Moc02g00250 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc02g00250 vs. NCBI nr
Match: XP_022153860.1 (pheophytinase, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 592.4 bits (1526), Expect = 2.5e-165
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MVLEQTGLHSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFY 60
           MVLEQTGLHSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFY
Sbjct: 1   MVLEQTGLHSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFY 60

Query: 61  TFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIK 120
           TFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIK
Sbjct: 61  TFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIK 120

Query: 121 KQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILN 180
           KQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILN
Sbjct: 121 KQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILN 180

Query: 181 PGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVE 240
           PGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVE
Sbjct: 181 PGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVE 240

Query: 241 DFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSASACTSSG 284
           DFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSASACTSSG
Sbjct: 241 DFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSASACTSSG 283

BLAST of Moc02g00250 vs. NCBI nr
Match: XP_022153858.1 (pheophytinase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 554.3 bits (1427), Expect = 7.5e-154
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 20  SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD 79
           SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD
Sbjct: 115 SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD 174

Query: 80  EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 139
           EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN
Sbjct: 175 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 234

Query: 140 TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 199
           TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG
Sbjct: 235 TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 294

Query: 200 GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL 259
           GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL
Sbjct: 295 GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL 354

Query: 260 VNPLVKSFVSRHATRSASACTSSG 284
           VNPLVKSFVSRHATRSASACTSSG
Sbjct: 355 VNPLVKSFVSRHATRSASACTSSG 378

BLAST of Moc02g00250 vs. NCBI nr
Match: XP_038901558.1 (pheophytinase, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 541.6 bits (1394), Expect = 5.0e-150
Identity = 257/274 (93.80%), Postives = 266/274 (97.08%), Query Frame = 0

Query: 1   MVLEQTGL-HSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESF 60
           MVLEQTGL HS FAS TT +SDHWRKNI  LAESHRVYALDLIGYGYSDKPNPDVVGE+F
Sbjct: 1   MVLEQTGLHHSIFASHTTNHSDHWRKNIPVLAESHRVYALDLIGYGYSDKPNPDVVGENF 60

Query: 61  YTFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHI 120
           YTFETWA+QLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHI
Sbjct: 61  YTFETWASQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHI 120

Query: 121 KKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIIL 180
           KKQPWYG+PLIRSFQ+LLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIIL
Sbjct: 121 KKQPWYGKPLIRSFQNLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIIL 180

Query: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESV 240
           NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLI WGDKDPWEPIELGR+Y +FESV
Sbjct: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIGWGDKDPWEPIELGRDYGNFESV 240

Query: 241 EDFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHAT 274
           EDFVVLPNVGHCPQDEAPHLVNPLV+SFVSRHA+
Sbjct: 241 EDFVVLPNVGHCPQDEAPHLVNPLVESFVSRHAS 274

BLAST of Moc02g00250 vs. NCBI nr
Match: XP_011653524.1 (pheophytinase, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 540.0 bits (1390), Expect = 1.5e-149
Identity = 255/277 (92.06%), Postives = 265/277 (95.67%), Query Frame = 0

Query: 1   MVLEQTGL-HSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESF 60
           MVLEQTGL HS FAS TT YSDHWRKNI  LA+SHRVYA+DLIGYGYSDKPNPD+VGE F
Sbjct: 1   MVLEQTGLHHSIFASHTTNYSDHWRKNIPVLAQSHRVYAIDLIGYGYSDKPNPDLVGEGF 60

Query: 61  YTFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHI 120
           YTFETWA+QLNDFCVDVVQD AFFICNSIGGVVGLQAA+MKPQICKGIVLLNISLRMLHI
Sbjct: 61  YTFETWASQLNDFCVDVVQDNAFFICNSIGGVVGLQAAIMKPQICKGIVLLNISLRMLHI 120

Query: 121 KKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIIL 180
           KKQPWYG+P IRSFQ+LLRNTALGKYFFR VATPESVKNILCQCYHDTSQVTDELVQIIL
Sbjct: 121 KKQPWYGKPFIRSFQNLLRNTALGKYFFRAVATPESVKNILCQCYHDTSQVTDELVQIIL 180

Query: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESV 240
           NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNY SF+SV
Sbjct: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYASFDSV 240

Query: 241 EDFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSA 277
           E+FVVLPNVGHCPQDEAPHLVNPLV+SFVS HATRSA
Sbjct: 241 EEFVVLPNVGHCPQDEAPHLVNPLVESFVSGHATRSA 277

BLAST of Moc02g00250 vs. NCBI nr
Match: XP_008449882.1 (PREDICTED: pheophytinase, chloroplastic isoform X4 [Cucumis melo] >XP_008449883.1 PREDICTED: pheophytinase, chloroplastic isoform X4 [Cucumis melo])

HSP 1 Score: 537.0 bits (1382), Expect = 1.2e-148
Identity = 253/277 (91.34%), Postives = 265/277 (95.67%), Query Frame = 0

Query: 1   MVLEQTGL-HSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESF 60
           MVLEQTGL HS FAS TT YSDHWRKNI  LA+SHRVYA+DLIGYGYSDKPNP++VGE F
Sbjct: 1   MVLEQTGLHHSIFASHTTNYSDHWRKNIPVLAQSHRVYAIDLIGYGYSDKPNPELVGEGF 60

Query: 61  YTFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHI 120
           YTFETWA+QLNDFCVDVVQD+AFFICNSIGGVVGLQAA+MKPQIC GIVLLNISLRMLHI
Sbjct: 61  YTFETWASQLNDFCVDVVQDDAFFICNSIGGVVGLQAAIMKPQICNGIVLLNISLRMLHI 120

Query: 121 KKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIIL 180
           KKQPWYG+P IRSFQ+LLRNTALGKYFFR VATPESVKNILCQCYHDTSQVTDELVQIIL
Sbjct: 121 KKQPWYGKPFIRSFQNLLRNTALGKYFFRAVATPESVKNILCQCYHDTSQVTDELVQIIL 180

Query: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESV 240
           NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNY SFESV
Sbjct: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYGSFESV 240

Query: 241 EDFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSA 277
           E+FVVLPNVGHCPQDEAPHLVNPLV+SFVS HATR+A
Sbjct: 241 EEFVVLPNVGHCPQDEAPHLVNPLVESFVSGHATRNA 277

BLAST of Moc02g00250 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.0e-20
Identity = 73/289 (25.26%), Postives = 123/289 (42.56%), Query Frame = 0

Query: 20  SDHWRKNISALAESHRVYALDLIG--------------------------YGYSDKPNPD 79
           S H+ K ++ L   +RV+A+D +G                          +G+ DK  P 
Sbjct: 129 SFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSSSEDKEPFWGFGDKTEP- 188

Query: 80  VVGESFYTFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNIS 139
              +  ++ + W  Q+  F  +V+ +  +   NS+GG V L  A   P + KG+ LLN +
Sbjct: 189 WADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFAATHPHLVKGVTLLNAT 248

Query: 140 ---------LRMLHIKKQ-PWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQC 199
                    +R   + +  PW G     +F    R   + +  ++ ++ PES+  IL Q 
Sbjct: 249 PFWGFFPNPVRSPKLARLFPWPG-----AFPLPERVKKITELVWQKISDPESIAEILKQV 308

Query: 200 YHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGGPLP-EELLPRVK---CPVLIAWG 259
           Y D S   D++   I+     P AA  F   +   GG L   E L R K     + + +G
Sbjct: 309 YTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCKENNVQICLMYG 368

Query: 260 DKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHLVNPLVKSFV 269
            +DPW     G+  +       +  +   GHCP DE P +VN L++ ++
Sbjct: 369 REDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYLMRGWI 411

BLAST of Moc02g00250 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.4e-15
Identity = 60/248 (24.19%), Postives = 107/248 (43.15%), Query Frame = 0

Query: 23  WRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEAF 82
           +RK I  L + + + ALDL  +G S+K    +     YT++  A  +      +   +A 
Sbjct: 43  FRKVIPLLRDKYDIIALDLPPFGQSEKSRTFI-----YTYQNLAKLVIGILEHLQVKQAV 102

Query: 83  FICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNTAL 142
            + +S+GG + L AA+ KP++   +VLL  S               L RS  +++  T +
Sbjct: 103 LVGHSMGGQISLSAALQKPELFSKVVLLCSS-------------GYLKRSHPTIIFGTHI 162

Query: 143 GKY--FFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSGG 202
             +  + +   + E V   L    HD S + +E++     P            FI +  G
Sbjct: 163 PYFHLYIKRWLSKEGVMKNLLNVVHDKSLIDEEMIDGYGRPFQDEQIFKAMTRFIRHREG 222

Query: 203 PLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHLV 262
            L  E L ++  P L+ WG++D   P+E+G+   +         L   GH   +E P L+
Sbjct: 223 DLEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELI 272

Query: 263 NPLVKSFV 269
           +  +  F+
Sbjct: 283 SEHIADFI 272

BLAST of Moc02g00250 vs. ExPASy Swiss-Prot
Match: Q9AQM4 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase OS=Pseudomonas resinovorans OX=53412 GN=carC PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.7e-10
Identity = 64/265 (24.15%), Postives = 118/265 (44.53%), Query Frame = 0

Query: 22  HWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD-E 81
           +WR  I  LA  +RV A+D++G+G + KP+ +      YT +     L+DF   +  D +
Sbjct: 54  NWRNVIPILARHYRVIAMDMLGFGKTAKPDIE------YTQDRRIRHLHDFIKAMNFDGK 113

Query: 82  AFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNT 141
              + NS+GG  GL  +V+  ++   +VL+  +  ++ I +     RP+I          
Sbjct: 114 VSIVGNSMGGATGLGVSVLHSELVNALVLMGSAGLVVEIHEDL---RPII---------- 173

Query: 142 ALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIF---LEFICY 201
               Y F    T E + +++    +D  ++ D ++               +   +++I  
Sbjct: 174 ---NYDF----TREGMVHLVKALTNDGFKIDDAMINSRYTYATDEATRKAYVATMQWIRE 233

Query: 202 SGGPLPE-ELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDF--VVLPNVGHCPQD 261
            GG   + E + +V  P L+  G  D   P+E    Y+  + ++D    ++P+ GH    
Sbjct: 234 QGGLFYDPEFIRKVPVPTLVVHGKDDKVVPVETA--YKFLDLIDDSWGYIIPHCGHWAMI 290

Query: 262 EAPHLVNPLVKSFVSRHA--TRSAS 278
           E P        SF+SR A  TR+A+
Sbjct: 294 EHPEDFANATLSFLSRRADITRAAA 290

BLAST of Moc02g00250 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 8.1e-10
Identity = 60/270 (22.22%), Postives = 108/270 (40.00%), Query Frame = 0

Query: 23  WRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEAF 82
           WR  +      +RV ALDL GYG SD P      +  Y  +   A + D    +   +  
Sbjct: 107 WRHQLREFKSEYRVVALDLRGYGESDAP----AHQESYKLDCLIADIKDILDSLGYSKCV 166

Query: 83  FICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRNTAL 142
            I +  GG++    AV  P++   ++++N     +       +   ++R    L R++  
Sbjct: 167 LIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSV-------FTEYILRHPAQLFRSSFY 226

Query: 143 GKYFFRTVATPE---SVKNILCQCYHDTSQVT--------------DELVQIILNPGLQP 202
             YFF+    PE   S+ +     +  TSQ T              +  V +   PG   
Sbjct: 227 --YFFQIPRFPEFMFSINDFKALKHLFTSQSTGIGRKGRQLTTEDLEAYVYVFSQPGALS 286

Query: 203 GAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSF-ESVEDFVV 262
           G  + +     +S  PL   +   V  P L+ WG++D +  +E+    + + ++     +
Sbjct: 287 GPINHYRNI--FSCLPLKHHM---VTTPTLLLWGEEDAFMEVEMAEVTKIYVKNYFRLTI 346

Query: 263 LPNVGHCPQDEAPHLVNPLVKSFVSRHATR 275
           L    H  Q + P +VN L+ +F+     R
Sbjct: 347 LSEGSHWLQQDQPDIVNGLIWAFLKEETRR 358

BLAST of Moc02g00250 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.8e-09
Identity = 53/266 (19.92%), Postives = 108/266 (40.60%), Query Frame = 0

Query: 23  WRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEAF 82
           WR  +      +RV ALDL GYG +D P    +    Y  +     + D    +   +  
Sbjct: 109 WRYQLREFKSEYRVVALDLRGYGETDAP----IHRQNYKLDCLITDIKDILDSLGYSKCV 168

Query: 83  FICNSIGGVVGLQAAVMKPQICKGIVLLNI---SLRMLHIKKQP----------WYGRPL 142
            I +  GG++    A+  P++   ++++N    ++   +I + P          ++  P 
Sbjct: 169 LIGHDWGGMIAWLIAICYPEMVMKLIVINFPHPNVFTEYILRHPAQLLKSSYYYFFQIPW 228

Query: 143 IRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD 202
              F   + +  + K+ F + +T    K   CQ    T++  +  + +   PG   G  +
Sbjct: 229 FPEFMFSINDFKVLKHLFTSHSTGIGRKG--CQL---TTEDLEAYIYVFSQPGALSGPIN 288

Query: 203 IFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSF-ESVEDFVVLPNV 262
            +     +S  PL   +   V  P L+ WG+ D +  +E+    + + ++     +L   
Sbjct: 289 HYRNI--FSCLPLKHHM---VTTPTLLLWGENDAFMEVEMAEVTKIYVKNYFRLTILSEA 348

Query: 263 GHCPQDEAPHLVNPLVKSFVSRHATR 275
            H  Q + P +VN L+ +F+     +
Sbjct: 349 SHWLQQDQPDIVNKLIWTFLKEETRK 360

BLAST of Moc02g00250 vs. ExPASy TrEMBL
Match: A0A6J1DI14 (pheophytinase, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111021274 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.2e-165
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MVLEQTGLHSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFY 60
           MVLEQTGLHSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFY
Sbjct: 1   MVLEQTGLHSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFY 60

Query: 61  TFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIK 120
           TFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIK
Sbjct: 61  TFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIK 120

Query: 121 KQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILN 180
           KQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILN
Sbjct: 121 KQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILN 180

Query: 181 PGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVE 240
           PGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVE
Sbjct: 181 PGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVE 240

Query: 241 DFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSASACTSSG 284
           DFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSASACTSSG
Sbjct: 241 DFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSASACTSSG 283

BLAST of Moc02g00250 vs. ExPASy TrEMBL
Match: A0A6J1DIP0 (pheophytinase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021274 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.6e-154
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 20  SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD 79
           SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD
Sbjct: 115 SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD 174

Query: 80  EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 139
           EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN
Sbjct: 175 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 234

Query: 140 TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 199
           TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG
Sbjct: 235 TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 294

Query: 200 GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL 259
           GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL
Sbjct: 295 GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL 354

Query: 260 VNPLVKSFVSRHATRSASACTSSG 284
           VNPLVKSFVSRHATRSASACTSSG
Sbjct: 355 VNPLVKSFVSRHATRSASACTSSG 378

BLAST of Moc02g00250 vs. ExPASy TrEMBL
Match: A0A1S3BN16 (pheophytinase, chloroplastic isoform X4 OS=Cucumis melo OX=3656 GN=LOC103491627 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 6.0e-149
Identity = 253/277 (91.34%), Postives = 265/277 (95.67%), Query Frame = 0

Query: 1   MVLEQTGL-HSNFASDTTKYSDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESF 60
           MVLEQTGL HS FAS TT YSDHWRKNI  LA+SHRVYA+DLIGYGYSDKPNP++VGE F
Sbjct: 1   MVLEQTGLHHSIFASHTTNYSDHWRKNIPVLAQSHRVYAIDLIGYGYSDKPNPELVGEGF 60

Query: 61  YTFETWAAQLNDFCVDVVQDEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHI 120
           YTFETWA+QLNDFCVDVVQD+AFFICNSIGGVVGLQAA+MKPQIC GIVLLNISLRMLHI
Sbjct: 61  YTFETWASQLNDFCVDVVQDDAFFICNSIGGVVGLQAAIMKPQICNGIVLLNISLRMLHI 120

Query: 121 KKQPWYGRPLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIIL 180
           KKQPWYG+P IRSFQ+LLRNTALGKYFFR VATPESVKNILCQCYHDTSQVTDELVQIIL
Sbjct: 121 KKQPWYGKPFIRSFQNLLRNTALGKYFFRAVATPESVKNILCQCYHDTSQVTDELVQIIL 180

Query: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESV 240
           NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNY SFESV
Sbjct: 181 NPGLQPGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYGSFESV 240

Query: 241 EDFVVLPNVGHCPQDEAPHLVNPLVKSFVSRHATRSA 277
           E+FVVLPNVGHCPQDEAPHLVNPLV+SFVS HATR+A
Sbjct: 241 EEFVVLPNVGHCPQDEAPHLVNPLVESFVSGHATRNA 277

BLAST of Moc02g00250 vs. ExPASy TrEMBL
Match: A0A6J1H3U7 (pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111459754 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.6e-144
Identity = 241/257 (93.77%), Postives = 252/257 (98.05%), Query Frame = 0

Query: 20  SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD 79
           SDHWRKNI ALAESHRVYA+DLIGYGYSDKPNPD+VGESFYTFETWA+QLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 80  EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 139
           EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 140 TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 199
           TALGKYFF TVATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFTTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 200 GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL 259
           GPLPEELLP VKCPVLI WGDKDPWEPIELGRNY +FESVEDFVV+PNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYENFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 260 VNPLVKSFVSRHATRSA 277
           VNPLV+SFV+RHATRSA
Sbjct: 361 VNPLVESFVTRHATRSA 377

BLAST of Moc02g00250 vs. ExPASy TrEMBL
Match: A0A6J1K1N6 (pheophytinase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111490908 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.9e-144
Identity = 240/257 (93.39%), Postives = 253/257 (98.44%), Query Frame = 0

Query: 20  SDHWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQD 79
           SDHWRKNI ALAESHRVYA+DLIGYGYSDKPNPD+VGESFYTFETWA+QLNDFCVDVVQD
Sbjct: 121 SDHWRKNIPALAESHRVYAVDLIGYGYSDKPNPDLVGESFYTFETWASQLNDFCVDVVQD 180

Query: 80  EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLRN 139
           EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYG+PLI+SFQSLLRN
Sbjct: 181 EAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGKPLIKSFQSLLRN 240

Query: 140 TALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYSG 199
           TALGKYFF+TVATPE+VKNILCQCYHDTSQVTDELVQIILNPGLQPGAAD+FLEFICYSG
Sbjct: 241 TALGKYFFKTVATPEAVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADVFLEFICYSG 300

Query: 200 GPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPHL 259
           GPLPEELLP VKCPVLI WGDKDPWEPIELGRNY++FESVEDFVV+PNVGHCPQDEAPHL
Sbjct: 301 GPLPEELLPNVKCPVLIGWGDKDPWEPIELGRNYKNFESVEDFVVIPNVGHCPQDEAPHL 360

Query: 260 VNPLVKSFVSRHATRSA 277
           VNPLV+SFV+RHAT SA
Sbjct: 361 VNPLVESFVTRHATSSA 377

BLAST of Moc02g00250 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 432.2 bits (1110), Expect = 4.0e-121
Identity = 192/253 (75.89%), Postives = 228/253 (90.12%), Query Frame = 0

Query: 20  SDHWRKNISALAESHRVYALDLIGYGYSDKPNP-DVVGESFYTFETWAAQLNDFCVDVVQ 79
           SDHWRKN   L ++HRVY++DLIGYGYSDKPNP +  GE FYTFETW  QLNDFC+DVV+
Sbjct: 107 SDHWRKNTPILGKTHRVYSIDLIGYGYSDKPNPREFGGEPFYTFETWGEQLNDFCLDVVK 166

Query: 80  DEAFFICNSIGGVVGLQAAVMKPQICKGIVLLNISLRMLHIKKQPWYGRPLIRSFQSLLR 139
           DEAFFICNSIGG+VGLQAAV KP+IC+G++L+NISLRMLHIKKQP+ GRP I+SFQ+LLR
Sbjct: 167 DEAFFICNSIGGLVGLQAAVSKPEICRGLMLINISLRMLHIKKQPFIGRPFIKSFQNLLR 226

Query: 140 NTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFICYS 199
           NT +GK FF+++A PE+VK+ILCQCYHD+SQVTDELV+ IL PGL+PGA D+FLEFICYS
Sbjct: 227 NTPVGKLFFKSIAKPETVKSILCQCYHDSSQVTDELVEAILRPGLEPGAVDVFLEFICYS 286

Query: 200 GGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHCPQDEAPH 259
           GGPLPE+LLP VKCPVLIAWG+KDPWEPIELGR Y +F++VEDFVVLP+ GHCPQDE P 
Sbjct: 287 GGPLPEDLLPLVKCPVLIAWGEKDPWEPIELGRAYSNFDAVEDFVVLPDAGHCPQDEKPE 346

Query: 260 LVNPLVKSFVSRH 272
           +VNPL++SFV+RH
Sbjct: 347 MVNPLIESFVARH 359

BLAST of Moc02g00250 vs. TAIR 10
Match: AT5G38520.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 4.2e-38
Identity = 95/251 (37.85%), Postives = 137/251 (54.58%), Query Frame = 0

Query: 22  HWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEA 81
           HWR+NI+AL+++H VYA+DL+G+G SDKP     G S YT E+WA  + +F  +VVQ   
Sbjct: 105 HWRRNINALSKNHTVYAIDLLGFGASDKP----PGFS-YTMESWAELILNFLEEVVQKPT 164

Query: 82  FFICNSIGGVV-GLQAAVMKPQICKGIVLLNISLRMLH-IKKQPWYGR---PLIRSFQSL 141
             I NS+G +   + A+  +  + KG+VLLN +  M +      W  +   PL+     L
Sbjct: 165 ILIGNSVGSLACVIAASESRGDLVKGLVLLNCAGGMNNKAVFDDWRIKLLMPLLLLIDFL 224

Query: 142 LRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIFLEFIC 201
           L+   +    F  V   E++KNIL   Y +   V D LV+II  P    GA D F+  + 
Sbjct: 225 LKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIAGPANTEGALDAFVSILT 284

Query: 202 YSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVED------FVVLPNVGH 261
              GP P +L+P +  PVL+ WGD+D   P++ G   + F S+ D        VL  VGH
Sbjct: 285 GPPGPNPIKLIPEITKPVLVLWGDQDGLTPLD-GPVGKYFTSLPDQLPNFNLYVLQGVGH 344

BLAST of Moc02g00250 vs. TAIR 10
Match: AT5G38520.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 1.0e-36
Identity = 96/263 (36.50%), Postives = 137/263 (52.09%), Query Frame = 0

Query: 22  HWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEA 81
           HWR+NI+AL+++H VYA+DL+G+G SDKP     G S YT E+WA  + +F  +VVQ   
Sbjct: 105 HWRRNINALSKNHTVYAIDLLGFGASDKP----PGFS-YTMESWAELILNFLEEVVQKPT 164

Query: 82  FFICNSIGGVVGLQAA-------------VMKPQICKGIVLLNISLRMLH-IKKQPWYGR 141
             I NS+G +  + AA               +  + KG+VLLN +  M +      W  +
Sbjct: 165 ILIGNSVGSLACVIAASGTKFLIYLEKKTESRGDLVKGLVLLNCAGGMNNKAVFDDWRIK 224

Query: 142 ---PLIRSFQSLLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQ 201
              PL+     LL+   +    F  V   E++KNIL   Y +   V D LV+II  P   
Sbjct: 225 LLMPLLLLIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLVEIIAGPANT 284

Query: 202 PGAADIFLEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVED--- 261
            GA D F+  +    GP P +L+P +  PVL+ WGD+D   P++ G   + F S+ D   
Sbjct: 285 EGALDAFVSILTGPPGPNPIKLIPEITKPVLVLWGDQDGLTPLD-GPVGKYFTSLPDQLP 344

BLAST of Moc02g00250 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 139.0 bits (349), Expect = 7.0e-33
Identity = 79/258 (30.62%), Postives = 125/258 (48.45%), Query Frame = 0

Query: 22  HWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEA 81
           HWR NI  LA+ ++VYALDL+G+G+SDK   +      Y    W  Q+ DF  +VV++ A
Sbjct: 57  HWRYNIPELAKKYKVYALDLLGFGWSDKALIE------YDAMVWTDQVIDFMKEVVKEPA 116

Query: 82  FFICNSIGGVVGLQAAVMKPQICKGIVLLN------ISLRMLHIKKQPWYGRPLIRSFQS 141
             + NS+GG   L  AV  P+   G+ LLN         R      +    + +++  + 
Sbjct: 117 VVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKE 176

Query: 142 LLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIF---- 201
           + +   LG + F     P  ++++L   Y D++ V D LV+ I  P   P A +++    
Sbjct: 177 IFQRVVLG-FLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLM 236

Query: 202 LEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHC 261
             F+        + +L ++ CP+L+ WGD DPW         ++F S    V L   GHC
Sbjct: 237 TRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHL-QAGHC 296

Query: 262 PQDEAPHLVNPLVKSFVS 270
           P DE P  VN  +  ++S
Sbjct: 297 PHDEVPEAVNKALLDWLS 306

BLAST of Moc02g00250 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 139.0 bits (349), Expect = 7.0e-33
Identity = 79/258 (30.62%), Postives = 125/258 (48.45%), Query Frame = 0

Query: 22  HWRKNISALAESHRVYALDLIGYGYSDKPNPDVVGESFYTFETWAAQLNDFCVDVVQDEA 81
           HWR NI  LA+ ++VYALDL+G+G+SDK   +      Y    W  Q+ DF  +VV++ A
Sbjct: 114 HWRYNIPELAKKYKVYALDLLGFGWSDKALIE------YDAMVWTDQVIDFMKEVVKEPA 173

Query: 82  FFICNSIGGVVGLQAAVMKPQICKGIVLLN------ISLRMLHIKKQPWYGRPLIRSFQS 141
             + NS+GG   L  AV  P+   G+ LLN         R      +    + +++  + 
Sbjct: 174 VVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKE 233

Query: 142 LLRNTALGKYFFRTVATPESVKNILCQCYHDTSQVTDELVQIILNPGLQPGAADIF---- 201
           + +   LG + F     P  ++++L   Y D++ V D LV+ I  P   P A +++    
Sbjct: 234 IFQRVVLG-FLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLM 293

Query: 202 LEFICYSGGPLPEELLPRVKCPVLIAWGDKDPWEPIELGRNYRSFESVEDFVVLPNVGHC 261
             F+        + +L ++ CP+L+ WGD DPW         ++F S    V L   GHC
Sbjct: 294 TRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHL-QAGHC 353

Query: 262 PQDEAPHLVNPLVKSFVS 270
           P DE P  VN  +  ++S
Sbjct: 354 PHDEVPEAVNKALLDWLS 363