Moc02g02080 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g02080
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cysteine desulfurase
Location	chr2: 1542707 .. 1546985 (-)
RNA-Seq Expression	Moc02g02080
Synteny	Moc02g02080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTAGTAGCATCGATCGACACCAGGCTTGCTCGTGCTCGATTCTCAAGTTGGGGAACGAGAGGATGATGGAAGTTGTGGTTCTGAAGCTCCCATCCTTCCAATTCATAAACCCTAGTCGCTGCAGTTCCTGTTTCTCCTCCACTCTCCGCAACTCTCCTTCCTCCGCACTCCGCAGATTTTCCTCTCCTTTCTCAGTTTCCGCTTCTGCTTCCACCGCCACGGAAACCGACAGCTTACATTTCGCCTCTCTTTCGCTTGGCCACACAACCCGCCCCGATTTCCCCATCCTCCATCAGGTTCCTGTTTTTTGAGTTCTTTATGTTAATCTCTGGTCATCGCAAGGCCAATTTTTCGGTCTATTTGTTATTGGATCCTTCAAGCTTGGTTGAATCGTCAGTTTTTCCCGAGTATATTGTGAATCACGTTGTATTGAGTATTGATGTCTCTAAATATTGTTATTGTTATTGTATTGTATTGGCTCTTCATGCGGTGGAGTTAATTGCCGTGTTGCGACCTGCAGGAAGTAAATGGCTCTAAGCTTGTATACTTGGACAACGCTGCTACCTCTCAGAAGCCCATTGCTGTGTTGAAAGCTTTGCAGAATTATTATGAGGCTTATAATTCAAATGTTCATCGTGGAATACACTACTTGAGGTAGATAATCTACACTTCGGGGTTAAATTAATTAATTATAGTATTACTTGCTGGGTTATAGGCACTAAATTAGTGTCTGTCTGCATTTATCACTTGTAATCACGACCGTTTCTGACTGTAGTGCGAAGGCAACTGATGAGTATGAGCTGGCAAGAAAAAAGGTTGCTTCTTTTGTCAATGCAGCAGAAGCAAGAGAGATTATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCTAATTTAAAATCAGAGGATGAGGTGCGTATCATGTTAGGTTGGGTTACTGTATCAATGTTAGTTGTTTAAGGCAAGTGATATTTCTTGTAAATATGTGCTATCTAGTGCACGTATAGGTACTTGTAACATGTATTTGTTAATGTTATTCTCCACACGGGAGATTTAATCTGAAACGCAGTTTGTTTCTGATAATTATTTATGCAGAAAAAAAGTATGCTCTATCCACAAATACCTGGGATTTTTTTAGTAGTTTTGCAAACTTGACTATTGTAAATTGTTTCTTCAGTCTTTGTATCTTCAGTTGGTGCAGAATTTATATGCCGTGGATGGTCATATTGTTTGAGTTTCATGAAGTGGTATTTGTAGAAGTTTTGTCATTTCCATCTCAAATCTATCAATTTAAATTGGTTTACATTTTATACTTGTTCATATTCTTTTCAGTTGTCTTAAGTGGGGTTTTTGTAAAGAGAGGAAAAACTGAGTTTTAGGCTATGGTTCATTGGGATTATCTGCACTTGAATATAGTTTGATTTTTGTGACTGGTCTGTTTGTGAAGATGCTTGTGATGTCAAATTTAAGTTAATAAGAATGTTTTTAACTACTACATGAAGACCATAAAAGCTGCATTTATTATTTTTAAGTGTGTGCCTTGTACTGCGTTAACTTTAACTGTAACGATGTTTTTCCTTCAGTGCAGATCATACTTACGGTTGCTGAACATCATAGTGCAATTGTCCCCTGGCAACTTGTGGCTGAAAGGACGGGTGCTGTTCTGAAGTTTGTGAGTTTGGATGAACATGATGTTCCGAAATTGGCAGATTTAAAGGAGATGTTTACAACAAAAACGAAGCTTGTGGTTACCCATCATGTTTCAAATGTACTAGGTACACTCTTCTATCTGATATTTTAGGATTATTTTAGTCAATTTCCTTTTCTCCCCCATTTTATTAGTAAACATCACTCAAGTGAGATAAGTACCTGCTACTAGGTGAATTTTTCAAACTTAAGTGTGATCATATCACTTTTATTATTACGTGTCAAGTGGAAAGTTGTATGGTTGAATAATAGTAATAAAGTTCAATGAATGGGAAAAGAAAGAGAAGAACTGCATGACATACCCCCTTCTCTTGATTTCTGTCTCTTATTCTCGATGCCCTTCTAATAGTAACTCTAGTCGAATTCGATATGATTTGATTCTTTCTGCAGCATCTGTTCTTCCAATTGAAGATATAGTTGGCTGGGCACATCGTTTTGGGGCAAAAGTGCTAGTAGATGCATGCCAGAGTGTTCCACATATGGTTGTTGACGTCCAGGCACTTGACGCTGACTTTCTTGTTGCTTCTTCTCACAAGGTTAGGCCATTCTCTTATTAATAAAATCTTGGGCCTTACATTGTGCGTATGAAGTTAAGGAATAAACATGCTTCAGATGTGTGGGCCTACAGGCATCGGATTTTTATATGGAAAGAGTGATCTCTTGTCTGCAATGCCTCCATTCTTAGGTAGGTTCTTTGAAAAGATAAGTTATGAAAGTAACTGTATATAAAAACAATTTTACCAGTTAGTTAATGAGAAGTCAATATCCAGTGTTCTAATTTGGTCCTACATGGCTCTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGATCATTCCACATATGCAGAACCTCCTTCAAGGTTGGTGAACTGCAAGCGTGCAATTTTGTATTTTTCTTTGTATTTTAACTTAACCATAATTTTCTCCATGTATCTAAATGGGGTGGAAAATTTTGTACATTCGAGATATAGGACTTTGAATGCTGAATATGTCATGAGTTTCCATGTTTTATTTATTGAGATTAGAACACCAAAAGTTCGTCTCTTTTTTCTTCTTTGACCTATGGTTATGTGGAAGCCGGAACTTTAGTGTTTTGCTCATTAAGTTTCTATGCTGGCTACTGTGTGTGTTTACGCTTGCTTCTTGTATGCACTTTTCGTTTGCTCATCTGTTGTGTTCGTAGCAATTGTGACACTTGGCTTAATTGTGTACTTGATAAATCATATATTTGAAGTCATCATGAGAATTGAGATCTAAACAACTGACTTCATGAACGAATGGAGTTAAAATTCTGTGGTGTCTATTTATTACAGTATTCATTAATTGATGTCATTTTACTTATCTTTTTATGTCTTTTATGTTGCCTTCTTTTGATATTTGCATCAGAAAGTTATTTTGGTTGGACAAACTTCCGACTCTAGTCCCTACTAATAACTCATTTTTCCCTTGATTAGATTTGAGGCTGGGACGCCAGCAATTGGAGAAGCAATTGGGTTGGGAGCTGCTGTTGACTATTTGTCTGGAATCGGCATGCAAAAGATACATAGTTATGAAGTGAGTTGATTGCATTTTGGCATATGCATTCCCCTTCCTTTAAGAAATTTATTGAGGTGAAAAGGTTGGTGTCAATACTAAATATAGCAGCACACAACACAGCAAAGGAACATTTTCCTCAATCAAAACATTTAAATATTATTTTTCATCAACTAGGTTACCTGGAAATGTGGAATTGAAAACTGAAAATTTGAATGAGATGATAAACATTTCTACGAGCTTTTATGTTCAAGTCTAACGGTAAGGAGTTGGTGTCTGCAAACAGGAAGAATTGGCAAGATATCTATATGAAAGCCTTCGTGCAGTCCCCAATATTCGCATTTACGGACCAGCTCCCACAGCAAATGTGCAACGTGCTGCTCTGTGTTCTTTCAATGTTGAGAATATTCATCCAACGGACATTGCAACTTTTCTTGACCAACAGGTGATACATCTCTGACTTGGCTCTACTTTGTAGGACATGCTTGAGTTTGTTCCCGACCAAAGTTTTTTAAGGTTTAAATTCCTATCCCTAAAAAAAAAAAAAAGTTTCGTGATTATTCTAATTTGGTCTCTAGAGAAGATATCTACGTTGACAAACAAGTATTATTGGTAGGTCAAGTGTCGAGTTCTAAAATAGACTTTAGAGATTCTAAAACCAAAGAGTTGTAAGTCAAAGGGACCAAAACATAGTATCTAAAATTTTAGGGACTAGAATGAAATACCAAGAACCAGAATAGGGTCTAAAATCTCTTCTCTAACCATGTTAAAGTCTCAAGCTCTTAAAACAGTATCATGATAAATTATGTTTATGGATCCTTAAAATATTGCAATGCAGCATGGTGTCGCTATCAGATCGGGACACCACTGTGCGCAGCCCCTCCACCGTGCTCTAGGGGTCAGCTCGAGCGCCCGAGCTAGCCTATACTTCTACAACACGAAAGAGGATGTCGACTACTTTATCCAGGCTCTAAATGACACTGTAAGTTTCTTCAACTCTTTTAAGTAG

mRNA sequence

ATGATTAGTAGCATCGATCGACACCAGGCTTGCTCGTGCTCGATTCTCAAGTTGGGGAACGAGAGGATGATGGAAGTTGTGGTTCTGAAGCTCCCATCCTTCCAATTCATAAACCCTAGTCGCTGCAGTTCCTGTTTCTCCTCCACTCTCCGCAACTCTCCTTCCTCCGCACTCCGCAGATTTTCCTCTCCTTTCTCAGTTTCCGCTTCTGCTTCCACCGCCACGGAAACCGACAGCTTACATTTCGCCTCTCTTTCGCTTGGCCACACAACCCGCCCCGATTTCCCCATCCTCCATCAGGAAGTAAATGGCTCTAAGCTTGTATACTTGGACAACGCTGCTACCTCTCAGAAGCCCATTGCTGTGTTGAAAGCTTTGCAGAATTATTATGAGGCTTATAATTCAAATGTTCATCGTGGAATACACTACTTGAGTGCGAAGGCAACTGATGAGTATGAGCTGGCAAGAAAAAAGGTTGCTTCTTTTGTCAATGCAGCAGAAGCAAGAGAGATTATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCTAATTTAAAATCAGAGGATGAGATCATACTTACGGTTGCTGAACATCATAGTGCAATTGTCCCCTGGCAACTTGTGGCTGAAAGGACGGGTGCTGTTCTGAAGTTTGTGAGTTTGGATGAACATGATGTTCCGAAATTGGCAGATTTAAAGGAGATGTTTACAACAAAAACGAAGCTTGTGGTTACCCATCATGTTTCAAATGTACTAGCATCTGTTCTTCCAATTGAAGATATAGTTGGCTGGGCACATCGTTTTGGGGCAAAAGTGCTAGTAGATGCATGCCAGAGTGTTCCACATATGGTTGTTGACGTCCAGGCACTTGACGCTGACTTTCTTGTTGCTTCTTCTCACAAGATGTGTGGGCCTACAGGCATCGGATTTTTATATGGAAAGAGTGATCTCTTGTCTGCAATGCCTCCATTCTTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGATCATTCCACATATGCAGAACCTCCTTCAAGATTTGAGGCTGGGACGCCAGCAATTGGAGAAGCAATTGGGTTGGGAGCTGCTGTTGACTATTTGTCTGGAATCGGCATGCAAAAGATACATAGTTATGAAGAAGAATTGGCAAGATATCTATATGAAAGCCTTCGTGCAGTCCCCAATATTCGCATTTACGGACCAGCTCCCACAGCAAATGTGCAACGTGCTGCTCTGTGTTCTTTCAATGTTGAGAATATTCATCCAACGGACATTGCAACTTTTCTTGACCAACAGCATGGTGTCGCTATCAGATCGGGACACCACTGTGCGCAGCCCCTCCACCGTGCTCTAGGGGTCAGCTCGAGCGCCCGAGCTAGCCTATACTTCTACAACACGAAAGAGGATGTCGACTACTTTATCCAGGCTCTAAATGACACTGTAAGTTTCTTCAACTCTTTTAAGTAG

Coding sequence (CDS)

ATGATTAGTAGCATCGATCGACACCAGGCTTGCTCGTGCTCGATTCTCAAGTTGGGGAACGAGAGGATGATGGAAGTTGTGGTTCTGAAGCTCCCATCCTTCCAATTCATAAACCCTAGTCGCTGCAGTTCCTGTTTCTCCTCCACTCTCCGCAACTCTCCTTCCTCCGCACTCCGCAGATTTTCCTCTCCTTTCTCAGTTTCCGCTTCTGCTTCCACCGCCACGGAAACCGACAGCTTACATTTCGCCTCTCTTTCGCTTGGCCACACAACCCGCCCCGATTTCCCCATCCTCCATCAGGAAGTAAATGGCTCTAAGCTTGTATACTTGGACAACGCTGCTACCTCTCAGAAGCCCATTGCTGTGTTGAAAGCTTTGCAGAATTATTATGAGGCTTATAATTCAAATGTTCATCGTGGAATACACTACTTGAGTGCGAAGGCAACTGATGAGTATGAGCTGGCAAGAAAAAAGGTTGCTTCTTTTGTCAATGCAGCAGAAGCAAGAGAGATTATTTTTACCAGGAATGCAACTGAAGCTATCAATCTAGTGGCTTATTCTTGGGGCCTAGCTAATTTAAAATCAGAGGATGAGATCATACTTACGGTTGCTGAACATCATAGTGCAATTGTCCCCTGGCAACTTGTGGCTGAAAGGACGGGTGCTGTTCTGAAGTTTGTGAGTTTGGATGAACATGATGTTCCGAAATTGGCAGATTTAAAGGAGATGTTTACAACAAAAACGAAGCTTGTGGTTACCCATCATGTTTCAAATGTACTAGCATCTGTTCTTCCAATTGAAGATATAGTTGGCTGGGCACATCGTTTTGGGGCAAAAGTGCTAGTAGATGCATGCCAGAGTGTTCCACATATGGTTGTTGACGTCCAGGCACTTGACGCTGACTTTCTTGTTGCTTCTTCTCACAAGATGTGTGGGCCTACAGGCATCGGATTTTTATATGGAAAGAGTGATCTCTTGTCTGCAATGCCTCCATTCTTAGGTGGTGGAGAGATGATCTCAGATGTATTTCTTGATCATTCCACATATGCAGAACCTCCTTCAAGATTTGAGGCTGGGACGCCAGCAATTGGAGAAGCAATTGGGTTGGGAGCTGCTGTTGACTATTTGTCTGGAATCGGCATGCAAAAGATACATAGTTATGAAGAAGAATTGGCAAGATATCTATATGAAAGCCTTCGTGCAGTCCCCAATATTCGCATTTACGGACCAGCTCCCACAGCAAATGTGCAACGTGCTGCTCTGTGTTCTTTCAATGTTGAGAATATTCATCCAACGGACATTGCAACTTTTCTTGACCAACAGCATGGTGTCGCTATCAGATCGGGACACCACTGTGCGCAGCCCCTCCACCGTGCTCTAGGGGTCAGCTCGAGCGCCCGAGCTAGCCTATACTTCTACAACACGAAAGAGGATGTCGACTACTTTATCCAGGCTCTAAATGACACTGTAAGTTTCTTCAACTCTTTTAAGTAG

Protein sequence

MISSIDRHQACSCSILKLGNERMMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK

Homology

[Close]

BLAST of Moc02g02080 vs. NCBI nr
Match: XP_022153071.1 (cysteine desulfurase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 937.2 bits (2421), Expect = 5.9e-269
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 82
           MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF
Sbjct: 1   MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 60

Query: 83  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 142
           ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH
Sbjct: 61  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 120

Query: 143 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 202
           YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT
Sbjct: 121 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 180

Query: 203 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 262
           VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS
Sbjct: 181 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 240

Query: 263 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 322
           VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK
Sbjct: 241 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 300

Query: 323 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 382
           SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ
Sbjct: 301 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 360

Query: 383 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 442
           KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH
Sbjct: 361 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 420

Query: 443 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 498
           GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK
Sbjct: 421 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 475

[Close]

BLAST of Moc02g02080 vs. NCBI nr
Match: XP_023513282.1 (cysteine desulfurase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 868.2 bits (2242), Expect = 3.4e-248
Identity = 441/483 (91.30%), Postives = 457/483 (94.62%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQ----FINPSRCSSCFSSTLRNSPSSAL----RRFSSPFSVSASASTA 82
           MME VVLKLPSFQ    FINPSRCSSCFSSTLR S S +L    RRFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQLLFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 83  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 142
            ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 143 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 202
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 203 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 262
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTKTKLVVTH 240

Query: 263 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 322
           HVSNVLASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 323 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 382
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 383 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 442
           YLSGIGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N +RAALCSFN+EN+HPTDI
Sbjct: 361 YLSGIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAERAALCSFNIENMHPTDI 420

Query: 443 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 498
           ATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFD 480

[Close]

BLAST of Moc02g02080 vs. NCBI nr
Match: KAG6570453.1 (Protein DGS1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 865.5 bits (2235), Expect = 2.2e-247
Identity = 443/494 (89.68%), Postives = 459/494 (92.91%), Query Frame = 0


Query: 16   LKLGNERMMEVVVLKLPSFQ----FINPSRCSSCFSSTLRNSPSSAL----RRFSSPFSV 75
            LK GNERMME VVLKLPSFQ    FINPSRCSSCFSSTLR S S +L    RRFSSPFSV
Sbjct: 606  LKFGNERMMEGVVLKLPSFQFQLHFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSV 665

Query: 76   SASASTATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQ 135
            SASASTA ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQ
Sbjct: 666  SASASTARETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQ 725

Query: 136  NYYEAYNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYS 195
            NYYEAYNSNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYS
Sbjct: 726  NYYEAYNSNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYS 785

Query: 196  WGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTK 255
            WG+ANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTK
Sbjct: 786  WGIANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTK 845

Query: 256  TKLVVTHHVSNV-----LASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADF 315
            TKLVVTHHVSN       ASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADF
Sbjct: 846  TKLVVTHHVSNYDLIVSAASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADF 905

Query: 316  LVASSHKMCGPTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPA 375
            LVASSHKMCGPTGIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPA
Sbjct: 906  LVASSHKMCGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPA 965

Query: 376  IGEAIGLGAAVDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALC 435
            IGEAIGLGAA+DYLS IGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALC
Sbjct: 966  IGEAIGLGAAIDYLSEIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALC 1025

Query: 436  SFNVENIHPTDIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYF 495
            SFN+EN+HPTDIATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYF
Sbjct: 1026 SFNIENMHPTDIATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYF 1085

Query: 496  IQALNDTVSFFNSF 497
            IQALNDTV+FF+SF
Sbjct: 1086 IQALNDTVNFFDSF 1097

[Close]

BLAST of Moc02g02080 vs. NCBI nr
Match: XP_022943969.1 (cysteine desulfurase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 865.1 bits (2234), Expect = 2.8e-247
Identity = 440/483 (91.10%), Postives = 456/483 (94.41%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQ----FINPSRCSSCFSSTLRNSPSSAL----RRFSSPFSVSASASTA 82
           MME VVLKLPSFQ    FINPSRCSSCFSSTLR S S +L    RRFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQLHFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 83  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 142
            ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 143 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 202
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 203 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 262
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTKTKLVVTH 240

Query: 263 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 322
           HVSNVLASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 323 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 382
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 383 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 442
           YLS IGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN+EN+HPTDI
Sbjct: 361 YLSEIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIENMHPTDI 420

Query: 443 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 498
           ATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTV+FF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVNFFD 480

[Close]

BLAST of Moc02g02080 vs. NCBI nr
Match: XP_022986219.1 (cysteine desulfurase 1, chloroplastic-like, partial [Cucurbita maxima])

HSP 1 Score: 857.1 bits (2213), Expect = 7.7e-245
Identity = 435/480 (90.62%), Postives = 451/480 (93.96%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQ----FINPSRCSSCFSSTLRNSPSSAL----RRFSSPFSVSASASTA 82
           MME VVLKLPSFQ    F+NPSRCSSCFSSTLR S S +L    RRFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQFHFMNPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 83  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 142
            ETD+LH  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 143 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 202
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 203 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 262
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEM TTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMLTTKTKLVVTH 240

Query: 263 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 322
           HVSNVLASVLPIEDIV WAH FGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHHFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 323 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 382
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 383 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 442
           YLSGIGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN++N+HPTDI
Sbjct: 361 YLSGIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIDNMHPTDI 420

Query: 443 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 495
           ATFLDQQHG+AIRSGHHCAQPLHR LGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRVLGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFD 478

[Close]

BLAST of Moc02g02080 vs. ExPASy Swiss-Prot
Match: Q93WX6 (Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NFS2 PE=1 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 7.9e-200
Identity = 356/474 (75.11%), Postives = 400/474 (84.39%), Query Frame = 0


Query: 24  MEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHFA 83
           ME V +KLPSF    P+  S    S  R        R SS  SV ++A+ ++ T S    
Sbjct: 1   MEGVAMKLPSF----PNAISIGHRSFSR-------VRCSSSLSVCSAAAASSATISTDSE 60

Query: 84  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHY 143
           S+SLGH  R DF ILHQEVNGSKLVYLD+AATSQKP AVL ALQNYYE YNSNVHRGIHY
Sbjct: 61  SVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHY 120

Query: 144 LSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTV 203
           LSAKATDE+ELARKKVA F+NA+++REI+FTRNATEAINLVAYSWGL+NLK  DE+ILTV
Sbjct: 121 LSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTV 180

Query: 204 AEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASV 263
           AEHHS IVPWQ+V+++TGAVLKFV+L+E +VP +  L+E+ + KTKLV  HHVSNVLAS 
Sbjct: 181 AEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASS 240

Query: 264 LPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKS 323
           LPIE+IV WAH  GAKVLVDACQSVPHMVVDVQ L+ADFLVASSHKMCGPTGIGFLYGKS
Sbjct: 241 LPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKS 300

Query: 324 DLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQK 383
           DLL +MPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAI LGAAVDYLSGIGM K
Sbjct: 301 DLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPK 360

Query: 384 IHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHG 443
           IH YE E+ +YLYE L ++P++RIYGP P+ +V R ALCSFNVE +HPTD+ATFLDQQHG
Sbjct: 361 IHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQHG 420

Query: 444 VAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 498
           VAIRSGHHCAQPLHR LGV++SARASLYFYNTK+DVD FI AL DTVSFFNSFK
Sbjct: 421 VAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFFNSFK 463

[Close]

BLAST of Moc02g02080 vs. ExPASy Swiss-Prot
Match: Q55793 (Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=csd PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.8e-148
Identity = 260/409 (63.57%), Postives = 309/409 (75.55%), Query Frame = 0


Query: 86  SLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLS 145
           SL  T R DFPIL+QE+NG  LVYLDNAATSQKP AVL+ L +YYE  N+NVHRG H LS
Sbjct: 8   SLAATVRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDNANVHRGAHQLS 67

Query: 146 AKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAE 205
            +ATD YE  R KVA F+NA   REI++TRNATEAINLVAYSWG+ NLK+ DEII TV E
Sbjct: 68  VRATDAYEAVRNKVAKFINARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVME 127

Query: 206 HHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLP 265
           HHS +VPWQ+VA +TGAVLKFV LDE +   L   K + + KTKLV   H+SN L  V P
Sbjct: 128 HHSNLVPWQMVAAKTGAVLKFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNP 187

Query: 266 IEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDL 325
            E+I   AH+ GAKVLVDACQS PH  +DVQ +D D+LVAS HKMC PTGIGFLYGK ++
Sbjct: 188 AEEIAQLAHQAGAKVLVDACQSAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEI 247

Query: 326 LSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIH 385
           L AMPPF GGGEMI++VF DH T  E P +FEAGTPAI EAI LGAAVDYL+ +GM+ IH
Sbjct: 248 LEAMPPFFGGGEMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLTDLGMENIH 307

Query: 386 SYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVA 445
           +YE EL  YL++ L  +P +R+YGP P  +  RAAL SFNV  +H +D+AT +DQ  G+A
Sbjct: 308 NYEVELTHYLWQGLGQIPQLRLYGPNP-KHGDRAALASFNVAGLHASDVATMVDQD-GIA 367

Query: 446 IRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 495
           IRSGHHC QPLHR    S SARASLYFYNTKE++D F+Q+L  T+ FF+
Sbjct: 368 IRSGHHCTQPLHRLFDASGSARASLYFYNTKEEIDLFLQSLQATIRFFS 414

[Close]

BLAST of Moc02g02080 vs. ExPASy Swiss-Prot
Match: O32164 (Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) OX=224308 GN=sufS PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 3.4e-118
Identity = 211/404 (52.23%), Postives = 277/404 (68.56%), Query Frame = 0


Query: 92  RPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDE 151
           R  FPILHQ+VNG  LVYLD+AATSQKP AV++ L  YY  YNSNVHRG+H L  +ATD 
Sbjct: 7   REQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDG 66

Query: 152 YELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIV 211
           YE AR+KV  F+NA    EIIFT+  T ++N+VA S+  ANLK  DE+++T  EHH+ I+
Sbjct: 67  YEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANII 126

Query: 212 PWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVG 271
           PWQ   + TGA LK++ L E     L D++E  T+ TK+V   HVSNVL +V PI+++  
Sbjct: 127 PWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAK 186

Query: 272 WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPP 331
            AH  GA ++VD  QS PHM +DVQ LD DF   SSHKMCGPTG+G LYGK  LL  M P
Sbjct: 187 IAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKALLENMEP 246

Query: 332 FLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEEL 391
              GGEMI  V L  ST+ E P +FEAGTP I  AIGLGAA+D+L  IG+ +I  +E +L
Sbjct: 247 AEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKL 306

Query: 392 ARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHH 451
           A Y  E  R +  + +YGP      +RA L +FN++++HP D+AT LD + G+A+R+GHH
Sbjct: 307 AAYALERFRQLDGVTVYGPE-----ERAGLVTFNLDDVHPHDVATVLDAE-GIAVRAGHH 366

Query: 452 CAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNS 496
           CAQPL + L V+++ARAS Y YNT+E++D  ++AL  T  +F +
Sbjct: 367 CAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKEYFTN 404

[Close]

BLAST of Moc02g02080 vs. ExPASy Swiss-Prot
Match: Q9K7A0 (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=csd PE=3 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 8.3e-117
Identity = 215/405 (53.09%), Postives = 276/405 (68.15%), Query Frame = 0


Query: 89  HTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKA 148
           H  +  FP+L QEVNGS LVYLD+AATSQKPIAV++AL +YY  YNSNVHRG+H L   A
Sbjct: 4   HEIKKLFPVLDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRRYNSNVHRGVHTLGTLA 63

Query: 149 TDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHS 208
           TD YE AR+K+  F++A+   EIIFTR  T AINLVA S+G ANL   DEI++T  EHHS
Sbjct: 64  TDGYEGAREKIRRFIHASSTEEIIFTRGTTTAINLVAASYGRANLGEGDEIVITPMEHHS 123

Query: 209 AIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIED 268
            I+PWQ VA+ TGA L ++ L +    K+ D+++  + KTK+V   HVSNVL ++ P+++
Sbjct: 124 NIIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTKIVAIMHVSNVLGTINPVKE 183

Query: 269 IVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSA 328
           I   AHR GA +LVD  QS PHM +DVQ L  DF   S HKM GPTGIG LYGK   L  
Sbjct: 184 IAEIAHRHGAIMLVDGAQSAPHMKIDVQELGCDFFAFSGHKMAGPTGIGVLYGKKAHLEK 243

Query: 329 MPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYE 388
           M P   GGEMI  V L  ST+ E P +FE GTP I  AIGLGAA+D+L  IG+ +I  +E
Sbjct: 244 MEPVEFGGEMIDFVGLYDSTWKELPWKFEGGTPIIAGAIGLGAAIDFLEDIGLDEIEKHE 303

Query: 389 EELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRS 448
            ELA+Y  + L  +  + +YGP      +RA L +FN+E++HP D+AT LD   G+A+R+
Sbjct: 304 HELAQYALDRLSELEGMTVYGPQ-----KRAGLVTFNIEDVHPHDVATVLDAD-GIAVRA 363

Query: 449 GHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF 494
           GHHCAQPL + L V+++ARAS Y YNTKED+D   + L  T  +F
Sbjct: 364 GHHCAQPLMKWLDVTATARASFYLYNTKEDIDALAKGLEKTKEYF 402

[Close]

BLAST of Moc02g02080 vs. ExPASy Swiss-Prot
Match: Q8CTA4 (Probable cysteine desulfurase OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=csd PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.1e-113
Identity = 207/401 (51.62%), Postives = 270/401 (67.33%), Query Frame = 0


Query: 94  DFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDEYE 153
           DFPIL Q+VN  +L YLD+ ATSQ P+ VL  L +YY+ YNSNVHRG+H L + ATD YE
Sbjct: 14  DFPILEQKVNNKRLAYLDSTATSQTPVQVLNVLDDYYKRYNSNVHRGVHTLGSLATDGYE 73

Query: 154 LARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPW 213
            AR+ V  F+NA    EIIFTR  T +IN+VA+S+G AN+   DEI++T  EHH+ IVPW
Sbjct: 74  NARETVRRFINAKYFEEIIFTRGTTASINIVAHSYGDANISEGDEIVVTEMEHHANIVPW 133

Query: 214 QLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVGWA 273
           Q +A+R  A LKF+ + +    +L D+K     KTK+V   HVSNVL ++  ++ I   A
Sbjct: 134 QQLAKRKNATLKFIPMTKDGELQLDDIKATINDKTKIVAIAHVSNVLGTINDVKTIAKIA 193

Query: 274 HRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPPFL 333
           H  GA + VD  QS PHM +D+Q +DADF   S HKM GPTGIG LYGK +LL  M P  
Sbjct: 194 HEHGAVISVDGAQSAPHMALDMQDIDADFYSFSGHKMLGPTGIGVLYGKRELLQNMEPVE 253

Query: 334 GGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEELAR 393
            GG+MI  V    ST+A+ P++FEAGTP I +AIGL  A+ Y+  +G   IH +E+EL  
Sbjct: 254 FGGDMIDFVSKYDSTWADLPTKFEAGTPLIAQAIGLAEAIHYIENLGFNAIHQHEKELTE 313

Query: 394 YLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHHCA 453
           Y YE +  +  + IYGP      +RA + +FN+ +IHP D+AT +D + GVA+R+GHHCA
Sbjct: 314 YAYEQMLTIDGLEIYGPPKD---RRAGVITFNLADIHPHDVATAVDTE-GVAVRAGHHCA 373

Query: 454 QPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 495
           QPL + LGVSS+ARAS Y YNTKEDVD  +QAL  T  FF+
Sbjct: 374 QPLMKWLGVSSTARASFYVYNTKEDVDQLVQALKQTKEFFS 410

[Close]

BLAST of Moc02g02080 vs. ExPASy TrEMBL
Match: A0A6J1DJL0 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111020663 PE=4 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.8e-269
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 82
           MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF
Sbjct: 1   MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 60

Query: 83  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 142
           ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH
Sbjct: 61  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 120

Query: 143 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 202
           YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT
Sbjct: 121 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 180

Query: 203 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 262
           VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS
Sbjct: 181 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 240

Query: 263 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 322
           VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK
Sbjct: 241 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 300

Query: 323 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 382
           SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ
Sbjct: 301 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 360

Query: 383 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 442
           KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH
Sbjct: 361 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 420

Query: 443 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 498
           GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK
Sbjct: 421 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 475

[Close]

BLAST of Moc02g02080 vs. ExPASy TrEMBL
Match: A0A6J1FVQ2 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111448537 PE=4 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.4e-247
Identity = 440/483 (91.10%), Postives = 456/483 (94.41%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQ----FINPSRCSSCFSSTLRNSPSSAL----RRFSSPFSVSASASTA 82
           MME VVLKLPSFQ    FINPSRCSSCFSSTLR S S +L    RRFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQLHFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 83  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 142
            ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 143 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 202
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 203 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 262
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTKTKLVVTH 240

Query: 263 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 322
           HVSNVLASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 323 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 382
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 383 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 442
           YLS IGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN+EN+HPTDI
Sbjct: 361 YLSEIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIENMHPTDI 420

Query: 443 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 498
           ATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTV+FF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVNFFD 480

[Close]

BLAST of Moc02g02080 vs. ExPASy TrEMBL
Match: A0A6J1JFG5 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111484037 PE=4 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.7e-245
Identity = 435/480 (90.62%), Postives = 451/480 (93.96%), Query Frame = 0


Query: 23  MMEVVVLKLPSFQ----FINPSRCSSCFSSTLRNSPSSAL----RRFSSPFSVSASASTA 82
           MME VVLKLPSFQ    F+NPSRCSSCFSSTLR S S +L    RRFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQFHFMNPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 83  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 142
            ETD+LH  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 143 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 202
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 203 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 262
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEM TTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMLTTKTKLVVTH 240

Query: 263 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 322
           HVSNVLASVLPIEDIV WAH FGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHHFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 323 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 382
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 383 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 442
           YLSGIGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN++N+HPTDI
Sbjct: 361 YLSGIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIDNMHPTDI 420

Query: 443 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 495
           ATFLDQQHG+AIRSGHHCAQPLHR LGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRVLGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFD 478

[Close]

BLAST of Moc02g02080 vs. ExPASy TrEMBL
Match: A0A1S3BLT6 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103491447 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.8e-240
Identity = 430/485 (88.66%), Postives = 450/485 (92.78%), Query Frame = 0


Query: 23  MMEVVVLKLPS------FQFINPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASAS 82
           MME +VLKLPS      FQ +NP+RC SCFSSTLR S S  LR    RFSSP SVSASAS
Sbjct: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60

Query: 83  TATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEA 142
           TA ETDSLH  SLSLGHTTRPDFPILHQE+NGSKLVYLDNAATSQKPI+VL ALQNYYEA
Sbjct: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120

Query: 143 YNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLAN 202
           YNSNVHRGIH LSAKATDEYE ARKKVASF+NA +A+EIIFTRNATEAINLVAYSWGLAN
Sbjct: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180

Query: 203 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVV 262
           LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSL EHDVP L D KEMF+TKTKLVV
Sbjct: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240

Query: 263 THHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG 322
           THHVSNVLASVLPIE+IVG AHRFGAKVLVDACQSVPHMVV+VQALDADFLVASSHKMCG
Sbjct: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300

Query: 323 PTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 382
           PTGIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360

Query: 383 VDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPT 442
           +DYLSGIGM KIHSYEEELA YLYE+LRAVPNIRIYGPAPTA+VQRAALCSFN+ +IHPT
Sbjct: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420

Query: 443 DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSF 498
           DIATFLDQQHGVAIRSGHHCAQPLHRALGV+SSAR SLYFYNTKEDVDYFIQALNDTVSF
Sbjct: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480

[Close]

BLAST of Moc02g02080 vs. ExPASy TrEMBL
Match: A0A0A0KEG9 (Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G427980 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.1e-240
Identity = 429/487 (88.09%), Postives = 451/487 (92.61%), Query Frame = 0


Query: 21  ERMMEVVVLKLPS------FQFINPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSAS 80
           +RMME +VLK PS      FQF+NP+RC SCFSSTLR S S  LR    R  S FSVSAS
Sbjct: 28  QRMMEGMVLKSPSFHSQFHFQFMNPNRCISCFSSTLRASSSPLLRFRNPRLPSHFSVSAS 87

Query: 81  ASTATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYY 140
           ASTA ETDSLH  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPI+VL ALQNYY
Sbjct: 88  ASTAKETDSLHLNSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPISVLNALQNYY 147

Query: 141 EAYNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGL 200
           +AYNSNVHRGIH+LSAKATDEYE ARKKVASF+NA +A+EI+FTRNATEAINLVAYSWGL
Sbjct: 148 QAYNSNVHRGIHFLSAKATDEYERARKKVASFINAGDAKEIVFTRNATEAINLVAYSWGL 207

Query: 201 ANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKL 260
           ANLKS DEIILTVAEHHSAIVPWQLVAERTGAVLKFV+L EHDVP L DLKEMF+TKTKL
Sbjct: 208 ANLKSGDEIILTVAEHHSAIVPWQLVAERTGAVLKFVNLGEHDVPNLKDLKEMFSTKTKL 267

Query: 261 VVTHHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKM 320
           VVTHHVSNVLASVLPI +IVG AHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKM
Sbjct: 268 VVTHHVSNVLASVLPIGEIVGLAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKM 327

Query: 321 CGPTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLG 380
           CGPTGIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIGLG
Sbjct: 328 CGPTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLG 387

Query: 381 AAVDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIH 440
           AA+DYLSGIGMQKIHSYEEELA YLYE+LRAVPNIRIYGPAPTA+VQRAALCSFN+ +IH
Sbjct: 388 AAIDYLSGIGMQKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIH 447

Query: 441 PTDIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTV 498
           PTDIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSAR SLYFYNTKEDVDYFIQALNDTV
Sbjct: 448 PTDIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARVSLYFYNTKEDVDYFIQALNDTV 507

[Close]

BLAST of Moc02g02080 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 698.0 bits (1800), Expect = 5.6e-201
Identity = 356/474 (75.11%), Postives = 400/474 (84.39%), Query Frame = 0


Query: 24  MEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHFA 83
           ME V +KLPSF    P+  S    S  R        R SS  SV ++A+ ++ T S    
Sbjct: 1   MEGVAMKLPSF----PNAISIGHRSFSR-------VRCSSSLSVCSAAAASSATISTDSE 60

Query: 84  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHY 143
           S+SLGH  R DF ILHQEVNGSKLVYLD+AATSQKP AVL ALQNYYE YNSNVHRGIHY
Sbjct: 61  SVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHY 120

Query: 144 LSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTV 203
           LSAKATDE+ELARKKVA F+NA+++REI+FTRNATEAINLVAYSWGL+NLK  DE+ILTV
Sbjct: 121 LSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTV 180

Query: 204 AEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASV 263
           AEHHS IVPWQ+V+++TGAVLKFV+L+E +VP +  L+E+ + KTKLV  HHVSNVLAS 
Sbjct: 181 AEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASS 240

Query: 264 LPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKS 323
           LPIE+IV WAH  GAKVLVDACQSVPHMVVDVQ L+ADFLVASSHKMCGPTGIGFLYGKS
Sbjct: 241 LPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKS 300

Query: 324 DLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQK 383
           DLL +MPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAI LGAAVDYLSGIGM K
Sbjct: 301 DLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPK 360

Query: 384 IHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHG 443
           IH YE E+ +YLYE L ++P++RIYGP P+ +V R ALCSFNVE +HPTD+ATFLDQQHG
Sbjct: 361 IHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQHG 420

Query: 444 VAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 498
           VAIRSGHHCAQPLHR LGV++SARASLYFYNTK+DVD FI AL DTVSFFNSFK
Sbjct: 421 VAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFFNSFK 463

[Close]

BLAST of Moc02g02080 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-17
Identity = 92/388 (23.71%), Postives = 165/388 (42.53%), Query Frame = 0


Query: 108 VYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDEYELARKKVASFVNAAE 167
           +YLD  AT+     V  A+         N H   H    +A +  E AR +VA  + A+ 
Sbjct: 55  LYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEAS- 114

Query: 168 AREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFV 227
            +EI+F   ATEA N+ A    +   K   + ++T    H  ++      ++ G  + ++
Sbjct: 115 PKEIVFVSGATEANNM-AVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYL 174

Query: 228 SLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQS 287
            +    +  L  L+E     T LV    V+N +  V P+E+I             DA Q+
Sbjct: 175 PVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQA 234

Query: 288 VPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHS 347
           +  + VDV+  +   +  S+HK+ GP G+G LY +      + P + GG           
Sbjct: 235 IGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQ--------- 294

Query: 348 TYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRI 407
                     +GT A  + +G GAA +    + M+++  Y+E+  + L E L      ++
Sbjct: 295 -----ERGLRSGTGATQQIVGFGAACE----LAMKEM-EYDEKWIKGLQERLLNGVREKL 354

Query: 408 YGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHHCA----QPLH--RALG 467
            G     ++    + + N+   +    +  +  +  VA+ SG  C     +P +  RALG
Sbjct: 355 DGVVVNGSMDSRYVGNLNLSFAYVEGESLLMGLKE-VAVSSGSACTSASLEPSYVLRALG 414

Query: 468 VS-----SSARASLYFYNTKEDVDYFIQ 485
           V      +S R  +  + TKE++D  ++
Sbjct: 415 VDEDMAHTSIRFGIGRFTTKEEIDKAVE 420

[Close]

BLAST of Moc02g02080 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 66.6 bits (161), Expect = 6.3e-11
Identity = 66/302 (21.85%), Postives = 127/302 (42.05%), Query Frame = 0


Query: 194 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVT 253
           K   + ++T    H  ++      ++ G  + ++ +    +  L  L+E     T LV  
Sbjct: 11  KDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSI 70

Query: 254 HHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGP 313
             V+N +  V P+E+I             DA Q++  + VDV+  +   +  S+HK+ GP
Sbjct: 71  MAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGP 130

Query: 314 TGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAV 373
            G+G LY +      + P + GG                     +GT A  + +G GAA 
Sbjct: 131 KGVGALYVRRRPRIRLEPLMNGGGQ--------------ERGLRSGTGATQQIVGFGAAC 190

Query: 374 DYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTD 433
           +    + M+++  Y+E+  + L E L      ++ G     ++    + + N+   +   
Sbjct: 191 E----LAMKEM-EYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAYVEG 250

Query: 434 IATFLDQQHGVAIRSGHHCA----QPLH--RALGVS-----SSARASLYFYNTKEDVDYF 485
            +  +  +  VA+ SG  C     +P +  RALGV      +S R  +  + TKE++D  
Sbjct: 251 ESLLMGLKE-VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEIDKA 292

The following BLAST results are available for this feature:

BLAST of Moc02g02080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153071.1	5.9e-269	100.00	cysteine desulfurase 1, chloroplastic [Momordica charantia]	[more]
XP_023513282.1	3.4e-248	91.30	cysteine desulfurase 1, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
KAG6570453.1	2.2e-247	89.68	Protein DGS1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022943969.1	2.8e-247	91.10	cysteine desulfurase 1, chloroplastic-like [Cucurbita moschata]	[more]
XP_022986219.1	7.7e-245	90.63	cysteine desulfurase 1, chloroplastic-like, partial [Cucurbita maxima]	[more]

BLAST of Moc02g02080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WX6	7.9e-200	75.11	Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NFS2 PE...	[more]
Q55793	1.8e-148	63.57	Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX...	[more]
O32164	3.4e-118	52.23	Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) OX=224308 GN=sufS PE...	[more]
Q9K7A0	8.3e-117	53.09	Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM ...	[more]
Q8CTA4	1.1e-113	51.62	Probable cysteine desulfurase OS=Staphylococcus epidermidis (strain ATCC 12228 /...	[more]

BLAST of Moc02g02080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJL0	2.8e-269	100.00	Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111020663 PE=4 SV=1	[more]
A0A6J1FVQ2	1.4e-247	91.10	Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111448537 PE=4 SV=1	[more]
A0A6J1JFG5	3.7e-245	90.63	Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111484037 PE=4 SV=1	[more]
A0A1S3BLT6	2.8e-240	88.66	Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103491447 PE=4 SV=1	[more]
A0A0A0KEG9	8.1e-240	88.09	Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G427980 PE=4 SV=1	[more]

BLAST of Moc02g02080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08490.1	5.6e-201	75.11	chloroplastic NIFS-like cysteine desulfurase	[more]
AT5G65720.1	1.2e-17	23.71	nitrogen fixation S (NIFS)-like 1	[more]
AT5G65720.2	6.3e-11	21.85	nitrogen fixation S (NIFS)-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 92..486 e-value: 7.0E-185 score: 616.4
IPR010970	Cysteine desulfurase, SufS	TIGRFAM	TIGR01979	TIGR01979	coord: 91..493 e-value: 2.6E-176 score: 584.3
IPR010970	Cysteine desulfurase, SufS	CDD	cd06453	SufS_like	coord: 108..486 e-value: 0.0 score: 619.097
IPR000192	Aminotransferase class V domain	PFAM	PF00266	Aminotran_5	coord: 108..482 e-value: 8.5E-143 score: 475.8
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 119..379 e-value: 7.0E-185 score: 616.4
None	No IPR available	PANTHER	PTHR43586	CYSTEINE DESULFURASE	coord: 74..496
None	No IPR available	PANTHER	PTHR43586:SF8	CYSTEINE DESULFURASE 1, CHLOROPLASTIC	coord: 74..496
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 91..489

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g02080.1	Moc02g02080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0051301	cell division
biological_process	GO:0006325	chromatin organization
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0070932	histone H3 deacetylation
biological_process	GO:0030186	melatonin metabolic process
biological_process	GO:0042548	regulation of photosynthesis, light reaction
biological_process	GO:0090042	tubulin deacetylation
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005829	cytosol
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0043014	alpha-tubulin binding
molecular_function	GO:0048487	beta-tubulin binding
molecular_function	GO:0031071	cysteine desulfurase activity
molecular_function	GO:0032041	NAD-dependent histone deacetylase activity (H3-K14 specific)
molecular_function	GO:0051721	protein phosphatase 2A binding
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0042903	tubulin deacetylase activity
molecular_function	GO:0003824	catalytic activity