Moc02g02750 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g02750
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionENDO3c domain-containing protein
Locationchr2: 2071798 .. 2075059 (-)
RNA-Seq ExpressionMoc02g02750
SyntenyMoc02g02750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAATTATAGAGATTTATAGGGTGTACAATTCTATTCTATATCTGAGATCCTATAATCAATACAGTGCAAGACTTGCATTACCTGGATTGGCAGAGAAAAGAATGGTTAGTTCAATGGTCCCTGACAAAAGTTTTGAAGGCACCGCTCAAGTGATGAATCCAGAATCAATACTCCAAATCGATGGCAATCCATCTTCAGAATCACGATGTCAAAGCAACAATCGCGAACCTATCATTGAAGAGCCCCAATCACCTGAACCTGCATATGACGAATCACAATTGACAGACATTGAAGATTTATGTGAGTATGACTCTGATGACGTTCCTACAATCAGACTTAGCTCTGGACGGTTCACAACAACTTCACAGAACTGCATGGAAGATAACACTACCAGAGCTTTGGTTCCTGTGCACACCAGAGTTGCTTCCATCCCGATGCCCAAACTGAAGCATATAGATCGCTTGAGGACAGAGCATCAAGCGTAAGTATGCCTTTTTTAGCTTGTACATCTTGGCAAAAAAAAAAAAAAACCTCTGTTTATTTCAATAACTAACAAAACAGATTGCTGAAAGTTGAGTTAAGTGCCACCTGTAAATGGGTGAAGTCACTGTACAAGAGTTATAAAATGTCACTTAAAGCCCACATTAGCCTTTGATAAAATTTTAAATATTGTCTACCTACTTATTCCCTAGTCCTTCACTTGGTCTATTATGGAGTATTAGTTTACGTACAGATTCACTAGGTCTAAAACACATATTATAAAGAAACTAATTGCAACCCACATAATGCTTTATGGTAGATGTAATGTATTTTCCTATGTGTAGCTATGAACTTCCAGATACACATCCTCTCCTATTTGAGGTAAATACTTGACCTCTTGACCTGGGGAATCATTGCCTATATACTTGTGTAGAAGTTTGTTTCTGACCATGTCTTTACAGCTAGAAAGACGAGAGCCAAACGATCCATGCCCGTATCTTTTGTGCATACTGTCACCAGGTACAGCTTGCTGCACAATGTTTTTTTTTTCATTACAAAAATAATAATAATTGAAAGTAATATAGAATATCAAATATCAATGCGTCAATATTATGAGATATAAATCCCACTTCCAATAGTAAGGAAGATCCACGGTTCAAGGTGTAATTGATGTTTTCAGGTGAAACTATAAATTCATCTGAGCCGCCCAATACAAGATGCACGTACAAGGAATTGGGAGAACTTTGTAGTGAGGGATCATGTTCTTCATGCAACACTGTCAGGGAACGAAACAGTGGAATGGTCCGTGGAACAATTTTGGTAAGTGAAAAGCCAAACTTCCAAGTTGTTATTTGACTGCTTTGCAAAATGGCCGATATATAAACCAATTTTACTTCCAGATACCCTGTAGAACAGCCATGAGGGGAAATTTTCCATTAAACGGCACATATTTTCAAGTCAATGAGGTTAGTCAGGATTTAATGTCGGAACTGTTCCACGAGCGATAGCATTGCAACTAAACATATTGAATTCAAATGCACAGGTTTTTGCTGATGATGAATCAAGTAAAAACCCCATTCATGTTCCTAGGGAATGGATATGGGATCTACCTAGGAGAATCACATATTTTGGAACCTCTACAACAACAATTTTTAGAGGTAAATTAACATATGATTGATTCATTAAAGTTCCAATGGAAAATAATCTAATACGTGTTATTACAGGCTTGGCGAAGGAGGATATTCAGTACTGCTTCCAGAAAGGTATGAAGTAACTTAGCTCCAATCCACAAATGAAAGTTTTCTTTCTTGTTTAAAGGAAAAAACCATTGTAATTTTAACGCATATATTGCAGACACTTTTTTTTTTTAACAAGATAAATTGATTAATGAAAATGATTGTTATACAAACTCCAAAAGGAGTGAAAATAAATAACAAACCCTCTAAAGCTCAAGGGGAGTTATAAATGCCTCCCAATTTGAACATATCATACTAACATATCATACTAGGTAAGTAATTTGAAAGGGATATTTGCAGACACTTCTAATAATAGTAGTTAGTAGCAAAAGATCAGCCCCAATTTAGGGGGAGAATCGTCGAGCAGCAGCAGCTGAAATTCATGAATTTCAGCCGTTACAGCAGCCCACGTTCCGGCAGTGTGGTCAGACGTTGCTGCAGTCCATGTCCTCCACACCTCAGCCCCAACCACAACCCAACAATGTTTTTGTCAGATGGTGGAAAGACAAGATCCCAGGTCTCATTCTTTTGAAGAGCATCCTTATTCTTGTTTGTAATTAAAGTATAACATAGCTCGTTTCCAATTCTCATCAGATTTTGCTTTATCGTAAGATAACACATGATTTTTTAGATTTTGAAGACTTGTGCTCAAGCTCTCAAGGTAAGAAAAAGTAGGAACCATAGGAGAAAAATCTCTCAATAGGAGTGTGGATCTTCTTAGATTTGGAGAAGGATAGTTGGGGCCCTGAGACTCAGGAGCTTCAGACAATTGATTGGATACCAGCATTCACTTGCTTATCGTAGAGGCTGTCAGTCTGAGAATGGGCTGTGACGTGGACTGGTCATGCCTGAGCATGGGCTGCTGAAAATCCGAAGATACCATGAATCTTCGGTAATAGTTCACGAGCCCTAAAAACGATCTTAGCTCACTCACCTTGGTAGGTGCTTGCCACTCCTCGATGGCTCTGATGCTCATCCAATGCCCTAGGAATAGGATCTCCGTCTTTACATAGAGGTGATTCTTCCTTAATACCTTACGGTCCGGAGGTGGCTAACGTGGTCGTTTAACGAATAGCTATAGCCCACGATCAAGTATATCACCAGTCGTCTAAGTACGGGTGGAATAGCTCATTGTGGAGGGTGCAGAACGTGGCAGGGGCATTGGTGAGTCCAAAAGGCATAACCAAATCAAATGCCCCTTTATTAGTAAGGTTGCTTGCTTGTCACACAGGTCGTCTTTGGCTCGTCTCCTTCCGCGATACGCACTTGGTAGTTGCCCGACCGTAGATCCAACTTCTCGAAGTATCTCGCGTTTATTGATTAGGGCGAAGAAGTCCGCAATCAAAGTGGATACTTGTTCTTGATGGTTAGGTTACCTTGTTGAGCGCCCGATAATCAATGCACAAGGCTACCGCTTTTTCTGGAATAAATCAAACAAACAGGGCTCCCCAACAAGGGGTTTGGCTCCAGGCTTCCACTCGATCTTGTGGTAGACTGCCCTTCTAGGCTAGGGGGCACTTGGCAACTCACTCGTGGGGCATGA

mRNA sequence

ATGCAAATTATAGAGATTTATAGGGTGTACAATTCTATTCTATATCTGAGATCCTATAATCAATACAGTGCAAGACTTGCATTACCTGGATTGGCAGAGAAAAGAATGGTTAGTTCAATGGTCCCTGACAAAAGTTTTGAAGGCACCGCTCAAGTGATGAATCCAGAATCAATACTCCAAATCGATGGCAATCCATCTTCAGAATCACGATGTCAAAGCAACAATCGCGAACCTATCATTGAAGAGCCCCAATCACCTGAACCTGCATATGACGAATCACAATTGACAGACATTGAAGATTTATGTGAGTATGACTCTGATGACGTTCCTACAATCAGACTTAGCTCTGGACGGTTCACAACAACTTCACAGAACTGCATGGAAGATAACACTACCAGAGCTTTGGTTCCTGTGCACACCAGAGTTGCTTCCATCCCGATGCCCAAACTGAAGCATATAGATCGCTTGAGGACAGAGCATCAAGCCTATGAACTTCCAGATACACATCCTCTCCTATTTGAGCTAGAAAGACGAGAGCCAAACGATCCATGCCCGTATCTTTTGTGCATACTGTCACCAGGTGAAACTATAAATTCATCTGAGCCGCCCAATACAAGATGCACGTACAAGGAATTGGGAGAACTTTGTAGTGAGGGATCATGTTCTTCATGCAACACTGTCAGGGAACGAAACAGTGGAATGGTCCGTGGAACAATTTTGATACCCTGTAGAACAGCCATGAGGGGAAATTTTCCATTAAACGGCACATATTTTCAAGTCAATGAGGTTTTTGCTGATGATGAATCAAGTAAAAACCCCATTCATGTTCCTAGGGAATGGATATGGGATCTACCTAGGAGAATCACATATTTTGGAACCTCTACAACAACAATTTTTAGAGGCTTGGCGAAGGAGGATATTCAGTACTGCTTCCAGAAAGGGCTCCCCAACAAGGGGTTTGGCTCCAGGCTTCCACTCGATCTTGTGGTAGACTGCCCTTCTAGGCTAGGGGGCACTTGGCAACTCACTCGTGGGGCATGA

Coding sequence (CDS)

ATGCAAATTATAGAGATTTATAGGGTGTACAATTCTATTCTATATCTGAGATCCTATAATCAATACAGTGCAAGACTTGCATTACCTGGATTGGCAGAGAAAAGAATGGTTAGTTCAATGGTCCCTGACAAAAGTTTTGAAGGCACCGCTCAAGTGATGAATCCAGAATCAATACTCCAAATCGATGGCAATCCATCTTCAGAATCACGATGTCAAAGCAACAATCGCGAACCTATCATTGAAGAGCCCCAATCACCTGAACCTGCATATGACGAATCACAATTGACAGACATTGAAGATTTATGTGAGTATGACTCTGATGACGTTCCTACAATCAGACTTAGCTCTGGACGGTTCACAACAACTTCACAGAACTGCATGGAAGATAACACTACCAGAGCTTTGGTTCCTGTGCACACCAGAGTTGCTTCCATCCCGATGCCCAAACTGAAGCATATAGATCGCTTGAGGACAGAGCATCAAGCCTATGAACTTCCAGATACACATCCTCTCCTATTTGAGCTAGAAAGACGAGAGCCAAACGATCCATGCCCGTATCTTTTGTGCATACTGTCACCAGGTGAAACTATAAATTCATCTGAGCCGCCCAATACAAGATGCACGTACAAGGAATTGGGAGAACTTTGTAGTGAGGGATCATGTTCTTCATGCAACACTGTCAGGGAACGAAACAGTGGAATGGTCCGTGGAACAATTTTGATACCCTGTAGAACAGCCATGAGGGGAAATTTTCCATTAAACGGCACATATTTTCAAGTCAATGAGGTTTTTGCTGATGATGAATCAAGTAAAAACCCCATTCATGTTCCTAGGGAATGGATATGGGATCTACCTAGGAGAATCACATATTTTGGAACCTCTACAACAACAATTTTTAGAGGCTTGGCGAAGGAGGATATTCAGTACTGCTTCCAGAAAGGGCTCCCCAACAAGGGGTTTGGCTCCAGGCTTCCACTCGATCTTGTGGTAGACTGCCCTTCTAGGCTAGGGGGCACTTGGCAACTCACTCGTGGGGCATGA

Protein sequence

MQIIEIYRVYNSILYLRSYNQYSARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEEPQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKGLPNKGFGSRLPLDLVVDCPSRLGGTWQLTRGA
Homology
BLAST of Moc02g02750 vs. NCBI nr
Match: XP_016902242.1 (PREDICTED: protein ROS1-like isoform X2 [Cucumis melo])

HSP 1 Score: 488.0 bits (1255), Expect = 6.6e-134
Identity = 236/302 (78.15%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPG  EK +VS+M P+KSFEG  Q MN  S+L ID NP SE+   +NN EPIIE 
Sbjct: 1360 SARLALPGPQEKGIVSTMAPEKSFEGNTQAMNSASVLHIDANPFSEA---TNNCEPIIEA 1419

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P SPEPA+DESQLTDIEDL EYDSDDVP IRLSSG+FTTTS NC++D  T+ALVP+HTRV
Sbjct: 1420 PPSPEPAHDESQLTDIEDLYEYDSDDVPIIRLSSGQFTTTSLNCVDDTITKALVPLHTRV 1479

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            AS P+ KLKHI+RLRTEHQAYELPDTHPLL +LERREPNDPCPYLLCILSPGET++S EP
Sbjct: 1480 ASAPLRKLKHIERLRTEHQAYELPDTHPLLSQLERREPNDPCPYLLCILSPGETVDSCEP 1539

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNTRC Y+E GE+C+EGSCSSCN +RE+NSG VRGTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1540 PNTRCVYRETGEICTEGSCSSCNIIREQNSGAVRGTILIPCRTAMRGKFPLNGTYFQVNE 1599

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDE+SKNPI +PREWIW+LPRRI YFGTSTTTIFRGLAKEDIQYCFQKG +  +GF 
Sbjct: 1600 VFADDETSKNPIQIPREWIWNLPRRIAYFGTSTTTIFRGLAKEDIQYCFQKGFICVRGFD 1658

Query: 323  SR 324
             R
Sbjct: 1660 RR 1658

BLAST of Moc02g02750 vs. NCBI nr
Match: XP_008458622.1 (PREDICTED: protein ROS1-like isoform X1 [Cucumis melo] >KAA0033377.1 protein ROS1-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 488.0 bits (1255), Expect = 6.6e-134
Identity = 236/302 (78.15%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPG  EK +VS+M P+KSFEG  Q MN  S+L ID NP SE+   +NN EPIIE 
Sbjct: 1364 SARLALPGPQEKGIVSTMAPEKSFEGNTQAMNSASVLHIDANPFSEA---TNNCEPIIEA 1423

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P SPEPA+DESQLTDIEDL EYDSDDVP IRLSSG+FTTTS NC++D  T+ALVP+HTRV
Sbjct: 1424 PPSPEPAHDESQLTDIEDLYEYDSDDVPIIRLSSGQFTTTSLNCVDDTITKALVPLHTRV 1483

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            AS P+ KLKHI+RLRTEHQAYELPDTHPLL +LERREPNDPCPYLLCILSPGET++S EP
Sbjct: 1484 ASAPLRKLKHIERLRTEHQAYELPDTHPLLSQLERREPNDPCPYLLCILSPGETVDSCEP 1543

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNTRC Y+E GE+C+EGSCSSCN +RE+NSG VRGTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1544 PNTRCVYRETGEICTEGSCSSCNIIREQNSGAVRGTILIPCRTAMRGKFPLNGTYFQVNE 1603

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDE+SKNPI +PREWIW+LPRRI YFGTSTTTIFRGLAKEDIQYCFQKG +  +GF 
Sbjct: 1604 VFADDETSKNPIQIPREWIWNLPRRIAYFGTSTTTIFRGLAKEDIQYCFQKGFICVRGFD 1662

Query: 323  SR 324
             R
Sbjct: 1664 RR 1662

BLAST of Moc02g02750 vs. NCBI nr
Match: XP_022958534.1 (protein ROS1-like [Cucurbita moschata])

HSP 1 Score: 481.5 bits (1238), Expect = 6.2e-132
Identity = 237/302 (78.48%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPGL EKRMVS+M+P+K +EGTAQVMNP  +L I+GNPSSESR ++ N EPIIE 
Sbjct: 1148 SARLALPGLPEKRMVSTMIPEKRYEGTAQVMNPAPVLHIEGNPSSESRYETINCEPIIEV 1207

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P+SPE AYDESQ TDIEDL EYDSDDVP IRL SG+F TTSQNCM++N T AL+P++ RV
Sbjct: 1208 PRSPEHAYDESQSTDIEDLYEYDSDDVPVIRLDSGQF-TTSQNCMDNNITGALIPLNNRV 1267

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            ASIPM KLKH+DRLRTEHQAYELPDTHPLL+ELE RE ND CPYLLCI SPGETI+SSEP
Sbjct: 1268 ASIPMRKLKHVDRLRTEHQAYELPDTHPLLYELEPRELNDACPYLLCIWSPGETIDSSEP 1327

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNT+CTY+E GELCSEGSCSSCN +R++NSG V GTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1328 PNTKCTYQETGELCSEGSCSSCNILRKQNSGTVHGTILIPCRTAMRGKFPLNGTYFQVNE 1387

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDESSKNPI V REWIWDLPRRI YFGTST TIFRGL  +DIQYCFQKG +  +GF 
Sbjct: 1388 VFADDESSKNPISVFREWIWDLPRRIVYFGTSTATIFRGLGIDDIQYCFQKGFICVRGFD 1447

Query: 323  SR 324
             R
Sbjct: 1448 RR 1448

BLAST of Moc02g02750 vs. NCBI nr
Match: XP_023532993.1 (protein ROS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 480.3 bits (1235), Expect = 1.4e-131
Identity = 237/302 (78.48%), Postives = 262/302 (86.75%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPGL EKRMVS+M+P+K +EGTAQVMNP  +L I+GNPSSESR ++ N EPIIE 
Sbjct: 1005 SARLALPGLPEKRMVSTMIPEKRYEGTAQVMNPAPVLHIEGNPSSESRYETINCEPIIEV 1064

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P+SPE AYDESQ TDIEDL EYDSDDVP IRL SGR  TTSQNCM++N T AL+P++ RV
Sbjct: 1065 PRSPEHAYDESQSTDIEDLYEYDSDDVPVIRLDSGR-VTTSQNCMDNNITGALIPLNNRV 1124

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            ASIPM KLKH+DRLRTEHQAYELPDTHPLL+ELE RE ND CPYLLCI SPGETI+SSEP
Sbjct: 1125 ASIPMRKLKHVDRLRTEHQAYELPDTHPLLYELEPRELNDACPYLLCIWSPGETIDSSEP 1184

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNT+CTY+E GELCSEGSCSSCN +R++NSG V GTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1185 PNTKCTYQETGELCSEGSCSSCNILRKQNSGTVHGTILIPCRTAMRGKFPLNGTYFQVNE 1244

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDESSKNPI V REWIWDLPRRI YFGTST TIFRGL  +DIQYCFQKG +  +GF 
Sbjct: 1245 VFADDESSKNPISVFREWIWDLPRRIVYFGTSTATIFRGLGIDDIQYCFQKGFICVRGFD 1304

Query: 323  SR 324
             R
Sbjct: 1305 RR 1305

BLAST of Moc02g02750 vs. NCBI nr
Match: XP_022996087.1 (protein ROS1-like [Cucurbita maxima])

HSP 1 Score: 474.2 bits (1219), Expect = 9.8e-130
Identity = 232/302 (76.82%), Postives = 261/302 (86.42%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPGL EKRMVS+M+P+K +EGTAQVMNP  +L ++GNPSSESR ++ N EPIIE 
Sbjct: 1116 SARLALPGLPEKRMVSTMIPEKRYEGTAQVMNPAPVLHVEGNPSSESRYETINCEPIIEV 1175

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P+SPE AYDESQ TDIEDL EYDSDDVP IRL SG+F TTSQNCM+++ T AL+P++ RV
Sbjct: 1176 PRSPEHAYDESQSTDIEDLYEYDSDDVPVIRLDSGQF-TTSQNCMDNSITGALIPLNDRV 1235

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            ASIPM KLKH+DRLRTEHQ YELPDTHPLL+ELE RE ND CPYLLCI SPGET++SSEP
Sbjct: 1236 ASIPMRKLKHVDRLRTEHQVYELPDTHPLLYELEPREINDACPYLLCIWSPGETVDSSEP 1295

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNT+CTY+  GELCSEGSCSSCN +R++NSG V GTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1296 PNTKCTYQGTGELCSEGSCSSCNILRKQNSGTVHGTILIPCRTAMRGKFPLNGTYFQVNE 1355

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDESSKNPI V REWIWDLPRRI YFGTST TIFRGL  +DIQYCFQKG +  +GF 
Sbjct: 1356 VFADDESSKNPISVFREWIWDLPRRIVYFGTSTATIFRGLGIDDIQYCFQKGFICVRGFD 1415

Query: 323  SR 324
             R
Sbjct: 1416 RR 1416

BLAST of Moc02g02750 vs. ExPASy Swiss-Prot
Match: C7IW64 (Protein ROS1A OS=Oryza sativa subsp. japonica OX=39947 GN=ROS1A PE=1 SV=2)

HSP 1 Score: 312.0 bits (798), Expect = 8.5e-84
Identity = 161/304 (52.96%), Postives = 212/304 (69.74%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVP--DKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPII 82
            SARLALPG  EK +V+S  P   ++F  T  + +   + Q++ N S+      NNR+PII
Sbjct: 1607 SARLALPGPEEKSLVTSGTPIAAETFHQT-YISSRPVVSQLEWN-SNTCHHGMNNRQPII 1666

Query: 83   EEPQSPEPAYDESQLTD--IEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNT------- 142
            EEP SPEP ++  ++ +  IED    D +++PTI+L+   FT   ++ M+ N        
Sbjct: 1667 EEPASPEPEHETEEMKECAIEDSFVDDPEEIPTIKLNFEEFTQNLKSYMQANNIEIEDAD 1726

Query: 143  -TRALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCI 202
             ++ALV +   VASIP PKLK++ RLRTEHQ YELPD+HPLL    +REP+DPCPYLL I
Sbjct: 1727 MSKALVAITPEVASIPTPKLKNVSRLRTEHQVYELPDSHPLLEGFNQREPDDPCPYLLSI 1786

Query: 203  LSPGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGN 262
             +PGET  S++ P + C  +E GELC+  +C SCN++RE  +  VRGT+LIPCRTAMRG+
Sbjct: 1787 WTPGETAQSTDAPKSVCNSQENGELCASNTCFSCNSIREAQAQKVRGTLLIPCRTAMRGS 1846

Query: 263  FPLNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYC 315
            FPLNGTYFQVNEVFAD +SS+NPI VPR WIW+LPRR  YFGTS  TIF+GL  E+IQ+C
Sbjct: 1847 FPLNGTYFQVNEVFADHDSSRNPIDVPRSWIWNLPRRTVYFGTSIPTIFKGLTTEEIQHC 1906

BLAST of Moc02g02750 vs. ExPASy Swiss-Prot
Match: Q8LK56 (Transcriptional activator DEMETER OS=Arabidopsis thaliana OX=3702 GN=DME PE=1 SV=2)

HSP 1 Score: 304.3 bits (778), Expect = 1.8e-81
Identity = 163/302 (53.97%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSM--VPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPII 82
            SARLALP   E+ + S+   VP +S+   A  M  E  L ++ + +S +     N EPII
Sbjct: 1654 SARLALPAPEERSLTSATIPVPPESYPPVAIPM-IELPLPLEKSLASGAPSNRENCEPII 1713

Query: 83   EEPQSPEPAYDESQLTDIED-LCEYDSDDVPTIRLSSGRFTTTSQNCMEDN-------TT 142
            EEP SP     E   +DIED     D D++PTI+L+  +F  T +  ME N        +
Sbjct: 1714 EEPASPGQECTEITESDIEDAYYNEDPDEIPTIKLNIEQFGMTLREHMERNMELQEGDMS 1773

Query: 143  RALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILS 202
            +ALV +H    SIP PKLK+I RLRTEHQ YELPD+H LL  +++REP+DP PYLL I +
Sbjct: 1774 KALVALHPTTTSIPTPKLKNISRLRTEHQVYELPDSHRLLDGMDKREPDDPSPYLLAIWT 1833

Query: 203  PGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFP 262
            PGET NS++PP  +C  K  G++C + +CS CN++RE NS  VRGT+LIPCRTAMRG+FP
Sbjct: 1834 PGETANSAQPPEQKCGGKASGKMCFDETCSECNSLREANSQTVRGTLLIPCRTAMRGSFP 1893

Query: 263  LNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQ 315
            LNGTYFQVNE+FAD ESS  PI VPR+WIWDLPRR  YFGTS T+IFRGL+ E IQ+CF 
Sbjct: 1894 LNGTYFQVNELFADHESSLKPIDVPRDWIWDLPRRTVYFGTSVTSIFRGLSTEQIQFCFW 1953

BLAST of Moc02g02750 vs. ExPASy Swiss-Prot
Match: Q9SJQ6 (DNA glycosylase/AP lyase ROS1 OS=Arabidopsis thaliana OX=3702 GN=ROS1 PE=1 SV=2)

HSP 1 Score: 293.5 bits (750), Expect = 3.1e-78
Identity = 162/302 (53.64%), Postives = 201/302 (66.56%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGN---PSSESRCQSNNREPI 82
            SARLALP   EK M +   PDK+      +  PE   +  G+     SE   +    EPI
Sbjct: 1063 SARLALPS-TEKGMGT---PDKN---PLPLHLPEPFQREQGSEVVQHSEPAKKVTCCEPI 1122

Query: 83   IEEPQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCME-------DNTT 142
            IEEP SPEP   E  + DIE+    D +++PTIRL+   FT+  +  ME        N +
Sbjct: 1123 IEEPASPEPETAEVSIADIEEAFFEDPEEIPTIRLNMDAFTSNLKKIMEHNKELQDGNMS 1182

Query: 143  RALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILS 202
             ALV +    AS+PMPKLK+I +LRTEH+ YELPD HPLL +LE+REP+DPC YLL I +
Sbjct: 1183 SALVALTAETASLPMPKLKNISQLRTEHRVYELPDEHPLLAQLEKREPDDPCSYLLAIWT 1242

Query: 203  PGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFP 262
            PGET +S +P  + C ++  G LC E +C SCN+++E  S +VRGTILIPCRTAMRG+FP
Sbjct: 1243 PGETADSIQPSVSTCIFQANGMLCDEETCFSCNSIKETRSQIVRGTILIPCRTAMRGSFP 1302

Query: 263  LNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQ 315
            LNGTYFQVNEVFAD  SS NPI+VPRE IW+LPRR  YFGTS  TIF+GL+ E IQ CF 
Sbjct: 1303 LNGTYFQVNEVFADHASSLNPINVPRELIWELPRRTVYFGTSVPTIFKGLSTEKIQACFW 1357

BLAST of Moc02g02750 vs. ExPASy Swiss-Prot
Match: B8YIE8 (Protein ROS1C OS=Oryza sativa subsp. japonica OX=39947 GN=ROS1C PE=2 SV=2)

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-72
Identity = 148/303 (48.84%), Postives = 195/303 (64.36%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALP   +KR+V+         GT    N   + Q++G+  +     +NN  PIIEE
Sbjct: 1517 SARLALPSPQDKRLVNLSNQFAFHNGTMPTPNSTPLPQLEGSIHARD-VHANNTNPIIEE 1576

Query: 83   PQSP-EPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCME--------DNTTR 142
            P SP E    E    DIED  E D+D++P I+L+   F+   +NC++        D+ T+
Sbjct: 1577 PASPREEECRELLENDIEDFDE-DTDEIPIIKLNMEAFSQNLENCIKESNKDFQSDDITK 1636

Query: 143  ALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLL--FELERREPNDPCPYLLCIL 202
            ALV +    ASIP+PKLK++ RLRTEH  YELPD+HPL+    L++REP+DP PYLL I 
Sbjct: 1637 ALVAISNEAASIPVPKLKNVHRLRTEHYVYELPDSHPLMQQLALDQREPDDPSPYLLAIW 1696

Query: 203  SPGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNF 262
            +P E  ++ E P   C  +  G LCS   C +C + RE     VRGT+L+PCRTAMRG+F
Sbjct: 1697 TPDELKDTREAPKPCCNPQTEGGLCSNEMCHNCVSERENQYRYVRGTVLVPCRTAMRGSF 1756

Query: 263  PLNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCF 315
            PLNGTYFQVNEVFAD  SS NPI++PRE +W+L RR+ YFGTS  TIF+GL  E+IQ+CF
Sbjct: 1757 PLNGTYFQVNEVFADHSSSHNPINIPREQLWNLHRRMVYFGTSVPTIFKGLTTEEIQHCF 1816

BLAST of Moc02g02750 vs. ExPASy Swiss-Prot
Match: Q9SR66 (DEMETER-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=DML2 PE=3 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 7.5e-64
Identity = 147/306 (48.04%), Postives = 185/306 (60.46%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALP   E    S M+ ++  +    V+N    L +      E++ +S N EPIIEE
Sbjct: 995  SARLALPEPEESDRTSVMIHERRSKRKPVVVNFRPSLFLYQEKEQEAQ-RSQNCEPIIEE 1054

Query: 83   PQSPEPAYDESQLTDI----------EDLCEYDSDDVPTIRLSSGRFTTTSQNCM---ED 142
            P SPEP Y E  + D           ED  E + D +PTI L+  +   TS + +   E 
Sbjct: 1055 PASPEPEYIEHDIEDYPRDKNNVGTSEDPWE-NKDVIPTIILN--KEAGTSHDLVVNKEA 1114

Query: 143  NTTRALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLC 202
             T+  LV + T  A+IP  KLK  ++LRTEH  +ELPD H +L   ERRE  D  PYLL 
Sbjct: 1115 GTSHDLVVLSTYAAAIPRRKLKIKEKLRTEHHVFELPDHHSILEGFERREAEDIVPYLLA 1174

Query: 203  ILSPGETINSSEPPNTRCT-YKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMR 262
            I +PGET+NS +PP  RC  ++    LC+E  C  CN  RE  S  VRGTILIPCRTAMR
Sbjct: 1175 IWTPGETVNSIQPPKQRCALFESNNTLCNENKCFQCNKTREEESQTVRGTILIPCRTAMR 1234

Query: 263  GNFPLNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQ 315
            G FPLNGTYFQ NEVFAD +SS NPI VP E IWDL RR+ Y G+S ++I +GL+ E I+
Sbjct: 1235 GGFPLNGTYFQTNEVFADHDSSINPIDVPTELIWDLKRRVAYLGSSVSSICKGLSVEAIK 1294

BLAST of Moc02g02750 vs. ExPASy TrEMBL
Match: A0A5A7SVP4 (Protein ROS1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00270 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.2e-134
Identity = 236/302 (78.15%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPG  EK +VS+M P+KSFEG  Q MN  S+L ID NP SE+   +NN EPIIE 
Sbjct: 1364 SARLALPGPQEKGIVSTMAPEKSFEGNTQAMNSASVLHIDANPFSEA---TNNCEPIIEA 1423

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P SPEPA+DESQLTDIEDL EYDSDDVP IRLSSG+FTTTS NC++D  T+ALVP+HTRV
Sbjct: 1424 PPSPEPAHDESQLTDIEDLYEYDSDDVPIIRLSSGQFTTTSLNCVDDTITKALVPLHTRV 1483

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            AS P+ KLKHI+RLRTEHQAYELPDTHPLL +LERREPNDPCPYLLCILSPGET++S EP
Sbjct: 1484 ASAPLRKLKHIERLRTEHQAYELPDTHPLLSQLERREPNDPCPYLLCILSPGETVDSCEP 1543

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNTRC Y+E GE+C+EGSCSSCN +RE+NSG VRGTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1544 PNTRCVYRETGEICTEGSCSSCNIIREQNSGAVRGTILIPCRTAMRGKFPLNGTYFQVNE 1603

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDE+SKNPI +PREWIW+LPRRI YFGTSTTTIFRGLAKEDIQYCFQKG +  +GF 
Sbjct: 1604 VFADDETSKNPIQIPREWIWNLPRRIAYFGTSTTTIFRGLAKEDIQYCFQKGFICVRGFD 1662

Query: 323  SR 324
             R
Sbjct: 1664 RR 1662

BLAST of Moc02g02750 vs. ExPASy TrEMBL
Match: A0A1S4E1Z0 (protein ROS1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497972 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.2e-134
Identity = 236/302 (78.15%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPG  EK +VS+M P+KSFEG  Q MN  S+L ID NP SE+   +NN EPIIE 
Sbjct: 1360 SARLALPGPQEKGIVSTMAPEKSFEGNTQAMNSASVLHIDANPFSEA---TNNCEPIIEA 1419

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P SPEPA+DESQLTDIEDL EYDSDDVP IRLSSG+FTTTS NC++D  T+ALVP+HTRV
Sbjct: 1420 PPSPEPAHDESQLTDIEDLYEYDSDDVPIIRLSSGQFTTTSLNCVDDTITKALVPLHTRV 1479

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            AS P+ KLKHI+RLRTEHQAYELPDTHPLL +LERREPNDPCPYLLCILSPGET++S EP
Sbjct: 1480 ASAPLRKLKHIERLRTEHQAYELPDTHPLLSQLERREPNDPCPYLLCILSPGETVDSCEP 1539

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNTRC Y+E GE+C+EGSCSSCN +RE+NSG VRGTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1540 PNTRCVYRETGEICTEGSCSSCNIIREQNSGAVRGTILIPCRTAMRGKFPLNGTYFQVNE 1599

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDE+SKNPI +PREWIW+LPRRI YFGTSTTTIFRGLAKEDIQYCFQKG +  +GF 
Sbjct: 1600 VFADDETSKNPIQIPREWIWNLPRRIAYFGTSTTTIFRGLAKEDIQYCFQKGFICVRGFD 1658

Query: 323  SR 324
             R
Sbjct: 1660 RR 1658

BLAST of Moc02g02750 vs. ExPASy TrEMBL
Match: A0A1S3C8A0 (protein ROS1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497972 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.2e-134
Identity = 236/302 (78.15%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPG  EK +VS+M P+KSFEG  Q MN  S+L ID NP SE+   +NN EPIIE 
Sbjct: 1364 SARLALPGPQEKGIVSTMAPEKSFEGNTQAMNSASVLHIDANPFSEA---TNNCEPIIEA 1423

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P SPEPA+DESQLTDIEDL EYDSDDVP IRLSSG+FTTTS NC++D  T+ALVP+HTRV
Sbjct: 1424 PPSPEPAHDESQLTDIEDLYEYDSDDVPIIRLSSGQFTTTSLNCVDDTITKALVPLHTRV 1483

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            AS P+ KLKHI+RLRTEHQAYELPDTHPLL +LERREPNDPCPYLLCILSPGET++S EP
Sbjct: 1484 ASAPLRKLKHIERLRTEHQAYELPDTHPLLSQLERREPNDPCPYLLCILSPGETVDSCEP 1543

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNTRC Y+E GE+C+EGSCSSCN +RE+NSG VRGTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1544 PNTRCVYRETGEICTEGSCSSCNIIREQNSGAVRGTILIPCRTAMRGKFPLNGTYFQVNE 1603

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDE+SKNPI +PREWIW+LPRRI YFGTSTTTIFRGLAKEDIQYCFQKG +  +GF 
Sbjct: 1604 VFADDETSKNPIQIPREWIWNLPRRIAYFGTSTTTIFRGLAKEDIQYCFQKGFICVRGFD 1662

Query: 323  SR 324
             R
Sbjct: 1664 RR 1662

BLAST of Moc02g02750 vs. ExPASy TrEMBL
Match: A0A6J1H5D5 (protein ROS1-like OS=Cucurbita moschata OX=3662 GN=LOC111459738 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 3.0e-132
Identity = 237/302 (78.48%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPGL EKRMVS+M+P+K +EGTAQVMNP  +L I+GNPSSESR ++ N EPIIE 
Sbjct: 1148 SARLALPGLPEKRMVSTMIPEKRYEGTAQVMNPAPVLHIEGNPSSESRYETINCEPIIEV 1207

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P+SPE AYDESQ TDIEDL EYDSDDVP IRL SG+F TTSQNCM++N T AL+P++ RV
Sbjct: 1208 PRSPEHAYDESQSTDIEDLYEYDSDDVPVIRLDSGQF-TTSQNCMDNNITGALIPLNNRV 1267

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            ASIPM KLKH+DRLRTEHQAYELPDTHPLL+ELE RE ND CPYLLCI SPGETI+SSEP
Sbjct: 1268 ASIPMRKLKHVDRLRTEHQAYELPDTHPLLYELEPRELNDACPYLLCIWSPGETIDSSEP 1327

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNT+CTY+E GELCSEGSCSSCN +R++NSG V GTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1328 PNTKCTYQETGELCSEGSCSSCNILRKQNSGTVHGTILIPCRTAMRGKFPLNGTYFQVNE 1387

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDESSKNPI V REWIWDLPRRI YFGTST TIFRGL  +DIQYCFQKG +  +GF 
Sbjct: 1388 VFADDESSKNPISVFREWIWDLPRRIVYFGTSTATIFRGLGIDDIQYCFQKGFICVRGFD 1447

Query: 323  SR 324
             R
Sbjct: 1448 RR 1448

BLAST of Moc02g02750 vs. ExPASy TrEMBL
Match: A0A6J1K0Y6 (protein ROS1-like OS=Cucurbita maxima OX=3661 GN=LOC111491404 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.8e-130
Identity = 232/302 (76.82%), Postives = 261/302 (86.42%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALPGL EKRMVS+M+P+K +EGTAQVMNP  +L ++GNPSSESR ++ N EPIIE 
Sbjct: 1116 SARLALPGLPEKRMVSTMIPEKRYEGTAQVMNPAPVLHVEGNPSSESRYETINCEPIIEV 1175

Query: 83   PQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCMEDNTTRALVPVHTRV 142
            P+SPE AYDESQ TDIEDL EYDSDDVP IRL SG+F TTSQNCM+++ T AL+P++ RV
Sbjct: 1176 PRSPEHAYDESQSTDIEDLYEYDSDDVPVIRLDSGQF-TTSQNCMDNSITGALIPLNDRV 1235

Query: 143  ASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILSPGETINSSEP 202
            ASIPM KLKH+DRLRTEHQ YELPDTHPLL+ELE RE ND CPYLLCI SPGET++SSEP
Sbjct: 1236 ASIPMRKLKHVDRLRTEHQVYELPDTHPLLYELEPREINDACPYLLCIWSPGETVDSSEP 1295

Query: 203  PNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFPLNGTYFQVNE 262
            PNT+CTY+  GELCSEGSCSSCN +R++NSG V GTILIPCRTAMRG FPLNGTYFQVNE
Sbjct: 1296 PNTKCTYQGTGELCSEGSCSSCNILRKQNSGTVHGTILIPCRTAMRGKFPLNGTYFQVNE 1355

Query: 263  VFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQKG-LPNKGFG 322
            VFADDESSKNPI V REWIWDLPRRI YFGTST TIFRGL  +DIQYCFQKG +  +GF 
Sbjct: 1356 VFADDESSKNPISVFREWIWDLPRRIVYFGTSTATIFRGLGIDDIQYCFQKGFICVRGFD 1415

Query: 323  SR 324
             R
Sbjct: 1416 RR 1416

BLAST of Moc02g02750 vs. TAIR 10
Match: AT5G04560.1 (HhH-GPD base excision DNA repair family protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 163/302 (53.97%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSM--VPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPII 82
            SARLALP   E+ + S+   VP +S+   A  M  E  L ++ + +S +     N EPII
Sbjct: 1396 SARLALPAPEERSLTSATIPVPPESYPPVAIPM-IELPLPLEKSLASGAPSNRENCEPII 1455

Query: 83   EEPQSPEPAYDESQLTDIED-LCEYDSDDVPTIRLSSGRFTTTSQNCMEDN-------TT 142
            EEP SP     E   +DIED     D D++PTI+L+  +F  T +  ME N        +
Sbjct: 1456 EEPASPGQECTEITESDIEDAYYNEDPDEIPTIKLNIEQFGMTLREHMERNMELQEGDMS 1515

Query: 143  RALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILS 202
            +ALV +H    SIP PKLK+I RLRTEHQ YELPD+H LL  +++REP+DP PYLL I +
Sbjct: 1516 KALVALHPTTTSIPTPKLKNISRLRTEHQVYELPDSHRLLDGMDKREPDDPSPYLLAIWT 1575

Query: 203  PGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFP 262
            PGET NS++PP  +C  K  G++C + +CS CN++RE NS  VRGT+LIPCRTAMRG+FP
Sbjct: 1576 PGETANSAQPPEQKCGGKASGKMCFDETCSECNSLREANSQTVRGTLLIPCRTAMRGSFP 1635

Query: 263  LNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQ 315
            LNGTYFQVNE+FAD ESS  PI VPR+WIWDLPRR  YFGTS T+IFRGL+ E IQ+CF 
Sbjct: 1636 LNGTYFQVNELFADHESSLKPIDVPRDWIWDLPRRTVYFGTSVTSIFRGLSTEQIQFCFW 1695

BLAST of Moc02g02750 vs. TAIR 10
Match: AT5G04560.2 (HhH-GPD base excision DNA repair family protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 163/302 (53.97%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSM--VPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPII 82
            SARLALP   E+ + S+   VP +S+   A  M  E  L ++ + +S +     N EPII
Sbjct: 1654 SARLALPAPEERSLTSATIPVPPESYPPVAIPM-IELPLPLEKSLASGAPSNRENCEPII 1713

Query: 83   EEPQSPEPAYDESQLTDIED-LCEYDSDDVPTIRLSSGRFTTTSQNCMEDN-------TT 142
            EEP SP     E   +DIED     D D++PTI+L+  +F  T +  ME N        +
Sbjct: 1714 EEPASPGQECTEITESDIEDAYYNEDPDEIPTIKLNIEQFGMTLREHMERNMELQEGDMS 1773

Query: 143  RALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILS 202
            +ALV +H    SIP PKLK+I RLRTEHQ YELPD+H LL  +++REP+DP PYLL I +
Sbjct: 1774 KALVALHPTTTSIPTPKLKNISRLRTEHQVYELPDSHRLLDGMDKREPDDPSPYLLAIWT 1833

Query: 203  PGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFP 262
            PGET NS++PP  +C  K  G++C + +CS CN++RE NS  VRGT+LIPCRTAMRG+FP
Sbjct: 1834 PGETANSAQPPEQKCGGKASGKMCFDETCSECNSLREANSQTVRGTLLIPCRTAMRGSFP 1893

Query: 263  LNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQ 315
            LNGTYFQVNE+FAD ESS  PI VPR+WIWDLPRR  YFGTS T+IFRGL+ E IQ+CF 
Sbjct: 1894 LNGTYFQVNELFADHESSLKPIDVPRDWIWDLPRRTVYFGTSVTSIFRGLSTEQIQFCFW 1953

BLAST of Moc02g02750 vs. TAIR 10
Match: AT2G36490.1 (demeter-like 1 )

HSP 1 Score: 293.5 bits (750), Expect = 2.2e-79
Identity = 162/302 (53.64%), Postives = 201/302 (66.56%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGN---PSSESRCQSNNREPI 82
            SARLALP   EK M +   PDK+      +  PE   +  G+     SE   +    EPI
Sbjct: 1063 SARLALPS-TEKGMGT---PDKN---PLPLHLPEPFQREQGSEVVQHSEPAKKVTCCEPI 1122

Query: 83   IEEPQSPEPAYDESQLTDIEDLCEYDSDDVPTIRLSSGRFTTTSQNCME-------DNTT 142
            IEEP SPEP   E  + DIE+    D +++PTIRL+   FT+  +  ME        N +
Sbjct: 1123 IEEPASPEPETAEVSIADIEEAFFEDPEEIPTIRLNMDAFTSNLKKIMEHNKELQDGNMS 1182

Query: 143  RALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLCILS 202
             ALV +    AS+PMPKLK+I +LRTEH+ YELPD HPLL +LE+REP+DPC YLL I +
Sbjct: 1183 SALVALTAETASLPMPKLKNISQLRTEHRVYELPDEHPLLAQLEKREPDDPCSYLLAIWT 1242

Query: 203  PGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMRGNFP 262
            PGET +S +P  + C ++  G LC E +C SCN+++E  S +VRGTILIPCRTAMRG+FP
Sbjct: 1243 PGETADSIQPSVSTCIFQANGMLCDEETCFSCNSIKETRSQIVRGTILIPCRTAMRGSFP 1302

Query: 263  LNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQYCFQ 315
            LNGTYFQVNEVFAD  SS NPI+VPRE IW+LPRR  YFGTS  TIF+GL+ E IQ CF 
Sbjct: 1303 LNGTYFQVNEVFADHASSLNPINVPRELIWELPRRTVYFGTSVPTIFKGLSTEKIQACFW 1357

BLAST of Moc02g02750 vs. TAIR 10
Match: AT3G10010.1 (demeter-like 2 )

HSP 1 Score: 245.7 bits (626), Expect = 5.3e-65
Identity = 147/306 (48.04%), Postives = 185/306 (60.46%), Query Frame = 0

Query: 23   SARLALPGLAEKRMVSSMVPDKSFEGTAQVMNPESILQIDGNPSSESRCQSNNREPIIEE 82
            SARLALP   E    S M+ ++  +    V+N    L +      E++ +S N EPIIEE
Sbjct: 995  SARLALPEPEESDRTSVMIHERRSKRKPVVVNFRPSLFLYQEKEQEAQ-RSQNCEPIIEE 1054

Query: 83   PQSPEPAYDESQLTDI----------EDLCEYDSDDVPTIRLSSGRFTTTSQNCM---ED 142
            P SPEP Y E  + D           ED  E + D +PTI L+  +   TS + +   E 
Sbjct: 1055 PASPEPEYIEHDIEDYPRDKNNVGTSEDPWE-NKDVIPTIILN--KEAGTSHDLVVNKEA 1114

Query: 143  NTTRALVPVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFELERREPNDPCPYLLC 202
             T+  LV + T  A+IP  KLK  ++LRTEH  +ELPD H +L   ERRE  D  PYLL 
Sbjct: 1115 GTSHDLVVLSTYAAAIPRRKLKIKEKLRTEHHVFELPDHHSILEGFERREAEDIVPYLLA 1174

Query: 203  ILSPGETINSSEPPNTRCT-YKELGELCSEGSCSSCNTVRERNSGMVRGTILIPCRTAMR 262
            I +PGET+NS +PP  RC  ++    LC+E  C  CN  RE  S  VRGTILIPCRTAMR
Sbjct: 1175 IWTPGETVNSIQPPKQRCALFESNNTLCNENKCFQCNKTREEESQTVRGTILIPCRTAMR 1234

Query: 263  GNFPLNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTSTTTIFRGLAKEDIQ 315
            G FPLNGTYFQ NEVFAD +SS NPI VP E IWDL RR+ Y G+S ++I +GL+ E I+
Sbjct: 1235 GGFPLNGTYFQTNEVFADHDSSINPIDVPTELIWDLKRRVAYLGSSVSSICKGLSVEAIK 1294

BLAST of Moc02g02750 vs. TAIR 10
Match: AT4G34060.1 (demeter-like protein 3 )

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-48
Identity = 114/259 (44.02%), Postives = 152/259 (58.69%), Query Frame = 0

Query: 77   EPIIEEPQSPEPAYDESQLTDIEDL--------------CEYDSD----DVPTIRLSSGR 136
            +P++E P SP     ES  TDIED+               ++D D     V    + SGR
Sbjct: 756  KPLVEFPSSPRAEIPES--TDIEDVPFMNLYQSYASVPKIDFDLDALKKSVEDALVISGR 815

Query: 137  FTTTSQNCMEDNTTRALV---PVHTRVASIPMPKLKHIDRLRTEHQAYELPDTHPLLFEL 196
             +++     ++  ++ALV   P +  +   P  K+K+ +RLRTEH  Y LPD H LL + 
Sbjct: 816  MSSS-----DEEISKALVIPTPENACIPIKPPRKMKYYNRLRTEHVVYVLPDNHELLHDF 875

Query: 197  ERREPNDPCPYLLCILSPGETINSSEPPNTRCTYKELGELCSEGSCSSCNTVRERNSGMV 256
            ERR+ +DP PYLL I  PGET +S  PP  +C+  +  +LC   +CS C T+RE+NS + 
Sbjct: 876  ERRKLDDPSPYLLAIWQPGETSSSFVPPKKKCS-SDGSKLCKIKNCSYCWTIREQNSNIF 935

Query: 257  RGTILIPCRTAMRGNFPLNGTYFQVNEVFADDESSKNPIHVPREWIWDLPRRITYFGTST 315
            RGTILIPCRTAMRG FPLNGTYFQ NEVFAD E+S NPI   RE    L +R  Y G++ 
Sbjct: 936  RGTILIPCRTAMRGAFPLNGTYFQTNEVFADHETSLNPIVFRRELCKGLEKRALYCGSTV 995

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016902242.16.6e-13478.15PREDICTED: protein ROS1-like isoform X2 [Cucumis melo][more]
XP_008458622.16.6e-13478.15PREDICTED: protein ROS1-like isoform X1 [Cucumis melo] >KAA0033377.1 protein ROS... [more]
XP_022958534.16.2e-13278.48protein ROS1-like [Cucurbita moschata][more]
XP_023532993.11.4e-13178.48protein ROS1-like [Cucurbita pepo subsp. pepo][more]
XP_022996087.19.8e-13076.82protein ROS1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
C7IW648.5e-8452.96Protein ROS1A OS=Oryza sativa subsp. japonica OX=39947 GN=ROS1A PE=1 SV=2[more]
Q8LK561.8e-8153.97Transcriptional activator DEMETER OS=Arabidopsis thaliana OX=3702 GN=DME PE=1 SV... [more]
Q9SJQ63.1e-7853.64DNA glycosylase/AP lyase ROS1 OS=Arabidopsis thaliana OX=3702 GN=ROS1 PE=1 SV=2[more]
B8YIE82.0e-7248.84Protein ROS1C OS=Oryza sativa subsp. japonica OX=39947 GN=ROS1C PE=2 SV=2[more]
Q9SR667.5e-6448.04DEMETER-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=DML2 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7SVP43.2e-13478.15Protein ROS1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S4E1Z03.2e-13478.15protein ROS1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497972 PE=3 SV=1[more]
A0A1S3C8A03.2e-13478.15protein ROS1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497972 PE=3 SV=1[more]
A0A6J1H5D53.0e-13278.48protein ROS1-like OS=Cucurbita moschata OX=3662 GN=LOC111459738 PE=3 SV=1[more]
A0A6J1K0Y64.8e-13076.82protein ROS1-like OS=Cucurbita maxima OX=3661 GN=LOC111491404 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G04560.11.3e-8253.97HhH-GPD base excision DNA repair family protein [more]
AT5G04560.21.3e-8253.97HhH-GPD base excision DNA repair family protein [more]
AT2G36490.12.2e-7953.64demeter-like 1 [more]
AT3G10010.15.3e-6548.04demeter-like 2 [more]
AT4G34060.12.0e-4844.02demeter-like protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR028924Permuted single zf-CXXC unitPFAMPF15629Perm-CXXCcoord: 203..232
e-value: 7.1E-8
score: 32.6
IPR028925Demeter, RRM-fold domainPFAMPF15628RRM_DMEcoord: 237..315
e-value: 3.2E-39
score: 133.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..91
IPR044811DNA glycosylase, plantPANTHERPTHR46213TRANSCRIPTIONAL ACTIVATOR DEMETERcoord: 23..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g02750.1Moc02g02750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006287 base-excision repair, gap-filling
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0080111 DNA demethylation
biological_process GO:0045004 DNA replication proofreading
biological_process GO:0006272 leading strand elongation
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006297 nucleotide-excision repair, DNA gap filling
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0008622 epsilon DNA polymerase complex
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0035514 DNA demethylase activity
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0019104 DNA N-glycosylase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0008310 single-stranded DNA 3'-5' exodeoxyribonuclease activity