiss-Prot
Match: Q2W3J7 (NADH-quinone oxidoreductase subunit N OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=nuoN PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.9e-15
Identity = 52/131 (39.69%), Postives = 72/131 (54.96%), Query Frame = 0


Query: 174 ISIASMILGALAAMAQTKVKRPLAHSSIGH------------------------------ 233
           I+I SM +G+ AA+ QT +KR +A+SSIGH                              
Sbjct: 281 IAIGSMFVGSFAAVVQTNIKRLMAYSSIGHVGFVLVGIAAGSTLGIQGVLIYLAIYLFMN 340

Query: 234 VDAFAIVLALRQ--TRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLF 273
           V AFA++L++RQ    V+ I DL  L+KT+P+ A   ++ MFS AG+PPLAGF  KFY+F
Sbjct: 341 VGAFAVILSMRQKGRMVEGIDDLAGLSKTHPMMAFVMAVLMFSMAGVPPLAGFWGKFYVF 400

[Close]

BLAST of Moc02g03100 vs. ExPASy TrEMBL
Match: A0A5S9YIP1 ((thale cress) hypothetical protein OS=Arabidopsis thaliana OX=3702 GN=AN1_LOCUS27127 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 3.3e-57
Identity = 140/239 (58.58%), Postives = 143/239 (59.83%), Query Frame = 0


Query: 114 ERLDEGR-----GRKTLSEIEIGFLFHQKRRRPRMAYGACYLKGTRFFDRGGMIVGPSPR 173
           ERLDEGR       K   + EI FL H+KRRRPR+AYGACYLKG RFFDRG MI G SPR
Sbjct: 25  ERLDEGREMRRGKEKAFGDREISFLLHRKRRRPRIAYGACYLKGARFFDRGAMIAGASPR 84

Query: 174 SARWPI------------------------------------------------SIASMI 233
           SARWPI                                                SIASMI
Sbjct: 85  SARWPIGIAACGLCLPIRIIIKNSGSARESAGNNRKEGVHVAAAPAPLLSQWGSSIASMI 144

Query: 234 LGALAAMAQTKVKRPLAHSSIGHV------------------------------DAFAIV 270
           LGALAAMAQTKVKRPLAHSSIGHV                              DAFAIV
Sbjct: 145 LGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIV 204

[Close]

BLAST of Moc02g03100 vs. ExPASy TrEMBL
Match: A0A385G2A8 (NADH dehydrogenase subunit 2 OS=Ammopiptanthus mongolicus OX=126911 GN=nad2 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.7e-53
Identity = 117/157 (74.52%), Postives = 120/157 (76.43%), Query Frame = 0


Query: 143 MAYGACYLKGTRFFDRGGMIVGPSPRSARWPIS--------------------------- 202
           MAYGACY+KGTRFFDRGGMI GPSPRSARWPI                            
Sbjct: 1   MAYGACYMKGTRFFDRGGMIAGPSPRSARWPIRALPALSISARESAGNNRKEGVHVAAAS 60

Query: 203 ---IASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNP 262
              + +   GALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNP
Sbjct: 61  APFLVNGAAGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADLGALAKTNP 120

Query: 263 ISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA 270
           ISAITFSITMFSY GIPPLAGFCSKFYLFFAALGCGA
Sbjct: 121 ISAITFSITMFSYVGIPPLAGFCSKFYLFFAALGCGA 157

[Close]

BLAST of Moc02g03100 vs. ExPASy TrEMBL
Match: A0A5N6L1P5 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_025321 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 2.2e-53
Identity = 128/181 (70.72%), Postives = 134/181 (74.03%), Query Frame = 0


Query: 93  NKARKNARQVQIRKCFPQPLKERLDEGRGRKTLSEIEIGFLFHQKRRRPRMAYG----AC 152
           NKA KNARQVQIRKCF QPLKERLDEGR               + RR    A+G     C
Sbjct: 11  NKAGKNARQVQIRKCFSQPLKERLDEGR---------------EMRRGKENAFGDRDFIC 70

Query: 153 YLKGTRFFDRGGMIVGPSPRSARWPISIASMILGALAAMAQTKVKRPLAHSSIGHVDAFA 212
           +   T+  + G   V     +   P  IASMILGALAAMAQTKVKR LAHSSIGHVDAFA
Sbjct: 71  FSSKTKKAEDGLWCVLSEGNTLFRPRCIASMILGALAAMAQTKVKRLLAHSSIGHVDAFA 130

Query: 213 IVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCG 270
           IVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCG
Sbjct: 131 IVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCG 176

[Close]

BLAST of Moc02g03100 vs. ExPASy TrEMBL
Match: A0A151UEX8 (NADH-ubiquinone oxidoreductase chain 2 OS=Cajanus cajan OX=3821 GN=KK1_047487 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 3.1e-47
Identity = 116/165 (70.30%), Postives = 125/165 (75.76%), Query Frame = 0


Query: 119 GRGRKTLSEIEIGFLFHQKRRRPR-------MAYGACYL--KGTRFFDRGGMIVGP---- 178
           G GRKTLSEI+I F F  K ++          A  A ++   G R+  + G   GP    
Sbjct: 107 GGGRKTLSEIDIFFFFSSKTKKAEDGLRCVLRALPALFILGPGKRWQQQKGR--GPCSCC 166

Query: 179 -SPRSARWPISIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADL 238
             P  ++W  SIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADL
Sbjct: 167 VRPLLSQWGSSIASMILGALAAMAQTKVKRPLAHSSIGHVDAFAIVLALRQTRVKYIADL 226

Query: 239 GALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA 270
           GALAKTNPISAITFSITMFSY GIPPLAGFCSKFYLFFAALGCGA
Sbjct: 227 GALAKTNPISAITFSITMFSYVGIPPLAGFCSKFYLFFAALGCGA 269

[Close]

BLAST of Moc02g03100 vs. ExPASy TrEMBL
Match: A0A5H2XRW6 (NADH-Ubiquinone/plastoquinone complex I protein OS=Prunus dulcis OX=3755 GN=Prudu_950S000900 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 9.0e-47
Identity = 143/349 (40.97%), Postives = 147/349 (42.12%), Query Frame = 0


Query: 118 EGRGRKTLSEIEIGFLFHQKRRRPRMAYGACYLKGTRFFDRGGMIVGPSPRSARWPI--- 177
           +G GRKTLSEIEI F+FH+KRRRPRMAYGA YL+GTRFFDRG MI GPSPRSARWPI   
Sbjct: 46  KGGGRKTLSEIEILFVFHRKRRRPRMAYGAYYLRGTRFFDRGSMIAGPSPRSARWPIGIA 105

Query: 178 ------------------------------------------------------------ 237
                                                                       
Sbjct: 106 AFGLCLPLKKKFRLGPGKRWQQQKGRGPCSCCARPLLSQWGSRVELREQPSDGKLLSRSV 165

Query: 238 ------------------------------------------------------------ 270
                                                                       
Sbjct: 166 QRALFSLRIPRSLSCKFPCNELKTCGALLSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDI 225

[Close]

BLAST of Moc02g03100 vs. TAIR 10
Match: AT2G07689.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-40
Identity = 94/125 (75.20%), Postives = 94/125 (75.20%), Query Frame = 0


Query: 175 SIASMILGALAAMAQTKVKRPLAHSSIGHV------------------------------ 234
           SIASMILGALAAMAQTKVKRPLAHSSIGHV                              
Sbjct: 51  SIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTM 110

Query: 235 DAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 270
           DAFAIV ALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA
Sbjct: 111 DAFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 170

[Close]

BLAST of Moc02g03100 vs. TAIR 10
Match: ATMG00285.1 (NADH dehydrogenase 2A )

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-40
Identity = 94/125 (75.20%), Postives = 94/125 (75.20%), Query Frame = 0


Query: 175 SIASMILGALAAMAQTKVKRPLAHSSIGHV------------------------------ 234
           SIASMILGALAAMAQTKVKRPLAHSSIGHV                              
Sbjct: 298 SIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTM 357

Query: 235 DAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 270
           DAFAIV ALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA
Sbjct: 358 DAFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 417

[Close]

BLAST of Moc02g03100 vs. TAIR 10
Match: ATMG01320.1 (NADH dehydrogenase 2B )

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-40
Identity = 94/125 (75.20%), Postives = 94/125 (75.20%), Query Frame = 0


Query: 175 SIASMILGALAAMAQTKVKRPLAHSSIGHV------------------------------ 234
           SIASMILGALAAMAQTKVKRPLAHSSIGHV                              
Sbjct: 298 SIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTM 357

Query: 235 DAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 270
           DAFAIV ALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA
Sbjct: 358 DAFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAA 417

[Close]

BLAST of Moc02g03100 vs. TAIR 10
Match: ATCG00065.1 (ribosomal protein S12A )

HSP 1 Score: 54.7 bits (130), Expect = 1.4e-07
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 64 TITPKKPNSALRKVARVRLTSGFEITA 91
          TITPKKPNSALRKVARVRLTSGFEITA
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITA 65

[Close]

BLAST of Moc02g03100 vs. TAIR 10
Match: ATCG01230.1 (ribosomal protein S12B )

HSP 1 Score: 54.7 bits (130), Expect = 1.4e-07
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 0


Query: 64 TITPKKPNSALRKVARVRLTSGFEITA 91
          TITPKKPNSALRKVARVRLTSGFEITA
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITA 65

The following BLAST results are available for this feature:

BLAST of Moc02g03100 vs. NCBI nr


Match Name	E-value	Identity	Description
CAA0413840.1	6.8e-57	58.58	unnamed protein product [Arabidopsis thaliana] >CAD5318298.1 unnamed protein pro...	[more]
CAE5978957.1	2.0e-56	58.16	unnamed protein product [Arabidopsis arenosa]	[more]
YP_009526573.1	3.5e-53	74.52	NADH dehydrogenase subunit 2 [Ammopiptanthus mongolicus] >AXV54332.1 NADH dehydr...	[more]
KAB8477455.1	4.6e-53	70.72	hypothetical protein FH972_025321 [Carpinus fangiana]	[more]
KYP77876.1	6.4e-47	70.30	NADH-ubiquinone oxidoreductase chain 2 [Cajanus cajan]	[more]

BLAST of Moc02g03100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O05000	2.1e-37	73.60	NADH-ubiquinone oxidoreductase chain 2 OS=Arabidopsis thaliana OX=3702 GN=ND2 PE...	[more]
P93401	2.1e-37	71.54	NADH-ubiquinone oxidoreductase chain 2 OS=Oenothera berteroana OX=3950 GN=ND2 PE...	[more]
P26846	6.4e-34	68.80	NADH-ubiquinone oxidoreductase chain 2 OS=Marchantia polymorpha OX=3197 GN=ND2 P...	[more]
P15688	9.9e-27	98.41	NADH-ubiquinone oxidoreductase chain 2 OS=Beta vulgaris OX=161934 GN=ND2 PE=3 SV...	[more]
Q2W3J7	3.9e-15	39.69	NADH-quinone oxidoreductase subunit N OS=Magnetospirillum magneticum (strain AMB...	[more]

BLAST of Moc02g03100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5S9YIP1	3.3e-57	58.58	(thale cress) hypothetical protein OS=Arabidopsis thaliana OX=3702 GN=AN1_LOCUS2...	[more]
A0A385G2A8	1.7e-53	74.52	NADH dehydrogenase subunit 2 OS=Ammopiptanthus mongolicus OX=126911 GN=nad2 PE=4...	[more]
A0A5N6L1P5	2.2e-53	70.72	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_025321 PE=4 SV=1	[more]
A0A151UEX8	3.1e-47	70.30	NADH-ubiquinone oxidoreductase chain 2 OS=Cajanus cajan OX=3821 GN=KK1_047487 PE...	[more]
A0A5H2XRW6	9.0e-47	40.97	NADH-Ubiquinone/plastoquinone complex I protein OS=Prunus dulcis OX=3755 GN=Prud...	[more]

BLAST of Moc02g03100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G07689.1	2.1e-40	75.20	NADH-Ubiquinone/plastoquinone (complex I) protein	[more]
ATMG00285.1	2.1e-40	75.20	NADH dehydrogenase 2A	[more]
ATMG01320.1	2.1e-40	75.20	NADH dehydrogenase 2B	[more]
ATCG00065.1	1.4e-07	100.00	ribosomal protein S12A	[more]
ATCG01230.1	1.4e-07	100.00	ribosomal protein S12B	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006032	Ribosomal protein S12/S23	PRINTS	PR01034	RIBOSOMALS12	coord: 138..150 score: 39.21 coord: 67..82 score: 91.32 coord: 83..102 score: 27.01
IPR006032	Ribosomal protein S12/S23	PFAM	PF00164	Ribosom_S12_S23	coord: 58..92 e-value: 2.2E-9 score: 36.9
IPR006032	Ribosomal protein S12/S23	PROSITE	PS00055	RIBOSOMAL_S12	coord: 68..75
None	No IPR available	GENE3D	2.40.50.140		coord: 11..106 e-value: 2.3E-12 score: 48.6
None	No IPR available	PANTHER	PTHR22773:SF41	NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2	coord: 174..272
None	No IPR available	PANTHER	PTHR22773	NADH DEHYDROGENASE	coord: 174..272
IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit	PFAM	PF00361	Proton_antipo_M	coord: 207..273 e-value: 1.8E-13 score: 50.4 coord: 174..204 e-value: 4.2E-7 score: 29.5
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 60..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g03100.1	Moc02g03100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0015935	small ribosomal subunit
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome