Moc02g00060 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g00060
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionL-ascorbate oxidase homolog
Locationchr2: 25206 .. 27044 (-)
RNA-Seq ExpressionMoc02g00060
SyntenyMoc02g00060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGGGTTGATGTTTACCGTGCTGCTATGCCTCTCGGCTGCAGCAATGTCAACGGTCCGGGGTGAGGACCCTTATTTCTTCTTCACGTGGAATGTCACCTATGGCACCATCACTCCCTTGGGCGTTACCCAACAAGGCATTCTCATTAACGGCCAATTCCCCGGACCCAATATCAACTCCACATCCAACAATAACATAGTCATTAATGTCTTCAACAACCTCGACGAACCCTTCCTTCTACATTGGTACAGTAATTATCTTTATATTTACATATGCACGCATATATAACTCTTTCTTCTTCATTCAATGGCTCAGTACAAAAAAATAAAATCAAATGGAATTGGACTTGCAGGAGCGGTATTCAACACCGCAAAAATTCGTGGCAAGATGGACTACTTGGAACCAATTGCCCAATCCCACCGGGGACAAACTACACCTACCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTTTACTATCCATCAACTGCCATGCACAGGGCAGCGGGAGGCTTTGGAGGCCTTCGTGTGAATAGCCGTTTACTCATTCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTCCTTGCTGGTGACTGGTACACCAAGAGCCACTCCTCTCTTAAGAATTTCTTGGATAGCGGACGCTCCCTCGCTAGGCCTGACGGTGTCCTCATTAATGGGAAGAATGCAAAGGGTGATGGAACCGATGAGCCGCTCTTTACCATGAAGCCTGAGAAGACTTACAAGTACCGAGTTTGCAACATTGGACTGAAGACATCTCTTAACATTAGATTCCAAGGCCACACCATGAAGTTGGTAGAGATGGAGGGCTCTCATGTCGTCCAGAACGATTACCAATCACTTGACATCCATGTGGGACAATGCTTTTCGGTGCTAGTCACAGCCAACCAAGAGCCCAAGGACTATTACTTGATTGCATCCACTCGATTTACTAAGAATGTTCTTGTCGGCAAAGGCATCATTCGATATACCAATGGCAAAGGCCCCGCCTCGCCTGAAATCCCCGAGGCACCTACGGGTTGGGCTTGGTCGCTCAATCAATTCCGTACCTTCCGTTGGAACCTTACCGCTAGTGCTGCTAGGCCTAATCCCCAAGGCTCGTACCACTACGGTTCCATCAATATTACCCGCACGATCAAGATAATCAATTCAGCCACTAAGGTGGATGGTAAGCTGCGATATGCCATCAATGGTGTCTCTCATATTGACCCTGAAACTCCATTGAAGCTCGCAGAGTACTTTGGAATCGTTGACAAGGTCTTCAAGTACGATACCATTTCTGACGACGGACGAGCAGAAGGTGCAAACACCGTGACTGTTGCTCCTAATGTTGTTAATGCAACTTTCCGAAACTTCGTAGAGATCGTATTTGAGAACCATGAGAAAAGCCTTCAATCGTGGCATTTGAATGGCTACTCCTTCTTCGCTGTTGCGTAAGTATCATCAATTAGAAATAATCAATAATGTTTATATATGACTAATTTACTCTTTATCAACATTACACAGCATTGAACCTGGGAAATGGGCTCCAGAAAAACGAAAGAACTACAATCTTTTGGACGCTGTGAGCAGGCATACAATCCAAGTTTTTCCCAAGTCGTGGGCAGCCGTTCTTCTTACATTAGATAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGACAGAAAATCGTTATTTGGGACAACAACTCTACTTCAGCGTTCTTTCTCCAGCTCGCTCACTCAGAGATGAATACAACATTCCAGACAACACTTTACTATGTGGTTTGGTTAAAGACATGGCATTGCCCAAGCCATATACTATTTGA

mRNA sequence

ATGGGTGGGTTGATGTTTACCGTGCTGCTATGCCTCTCGGCTGCAGCAATGTCAACGGTCCGGGGTGAGGACCCTTATTTCTTCTTCACGTGGAATGTCACCTATGGCACCATCACTCCCTTGGGCGTTACCCAACAAGGCATTCTCATTAACGGCCAATTCCCCGGACCCAATATCAACTCCACATCCAACAATAACATAGTCATTAATGTCTTCAACAACCTCGACGAACCCTTCCTTCTACATTGGAGCGGTATTCAACACCGCAAAAATTCGTGGCAAGATGGACTACTTGGAACCAATTGCCCAATCCCACCGGGGACAAACTACACCTACCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTTTACTATCCATCAACTGCCATGCACAGGGCAGCGGGAGGCTTTGGAGGCCTTCGTGTGAATAGCCGTTTACTCATTCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTCCTTGCTGGTGACTGGTACACCAAGAGCCACTCCTCTCTTAAGAATTTCTTGGATAGCGGACGCTCCCTCGCTAGGCCTGACGGTGTCCTCATTAATGGGAAGAATGCAAAGGGTGATGGAACCGATGAGCCGCTCTTTACCATGAAGCCTGAGAAGACTTACAAGTACCGAGTTTGCAACATTGGACTGAAGACATCTCTTAACATTAGATTCCAAGGCCACACCATGAAGTTGGTAGAGATGGAGGGCTCTCATGTCGTCCAGAACGATTACCAATCACTTGACATCCATGTGGGACAATGCTTTTCGGTGCTAGTCACAGCCAACCAAGAGCCCAAGGACTATTACTTGATTGCATCCACTCGATTTACTAAGAATGTTCTTGTCGGCAAAGGCATCATTCGATATACCAATGGCAAAGGCCCCGCCTCGCCTGAAATCCCCGAGGCACCTACGGGTTGGGCTTGGTCGCTCAATCAATTCCGTACCTTCCGTTGGAACCTTACCGCTAGTGCTGCTAGGCCTAATCCCCAAGGCTCGTACCACTACGGTTCCATCAATATTACCCGCACGATCAAGATAATCAATTCAGCCACTAAGGTGGATGGTAAGCTGCGATATGCCATCAATGGTGTCTCTCATATTGACCCTGAAACTCCATTGAAGCTCGCAGAGTACTTTGGAATCGTTGACAAGGTCTTCAAGTACGATACCATTTCTGACGACGGACGAGCAGAAGGTGCAAACACCGTGACTGTTGCTCCTAATGTTGTTAATGCAACTTTCCGAAACTTCGTAGAGATCGTATTTGAGAACCATGAGAAAAGCCTTCAATCGTGGCATTTGAATGGCTACTCCTTCTTCGCTGTTGCCATTGAACCTGGGAAATGGGCTCCAGAAAAACGAAAGAACTACAATCTTTTGGACGCTGTGAGCAGGCATACAATCCAAGTTTTTCCCAAGTCGTGGGCAGCCGTTCTTCTTACATTAGATAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGACAGAAAATCGTTATTTGGGACAACAACTCTACTTCAGCGTTCTTTCTCCAGCTCGCTCACTCAGAGATGAATACAACATTCCAGACAACACTTTACTATGTGGTTTGGTTAAAGACATGGCATTGCCCAAGCCATATACTATTTGA

Coding sequence (CDS)

ATGGGTGGGTTGATGTTTACCGTGCTGCTATGCCTCTCGGCTGCAGCAATGTCAACGGTCCGGGGTGAGGACCCTTATTTCTTCTTCACGTGGAATGTCACCTATGGCACCATCACTCCCTTGGGCGTTACCCAACAAGGCATTCTCATTAACGGCCAATTCCCCGGACCCAATATCAACTCCACATCCAACAATAACATAGTCATTAATGTCTTCAACAACCTCGACGAACCCTTCCTTCTACATTGGAGCGGTATTCAACACCGCAAAAATTCGTGGCAAGATGGACTACTTGGAACCAATTGCCCAATCCCACCGGGGACAAACTACACCTACCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTTTACTATCCATCAACTGCCATGCACAGGGCAGCGGGAGGCTTTGGAGGCCTTCGTGTGAATAGCCGTTTACTCATTCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTCCTTGCTGGTGACTGGTACACCAAGAGCCACTCCTCTCTTAAGAATTTCTTGGATAGCGGACGCTCCCTCGCTAGGCCTGACGGTGTCCTCATTAATGGGAAGAATGCAAAGGGTGATGGAACCGATGAGCCGCTCTTTACCATGAAGCCTGAGAAGACTTACAAGTACCGAGTTTGCAACATTGGACTGAAGACATCTCTTAACATTAGATTCCAAGGCCACACCATGAAGTTGGTAGAGATGGAGGGCTCTCATGTCGTCCAGAACGATTACCAATCACTTGACATCCATGTGGGACAATGCTTTTCGGTGCTAGTCACAGCCAACCAAGAGCCCAAGGACTATTACTTGATTGCATCCACTCGATTTACTAAGAATGTTCTTGTCGGCAAAGGCATCATTCGATATACCAATGGCAAAGGCCCCGCCTCGCCTGAAATCCCCGAGGCACCTACGGGTTGGGCTTGGTCGCTCAATCAATTCCGTACCTTCCGTTGGAACCTTACCGCTAGTGCTGCTAGGCCTAATCCCCAAGGCTCGTACCACTACGGTTCCATCAATATTACCCGCACGATCAAGATAATCAATTCAGCCACTAAGGTGGATGGTAAGCTGCGATATGCCATCAATGGTGTCTCTCATATTGACCCTGAAACTCCATTGAAGCTCGCAGAGTACTTTGGAATCGTTGACAAGGTCTTCAAGTACGATACCATTTCTGACGACGGACGAGCAGAAGGTGCAAACACCGTGACTGTTGCTCCTAATGTTGTTAATGCAACTTTCCGAAACTTCGTAGAGATCGTATTTGAGAACCATGAGAAAAGCCTTCAATCGTGGCATTTGAATGGCTACTCCTTCTTCGCTGTTGCCATTGAACCTGGGAAATGGGCTCCAGAAAAACGAAAGAACTACAATCTTTTGGACGCTGTGAGCAGGCATACAATCCAAGTTTTTCCCAAGTCGTGGGCAGCCGTTCTTCTTACATTAGATAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGACAGAAAATCGTTATTTGGGACAACAACTCTACTTCAGCGTTCTTTCTCCAGCTCGCTCACTCAGAGATGAATACAACATTCCAGACAACACTTTACTATGTGGTTTGGTTAAAGACATGGCATTGCCCAAGCCATATACTATTTGA

Protein sequence

MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMALPKPYTI
Homology
BLAST of Moc02g00060 vs. NCBI nr
Match: XP_022153757.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 544/552 (98.55%), Postives = 545/552 (98.73%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           MGGL+FTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTI PLGV QQGILINGQFPGPNIN
Sbjct: 1   MGGLIFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTIXPLGVXQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHS LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAXGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSXLKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPXGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIXDKVFKYDTISDDGXAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMALPKPYTI 553
           VKDMALPKPYTI
Sbjct: 541 VKDMALPKPYTI 552

BLAST of Moc02g00060 vs. NCBI nr
Match: XP_022153749.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 535/552 (96.92%), Postives = 542/552 (98.19%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           M GLMFTVLLCLSAAAMSTVRGEDPYFFFTWN+TYGTI+PLGV QQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLD 180
           GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSHS+LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQ HTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPG+W PEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAA+LLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMALPKPYTI 553
           VKDM LPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of Moc02g00060 vs. NCBI nr
Match: KAA0040123.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 501/553 (90.60%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAA-MSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M GLMFT LLCLSAAA M+TV+GEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH++LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGI DKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMALPKPYTI 553
           LVKDM +PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Moc02g00060 vs. NCBI nr
Match: KAA0040126.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1074.3 bits (2777), Expect = 4.2e-310
Identity = 501/553 (90.60%), Postives = 530/553 (95.84%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAA-MSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M  LMFT LLCLSAAA M+TVRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH++LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGI DKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMALPKPYTI 553
           LVKDM +PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Moc02g00060 vs. NCBI nr
Match: KAA0040130.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa] >TYK21751.1 L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1072.8 bits (2773), Expect = 8.5e-310
Identity = 498/553 (90.05%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMST-VRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M GLMFT LLCLSAAAM+T VRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MAGLMFTTLLCLSAAAMTTMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NST+NNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSH++LK FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLKTSLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDY+SLD+HVGQCFSVLVTA+QEPKDYY++ASTRF K+ LVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYKSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKSALVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           NGKGPASPE+P AP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 NGKGPASPELPNAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFG+ DKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGVTDKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VN TFRNF+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W PEKR +YNLLDAVSRHTIQ
Sbjct: 421 VNTTFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRSDYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSEL ENRYLGQQLY SV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELAENRYLGQQLYMSVQSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMALPKPYTI 553
           LVKD+ LPKPYTI
Sbjct: 541 LVKDLPLPKPYTI 553

BLAST of Moc02g00060 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 2.2e-259
Identity = 406/548 (74.09%), Postives = 464/548 (84.67%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           G  +    L L+AAA   V  EDPYF   WNVTYGT +PLGV QQ ILINGQFPGPNINS
Sbjct: 3   GVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 62

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTNYTYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 122 SFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDS 181
           S+FYYP+T MHRAAGG+GGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK FLD 
Sbjct: 123 SYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDG 182

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR++ RPDG++INGK+ KGDG+D PLFT+KP KTY+ R+CN+G+KTS+N R Q H MKLV
Sbjct: 183 GRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLV 242

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQCF  +VTANQEPKDYY++AS+RF K V+   G++RY  G
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGG 302

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
           KGPAS ++P  P GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  
Sbjct: 303 KGPASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 362

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVN 421
           KVDGKLR+A+NGVSH +PETPLKLAEYFGI DKVFKYDTI+DD   E    + + PNV+N
Sbjct: 363 KVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLN 422

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            T R FVE+VFENHEKS+QSWHL+GYSFF+VA+EPG W PEKRKNYNLLDAVSRHT+QV+
Sbjct: 423 ITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 482

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PK WAA+LLT DN GMWN+RSE TE RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLV
Sbjct: 483 PKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 542

Query: 542 KDMALPKP 550
           K+   PKP
Sbjct: 543 KN--TPKP 548

BLAST of Moc02g00060 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 8.1e-238
Identity = 375/547 (68.56%), Postives = 450/547 (82.27%), Query Frame = 0

Query: 8   VLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSNNNI 67
           +LLCLS   ++    EDPY +F WNVTYGTI PLGV QQGILINGQFPGP IN TSNNNI
Sbjct: 11  LLLCLSVGVIA----EDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNI 70

Query: 68  VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYP 127
           V+NVFNNLDEPFL  W+G+QHRKNSWQDG  GT CPI PG N+TY FQVKDQIGS+ Y+P
Sbjct: 71  VVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFP 130

Query: 128 STAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDSGRSLAR 187
           +TA+HRAAGG+G L V+SR LIPVP+ +P D+Y V  GDWY K H +LK  LD GR++ R
Sbjct: 131 TTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGR 190

Query: 188 PDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEMEGSH 247
           PDG++INGK+AK     EPLFTM+  KTY+YR CN+G+++S+NIRFQGH MKLVE+EGSH
Sbjct: 191 PDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSH 250

Query: 248 VVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGPASP 307
            VQN Y SLD+HVGQC SVLVTA+QEPKDYYL+ S+RF K  L    IIRY NGKGPASP
Sbjct: 251 TVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASP 310

Query: 308 EIPEAP----TGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKV 367
           E+P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIKI NS ++V
Sbjct: 311 ELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQV 370

Query: 368 DGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVNAT 427
            GKLRY +NG+SH + ETPLKL EYFG  +K FKYD ++D+  A+  + +T+A NV NAT
Sbjct: 371 GGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPAD-PSKLTIATNVKNAT 430

Query: 428 FRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPK 487
           +RNFVEI+FENHEK+++++HL+GYSFFAVA+EPG+W+PEKRKNYNL+D +SR+ IQV+P 
Sbjct: 431 YRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPN 490

Query: 488 SWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKD 547
           SWAA++LT DNAGMWNLRSE+ E  YLG+QLYFSVLSP+RSLRDEYNIPDN  LCG+VK 
Sbjct: 491 SWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 550

Query: 548 MALPKPY 551
           +++P PY
Sbjct: 551 LSMPAPY 552

BLAST of Moc02g00060 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 2.9e-155
Identity = 265/560 (47.32%), Postives = 364/560 (65.00%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSN 64
           +F +LL +    +S     DPY F+ + V+Y T +PLGV QQ I ING+FPGP IN T+N
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
            N+V+NV N LDE  LLHW+GIQ R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFF
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDSGRS 184
           Y+PS    RA+GGFG   VN R +IPVP++ P+ D TV  GDWY ++H++L+  LD G+ 
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 185 LARPDGVLINGK-------NAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHT 244
           L  PDGVLINGK           DG D    T+ P KTY+ RV N+G+ TSLN R QGH 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 245 MKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQE-PKDYYLIASTRFTKNVL----VG 304
           + L E EGS+ VQ +Y SLDIHVGQ +S LVT +Q    DYY++AS R     +     G
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 305 KGIIRYTNGKGPASPEIPEAPT---GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSIN 364
            GI++YTN KG A  ++P  P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 365 ITRTIKIIN-SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAE 424
           +T    + N     + GK R  +NG+S  +P TP++LA+   + D V+K D      +  
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLDF----PKRP 422

Query: 425 GANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYN 484
                 VA +++N T+R F+E+V +N++  +QS+H++GY+FF V ++ G+W    R  YN
Sbjct: 423 LTGPAKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYN 482

Query: 485 LLDAVSRHTIQVFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDE 544
             D ++R TIQV+P +W+A+L++LDN G WNLR+E  ++ YLGQ+ Y  V++P  + + E
Sbjct: 483 KWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTE 542

Query: 545 YNIPDNTLLCGLVKDMALPK 549
           +  PDN L CG +  +  P+
Sbjct: 543 FGHPDNVLYCGALSKLQKPQ 557

BLAST of Moc02g00060 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.3e-147
Identity = 246/538 (45.72%), Postives = 363/538 (67.47%), Query Frame = 0

Query: 24  DPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSNNNIVINVFNNLDEPFLLHW 83
           DPY  + + ++Y T +PLGV QQ I +NG+FPGP IN+T+N N+ +NV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 84  SGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRV 143
            G+Q R+NSWQDG+LGTNCPIPP  N+TY FQ+KDQIGS+FY PS    RA+GGFG L +
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 144 NSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDSGRSLARPDGVLINGK------N 203
           N+R L+P+P+ +P+ +   + GDWYT++H++L+  LDSG+ L  PDGVLINGK      +
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 204 AKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEMEGSHVVQNDYQSLD 263
           +  DG +     + P KTY+ RV N+G+ TSLN R Q H + L+E EG +  Q ++   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 264 IHVGQCFSVLVTANQE-PKDYYLIASTRFTKNV----LVGKGIIRYTNGKGPASPEIPEA 323
           +HVGQ +S LVT +Q    DYY++AS RF        + G GI+ Y+N KGPAS  +P +
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 324 PT--GWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS--ATKVDGKL 383
            T     WS +NQ R  + N +AS ARPNPQGS+HYG INITRT  I+ S   TK++GKL
Sbjct: 325 ATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTY-ILRSLPPTKINGKL 384

Query: 384 RYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISD-DGRAEGANTVTVAPNVVNATFRN 443
           R  +NG+S ++P TP++LA+     D   K D + D   R        ++ +++NAT++ 
Sbjct: 385 RATLNGISFVNPSTPMRLAD-----DHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKG 444

Query: 444 FVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPKSWA 503
           F++++F+N++  +QS+H++GY+F+ VA++ G W+ ++  +YN  DAV+R T++V+P +W 
Sbjct: 445 FIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWT 504

Query: 504 AVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 545
           AVL++LDN G+WN+R E  +  YLGQ+ Y  +++P  +   E + P+N + CG ++ M
Sbjct: 505 AVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of Moc02g00060 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.5e-146
Identity = 258/558 (46.24%), Postives = 369/558 (66.13%), Query Frame = 0

Query: 4   LMFTVLLCLSAAAMSTVR-GEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINST 63
           L+ + LLC   A +S V    DP+  + + V+Y T +PLGV QQ I +NGQFPGP +N+T
Sbjct: 7   LLASFLLCF--ALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNAT 66

Query: 64  SNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGS 123
           +N N+V+NVFN+LDEP LL W GIQ R+NSWQDG+LGTNCPIPP  N+TY FQVKDQIGS
Sbjct: 67  TNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGS 126

Query: 124 FFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDSG 183
           FFY PS    RA+GGFG + +N+R +IP+P+  P+ +   + GDWYT+ H +L+  LDSG
Sbjct: 127 FFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSG 186

Query: 184 RSLARPDGVLINGK------NAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGH 243
           + L  PDGVLINGK      ++  DG D   F ++P KTY+ RV N+G+ TSLN R Q H
Sbjct: 187 KELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNH 246

Query: 244 TMKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQE-PKDYYLIASTRFTKNV----LV 303
           ++ LVE EG +  Q ++   D+HVGQ +S LVT +Q+   DYY++AS RF        + 
Sbjct: 247 SLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVT 306

Query: 304 GKGIIRYTNGKGPASPEIPEAPTGWA--WS-LNQFRTFRWNLTASAARPNPQGSYHYGSI 363
           G  I+ Y+N KGP S  +P   T  +  WS ++Q +T R N +AS ARPNPQGS+HYG I
Sbjct: 307 GVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQI 366

Query: 364 NITRTIKIINS--ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGR 423
           NIT T  I+ S   T ++G LR  +NG+S ++P TP++LA+    V   +K D       
Sbjct: 367 NITNTY-ILRSLPPTIINGALRATLNGISFVNPSTPVRLADR-NKVKGAYKLDFPDRPFN 426

Query: 424 AEGANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKN 483
                 + +  +++NAT++ F+++VF+N++  +QS+H++GYSFF V ++ G W+ +K+ +
Sbjct: 427 ----RPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGS 486

Query: 484 YNLLDAVSRHTIQVFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLR 543
           YN  DA+SR TI+V+P  W AVL++LDN G+WN+R E  +  YLG++ Y  + +P    +
Sbjct: 487 YNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGK 546

Query: 544 DEYNIPDNTLLCGLVKDM 545
            E + PDN L CG +K++
Sbjct: 547 TEMDPPDNVLYCGALKNL 556

BLAST of Moc02g00060 vs. ExPASy TrEMBL
Match: A0A6J1DJT4 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021199 PE=3 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 544/552 (98.55%), Postives = 545/552 (98.73%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           MGGL+FTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTI PLGV QQGILINGQFPGPNIN
Sbjct: 1   MGGLIFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTIXPLGVXQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHS LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAXGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSXLKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPXGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIXDKVFKYDTISDDGXAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMALPKPYTI 553
           VKDMALPKPYTI
Sbjct: 541 VKDMALPKPYTI 552

BLAST of Moc02g00060 vs. ExPASy TrEMBL
Match: A0A6J1DK02 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 535/552 (96.92%), Postives = 542/552 (98.19%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           M GLMFTVLLCLSAAAMSTVRGEDPYFFFTWN+TYGTI+PLGV QQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLD 180
           GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSHS+LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQ HTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPG+W PEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAA+LLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMALPKPYTI 553
           VKDM LPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of Moc02g00060 vs. ExPASy TrEMBL
Match: A0A5A7TAM1 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00980 PE=3 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 501/553 (90.60%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAA-MSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M GLMFT LLCLSAAA M+TV+GEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH++LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGI DKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMALPKPYTI 553
           LVKDM +PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Moc02g00060 vs. ExPASy TrEMBL
Match: A0A5A7TDG2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001010 PE=3 SV=1)

HSP 1 Score: 1074.3 bits (2777), Expect = 2.1e-310
Identity = 501/553 (90.60%), Postives = 530/553 (95.84%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAA-MSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M  LMFT LLCLSAAA M+TVRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH++LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGI DKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMALPKPYTI 553
           LVKDM +PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Moc02g00060 vs. ExPASy TrEMBL
Match: A0A5A7T9Z0 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001230 PE=3 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 4.1e-310
Identity = 498/553 (90.05%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMST-VRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M GLMFT LLCLSAAAM+T VRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MAGLMFTTLLCLSAAAMTTMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NST+NNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSH++LK FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLKTSLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDY+SLD+HVGQCFSVLVTA+QEPKDYY++ASTRF K+ LVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYKSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKSALVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           NGKGPASPE+P AP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 NGKGPASPELPNAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFG+ DKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGVTDKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VN TFRNF+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W PEKR +YNLLDAVSRHTIQ
Sbjct: 421 VNTTFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRSDYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSEL ENRYLGQQLY SV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELAENRYLGQQLYMSVQSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMALPKPYTI 553
           LVKD+ LPKPYTI
Sbjct: 541 LVKDLPLPKPYTI 553

BLAST of Moc02g00060 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 905.2 bits (2338), Expect = 2.6e-263
Identity = 407/549 (74.13%), Postives = 468/549 (85.25%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           G  +    L L+AAA   VR EDPYF   WNVTYGT++PLGV QQ ILINGQFPGPN+NS
Sbjct: 3   GVKLLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNS 62

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTNYTYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 122 SFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDS 181
           S+FYYPSTAMHR+AGGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK FLDS
Sbjct: 123 SYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDS 182

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR+L RPDG+LINGK+ KGDG+D PLFT+KP KTY+ R+CN+GLKTSLN R Q H +KLV
Sbjct: 183 GRTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLV 242

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQC+  ++TANQE KDYY++AS+RF K+V+   G++RY  G
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGG 302

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
           KGPAS ++P  P GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  
Sbjct: 303 KGPASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 362

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVN 421
           KVDGKLRYA+NGVSH DPETPLKLAEYFG+ DKVFKYD+I+D+   E   ++ + PNV+N
Sbjct: 363 KVDGKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLN 422

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            T R F+E+VFENHEKS+QSWHL+GYSFFAVA+EPG W PEKRKNYNLLDAVSRHT+QV+
Sbjct: 423 ITHRTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 482

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PK WAA+LLT DN GMWN+RSE +E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLV
Sbjct: 483 PKCWAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 542

Query: 542 KDMALPKPY 551
           K    P PY
Sbjct: 543 KGTPKPNPY 551

BLAST of Moc02g00060 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 897.5 bits (2318), Expect = 5.3e-261
Identity = 402/549 (73.22%), Postives = 464/549 (84.52%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           G  +  V LC++ A +  V+ EDPYF   WNVTYGT +PLGV QQ ILINGQFPGPNINS
Sbjct: 4   GVKLLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 63

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN+++NVFNNLDEPFL+ W+GIQHRKN WQDG  GT CPIPPG N+TYHFQ KDQIG
Sbjct: 64  TSNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIG 123

Query: 122 SFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDS 181
           S+FYYP+TAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYT+L  DWYTKSH+ LK FLDS
Sbjct: 124 SYFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDS 183

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR++ RPDG+LINGK+ K DG+D+PLFT+KP KTY+ R+CN+GLK SLN R Q H MKLV
Sbjct: 184 GRTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLV 243

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQCF V+VTA+QEPKDYY+IASTRF K  L   G++RY  G
Sbjct: 244 EMEGSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGG 303

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
           KGPAS ++P AP GWAWSLNQ+R+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  
Sbjct: 304 KGPASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 363

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVN 421
           KVDGKLRYA++GVSH DPETPLKLAEYFG+ DKVFKYDTISD+   +    + + PNV+N
Sbjct: 364 KVDGKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLN 423

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            T R F+E+VFENHE+S+QSWHL+GYSFFAVA+EPG W PEKRKNYNLLDAVSRHT+QV+
Sbjct: 424 ITHRTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 483

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PK WAA+LLT DN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLV
Sbjct: 484 PKCWAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 543

Query: 542 KDMALPKPY 551
           K      PY
Sbjct: 544 KGKPKVNPY 552

BLAST of Moc02g00060 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 873.6 bits (2256), Expect = 8.3e-254
Identity = 402/551 (72.96%), Postives = 467/551 (84.75%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           GG + TVL+CL A+ ++ V   DPYF++TWNVTYGT  PLG+ QQ ILINGQFPGPN+NS
Sbjct: 3   GGRLLTVLVCL-ASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNS 62

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN+VINVFNNLDEPFLL WSG+QHRKNSWQDG+ GT+CPIP GTN+TYHFQ KDQIG
Sbjct: 63  TSNNNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIG 122

Query: 122 SFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDS 181
           S+FYYPSTA+HR AGGFGGLRVNSRLLIPVPYADPEDD T+L  DWY KSH++LKNFLDS
Sbjct: 123 SYFYYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDS 182

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR+L  PDGVLINGK+ K  G + PLFTMKP KTYKYR+CN+G K++LN R QGH MKLV
Sbjct: 183 GRTLGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLV 242

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQCF+VLVTA+Q  K+YY++ASTRF K  +   G++ Y   
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGS 302

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
              AS +IP+AP GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+ N+  
Sbjct: 303 NVQASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKN 362

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVN 421
            V+GK+R+  NGVSH+D ETPLKLAEYFG+ +KVFKY+ I D+  A+   T+TV PNV+N
Sbjct: 363 LVNGKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDEPAAK-ITTLTVEPNVLN 422

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            TFR FVE+VFENHEKS+QS+HL+GYSFFAVA EPG+W PEKR NYNLLDAVSRHT+QV+
Sbjct: 423 ITFRTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVY 482

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PKSW+A+LLT DNAGMWN+RSE  E RYLGQQLY SVLSP +SLRDEYNIP NT LCG+V
Sbjct: 483 PKSWSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIV 542

Query: 542 KDMALPKPYTI 553
           K + LP PYTI
Sbjct: 543 KGLPLPTPYTI 551

BLAST of Moc02g00060 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 861.3 bits (2224), Expect = 4.2e-250
Identity = 396/551 (71.87%), Postives = 465/551 (84.39%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           M G + TVL+CL  + ++ V   DPYFF TWNVTYGT +PLGV Q+ ILINGQFPGPN+N
Sbjct: 1   MEGRLLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNN+VINVFN+LDEPFLL WSGIQHRKN WQDG+ GT+CPIP G N+TYHFQ KDQI
Sbjct: 61  STSNNNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLD 180
           GS+FYYP+T++HR AGGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYT  H++LKNFLD
Sbjct: 121 GSYFYYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGR+L  P+GVLINGK+ K  G +EPLFTMKP KTYKYR+CN+G K++LN R Q H MKL
Sbjct: 181 SGRTLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHV+QNDY SLD+HVGQCFSVLVTANQ  KDYY++ASTRF K  L   G+IRY  
Sbjct: 241 VEMEGSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEG 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
               AS E+P+AP GWAWSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+IK++NS 
Sbjct: 301 SNVQASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSK 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVV 420
           + VDGK+R+  NGVSH+D ETPLKLAEYF + +KVFKY+ I D+  A+    +TV PNV+
Sbjct: 361 SVVDGKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDEPAAK-ITALTVQPNVL 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           N TFR FVEI+FENHEK++QS+HL+GYSFFAVA EPG+W PEKR+NYNLLDAVSRHT+QV
Sbjct: 421 NITFRTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           +PKSW+A+LLT DNAGMWN+RSE  E +YLG+QLY SVLSP +SLRDEYNIP NT LCG+
Sbjct: 481 YPKSWSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGI 540

Query: 541 VKDMALPKPYT 552
           VK + LP  Y+
Sbjct: 541 VKGLPLPAHYS 549

BLAST of Moc02g00060 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 636.7 bits (1641), Expect = 1.7e-182
Identity = 302/535 (56.45%), Postives = 385/535 (71.96%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSN 64
           M T LL L + A +    EDPY FF W+VTYG I+PLGV QQGILING+FPGP+I S +N
Sbjct: 11  MITTLLFLISLAFA----EDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITN 70

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
           +N++INVFN+LDEPFLL W+GI++ KNS+QDG+ GT CPIPPG NYTY  QVKDQIGSF+
Sbjct: 71  DNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFY 130

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSSLKNFLDSGRS 184
           Y+PS   H+AAGGFGG+R++SR LIPVP+  P DDYT+L GDWY  +H  LK  LD+G  
Sbjct: 131 YFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGK 190

Query: 185 LARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEME 244
           L  PDG+LING+++           ++P KTY+ R+ N+GL+ SLN R Q HTMKLVE+E
Sbjct: 191 LPLPDGILINGRSSGA------TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVE 250

Query: 245 GSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGP 304
           G + +QN + SLD+HVGQ +SVL+TA+Q  KDYY++ S+RFT  +L   G++ Y+N   P
Sbjct: 251 GRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAP 310

Query: 305 ASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVD 364
            S  IP+ P   +WS NQ R  R NLTAS  RPNPQGSY YG INITRTI++ N+   ++
Sbjct: 311 VSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIE 370

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGIVDKVFKYDTISDDGRAEGANTVTVAPNVVNATF 424
           GK RYA+N  S    +TPLKL +YF I D V+K  +ISD     GA  +    +V+ A F
Sbjct: 371 GKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ-PTNGA--IFPTTSVMQADF 430

Query: 425 RNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPKS 484
           R FVE++FEN E  +QSWHL+GYSF+ V +E GKW+P  RK YNL DA+ R TIQV+P+S
Sbjct: 431 RAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRS 490

Query: 485 WAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           W A+ + LDN GMWN+RSE+ E +YLGQQ Y  V + + SLRDEY IP N LLCG
Sbjct: 491 WTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153757.10.0e+0098.55L-ascorbate oxidase homolog [Momordica charantia][more]
XP_022153749.10.0e+0096.92L-ascorbate oxidase homolog [Momordica charantia][more]
KAA0040123.10.0e+0090.60L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
KAA0040126.14.2e-31090.60L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
KAA0040130.18.5e-31090.05L-ascorbate oxidase-like protein [Cucumis melo var. makuwa] >TYK21751.1 L-ascorb... [more]
Match NameE-valueIdentityDescription
Q006242.2e-25974.09L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291628.1e-23868.56L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU402.9e-15547.32Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q9FHN62.3e-14745.72Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q8VXX51.5e-14646.24Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1DJT40.0e+0098.55L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021199 PE=3 ... [more]
A0A6J1DK020.0e+0096.92L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 ... [more]
A0A5A7TAM10.0e+0090.60L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TDG22.1e-31090.60L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7T9Z04.1e-31090.05L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT3G13390.12.6e-26374.13SKU5 similar 11 [more]
AT1G55570.15.3e-26173.22SKU5 similar 12 [more]
AT3G13400.18.3e-25472.96SKU5 similar 13 [more]
AT1G55560.14.2e-25071.87SKU5 similar 14 [more]
AT1G21860.11.7e-18256.45SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 346..548
e-value: 2.2E-34
score: 120.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 28..147
e-value: 2.6E-33
score: 116.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 156..336
e-value: 3.0E-35
score: 124.1
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 155..337
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 23..172
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 348..538
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 32..146
e-value: 1.9E-40
score: 137.6
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 385..520
e-value: 3.3E-24
score: 85.2
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 158..298
e-value: 7.5E-39
score: 133.4
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 4..543
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 4..543
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 158..298
e-value: 1.18959E-81
score: 249.627

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g00060.1Moc02g00060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity