Moc02g00570 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g00570
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr2: 464013 .. 465163 (-)
RNA-Seq Expression	Moc02g00570
Synteny	Moc02g00570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACGGCAATGGCAATGGCTCCAAGAAGCGCTTCAGGAACAGGAAACGAAGAAGAAGAAGTTGATGAGACTGATTTTGGCATAACCAAAGGGAATGGAGTGAAAGTGCTTTCCCAAATGGGCCTCACAGCTCTCCCTCTCCAATTCATTCAGCCTCCGGAGGAGAGATTCAGAACCATGTCGTCCGTCTCCATGCTACCCATAATCGACATGTCACACGACGCCGCCGATTCCATCTGCGACGCCGCCGAGAACTGGGGCGTCTTCCAGGTGACGAACCACGGGGTTCCTCCGCGAGTGCTCCACAATGCCATGGCTGCGGCTCGCCGCTTCTTCGACATGTCGCCGGAGGAGAAGAGCCGCTTCTTCAAGTTGAACGACAACTCGGCAACCTACGGCACCAACTTCGGCACTTCGCCCGAGAAAGCCTTGGAGTAGAAAGATTATTTGAACCTCTTTTACGTTTCTGATGATCACGCTTCTGCCCTCTGGCCGCCCCTATGCAGGTACCAATTCAACCTAGATTCGATTCTGTTGGTGTAACCTAATTTGTTGTATATGCATACAGAGATGAAATGGTGGAGTACGTGAAGAGCTCGGAAGGAGTTGTAAAACGAGTGGTTGAGATTCTGATGGAGAAGTTAGGCGTCGTAGAAATTGACGAAACCAAACGATCTCTGTTAATGGGTTCCAAGAGAATCAAGCTTAACTACTACCCCATTTGTCCAAACCCTCACCTCGCCCTCGGGGTCGGCCGCCACTCCGACGTTTCCACGCTGACCGTCCTTCTGCAGGACCACGTCGGCGGCCTCCACGTGCGGCGACCAACCCACGGCGACGCCACATGGGTTCACGCCTCGCCGGTGCCAGGATCGCTGGTGATCAACATTGGGGATGCGCTGCAAATAATGAGCAACGGGCGATTCAAGAGCGTGGAGCATTTCGTGGCGGCCAATGGAAGCAGCAACAGAATCTCGATTCCCATCTTTGTGAACCCAGAGCCGTCCGCCATCATTGGCCCTCTGTCGGAGGTTCTTGCCACCGGACAGAAACCAGAGTACAAGCAAGTTCTGTATTCGGATTACGTCAGGCATTTCTACAACAACCCTCATCATGGCAAGAACAGCATTGAGTTTGCCAAGATCTGA

mRNA sequence

ATGGCAACGGCAATGGCAATGGCTCCAAGAAGCGCTTCAGGAACAGGAAACGAAGAAGAAGAAGTTGATGAGACTGATTTTGGCATAACCAAAGGGAATGGAGTGAAAGTGCTTTCCCAAATGGGCCTCACAGCTCTCCCTCTCCAATTCATTCAGCCTCCGGAGGAGAGATTCAGAACCATGTCGTCCGTCTCCATGCTACCCATAATCGACATGTCACACGACGCCGCCGATTCCATCTGCGACGCCGCCGAGAACTGGGGCGTCTTCCAGGTGACGAACCACGGGGTTCCTCCGCGAGTGCTCCACAATGCCATGGCTGCGGCTCGCCGCTTCTTCGACATGTCGCCGGAGGAGAAGAGCCGCTTCTTCAAGTTGAACGACAACTCGGCAACCTACGGCACCAACTTCGGCACTTCGCCCGAGAAAGCCTTGGAAGATGAAATGGTGGAGTACGTGAAGAGCTCGGAAGGAGTTGTAAAACGAGTGGTTGAGATTCTGATGGAGAAGTTAGGCGTCGTAGAAATTGACGAAACCAAACGATCTCTGTTAATGGGTTCCAAGAGAATCAAGCTTAACTACTACCCCATTTGTCCAAACCCTCACCTCGCCCTCGGGGTCGGCCGCCACTCCGACGTTTCCACGCTGACCGTCCTTCTGCAGGACCACGTCGGCGGCCTCCACGTGCGGCGACCAACCCACGGCGACGCCACATGGGTTCACGCCTCGCCGGTGCCAGGATCGCTGGTGATCAACATTGGGGATGCGCTGCAAATAATGAGCAACGGGCGATTCAAGAGCGTGGAGCATTTCGTGGCGGCCAATGGAAGCAGCAACAGAATCTCGATTCCCATCTTTGTGAACCCAGAGCCGTCCGCCATCATTGGCCCTCTGTCGGAGGTTCTTGCCACCGGACAGAAACCAGAGTACAAGCAAGTTCTGTATTCGGATTACGTCAGGCATTTCTACAACAACCCTCATCATGGCAAGAACAGCATTGAGTTTGCCAAGATCTGA

Coding sequence (CDS)

ATGGCAACGGCAATGGCAATGGCTCCAAGAAGCGCTTCAGGAACAGGAAACGAAGAAGAAGAAGTTGATGAGACTGATTTTGGCATAACCAAAGGGAATGGAGTGAAAGTGCTTTCCCAAATGGGCCTCACAGCTCTCCCTCTCCAATTCATTCAGCCTCCGGAGGAGAGATTCAGAACCATGTCGTCCGTCTCCATGCTACCCATAATCGACATGTCACACGACGCCGCCGATTCCATCTGCGACGCCGCCGAGAACTGGGGCGTCTTCCAGGTGACGAACCACGGGGTTCCTCCGCGAGTGCTCCACAATGCCATGGCTGCGGCTCGCCGCTTCTTCGACATGTCGCCGGAGGAGAAGAGCCGCTTCTTCAAGTTGAACGACAACTCGGCAACCTACGGCACCAACTTCGGCACTTCGCCCGAGAAAGCCTTGGAAGATGAAATGGTGGAGTACGTGAAGAGCTCGGAAGGAGTTGTAAAACGAGTGGTTGAGATTCTGATGGAGAAGTTAGGCGTCGTAGAAATTGACGAAACCAAACGATCTCTGTTAATGGGTTCCAAGAGAATCAAGCTTAACTACTACCCCATTTGTCCAAACCCTCACCTCGCCCTCGGGGTCGGCCGCCACTCCGACGTTTCCACGCTGACCGTCCTTCTGCAGGACCACGTCGGCGGCCTCCACGTGCGGCGACCAACCCACGGCGACGCCACATGGGTTCACGCCTCGCCGGTGCCAGGATCGCTGGTGATCAACATTGGGGATGCGCTGCAAATAATGAGCAACGGGCGATTCAAGAGCGTGGAGCATTTCGTGGCGGCCAATGGAAGCAGCAACAGAATCTCGATTCCCATCTTTGTGAACCCAGAGCCGTCCGCCATCATTGGCCCTCTGTCGGAGGTTCTTGCCACCGGACAGAAACCAGAGTACAAGCAAGTTCTGTATTCGGATTACGTCAGGCATTTCTACAACAACCCTCATCATGGCAAGAACAGCATTGAGTTTGCCAAGATCTGA

Protein sequence

MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLNDNSATYGTNFGTSPEKALEDEMVEYVKSSEGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI

Homology

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BLAST of Moc02g00570 vs. NCBI nr
Match: XP_022153808.1 (LOW QUALITY PROTEIN: feruloyl CoA ortho-hydroxylase 1 [Momordica charantia])

HSP 1 Score: 666.0 bits (1717), Expect = 1.7e-187
Identity = 338/362 (93.37%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRT 60
           MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRT
Sbjct: 1   MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRT 60

Query: 61  MSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK 120
           MSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK
Sbjct: 61  MSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK 120

Query: 121 SRFFKLNDNSATYGTNFGTSPEKALE------------------------DEMVEYVKSS 180
           SRFFKLNDNSATYGTNFGTSPEKALE                        DEMVEYVKSS
Sbjct: 121 SRFFKLNDNSATYGTNFGTSPEKALEXKDYLNLFYVSDDHASALWPPLCRDEMVEYVKSS 180

Query: 181 EGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTL 240
           EGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTL
Sbjct: 181 EGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTL 240

Query: 241 TVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANG 300
           TVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANG
Sbjct: 241 TVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANG 300

Query: 301 SSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFA 339
           SSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFA
Sbjct: 301 SSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFA 360

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BLAST of Moc02g00570 vs. NCBI nr
Match: KAE9462342.1 (hypothetical protein C3L33_05761, partial [Rhododendron williamsianum])

HSP 1 Score: 381.3 bits (978), Expect = 8.5e-102
Identity = 198/364 (54.40%), Postives = 252/364 (69.23%), Query Frame = 0


Query: 7   MAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV-- 66
           MAP  +  + ++ + +D T F + +GNGVK LS++GL  LP Q+IQP EER  +MS +  
Sbjct: 1   MAPSLSLISDSDSDSLDVTKFAVYQGNGVKGLSELGLKTLPKQYIQPVEERMSSMSHIEX 60

Query: 67  SMLPIIDMSH----DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK 126
             +PIIDM++      AD+ICDAAE WG FQ+ NHGVP  VL +   A  RFF +  EEK
Sbjct: 61  ESIPIIDMANWDDPKVADAICDAAEKWGFFQIINHGVPIEVLEDVKTATHRFFGLPAEEK 120

Query: 127 SRFFKLND--NSATYGTNFGTSPEKALE------------------------DEMVEYVK 186
            ++ K N   N+  YGT+F    EKALE                        D+ +E+++
Sbjct: 121 GKYSKENSCTNNVRYGTSFSPQAEKALEWKDYLSLFYVSDGEAASRWPSQCKDQALEFMR 180

Query: 187 SSEGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVS 246
            S+  +KR++E L ++L V E+DET ++LLMGSKRI LNYYPICPNP L +GVGRHSDVS
Sbjct: 181 RSDCAIKRLLEALTKRLNVKEMDETHQTLLMGSKRINLNYYPICPNPELTVGVGRHSDVS 240

Query: 247 TLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAA 306
           TLT+LLQD +GGL+VR+    D TW+H  P+ GSLVINIGDALQIMSNGR+KSVEH V A
Sbjct: 241 TLTILLQDEIGGLYVRKLDDED-TWIHVPPISGSLVINIGDALQIMSNGRYKSVEHRVMA 300

Query: 307 NGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIE 339
           NGS NRIS+PIFVNP PSAIIGPL EVL  G+KP YKQVLYSDYV++F+   H GK+++E
Sbjct: 301 NGSKNRISVPIFVNPXPSAIIGPLPEVLEGGEKPVYKQVLYSDYVKYFFRKGHDGKDTVE 360

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BLAST of Moc02g00570 vs. NCBI nr
Match: KAF3948390.1 (hypothetical protein CMV_025603 [Castanea mollissima])

HSP 1 Score: 380.9 bits (977), Expect = 1.1e-101
Identity = 206/365 (56.44%), Postives = 253/365 (69.32%), Query Frame = 0


Query: 7   MAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV-- 66
           MAP  A+      E  + TDF +T GNGVK LS+MGL  LP Q+IQP EER  T+S++  
Sbjct: 1   MAPTVATPI---SESSNITDFVVTNGNGVKGLSEMGLKTLPKQYIQPVEERI-TVSNILP 60

Query: 67  -SMLPIIDMSH----DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEE 126
              +PIIDMS+      ++SICDAAE WG FQ+ NHGVP  VL N   A  RFF++  EE
Sbjct: 61  QESIPIIDMSNWDEPKVSESICDAAEKWGFFQIINHGVPIEVLENVKDATHRFFNLPAEE 120

Query: 127 KSRFFKLN--DNSATYGTNFGTSPEKALE------------------------DEMVEYV 186
           K +F K N   NS  +GT+F    EKALE                        D+++EY+
Sbjct: 121 KRKFSKENSPSNSVRFGTSFSPEAEKALEWKDYLSLFYVSEDEASALWPSACKDQVLEYM 180

Query: 187 KSSEGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDV 246
           + SE V++R++E LM+++ V EIDETK SLLMGSKRI LNYYPICPNP L +GVGRHSDV
Sbjct: 181 RRSEPVIQRLLEALMKRIHVKEIDETKESLLMGSKRINLNYYPICPNPELTVGVGRHSDV 240

Query: 247 STLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVA 306
           STLT+LLQD++GGL+VR     + +WVH  PV GSLVIN+GDALQIMSNGR+KS+EH V 
Sbjct: 241 STLTILLQDNIGGLYVR---GNNDSWVHVPPVSGSLVINVGDALQIMSNGRYKSIEHRVV 300

Query: 307 ANGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSI 339
           A+GS NRIS+PIFVNP P  +IGP SEVLA G+K  YKQVLYSDYV+HF+   H GKN+I
Sbjct: 301 ASGSKNRISVPIFVNPRPCDMIGPFSEVLAGGEKALYKQVLYSDYVKHFFRKAHDGKNTI 358

[Close]

BLAST of Moc02g00570 vs. NCBI nr
Match: XP_030952583.1 (feruloyl CoA ortho-hydroxylase F6H1-3-like [Quercus lobata])

HSP 1 Score: 379.8 bits (974), Expect = 2.5e-101
Identity = 200/352 (56.82%), Postives = 246/352 (69.89%), Query Frame = 0


Query: 20  EEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV---SMLPIIDMSH-- 79
           E  + TDF +T  NGVK LS+MGL  LP Q+IQP EER  T+S++     +PIIDMS+  
Sbjct: 11  ESSNITDFVVTNRNGVKGLSEMGLKTLPKQYIQPVEERI-TVSNILPQESIPIIDMSNWD 70

Query: 80  --DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN--DNS 139
               ++SICDAAE WG FQ+ NHGVP  VL N   A  RFF++  EEKS+F K N   NS
Sbjct: 71  EPKVSESICDAAEKWGFFQIINHGVPIEVLENVKDATHRFFNLPAEEKSKFSKENSPSNS 130

Query: 140 ATYGTNFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEI 199
             +GT+F    EKALE                        D+++EY++ SE V++R++E 
Sbjct: 131 VRFGTSFSPEAEKALEWKDYLSMFYVSEDEASALWPSACKDQVLEYMRGSEPVIQRLLEA 190

Query: 200 LMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGG 259
           LM+++ V EIDETK+SLLMGSKRI LNYYPICPNP L +G+GRHSDVSTLT+LLQD +GG
Sbjct: 191 LMKRINVKEIDETKKSLLMGSKRINLNYYPICPNPELTVGIGRHSDVSTLTILLQDDIGG 250

Query: 260 LHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIF 319
           L+VR     + +WVH  P+ GSLVIN+GDALQIMSNGR+KS+EH V ANGS NRIS+PIF
Sbjct: 251 LYVR---GNNDSWVHVPPISGSLVINVGDALQIMSNGRYKSIEHRVVANGSKNRISVPIF 310

Query: 320 VNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           VNP P  +IGP  EVLA G+K  YKQVLYS+YVRHFY   H GK +IEFAKI
Sbjct: 311 VNPRPDDMIGPFPEVLARGEKALYKQVLYSNYVRHFYKKAHDGKKTIEFAKI 358

[Close]

BLAST of Moc02g00570 vs. NCBI nr
Match: XP_030952454.1 (feruloyl CoA ortho-hydroxylase F6H1-3-like isoform X2 [Quercus lobata])

HSP 1 Score: 379.4 bits (973), Expect = 3.2e-101
Identity = 199/351 (56.70%), Postives = 243/351 (69.23%), Query Frame = 0


Query: 20  EEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERF--RTMSSVSMLPIIDMSH--- 79
           E  D +DF +TKGNGVK LS+MG   LP Q+IQP EER     + ++  +PIIDMS+   
Sbjct: 11  ESSDISDFVVTKGNGVKGLSEMGPKTLPRQYIQPLEERINVNNIMTLESIPIIDMSNWDE 70

Query: 80  -DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN--DNSA 139
              ++SICDAAE WG FQ+ NHGVP  VL N   A  RFF++  EEK +F K N   NS 
Sbjct: 71  PKVSESICDAAEKWGFFQIINHGVPIEVLENVKDATHRFFNLPAEEKRKFSKENSPSNSV 130

Query: 140 TYGTNFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEIL 199
            +GT+F    EKALE                        D+++EY+  SE V++R++E L
Sbjct: 131 RFGTSFSPEAEKALEWKDYLSMFYVSEDEASALWPSACKDQVLEYMGGSEPVIQRLLEAL 190

Query: 200 MEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGL 259
           M+++ V EIDETK+SLLMGSKRI LNYYPICPNP L +G+GRHSDVSTLT+LLQD VGGL
Sbjct: 191 MKRINVKEIDETKKSLLMGSKRINLNYYPICPNPELTVGIGRHSDVSTLTILLQDDVGGL 250

Query: 260 HVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFV 319
           +VR     + +WVH  P+ GSLVIN+GDALQIMSNGR+KS+EH V ANGS NRIS+PIFV
Sbjct: 251 YVR---GNNDSWVHVPPISGSLVINVGDALQIMSNGRYKSIEHRVVANGSKNRISVPIFV 310

Query: 320 NPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           NP P  +IGP  EVLA G+K  YKQVLYS+YVRHFY   H GK +IEFAKI
Sbjct: 311 NPRPDDMIGPFPEVLAGGEKALYKQVLYSNYVRHFYKKAHDGKKTIEFAKI 358

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BLAST of Moc02g00570 vs. ExPASy Swiss-Prot
Match: G9M9M1 (Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.9e-96
Identity = 182/347 (52.45%), Postives = 234/347 (67.44%), Query Frame = 0


Query: 25  TDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV--SMLPIIDMSH-----DAA 84
           TDF + +GNGVK L+ MGL ALP Q++QP EER  T + +    +P+ID+S         
Sbjct: 13  TDFVVHEGNGVKGLADMGLEALPKQYVQPEEERITTSTVIVDDTIPVIDLSEWGSDPKVG 72

Query: 85  DSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN--DNSATYGT 144
           D IC+AAE WG FQ+ NHGVP  VL    AA  RFF +  EEK++  K N   N+  YGT
Sbjct: 73  DMICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHCKDNSPSNNVRYGT 132

Query: 145 NFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEILMEKL 204
           +F    EKALE                        DE + ++++ + V+KR+++ LM+ L
Sbjct: 133 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 192

Query: 205 GVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGLHVRR 264
            V EID TK SLLMGSKRI +NYYP CPNP L +GVGRHSDVSTLT+LLQD +GGL+VR+
Sbjct: 193 NVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVRK 252

Query: 265 PTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFVNPEP 324
                 TWVH  P+ G++VIN+GDALQI+SNGR+KS+EH V ANGS+NRIS+PIFVNP P
Sbjct: 253 --LDSDTWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNPRP 312

Query: 325 SAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           + IIGPL E+L +G+K  YK VLYSDYV+HF+   H GK +++FAKI
Sbjct: 313 NDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

[Close]

BLAST of Moc02g00570 vs. ExPASy Swiss-Prot
Match: G9M9M2 (Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 4.2e-96
Identity = 182/347 (52.45%), Postives = 234/347 (67.44%), Query Frame = 0


Query: 25  TDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV--SMLPIIDMSH-----DAA 84
           TDF + +GNGVK L+ MGL +LP Q+IQP EER  T + +    +P+ID+S         
Sbjct: 13  TDFVVHEGNGVKGLADMGLQSLPKQYIQPAEERITTSTVIVDDTIPVIDLSEWGSDPKVG 72

Query: 85  DSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN--DNSATYGT 144
           D IC+AAE WG FQ+ NHGVP  VL    AA  RFF +  EEK++  K N   N+  YGT
Sbjct: 73  DLICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSPSNNVRYGT 132

Query: 145 NFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEILMEKL 204
           +F    EKALE                        DE + ++++ + V+KR+++ LM+ L
Sbjct: 133 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 192

Query: 205 GVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGLHVRR 264
            V EID TK SLLMGSKRI +NYYP CPNP L +GVGRHSDVSTLT+LLQD +GGL+VR+
Sbjct: 193 NVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVRK 252

Query: 265 PTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFVNPEP 324
                 TWVH  P+ G++VIN+GDALQI+SNGR+KS+EH V ANGS+NRIS+PIFVNP P
Sbjct: 253 --LDSDTWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNPRP 312

Query: 325 SAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           + IIGPL E+L +G+K  YK VLYSDYV+HF+   H GK +++FAKI
Sbjct: 313 NDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

[Close]

BLAST of Moc02g00570 vs. ExPASy Swiss-Prot
Match: G9M9M0 (Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.6e-95
Identity = 179/347 (51.59%), Postives = 233/347 (67.15%), Query Frame = 0


Query: 25  TDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV--SMLPIIDMSH-----DAA 84
           TDF + +GNGVK L+ MGL ALP Q++QP EER  T + +    +P+ID+S         
Sbjct: 13  TDFVVHEGNGVKGLADMGLEALPKQYVQPEEERITTSTVIVDDTIPVIDLSEWGSDPKVG 72

Query: 85  DSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN--DNSATYGT 144
           D  C+AAE WG FQ+ NHGVP  VL    AA  RFF +  EEK++  K N   N+  YGT
Sbjct: 73  DMNCEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSPSNNVRYGT 132

Query: 145 NFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEILMEKL 204
           +F    EKALE                        DE + ++++ + V+KR+++ LM+ L
Sbjct: 133 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 192

Query: 205 GVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGLHVRR 264
            V EID TK SLLMGSKRI +NYYP CPNP L +GVGRHSDVSTLT+LLQD +GGL+VR+
Sbjct: 193 NVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVRK 252

Query: 265 PTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFVNPEP 324
               +  WVH  P+ G++VIN+GDALQI+SNGR+KS+EH V ANGS+NRIS+PIFVNP P
Sbjct: 253 LDSDE--WVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNPRP 312

Query: 325 SAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           + +IGPL E+L +G+K  YK VLYSDYV+HF+   H GK +++FAKI
Sbjct: 313 NDVIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

[Close]

BLAST of Moc02g00570 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.1e-95
Identity = 187/365 (51.23%), Postives = 242/365 (66.30%), Query Frame = 0


Query: 7   MAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTM---SS 66
           MAP   +   +   EV  TDF + KGNGVK LS+ G+ ALP Q+IQP EER        +
Sbjct: 1   MAPTLLTTQFSNPAEV--TDFVVYKGNGVKGLSETGIKALPEQYIQPLEERLINKFVNET 60

Query: 67  VSMLPIIDMSHD----AADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEE 126
              +P+IDMS+      A+++CDAAE WG FQV NHGVP  VL +  AA  +FF++  EE
Sbjct: 61  DEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEE 120

Query: 127 KSRFFKLNDNSAT--YGTNFGTSPEKALE------------------------DEMVEYV 186
           K +F K N  S T  +GT+F    E+ALE                        +E +EY+
Sbjct: 121 KRKFTKENSLSTTVRFGTSFSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNETLEYI 180

Query: 187 KSSEGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDV 246
             S+ +V+R++E L + L V E+DETK SL MGS R+ LNYYPICPNP L +GVGRHSDV
Sbjct: 181 NKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGRHSDV 240

Query: 247 STLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVA 306
           S+LT+LLQD +GGLHVR    G+  WVH  PV GS VINIGDA+QIMSNG +KSVEH V 
Sbjct: 241 SSLTILLQDQIGGLHVRSLASGN--WVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHRVL 300

Query: 307 ANGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSI 339
           ANG +NRIS+PIFVNP+P ++IGPL EV+A G++P Y+ VLYSDYV++F+   H GK ++
Sbjct: 301 ANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKKTV 360

[Close]

BLAST of Moc02g00570 vs. ExPASy Swiss-Prot
Match: W5QJZ5 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox4 OS=Ruta graveolens OX=37565 GN=DIOX4 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 8.0e-95
Identity = 185/364 (50.82%), Postives = 240/364 (65.93%), Query Frame = 0


Query: 7   MAPRSASGTGNEEEEVDE-TDFGITKGNGVKVLSQMGLTALPLQFIQPPEERF--RTMSS 66
           MAP   S      E  DE ++F   KG+GVK LS++G+  LP QF QP EERF  + +  
Sbjct: 1   MAPTKDSVIHMGAESWDEISEFVTKKGHGVKGLSELGIKTLPKQFHQPLEERFSEKKILE 60

Query: 67  VSMLPIIDMSH----DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEE 126
            + +P+IDMS     +   SICDAAE+WG FQ+ NHGVP   L     A  RFF +  EE
Sbjct: 61  RASIPLIDMSKWDSPEVVKSICDAAEHWGFFQIVNHGVPLETLQRVKEATHRFFALPAEE 120

Query: 127 KSRFFKLND--NSATYGTNFGTSPEKALE------------------------DEMVEYV 186
           K+++ K N   N+  +G++F    EKALE                        DEM+EY+
Sbjct: 121 KNKYSKENSPINNVRFGSSFVPHVEKALEWKDFLSMFYVSEEETNTYWPPICRDEMLEYM 180

Query: 187 KSSEGVVKRVVEILMEK-LGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSD 246
           +SSE ++KR++E+L+ K L V +IDE +  +L+GS+RI LNYYP CPNP L LGVGRHSD
Sbjct: 181 RSSEVLIKRLMEVLVVKGLKVKQIDEIREPMLVGSRRINLNYYPKCPNPELTLGVGRHSD 240

Query: 247 VSTLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFV 306
           +ST T+LLQD +GGLHVR+      TWVH +P+ GSL+INIGDALQIMSNGR+KS+EH V
Sbjct: 241 ISTFTILLQDEIGGLHVRKLDDTGNTWVHVTPISGSLIINIGDALQIMSNGRYKSIEHMV 300

Query: 307 AANGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNS 337
            ANG+ +RIS+P+FVNP+P AI+ P  EVLA G+KP YK VL SDY RHFY  PH GK +
Sbjct: 301 VANGTQDRISVPLFVNPKPQAILCPFPEVLANGEKPVYKPVLCSDYSRHFYTKPHDGKKT 360

[Close]

BLAST of Moc02g00570 vs. ExPASy TrEMBL
Match: A0A6J1DIJ1 (LOW QUALITY PROTEIN: feruloyl CoA ortho-hydroxylase 1 OS=Momordica charantia OX=3673 GN=LOC111021233 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 8.3e-188
Identity = 338/362 (93.37%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRT 60
           MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRT
Sbjct: 1   MATAMAMAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRT 60

Query: 61  MSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK 120
           MSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK
Sbjct: 61  MSSVSMLPIIDMSHDAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK 120

Query: 121 SRFFKLNDNSATYGTNFGTSPEKALE------------------------DEMVEYVKSS 180
           SRFFKLNDNSATYGTNFGTSPEKALE                        DEMVEYVKSS
Sbjct: 121 SRFFKLNDNSATYGTNFGTSPEKALEXKDYLNLFYVSDDHASALWPPLCRDEMVEYVKSS 180

Query: 181 EGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTL 240
           EGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTL
Sbjct: 181 EGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTL 240

Query: 241 TVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANG 300
           TVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANG
Sbjct: 241 TVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANG 300

Query: 301 SSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFA 339
           SSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFA
Sbjct: 301 SSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFA 360

[Close]

BLAST of Moc02g00570 vs. ExPASy TrEMBL
Match: A0A2N9GN50 (Fe2OG dioxygenase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS28825 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 8.3e-103
Identity = 203/352 (57.67%), Postives = 245/352 (69.60%), Query Frame = 0


Query: 20  EEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV---SMLPIIDMSH-- 79
           E  D TDF ++KGNGVK LS+MGL  LP Q+IQP EER  +MS++     +PIIDMS+  
Sbjct: 11  ESSDITDFVVSKGNGVKGLSEMGLKTLPNQYIQPLEERL-SMSNIMPLESIPIIDMSNWD 70

Query: 80  --DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLND--NS 139
               A+SICDAAE WG FQ+ NHGVP  VL N   A   FF +  EEK +F K N   N+
Sbjct: 71  EPKVAESICDAAEKWGFFQIINHGVPIEVLENVKDATHSFFGLPAEEKRKFLKENSPTNN 130

Query: 140 ATYGTNFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEI 199
             +GT+F    EKALE                        D+++EY+K SE V+KR++E 
Sbjct: 131 VRFGTSFSPEAEKALEWKDYLSLFYVSEHEASTLWPLVCKDQVLEYMKRSELVIKRLLET 190

Query: 200 LMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGG 259
           LM++L V EIDE K SLLMGSKRI LNYYPICPNP L +GVGRHSDVSTLT+LLQD +GG
Sbjct: 191 LMKRLNVKEIDEIKESLLMGSKRINLNYYPICPNPELTVGVGRHSDVSTLTILLQDDIGG 250

Query: 260 LHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIF 319
           L+VR     + +W+H SP+ GSLVINIGDALQIMSNGR+KS+EH V ANGS NRIS+PIF
Sbjct: 251 LYVR---GNNDSWIHVSPISGSLVINIGDALQIMSNGRYKSIEHRVVANGSKNRISVPIF 310

Query: 320 VNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           VNP P  +IGP  EVLA G+K  YKQVLYSDYV+HF+N  H GK ++EFAKI
Sbjct: 311 VNPRPGDMIGPFQEVLAGGEKAAYKQVLYSDYVKHFFNKAHDGKQTVEFAKI 358

[Close]

BLAST of Moc02g00570 vs. ExPASy TrEMBL
Match: A0A2N9J402 (Fe2OG dioxygenase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60099 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 8.3e-103
Identity = 203/352 (57.67%), Postives = 245/352 (69.60%), Query Frame = 0


Query: 20  EEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV---SMLPIIDMSH-- 79
           E  D TDF ++KGNGVK LS+MGL  LP Q+IQP EER  +MS++     +PIIDMS+  
Sbjct: 11  ESSDITDFVVSKGNGVKGLSEMGLKTLPNQYIQPLEERL-SMSNIMPLESIPIIDMSNWD 70

Query: 80  --DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLND--NS 139
               A+SICDAAE WG FQ+ NHGVP  VL N   A   FF +  EEK +F K N   N+
Sbjct: 71  EPKVAESICDAAEKWGFFQIINHGVPIEVLENVKDATHSFFGLPTEEKRKFLKENSPTNN 130

Query: 140 ATYGTNFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEI 199
             +GT+F    EKALE                        D+++EY+K SE V+KR++E 
Sbjct: 131 VRFGTSFSPEAEKALEWKDYLSLFYVSEHEASTLWPLVCKDQVLEYMKRSELVIKRLLET 190

Query: 200 LMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGG 259
           LM++L V EIDE K SLLMGSKRI LNYYPICPNP L +GVGRHSDVSTLT+LLQD +GG
Sbjct: 191 LMKRLNVKEIDEIKESLLMGSKRINLNYYPICPNPELTVGVGRHSDVSTLTILLQDDIGG 250

Query: 260 LHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIF 319
           L+VR     + +W+H SP+ GSLVINIGDALQIMSNGR+KS+EH V ANGS NRIS+PIF
Sbjct: 251 LYVR---GNNDSWIHVSPISGSLVINIGDALQIMSNGRYKSIEHRVVANGSKNRISVPIF 310

Query: 320 VNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           VNP P  +IGP  EVLA G+K  YKQVLYSDYV+HF+N  H GK ++EFAKI
Sbjct: 311 VNPRPGDMIGPFQEVLAGGEKAAYKQVLYSDYVKHFFNKAHDGKQTVEFAKI 358

[Close]

BLAST of Moc02g00570 vs. ExPASy TrEMBL
Match: A0A6A4LYV6 (Fe2OG dioxygenase domain-containing protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_05761 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 4.1e-102
Identity = 198/364 (54.40%), Postives = 252/364 (69.23%), Query Frame = 0


Query: 7   MAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV-- 66
           MAP  +  + ++ + +D T F + +GNGVK LS++GL  LP Q+IQP EER  +MS +  
Sbjct: 1   MAPSLSLISDSDSDSLDVTKFAVYQGNGVKGLSELGLKTLPKQYIQPVEERMSSMSHIEX 60

Query: 67  SMLPIIDMSH----DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEK 126
             +PIIDM++      AD+ICDAAE WG FQ+ NHGVP  VL +   A  RFF +  EEK
Sbjct: 61  ESIPIIDMANWDDPKVADAICDAAEKWGFFQIINHGVPIEVLEDVKTATHRFFGLPAEEK 120

Query: 127 SRFFKLND--NSATYGTNFGTSPEKALE------------------------DEMVEYVK 186
            ++ K N   N+  YGT+F    EKALE                        D+ +E+++
Sbjct: 121 GKYSKENSCTNNVRYGTSFSPQAEKALEWKDYLSLFYVSDGEAASRWPSQCKDQALEFMR 180

Query: 187 SSEGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVS 246
            S+  +KR++E L ++L V E+DET ++LLMGSKRI LNYYPICPNP L +GVGRHSDVS
Sbjct: 181 RSDCAIKRLLEALTKRLNVKEMDETHQTLLMGSKRINLNYYPICPNPELTVGVGRHSDVS 240

Query: 247 TLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAA 306
           TLT+LLQD +GGL+VR+    D TW+H  P+ GSLVINIGDALQIMSNGR+KSVEH V A
Sbjct: 241 TLTILLQDEIGGLYVRKLDDED-TWIHVPPISGSLVINIGDALQIMSNGRYKSVEHRVMA 300

Query: 307 NGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIE 339
           NGS NRIS+PIFVNP PSAIIGPL EVL  G+KP YKQVLYSDYV++F+   H GK+++E
Sbjct: 301 NGSKNRISVPIFVNPXPSAIIGPLPEVLEGGEKPVYKQVLYSDYVKYFFRKGHDGKDTVE 360

[Close]

BLAST of Moc02g00570 vs. ExPASy TrEMBL
Match: A0A7N2QX50 (Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.2e-101
Identity = 200/352 (56.82%), Postives = 246/352 (69.89%), Query Frame = 0


Query: 20  EEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSV---SMLPIIDMSH-- 79
           E  + TDF +T  NGVK LS+MGL  LP Q+IQP EER  T+S++     +PIIDMS+  
Sbjct: 11  ESSNITDFVVTNRNGVKGLSEMGLKTLPKQYIQPVEERI-TVSNILPQESIPIIDMSNWD 70

Query: 80  --DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN--DNS 139
               ++SICDAAE WG FQ+ NHGVP  VL N   A  RFF++  EEKS+F K N   NS
Sbjct: 71  EPKVSESICDAAEKWGFFQIINHGVPIEVLENVKDATHRFFNLPAEEKSKFSKENSPSNS 130

Query: 140 ATYGTNFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEI 199
             +GT+F    EKALE                        D+++EY++ SE V++R++E 
Sbjct: 131 VRFGTSFSPEAEKALEWKDYLSMFYVSEDEASALWPSACKDQVLEYMRGSEPVIQRLLEA 190

Query: 200 LMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGG 259
           LM+++ V EIDETK+SLLMGSKRI LNYYPICPNP L +G+GRHSDVSTLT+LLQD +GG
Sbjct: 191 LMKRINVKEIDETKKSLLMGSKRINLNYYPICPNPELTVGIGRHSDVSTLTILLQDDIGG 250

Query: 260 LHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIF 319
           L+VR     + +WVH  P+ GSLVIN+GDALQIMSNGR+KS+EH V ANGS NRIS+PIF
Sbjct: 251 LYVR---GNNDSWVHVPPISGSLVINVGDALQIMSNGRYKSIEHRVVANGSKNRISVPIF 310

Query: 320 VNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           VNP P  +IGP  EVLA G+K  YKQVLYS+YVRHFY   H GK +IEFAKI
Sbjct: 311 VNPRPDDMIGPFPEVLARGEKALYKQVLYSNYVRHFYKKAHDGKKTIEFAKI 358

[Close]

BLAST of Moc02g00570 vs. TAIR 10
Match: AT3G13610.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 4.3e-96
Identity = 187/365 (51.23%), Postives = 242/365 (66.30%), Query Frame = 0


Query: 7   MAPRSASGTGNEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTM---SS 66
           MAP   +   +   EV  TDF + KGNGVK LS+ G+ ALP Q+IQP EER        +
Sbjct: 1   MAPTLLTTQFSNPAEV--TDFVVYKGNGVKGLSETGIKALPEQYIQPLEERLINKFVNET 60

Query: 67  VSMLPIIDMSHD----AADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEE 126
              +P+IDMS+      A+++CDAAE WG FQV NHGVP  VL +  AA  +FF++  EE
Sbjct: 61  DEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEE 120

Query: 127 KSRFFKLNDNSAT--YGTNFGTSPEKALE------------------------DEMVEYV 186
           K +F K N  S T  +GT+F    E+ALE                        +E +EY+
Sbjct: 121 KRKFTKENSLSTTVRFGTSFSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNETLEYI 180

Query: 187 KSSEGVVKRVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDV 246
             S+ +V+R++E L + L V E+DETK SL MGS R+ LNYYPICPNP L +GVGRHSDV
Sbjct: 181 NKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGRHSDV 240

Query: 247 STLTVLLQDHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVA 306
           S+LT+LLQD +GGLHVR    G+  WVH  PV GS VINIGDA+QIMSNG +KSVEH V 
Sbjct: 241 SSLTILLQDQIGGLHVRSLASGN--WVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHRVL 300

Query: 307 ANGSSNRISIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSI 339
           ANG +NRIS+PIFVNP+P ++IGPL EV+A G++P Y+ VLYSDYV++F+   H GK ++
Sbjct: 301 ANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKKTV 360

[Close]

BLAST of Moc02g00570 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.3e-92
Identity = 177/348 (50.86%), Postives = 225/348 (64.66%), Query Frame = 0


Query: 25  TDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTM----SSVSMLPIIDMSH----DA 84
           T+F + +GNGVK LS+ G+  LP Q+IQP EER         S   +P+ID+S+      
Sbjct: 16  TNFVVHEGNGVKGLSETGIKVLPDQYIQPFEERLINFHVKEDSDESIPVIDISNLDEKSV 75

Query: 85  ADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFF--KLNDNSATYG 144
           + ++CDAAE WG FQV NHGV   VL N   A  RFF +  EEK +F   K    +  +G
Sbjct: 76  SKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRKFSREKSLSTNVRFG 135

Query: 145 TNFGTSPEKALE------------------------DEMVEYVKSSEGVVKRVVEILMEK 204
           T+F    EKALE                         E +EY+  ++ +VK+++  L E 
Sbjct: 136 TSFSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRSETLEYMNETKPLVKKLLRFLGEN 195

Query: 205 LGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGLHVR 264
           L V E+D+TK S  MGS RI LNYYPICPNP L +GVGRHSDVS+LT+LLQD +GGLHVR
Sbjct: 196 LNVKELDKTKESFFMGSTRINLNYYPICPNPELTVGVGRHSDVSSLTILLQDEIGGLHVR 255

Query: 265 RPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFVNPE 324
             T G   WVH  P+ GSLVINIGDA+QIMSNGR+KSVEH V ANGS NRIS+PIFV+P+
Sbjct: 256 SLTTG--RWVHVPPISGSLVINIGDAMQIMSNGRYKSVEHRVLANGSYNRISVPIFVSPK 315

Query: 325 PSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           P ++IGPL EV+  G+KP YK +LY+DYV+HF+   H GK +I+FA I
Sbjct: 316 PESVIGPLLEVIENGEKPVYKDILYTDYVKHFFRKAHDGKKTIDFANI 361

[Close]

BLAST of Moc02g00570 vs. TAIR 10
Match: AT3G12900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.7e-68
Identity = 145/356 (40.73%), Postives = 205/356 (57.58%), Query Frame = 0


Query: 17  NEEEEVDETDFGITKGNGVKVLSQMGLTALPLQFIQPPEERFRTMSSVS--MLPIIDMSH 76
           N E++    +F + +GNGVK +   GL+++P  F+QP  ER  T  +++      ID+S+
Sbjct: 4   NFEDQTTLFNFVVREGNGVKGMIDSGLSSVPRPFVQPLSERIPTQKALTCEATQPIDLSN 63

Query: 77  -------DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLN 136
                  + A  I +AAE  G FQV NHGV   +L    ++A  FF  +PEEKS + K  
Sbjct: 64  LDGPQHKEVAKQIVEAAETLGFFQVVNHGVSVELLELLKSSAHEFFAQAPEEKSMYLKEV 123

Query: 137 DNS--ATYGTNFGTSPEKALE------------------------DEMVEYVKSSEGVVK 196
             S    YGT+F    EKA+E                        +  +E++ SS  +VK
Sbjct: 124 SPSKLVKYGTSFVPDKEKAIEWKDYVSMLYTNDSEALQHWPQPCREVALEFLNSSMEMVK 183

Query: 197 RVVEILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQ 256
            VV ILME +GV  ++E K + LMG+K + +NYYP CP+P L +GVGRHSD+  LTVLLQ
Sbjct: 184 NVVNILMENVGVT-LEEEKMNGLMGTKMVNMNYYPTCPSPELTVGVGRHSDMGMLTVLLQ 243

Query: 257 DHVGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRI 316
           D +GGL+V+     +  W    PV G+LVINIGD LQI+SNG++KS EH V      +R+
Sbjct: 244 DGIGGLYVKL---DNGEWAEIPPVHGALVINIGDTLQILSNGKYKSAEHRVRTTNIGSRV 303

Query: 317 SIPIFVNPEPSAIIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAK 338
           S+PIF  P PS  +GPL EV+       YK+ L+ DY+ +F+  PH GK S++FA+
Sbjct: 304 SVPIFTAPNPSQKVGPLPEVVKRDGVARYKEFLFQDYMNNFFGQPHDGKKSLDFAR 355

[Close]

BLAST of Moc02g00570 vs. TAIR 10
Match: AT5G12270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.6e-66
Identity = 135/356 (37.92%), Postives = 210/356 (58.99%), Query Frame = 0


Query: 20  EEVDETDFGITKGNGVK-VLSQMGLTALPLQFIQPPEERFRTMSSV--SMLPIIDMSH-- 79
           +E++  DF + + NGVK ++  + LT LP  +IQPP+ERF +   +  S +P+ID+S+  
Sbjct: 6   DEIEVRDFVVNQKNGVKGLVDFLTLTTLPSPYIQPPQERFTSDKILLGSPVPVIDVSNWN 65

Query: 80  --DAADSICDAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLNDNSAT 139
               A  IC AA   G+FQ+ NHG+ P      +AAAR FF++  EE+ R+++ +  S T
Sbjct: 66  EPHVAREICHAASKLGLFQIVNHGIAPAEFKGVIAAARGFFELPAEERRRYWRGSSVSET 125

Query: 140 --YGTNFGTSPEKALE-------------------------DEMVEYVKSSEGVVKRVVE 199
               T+F    E  LE                         ++++++ K  + + +R++ 
Sbjct: 126 AWLTTSFNPCIESVLEWRDFLKFEYLPQRHDFAATWPSVCKEQVIDHFKRIKPITERILN 185

Query: 200 ILMEKLGVVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDH-- 259
           IL+  L  + IDE+ +  LMG+ R+  NYYP CP P LA+G GRHSD++TLT+LLQ+   
Sbjct: 186 ILINNLNTI-IDESNKETLMGTMRMNFNYYPKCPEPSLAIGTGRHSDINTLTLLLQEDGV 245

Query: 260 VGGLHVRRPTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISI 319
           +  L+ R    GD  W+H  P+PG++V+NIGD LQI+SN R++SVEH V  N   +R+SI
Sbjct: 246 LSSLYARATEDGD-KWIHVPPIPGAIVVNIGDVLQILSNDRYRSVEHCVVVNKYCSRVSI 305

Query: 320 PIFVNPEPSAIIGPLSEVL-ATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
           P+F  P   ++I PL EVL    +   Y++++YSDY++ F+  PH GK +IE  K+
Sbjct: 306 PVFCGPVHDSVIEPLPEVLDKNNEMARYRKIVYSDYLKFFFGRPHDGKKTIESIKL 359

[Close]

BLAST of Moc02g00570 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 121/350 (34.57%), Postives = 171/350 (48.86%), Query Frame = 0


Query: 34  GVKVLSQMGLTALPLQFIQPPEERFR---TMSSVSMLPIIDMSHDAADSIC--------- 93
           GVK L   G+T +P  F  PP          SS   +P ID+     DSI          
Sbjct: 26  GVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGTDSITRRSLVEKIG 85

Query: 94  DAAENWGVFQVTNHGVPPRVLHNAMAAARRFFDMSPEEKSRFFKLNDNS-ATYGTNFG-- 153
           DAAE WG FQV NHG+P  VL   +   R F +   E K  F+  +  S   Y +NF   
Sbjct: 86  DAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDPASKMVYSSNFDLF 145

Query: 154 --------------TSPEKALEDE--------MVEYVKSSEGVVKRVVEILMEKLG---- 213
                         T+P+    ++        M+EY K    + K + E+L E LG    
Sbjct: 146 SSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLLFELLSEALGLNTN 205

Query: 214 -VVEIDETKRSLLMGSKRIKLNYYPICPNPHLALGVGRHSDVSTLTVLLQDHVGGLHVRR 273
            + ++D T   LL+G      +YYP CP P L LG+ +HSD S LT+LLQDH+GGL V  
Sbjct: 206 HLKDMDCTNSLLLLG------HYYPPCPQPDLTLGLTKHSDNSFLTILLQDHIGGLQVLH 265

Query: 274 PTHGDATWVHASPVPGSLVINIGDALQIMSNGRFKSVEHFVAANGSSNRISIPIFVNPEP 333
               D  WV   PVPG+LV+N+GD LQ+++N +F SVEH V AN +  RIS+  F +   
Sbjct: 266 ----DQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVAGPRISVACFFSSYL 325

Query: 334 SA---IIGPLSEVLATGQKPEYKQVLYSDYVRHFYNNPHHGKNSIEFAKI 339
            A   + GP+ E+L+    P Y+    ++Y + + +    G + + + KI
Sbjct: 326 MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGLLYLKI 365

The following BLAST results are available for this feature:

BLAST of Moc02g00570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153808.1	1.7e-187	93.37	LOW QUALITY PROTEIN: feruloyl CoA ortho-hydroxylase 1 [Momordica charantia]	[more]
KAE9462342.1	8.5e-102	54.40	hypothetical protein C3L33_05761, partial [Rhododendron williamsianum]	[more]
KAF3948390.1	1.1e-101	56.44	hypothetical protein CMV_025603 [Castanea mollissima]	[more]
XP_030952583.1	2.5e-101	56.82	feruloyl CoA ortho-hydroxylase F6H1-3-like [Quercus lobata]	[more]
XP_030952454.1	3.2e-101	56.70	feruloyl CoA ortho-hydroxylase F6H1-3-like isoform X2 [Quercus lobata]	[more]

BLAST of Moc02g00570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G9M9M1	1.9e-96	52.45	Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 ...	[more]
G9M9M2	4.2e-96	52.45	Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 ...	[more]
G9M9M0	3.6e-95	51.59	Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 ...	[more]
Q9LHN8	6.1e-95	51.23	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
W5QJZ5	8.0e-95	50.82	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox4 OS=Ruta gra...	[more]

BLAST of Moc02g00570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIJ1	8.3e-188	93.37	LOW QUALITY PROTEIN: feruloyl CoA ortho-hydroxylase 1 OS=Momordica charantia OX=...	[more]
A0A2N9GN50	8.3e-103	57.67	Fe2OG dioxygenase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_L...	[more]
A0A2N9J402	8.3e-103	57.67	Fe2OG dioxygenase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_L...	[more]
A0A6A4LYV6	4.1e-102	54.40	Fe2OG dioxygenase domain-containing protein (Fragment) OS=Rhododendron williamsi...	[more]
A0A7N2QX50	1.2e-101	56.82	Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of Moc02g00570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13610.1	4.3e-96	51.23	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G55290.1	1.3e-92	50.86	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G12900.1	1.7e-68	40.73	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G12270.1	3.6e-66	37.92	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G06620.1	1.6e-45	34.57	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 192..289 e-value: 1.0E-23 score: 83.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 67..137 e-value: 8.3E-15 score: 55.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 24..321 e-value: 7.1E-79 score: 267.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 23..146
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 145..329
None	No IPR available	PANTHER	PTHR10209:SF713	FERULOYL COA ORTHO-HYDROXYLASE 2	coord: 23..146
None	No IPR available	PANTHER	PTHR10209:SF713	FERULOYL COA ORTHO-HYDROXYLASE 2	coord: 145..329
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 34..329
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 186..289 score: 12.655376

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g00570.1	Moc02g00570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity