Moc01g33180 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g33180
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionalpha-1,2-Mannosidase
Locationchr1: 23313062 .. 23318583 (-)
RNA-Seq ExpressionMoc01g33180
SyntenyMoc01g33180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGTTCTCTACCCTACTCTGTCAAGGATGTCCATTACGACAATGCTAAATTTCGACAGAGACCATACTTCAAGGTCTGTCTATGCTAGATCTTGACTTATTGCACCATTTCGATTTGGTGGAATCTGATTTCTTATCGTGCATTACGTGTGTGCAAACTTTTTTTTCCCTTGACGATGGTATAGTTTTCGATGGTTGATGTTTGGATGAATTGACAATTACATTTTAGCAATCAGTATTTCTTAAACTTTGATTGTACCTGGAAGGATTTTAGGCTTTTGATGTGCAATTATACTTGCTAAGGCCATTTACTTTGTTTAAGACTGTGACGTTGGTTCTGGAATGGAGGTGACCAGGGGGTGATGTATTCATAATTAGCGAAGTAATCGTAGAAATGCAAGACTGAGGATGTTTGTGTTTTGCGAAATAATCATGAACATTGCCAGTTTAGATTCTATGCTTGGTAGTTGTGACTAAACTTTTATCTTCAGCATGATGGATGTTAGGTGTTTTCTTATTGATGTGCAATTATAGTACGTTAACGTATTGTAGGTAGTCGGTTATACCAAAAAAAAAAAAAAAAAACTCTTGATGTCTCCTAGGGTCCGGACTTTGAGGCAGGCGCGGGTTCCCTAGGCACAGGGGAGTAAAGCTCCGACTCCTGGCTATTAAAAAAAAAAGGCCAGTCTTCTTTTTTAGTCTGCTTATCATTAAGGGACTCTAAAGTAGTGATGTAATAGAGCTAAACATAGGAATACGATAAATTTCTGACTTTCCAGTATTTTTGGATGACTGCCCAAAAACGATGAAAAAACAGAGAAGAGAGCTAAACTAAAAGTGTATTCCAGAATTTTCAAGGAAGAATAAGTTTACGTTTTTTTTTTGACATGCCTGAAATAGACACTGGAGTATGACATTTAGTGACTAGAAATTGTTTCCTCCAAATTCGCCCTTACCTGCTGCGTTGAACCTGACACGTGAGACATGTAACTTTATCTCTAAAATACTCATCTCGGGTTGTTGTGAGATCATTGTTATTAGTAACCCTGGGAATTATATACAGTTTTTTATACTGAATATAGTGACCCAATCATTTATTTTTCTATTTATCATGTCATATCCTAGATACATGAAGATTTATATTATGGCAGTACAAAACTCTGATCATTAGATCTTATTAAAAAGGAATTCTGATTATTAGAATAAATTCTTATTGGTTACAGACTAAATGTTTCAGAATCTTTCATTTTAATTTAAGTACCAGCCTATAGTAACTTGATATGGGTTACCATTGTTTGTTTTGAGTTATGTCTTAACAATATCATCTTGTTTTCTCAGGTGATGACTCAGAATTTACGCACTGGTAGCGCAAAACGTGATTATCTAAGTTGCAGTACGGGGAAGTTTCTGGTATTACTGTTGATCTTTGGCTTAGCATATCTTTTATTGACAAGTACAACTCCTGTCCATTCTGCTCCCCATCGACTAGCCAAAGTCAGTGAGAATAATGAGAAAGGAGAGTTTATTAATAGCGGTGGCATCAGCTTCAGGAACTTATGGAGGAGAGCTCCAAGACTTCCCCCTCGATTACCACCAGACGAAAAAGGTAGCAACACTAGCTACAACATTGATCCAAATCTATTAATCTCTGAGTCGTTATGGATTTCAAGGCAACAGAAAGTTAAGGATGCTTTTATCCATGCATGGTCCGGCTATAAGAGATATGCAATGGGGCATGACGAACTCATGCCACTGAGCCAGCAAGGAACTGATGGGTTAGGAGGTCTAGGTGCTACGGTTGTGGATGCCCTTGACACTGCCATGATTATGGGTGATAGTGAAATTGTCTCTGAAGCAGGCTCGTGGGTTGAAAAACACCTTTTGGATAGAATTAATGAGAAAGGTCAAGTCAATTTATTTGAAACTACCATACGTGTTTTAGGTGGTCTTTTAAGTGCATACCACTTGAGAGCTGGAGAATTTGGGAAGACCTTTGAAAATGAAGGGCCTAAACCAGATGTTTATCTAGAAATTGCTAGGAACTTGGCTGATCGTTTGCTTAGTGCCTTTACCTCAAGTCCAACCACTATTCCTCTCAGTGATGTTGTTTTAAAAGATTCCTCGGCTCATGCAGCACCTGGTGGAGTAAGTAGTACATCCGAAGTTTCCACTTTGCAGCTGGAGTTTAATTATCTTAGTACTGTTTCCGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAGCCTTCCAAAAGTGGAAGGTTTGGTTCCCATCTATATTAGGTATGGAGCTTTTATATTTAGCTATAAGAATGGTTACTTTAGTTTAGGCAATATGTCTGTGTCCAACATATGTGTTCAACTTTTTATAATTTTTTTTTTGTTCTGATCTATAGACATATCAGAAAAAAGATATTAGATGACAGTAATGAATTTCATACCATCAACGACGATCGTACGATCTACTTTCATCACAATATGCTAGATTTTTAGTCTTTTAAAAATTTGATGTCTCATCATGTAGTGTTATGTCCATGCTTCTCAGGGTCTCAGATATTGCTTCTCTTGAACCTTTTGGCACGTTGGCAAAATTCTCTTTTGAATCATGAGATGTCATTCTTCTGCAGTCAACAGTGGAAGTTAAATTCCATAGGTCAAAGATATATGAAATAATCACAAATGGTTATGCTTGTATCGGTTTTTTTGTTTTTTTGGGGTACTTCTGGTTTCGTGGCTGTTTGTCCTTTTCGAGCACTAATCCTGAAAAGCTTCACTTTGTATTTCACATTTGATTTTTGAGTTTTGGAAGTTGAAAACACCATCAGTTCTGTTGTTTACGACATGTAGAATGTGGTTTCTTCCGCATTCTAAATCTCTGTGTGCTTTTTAGTTCTTGAAAGGTTCATTTAGTTTGGAGAAAATACCGATGATGACTAGTTTTACTTATTCTTATGTTATTTTCTTCTCAAATGCAGCCCTTATTCTGGTGAATTTAGTGGAGAAACCATAAGACTGGGATCCCGTGGTGATAGCTACTATGAGTATCTTCTGAAAGTGTGGCTTCAGAAGGGAGCAAGAGAAGACAATAATGTCACTTATCTACATGATATGTATGAAGAAGCCATGAAAGGTGTTAGGCATCTTCTTGTTCGGAAGTCAGTTCCGAATGGATTGGTTTTTGTTGGAGAATTGCCTTATGGATCAAATGATGCTTTGAGCCCAAAAATGGATCACCTGGTATGCATTATTTCCTCTTGTATCTAATATCCACTTATCTTGTAGACTGGCTTATGTTATGGTCAAGGAACATGGCTCAAACTAGGAAATAATTTGGTAGATTGTCCGCACATGTAGGAGTTGGATTGATTTTGCTGCATATTAATTCGTAGATGATTTCAAGTTTTGAAATATAATTTGGCATATATGATATAGGATGAGCTATATATGATTGTTGACATGGTATAAGGCATAAACACATCTTTCATTCACCCTCTGGATCAATATTTCTTGTAATATATCAATGGAAATAGTTTCTTTATCTTGTTGACTGTTATCTATTACTCCATGTGCAATAATTGTTGTGATTATGTAATAGATTTGGGATGTTGGGAAATGTCTAGAGCATAGATTCACCTCCTCAGGGACATTATACAAACTTCTTTCCCTGCTGCCCCTATGGCAAGGGAGTACAATAGGCCGCTCCATCTGCTTCTAGAGACAAGTTTTAAGCTTTAGAGTCTCTTTTGGTTTTATCTATTTAATCTAACTATTGAACTATGGCATTTGATAGCAATTTGATTGAACATGACTGTCCTGTTACTAGGAAACTCGAAGCTCGAAACTCTCCCGCAAGCATCCTTCTTAGGTCGGAATTTGGCTTTGGCCTTGCTTGTTCGGTTTTGGCTCATTCTTTTGATTGTCATGTCTGCCCCGTCTACTGGTATAACATAGTTGTATTCTCCAACAGGAAATAAAAAAAGAAAAATCACTTGGCAATGATGTATAAGAAAGTAGTGGAATTCAAATGCCATTGCCAATATAAATATCTTTTTGAATAAATACAATGCGGGAGTAAATCAGGTTATCTGACATCAATTGTGCAAAAAAGGATGCGTCAGTTTACAAGTACTGAGGAAAAGTTTCACCTCTTAAGTTAGCTTTTGGGTGAGAACCTCCAACCCCCATTTGACACAAACAAGTGCCATAAGCGCTCAGACTACAGAAACTCTATGCAAAAACCCAGGGGAACTCAAGTTAATGGTGAGATTGTGAGATCCAACTGAAAGTTTGATAATGTGAGAGGGGATTGTCGAGTTTGTCCATGAAGGGGTTGGATATTGGTTAAGTGTTAAGGAAATTCTCGCCTCATAAATTAGCTTTTGGAGACAGCTTGTTTGGTAATAACCATGAAGTGGTGCAGGGTAAAAAAAAACAAACAAATAGTGGTAGAAGGAATGTATGGGAAAAAGATTTTTTAAAGAAAAATAAAAAGAACCGTCAGTTCGTATTCCACCTGTTCGCATTCTAATGTTTGGACTCAGGTAACTATTTCTTCGATGAGTTAACTAAGCCTGCATATTTTCTACGATAATGTAATGTGATTCCATTTCAAGACTAATTAATTCACAAGCTCACAGCCTGCATATTTGCTTTCCAAGGAATATCTACATTATTGTAAAGCAATGCGACGTAAAATATTTGACTAACAAGCTTGTGCCTGTTTCAGGTATGTTTCTTATCCGGTACTCTTGCTCTCGGTGCTACAAAGGGCATCAGCAAGGAAGAAGCTATGAATAAAAGCTTGCTGAGCTTTGAAGATCTGGAAAATCTAAATCTGGCAGAGGATCTGGCAAAAACTTGCTATGAGATGTATGAAGTAACCTCCACCGGTCTTGCCCCAGAAATAGTTTATTTTCGTACTGAGGTTGGCTCTATGATTTATGACCTCGTCTTTCTTTTTCCCATTTGCTCATGCTGCCCATTAATCTGAGACACTGGGCTCATCATTTGTCCAGGACTTTTCTGAAGAAAATTCTGATGGTGGAAATAAGAACTCGAAGTATGTCAATGACATAATAATCAAGCCACTCGATCGCCACAATCTTTTGCGGCCCGAAACAGTCGAGTCGCTGTTCGTTCTGTATCGTATCACAGGCAACCCAAAGTATGCCTTCCATATTAACTATACTTCCTGTATCGTTCATTAACCGAGTTATATATGATTTACGTGAATGTATCGCAGGTACCGTGCATGGGGTTGGAAGATTTTCGAAGCGTTTGAGAAGCACACGAAGGTTAATAGTGGTGGATATACATCGCTAGATGATGTTACTACAGTTCCTCCGCGAAGGAGGGACAAAATGGAGACATTCTTTTTGGGGGAAACTCTCAAGTATTTATACTTGCTATTTGGGGACAGCTCAACGATGCCATTGAACAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATTGAAGGCATTAGTCAGATAAAATGA

mRNA sequence

ATGTCGAGTTCTCTACCCTACTCTGTCAAGGATGTCCATTACGACAATGCTAAATTTCGACAGAGACCATACTTCAAGGTGATGACTCAGAATTTACGCACTGGTAGCGCAAAACGTGATTATCTAAGTTGCAGTACGGGGAAGTTTCTGGTATTACTGTTGATCTTTGGCTTAGCATATCTTTTATTGACAAGTACAACTCCTGTCCATTCTGCTCCCCATCGACTAGCCAAAGTCAGTGAGAATAATGAGAAAGGAGAGTTTATTAATAGCGGTGGCATCAGCTTCAGGAACTTATGGAGGAGAGCTCCAAGACTTCCCCCTCGATTACCACCAGACGAAAAAGGTAGCAACACTAGCTACAACATTGATCCAAATCTATTAATCTCTGAGTCGTTATGGATTTCAAGGCAACAGAAAGTTAAGGATGCTTTTATCCATGCATGGTCCGGCTATAAGAGATATGCAATGGGGCATGACGAACTCATGCCACTGAGCCAGCAAGGAACTGATGGGTTAGGAGGTCTAGGTGCTACGGTTGTGGATGCCCTTGACACTGCCATGATTATGGGTGATAGTGAAATTGTCTCTGAAGCAGGCTCGTGGGTTGAAAAACACCTTTTGGATAGAATTAATGAGAAAGGTCAAGTCAATTTATTTGAAACTACCATACGTGTTTTAGGTGGTCTTTTAAGTGCATACCACTTGAGAGCTGGAGAATTTGGGAAGACCTTTGAAAATGAAGGGCCTAAACCAGATGTTTATCTAGAAATTGCTAGGAACTTGGCTGATCGTTTGCTTAGTGCCTTTACCTCAAGTCCAACCACTATTCCTCTCAGTGATGTTGTTTTAAAAGATTCCTCGGCTCATGCAGCACCTGGTGGAGTAAGTAGTACATCCGAAGTTTCCACTTTGCAGCTGGAGTTTAATTATCTTAGTACTGTTTCCGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAGCCTTCCAAAAGTGGAAGGTTTGGTTCCCATCTATATTAGCCCTTATTCTGGTGAATTTAGTGGAGAAACCATAAGACTGGGATCCCGTGGTGATAGCTACTATGAGTATCTTCTGAAAGTGTGGCTTCAGAAGGGAGCAAGAGAAGACAATAATGTCACTTATCTACATGATATGTATGAAGAAGCCATGAAAGGTGTTAGGCATCTTCTTGTTCGGAAGTCAGTTCCGAATGGATTGGTTTTTGTTGGAGAATTGCCTTATGGATCAAATGATGCTTTGAGCCCAAAAATGGATCACCTGGTATGTTTCTTATCCGGTACTCTTGCTCTCGGTGCTACAAAGGGCATCAGCAAGGAAGAAGCTATGAATAAAAGCTTGCTGAGCTTTGAAGATCTGGAAAATCTAAATCTGGCAGAGGATCTGGCAAAAACTTGCTATGAGATGTATGAAGTAACCTCCACCGGTCTTGCCCCAGAAATAGTTTATTTTCGTACTGAGGACTTTTCTGAAGAAAATTCTGATGGTGGAAATAAGAACTCGAAGTATGTCAATGACATAATAATCAAGCCACTCGATCGCCACAATCTTTTGCGGCCCGAAACAGTCGAGTCGCTGTTCGTTCTGTATCGTATCACAGGCAACCCAAAGTACCGTGCATGGGGTTGGAAGATTTTCGAAGCGTTTGAGAAGCACACGAAGGTTAATAGTGGTGGATATACATCGCTAGATGATGTTACTACAGTTCCTCCGCGAAGGAGGGACAAAATGGAGACATTCTTTTTGGGGGAAACTCTCAAGTATTTATACTTGCTATTTGGGGACAGCTCAACGATGCCATTGAACAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATTGAAGGCATTAGTCAGATAAAATGA

Coding sequence (CDS)

ATGTCGAGTTCTCTACCCTACTCTGTCAAGGATGTCCATTACGACAATGCTAAATTTCGACAGAGACCATACTTCAAGGTGATGACTCAGAATTTACGCACTGGTAGCGCAAAACGTGATTATCTAAGTTGCAGTACGGGGAAGTTTCTGGTATTACTGTTGATCTTTGGCTTAGCATATCTTTTATTGACAAGTACAACTCCTGTCCATTCTGCTCCCCATCGACTAGCCAAAGTCAGTGAGAATAATGAGAAAGGAGAGTTTATTAATAGCGGTGGCATCAGCTTCAGGAACTTATGGAGGAGAGCTCCAAGACTTCCCCCTCGATTACCACCAGACGAAAAAGGTAGCAACACTAGCTACAACATTGATCCAAATCTATTAATCTCTGAGTCGTTATGGATTTCAAGGCAACAGAAAGTTAAGGATGCTTTTATCCATGCATGGTCCGGCTATAAGAGATATGCAATGGGGCATGACGAACTCATGCCACTGAGCCAGCAAGGAACTGATGGGTTAGGAGGTCTAGGTGCTACGGTTGTGGATGCCCTTGACACTGCCATGATTATGGGTGATAGTGAAATTGTCTCTGAAGCAGGCTCGTGGGTTGAAAAACACCTTTTGGATAGAATTAATGAGAAAGGTCAAGTCAATTTATTTGAAACTACCATACGTGTTTTAGGTGGTCTTTTAAGTGCATACCACTTGAGAGCTGGAGAATTTGGGAAGACCTTTGAAAATGAAGGGCCTAAACCAGATGTTTATCTAGAAATTGCTAGGAACTTGGCTGATCGTTTGCTTAGTGCCTTTACCTCAAGTCCAACCACTATTCCTCTCAGTGATGTTGTTTTAAAAGATTCCTCGGCTCATGCAGCACCTGGTGGAGTAAGTAGTACATCCGAAGTTTCCACTTTGCAGCTGGAGTTTAATTATCTTAGTACTGTTTCCGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAGCCTTCCAAAAGTGGAAGGTTTGGTTCCCATCTATATTAGCCCTTATTCTGGTGAATTTAGTGGAGAAACCATAAGACTGGGATCCCGTGGTGATAGCTACTATGAGTATCTTCTGAAAGTGTGGCTTCAGAAGGGAGCAAGAGAAGACAATAATGTCACTTATCTACATGATATGTATGAAGAAGCCATGAAAGGTGTTAGGCATCTTCTTGTTCGGAAGTCAGTTCCGAATGGATTGGTTTTTGTTGGAGAATTGCCTTATGGATCAAATGATGCTTTGAGCCCAAAAATGGATCACCTGGTATGTTTCTTATCCGGTACTCTTGCTCTCGGTGCTACAAAGGGCATCAGCAAGGAAGAAGCTATGAATAAAAGCTTGCTGAGCTTTGAAGATCTGGAAAATCTAAATCTGGCAGAGGATCTGGCAAAAACTTGCTATGAGATGTATGAAGTAACCTCCACCGGTCTTGCCCCAGAAATAGTTTATTTTCGTACTGAGGACTTTTCTGAAGAAAATTCTGATGGTGGAAATAAGAACTCGAAGTATGTCAATGACATAATAATCAAGCCACTCGATCGCCACAATCTTTTGCGGCCCGAAACAGTCGAGTCGCTGTTCGTTCTGTATCGTATCACAGGCAACCCAAAGTACCGTGCATGGGGTTGGAAGATTTTCGAAGCGTTTGAGAAGCACACGAAGGTTAATAGTGGTGGATATACATCGCTAGATGATGTTACTACAGTTCCTCCGCGAAGGAGGGACAAAATGGAGACATTCTTTTTGGGGGAAACTCTCAAGTATTTATACTTGCTATTTGGGGACAGCTCAACGATGCCATTGAACAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATTGAAGGCATTAGTCAGATAAAATGA

Protein sequence

MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAYLLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTSYNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGEFGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRLGSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK
Homology
BLAST of Moc01g33180 vs. NCBI nr
Match: XP_022146514.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Momordica charantia])

HSP 1 Score: 1281.9 bits (3316), Expect = 0.0e+00
Identity = 635/635 (100.00%), Postives = 635/635 (100.00%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS
Sbjct: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY
Sbjct: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF
Sbjct: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 636
           YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK
Sbjct: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 635

BLAST of Moc01g33180 vs. NCBI nr
Match: XP_038882608.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X1 [Benincasa hispida])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 578/635 (91.02%), Postives = 607/635 (95.59%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQR YFKVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTPVH  P+R+AKV ENNEKGEF N+GGI+FRNLWRRAPRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPVH-GPNRVAKVGENNEKGEFKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+ DPN LISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQ GTDGLGGLGATV
Sbjct: 121 YHFDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSWVEKHLLDRI EKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GK FENEGPKP VYLEIA+NLADRLLSAFTSSPT IPL DVVLKDSSAHAAPGG SSTS
Sbjct: 241 YGKRFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISP +G+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPQTGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQK  RE+NN TYLHDMYEEAMKGV+HLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKRRRENNNFTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS+ ++SPKMDHLVCFLSGTLALGATKGI+KEEAM+KSLLSF+DLENLNLAEDLAKTCY
Sbjct: 421 YGSDSSISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCY 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVTSTGLAPEIVYF TE++SE+  DGGNKNSKY+ DIIIKPLDRHNL+RPETVESLF
Sbjct: 481 EMYEVTSTGLAPEIVYFHTEEYSEQGLDGGNKNSKYIGDIIIKPLDRHNLMRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITGNPKYRAWGW+IFEAFE+HTKV+SGGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 636
           YLYLLFGDSST+PL+KYVFNTEAHPLPIEG  QIK
Sbjct: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 634

BLAST of Moc01g33180 vs. NCBI nr
Match: XP_023517941.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. pepo] >XP_023517942.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. pepo] >XP_023517943.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 566/629 (89.98%), Postives = 603/629 (95.87%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQR YFKVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP    P+RL KVSENN+KGEF ++GGI+FRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNRLDKVSENNQKGEFKSNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSWVEKHLLDRI EKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTFENEGPKP VYLE A+NLADRLLSAFTSSPT IPL DVVL+DSSAHAAPGG+SSTS
Sbjct: 241 YGKTFENEGPKPAVYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYS+AAMKVLEHMKSLPK+EGLVPIYISP SG+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSMAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGARE++N+TYLHDMYEEAMKGV+HLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENHNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS++A+SPKMDHLVCFL GTLALGATKGISKEEAM KSLLSFEDLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEEAMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVT+TGLAPEIVYF TE++SE   DGGNKN+KY++DIIIKP DRHNLLRPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+PKYRAWGW+IFEAFE+HTKVN+GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIE 630
           YLYLLFGD+ST+PL+KYVFNTEAHPLPIE
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIE 629

BLAST of Moc01g33180 vs. NCBI nr
Match: XP_023003322.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita maxima] >XP_023003323.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita maxima] >XP_023003324.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita maxima])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 566/629 (89.98%), Postives = 602/629 (95.71%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQR YFKVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLFTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP    P++L KV ENN+KGEF N+GGI+FRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNQLDKVRENNQKGEFKNNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSWVEKHLLDRI EKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTFENEGPKP VYLE A+NLADRLLSAFTSSPT IPL DVVL+DSSAHAAPGG+SSTS
Sbjct: 241 YGKTFENEGPKPAVYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMKSLPK+EGLVPIYISP SG+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGARE+NN+TYLHDMYEEAMKGV+HLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENNNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS++A+SPKMDHLVCFL GTLALGATKGISKEE+M KSLLSFEDLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEESMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVT+TGLAPEIVYF TE++SE   DGGNKN+KY++DIIIKP DRHNLLRPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+PKYRAWGW+IFEAFE+HTKVN+GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIE 630
           YLYLLFGD+ST+PL+KYVFNTEAHPLPIE
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIE 629

BLAST of Moc01g33180 vs. NCBI nr
Match: XP_022926714.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita moschata] >XP_022926715.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita moschata] >XP_022926716.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita moschata])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 565/629 (89.83%), Postives = 601/629 (95.55%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQR YFKVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP    P+RL KVSENN+KGEF ++GGI+FRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNRLDKVSENNQKGEFKSNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSWVEKHLLDRI EKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTFENEGP P  YLE A+NLADRLLSAFTSSPT IPL DVVL+DSSAHAAPGG+SSTS
Sbjct: 241 YGKTFENEGPTPAAYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMKSLPK+EGLVPIYISP SG+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGARE++N+TYLHDMYEEAMKGV+HLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENHNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS++A+SPKMDHLVCFL GTLALGATKGISKEEAM KSLLSFEDLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEEAMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVT+TGLAPEIVYF TE++SE   DGGNKN+KY++DIIIKP DRHNLLRPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+PKYRAWGW+IFEAFE+HTKVN+GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIE 630
           YLYLLFGD+ST+PL+KYVFNTEAHPLPIE
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIE 629

BLAST of Moc01g33180 vs. ExPASy Swiss-Prot
Match: Q93Y37 (Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana OX=3702 GN=MNS3 PE=1 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 7.1e-262
Identity = 451/632 (71.36%), Postives = 522/632 (82.59%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYSVKD+HYDNAKFR R   KV +Q+L T S KR+Y SCSTGKFL+L+L FG+A 
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDE---KGS 120
           L+L S +P  S           NEKG+    GG+    L R+ PRLPPRL PDE   +GS
Sbjct: 61  LMLMSKSPNESG---------LNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGS 120

Query: 121 NTSYNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLG 180
           +T+ +   N   S+  W +RQQ VK+AF HAWSGY++YAMG+DELMP+SQ+G DGLGGLG
Sbjct: 121 STNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLG 180

Query: 181 ATVVDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLR 240
           ATVVDALDTAMIMG   IVSEAGSWVE HLL+RI++KGQVNLFETTIRVLGGLLSAYHL 
Sbjct: 181 ATVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLS 240

Query: 241 AGEFGK-TFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGV 300
            GE G     + GPKP +YL IA++LADRLLSAFTSSPT +P  DV+L +S+AH APGG 
Sbjct: 241 GGEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGA 300

Query: 301 SSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGE 360
           SST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K+LPK EGLVPIYISP +G+F GE
Sbjct: 301 SSTAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGE 360

Query: 361 TIRLGSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFV 420
            IRLGSRGDSYYEYL+KVWLQ+GA+ ++N TYLHDMY EAMKGVRHLLV+ S+P GLVFV
Sbjct: 361 NIRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFV 420

Query: 421 GELPYGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLA 480
           GELPYGS    SPKMDHLVCFL GTLALGATKG++KE+A+ ++LLSFEDLENL LAEDLA
Sbjct: 421 GELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLA 480

Query: 481 KTCYEMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETV 540
           KTC+EMYEVT+TGLAPEI YF T+D++E+  DGGNK+S Y NDIIIKP DRHNLLRPETV
Sbjct: 481 KTCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETV 540

Query: 541 ESLFVLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLG 600
           ESLFVLYRIT + KYR  GW+IFEAFEK+TKV SGGYTSLDDVT VPP RRDKMETFFLG
Sbjct: 541 ESLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLG 600

Query: 601 ETLKYLYLLFGDSSTMPLNKYVFNTEAHPLPI 629
           ETLKYLYLLFGD S +PL+K+VFNTEAHPLPI
Sbjct: 601 ETLKYLYLLFGDDSVIPLDKFVFNTEAHPLPI 620

BLAST of Moc01g33180 vs. ExPASy Swiss-Prot
Match: Q8J0Q0 (Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans OX=5476 GN=MNS1 PE=3 SV=2)

HSP 1 Score: 401.4 bits (1030), Expect = 1.9e-110
Identity = 240/509 (47.15%), Postives = 317/509 (62.28%), Query Frame = 0

Query: 131 ESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTD-GLGGLGATVVDALDTAMI 190
           +S W   Q +V+ A + +W  Y++Y  G+D   P+ Q+G + G   LG  +VD+LDT MI
Sbjct: 32  QSKWERAQSEVRQAILDSWHTYEKYGWGYDVYHPIKQEGENMGPKPLGWMIVDSLDTLMI 91

Query: 191 MGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGEFGKTFENEG 250
           M   E VS A  W+ K+ LD   +   VN FETTIR+LGGLLSAYH         F N+ 
Sbjct: 92  MDCPEEVSRARDWI-KNDLDYTFDY-NVNTFETTIRMLGGLLSAYH---------FSND- 151

Query: 251 PKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSA--HAAPGGVSSTSEVSTLQL 310
              DVYL+ A  LA+ L  A+  SP+ IP S V LK      +    G SST+E +T+QL
Sbjct: 152 ---DVYLDKAVQLANALHGAY-DSPSGIPYSSVNLKSGKGIKNHVDNGASSTAEAATVQL 211

Query: 311 EFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRLGSRGDSY 370
           E  YLS ++G+  +   A KV++ ++S    +GLVPIY++P +G++ G  IRLGSRGDSY
Sbjct: 212 EMKYLSKLTGEILWWNLAEKVMQVLESNKPQDGLVPIYVNPDTGKYQGHLIRLGSRGDSY 271

Query: 371 YEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDAL 430
           YEYLLK +LQ   +E        DMY E+++GV+  LV  S P+GL F+GEL  G    L
Sbjct: 272 YEYLLKQYLQTNKQE----LVYWDMYRESVEGVKKHLVSDSYPSGLTFIGELDNGIGGKL 331

Query: 431 SPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMY-EVT 490
           S KMDHLVCF  G LALGAT G++  EA +    + E   +  L E+L  TCY+MY +V+
Sbjct: 332 STKMDHLVCFYGGLLALGATGGLTLNEAQSLKSWNEEREADFKLGEELTYTCYKMYHDVS 391

Query: 491 STGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRIT 550
            TGL+PEIV F             N+++    D IIKPLDRHNL RPETVESLF LYR+T
Sbjct: 392 PTGLSPEIVVF-------------NEDTSKSKDFIIKPLDRHNLQRPETVESLFYLYRLT 451

Query: 551 GNPKYRAWGWKIFEAFEKHTK-VNSGG---YTSLDDVTTVP----PRRRDKMETFFLGET 610
           G+ KYR  G++IF+ F K+TK VNS G   ++SL DVT+      P+ +D  E+F+  ET
Sbjct: 452 GDVKYREMGYEIFQNFIKYTKVVNSEGEVSFSSLSDVTSFDSNGLPKFKDNTESFWWAET 507

Query: 611 LKYLYLLFGDSSTMPLNKYVFNTEAHPLP 628
           LKYLYLLF D++ +PL  YVFNTEAHP P
Sbjct: 512 LKYLYLLFDDTNKIPLTDYVFNTEAHPFP 507

BLAST of Moc01g33180 vs. ExPASy Swiss-Prot
Match: Q9UKM7 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sapiens OX=9606 GN=MAN1B1 PE=1 SV=2)

HSP 1 Score: 400.2 bits (1027), Expect = 4.3e-110
Identity = 242/535 (45.23%), Postives = 321/535 (60.00%), Query Frame = 0

Query: 101 RRA--PRLPPRLPPDEKGSNTSYNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMG 160
           RRA  P  PP  P   +G+    N              RQ+ V D F+HAW GY+++A G
Sbjct: 224 RRAEVPTKPPLPPARTQGTPVHLNY-------------RQKGVIDVFLHAWKGYRKFAWG 283

Query: 161 HDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVN 220
           HDEL P+S+  ++   GLG T++DALDT  I+G  +   EA  WV K L     +   VN
Sbjct: 284 HDELKPVSRSFSEWF-GLGLTLIDALDTMWILGLRKEFEEARKWVSKKL--HFEKDVDVN 343

Query: 221 LFETTIRVLGGLLSAYHLRAGEFGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIP 280
           LFE+TIR+LGGLLSAYHL                 ++L  A +  +RL+ AF  +P+ IP
Sbjct: 344 LFESTIRILGGLLSAYHLSG-------------DSLFLRKAEDFGNRLMPAF-RTPSKIP 403

Query: 281 LSDVVLKDSSAHAAPGGVSST-SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLP- 340
            SDV +    AH       ST +EV+++QLEF  LS ++GD K+  A  KV +H+  L  
Sbjct: 404 YSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSG 463

Query: 341 KVEGLVPIYISPYSGEFSG-ETIRLGSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEE 400
           K +GLVP++I+ +SG F+      LG+R DSYYEYLLK W+Q G +E    T L + Y E
Sbjct: 464 KKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQE----TQLLEDYVE 523

Query: 401 AMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMDHLVCFLSGTLALGATKGISKEEA 460
           A++GVR  L+R S P+ L FVGEL +G     S KMDHLVCFL GTLALG   G+     
Sbjct: 524 AIEGVRTHLLRHSEPSKLTFVGELAHG---RFSAKMDHLVCFLPGTLALGVYHGL----- 583

Query: 461 MNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSK 520
                       ++ LA++L +TCY+M     TGL+PEIV+F            G +   
Sbjct: 584 ---------PASHMELAQELMETCYQMNRQMETGLSPEIVHFNL------YPQPGRR--- 643

Query: 521 YVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTS 580
              D+ +KP DRHNLLRPETVESLF LYR+TG+ KY+ WGW+I ++F + T+V SGGY+S
Sbjct: 644 ---DVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSS 695

Query: 581 LDDV-TTVPPRRRDKMETFFLGETLKYLYLLFGDS-STMPLNKYVFNTEAHPLPI 629
           +++V     P  RDKME+FFLGETLKYL+LLF D  + + L+ YVFNTEAHPLPI
Sbjct: 704 INNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPI 695

BLAST of Moc01g33180 vs. ExPASy Swiss-Prot
Match: B2GUY0 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus norvegicus OX=10116 GN=Man1b1 PE=2 SV=2)

HSP 1 Score: 392.1 bits (1006), Expect = 1.2e-107
Identity = 234/540 (43.33%), Postives = 326/540 (60.37%), Query Frame = 0

Query: 100 WRRAPRLPPRLPPDEKGSNTSYNIDPNLLISESLW------ISRQQKVKDAFIHAWSGYK 159
           WR A  + P    +        +I P L ++  +W        RQ+ V +AF+HAW GY+
Sbjct: 166 WRGAV-IEPEQATEPPSKRAEASIKP-LFLASRIWKEPAPPNERQKGVIEAFLHAWKGYQ 225

Query: 160 RYAMGHDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIVSEAGSWVEKHLLDRINE 219
           ++A GHDEL P+S+  ++   GLG T++DALDT  I+G  +   EA  WV ++L     +
Sbjct: 226 KFAWGHDELKPVSKTFSEWF-GLGLTLIDALDTMWILGLKQEFKEARKWVSENL--DFQK 285

Query: 220 KGQVNLFETTIRVLGGLLSAYHLRAGEFGKTFENEGPKPDVYLEIARNLADRLLSAFTSS 279
              VNLFE+TIR+LGGLLSAYHL                 ++L  A +  +RL+ AFT +
Sbjct: 286 NVDVNLFESTIRILGGLLSAYHLSG-------------DSLFLSKAEDFGNRLMPAFT-T 345

Query: 280 PTTIPLSDVVLKDSSAHAAPGGVSST-SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHM 339
           P+ IP SDV +    AH+      ST +EV+++QLEF  LS ++G  K+  A  +V +H+
Sbjct: 346 PSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEFRELSRLTGIKKFQEAVEEVTKHI 405

Query: 340 KSLP-KVEGLVPIYISPYSGEFSGETI-RLGSRGDSYYEYLLKVWLQKGAREDNNVTYLH 399
            SL  K +GLVP++I+  SG F+   +  LG+R DSYYEYLLK W+Q G +E    T L 
Sbjct: 406 HSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSYYEYLLKQWIQGGKKE----TQLL 465

Query: 400 DMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMDHLVCFLSGTLALGATKGI 459
           + Y  A++G++  L+R+S P  L FVGEL +G     S KMDHLVCFL GTLALG   G+
Sbjct: 466 EDYVRAIEGIKAHLLRQSQPRKLTFVGELAHG---RFSAKMDHLVCFLPGTLALGVHHGL 525

Query: 460 SKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFRTEDFSEENSDGG 519
                           ++++LA  L +TCY+M +   TGL+PEI +F     ++      
Sbjct: 526 --------------PADHMDLARALMETCYQMNQQMETGLSPEIAHFNMYPRADH----- 585

Query: 520 NKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPKYRAWGWKIFEAFEKHTKVNS 579
                   D+ +KP DRHNLLRPETVESLF LYR+T + KY+ WGW+I ++F K+T+V S
Sbjct: 586 -------KDVEVKPADRHNLLRPETVESLFYLYRVTKDRKYQDWGWEILQSFNKYTRVPS 645

Query: 580 GGYTSLDDV-TTVPPRRRDKMETFFLGETLKYLYLLFGDS-STMPLNKYVFNTEAHPLPI 629
           GGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF D    + L+  VFNTEAHPLPI
Sbjct: 646 GGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFSDDLELLGLDTCVFNTEAHPLPI 653

BLAST of Moc01g33180 vs. ExPASy Swiss-Prot
Match: A2AJ15 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musculus OX=10090 GN=Man1b1 PE=1 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.2e-106
Identity = 226/513 (44.05%), Postives = 317/513 (61.79%), Query Frame = 0

Query: 127 LLISESLW------ISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 186
           L+++  +W        RQ+ V +AF+HAW GY+++A GHDEL P+S+  ++   GLG T+
Sbjct: 192 LVLASKIWKEPAPPNERQKGVIEAFLHAWKGYQKFAWGHDELKPVSKTFSEWF-GLGLTL 251

Query: 187 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 246
           +DALDT  I+G  +   +A  WV ++L     +   VNLFE+TIR+LGGLLS YHL    
Sbjct: 252 IDALDTMWILGLKQEFKQARKWVSENL--DFQKNVDVNLFESTIRILGGLLSTYHLSG-- 311

Query: 247 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSST- 306
                        ++L  A +   RL+ AFT +P+ IP SDV +    AH+      ST 
Sbjct: 312 -----------DSLFLTKAEDFGKRLMPAFT-TPSKIPYSDVNIGTGFAHSPQWTSDSTV 371

Query: 307 SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLP-KVEGLVPIYISPYSGEFSGETI 366
           +EV+++QLEF  LS ++G  K+  A  +V +H+ SL  K +GLVP++I+  SG F+   +
Sbjct: 372 AEVTSIQLEFRELSRLTGIKKFQEAVEEVTKHIHSLSGKKDGLVPMFINTNSGLFTHPGV 431

Query: 367 -RLGSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVG 426
             LG+R DSYYEYLLK W+Q G +E    T L + Y +A++G++  L+R+S P  L FVG
Sbjct: 432 FTLGARADSYYEYLLKQWIQGGKKE----TQLLEDYVKAIEGIKAHLLRQSQPRKLTFVG 491

Query: 427 ELPYGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAK 486
           EL +G     S KMDHLVCFL GTLALG   G+                ++++LA  L +
Sbjct: 492 ELAHG---RFSAKMDHLVCFLPGTLALGVHHGL--------------PADHMDLARALME 551

Query: 487 TCYEMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVE 546
           TCY+M +   TGL+PEI +F     ++              D+ +KP DRHNLLRPETVE
Sbjct: 552 TCYQMNQQMETGLSPEIAHFNMYPRADH------------KDVEVKPADRHNLLRPETVE 611

Query: 547 SLFVLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDV-TTVPPRRRDKMETFFLG 606
           SLF LYR+T + KY+ WGW+I ++F K+T+V SGGY+S+++V  +  P  RDKME+FF+G
Sbjct: 612 SLFYLYRVTRDRKYQDWGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVG 654

Query: 607 ETLKYLYLLFGDS-STMPLNKYVFNTEAHPLPI 629
           ETLKYLYLLF D    + L+  VFNTEAHPLPI
Sbjct: 672 ETLKYLYLLFSDDLELLSLDSCVFNTEAHPLPI 654

BLAST of Moc01g33180 vs. ExPASy TrEMBL
Match: A0A6J1CYC2 (alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111015717 PE=3 SV=1)

HSP 1 Score: 1281.9 bits (3316), Expect = 0.0e+00
Identity = 635/635 (100.00%), Postives = 635/635 (100.00%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS
Sbjct: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY
Sbjct: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF
Sbjct: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 636
           YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK
Sbjct: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 635

BLAST of Moc01g33180 vs. ExPASy TrEMBL
Match: A0A6J1KW66 (alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496964 PE=3 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 566/629 (89.98%), Postives = 602/629 (95.71%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQR YFKVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLFTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP    P++L KV ENN+KGEF N+GGI+FRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNQLDKVRENNQKGEFKNNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSWVEKHLLDRI EKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTFENEGPKP VYLE A+NLADRLLSAFTSSPT IPL DVVL+DSSAHAAPGG+SSTS
Sbjct: 241 YGKTFENEGPKPAVYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMKSLPK+EGLVPIYISP SG+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGARE+NN+TYLHDMYEEAMKGV+HLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENNNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS++A+SPKMDHLVCFL GTLALGATKGISKEE+M KSLLSFEDLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEESMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVT+TGLAPEIVYF TE++SE   DGGNKN+KY++DIIIKP DRHNLLRPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+PKYRAWGW+IFEAFE+HTKVN+GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIE 630
           YLYLLFGD+ST+PL+KYVFNTEAHPLPIE
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIE 629

BLAST of Moc01g33180 vs. ExPASy TrEMBL
Match: A0A6J1ELX6 (alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111433756 PE=3 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 565/629 (89.83%), Postives = 601/629 (95.55%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQR YFKVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP    P+RL KVSENN+KGEF ++GGI+FRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNRLDKVSENNQKGEFKSNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSWVEKHLLDRI EKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTFENEGP P  YLE A+NLADRLLSAFTSSPT IPL DVVL+DSSAHAAPGG+SSTS
Sbjct: 241 YGKTFENEGPTPAAYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMKSLPK+EGLVPIYISP SG+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQKGARE++N+TYLHDMYEEAMKGV+HLLVRKSVPNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENHNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS++A+SPKMDHLVCFL GTLALGATKGISKEEAM KSLLSFEDLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEEAMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVT+TGLAPEIVYF TE++SE   DGGNKN+KY++DIIIKP DRHNLLRPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+PKYRAWGW+IFEAFE+HTKVN+GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIE 630
           YLYLLFGD+ST+PL+KYVFNTEAHPLPIE
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIE 629

BLAST of Moc01g33180 vs. ExPASy TrEMBL
Match: A0A1S3AZ69 (alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103484372 PE=3 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 562/635 (88.50%), Postives = 603/635 (94.96%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQR Y KVMTQNL TGS KRDYLSCSTGKFL+LL+IFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYLKVMTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLT+ TPVH  P++ AKVSENNEK E  N+GGI+FRNLWR+APRLPP+LPPDEKGSNTS
Sbjct: 61  LLLTNATPVH-VPNQPAKVSENNEKREIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQ GTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSW+EKHL+DRI EKGQVNLFETTIRV+GGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWIEKHLMDRIKEKGQVNLFETTIRVVGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTF NEGPKP VYLEIA+NLADRLLSAFTSSPT IPL DVVLKDSSAHAAPGG SSTS
Sbjct: 241 YGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISP++GEFSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQK    +NN TYLHDMYEEAMKGV+HLLVRKS+PNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS+ A+SPKMDHLVCFLSGTLALGATKGI+KEEAM+KSLLSF+DLENLNLAEDLAKTC+
Sbjct: 421 YGSDSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVTSTGLAPEI YF TE++SE+  DGGNKNSKY++DIIIKPLDRHNL+RPETVESLF
Sbjct: 481 EMYEVTSTGLAPEIAYFHTEEYSEQGHDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITGNPKYRAWGW+IFEAFE+HTKV+SGGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 636
           YLYLLFGDSST+PL+KYVFNTEAHPLP+EG  QI+
Sbjct: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQIQ 634

BLAST of Moc01g33180 vs. ExPASy TrEMBL
Match: A0A0A0KLY9 (alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G523340 PE=3 SV=1)

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 555/635 (87.40%), Postives = 602/635 (94.80%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQR Y KVMTQNL TGS KRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTS TPVH  P++LAKVSEN+EK E  N+GGI+FRNLWR+APRLPP+LPPDEKGSNTS
Sbjct: 61  LLLTSATPVH-VPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTS 120

Query: 121 YNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180
           Y+IDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQ GTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMG S+IVSEAGSW+EK+L+DRI EKGQVNLFETTIR++GGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGE 240

Query: 241 FGKTFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTS 300
           +GKTF NEGPKP VYLEIA+NLADRLLSAFTSSPT IPL DVVLKDSSAHAAPGG SSTS
Sbjct: 241 YGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRL 360
           EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISP++G+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQK    ++N TYLHDMYEEAMKGV+HLLVRKS+PNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELP 420

Query: 421 YGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCY 480
           YGS+  +SPKMDHLVCFLSGTLALGATKGI+KEEAM+KSLL+F+DLENLNLAEDLAKTC+
Sbjct: 421 YGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLF 540
           EMYEVTSTGLAPEI YF TE++SE+  DGGNK SKY++DIIIKPLDRHNL+RPETVESLF
Sbjct: 481 EMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLF 540

Query: 541 VLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+PKYRAWGW+IFEAFE+HTKV+SGGYTSLDDVTT+PPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTMPLNKYVFNTEAHPLPIEGISQIK 636
           YLYLLFGDSST+PL+KYVFNTEAHPLP++G  QI+
Sbjct: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Moc01g33180 vs. TAIR 10
Match: AT1G30000.1 (alpha-mannosidase 3 )

HSP 1 Score: 904.4 bits (2336), Expect = 5.0e-263
Identity = 451/632 (71.36%), Postives = 522/632 (82.59%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRPYFKVMTQNLRTGSAKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYSVKD+HYDNAKFR R   KV +Q+L T S KR+Y SCSTGKFL+L+L FG+A 
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSTTPVHSAPHRLAKVSENNEKGEFINSGGISFRNLWRRAPRLPPRLPPDE---KGS 120
           L+L S +P  S           NEKG+    GG+    L R+ PRLPPRL PDE   +GS
Sbjct: 61  LMLMSKSPNESG---------LNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGS 120

Query: 121 NTSYNIDPNLLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLG 180
           +T+ +   N   S+  W +RQQ VK+AF HAWSGY++YAMG+DELMP+SQ+G DGLGGLG
Sbjct: 121 STNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLG 180

Query: 181 ATVVDALDTAMIMGDSEIVSEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLR 240
           ATVVDALDTAMIMG   IVSEAGSWVE HLL+RI++KGQVNLFETTIRVLGGLLSAYHL 
Sbjct: 181 ATVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLS 240

Query: 241 AGEFGK-TFENEGPKPDVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGV 300
            GE G     + GPKP +YL IA++LADRLLSAFTSSPT +P  DV+L +S+AH APGG 
Sbjct: 241 GGEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGA 300

Query: 301 SSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGE 360
           SST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K+LPK EGLVPIYISP +G+F GE
Sbjct: 301 SSTAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGE 360

Query: 361 TIRLGSRGDSYYEYLLKVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFV 420
            IRLGSRGDSYYEYL+KVWLQ+GA+ ++N TYLHDMY EAMKGVRHLLV+ S+P GLVFV
Sbjct: 361 NIRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFV 420

Query: 421 GELPYGSNDALSPKMDHLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLA 480
           GELPYGS    SPKMDHLVCFL GTLALGATKG++KE+A+ ++LLSFEDLENL LAEDLA
Sbjct: 421 GELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLA 480

Query: 481 KTCYEMYEVTSTGLAPEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETV 540
           KTC+EMYEVT+TGLAPEI YF T+D++E+  DGGNK+S Y NDIIIKP DRHNLLRPETV
Sbjct: 481 KTCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETV 540

Query: 541 ESLFVLYRITGNPKYRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLG 600
           ESLFVLYRIT + KYR  GW+IFEAFEK+TKV SGGYTSLDDVT VPP RRDKMETFFLG
Sbjct: 541 ESLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLG 600

Query: 601 ETLKYLYLLFGDSSTMPLNKYVFNTEAHPLPI 629
           ETLKYLYLLFGD S +PL+K+VFNTEAHPLPI
Sbjct: 601 ETLKYLYLLFGDDSVIPLDKFVFNTEAHPLPI 620

BLAST of Moc01g33180 vs. TAIR 10
Match: AT1G51590.1 (alpha-mannosidase 1 )

HSP 1 Score: 359.4 bits (921), Expect = 6.0e-99
Identity = 224/496 (45.16%), Postives = 299/496 (60.28%), Query Frame = 0

Query: 137 RQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIV 196
           R+QKVK+A IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  E  
Sbjct: 97  RRQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQF 156

Query: 197 SEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGEFGKTFENEGPKPDVYL 256
            +A  WV   L    ++    ++FETTIRV+GGLLSAY L            G K  ++L
Sbjct: 157 QKAREWVASSL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFL 216

Query: 257 EIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHA---APGGVSSTSEVSTLQLEFNYLS 316
           E A+++ADRLL A+ ++PT IP + + L++ +AH    A GG S  ++  T QLEF  LS
Sbjct: 217 EKAKDIADRLLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALS 276

Query: 317 TVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRLGSRGDSYYEYLLK 376
             +GDPKY     KV+  +      +GL+PIYI+P +   S  T   G+ GDS+YEYLLK
Sbjct: 277 QRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLK 336

Query: 377 VWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMDH 436
           VW+Q    + + V    DM+E++MKG+   LV+KS P+   ++ E   G+N  L  KMD 
Sbjct: 337 VWVQ--GNKTSAVKPYRDMWEKSMKGLLS-LVKKSTPSSFTYICE-KNGNN--LIDKMDE 396

Query: 437 LVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTGLAPE 496
           L CF  G LALGA+ G   +          E+ + L+LA +LA TCY  Y+ T T LA E
Sbjct: 397 LACFAPGMLALGAS-GYGPD----------EEKKFLSLAGELAWTCYNFYQSTPTKLAGE 456

Query: 497 IVYFRT-EDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPKYR 556
             +F   +D S   S                     N+LRPETVESLF L+R+TGN  Y+
Sbjct: 457 NYFFTAGQDMSVGTS--------------------WNILRPETVESLFYLWRLTGNKTYQ 516

Query: 557 AWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTM 616
            WGW IF+AFEK+++V S GY  L DV T    + +KM++FFL ETLKYLYLLF  SS +
Sbjct: 517 EWGWNIFQAFEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVI 536

Query: 617 PLNKYVFNTEAHPLPI 629
            L+++VFNTEAHPL I
Sbjct: 577 SLDEWVFNTEAHPLKI 536

BLAST of Moc01g33180 vs. TAIR 10
Match: AT3G21160.1 (alpha-mannosidase 2 )

HSP 1 Score: 351.7 bits (901), Expect = 1.3e-96
Identity = 219/496 (44.15%), Postives = 294/496 (59.27%), Query Frame = 0

Query: 137 RQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIV 196
           R Q+VK+A +HAWS Y++YA G DEL P ++ G D  GGLGAT++DALDT  IMG  E  
Sbjct: 98  RMQRVKEAMVHAWSSYEKYAWGQDELQPQTKDGVDSFGGLGATMIDALDTLYIMGLDEQF 157

Query: 197 SEAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGEFGKTFENEGPKPDVYL 256
            +A  WV   L    ++    ++FETTIRV+GGLLSAY L            G K  ++L
Sbjct: 158 QKAREWVASSL--DFDKDYAASMFETTIRVVGGLLSAYDL-----------SGDK--IFL 217

Query: 257 EIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAH--AAPGGVSSTSEVSTLQLEFNYLST 316
           E A ++ADRLL A+  + + IP + + LK  +AH     GG S  ++  T QLEF  LS 
Sbjct: 218 EKAMDIADRLLPAW-DTQSGIPYNIINLKHGNAHNPTWAGGDSILADSGTEQLEFIALSQ 277

Query: 317 VSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRLGSRGDSYYEYLLKV 376
            +GDPKY     KV+  +      +GL+PIYI+P +   S  TI  G+ GDS+YEYLLKV
Sbjct: 278 RTGDPKYQQKVEKVISVLNKNFPADGLLPIYINPDTANPSQSTITFGAMGDSFYEYLLKV 337

Query: 377 WLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMDHL 436
           W+     + + V +  DM+E++M G+   LV+KS P    ++ E    S ++L  KMD L
Sbjct: 338 WV--FGNKTSAVKHYRDMWEKSMNGLLS-LVKKSTPLSFTYICE---KSGNSLIDKMDEL 397

Query: 437 VCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTGLAPEI 496
            CF  G LALGA+ G S + A  K  L+        LAE+LA TCY  Y+ T T LA E 
Sbjct: 398 ACFAPGMLALGAS-GYS-DPAEGKKFLT--------LAEELAWTCYNFYQSTPTKLAGEN 457

Query: 497 VYFRT-EDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPKYRA 556
            +F +  D S   S                     N+LRPETVESLF L+R+TGN  Y+ 
Sbjct: 458 YFFNSGSDMSVGTS--------------------WNILRPETVESLFYLWRLTGNKTYQE 517

Query: 557 WGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTMP 616
           WGW IFEAFEK++++ S GY  L DV T    + +KM++FFL ETLKYLYLLF  ++ +P
Sbjct: 518 WGWNIFEAFEKNSRIES-GYVGLKDVNT--GVKDNKMQSFFLAETLKYLYLLFSPTTVIP 538

Query: 617 LNKYVFNTEAHPLPIE 630
           L+++VFNTEAHPL I+
Sbjct: 578 LDEWVFNTEAHPLKIK 538

BLAST of Moc01g33180 vs. TAIR 10
Match: AT1G51590.2 (alpha-mannosidase 1 )

HSP 1 Score: 346.7 bits (888), Expect = 4.0e-95
Identity = 218/487 (44.76%), Postives = 291/487 (59.75%), Query Frame = 0

Query: 146 IHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIVSEAGSWVEK 205
           IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  E   +A  WV  
Sbjct: 2   IHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQFQKAREWVAS 61

Query: 206 HLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGEFGKTFENEGPKPDVYLEIARNLADR 265
            L    ++    ++FETTIRV+GGLLSAY L            G K  ++LE A+++ADR
Sbjct: 62  SL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFLEKAKDIADR 121

Query: 266 LLSAFTSSPTTIPLSDVVLKDSSAHA---APGGVSSTSEVSTLQLEFNYLSTVSGDPKYS 325
           LL A+ ++PT IP + + L++ +AH    A GG S  ++  T QLEF  LS  +GDPKY 
Sbjct: 122 LLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALSQRTGDPKYQ 181

Query: 326 IAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRLGSRGDSYYEYLLKVWLQKGARE 385
               KV+  +      +GL+PIYI+P +   S  T   G+ GDS+YEYLLKVW+Q    +
Sbjct: 182 QKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLKVWVQ--GNK 241

Query: 386 DNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMDHLVCFLSGTL 445
            + V    DM+E++MKG+   LV+KS P+   ++ E   G+N  L  KMD L CF  G L
Sbjct: 242 TSAVKPYRDMWEKSMKGLLS-LVKKSTPSSFTYICE-KNGNN--LIDKMDELACFAPGML 301

Query: 446 ALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFRT-ED 505
           ALGA+ G   +          E+ + L+LA +LA TCY  Y+ T T LA E  +F   +D
Sbjct: 302 ALGAS-GYGPD----------EEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTAGQD 361

Query: 506 FSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPKYRAWGWKIFEA 565
            S   S                     N+LRPETVESLF L+R+TGN  Y+ WGW IF+A
Sbjct: 362 MSVGTS--------------------WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQA 421

Query: 566 FEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTMPLNKYVFNT 625
           FEK+++V S GY  L DV T    + +KM++FFL ETLKYLYLLF  SS + L+++VFNT
Sbjct: 422 FEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVISLDEWVFNT 432

Query: 626 EAHPLPI 629
           EAHPL I
Sbjct: 482 EAHPLKI 432

BLAST of Moc01g33180 vs. TAIR 10
Match: AT5G43710.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-46
Identity = 157/504 (31.15%), Postives = 235/504 (46.63%), Query Frame = 0

Query: 138 QQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATVVDALDTAMIMGDSEIVS 197
           + +V+  F HA+ GY   A   DEL PLS QG D LGG   T++D+LDT  ++GD E  +
Sbjct: 41  RDEVRGMFYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTLIDSLDTLALLGDRERFT 100

Query: 198 EAGSWVEKHLLDRINEKGQVNLFETTIRVLGGLLSAYHLRAGEFG-----KTFENEGPKP 257
            +  W+ K+L   IN+   V++FETTIRVLGGLLSA HL A ++       ++ NE    
Sbjct: 101 SSVEWIGKNLQFNINK--TVSVFETTIRVLGGLLSA-HLIASDYATGMRIPSYNNE---- 160

Query: 258 DVYLEIARNLADRLLSAFTSSPTTIPLSDVVLKDSSAHAAPGGVSSTSEVSTLQLEFNYL 317
              L +A NLA R+L AF  +PT IP   V L           ++ST+   TL LEF  L
Sbjct: 161 --LLVLAENLARRMLPAF-DTPTGIPFGSVNLM-YGVDKHESKITSTAGGGTLSLEFGVL 220

Query: 318 STVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPYSGEFSGETIRLGSRGDSYYEYLL 377
           S ++ DP +   A   +  + +      LV  +I+ ++GE++ +   +G+  DS+YEYLL
Sbjct: 221 SRLTNDPVFEQVAKNAVRGLWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLL 280

Query: 378 KVWLQKGAREDNNVTYLHDMYEEAMKGVRHLLVRKSVPNGLVFVGELPYGSNDALSPKMD 437
           K ++  G  E     YL+ +++EA +     L +        +  E+   S   + P  +
Sbjct: 281 KAYILFGDEE-----YLY-IFQEAYRSAMQYLHKDP------WYVEVNMDSAAIVWPVFN 340

Query: 438 HLVCFLSGTLALGATKGISKEEAMNKSLLSFEDLENLNLAEDLAKTCYEMYEVTSTG--L 497
            L  F  G   L                    D++          + ++ Y  T  G  L
Sbjct: 341 SLQAFWPGLQVLAG------------------DVDPAIRTHTAFFSVWKRYGFTPEGFNL 400

Query: 498 APEIVYFRTEDFSEENSDGGNKNSKYVNDIIIKPLDRHNLLRPETVESLFVLYRITGNPK 557
           A   V +  + +                            LRPE +ES + LY+ T +P+
Sbjct: 401 ATLSVQYGQKSYP---------------------------LRPELIESTYWLYKATRDPR 460

Query: 558 YRAWGWKIFEAFEKHTKVNSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFG--- 617
           Y   G     + +   K    GY  + DV     ++ D ME+FFL ET+KYL+LLF    
Sbjct: 461 YLDAGRDFVASLQYGAKCPC-GYCHITDVEL--HKQEDHMESFFLAETVKYLWLLFDLAV 473

Query: 618 DSSTMPLN---KYVFNTEAHPLPI 629
           DS  +  N   KY+F+TE H LPI
Sbjct: 521 DSDNLVDNGPYKYIFSTEGHLLPI 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146514.10.0e+00100.00mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Momordica charantia][more]
XP_038882608.10.0e+0091.02mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X1 [Benincasa hispid... [more]
XP_023517941.10.0e+0089.98mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. ... [more]
XP_023003322.10.0e+0089.98mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita maxima] >XP_... [more]
XP_022926714.10.0e+0089.83mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita moschata] >X... [more]
Match NameE-valueIdentityDescription
Q93Y377.1e-26271.36Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana OX=3... [more]
Q8J0Q01.9e-11047.15Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans OX=5476 GN=MN... [more]
Q9UKM74.3e-11045.23Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sap... [more]
B2GUY01.2e-10743.33Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus n... [more]
A2AJ152.2e-10644.05Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musc... [more]
Match NameE-valueIdentityDescription
A0A6J1CYC20.0e+00100.00alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111015717 PE=3 SV=1[more]
A0A6J1KW660.0e+0089.98alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496964 PE=3 SV=1[more]
A0A6J1ELX60.0e+0089.83alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111433756 PE=3 SV=1[more]
A0A1S3AZ690.0e+0088.50alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103484372 PE=3 SV=1[more]
A0A0A0KLY90.0e+0087.40alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G523340 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30000.15.0e-26371.36alpha-mannosidase 3 [more]
AT1G51590.16.0e-9945.16alpha-mannosidase 1 [more]
AT3G21160.11.3e-9644.15alpha-mannosidase 2 [more]
AT1G51590.24.0e-9544.76alpha-mannosidase 1 [more]
AT5G43710.11.2e-4631.15Glycosyl hydrolase family 47 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001382Glycoside hydrolase family 47PRINTSPR00747GLYHDRLASE47coord: 269..288
score: 25.95
coord: 530..554
score: 52.0
coord: 177..191
score: 62.22
coord: 144..164
score: 51.25
coord: 430..446
score: 52.66
coord: 592..612
score: 57.37
coord: 216..234
score: 63.66
coord: 361..378
score: 58.2
IPR001382Glycoside hydrolase family 47PFAMPF01532Glyco_hydro_47coord: 144..628
e-value: 2.3E-158
score: 527.9
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 135..634
e-value: 8.2E-175
score: 584.2
NoneNo IPR availablePANTHERPTHR11742:SF88ALPHA-1,2-MANNOSIDASEcoord: 14..629
NoneNo IPR availablePANTHERPTHR11742MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDcoord: 14..629
IPR036026Seven-hairpin glycosidasesSUPERFAMILY48225Seven-hairpin glycosidasescoord: 134..628

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g33180.1Moc01g33180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006491 N-glycan processing
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity