Moc02g00630 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g00630
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionBifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Locationchr2: 513185 .. 514345 (-)
RNA-Seq ExpressionMoc02g00630
SyntenyMoc02g00630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTCAAAATGGTGCGCGTTGGCGTTGGCGGTGGTGGTGCTTCTGGTGGGTTGTGTGAGGTCCAATATAGAGCAGGACAGGGCGGAGTGCGCGGACCAGGTGATTGGGCTGGCCACATGCCTGCCGTACGTGGGCGGGGCGGCCAAAGCGCCGACGCCGGACTGCTGCGGCGGCCTCAAGCTCGTGCTTCAAAAGAGTAAGAAATGTCTTTGCGTGTTGATAAAGGACCGTGATGACCCCACCCTCGGCCTCAAAGTTAACCTCACTCTTGCTCTTGGACTCCCCACTGCCTGCCATGCCCCTGCCGACATAGCCGAATGCGTCGGTAATTAACTCGTAACCATTTGGTTTCTAGTTTTCATAACAAAATTCAAATAATAATAATTTTGTTGTTACCACCTTCTGTATGTGAATTAGACCTTCTGCATCTGCCGCCAAACTCCCAAGAAGCCAAGGACTTTCTGGGGTCTAAAAATAACACCACTGCTGCAACCACTCCTTCAGCAGCTGCAGGTAATGAAAATTCCTTCGTCTCTTTAATCTTTCTTTTCTCTTTTCTAAATCCTTAATTTGGTTCATCAATTTTTTAAATCGTTTTATTTTGGTATTTAAACTTCTAAATTTTCTATCTTTTCCCTGGACTTAATTACAACAACAACAAAAAAAACCCATTTTGGATCAACGTTGATATTTTTCAATTTAGCAAAGTGGATACCTTGATTTTTTAGCTTGTACATTAATGTGTTGACCTAGGTGAGGCATGTATTTGAGCTAAACAAGTGGACTAAGGAGAGTTTTGAGTAAGTAGTTGCATTTACTAAAATAACGTTTTAAATTTAAAATTTATAGACATTTTGAAAATTCAACTAAAATAAGATTGAAATTTACTTTTTGGGTTTTTAGGAAAAAGAAGACTTCCCTCCTAAATAAATGAGTGAATCTTACAAATATTTTTTATCTACTTAAAAATTAAAAAAAAAAAAACCCCTTTTTATTATGGTGTGGAATTGTTGTGTGTTTGTTGCAGCGAGTGGGAATTCTACAAAAAGTGGAGAAAATCCACAAGGGAAAAGTGATGGTGGAATGAGGAAACAATGGTTCGGCGTGGAGATATTCCTTTGGTTTTTCACTTCATTGCTTCTACTAATTTGTATTTGA

mRNA sequence

ATGGGCTCAAAATGGTGCGCGTTGGCGTTGGCGGTGGTGGTGCTTCTGGTGGGTTGTGTGAGGTCCAATATAGAGCAGGACAGGGCGGAGTGCGCGGACCAGGTGATTGGGCTGGCCACATGCCTGCCGTACGTGGGCGGGGCGGCCAAAGCGCCGACGCCGGACTGCTGCGGCGGCCTCAAGCTCGTGCTTCAAAAGAGTAAGAAATGTCTTTGCGTGTTGATAAAGGACCGTGATGACCCCACCCTCGGCCTCAAAGTTAACCTCACTCTTGCTCTTGGACTCCCCACTGCCTGCCATGCCCCTGCCGACATAGCCGAATGCGTCGACCTTCTGCATCTGCCGCCAAACTCCCAAGAAGCCAAGGACTTTCTGGGGTCTAAAAATAACACCACTGCTGCAACCACTCCTTCAGCAGCTGCAGCGAGTGGGAATTCTACAAAAAGTGGAGAAAATCCACAAGGGAAAAGTGATGGTGGAATGAGGAAACAATGGTTCGGCGTGGAGATATTCCTTTGGTTTTTCACTTCATTGCTTCTACTAATTTGTATTTGA

Coding sequence (CDS)

ATGGGCTCAAAATGGTGCGCGTTGGCGTTGGCGGTGGTGGTGCTTCTGGTGGGTTGTGTGAGGTCCAATATAGAGCAGGACAGGGCGGAGTGCGCGGACCAGGTGATTGGGCTGGCCACATGCCTGCCGTACGTGGGCGGGGCGGCCAAAGCGCCGACGCCGGACTGCTGCGGCGGCCTCAAGCTCGTGCTTCAAAAGAGTAAGAAATGTCTTTGCGTGTTGATAAAGGACCGTGATGACCCCACCCTCGGCCTCAAAGTTAACCTCACTCTTGCTCTTGGACTCCCCACTGCCTGCCATGCCCCTGCCGACATAGCCGAATGCGTCGACCTTCTGCATCTGCCGCCAAACTCCCAAGAAGCCAAGGACTTTCTGGGGTCTAAAAATAACACCACTGCTGCAACCACTCCTTCAGCAGCTGCAGCGAGTGGGAATTCTACAAAAAGTGGAGAAAATCCACAAGGGAAAAGTGATGGTGGAATGAGGAAACAATGGTTCGGCGTGGAGATATTCCTTTGGTTTTTCACTTCATTGCTTCTACTAATTTGTATTTGA

Protein sequence

MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI
Homology
BLAST of Moc02g00630 vs. NCBI nr
Match: XP_022153758.1 (protein YLS3-like [Momordica charantia])

HSP 1 Score: 369.4 bits (947), Expect = 1.8e-98
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0

Query: 1   MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL 60
           MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL
Sbjct: 1   MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL 60

Query: 61  KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE 120
           KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE
Sbjct: 61  KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE 120

Query: 121 AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLL 180
           AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLL
Sbjct: 121 AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLL 180

Query: 181 LICI 185
           LICI
Sbjct: 181 LICI 184

BLAST of Moc02g00630 vs. NCBI nr
Match: XP_038900291.1 (non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida])

HSP 1 Score: 244.2 bits (622), Expect = 8.8e-61
Identity = 125/185 (67.57%), Postives = 143/185 (77.30%), Query Frame = 0

Query: 1   MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL 60
           M S    L + + +L VG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC GL
Sbjct: 1   MASTSTHLLIVMALLFVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGL 60

Query: 61  KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE 120
            LVLQKS+KCLC+LIKDRDDP+LGLKVNLTLALGLPTACH PADI +C+ LLHL PNS+E
Sbjct: 61  NLVLQKSRKCLCILIKDRDDPSLGLKVNLTLALGLPTACHTPADIKDCIGLLHLSPNSKE 120

Query: 121 AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGG-MRKQWFGVEIFLWFFTSLL 180
           AKDFL +    +  T+  A A SGNST   +N + K+DGG  R QW GVEIF+WF TS  
Sbjct: 121 AKDFLETNPKGSNTTSAPATAVSGNST---QNSEAKNDGGTKRNQWLGVEIFVWFSTSSF 180

Query: 181 LLICI 185
           LL CI
Sbjct: 181 LLTCI 182

BLAST of Moc02g00630 vs. NCBI nr
Match: XP_011653558.1 (protein YLS3 [Cucumis sativus] >KGN54079.1 hypothetical protein Csa_018100 [Cucumis sativus])

HSP 1 Score: 237.3 bits (604), Expect = 1.1e-58
Identity = 122/179 (68.16%), Postives = 141/179 (78.77%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           L  A+ +L VG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC GLKLVL KS
Sbjct: 9   LFAALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKS 68

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
           +KCLCVLIKDRDDP+LGLKVNL+LALGLP+ACHAPA+I +CV LLHL PNS EAKDFLGS
Sbjct: 69  RKCLCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGS 128

Query: 128 KNN--TTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
            N+  T  +T   A   SG+S+   +N + K+DG  R QW GVE+F+WF TS  L  CI
Sbjct: 129 PNSKETNTSTPAHATPVSGSSS---QNSEVKNDGTKRNQWLGVEMFIWFITSSFLFTCI 184

BLAST of Moc02g00630 vs. NCBI nr
Match: XP_023512874.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235.0 bits (598), Expect = 5.3e-58
Identity = 123/178 (69.10%), Postives = 144/178 (80.90%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           ++ A+ +LLVG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAMALLLVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+G+
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGT 133

Query: 128 KN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
           K  NTTAA    A AA GNST   + P  K   GMR QW GVE+F+ F TS LL  CI
Sbjct: 134 KGANTTAA---PATAAPGNST---QTPVEKG-SGMRNQWLGVEMFIGFLTSSLLFTCI 184

BLAST of Moc02g00630 vs. NCBI nr
Match: XP_022986686.1 (protein YLS3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 1.2e-57
Identity = 121/178 (67.98%), Postives = 142/178 (79.78%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           ++ A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GS
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGS 133

Query: 128 KN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
           K  NTTAA    A AA GNST   + P  K   GMR QW G E+F+ F TS LL  C+
Sbjct: 134 KGANTTAA---PATAAPGNST---QTPMEKG-SGMRNQWLGAEMFIAFLTSSLLFTCV 184

BLAST of Moc02g00630 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 2.4e-37
Identity = 79/175 (45.14%), Postives = 116/175 (66.29%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           + + +  +L+G   S++ QDR EC +Q+I L+TC+PYVGG AKAPT DCC G   V++KS
Sbjct: 10  ITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIRKS 69

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPA-DIAECVDLLHLPPNSQEAKDF-- 127
           +KC+C+L++D+DDP LG+K+N TLA  LP+ACH  A +I +C+ +LHLP NS  AK+F  
Sbjct: 70  EKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEFEN 129

Query: 128 LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLL 180
           LG   +   +T+P+     G  T  G+    KS+G   K W GVE+ ++   SL+
Sbjct: 130 LGRIEDNYNSTSPTQIHKDG--TGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLI 182

BLAST of Moc02g00630 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 79/194 (40.72%), Postives = 116/194 (59.79%), Query Frame = 0

Query: 7   ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQK 66
           A+AL +V ++V    ++  +D+ EC  Q++G+ATCLPYV G AK+PTPDCC GLK V+  
Sbjct: 12  AIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINS 71

Query: 67  SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF-- 126
             KCLC++I++R+DP LGL+VN++LAL LP+ CHA ADI +C  LLHL PNS +A+ F  
Sbjct: 72  DMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQ 131

Query: 127 --------------LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEI 185
                          GS +  T+ ++   ++A  NS ++   P         KQW G+E+
Sbjct: 132 LAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLEV 191

BLAST of Moc02g00630 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 82/194 (42.27%), Postives = 120/194 (61.86%), Query Frame = 0

Query: 7   ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQK 66
           A+AL VV ++V     +  +D+ EC +Q++G+ATCLPYV G AK+PTPDCC GLK VL  
Sbjct: 12  AIALIVVAMVVAAA-DDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNS 71

Query: 67  SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF-- 126
           +KKCLCV+I+DR+DP LGL++N++LAL LP+ CHA AD+ +C  LLHL PNS +A+ F  
Sbjct: 72  NKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQ 131

Query: 127 ----LGSKNNTTAAT---------TPSAAAASGNSTKSGENPQGKSDG-GMRKQWFGVEI 185
               L      +A T         +P++ +  GN++    +  G++      KQW G+E+
Sbjct: 132 LAKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLEV 191

BLAST of Moc02g00630 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.6e-25
Identity = 68/163 (41.72%), Postives = 95/163 (58.28%), Query Frame = 0

Query: 7   ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQK 66
           AL + V V+ +G VRS + QD   C D +  L +CLP+V   AKAP   CC  LK+ + K
Sbjct: 15  ALCITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDK 74

Query: 67  --SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF 126
             ++KCLC L+KDRDDP LG KV+   A+ LP+ACH PA+I++C DLLHL P+S  A   
Sbjct: 75  GQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDS-PASQI 134

Query: 127 LGSKNNTTAATTPSAAAASGNSTKSGENPQ-GKSDGGMRKQWF 167
                 +++ T    A ++ +S K  +N Q G    G    W+
Sbjct: 135 FKQFTESSSQTVGHKAVSTSSSIKGRDNKQFGLMMAGALSIWY 175

BLAST of Moc02g00630 vs. ExPASy Swiss-Prot
Match: F4HZB9 (Non-specific lipid transfer protein GPI-anchored 19 OS=Arabidopsis thaliana OX=3702 GN=LTPG19 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.1e-09
Identity = 51/177 (28.81%), Postives = 77/177 (43.50%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           LAL +   L G         +A C D +  L+ CL Y+ G + +P+  CC     V+Q S
Sbjct: 6   LALVIATFLYG----GATTVQAGCRDTLTSLSPCLYYLNGGSSSPSWSCCRQFSTVVQSS 65

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
            +CLC ++   +    G K N TLAL LPTAC+         +     P +  A   +GS
Sbjct: 66  PECLCSVVNSNESSFYGFKFNRTLALNLPTACNVQTPSPSLCNTGGNVPTTLPANTPVGS 125

Query: 128 KNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
                +A +PS   +  N T SG      S+    K      + +  F S+ L++ I
Sbjct: 126 PR---SAPSPSGTTSPAN-TPSGSKKFPLSNESSSKS----NVIILSFVSIALVLAI 170

BLAST of Moc02g00630 vs. ExPASy TrEMBL
Match: A0A6J1DIB7 (protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111021200 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 8.8e-99
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0

Query: 1   MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL 60
           MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL
Sbjct: 1   MGSKWCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGL 60

Query: 61  KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE 120
           KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE
Sbjct: 61  KLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQE 120

Query: 121 AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLL 180
           AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLL
Sbjct: 121 AKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLL 180

Query: 181 LICI 185
           LICI
Sbjct: 181 LICI 184

BLAST of Moc02g00630 vs. ExPASy TrEMBL
Match: A0A0A0KZ48 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 5.2e-59
Identity = 122/179 (68.16%), Postives = 141/179 (78.77%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           L  A+ +L VG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC GLKLVL KS
Sbjct: 9   LFAALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKS 68

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
           +KCLCVLIKDRDDP+LGLKVNL+LALGLP+ACHAPA+I +CV LLHL PNS EAKDFLGS
Sbjct: 69  RKCLCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGS 128

Query: 128 KNN--TTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
            N+  T  +T   A   SG+S+   +N + K+DG  R QW GVE+F+WF TS  L  CI
Sbjct: 129 PNSKETNTSTPAHATPVSGSSS---QNSEVKNDGTKRNQWLGVEMFIWFITSSFLFTCI 184

BLAST of Moc02g00630 vs. ExPASy TrEMBL
Match: A0A6J1JBX4 (protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.8e-58
Identity = 121/178 (67.98%), Postives = 142/178 (79.78%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           ++ A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GS
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGS 133

Query: 128 KN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
           K  NTTAA    A AA GNST   + P  K   GMR QW G E+F+ F TS LL  C+
Sbjct: 134 KGANTTAA---PATAAPGNST---QTPMEKG-SGMRNQWLGAEMFIAFLTSSLLFTCV 184

BLAST of Moc02g00630 vs. ExPASy TrEMBL
Match: A0A6J1J894 (protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-57
Identity = 118/177 (66.67%), Postives = 140/177 (79.10%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           ++ A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 127
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GS
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGS 133

Query: 128 KNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
           K     A T +A AA GNST   + P  K   GMR QW G E+F+ F TS LL  C+
Sbjct: 134 K----GANTTAAPAAPGNST---QTPMEKG-SGMRNQWLGAEMFIAFLTSSLLFTCV 182

BLAST of Moc02g00630 vs. ExPASy TrEMBL
Match: A0A6J1FVR4 (protein YLS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-57
Identity = 126/180 (70.00%), Postives = 143/180 (79.44%), Query Frame = 0

Query: 6   CALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQ 65
           CA+AL    L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+
Sbjct: 16  CAMAL----LFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLE 75

Query: 66  KSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFL 125
           KSKKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+
Sbjct: 76  KSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFM 135

Query: 126 GSKN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 185
           GSK  NTTAA    A AA GNST+S   P  K   GMR QW  VE+F+ F TS LL  CI
Sbjct: 136 GSKGANTTAA---PAFAAPGNSTQS---PMEKG-SGMRNQWSSVEMFIGFLTSSLLFTCI 184

BLAST of Moc02g00630 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-38
Identity = 79/175 (45.14%), Postives = 116/175 (66.29%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           + + +  +L+G   S++ QDR EC +Q+I L+TC+PYVGG AKAPT DCC G   V++KS
Sbjct: 53  ITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIRKS 112

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPA-DIAECVDLLHLPPNSQEAKDF-- 127
           +KC+C+L++D+DDP LG+K+N TLA  LP+ACH  A +I +C+ +LHLP NS  AK+F  
Sbjct: 113 EKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEFEN 172

Query: 128 LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLL 180
           LG   +   +T+P+     G  T  G+    KS+G   K W GVE+ ++   SL+
Sbjct: 173 LGRIEDNYNSTSPTQIHKDG--TGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLI 225

BLAST of Moc02g00630 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 5.0e-38
Identity = 78/175 (44.57%), Postives = 115/175 (65.71%), Query Frame = 0

Query: 8   LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 67
           + + +  +L+G   S++ QDR EC +Q+I L+TC+PYVGG AKAPT DCC G   V++KS
Sbjct: 53  ITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIRKS 112

Query: 68  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPA-DIAECVDLLHLPPNSQEAKDF-- 127
           +KC+C+L++D+DDP LG+K+N TLA  LP+ACH  A +I +C+ +LHLP NS  AK+F  
Sbjct: 113 EKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEFEN 172

Query: 128 LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLL 180
           LG   +   +T+P+     G     G+    KS+G   K W GVE+ ++   SL+
Sbjct: 173 LGRIEDNYNSTSPTQIHKGG-----GKAEPVKSNGWKEKSWLGVELLIYLLVSLI 222

BLAST of Moc02g00630 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 9.4e-37
Identity = 79/194 (40.72%), Postives = 116/194 (59.79%), Query Frame = 0

Query: 7   ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQK 66
           A+AL +V ++V    ++  +D+ EC  Q++G+ATCLPYV G AK+PTPDCC GLK V+  
Sbjct: 12  AIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINS 71

Query: 67  SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF-- 126
             KCLC++I++R+DP LGL+VN++LAL LP+ CHA ADI +C  LLHL PNS +A+ F  
Sbjct: 72  DMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQ 131

Query: 127 --------------LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEI 185
                          GS +  T+ ++   ++A  NS ++   P         KQW G+E+
Sbjct: 132 LAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLEV 191

BLAST of Moc02g00630 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-36
Identity = 82/194 (42.27%), Postives = 120/194 (61.86%), Query Frame = 0

Query: 7   ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQK 66
           A+AL VV ++V     +  +D+ EC +Q++G+ATCLPYV G AK+PTPDCC GLK VL  
Sbjct: 12  AIALIVVAMVVAAA-DDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNS 71

Query: 67  SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF-- 126
           +KKCLCV+I+DR+DP LGL++N++LAL LP+ CHA AD+ +C  LLHL PNS +A+ F  
Sbjct: 72  NKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQ 131

Query: 127 ----LGSKNNTTAAT---------TPSAAAASGNSTKSGENPQGKSDG-GMRKQWFGVEI 185
               L      +A T         +P++ +  GN++    +  G++      KQW G+E+
Sbjct: 132 LAKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLEV 191

BLAST of Moc02g00630 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-26
Identity = 68/163 (41.72%), Postives = 95/163 (58.28%), Query Frame = 0

Query: 7   ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQK 66
           AL + V V+ +G VRS + QD   C D +  L +CLP+V   AKAP   CC  LK+ + K
Sbjct: 15  ALCITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDK 74

Query: 67  --SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF 126
             ++KCLC L+KDRDDP LG KV+   A+ LP+ACH PA+I++C DLLHL P+S  A   
Sbjct: 75  GQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDS-PASQI 134

Query: 127 LGSKNNTTAATTPSAAAASGNSTKSGENPQ-GKSDGGMRKQWF 167
                 +++ T    A ++ +S K  +N Q G    G    W+
Sbjct: 135 FKQFTESSSQTVGHKAVSTSSSIKGRDNKQFGLMMAGALSIWY 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153758.11.8e-98100.00protein YLS3-like [Momordica charantia][more]
XP_038900291.18.8e-6167.57non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida][more]
XP_011653558.11.1e-5868.16protein YLS3 [Cucumis sativus] >KGN54079.1 hypothetical protein Csa_018100 [Cucu... [more]
XP_023512874.15.3e-5869.10protein YLS3-like [Cucurbita pepo subsp. pepo][more]
XP_022986686.11.2e-5767.98protein YLS3-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4I0822.4e-3745.14Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37... [more]
O648641.3e-3540.72Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3... [more]
O648651.7e-3542.27Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3... [more]
Q9M2G11.6e-2541.72Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3... [more]
F4HZB92.1e-0928.81Non-specific lipid transfer protein GPI-anchored 19 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1DIB78.8e-99100.00protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111021200 PE=4 SV=1[more]
A0A0A0KZ485.2e-5968.16AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV... [more]
A0A6J1JBX45.8e-5867.98protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV... [more]
A0A6J1J8941.3e-5766.67protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV... [more]
A0A6J1FVR41.3e-5770.00protein YLS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G55260.11.7e-3845.14Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G55260.25.0e-3844.57Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT2G44290.19.4e-3740.72Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT2G44300.11.2e-3642.27Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G58550.11.2e-2641.72Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 31..108
e-value: 0.0075
score: 25.5
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF14368LTP_2coord: 14..108
e-value: 2.2E-12
score: 46.9
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 28..106
e-value: 3.5E-13
score: 51.2
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 30..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..158
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..154
NoneNo IPR availablePANTHERPTHR33044:SF194BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEINcoord: 7..174
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..19
score: 5.0
NoneNo IPR availableCDDcd00010AAI_LTSScoord: 38..100
e-value: 7.08728E-17
score: 69.3778
IPR043325Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) ProteinPANTHERPTHR33044BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATEDcoord: 7..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g00630.1Moc02g00630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane