Moc02g00550 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g00550
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUbiquitin-conjugating enzyme family protein
Locationchr2: 455762 .. 458013 (+)
RNA-Seq ExpressionMoc02g00550
SyntenyMoc02g00550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGAAATCGAGAGCAGCGTCCGCAATTGCCTCTCGTAGGTGGGGGGCGACCGTCTTCCTCATCCTCCTTAGCACCTCCAACTTCATGGGCGTCCACAACTTCGGTTTCTGCTTCAGGCAAGAGAATTCAAAGAGAAATGGCTGAGTTTAATTCCGACCCACCTTCCCATTGCTCCGCTGGCCCAAAGGGTGACAATCTCTACCACTGGTTCGCTACCATCTTTGGCCCTCCTGGTAACTGCTACCCTTCTACTTCTTTCACTTTTAGAATTTTGATGATGCCGTTTCGGTTTTATCGCTGTTCATACGATATCTGGTTTCCCCTCTTTTGGTCATGCTTTCCCGTTCTTCTTTTACTCGCTCGCTTCCGCCAAAGATTTCTGAATATTTGGCGTGTTTATGGACAAGGTTTTAACTTTTCTGCCAGATTTTTATGATTAAAGTGTCTTCAATTGGGCATGGCCGTTAGGATTTCACTTTCCTGCTTTGTTAGTAGCTTTACTTCTTTCGAGCAGTTATTTATCATCTTCGGCACTCTCTCATTTGTTACAATTCTGAACGGATGCTCGTTGAAACTCAGATGGGGTTTTATTACCTGATTGCTAAACATATTCTTTGCCCTTTGCTACCTATAGTTTAGTTACAATTCTGAAGAAAGTGATTAGGGTAGTTTCCAGGCATGTAAGTTATCAAACTGAGTAAATGGAGTCAGAGTTGGAGAGAAGCTAATTTTAGATCAAAGTTGTCGAACCCACAATCTTCCAGATGGAACTTGAGCAAGAAACTAAGTATGCCCCAAAGAAATACACAGTATGCTGCCATGGACTTCCTACCATGCTCGTGCTCAAACCTAATCCGCAACTATTTAACTAATATCTCTGAAAACAATACACAAAATGATCAGGGTAGAAGATGCTCACTGATGGAGTTCTATTACCCAATTGTTTGATGCTGAAGAACGAACCAGTCTATTGGACATTTGTTGCACAAAACTCAGAAGGATTTGGAAGAATGAACTCTAACTAAATAGTCACACTACACTAATATATACAAATTCTTGAATGGATGATTGCACAACCATTGGTGTCGAGGAATTTCAGCATGTAACTTGCTGAATGAAGGGAGAGTCGTGCGTTTCTGTTGGACACAATAAAGTATAATACTACCTTTCTCCTCTGCATTAAGTGAATTGTTTTTAGTGAAACCGAGTGTTTGATAAAATAAACTTCCTCATCAAAGTCTTACTGAAGAAATAGTCAGGCAAGAGAAATAAAAGTTAAGCCTATGTCCATCGTGTCTGATATTAGTTTATTAGACAAGAAACAATGCAATGAAGGGAACGGAAAATGATGCTCAAGTGATGTTTAATGGCATAGATTATTTGATAGGCAGGAAAAGCTTAGGTTAAAAAGTAAACGACTTCATAAATCTAGTACCAGCGAGCTCCTTCCTCTTCCTCTCTTTGTCTCAGGTTTCTTTTTCTCCTATAAGTAGTAGACATGAAAGTATAAGTCATTTGTCTATAGAGTTGTATTCACTTTGATTGTTTGATGACTGAGTATAAATACCATCTCCAATAGATGTGTTGGTGGCAGGTTTTTCATTTTGCATTCAAGGTTGTGTTTAGCTGTTCAATTTTACATAGCTCTTTCCGGCAGTAGTAACCTTGACTAATATCTTTGTCTTGTTTTTAGGAACACCATATGAAGGTGGTATATTTTTTCTTGACATACTTTTTCCATGTGATTATCCATTCTATCCTCCAAAGGTACTCTCACTTACATCCTTAAAATTTAGGATACAAAATCTAAAATTTCTCTTGCCTTCATCACGAGCATGGGATGATCATTCTTGACCTAATGCACACGGTTCTGAGTTTTATATCGTTTGTTGATTTATTAAGAAGGATCAGATCTGCAGGTTGTATTTAAAACCCGAATCTACCACTGCAATGTGGATTCCGCTGGTAATGTAAGTTTGGACATTCTGAAGGAGAACTGGAGTCCAGCACTGACAATCACAAAGGTGTTAATTGCAGTGAAGTCGATTTTTGAAAATCCAAACCCTTGTGAGTCACCCTTCTTTTTCTAAGGATTGTGCTCTGTCGAAGATGCAGCTGTTACAAATGCTTACTTTGCCCACTTTTGCAGATGATCCAAACGCACCTGGCGTTGCTCACTTGTACTTAACCGATAGATGTAAGCACGACGAGATAGCTGCTGAGTGGACACTACGTTTTGCCAAGTGA

mRNA sequence

ATGGAGGGAAATCGAGAGCAGCGTCCGCAATTGCCTCTCGTAGGTGGGGGGCGACCGTCTTCCTCATCCTCCTTAGCACCTCCAACTTCATGGGCGTCCACAACTTCGGTTTCTGCTTCAGGCAAGAGAATTCAAAGAGAAATGGCTGAGTTTAATTCCGACCCACCTTCCCATTGCTCCGCTGGCCCAAAGGGTGACAATCTCTACCACTGGTTCGCTACCATCTTTGGCCCTCCTGGAACACCATATGAAGGTGGTATATTTTTTCTTGACATACTTTTTCCATGTGATTATCCATTCTATCCTCCAAAGGTTGTATTTAAAACCCGAATCTACCACTGCAATGTGGATTCCGCTGGTAATGTAAGTTTGGACATTCTGAAGGAGAACTGGAGTCCAGCACTGACAATCACAAAGGTGTTAATTGCAGTGAAGTCGATTTTTGAAAATCCAAACCCTTATGATCCAAACGCACCTGGCGTTGCTCACTTGTACTTAACCGATAGATGTAAGCACGACGAGATAGCTGCTGAGTGGACACTACGTTTTGCCAAGTGA

Coding sequence (CDS)

ATGGAGGGAAATCGAGAGCAGCGTCCGCAATTGCCTCTCGTAGGTGGGGGGCGACCGTCTTCCTCATCCTCCTTAGCACCTCCAACTTCATGGGCGTCCACAACTTCGGTTTCTGCTTCAGGCAAGAGAATTCAAAGAGAAATGGCTGAGTTTAATTCCGACCCACCTTCCCATTGCTCCGCTGGCCCAAAGGGTGACAATCTCTACCACTGGTTCGCTACCATCTTTGGCCCTCCTGGAACACCATATGAAGGTGGTATATTTTTTCTTGACATACTTTTTCCATGTGATTATCCATTCTATCCTCCAAAGGTTGTATTTAAAACCCGAATCTACCACTGCAATGTGGATTCCGCTGGTAATGTAAGTTTGGACATTCTGAAGGAGAACTGGAGTCCAGCACTGACAATCACAAAGGTGTTAATTGCAGTGAAGTCGATTTTTGAAAATCCAAACCCTTATGATCCAAACGCACCTGGCGTTGCTCACTTGTACTTAACCGATAGATGTAAGCACGACGAGATAGCTGCTGAGTGGACACTACGTTTTGCCAAGTGA

Protein sequence

MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFAK
Homology
BLAST of Moc02g00550 vs. NCBI nr
Match: XP_022153725.1 (constitutive photomorphogenesis protein 10 [Momordica charantia])

HSP 1 Score: 391.7 bits (1005), Expect = 3.4e-105
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS
Sbjct: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT
Sbjct: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of Moc02g00550 vs. NCBI nr
Match: XP_038902201.1 (constitutive photomorphogenesis protein 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 352.1 bits (902), Expect = 3.0e-93
Identity = 167/185 (90.27%), Postives = 171/185 (92.43%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+ RPQLPL GGGR SSS+SLAPPTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 1   MVGNRDHRPQLPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDSAG
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSAG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           N SLDILK+NWSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 121 NASLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of Moc02g00550 vs. NCBI nr
Match: XP_038902200.1 (constitutive photomorphogenesis protein 10 isoform X1 [Benincasa hispida])

HSP 1 Score: 350.1 bits (897), Expect = 1.1e-92
Identity = 166/184 (90.22%), Postives = 170/184 (92.39%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+ RPQLPL GGGR SSS+SLAPPTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 1   MVGNRDHRPQLPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDSAG
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSAG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           N SLDILK+NWSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 121 NASLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFA 185
           LRFA
Sbjct: 181 LRFA 184

BLAST of Moc02g00550 vs. NCBI nr
Match: XP_022943567.1 (constitutive photomorphogenesis protein 10 [Cucurbita moschata] >XP_022986179.1 constitutive photomorphogenesis protein 10 [Cucurbita maxima] >XP_023512726.1 constitutive photomorphogenesis protein 10 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7010225.1 Constitutive photomorphogenesis protein 10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348.2 bits (892), Expect = 4.3e-92
Identity = 165/185 (89.19%), Postives = 171/185 (92.43%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+ RPQ PL GGGR SSS+SLAPPTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 12  MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 71

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDS+G
Sbjct: 72  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 131

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILK++WSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 132 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 191

Query: 181 LRFAK 186
           LRFAK
Sbjct: 192 LRFAK 196

BLAST of Moc02g00550 vs. NCBI nr
Match: KAG6570333.1 (Constitutive photomorphogenesis protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 348.2 bits (892), Expect = 4.3e-92
Identity = 165/185 (89.19%), Postives = 171/185 (92.43%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+ RPQ PL GGGR SSS+SLAPPTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 1   MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDS+G
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILK++WSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 121 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of Moc02g00550 vs. ExPASy Swiss-Prot
Match: Q9LJD7 (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana OX=3702 GN=COP10 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-68
Identity = 119/157 (75.80%), Postives = 135/157 (85.99%), Query Frame = 0

Query: 29  TSWASTTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIF 88
           +SW + TSVSASGKRIQREMAE N DPP  CSAGPKGDNLYHW ATI GP GTPYEGGIF
Sbjct: 26  SSWVAKTSVSASGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIF 85

Query: 89  FLDILFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIF 148
           FLDI+FP DYPF PPK+VFKTRIYHCNVD+AG++S++IL+++WSPALTITKVL A++SIF
Sbjct: 86  FLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIF 145

Query: 149 ENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFAK 186
             P PY P  P +A LYLTDR KHDE+A EWTLRFAK
Sbjct: 146 LKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFAK 182

BLAST of Moc02g00550 vs. ExPASy Swiss-Prot
Match: Q2T9X7 (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus OX=9913 GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.2e-53
Identity = 92/152 (60.53%), Postives = 117/152 (76.97%), Query Frame = 0

Query: 33  STTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI 92
           +T  +S S KRIQ+E+AE   DPP +CSAGPKGDN+Y W +TI GPPG+ YEGG+FFLDI
Sbjct: 55  TTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDI 114

Query: 93  LFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPN 152
            F  DYPF PPKV F+TRIYHCN++S G + LDILK+NWSPALTI+KVL+++ S+  + N
Sbjct: 115 TFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN 174

Query: 153 PYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFA 185
           P DP    +A  YLT+R +HD IA +WT R+A
Sbjct: 175 PADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

BLAST of Moc02g00550 vs. ExPASy Swiss-Prot
Match: Q969T4 (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens OX=9606 GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.2e-53
Identity = 92/152 (60.53%), Postives = 117/152 (76.97%), Query Frame = 0

Query: 33  STTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI 92
           +T  +S S KRIQ+E+AE   DPP +CSAGPKGDN+Y W +TI GPPG+ YEGG+FFLDI
Sbjct: 55  TTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDI 114

Query: 93  LFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPN 152
            F  DYPF PPKV F+TRIYHCN++S G + LDILK+NWSPALTI+KVL+++ S+  + N
Sbjct: 115 TFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN 174

Query: 153 PYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFA 185
           P DP    +A  YLT+R +HD IA +WT R+A
Sbjct: 175 PADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

BLAST of Moc02g00550 vs. ExPASy Swiss-Prot
Match: P52483 (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus OX=10090 GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 9.2e-53
Identity = 92/152 (60.53%), Postives = 117/152 (76.97%), Query Frame = 0

Query: 33  STTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI 92
           +T  +S S KRIQ+E+AE   DPP +CSAGPKGDN+Y W +TI GPPG+ YEGG+FFLDI
Sbjct: 55  TTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDI 114

Query: 93  LFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPN 152
            F  DYPF PPKV F+TRIYHCN++S G + LDILK+NWSPALTI+KVL+++ S+  + N
Sbjct: 115 TFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN 174

Query: 153 PYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFA 185
           P DP    +A  YLT+R +HD IA +WT R+A
Sbjct: 175 PADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

BLAST of Moc02g00550 vs. ExPASy Swiss-Prot
Match: Q96LR5 (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens OX=9606 GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.3e-51
Identity = 89/152 (58.55%), Postives = 116/152 (76.32%), Query Frame = 0

Query: 33  STTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI 92
           +   +S S KRIQ+E+AE   DPP +CSAGPKGDN+Y W +TI GPPG+ YEGG+FFLDI
Sbjct: 49  TAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDI 108

Query: 93  LFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPN 152
            F  DYPF PPKV F+TRIYHCN++S G + LDILK+NWSPALTI+KVL+++ S+  + N
Sbjct: 109 TFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN 168

Query: 153 PYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFA 185
           P DP    +A  Y+T+R +HD +A +WT R+A
Sbjct: 169 PADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200

BLAST of Moc02g00550 vs. ExPASy TrEMBL
Match: A0A6J1DHM4 (constitutive photomorphogenesis protein 10 OS=Momordica charantia OX=3673 GN=LOC111021177 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.7e-105
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS
Sbjct: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT
Sbjct: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of Moc02g00550 vs. ExPASy TrEMBL
Match: A0A6J1JFC7 (constitutive photomorphogenesis protein 10 OS=Cucurbita maxima OX=3661 GN=LOC111483999 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.1e-92
Identity = 165/185 (89.19%), Postives = 171/185 (92.43%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+ RPQ PL GGGR SSS+SLAPPTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 12  MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 71

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDS+G
Sbjct: 72  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 131

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILK++WSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 132 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 191

Query: 181 LRFAK 186
           LRFAK
Sbjct: 192 LRFAK 196

BLAST of Moc02g00550 vs. ExPASy TrEMBL
Match: A0A6J1FTD1 (constitutive photomorphogenesis protein 10 OS=Cucurbita moschata OX=3662 GN=LOC111448300 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.1e-92
Identity = 165/185 (89.19%), Postives = 171/185 (92.43%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+ RPQ PL GGGR SSS+SLAPPTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 12  MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 71

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDS+G
Sbjct: 72  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 131

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILK++WSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 132 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 191

Query: 181 LRFAK 186
           LRFAK
Sbjct: 192 LRFAK 196

BLAST of Moc02g00550 vs. ExPASy TrEMBL
Match: A0A5D3DDP3 (Constitutive photomorphogenesis protein 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00610 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.0e-91
Identity = 165/185 (89.19%), Postives = 169/185 (91.35%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+QRPQLP +GGGR SSS SLA PTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 1   MVGNRDQRPQLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGP GTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDS G
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILK+NWSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFVNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of Moc02g00550 vs. ExPASy TrEMBL
Match: A0A1S3BMW7 (constitutive photomorphogenesis protein 10 OS=Cucumis melo OX=3656 GN=LOC103491591 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.0e-91
Identity = 165/185 (89.19%), Postives = 169/185 (91.35%), Query Frame = 0

Query: 1   MEGNREQRPQLPLVGGGRPSSSSSLAPPTSWASTTSVSASGKRIQREMAEFNSDPPSHCS 60
           M GNR+QRPQLP +GGGR SSS SLA PTSWASTTSVSASGKRIQREM EFN DPPS CS
Sbjct: 1   MVGNRDQRPQLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFYPPKVVFKTRIYHCNVDSAG 120
           AGPKGDNLYHWFATIFGP GTPYEGGIFFLDI+FP DYPF PPKVVFKTRIYHCNVDS G
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWT 180
           NVSLDILK+NWSPALTI KVLIAVKSIF NPNPYDPNAPGVAHLYL DR KHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFVNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

BLAST of Moc02g00550 vs. TAIR 10
Match: AT3G13550.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.1e-69
Identity = 119/157 (75.80%), Postives = 135/157 (85.99%), Query Frame = 0

Query: 29  TSWASTTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIF 88
           +SW + TSVSASGKRIQREMAE N DPP  CSAGPKGDNLYHW ATI GP GTPYEGGIF
Sbjct: 26  SSWVAKTSVSASGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIF 85

Query: 89  FLDILFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIF 148
           FLDI+FP DYPF PPK+VFKTRIYHCNVD+AG++S++IL+++WSPALTITKVL A++SIF
Sbjct: 86  FLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIF 145

Query: 149 ENPNPYDPNAPGVAHLYLTDRCKHDEIAAEWTLRFAK 186
             P PY P  P +A LYLTDR KHDE+A EWTLRFAK
Sbjct: 146 LKPEPYSPALPVIARLYLTDREKHDEVAKEWTLRFAK 182

BLAST of Moc02g00550 vs. TAIR 10
Match: AT3G13550.2 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 211.5 bits (537), Expect = 5.9e-55
Identity = 96/125 (76.80%), Postives = 110/125 (88.00%), Query Frame = 0

Query: 29  TSWASTTSVSASGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIF 88
           +SW + TSVSASGKRIQREMAE N DPP  CSAGPKGDNLYHW ATI GP GTPYEGGIF
Sbjct: 26  SSWVAKTSVSASGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIF 85

Query: 89  FLDILFPCDYPFYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIF 148
           FLDI+FP DYPF PPK+VFKTRIYHCNVD+AG++S++IL+++WSPALTITKVL A++SIF
Sbjct: 86  FLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRDSWSPALTITKVLQAIRSIF 145

Query: 149 ENPNP 154
             P P
Sbjct: 146 LKPEP 150

BLAST of Moc02g00550 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 187.6 bits (475), Expect = 9.1e-48
Identity = 79/145 (54.48%), Postives = 109/145 (75.17%), Query Frame = 0

Query: 40  SGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYP 99
           + KRI +E+ +   DPPS+CSAGP  ++++HW ATI GPP +PY GG+F + I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 100 FYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAP 159
           F PPKV FKT++YH N++S G++ LDILKE WSPALTI+KVL+++ S+  +PNP DP  P
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 160 GVAHLYLTDRCKHDEIAAEWTLRFA 185
            +AH+Y TDR K++  A  WT ++A
Sbjct: 122 EIAHMYKTDRSKYESTARSWTQKYA 146

BLAST of Moc02g00550 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 187.6 bits (475), Expect = 9.1e-48
Identity = 79/145 (54.48%), Postives = 109/145 (75.17%), Query Frame = 0

Query: 40  SGKRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYP 99
           + KRI +E+ +   DPPS+CSAGP  ++++HW ATI GPP +PY GG+F + I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 100 FYPPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAP 159
           F PPKV FKT++YH N++S G++ LDILKE WSPALTI+KVL+++ S+  +PNP DP  P
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 160 GVAHLYLTDRCKHDEIAAEWTLRFA 185
            +AH+Y TDR K++  A  WT ++A
Sbjct: 122 EIAHMYKTDRSKYESTARSWTQKYA 146

BLAST of Moc02g00550 vs. TAIR 10
Match: AT2G16740.1 (ubiquitin-conjugating enzyme 29 )

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-46
Identity = 76/143 (53.15%), Postives = 109/143 (76.22%), Query Frame = 0

Query: 42  KRIQREMAEFNSDPPSHCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDILFPCDYPFY 101
           +RI +E+ E   DPP  CSAGP G++++HW ATI GP  +PY GG+F ++I FP DYPF 
Sbjct: 4   RRILKELKELQRDPPVSCSAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPFK 63

Query: 102 PPKVVFKTRIYHCNVDSAGNVSLDILKENWSPALTITKVLIAVKSIFENPNPYDPNAPGV 161
           PPKVVF+T+++H N++S GN+ LDILK+ WSPALTI+KVL+++ S+  +PNP DP  P +
Sbjct: 64  PPKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 123

Query: 162 AHLYLTDRCKHDEIAAEWTLRFA 185
           AH+Y TD+ K++ +A  WT ++A
Sbjct: 124 AHIYKTDKTKYEAMARSWTQKYA 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153725.13.4e-105100.00constitutive photomorphogenesis protein 10 [Momordica charantia][more]
XP_038902201.13.0e-9390.27constitutive photomorphogenesis protein 10 isoform X2 [Benincasa hispida][more]
XP_038902200.11.1e-9290.22constitutive photomorphogenesis protein 10 isoform X1 [Benincasa hispida][more]
XP_022943567.14.3e-9289.19constitutive photomorphogenesis protein 10 [Cucurbita moschata] >XP_022986179.1 ... [more]
KAG6570333.14.3e-9289.19Constitutive photomorphogenesis protein 10, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
Q9LJD71.6e-6875.80Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana OX=3702 GN=CO... [more]
Q2T9X79.2e-5360.53Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus OX=9913 GN=UBE2E3 PE=2 SV=1[more]
Q969T49.2e-5360.53Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens OX=9606 GN=UBE2E3 PE=1 SV=1[more]
P524839.2e-5360.53Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus OX=10090 GN=Ube2e3 PE=1 SV=2[more]
Q96LR52.3e-5158.55Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens OX=9606 GN=UBE2E2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DHM41.7e-105100.00constitutive photomorphogenesis protein 10 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1JFC72.1e-9289.19constitutive photomorphogenesis protein 10 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1FTD12.1e-9289.19constitutive photomorphogenesis protein 10 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5D3DDP33.0e-9189.19Constitutive photomorphogenesis protein 10 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BMW73.0e-9189.19constitutive photomorphogenesis protein 10 OS=Cucumis melo OX=3656 GN=LOC1034915... [more]
Match NameE-valueIdentityDescription
AT3G13550.11.1e-6975.80Ubiquitin-conjugating enzyme family protein [more]
AT3G13550.25.9e-5576.80Ubiquitin-conjugating enzyme family protein [more]
AT3G08690.19.1e-4854.48ubiquitin-conjugating enzyme 11 [more]
AT3G08690.29.1e-4854.48ubiquitin-conjugating enzyme 11 [more]
AT2G16740.11.0e-4653.15ubiquitin-conjugating enzyme 29 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 42..185
e-value: 2.6E-58
score: 209.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..39
NoneNo IPR availablePANTHERPTHR24068:SF62UBIQUITIN-CONJUGATING ENZYME E2 E2coord: 26..184
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 26..184
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 43..178
e-value: 7.3E-42
score: 142.3
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 39..185
score: 49.074703
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 42..180
e-value: 1.30474E-58
score: 178.165
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 30..185
e-value: 4.3E-58
score: 197.4
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 29..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g00550.1Moc02g00550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0010099 regulation of photomorphogenesis
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0061631 ubiquitin conjugating enzyme activity