Moc02g00970 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc02g00970 vs. NCBI nr
Match: XP_022958149.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 623/905 (68.84%), Postives = 694/905 (76.69%), Query Frame = 0

Query: 1   MLRSPSYLSPSSSSSNKALLVSGKPFVALVGNYEEALFRIEQRVKPNAAPGPAFGLRFAS 60
           MLRSP Y   S SSS +  LV G+P+                 +KP          R A 
Sbjct: 1   MLRSPVYY--SLSSSYQVHLVLGRPY----------------GIKPYTTL-----TRCAP 60

Query: 61  PEYSVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVL 120
           P  SV+KAS  A  + P+SAKAVVTV RP+       +NFG+K+RL DIT LWG KTLVL
Sbjct: 61  P--SVVKASVDAPMDHPLSAKAVVTVHRPLRG---LSLNFGIKQRLHDITDLWGAKTLVL 120

Query: 121 EILSAEIDPTTVLEKGTIKGYGQKVNKEDD-------------------------EHNEE 180
           E+LSAEID  T LEKGTIKGY ++V+++ D                         EHN+E
Sbjct: 121 ELLSAEIDSRTGLEKGTIKGYAERVDRDGDDVITYETKFKVPAEFGSIGAVLVENEHNQE 180

Query: 181 LFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT--------------------- 240
           LFL+DIV+ G+PT PLRFSCNSWL  KSQSDD RIFFTT                     
Sbjct: 181 LFLKDIVVYGIPTAPLRFSCNSWLTPKSQSDDTRIFFTTKSYLPSNTPKGLKRIRAEELK 240

Query: 241 --------KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSD 300
                   KRE HHRIYDYDVYNDLG+ DKG EF+RPVLGGKQ+PYPRRCRTGRAR ++D
Sbjct: 241 KLQGNGYGKRETHHRIYDYDVYNDLGDPDKGVEFQRPVLGGKQHPYPRRCRTGRARCQTD 300

Query: 301 PLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTD 360
            LSEVRS D+YLPRDEAF RI+ V LL  TFYS++N LK  LET + DTK  +    +  
Sbjct: 301 SLSEVRSWDIYLPRDEAFSRIEYVRLLGNTFYSLLNSLK--LETTITDTKFSITEELSNS 360

Query: 361 DVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNP 420
           +   KG WN N +LPKL   VV K VDIL L SSE F KDKFFWFRDEEFARQ LAGLNP
Sbjct: 361 NKGIKGIWNFNAILPKLFSSVVTKAVDILTLPSSETFDKDKFFWFRDEEFARQTLAGLNP 420

Query: 421 YSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE---------------- 480
           YSIRLVKEWPLKS+LEP IYG PESAI+DEIVE+QIKG+MT+ E                
Sbjct: 421 YSIRLVKEWPLKSQLEPDIYGPPESAITDEIVERQIKGVMTLEEAVKQKKLYILDYHDLL 480

Query: 481 ------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATG 540
                 VRKLKGKTLYGSRTLFFLNPD TLRPLAIELS PP+DG+ QWKDV TPCWDATG
Sbjct: 481 VPYVAKVRKLKGKTLYGSRTLFFLNPDGTLRPLAIELSRPPMDGKAQWKDVLTPCWDATG 540

Query: 541 LWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYT 600
           LWLW+I KAHVLAHD+GHHQLVSHWLRTHC VEPYIIA HRQLSAMHPIYRLLHPHFRYT
Sbjct: 541 LWLWKIGKAHVLAHDSGHHQLVSHWLRTHCCVEPYIIATHRQLSAMHPIYRLLHPHFRYT 600

Query: 601 METNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVED 660
           METNAIAR +LINEGGIIETTFSPGKYSMEFSS+AY + WQFNLEALPADLIHRGLAVED
Sbjct: 601 METNAIARSSLINEGGIIETTFSPGKYSMEFSSLAYRQHWQFNLEALPADLIHRGLAVED 660

Query: 661 PNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRT 720
           PNAPHGLKL+IEDYPYANDGLILWDAIKEWA EYVNHYYPDPSVVKSD+ELQAWWTEIR 
Sbjct: 661 PNAPHGLKLSIEDYPYANDGLILWDAIKEWAMEYVNHYYPDPSVVKSDEELQAWWTEIRE 720

Query: 721 VGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVN 780
           VGHGDKK+EPWW VL+TP+DLID+VTTIMW+T+GHHAV NFGQYSFAKYFPNRPSIAR N
Sbjct: 721 VGHGDKKEEPWWPVLDTPKDLIDIVTTIMWITTGHHAVANFGQYSFAKYFPNRPSIARAN 780

Query: 781 MPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWA 830
           +PTE+P K WKYFLEKPENVFL+TFPTQ QATIVSSV ++L+SH  DEKYLGKD+E AWA
Sbjct: 781 VPTEQPKKHWKYFLEKPENVFLETFPTQIQATIVSSVASVLASHPPDEKYLGKDMEAAWA 840

BLAST of Moc02g00970 vs. NCBI nr
Match: XP_022148734.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 599/909 (65.90%), Postives = 683/909 (75.14%), Query Frame = 0

Query: 1   MLRSPSYLSPSSSSSNKALLVSGKPFVALVGNYEEALFRI-EQRVKPNAAPGPAFGLRFA 60
           ML+SP Y      +S +AL +   PF+      E  L  I  Q VKPNAAP     L F 
Sbjct: 1   MLKSPVY------NSKQALCIYRGPFLLSNNVAEPNLLPIGRQSVKPNAAP----RLCFD 60

Query: 61  SPEYSVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL- 120
           SP  +V  ++A  TGN PVSA  VVT+KRP      FP        +  ITH W TKT  
Sbjct: 61  SPNSAVKSSAATPTGN-PVSAAVVVTLKRPP-----FP-------SIDGITHFWATKTKP 120

Query: 121 -VLEILSAEIDPTTVLEKGTIKGYGQKVNKED-------------------------DEH 180
            VLE+LSA++DPTT LEKGTIK Y +KV +ED                         ++H
Sbjct: 121 PVLELLSADLDPTTGLEKGTIKVYAKKVAREDAEVIMYDIKFVIPADFGAIGAVLVENQH 180

Query: 181 NEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT------------------ 240
           N+ELFL+DIVIQGLPTGP+RFSCNSWL+ KS S DRRIFFTT                  
Sbjct: 181 NKELFLKDIVIQGLPTGPVRFSCNSWLSPKSHSGDRRIFFTTKSYLPSNTPDGLKRFRAK 240

Query: 241 -----------KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARY 300
                      KREAHHRIYDYDVYNDLG+ D GDE+KRPVLGGKQ+PYPRRCRTGR+R 
Sbjct: 241 ELRNLQDNGSGKREAHHRIYDYDVYNDLGDPDNGDEYKRPVLGGKQHPYPRRCRTGRSRS 300

Query: 301 RSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLS 360
            +DPLSEVRSL+VY+PRDE FL IKQV +LAK    M++ + P LE    DT L   HLS
Sbjct: 301 PTDPLSEVRSLNVYVPRDETFLEIKQVNMLAKAVSWMLHCVVPTLEA---DTNLCSSHLS 360

Query: 361 TTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAG 420
            T+++ +K TWNLNTLL +L+DFV  K VD+LP + S+ F  DKFFWFRDEEFARQ LAG
Sbjct: 361 NTNNL-SKHTWNLNTLLRRLVDFVAHKVVDVLP-SPSQTFNGDKFFWFRDEEFARQTLAG 420

Query: 421 LNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE------------- 480
           LNPYSIRLV EWP+KSKL+P IYG PESAI+DEIVE+QIKG MT++E             
Sbjct: 421 LNPYSIRLVTEWPIKSKLDPCIYGPPESAITDEIVEEQIKGFMTLDEAVKQKKLFALDYH 480

Query: 481 ---------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWD 540
                    VRKLKGKTLYGSRTLFFLNPDNTL+PLAIEL+ PP + +PQWKDVF PC D
Sbjct: 481 DLFLPYVTKVRKLKGKTLYGSRTLFFLNPDNTLKPLAIELTRPPTNAKPQWKDVFKPCCD 540

Query: 541 ATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHF 600
           ATGLWLWRIAKAHV+AHD G+HQLVSHWLRTHCSVEPYIIA HRQLSAMHPIYRLLHPHF
Sbjct: 541 ATGLWLWRIAKAHVVAHDFGYHQLVSHWLRTHCSVEPYIIATHRQLSAMHPIYRLLHPHF 600

Query: 601 RYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLA 660
           R T+E NA AR+TLIN  GIIET FSPGKYSME +SV Y KQWQFNLEALPADLI+RGLA
Sbjct: 601 RGTIEINAAARKTLINVEGIIETAFSPGKYSMEITSVVYQKQWQFNLEALPADLINRGLA 660

Query: 661 VEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTE 720
           VEDPNAPHGLKLTIEDYP+AND LILW+AIK+WATEYVNHYYP+P+++K D+ELQAWW E
Sbjct: 661 VEDPNAPHGLKLTIEDYPFANDALILWEAIKQWATEYVNHYYPEPNLIKLDEELQAWWME 720

Query: 721 IRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIA 780
           IRTVGHGDKKDEPWW +LNTP+DLIDVVTTIMWVTSGHHA VNFGQY F +YFP RPS A
Sbjct: 721 IRTVGHGDKKDEPWWPILNTPKDLIDVVTTIMWVTSGHHAAVNFGQYFFTRYFPIRPSFA 780

Query: 781 RVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIE 830
           RV++PTE+P  +LWKYFLE PE VF DTFPT  +A+++  ++NILS+H  DEKYLG ++E
Sbjct: 781 RVDVPTEDPNSRLWKYFLENPEKVFSDTFPTHVEASVLLCILNILSTHPPDEKYLGGEME 840

BLAST of Moc02g00970 vs. NCBI nr
Match: XP_022148713.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 567/836 (67.82%), Postives = 649/836 (77.63%), Query Frame = 0

Query: 77  PVSAKAVVTVKRPVGNGELFPINFGLKRRLPDIT-HLWGTKTLVLEILSAEIDPTTVLEK 136
           P SA  VVTVKR V  G L   N GLKR L D+T +LWGTKTLV E+LS E+DPTT LEK
Sbjct: 4   PFSAHVVVTVKRHV--GRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEK 63

Query: 137 GTIKGYGQKVNKEDD---------------------------EHNEELFLEDIVIQGLPT 196
           GTIKGY QKV +E++                           ++N ELFL+DIVI G+P 
Sbjct: 64  GTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPY 123

Query: 197 GPLRFSCNSWLNSKSQSDDRRIFFTT-----------------------------KREAH 256
           GPLRFSCNSWL+ KS S DRRIFFTT                             KRE+H
Sbjct: 124 GPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESH 183

Query: 257 HRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLP 316
           HRIYDYDVYNDLG+ D+GDEFKRP+LGGKQ+PYPRRCRTGRAR ++DPLSEV S + Y+P
Sbjct: 184 HRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYVP 243

Query: 317 RDEAFLRIKQVTLLA-KTFYSMINGLKPALETIVPDTKLHVPHLSTTDDVP-NKGTWNLN 376
           RDEAF+  KQVT  A KT YSM++G  PALE I  D +    +      +P +K TW  +
Sbjct: 244 RDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVML--LPSSKYTWEPS 303

Query: 377 TLLPKLIDFVVDKGVDILPLASSEN-FGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWP 436
            LLP+LIDF VDK V IL L  S+     DKFFWF+DEEFARQ LAGLNP SIRLV EWP
Sbjct: 304 ALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWP 363

Query: 437 LKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE----------------------VRKL 496
           L+SKL+PAIYG  +SAI++EIVEQQIKG MT+NE                      VRK+
Sbjct: 364 LESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNEAVEQKKLYILDYHDLFLPYVAKVRKI 423

Query: 497 KGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATGLWLWRIAKAH 556
           +GKTLYGSRTLFFLNPDNTL+PLAIEL+ PPIDG+ QWKDVFTPC ++TGLWLWRIAKAH
Sbjct: 424 EGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAH 483

Query: 557 VLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQT 616
           VLAHD+G+HQLVSHWLRTHCSVEPYIIA HRQLSAMHPIYRLLHPHFRYTME NAIAR+ 
Sbjct: 484 VLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREA 543

Query: 617 LINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLT 676
           L N GGIIET FS GKYSME SS+ Y KQWQFNLEALPADLIHRG+AVEDPNA HG+KL+
Sbjct: 544 LTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLS 603

Query: 677 IEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEP 736
           IEDYP+ANDGLILWDAIKEWATEYVNHYYPDP VVKSD ELQAWWTEIR+VGHGDKK+EP
Sbjct: 604 IEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEP 663

Query: 737 WWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKL 796
           WW +LNTPEDLIDVV+++MWVTSGHHA VNFGQY FA YFP RPS+AR+N+PTEEP  KL
Sbjct: 664 WWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKL 723

Query: 797 WKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWADEPHIKRAF 830
           WK FLE PE VFLDTFP   QAT++ S+++ILS+HS +E+YLG+D+EPAW D+P +K+AF
Sbjct: 724 WKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAF 783

BLAST of Moc02g00970 vs. NCBI nr
Match: XP_022943468.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 555/851 (65.22%), Postives = 646/851 (75.91%), Query Frame = 0

Query: 64  SVIKASAVATG-NKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEI 123
           +VIKAS+VA+   KP+S KA+VTV+R +G+G        L+R L DI  L+G K+LV+E+
Sbjct: 59  AVIKASSVASSTEKPLSVKALVTVQRTLGSG------LYLERGLDDIADLFG-KSLVVEL 118

Query: 124 LSAEIDPTTVLEKGTIKGYGQKVNKEDD------------------------EHNEELFL 183
           +SAE+DP T LEK TI+ Y  K + E D                        EH++E+++
Sbjct: 119 VSAEVDPATGLEKATIRHYAHKEDTEGDEITYEADFKIPPDFGIIGAIFVENEHHKEMYV 178

Query: 184 EDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT------------------------ 243
           +DIV++G P GPL F C+SW+N K   D +R+FFTT                        
Sbjct: 179 KDIVLEGHPIGPLHFVCSSWVNEKDLPDSKRVFFTTKSYLPSNTPEGLKKLREEELKAVR 238

Query: 244 -----KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLS 303
                KRE+H RIYDYDVYNDLG+ +K  E KRPVLGGKQ+PYPRRCRTGR R   DP+S
Sbjct: 239 GNGVGKRESHERIYDYDVYNDLGDPNKNPEHKRPVLGGKQFPYPRRCRTGRPRASRDPVS 298

Query: 304 EVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDV- 363
           E RSL+ Y+PRDEAF  +KQ T  A +  S++NGL PALE++  D  +  PH    DD+ 
Sbjct: 299 ESRSLNNYVPRDEAFSALKQATFNASSLNSVLNGLIPALESVSTDNDIRFPHFPAIDDLF 358

Query: 364 -------PNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPL 423
                  P+KG W L TLLP++ID VVD   DIL     E F +DKFFWFRDEEF RQ L
Sbjct: 359 DNGVPLPPSKGGWKLATLLPRIIDTVVDSAEDILRFHPPETFDRDKFFWFRDEEFGRQTL 418

Query: 424 AGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE----------- 483
           AGLNPYS+RLV EWPLKSKL+PA+YG PES I+ +I+EQQI+G MT++E           
Sbjct: 419 AGLNPYSLRLVTEWPLKSKLDPAVYGPPESEITGKIIEQQIRGFMTLDEALTKKRLFILD 478

Query: 484 -----------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPC 543
                      VRKLKG TLYGSRTLFFL+ D+TL+PLAIEL+ PPIDG+PQWK VFTP 
Sbjct: 479 YHDLLLPYVAKVRKLKGTTLYGSRTLFFLHSDSTLKPLAIELTRPPIDGKPQWKKVFTPF 538

Query: 544 WDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHP 603
           WDAT +WLWRIAKAHVLAHD+G+HQLVSHWLRTHC VEPYIIA++RQLSAMHPIYRLLHP
Sbjct: 539 WDATSVWLWRIAKAHVLAHDSGYHQLVSHWLRTHCCVEPYIIASNRQLSAMHPIYRLLHP 598

Query: 604 HFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRG 663
           HFRYTME NA+ARQ LIN  GIIET+FSPGKYSMEFSSVAY +QWQFNLEALPADLIHRG
Sbjct: 599 HFRYTMEINALARQALINANGIIETSFSPGKYSMEFSSVAYKEQWQFNLEALPADLIHRG 658

Query: 664 LAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWW 723
           LAVEDPNAPHGLKLTIEDYP+ANDGLILWDAIK+WATEYVN+YYPDPS+VKSD+ELQAWW
Sbjct: 659 LAVEDPNAPHGLKLTIEDYPFANDGLILWDAIKDWATEYVNYYYPDPSLVKSDEELQAWW 718

Query: 724 TEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPS 783
           TEIRT GH DKKDEPWW VLNTPEDLI VVTTIMWVTSGHHA VNFGQYSFA YFPNRPS
Sbjct: 719 TEIRTEGHADKKDEPWWPVLNTPEDLISVVTTIMWVTSGHHAAVNFGQYSFAGYFPNRPS 778

Query: 784 IARVNMPTEEPMK-LWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKD 830
           IAR+N+P EE  K  W+YFL KPE+V L+TFPTQ QAT V++V+NILSSHS DE+Y+GKD
Sbjct: 779 IARINVPVEEKNKEKWEYFLNKPEDVLLETFPTQLQATKVTAVLNILSSHSPDEEYMGKD 838

BLAST of Moc02g00970 vs. NCBI nr
Match: KAG6570374.1 (Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 554/851 (65.10%), Postives = 645/851 (75.79%), Query Frame = 0

Query: 64  SVIKASAVATG-NKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEI 123
           +VIKAS+VA+   KP+S K +VTV+R +G+G        L+R L DI  L+G K+LV+E+
Sbjct: 59  AVIKASSVASSTEKPLSVKVLVTVQRTLGSG------LYLERGLDDIADLFG-KSLVVEL 118

Query: 124 LSAEIDPTTVLEKGTIKGYGQKVNKEDD------------------------EHNEELFL 183
           +SAE+DP T LEK TI+ Y  K + E D                        EH++E+++
Sbjct: 119 VSAEVDPATGLEKATIRHYAHKEDTEGDEITYEADFKIPPDFGIIGAIFVENEHHKEIYV 178

Query: 184 EDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT------------------------ 243
           +DIV++G P GPL F C+SW+N K   D +R+FFTT                        
Sbjct: 179 KDIVLEGHPIGPLHFVCSSWVNEKDLPDSKRVFFTTKSYLPSNTPEGLKKLRQEELKAVR 238

Query: 244 -----KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLS 303
                KRE+H RIYDYDVYNDLG+ +K  E KRPVLGGKQ+PYPRRCRTGR R   DP+S
Sbjct: 239 GNGVGKRESHERIYDYDVYNDLGDPNKNPEHKRPVLGGKQFPYPRRCRTGRPRASRDPVS 298

Query: 304 EVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDV- 363
           E RSL+ Y+PRDEAF  +KQ T  A +  S++NGL PALE++  D  +  PH    DD+ 
Sbjct: 299 ESRSLNNYVPRDEAFSALKQATFNASSLNSVLNGLIPALESVSTDNDIRFPHFPAIDDLF 358

Query: 364 -------PNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPL 423
                  P+KG W L TLLP++ID VVD   DIL     E F +DKFFWFRDEEF RQ L
Sbjct: 359 DNGVPLPPSKGGWKLATLLPRIIDTVVDSAEDILRFHPPETFDRDKFFWFRDEEFGRQTL 418

Query: 424 AGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE----------- 483
           AGLNPYS+RLV EWPLKSKL+PA+YG PES I+ +I+EQQI+G MT++E           
Sbjct: 419 AGLNPYSLRLVTEWPLKSKLDPAVYGPPESEITGKIIEQQIRGFMTLDEALTKKRLFILD 478

Query: 484 -----------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPC 543
                      VRKLKG TLYGSRTLFFL+ D+TL+PLAIEL+ PPIDG+PQWK VFTP 
Sbjct: 479 YHDLLLPYVAKVRKLKGTTLYGSRTLFFLHSDSTLKPLAIELTRPPIDGKPQWKKVFTPF 538

Query: 544 WDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHP 603
           WDAT +WLWRIAKAHVLAHD+G+HQLVSHWLRTHC VEPYIIA++RQLSAMHPIYRLLHP
Sbjct: 539 WDATSVWLWRIAKAHVLAHDSGYHQLVSHWLRTHCCVEPYIIASNRQLSAMHPIYRLLHP 598

Query: 604 HFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRG 663
           HFRYTME NA+ARQ LIN  GIIET+FSPGKYSMEFSSVAY +QWQFNLEALPADLIHRG
Sbjct: 599 HFRYTMEINALARQALINANGIIETSFSPGKYSMEFSSVAYKEQWQFNLEALPADLIHRG 658

Query: 664 LAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWW 723
           LAVEDPNAPHGLKLTIEDYP+ANDGLILWDAIK+WATEYVN+YYPDPS+VKSD+ELQAWW
Sbjct: 659 LAVEDPNAPHGLKLTIEDYPFANDGLILWDAIKDWATEYVNYYYPDPSLVKSDEELQAWW 718

Query: 724 TEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPS 783
           TEIRT GH DKKDEPWW VLNTPEDLI VVTTIMWVTSGHHA VNFGQYSFA YFPNRPS
Sbjct: 719 TEIRTEGHADKKDEPWWPVLNTPEDLISVVTTIMWVTSGHHAAVNFGQYSFAGYFPNRPS 778

Query: 784 IARVNMPTEEPMK-LWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKD 830
           IAR+N+P EE  K  W+YFL KPE+V L+TFPTQ QAT V++V+NILSSHS DE+Y+GKD
Sbjct: 779 IARINVPVEEKNKEKWEYFLNKPEDVLLETFPTQLQATKVTAVLNILSSHSPDEEYMGKD 838

BLAST of Moc02g00970 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 1.9e-267
Identity = 470/905 (51.93%), Postives = 605/905 (66.85%), Query Frame = 0

Query: 12  SSSSNKALLVSGKPFVALVGNYEEALFRIEQRVKPNAAPGPAFGLRFASPEYSVIKASAV 71
           SS S KAL+ S       + ++   +     R+K             AS   +V+ ++  
Sbjct: 9   SSQSTKALIPSWNTNPLFLASFPINILNKNFRLKKKNNFRVHHNYNGASTTKAVLSSTEK 68

Query: 72  ATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEILSAEIDPTT 131
           ATG      KAVVTV++ V        N  L R L DI  L G K+L+L I++AE+D  T
Sbjct: 69  ATG-----VKAVVTVQKQV--------NLNLSRGLDDIGDLLG-KSLLLWIVAAELDHKT 128

Query: 132 VLEKGTIKGY---GQKVNKE---------------------DDEHNEELFLEDIVIQGLP 191
            +EK  I+ Y   G+ V+ +                     ++EH++E+++++IVI G  
Sbjct: 129 GIEKPGIRAYAHRGRDVDGDTHYEADFVIPQDFGEVGAILIENEHHKEMYVKNIVIDGFV 188

Query: 192 TGPLRFSCNSWLNSKSQSDDRRIFFTTK-----------------------------REA 251
            G +  +CNSW++SK  + D+RIFFT K                             R+ 
Sbjct: 189 HGKVEITCNSWVHSKFDNPDKRIFFTNKSYLPSQTPSGVSRLREEELVTLRGDGIGERKV 248

Query: 252 HHRIYDYDVYNDLGNSD-KGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVY 311
             RIYDYDVYNDLG +D   D+ KRPVLGGK+ PYPRRC+TGR R + DPLSE RS  VY
Sbjct: 249 FERIYDYDVYNDLGEADSNNDDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETRSTFVY 308

Query: 312 LPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDVPNKGT---- 371
           +PRDEAF  +K V     T YS+++ + PALE++V D  L  PH    D + N G     
Sbjct: 309 VPRDEAFSEVKSVAFSGNTVYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFNVGVDLPG 368

Query: 372 -----WNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYS 431
                  L  ++P+LI  + D   D+L   S +   +DKF WFRD EFARQ LAGLNPYS
Sbjct: 369 LGDKKSGLFNVVPRLIKAISDTRKDVLLFESPQLVQRDKFSWFRDVEFARQTLAGLNPYS 428

Query: 432 IRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMT--------------------- 491
           IRLV EWPL+SKL+P +YG PES I+ E++E++I   MT                     
Sbjct: 429 IRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQAVQQKKLFILDYHDLLLP 488

Query: 492 -VNEVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTP-CWDATGL 551
            VN+V +LKG  LYGSRT+FFL P  TL+PLAIEL+ PP+D +PQWK+V++P  W+ATG 
Sbjct: 489 YVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDKPQWKEVYSPNDWNATGA 548

Query: 552 WLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTM 611
           WLW++AKAHVL+HD+G+HQLVSHWLRTHC  EPYIIA++RQLSAMHPIYRLLHPHFRYTM
Sbjct: 549 WLWKLAKAHVLSHDSGYHQLVSHWLRTHCCTEPYIIASNRQLSAMHPIYRLLHPHFRYTM 608

Query: 612 ETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDP 671
           E NA+AR+ LIN  G+IE++F PGKY++E SS+AY  +W+F+ EALP +LI RGLAVEDP
Sbjct: 609 EINALAREALINANGVIESSFFPGKYAIELSSIAYGAEWRFDQEALPQNLISRGLAVEDP 668

Query: 672 NAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTV 731
           N PHGLKL IEDYP+ANDGL+LWD +K+W T YVNHYYP  ++++SD ELQAWW+EI+ V
Sbjct: 669 NEPHGLKLAIEDYPFANDGLVLWDILKQWVTNYVNHYYPQTNLIESDKELQAWWSEIKNV 728

Query: 732 GHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNM 791
           GHGDK+DEPWW  L TP DLI ++TTI+WVTSGHHA VNFGQYS+A YFPNRP++AR  M
Sbjct: 729 GHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKM 788

Query: 792 PTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWA 830
           PTE+P  + W++F+ KPE   L  FP+Q QAT V +++++LS+HS DE+Y+G+ IEP WA
Sbjct: 789 PTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWA 848

BLAST of Moc02g00970 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 3.2e-246
Identity = 438/855 (51.23%), Postives = 569/855 (66.55%), Query Frame = 0

Query: 64  SVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEIL 123
           S IKA    +  K +  K VVTV +P  +G L  +  GL   + D    +  ++ +LE++
Sbjct: 54  SNIKAIMPDSIGKSIRKKCVVTV-QPTISGALTAVTVGLLGTVADSVSDFLGRSFLLELV 113

Query: 124 SAEIDPTTVLEKGTIKGYG--QKVNKE------------------------DDEHNEELF 183
           S+++D ++  EK T+K Y    +++KE                         +E + + +
Sbjct: 114 SSDLD-SSGKEKDTVKAYATYDELDKESKLYKYQCEFEVPDDFGEIGAVLVQNERHRDAY 173

Query: 184 LEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTK---------------------- 243
           +++IV+  + T    F+C+SW++SK  + D+RIFF  K                      
Sbjct: 174 VKNIVLDEIVT----FTCDSWIHSKFDNPDKRIFFLNKSYLPSETPEGLKSLRQKDLESL 233

Query: 244 -------REAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPL 303
                  R++  RIYDYD YND+G+ D   +  RPVLGG ++P+PRRCRTGR    ++P 
Sbjct: 234 RGNGEGERQSFDRIYDYDTYNDIGDPDTDSDMARPVLGGNEHPFPRRCRTGRKMTSTEPW 293

Query: 304 SEVR-SLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLS---- 363
           SE R +L  Y+PRDE F  IKQ+T  A T YS+++G+ PAL +++ D     P       
Sbjct: 294 SESRTTLPFYVPRDEDFAEIKQITRGATTLYSVLHGVIPALSSVLKDEDKGFPLFRDIEL 353

Query: 364 ------TTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFA 423
                   D  P+ GT    + LP+L+  + +    +L   +     KD F WFRDEEF 
Sbjct: 354 LYEKGVDIDPPPDSGTL---SALPRLVKAITNSTKKVLQFETPRIKHKDSFSWFRDEEFC 413

Query: 424 RQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMT---------- 483
           RQ LAGLNPYSI+LV EWPL SKL+P +YG  ESAI+ E VE++IKG MT          
Sbjct: 414 RQTLAGLNPYSIQLVTEWPLMSKLDPEVYGPAESAITKETVEEEIKGFMTXEEALEQKRL 473

Query: 484 ------------VNEVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDV 543
                       VN+VR+++G TLYGSRTL FL    TLRPLAIEL+ PP +G+PQWK V
Sbjct: 474 FLLDYHDLLLPYVNKVREIEGTTLYGSRTLMFLTCTGTLRPLAIELTRPPNNGKPQWKHV 533

Query: 544 FTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYR 603
           +TPCWDAT  WLW++AKAHVLAHD+G+HQLVSHWLRTHC  EPYIIA +RQLS MHPI R
Sbjct: 534 YTPCWDATDXWLWKLAKAHVLAHDSGYHQLVSHWLRTHCVTEPYIIATNRQLSKMHPIQR 593

Query: 604 LLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADL 663
           LL PH RYTM+ N +AR +LIN  GIIE++FSP KYSM+ SS AYA++W+F+ EALPADL
Sbjct: 594 LLCPHLRYTMQINGLARLSLINANGIIESSFSPRKYSMQLSSDAYAQKWRFDHEALPADL 653

Query: 664 IHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDEL 723
           I RG+AVED +APHG+KLTIEDYP+ANDGL+LWDAIK+WAT Y+NHYYP   +V+SD+EL
Sbjct: 654 ISRGMAVEDESAPHGIKLTIEDYPFANDGLLLWDAIKQWATAYINHYYPQAKLVESDEEL 713

Query: 724 QAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFP 783
           QAWWTEIRTVGH DKKDEPWW  L T +DLI VV+TIMWV+SGHH+ VNFGQY F  YFP
Sbjct: 714 QAWWTEIRTVGHADKKDEPWWPQLKTQQDLIGVVSTIMWVSSGHHSAVNFGQYDFGGYFP 773

Query: 784 NRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKY 830
           NRP+IAR  MP E+P  + W+ F+EKPE+V L+ FPTQ QAT V +++++LSSHS DE+Y
Sbjct: 774 NRPTIARTKMPNEDPTFEEWEAFMEKPEDVLLNCFPTQIQATKVMAILDVLSSHSPDEEY 833

BLAST of Moc02g00970 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 4.5e-232
Identity = 418/839 (49.82%), Postives = 534/839 (63.65%), Query Frame = 0

Query: 85  TVKRPVGN---------GELFPINFGLKRRLPDITHLWGTKTLVLEILSAEIDPTTVLEK 144
           TVK P+ N          E F       R L DI  + G ++L++E++SA+ D     ++
Sbjct: 65  TVKEPIQNIKVKGYITAQEEFLEGITWSRGLDDIADIRG-RSLLVELISAKTD-----QR 124

Query: 145 GTIKGYGQKVNKE------------------------DDEHNEELFLEDIVIQGLPTGPL 204
            T++ Y Q+V  E                         ++++ +LFL+ + ++ LP G +
Sbjct: 125 ITVEDYAQRVWAEAPDEKYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSI 184

Query: 205 RFSCNSWLNSKSQSDDRRIFFTTK--------------------------RE------AH 264
            F+C SW+  KS    +RIFF+ K                          RE        
Sbjct: 185 TFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKF 244

Query: 265 HRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVR-SLDVYL 324
            RIYDYDVYND+G+ D   E  RPV+GG  +PYPRRC+TGR    +DP SE R   + Y+
Sbjct: 245 ERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYV 304

Query: 325 PRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDVPNKGTW---- 384
           PRDE F   K  +   K   + +  + P +E+++   +   PH     ++  +G      
Sbjct: 305 PRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKD 364

Query: 385 -NLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVK 444
             L  LLP++I  + +   DIL   +     +D+F W RD+EFARQ LAGLNPYSI+LV+
Sbjct: 365 AGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVE 424

Query: 445 EWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMT----------------------VNEV 504
           EWPL SKL+PA+YG P S I+ EIVE+++KG MT                      VN+V
Sbjct: 425 EWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKV 484

Query: 505 RKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATGLWLWRIA 564
           R+L   TLY SRTLFFL+ D+TLRP+AIEL+ PP   +PQWK VFTP +DAT  WLW +A
Sbjct: 485 RELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWNLA 544

Query: 565 KAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIA 624
           K H ++HD G+HQL+SHWLRTH   EPYIIAA+RQLSAMHPIYRLLHPHFRYTME NA A
Sbjct: 545 KTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARA 604

Query: 625 RQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGL 684
           RQ+L+N GGIIET F PGKY++E SS  Y K W+F+ E LPADLI RGLA ED  A HG+
Sbjct: 605 RQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGV 664

Query: 685 KLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKK 744
           +LTI DYP+ANDGLILWDAIKEW T+YV HYYPD  ++ SD+ELQ WW+E+R +GHGDKK
Sbjct: 665 RLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKK 724

Query: 745 DEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPM 804
           DEPWW VL T +DLI VVTTI WVTSGHHA VNFGQY +  YFPNRP+  R+ MPTE+P 
Sbjct: 725 DEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPT 784

Query: 805 -KLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWADEPHIK 830
            +  K F E PE V L T+P+Q QAT+V   +++LS+HS DE+Y+G+  E +WA+EP I 
Sbjct: 785 DEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVIN 844

BLAST of Moc02g00970 vs. ExPASy Swiss-Prot
Match: Q8GSM2 (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 7.7e-224
Identity = 411/857 (47.96%), Postives = 542/857 (63.24%), Query Frame = 0

Query: 59  ASPEYSVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL 118
           AS E +V  +++V T  + ++  A+VT +         P +  + R L DI  L+G KTL
Sbjct: 51  ASTEEAVGVSTSVTTKERALTVTAIVTAQ--------VPTSVYVARGLDDIQDLFG-KTL 110

Query: 119 VLEILSAEIDPTTVLEKGTIKGYGQKVNKE---------------------DDEHNEELF 178
           +LE++S+E+DP T  E+  +KG+     KE                     ++EH+ E+F
Sbjct: 111 LLELVSSELDPKTGRERERVKGFAHMTLKEGTYEAKMSVPASFGPVGAVLVENEHHREMF 170

Query: 179 LEDI--VIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTK-------------------- 238
           ++DI  +  G  +  + F   SW++SK    + R FFT K                    
Sbjct: 171 IKDIKLITGGDESTAITFDVASWVHSKFDDPEPRAFFTVKSYLPSQTPPGIEALRKKELE 230

Query: 239 ---------REAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSD 298
                    R+ H R+YDYD YNDLG+ DK  + KRPVLG K++PYPRRCRTGR +   D
Sbjct: 231 TLRGDGHSERKFHERVYDYDTYNDLGDPDKNIDHKRPVLGTKEHPYPRRCRTGRPKTLYD 290

Query: 299 PLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTK--LHVPHLST 358
           P +E RS  VY+PRDE F  +K  T  A T  S ++ + PA+  ++ ++    H P +  
Sbjct: 291 PETETRSSPVYVPRDEQFSDVKGRTFSATTLRSGLHAILPAVAPLLNNSHGFSHFPAIDA 350

Query: 359 --TDDVP------NKGTWN-LNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEE 418
             +D +P         ++N +N ++P+++  + D    +L     E   +D+F WFRDEE
Sbjct: 351 LYSDGIPLPVDGHGGNSFNVINDVIPRVVQMIEDTTEHVLRFEVPEMLERDRFSWFRDEE 410

Query: 419 FARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE----- 478
           FARQ LAGLNP  IR + E+P+ SKL+PA+YG  ESA+S EI+E+ + G MTV E     
Sbjct: 411 FARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKMMNGRMTVEEAMEKK 470

Query: 479 -----------------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWK 538
                            VR+L   TLYGSRT+FFL+ + TL PLAIEL+ P    +PQWK
Sbjct: 471 RLFLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFLSDEGTLMPLAIELTRPQSPTKPQWK 530

Query: 539 DVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPI 598
             FT   DAT  WLW++AKAHVL HDTG+HQLVSHWLRTH  VEPYIIA +RQLS MHP+
Sbjct: 531 RAFTHGSDATESWLWKLAKAHVLTHDTGYHQLVSHWLRTHACVEPYIIATNRQLSRMHPV 590

Query: 599 YRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPA 658
           YRLLHPHFRYTME NA+AR+ LIN  GIIE  F  GKYS+E SSVAY   WQFN EALP 
Sbjct: 591 YRLLHPHFRYTMEINALAREALINADGIIEEAFLAGKYSIELSSVAYGAAWQFNTEALPE 650

Query: 659 DLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDD 718
           DLI+RGLAV   +    L+L I+DYPYA+DGL++W +IK+WA++YV+ YY     V  D+
Sbjct: 651 DLINRGLAVRRDDGE--LELAIKDYPYADDGLLIWGSIKQWASDYVDFYYKSDGDVAGDE 710

Query: 719 ELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKY 778
           EL+AWW E+RT GH DKKDEPWW V +T E+L+ ++T IMWVTSGHHA VNFGQY +A Y
Sbjct: 711 ELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAVNFGQYHYAGY 770

Query: 779 FPNRPSIARVNMPTEEPM-KLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDE 830
           FPNRP++ R N+P EE      K F+ +PE V L + P+Q QA  V + ++ILSSHS DE
Sbjct: 771 FPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPDE 830

BLAST of Moc02g00970 vs. ExPASy Swiss-Prot
Match: Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 5.0e-223
Identity = 408/850 (48.00%), Postives = 537/850 (63.18%), Query Frame = 0

Query: 78  VSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEILSAEIDPTTVLEKGT 137
           V  KAV T+K  V   E    +    + + +I  L G ++L LE++S+E++  T  +K T
Sbjct: 96  VKVKAVATIKVTV---EGLLNSLRPSKAIDNIRDLIG-RSLFLELVSSELEAKTGKKKAT 155

Query: 138 IKGYGQKVNKED-------------------------DEHNEELFLEDI-VIQGLPTG-- 197
           +  Y  KV+ +D                         +E  +E+FLED+ +  G   G  
Sbjct: 156 VHSYAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVVTNELGQEMFLEDLNLTAGDGAGNS 215

Query: 198 -PLRFSCNSWLNSKSQSDD----RRIFFTT----------------------------KR 257
             L   CNSW+  KS  D+    +RIFF                              +R
Sbjct: 216 TVLPIRCNSWVQPKSSIDEGTPGKRIFFAKAYLPGQTPAGLRSYREEDLKQKRGNGAGQR 275

Query: 258 EAHHRIYDYDVYNDLGNSDKGDEFKRPVLGG-KQYPYPRRCRTGRARYRSDPLSEVRSLD 317
           EA  R+YDYDVYNDLGN D   +  RPVLGG KQ+PYPRRCRTGR   + DP SE R  +
Sbjct: 276 EADDRVYDYDVYNDLGNPDSNGDLARPVLGGSKQFPYPRRCRTGRPPSKKDPKSETRKGN 335

Query: 318 VYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALET-IVPDTKLHVPHLS-------TTDD 377
           VY+PRDE F  +K    L KT  S+++   PA ++ ++ +  L++P  S         D 
Sbjct: 336 VYVPRDEEFSEVKNAQFLLKTLQSVLHAAVPAAQSALIDNLSLNLPFPSFFVIDKLFEDG 395

Query: 378 VPNKGTWN---LNTLLPKLIDFVVDK-GVDILPLASSENFGKDKFFWFRDEEFARQPLAG 437
           V   G      L++++P+L++ + D  G  IL   +  N  KDKF W RDEEFAR+ LAG
Sbjct: 396 VELPGVEKLGFLHSIVPRLLELLRDSPGDKILLFDTPANVQKDKFAWLRDEEFARETLAG 455

Query: 438 LNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE------------- 497
           +NPY+I LV+E+PLKSKL+PA+YG  ESAI+ +++E+Q++ +MTV E             
Sbjct: 456 INPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFH 515

Query: 498 ---------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWD 557
                    +R LK  T+YGSRT+FFL  D TLR LAIEL+ P    QPQW+ VFTP  D
Sbjct: 516 DLFLPYVHKIRSLKHTTMYGSRTIFFLTDDGTLRLLAIELTRPASPSQPQWRQVFTPSTD 575

Query: 558 ATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHF 617
            T  WLWR+AKAHV AHD GHH+L++HWLRTHC+VEPYIIAA+RQLS MHPIY+LLHPHF
Sbjct: 576 TTKSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLHPHF 635

Query: 618 RYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLA 677
           RYTM  NA+AR  LI+  GIIE +FSP KYSME SSVAY K W+F++EALPADL+ RG+A
Sbjct: 636 RYTMRINALARSRLISAAGIIELSFSPQKYSMELSSVAYDKLWRFDMEALPADLVRRGMA 695

Query: 678 VEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTE 737
            EDP A HGL+L IEDYP+ANDGL++WDAIK W   YV  +YPD   V  D+ELQA+WTE
Sbjct: 696 EEDPTAEHGLRLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQAFWTE 755

Query: 738 IRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIA 797
           +RT GHGDKKD PWW  L++PE L   +TTI+WV + HHA VNFGQY F  YFPNRPSIA
Sbjct: 756 VRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIA 815

Query: 798 RVNMPTEEPM--KLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDI 830
           R  MP EEP+     + FL+ P+    + FP+Q QAT+V +V+++LS+HS+DE+YLG + 
Sbjct: 816 RTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSTHSTDEEYLGGEQ 875

BLAST of Moc02g00970 vs. ExPASy TrEMBL
Match: A0A6J1H492 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459464 PE=3 SV=1)

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 623/905 (68.84%), Postives = 694/905 (76.69%), Query Frame = 0

Query: 1   MLRSPSYLSPSSSSSNKALLVSGKPFVALVGNYEEALFRIEQRVKPNAAPGPAFGLRFAS 60
           MLRSP Y   S SSS +  LV G+P+                 +KP          R A 
Sbjct: 1   MLRSPVYY--SLSSSYQVHLVLGRPY----------------GIKPYTTL-----TRCAP 60

Query: 61  PEYSVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVL 120
           P  SV+KAS  A  + P+SAKAVVTV RP+       +NFG+K+RL DIT LWG KTLVL
Sbjct: 61  P--SVVKASVDAPMDHPLSAKAVVTVHRPLRG---LSLNFGIKQRLHDITDLWGAKTLVL 120

Query: 121 EILSAEIDPTTVLEKGTIKGYGQKVNKEDD-------------------------EHNEE 180
           E+LSAEID  T LEKGTIKGY ++V+++ D                         EHN+E
Sbjct: 121 ELLSAEIDSRTGLEKGTIKGYAERVDRDGDDVITYETKFKVPAEFGSIGAVLVENEHNQE 180

Query: 181 LFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT--------------------- 240
           LFL+DIV+ G+PT PLRFSCNSWL  KSQSDD RIFFTT                     
Sbjct: 181 LFLKDIVVYGIPTAPLRFSCNSWLTPKSQSDDTRIFFTTKSYLPSNTPKGLKRIRAEELK 240

Query: 241 --------KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSD 300
                   KRE HHRIYDYDVYNDLG+ DKG EF+RPVLGGKQ+PYPRRCRTGRAR ++D
Sbjct: 241 KLQGNGYGKRETHHRIYDYDVYNDLGDPDKGVEFQRPVLGGKQHPYPRRCRTGRARCQTD 300

Query: 301 PLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTD 360
            LSEVRS D+YLPRDEAF RI+ V LL  TFYS++N LK  LET + DTK  +    +  
Sbjct: 301 SLSEVRSWDIYLPRDEAFSRIEYVRLLGNTFYSLLNSLK--LETTITDTKFSITEELSNS 360

Query: 361 DVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNP 420
           +   KG WN N +LPKL   VV K VDIL L SSE F KDKFFWFRDEEFARQ LAGLNP
Sbjct: 361 NKGIKGIWNFNAILPKLFSSVVTKAVDILTLPSSETFDKDKFFWFRDEEFARQTLAGLNP 420

Query: 421 YSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE---------------- 480
           YSIRLVKEWPLKS+LEP IYG PESAI+DEIVE+QIKG+MT+ E                
Sbjct: 421 YSIRLVKEWPLKSQLEPDIYGPPESAITDEIVERQIKGVMTLEEAVKQKKLYILDYHDLL 480

Query: 481 ------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATG 540
                 VRKLKGKTLYGSRTLFFLNPD TLRPLAIELS PP+DG+ QWKDV TPCWDATG
Sbjct: 481 VPYVAKVRKLKGKTLYGSRTLFFLNPDGTLRPLAIELSRPPMDGKAQWKDVLTPCWDATG 540

Query: 541 LWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYT 600
           LWLW+I KAHVLAHD+GHHQLVSHWLRTHC VEPYIIA HRQLSAMHPIYRLLHPHFRYT
Sbjct: 541 LWLWKIGKAHVLAHDSGHHQLVSHWLRTHCCVEPYIIATHRQLSAMHPIYRLLHPHFRYT 600

Query: 601 METNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVED 660
           METNAIAR +LINEGGIIETTFSPGKYSMEFSS+AY + WQFNLEALPADLIHRGLAVED
Sbjct: 601 METNAIARSSLINEGGIIETTFSPGKYSMEFSSLAYRQHWQFNLEALPADLIHRGLAVED 660

Query: 661 PNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRT 720
           PNAPHGLKL+IEDYPYANDGLILWDAIKEWA EYVNHYYPDPSVVKSD+ELQAWWTEIR 
Sbjct: 661 PNAPHGLKLSIEDYPYANDGLILWDAIKEWAMEYVNHYYPDPSVVKSDEELQAWWTEIRE 720

Query: 721 VGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVN 780
           VGHGDKK+EPWW VL+TP+DLID+VTTIMW+T+GHHAV NFGQYSFAKYFPNRPSIAR N
Sbjct: 721 VGHGDKKEEPWWPVLDTPKDLIDIVTTIMWITTGHHAVANFGQYSFAKYFPNRPSIARAN 780

Query: 781 MPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWA 830
           +PTE+P K WKYFLEKPENVFL+TFPTQ QATIVSSV ++L+SH  DEKYLGKD+E AWA
Sbjct: 781 VPTEQPKKHWKYFLEKPENVFLETFPTQIQATIVSSVASVLASHPPDEKYLGKDMEAAWA 840

BLAST of Moc02g00970 vs. ExPASy TrEMBL
Match: A0A6J1D4V5 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017327 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 599/909 (65.90%), Postives = 683/909 (75.14%), Query Frame = 0

Query: 1   MLRSPSYLSPSSSSSNKALLVSGKPFVALVGNYEEALFRI-EQRVKPNAAPGPAFGLRFA 60
           ML+SP Y      +S +AL +   PF+      E  L  I  Q VKPNAAP     L F 
Sbjct: 1   MLKSPVY------NSKQALCIYRGPFLLSNNVAEPNLLPIGRQSVKPNAAP----RLCFD 60

Query: 61  SPEYSVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL- 120
           SP  +V  ++A  TGN PVSA  VVT+KRP      FP        +  ITH W TKT  
Sbjct: 61  SPNSAVKSSAATPTGN-PVSAAVVVTLKRPP-----FP-------SIDGITHFWATKTKP 120

Query: 121 -VLEILSAEIDPTTVLEKGTIKGYGQKVNKED-------------------------DEH 180
            VLE+LSA++DPTT LEKGTIK Y +KV +ED                         ++H
Sbjct: 121 PVLELLSADLDPTTGLEKGTIKVYAKKVAREDAEVIMYDIKFVIPADFGAIGAVLVENQH 180

Query: 181 NEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT------------------ 240
           N+ELFL+DIVIQGLPTGP+RFSCNSWL+ KS S DRRIFFTT                  
Sbjct: 181 NKELFLKDIVIQGLPTGPVRFSCNSWLSPKSHSGDRRIFFTTKSYLPSNTPDGLKRFRAK 240

Query: 241 -----------KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARY 300
                      KREAHHRIYDYDVYNDLG+ D GDE+KRPVLGGKQ+PYPRRCRTGR+R 
Sbjct: 241 ELRNLQDNGSGKREAHHRIYDYDVYNDLGDPDNGDEYKRPVLGGKQHPYPRRCRTGRSRS 300

Query: 301 RSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLS 360
            +DPLSEVRSL+VY+PRDE FL IKQV +LAK    M++ + P LE    DT L   HLS
Sbjct: 301 PTDPLSEVRSLNVYVPRDETFLEIKQVNMLAKAVSWMLHCVVPTLEA---DTNLCSSHLS 360

Query: 361 TTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAG 420
            T+++ +K TWNLNTLL +L+DFV  K VD+LP + S+ F  DKFFWFRDEEFARQ LAG
Sbjct: 361 NTNNL-SKHTWNLNTLLRRLVDFVAHKVVDVLP-SPSQTFNGDKFFWFRDEEFARQTLAG 420

Query: 421 LNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE------------- 480
           LNPYSIRLV EWP+KSKL+P IYG PESAI+DEIVE+QIKG MT++E             
Sbjct: 421 LNPYSIRLVTEWPIKSKLDPCIYGPPESAITDEIVEEQIKGFMTLDEAVKQKKLFALDYH 480

Query: 481 ---------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWD 540
                    VRKLKGKTLYGSRTLFFLNPDNTL+PLAIEL+ PP + +PQWKDVF PC D
Sbjct: 481 DLFLPYVTKVRKLKGKTLYGSRTLFFLNPDNTLKPLAIELTRPPTNAKPQWKDVFKPCCD 540

Query: 541 ATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHF 600
           ATGLWLWRIAKAHV+AHD G+HQLVSHWLRTHCSVEPYIIA HRQLSAMHPIYRLLHPHF
Sbjct: 541 ATGLWLWRIAKAHVVAHDFGYHQLVSHWLRTHCSVEPYIIATHRQLSAMHPIYRLLHPHF 600

Query: 601 RYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLA 660
           R T+E NA AR+TLIN  GIIET FSPGKYSME +SV Y KQWQFNLEALPADLI+RGLA
Sbjct: 601 RGTIEINAAARKTLINVEGIIETAFSPGKYSMEITSVVYQKQWQFNLEALPADLINRGLA 660

Query: 661 VEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTE 720
           VEDPNAPHGLKLTIEDYP+AND LILW+AIK+WATEYVNHYYP+P+++K D+ELQAWW E
Sbjct: 661 VEDPNAPHGLKLTIEDYPFANDALILWEAIKQWATEYVNHYYPEPNLIKLDEELQAWWME 720

Query: 721 IRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIA 780
           IRTVGHGDKKDEPWW +LNTP+DLIDVVTTIMWVTSGHHA VNFGQY F +YFP RPS A
Sbjct: 721 IRTVGHGDKKDEPWWPILNTPKDLIDVVTTIMWVTSGHHAAVNFGQYFFTRYFPIRPSFA 780

Query: 781 RVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIE 830
           RV++PTE+P  +LWKYFLE PE VF DTFPT  +A+++  ++NILS+H  DEKYLG ++E
Sbjct: 781 RVDVPTEDPNSRLWKYFLENPEKVFSDTFPTHVEASVLLCILNILSTHPPDEKYLGGEME 840

BLAST of Moc02g00970 vs. ExPASy TrEMBL
Match: A0A6J1D4V6 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017309 PE=3 SV=1)

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 567/836 (67.82%), Postives = 649/836 (77.63%), Query Frame = 0

Query: 77  PVSAKAVVTVKRPVGNGELFPINFGLKRRLPDIT-HLWGTKTLVLEILSAEIDPTTVLEK 136
           P SA  VVTVKR V  G L   N GLKR L D+T +LWGTKTLV E+LS E+DPTT LEK
Sbjct: 4   PFSAHVVVTVKRHV--GRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPTTGLEK 63

Query: 137 GTIKGYGQKVNKEDD---------------------------EHNEELFLEDIVIQGLPT 196
           GTIKGY QKV +E++                           ++N ELFL+DIVI G+P 
Sbjct: 64  GTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGAVLVENQYNTELFLKDIVIHGVPY 123

Query: 197 GPLRFSCNSWLNSKSQSDDRRIFFTT-----------------------------KREAH 256
           GPLRFSCNSWL+ KS S DRRIFFTT                             KRE+H
Sbjct: 124 GPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEGLKRFREEDLKILQGNGHGKRESH 183

Query: 257 HRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLP 316
           HRIYDYDVYNDLG+ D+GDEFKRP+LGGKQ+PYPRRCRTGRAR ++DPLSEV S + Y+P
Sbjct: 184 HRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRCRTGRARSQADPLSEVTSSNFYVP 243

Query: 317 RDEAFLRIKQVTLLA-KTFYSMINGLKPALETIVPDTKLHVPHLSTTDDVP-NKGTWNLN 376
           RDEAF+  KQVT  A KT YSM++G  PALE I  D +    +      +P +K TW  +
Sbjct: 244 RDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDIESRFTNRVML--LPSSKYTWEPS 303

Query: 377 TLLPKLIDFVVDKGVDILPLASSEN-FGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWP 436
            LLP+LIDF VDK V IL L  S+     DKFFWF+DEEFARQ LAGLNP SIRLV EWP
Sbjct: 304 ALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKDEEFARQTLAGLNPCSIRLVTEWP 363

Query: 437 LKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE----------------------VRKL 496
           L+SKL+PAIYG  +SAI++EIVEQQIKG MT+NE                      VRK+
Sbjct: 364 LESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNEAVEQKKLYILDYHDLFLPYVAKVRKI 423

Query: 497 KGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATGLWLWRIAKAH 556
           +GKTLYGSRTLFFLNPDNTL+PLAIEL+ PPIDG+ QWKDVFTPC ++TGLWLWRIAKAH
Sbjct: 424 EGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQWKDVFTPCLNSTGLWLWRIAKAH 483

Query: 557 VLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQT 616
           VLAHD+G+HQLVSHWLRTHCSVEPYIIA HRQLSAMHPIYRLLHPHFRYTME NAIAR+ 
Sbjct: 484 VLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMHPIYRLLHPHFRYTMEINAIAREA 543

Query: 617 LINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLT 676
           L N GGIIET FS GKYSME SS+ Y KQWQFNLEALPADLIHRG+AVEDPNA HG+KL+
Sbjct: 544 LTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEALPADLIHRGMAVEDPNAAHGVKLS 603

Query: 677 IEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEP 736
           IEDYP+ANDGLILWDAIKEWATEYVNHYYPDP VVKSD ELQAWWTEIR+VGHGDKK+EP
Sbjct: 604 IEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKSDKELQAWWTEIRSVGHGDKKEEP 663

Query: 737 WWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKL 796
           WW +LNTPEDLIDVV+++MWVTSGHHA VNFGQY FA YFP RPS+AR+N+PTEEP  KL
Sbjct: 664 WWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFASYFPARPSVARINVPTEEPNSKL 723

Query: 797 WKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWADEPHIKRAF 830
           WK FLE PE VFLDTFP   QAT++ S+++ILS+HS +E+YLG+D+EPAW D+P +K+AF
Sbjct: 724 WKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSPEEEYLGEDMEPAWGDDPVVKQAF 783

BLAST of Moc02g00970 vs. ExPASy TrEMBL
Match: A0A6J1FWY9 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448227 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 555/851 (65.22%), Postives = 646/851 (75.91%), Query Frame = 0

Query: 64  SVIKASAVATG-NKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEI 123
           +VIKAS+VA+   KP+S KA+VTV+R +G+G        L+R L DI  L+G K+LV+E+
Sbjct: 59  AVIKASSVASSTEKPLSVKALVTVQRTLGSG------LYLERGLDDIADLFG-KSLVVEL 118

Query: 124 LSAEIDPTTVLEKGTIKGYGQKVNKEDD------------------------EHNEELFL 183
           +SAE+DP T LEK TI+ Y  K + E D                        EH++E+++
Sbjct: 119 VSAEVDPATGLEKATIRHYAHKEDTEGDEITYEADFKIPPDFGIIGAIFVENEHHKEMYV 178

Query: 184 EDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT------------------------ 243
           +DIV++G P GPL F C+SW+N K   D +R+FFTT                        
Sbjct: 179 KDIVLEGHPIGPLHFVCSSWVNEKDLPDSKRVFFTTKSYLPSNTPEGLKKLREEELKAVR 238

Query: 244 -----KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLS 303
                KRE+H RIYDYDVYNDLG+ +K  E KRPVLGGKQ+PYPRRCRTGR R   DP+S
Sbjct: 239 GNGVGKRESHERIYDYDVYNDLGDPNKNPEHKRPVLGGKQFPYPRRCRTGRPRASRDPVS 298

Query: 304 EVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDV- 363
           E RSL+ Y+PRDEAF  +KQ T  A +  S++NGL PALE++  D  +  PH    DD+ 
Sbjct: 299 ESRSLNNYVPRDEAFSALKQATFNASSLNSVLNGLIPALESVSTDNDIRFPHFPAIDDLF 358

Query: 364 -------PNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPL 423
                  P+KG W L TLLP++ID VVD   DIL     E F +DKFFWFRDEEF RQ L
Sbjct: 359 DNGVPLPPSKGGWKLATLLPRIIDTVVDSAEDILRFHPPETFDRDKFFWFRDEEFGRQTL 418

Query: 424 AGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNE----------- 483
           AGLNPYS+RLV EWPLKSKL+PA+YG PES I+ +I+EQQI+G MT++E           
Sbjct: 419 AGLNPYSLRLVTEWPLKSKLDPAVYGPPESEITGKIIEQQIRGFMTLDEALTKKRLFILD 478

Query: 484 -----------VRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPC 543
                      VRKLKG TLYGSRTLFFL+ D+TL+PLAIEL+ PPIDG+PQWK VFTP 
Sbjct: 479 YHDLLLPYVAKVRKLKGTTLYGSRTLFFLHSDSTLKPLAIELTRPPIDGKPQWKKVFTPF 538

Query: 544 WDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHP 603
           WDAT +WLWRIAKAHVLAHD+G+HQLVSHWLRTHC VEPYIIA++RQLSAMHPIYRLLHP
Sbjct: 539 WDATSVWLWRIAKAHVLAHDSGYHQLVSHWLRTHCCVEPYIIASNRQLSAMHPIYRLLHP 598

Query: 604 HFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRG 663
           HFRYTME NA+ARQ LIN  GIIET+FSPGKYSMEFSSVAY +QWQFNLEALPADLIHRG
Sbjct: 599 HFRYTMEINALARQALINANGIIETSFSPGKYSMEFSSVAYKEQWQFNLEALPADLIHRG 658

Query: 664 LAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWW 723
           LAVEDPNAPHGLKLTIEDYP+ANDGLILWDAIK+WATEYVN+YYPDPS+VKSD+ELQAWW
Sbjct: 659 LAVEDPNAPHGLKLTIEDYPFANDGLILWDAIKDWATEYVNYYYPDPSLVKSDEELQAWW 718

Query: 724 TEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPS 783
           TEIRT GH DKKDEPWW VLNTPEDLI VVTTIMWVTSGHHA VNFGQYSFA YFPNRPS
Sbjct: 719 TEIRTEGHADKKDEPWWPVLNTPEDLISVVTTIMWVTSGHHAAVNFGQYSFAGYFPNRPS 778

Query: 784 IARVNMPTEEPMK-LWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKD 830
           IAR+N+P EE  K  W+YFL KPE+V L+TFPTQ QAT V++V+NILSSHS DE+Y+GKD
Sbjct: 779 IARINVPVEEKNKEKWEYFLNKPEDVLLETFPTQLQATKVTAVLNILSSHSPDEEYMGKD 838

BLAST of Moc02g00970 vs. ExPASy TrEMBL
Match: A0A6J1H3S4 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459733 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 570/883 (64.55%), Postives = 650/883 (73.61%), Query Frame = 0

Query: 29  LVGNYEEALFRIEQRVKPNAAPGPAFGLRFASPEYSVIKASAVATG----NKPVSAKAVV 88
           L GN +  +        P A+P     LRF SP  +VIK SAV         PV  KAVV
Sbjct: 25  LPGNVDACVSLQHSLTPPAASP-----LRFTSP--NVIKPSAVHVSVPSIQSPVYTKAVV 84

Query: 89  -TVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEILSAEIDPTTVLEKGTIKGYGQ 148
            TV+RP+   +LFP  FG   RL DI +   ++ L LE+LSA++DPTT LEKG +K Y Q
Sbjct: 85  TTVQRPI--HQLFP-KFGFIGRLHDIINR-RSRVLALELLSADMDPTTGLEKGRMKAYEQ 144

Query: 149 KVNKEDDE------------------------HNEELFLEDIVIQGLPTGPLRFSCNSWL 208
           KV +EDDE                        H +E+FL+DIVI G+PTGPL FSCNSW+
Sbjct: 145 KVKREDDEIIYEISFVIPADFGPIGAVLVENMHKKEVFLKDIVIHGIPTGPLHFSCNSWI 204

Query: 209 NSKSQSDDRRIFFTT-----------------------------KREAHHRIYDYDVYND 268
            SK+Q +DRRIFFTT                             KRE   RIYDYDVYND
Sbjct: 205 TSKAQCNDRRIFFTTKSYLPSKTPDGLKRLRLEELRILQGNGYGKREKQERIYDYDVYND 264

Query: 269 LGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAF-LRIKQ 328
           LGN DKGDE+KRPVLGGKQ+PYPRRCRTGR R ++DP SEV+SL+ Y+PRDEAF + + Q
Sbjct: 265 LGNPDKGDEYKRPVLGGKQHPYPRRCRTGRPRTQTDPSSEVKSLEFYVPRDEAFSIPMIQ 324

Query: 329 VTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVD 388
              L K  +SM++ L PAL+TI  D +  +          +K TW  N+LLP L+     
Sbjct: 325 ENPLTKALHSMLSALLPALDTIGTDKQCSL----------SKHTWRFNSLLPTLM----- 384

Query: 389 KGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSP 448
            G+ +L  + + N G DKFFWF+DEEFARQ LAGLNPYSIRLV EWPL+SKLEP+IYGSP
Sbjct: 385 -GILLLDSSQTSNNG-DKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLESKLEPSIYGSP 444

Query: 449 ESAISDEIVEQQIKGIMTVNE----------------------VRKLKGKTLYGSRTLFF 508
           ESAI+DEIVEQQIKG MT++E                      VR+LKG+TLYGSRTLFF
Sbjct: 445 ESAITDEIVEQQIKGFMTIDEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFF 504

Query: 509 LNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVS 568
           LNPDNTLRPLAIELS PPID +PQWKDVFTPCWDA GLWLWRIAKAHVLAHD+GHHQLVS
Sbjct: 505 LNPDNTLRPLAIELSRPPIDDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGHHQLVS 564

Query: 569 HWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFS 628
           HWLRTHC VEPY+IA HRQLSAMHPIYRLLHPHFR+TM  NA+AR+TLIN  G++E+ FS
Sbjct: 565 HWLRTHCCVEPYVIATHRQLSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLVESIFS 624

Query: 629 PGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLIL 688
           PGKYSME SSV Y KQWQFNLEALPADLIHRGLAVEDPNAPHGLKL+IEDYP+ANDGLIL
Sbjct: 625 PGKYSMEISSVVYEKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLIL 684

Query: 689 WDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLID 748
           WDAIK+W TEYVNHYYPDPS+V SD ELQAWWTEIR VGHGDKKDEPWW +LNTP DLI 
Sbjct: 685 WDAIKQWVTEYVNHYYPDPSLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIH 744

Query: 749 VVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFL 808
           +VTTI+WVTSGHHA VNFGQY F  Y+P RPS+ R+N+PTEEP   L KYFLE PENVFL
Sbjct: 745 IVTTIIWVTSGHHAAVNFGQYPFTSYYPIRPSLTRLNIPTEEPNSTLRKYFLENPENVFL 804

Query: 809 DTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEI 830
           DTFPTQ QA I+  ++NILSSHS DE+YLG D+EPAW DEP IK AFEKFS KLKKLEEI
Sbjct: 805 DTFPTQIQANILLFILNILSSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEI 864

BLAST of Moc02g00970 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 805.8 bits (2080), Expect = 3.2e-233
Identity = 418/839 (49.82%), Postives = 534/839 (63.65%), Query Frame = 0

Query: 85  TVKRPVGN---------GELFPINFGLKRRLPDITHLWGTKTLVLEILSAEIDPTTVLEK 144
           TVK P+ N          E F       R L DI  + G ++L++E++SA+ D     ++
Sbjct: 65  TVKEPIQNIKVKGYITAQEEFLEGITWSRGLDDIADIRG-RSLLVELISAKTD-----QR 124

Query: 145 GTIKGYGQKVNKE------------------------DDEHNEELFLEDIVIQGLPTGPL 204
            T++ Y Q+V  E                         ++++ +LFL+ + ++ LP G +
Sbjct: 125 ITVEDYAQRVWAEAPDEKYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSI 184

Query: 205 RFSCNSWLNSKSQSDDRRIFFTTK--------------------------RE------AH 264
            F+C SW+  KS    +RIFF+ K                          RE        
Sbjct: 185 TFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKF 244

Query: 265 HRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVR-SLDVYL 324
            RIYDYDVYND+G+ D   E  RPV+GG  +PYPRRC+TGR    +DP SE R   + Y+
Sbjct: 245 ERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYV 304

Query: 325 PRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLSTTDDVPNKGTW---- 384
           PRDE F   K  +   K   + +  + P +E+++   +   PH     ++  +G      
Sbjct: 305 PRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKD 364

Query: 385 -NLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVK 444
             L  LLP++I  + +   DIL   +     +D+F W RD+EFARQ LAGLNPYSI+LV+
Sbjct: 365 AGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVE 424

Query: 445 EWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMT----------------------VNEV 504
           EWPL SKL+PA+YG P S I+ EIVE+++KG MT                      VN+V
Sbjct: 425 EWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKV 484

Query: 505 RKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWKDVFTPCWDATGLWLWRIA 564
           R+L   TLY SRTLFFL+ D+TLRP+AIEL+ PP   +PQWK VFTP +DAT  WLW +A
Sbjct: 485 RELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWNLA 544

Query: 565 KAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIA 624
           K H ++HD G+HQL+SHWLRTH   EPYIIAA+RQLSAMHPIYRLLHPHFRYTME NA A
Sbjct: 545 KTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARA 604

Query: 625 RQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGL 684
           RQ+L+N GGIIET F PGKY++E SS  Y K W+F+ E LPADLI RGLA ED  A HG+
Sbjct: 605 RQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGV 664

Query: 685 KLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKK 744
           +LTI DYP+ANDGLILWDAIKEW T+YV HYYPD  ++ SD+ELQ WW+E+R +GHGDKK
Sbjct: 665 RLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKK 724

Query: 745 DEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPM 804
           DEPWW VL T +DLI VVTTI WVTSGHHA VNFGQY +  YFPNRP+  R+ MPTE+P 
Sbjct: 725 DEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPT 784

Query: 805 -KLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSSHSSDEKYLGKDIEPAWADEPHIK 830
            +  K F E PE V L T+P+Q QAT+V   +++LS+HS DE+Y+G+  E +WA+EP I 
Sbjct: 785 DEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVIN 844

BLAST of Moc02g00970 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 632.5 bits (1630), Expect = 4.8e-181
Identity = 336/810 (41.48%), Postives = 477/810 (58.89%), Query Frame = 0

Query: 116 KTLVLEILSAEIDPTTVLEK----GTIKGYGQKVNKEDD--------------------- 175
           + +VLE++S ++DP T L K      +K + +K   + +                     
Sbjct: 117 RNIVLELISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPGAIT 176

Query: 176 ---EHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTK------------ 235
              +H +E FLE I I+G   GP+ F CNSW+ S+    D+RIFFT +            
Sbjct: 177 VMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPSGLR 236

Query: 236 -----------------REAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRT 295
                            R+   RIYD+DVYNDLGN DK  E  RP LGGK+ PYPRRCRT
Sbjct: 237 VLREKELKNLRGDGSGVRKLSDRIYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRRCRT 296

Query: 296 GRARYRSDPLSEVR---SLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALE-TIVPD 355
           GR    SD  +E R    L +Y+PRDE F   KQ T  A    ++++ L P+L+ +IV +
Sbjct: 297 GRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAE 356

Query: 356 --------TKLH----VPHLSTTDDVPNKGTWNLNTLLPK-LIDFVVDKGVDILPLASSE 415
                    +L+    +  L   DD+  K        LPK ++D + +    +L   + +
Sbjct: 357 DFADFGEIDRLYKEGLLLKLGFQDDIFKKFP------LPKVVVDTLQESTKGLLKYDTPK 416

Query: 416 NFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQ 475
              KDK  W RD+EFARQ +AG+NP +I  VK +P  S L+P IYG   SA++D+ +   
Sbjct: 417 ILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGH 476

Query: 476 IKGIMT---------------------VNEVRKLKGKTLYGSRTLFFLNPDNTLRPLAIE 535
           + G                        ++ +  L G+  Y +RT+FFL    TL+P+AIE
Sbjct: 477 LDGFSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIE 536

Query: 536 LSAPPIDGQPQWKDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYI 595
           LS PP   + + K V TP  DAT  W+W++AKAHV ++D G HQLV+HWLRTH  +EP+I
Sbjct: 537 LSLPPHGPKHRSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFI 596

Query: 596 IAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAY 655
           +AAHRQLSAMHPI++LL PH RYT+E NA+ARQ+LI+  G+IE  F+ G Y ME S+ AY
Sbjct: 597 LAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSAAAY 656

Query: 656 AKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVN 715
              W+F++E LPADLI RG+A+ D   PHGLKL IEDYPYANDGL+LW AI+ W   YV 
Sbjct: 657 KSSWRFDMEGLPADLIRRGMAIPDATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRTYVE 716

Query: 716 HYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHH 775
            YYP+P+++K+D ELQ+W++E   VGH D +D  WW  L+T +DL+ ++TT++W+ S  H
Sbjct: 717 RYYPNPNLIKTDSELQSWYSESINVGHADLRDADWWPELSTVDDLVSILTTLIWLASAQH 776

Query: 776 AVVNFGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVSS 830
           A +NFGQY +  Y PNRP + R  +P E   + +  F+  PE  +  + P+  Q +   +
Sbjct: 777 AALNFGQYPYGGYVPNRPPLMRRLIPDESDPE-YASFISHPEKYYFSSMPSLAQTSKFMA 836

BLAST of Moc02g00970 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 623.2 bits (1606), Expect = 2.9e-178
Identity = 331/811 (40.81%), Postives = 474/811 (58.45%), Query Frame = 0

Query: 116 KTLVLEILSAEIDPTTVLEK----GTIKGYGQKVNKE----------------------- 175
           + +VLE++S ++DP T   K      +K + +K N +                       
Sbjct: 123 RNVVLELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAEFTVDSAFGSPGAIT 182

Query: 176 -DDEHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT------------- 235
             ++H +E FLE I I+G   GP+ F CNSW+ S+     +RI FT              
Sbjct: 183 VTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSETPSGLR 242

Query: 236 ----------------KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRT 295
                           +R+   RIYDYDVYND+GN D   E  RP LGG+++PYPRRCRT
Sbjct: 243 TLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISRELARPTLGGREFPYPRRCRT 302

Query: 296 GRARYRSDPLSEVR---SLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALE-TIVPD 355
           GR+   +D +SE R    L +Y+PRDE F   KQ T  A    ++++ L P+L+ +I+ +
Sbjct: 303 GRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKASILAE 362

Query: 356 TKLHVPHLST------------TDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSEN 415
              +   + +             DD+  K        LPK++  +      +L   + + 
Sbjct: 363 DFANFGEIDSLYKEGLLLKLGFQDDMFKKFP------LPKIVTTLQKSSEGLLRYDTPKI 422

Query: 416 FGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYG-SPESAISDEIVEQQ 475
             KDK+ W RD+EFARQ +AG+NP +I  V  +P  S L+P IYG    SA++++ +  Q
Sbjct: 423 VSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQ 482

Query: 476 IKGIMT---------------------VNEVRKLKGKTLYGSRTLFFLNPDNTLRPLAIE 535
           + G+                       ++ +  L G+  Y +RT+ FL    TL+P+AIE
Sbjct: 483 LDGLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIE 542

Query: 536 LSAPPIDGQPQ-WKDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPY 595
           LS P      Q  K V TP  DAT  W+W++AKAHV ++D G HQLV+HWLRTH  +EP+
Sbjct: 543 LSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPF 602

Query: 596 IIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVA 655
           I+AAHRQLSAMHPI++LL PH RYT+E NA+ARQTLI+  G+IE+ F+ G+Y +E SS A
Sbjct: 603 ILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAGQYGLEISSAA 662

Query: 656 YAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYV 715
           Y  +W+F++E LPADLI RG+AV DP  PHGLKL +EDYPYANDGL+LW AI+ W   YV
Sbjct: 663 YKNKWRFDMEGLPADLIRRGMAVPDPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYV 722

Query: 716 NHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGH 775
             YY + +++++D ELQAW++E   VGH D +D  WW  L+T EDL+ V+TTI+W+ S  
Sbjct: 723 ERYYANSNLIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQ 782

Query: 776 HAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVS 830
           HA +NFGQY +  Y PNRP + R  +P E   + +  F+E P+  F  + P+  Q T   
Sbjct: 783 HAALNFGQYPYGGYVPNRPPLMRRLIPDESDPE-FTSFIEDPQKYFFSSMPSLLQTTKFM 842

BLAST of Moc02g00970 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 612.8 bits (1579), Expect = 4.0e-175
Identity = 322/744 (43.28%), Postives = 449/744 (60.35%), Query Frame = 0

Query: 155 ELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT-------------------- 214
           E+ L +I+I+   T  + F  N+W++SK+ +   RI F +                    
Sbjct: 179 EICLSEIIIED-STDTILFPANTWIHSKNDNPQARIIFRSQPCLPSETPDGIKELREKDL 238

Query: 215 ---------KREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRS 274
                    +R+ H RIYDYDVYNDLG+  K  E  RPVLG  + PYPRRCRTGR     
Sbjct: 239 VSVRGDGKGERKPHERIYDYDVYNDLGDPRK-TERVRPVLGVPETPYPRRCRTGRPLVSK 298

Query: 275 DPLSEVRSL---DVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHL 334
           DP  E R     + Y+PRDE F  IK+ T  A  F ++ + L P++   + +  +     
Sbjct: 299 DPPCESRGKEKEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSIAAALSNLDIPFTCF 358

Query: 335 STTDDVPNKG---------TWNLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRD 394
           S  D++                L   +   ++ +++    +L   +      D+F W RD
Sbjct: 359 SDIDNLYKSNIVLGHTEPKDTGLGGFIGGFMNGILNVTETLLKYDTPAVIKWDRFAWLRD 418

Query: 395 EEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMT------ 454
            EF RQ LAG+NP +I L+KE P++S L+PA+YG  ES +++EI+ ++++   T      
Sbjct: 419 NEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVEHYGTTIEKAL 478

Query: 455 --------------------VNEVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPID 514
                               +N +++   KT Y SRT+FF + +  LRPLAIELS PP  
Sbjct: 479 EEKRLFLVDYHDILLPFVEKINSIKEDPRKT-YASRTIFFYSKNGALRPLAIELSLPP-T 538

Query: 515 GQPQWKDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQL 574
            + + K V+T   DAT  W+W++AKAHV ++D G HQLV+HWLRTH S+EPYIIA +RQL
Sbjct: 539 AESENKFVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIATNRQL 598

Query: 575 SAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFN 634
           S MHP+Y+LLHPH RYT+E NA AR++LIN GGIIE+ F+PGKY+ME SS AY   W+F+
Sbjct: 599 STMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRFD 658

Query: 635 LEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPS 694
           +E LPADL+ RG+A ED +A  G++L I+DYPYA DGL++W AIK+    YV H+Y D  
Sbjct: 659 MEGLPADLVRRGMAEEDSSAECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHFYSDSK 718

Query: 695 VVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQ 754
            + SD ELQAWW EI+  GH DKKDEPWW  LNT +DL  ++T ++W+ SG HA +NFGQ
Sbjct: 719 SITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFGQ 778

Query: 755 YSFAKYFPNRPSIARVNMPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVSSVMNILSS 814
           Y F  Y PNRP++ R  +P E     ++ F+  P+  FL + PTQ QAT V +V   LS+
Sbjct: 779 YPFGGYVPNRPTLLRKLIPQETDPD-YEMFMRNPQYSFLGSLPTQLQATKVMAVQETLST 838

Query: 815 HSSDEKYL--GKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGVE 830
           HS DE+YL   ++++  W  +  + + F KFS +L K+E+ I+ERN + KLKNR GAG+ 
Sbjct: 839 HSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRTGAGMP 898

BLAST of Moc02g00970 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 566.2 bits (1458), Expect = 4.2e-161
Identity = 332/801 (41.45%), Postives = 441/801 (55.06%), Query Frame = 0

Query: 101 GLKRRLPDITHLWGTKTLVLEILSAEIDPTTVLEKGTIKGY-GQKVNKEDDEHNEELFLE 160
           G K +L    HL    T +  + + E       +  T  GY G  + +  + H  E  L+
Sbjct: 69  GSKGKLGKAAHLEDWITTITSLTAGESAFKVTFDYETDFGYPGAFLIR--NSHFSEFLLK 128

Query: 161 DIVIQGLP-TGPLRFSCNSWLNSKSQSDDRRIFFTTKREAHH------------------ 220
            + ++ +P  G + + CNSW+         R+FF+ K    H                  
Sbjct: 129 SLTLEDVPGHGRVHYICNSWIYPAKHYTTDRVFFSNKTYLPHETPATLLKYREEELVSLR 188

Query: 221 -----------RIYDYDVYNDLGNSDKGDEFKRPVLGGKQ-YPYPRRCRTGRARYRSDPL 280
                      R+YDY  YNDLG   K     RPVLGG Q YPYPRR RTGR   + DP 
Sbjct: 189 GTGEGELKEWDRVYDYAYYNDLGVPPKN---PRPVLGGTQEYPYPRRGRTGRKPTKEDPQ 248

Query: 281 SEVR-----SLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALETIVPDTKLHVPHLS 340
           +E R     SLD+Y+PRDE F  +K    LA    ++   ++PALE +  DT        
Sbjct: 249 TESRLPITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSFE 308

Query: 341 TT-------DDVPNKGTWN---LNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRD 400
                     D+PN+   +    N  L  L +     G   L     +   +DK  W  D
Sbjct: 309 DVLKIYEEGIDLPNQALIDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTD 368

Query: 401 EEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAISDEIVEQQIKGIMTVNEVRK 460
           EEFAR+ LAGLNP  I+L+KE+P KSKL+   YG+  S I+   +E  + G+     + K
Sbjct: 369 EEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLDGLTVEEALEK 428

Query: 461 LK--------------GKT------LYGSRTLFFLNPDNTLRPLAIELSAPPIDGQP--Q 520
            +              G+        Y SRTL FL  D TL+PL IELS P  +G     
Sbjct: 429 ERLFILDHHDTLMPYLGRVNTTTTKTYASRTLLFLKDDGTLKPLVIELSLPHPNGDKFGA 488

Query: 521 WKDVFTPCWDATGLW--LWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSA 580
             +V+TP     G++  LW++AKA V  +D+G+HQL+SHW++TH S+EP++IA +RQLS 
Sbjct: 489 VSEVYTP---GEGVYDSLWQLAKAFVGVNDSGNHQLISHWMQTHASIEPFVIATNRQLSV 548

Query: 581 MHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLE 640
           +HP+++LL PHFR TM  NA+ARQ LIN GGI E T  P KY+ME SS  Y   W F  +
Sbjct: 549 LHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMSSFIYKNHWTFPDQ 608

Query: 641 ALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVV 700
           ALPA+L  RG+AVEDP APHGL+L I+DYPYA DGL +W AI+ W  +Y+  +Y     +
Sbjct: 609 ALPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWYAIESWVRDYIFLFYKIEEDI 668

Query: 701 KSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYS 760
           ++D ELQAWW E+R  GHGDKK EPWW  + T E+L++  T I+WV S  HA VNFGQY 
Sbjct: 669 QTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIIIWVASALHAAVNFGQYP 728

Query: 761 FAKYFPNRPSIARVNMPTEEPMKLWKYFLEK-PENVFLDTFPTQTQATIVSSVMNILSSH 820
            A Y PNRP+I+R  MP E   +  +  LEK P+ VFL T   Q Q  +  S++ ILS+H
Sbjct: 729 VAGYLPNRPTISRQYMPKENTPEFEE--LEKNPDKVFLKTITAQLQTLLGISLIEILSTH 788

Query: 821 SSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGVEPYQ 830
           SSDE YLG+     WA E     AFEKF  K+K++E+ IDERN +  LKNR G    PY 
Sbjct: 789 SSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYT 848