Moc02g01380 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g01380
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	tRNA-5-taurinomethyluridine 2-sulfurtransferase
Location	chr2: 1047803 .. 1048225 (-)
RNA-Seq Expression	Moc02g01380
Synteny	Moc02g01380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACGCGATGAGGATGGTCATGAAGATATTGCAGTCGTCCACCTAAACGAAGACGATCAAGGCCTTGCAGCTGGGCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGAAAAGGCTCTTGAAATTGCAGGAATGGAAGATAAATCAAAGCTTGGTAAACCAGTAAAGATAAAAGTGAAACCAGAGAGTGATCTGAAAGAACATGAACATAAAGAAGATACAAAAATAGAAATACATGGCGAGTTGAGGAATCAAAGGACCACTGCGGTCGAGCAAATAGGAGAGACATCTTCAGATGAAGCAATCACCCCAGCTCCAATGAAATGGCTTGGAAACCTTGGGAAGAAGTTGTTGCAGATATTTTAA

mRNA sequence

ATGGAACGCGATGAGGATGGTCATGAAGATATTGCAGTCGTCCACCTAAACGAAGACGATCAAGGCCTTGCAGCTGGGCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGAAAAGGCTCTTGAAATTGCAGGAATGGAAGATAAATCAAAGCTTGGTAAACCAGTAAAGATAAAAGTGAAACCAGAGAGTGATCTGAAAGAACATGAACATAAAGAAGATACAAAAATAGAAATACATGGCGAGTTGAGGAATCAAAGGACCACTGCGGTCGAGCAAATAGGAGAGACATCTTCAGATGAAGCAATCACCCCAGCTCCAATGAAATGGCTTGGAAACCTTGGGAAGAAGTTGTTGCAGATATTTTAA

Coding sequence (CDS)

ATGGAACGCGATGAGGATGGTCATGAAGATATTGCAGTCGTCCACCTAAACGAAGACGATCAAGGCCTTGCAGCTGGGCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGAAAAGGCTCTTGAAATTGCAGGAATGGAAGATAAATCAAAGCTTGGTAAACCAGTAAAGATAAAAGTGAAACCAGAGAGTGATCTGAAAGAACATGAACATAAAGAAGATACAAAAATAGAAATACATGGCGAGTTGAGGAATCAAAGGACCACTGCGGTCGAGCAAATAGGAGAGACATCTTCAGATGAAGCAATCACCCCAGCTCCAATGAAATGGCTTGGAAACCTTGGGAAGAAGTTGTTGCAGATATTTTAA

Protein sequence

MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF

Homology

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BLAST of Moc02g01380 vs. NCBI nr
Match: XP_022148831.1 (uncharacterized protein LOC111017390 [Momordica charantia])

HSP 1 Score: 282.7 bits (722), Expect = 1.7e-72
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE
Sbjct: 399 MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA
Sbjct: 459 IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           ITPAPMKWLGNLGKKLLQIF
Sbjct: 519 ITPAPMKWLGNLGKKLLQIF 538

[Close]

BLAST of Moc02g01380 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 222.2 bits (565), Expect = 2.7e-54
Identity = 113/140 (80.71%), Postives = 123/140 (87.86%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           ME +EDG EDIAVVHL+EDDQGLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KA+E
Sbjct: 400 MEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVE 459

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
            A MEDKSKLGKP+KIKVKPES LKE E K+DTK  IHGE  NQ TT ++QIGE SSDEA
Sbjct: 460 NAQMEDKSKLGKPIKIKVKPESVLKESEAKDDTK--IHGESNNQETTVIKQIGEVSSDEA 519

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           IT APMKWLG+L KKLLQ+F
Sbjct: 520 ITTAPMKWLGSLRKKLLQVF 537

[Close]

BLAST of Moc02g01380 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 221.9 bits (564), Expect = 3.6e-54
Identity = 112/140 (80.00%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MER+ED  ED+AVVHL+EDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALE
Sbjct: 399 MERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IA MEDKSKLGKP+KIKVKPE+  KE E K+DTK  IHG L+NQ+TT VEQ GE +SDEA
Sbjct: 459 IAQMEDKSKLGKPIKIKVKPENATKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +T APMKWLG+L KKLLQ+F
Sbjct: 519 LTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Moc02g01380 vs. NCBI nr
Match: XP_023513286.1 (uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.9 bits (564), Expect = 3.6e-54
Identity = 112/140 (80.00%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MER+ED  ED+AVVHL+EDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALE
Sbjct: 399 MERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IA MEDKSKLGKP+KIKVKPE+  KE E K+DTK  IHG L+NQ+TT VEQ GE +SDEA
Sbjct: 459 IAQMEDKSKLGKPIKIKVKPENATKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +T APMKWLG+L KKLLQ+F
Sbjct: 519 LTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Moc02g01380 vs. NCBI nr
Match: KAG6570391.1 (hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 218.8 bits (556), Expect = 3.0e-53
Identity = 111/140 (79.29%), Postives = 123/140 (87.86%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MER+E   ED+AVVHL+EDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALE
Sbjct: 399 MERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IA MEDKSKLGKP+KIKVKPE+  KE E K+DTK  IHG L+NQ+TT VEQ GE +SDEA
Sbjct: 459 IAQMEDKSKLGKPIKIKVKPENASKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +T APMKWLG+L KKLLQ+F
Sbjct: 519 LTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Moc02g01380 vs. ExPASy TrEMBL
Match: A0A6J1D6J8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Momordica charantia OX=3673 GN=LOC111017390 PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 8.2e-73
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE
Sbjct: 399 MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA
Sbjct: 459 IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           ITPAPMKWLGNLGKKLLQIF
Sbjct: 519 ITPAPMKWLGNLGKKLLQIF 538

[Close]

BLAST of Moc02g01380 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.7e-54
Identity = 112/140 (80.00%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MER+ED  ED+AVVHL+EDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALE
Sbjct: 399 MERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IA MEDKSKLGKP+KIKVKPE+  KE E K+DTK  IHG L+NQ+TT VEQ GE +SDEA
Sbjct: 459 IAQMEDKSKLGKPIKIKVKPENATKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +T APMKWLG+L KKLLQ+F
Sbjct: 519 LTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Moc02g01380 vs. ExPASy TrEMBL
Match: A0A6J1JC61 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=LOC111483706 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.9e-53
Identity = 111/140 (79.29%), Postives = 123/140 (87.86%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           MERDED  ED+AVV L+EDDQGLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KALE
Sbjct: 399 MERDEDSDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALE 458

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
           IA MEDKSKLGKP+KIKVKPE+  KE E K+DTK  IHG L+NQ+TT VEQ GE +SDEA
Sbjct: 459 IAQMEDKSKLGKPIKIKVKPENATKECETKDDTK--IHGVLKNQQTTVVEQTGEVASDEA 518

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +T APMKWLG+L KKLLQ+F
Sbjct: 519 LTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of Moc02g01380 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.0e-50
Identity = 105/140 (75.00%), Postives = 118/140 (84.29%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           ME  EDGHED+AVVHL+EDDQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC+KA+E
Sbjct: 401 MEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIE 460

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
            A MEDKSKLGKP+KIKVKPES +KE + K+DT  EIH +LRN+  T VEQI E S DEA
Sbjct: 461 NAQMEDKSKLGKPIKIKVKPESGMKESKAKDDT--EIHDKLRNEEATVVEQIREVSPDEA 520

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +    MKWLG+L KKLLQIF
Sbjct: 521 MATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of Moc02g01380 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 6.3e-49
Identity = 101/140 (72.14%), Postives = 118/140 (84.29%), Query Frame = 0


Query: 1   MERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALE 60
           ME +EDGHED+AVVHL+EDDQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC+KA+E
Sbjct: 402 MEHNEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIE 461

Query: 61  IAGMEDKSKLGKPVKIKVKPESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEA 120
            A MEDKSKLGKP+KIKVKP+S + E + K+DTKI+   E RN+  T VEQ+ + S DEA
Sbjct: 462 NAQMEDKSKLGKPIKIKVKPDSGMNESKAKDDTKIQ--DESRNEEATVVEQMRKVSPDEA 521

Query: 121 ITPAPMKWLGNLGKKLLQIF 141
           +   PMKWLG+L KKLLQIF
Sbjct: 522 MATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of Moc02g01380 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-28
Identity = 58/75 (77.33%), Postives = 65/75 (86.67%), Query Frame = 0


Query: 6   DGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGME 65
           +G  D+AVVHL+EDDQGLAAGQFAAFYEG  CIGSGVILESWDDQ FPVC KAL++A +E
Sbjct: 402 EGDGDVAVVHLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALE 461

Query: 66  DKSKLGKPVKIKVKP 81
           DK+KLGKPVKI   P
Sbjct: 462 DKTKLGKPVKIMTMP 476

The following BLAST results are available for this feature:

BLAST of Moc02g01380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148831.1	1.7e-72	100.00	uncharacterized protein LOC111017390 [Momordica charantia]	[more]
XP_038901654.1	2.7e-54	80.71	tRNA-specific 2-thiouridylase MnmA [Benincasa hispida]	[more]
XP_022944568.1	3.6e-54	80.00	uncharacterized protein LOC111448983 [Cucurbita moschata]	[more]
XP_023513286.1	3.6e-54	80.00	uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo]	[more]
KAG6570391.1	3.0e-53	79.29	hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Moc02g01380 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc02g01380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6J8	8.2e-73	100.00	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Momordica charantia OX=3673 G...	[more]
A0A6J1FY63	1.7e-54	80.00	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JC61	1.9e-53	79.29	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0KX16	2.0e-50	75.00	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7TDN6	6.3e-49	72.14	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Moc02g01380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51310.1	1.2e-28	77.33	transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 2..51 e-value: 1.3E-8 score: 36.3
None	No IPR available	PFAM	PF03054	tRNA_Me_trans	coord: 10..43 e-value: 9.8E-7 score: 27.9
None	No IPR available	PANTHER	PTHR43052:SF3	BNAA08G29550D PROTEIN	coord: 7..93
None	No IPR available	PANTHER	PTHR43052	FAMILY NOT NAMED	coord: 7..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g01380.1	Moc02g01380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061708	tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function	GO:0000049	tRNA binding