Moc01g33070 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g33070
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	agamous-like MADS-box protein AGL61
Location	chr1: 23241220 .. 23241894 (+)
RNA-Seq Expression	Moc01g33070
Synteny	Moc01g33070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGCAAGAAGCAAACAAAGGGCCGACAAAAGATCGAAATGAAGAAGATAGAAAACGAGGACGATCGACTGATAACGTTCTCGAAGCGCAGGTCAGGCATCTACAAGAAGGCGAGCGAACTAGCAACACTATGCGGGGCTGAAGTCGGTGTCGTCGTATTCTCACCCGCGGGTAAGCCCTTCTCGTTTGCTCATCCCTCGATCGAATCCGTGGCCAACAAGTTCATCAACGAAACGCCGCAGCCCAACGACAACACTCACCCTCTCGTGGAAGCTCACAGGCGGGTGAGGATAAACGAGTTGAACCAACAACACAATCAACTTTTGAGCCAACTCGAGGCAGAGAAGGAAAAAGGGAAGGCACTAGAGAAAATGAAGAAGGTGAGAGGAAATAGTAATGGGCGTGGTTGGTGGGAGACTCCCACAGAGGAAATGGGGATGGAAGAGCTTCAACAAGTGGATGCTTCATTAGGAGATTTGTACACAAATGTATGCCATCAATTGAGAGAGAGAGGAGTCATTGGATCTTCTTCTTCTTCTTATTCTTCCATGCCCTTTAATGGAGCTGGTGAAGAAACTATTCCCTTTAACATTTTGGCAAATGGGCCTCCACCTCCTACTAATGCTTATCTCCCCCATGCCTTTGATTATGGACAAGATCAACTTCCATGA

mRNA sequence

ATGGAGGGCAAGAAGCAAACAAAGGGCCGACAAAAGATCGAAATGAAGAAGATAGAAAACGAGGACGATCGACTGATAACGTTCTCGAAGCGCAGGTCAGGCATCTACAAGAAGGCGAGCGAACTAGCAACACTATGCGGGGCTGAAGTCGGTGTCGTCGTATTCTCACCCGCGGGTAAGCCCTTCTCGTTTGCTCATCCCTCGATCGAATCCGTGGCCAACAAGTTCATCAACGAAACGCCGCAGCCCAACGACAACACTCACCCTCTCGTGGAAGCTCACAGGCGGGTGAGGATAAACGAGTTGAACCAACAACACAATCAACTTTTGAGCCAACTCGAGGCAGAGAAGGAAAAAGGGAAGGCACTAGAGAAAATGAAGAAGGTGAGAGGAAATAGTAATGGGCGTGGTTGGTGGGAGACTCCCACAGAGGAAATGGGGATGGAAGAGCTTCAACAAGTGGATGCTTCATTAGGAGATTTGTACACAAATGTATGCCATCAATTGAGAGAGAGAGGAGTCATTGGATCTTCTTCTTCTTCTTATTCTTCCATGCCCTTTAATGGAGCTGGTGAAGAAACTATTCCCTTTAACATTTTGGCAAATGGGCCTCCACCTCCTACTAATGCTTATCTCCCCCATGCCTTTGATTATGGACAAGATCAACTTCCATGA

Coding sequence (CDS)

ATGGAGGGCAAGAAGCAAACAAAGGGCCGACAAAAGATCGAAATGAAGAAGATAGAAAACGAGGACGATCGACTGATAACGTTCTCGAAGCGCAGGTCAGGCATCTACAAGAAGGCGAGCGAACTAGCAACACTATGCGGGGCTGAAGTCGGTGTCGTCGTATTCTCACCCGCGGGTAAGCCCTTCTCGTTTGCTCATCCCTCGATCGAATCCGTGGCCAACAAGTTCATCAACGAAACGCCGCAGCCCAACGACAACACTCACCCTCTCGTGGAAGCTCACAGGCGGGTGAGGATAAACGAGTTGAACCAACAACACAATCAACTTTTGAGCCAACTCGAGGCAGAGAAGGAAAAAGGGAAGGCACTAGAGAAAATGAAGAAGGTGAGAGGAAATAGTAATGGGCGTGGTTGGTGGGAGACTCCCACAGAGGAAATGGGGATGGAAGAGCTTCAACAAGTGGATGCTTCATTAGGAGATTTGTACACAAATGTATGCCATCAATTGAGAGAGAGAGGAGTCATTGGATCTTCTTCTTCTTCTTATTCTTCCATGCCCTTTAATGGAGCTGGTGAAGAAACTATTCCCTTTAACATTTTGGCAAATGGGCCTCCACCTCCTACTAATGCTTATCTCCCCCATGCCTTTGATTATGGACAAGATCAACTTCCATGA

Protein sequence

MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSSSYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP

Homology

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BLAST of Moc01g33070 vs. NCBI nr
Match: XP_022146674.1 (agamous-like MADS-box protein AGL61 [Momordica charantia])

HSP 1 Score: 446.8 bits (1148), Expect = 1.1e-121
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60
           MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK
Sbjct: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60

Query: 61  PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG 120
           PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG
Sbjct: 61  PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG 120

Query: 121 KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS 180
           KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS
Sbjct: 121 KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS 180

Query: 181 SYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP 225
           SYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP
Sbjct: 181 SYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP 224

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BLAST of Moc01g33070 vs. NCBI nr
Match: XP_022926768.1 (agamous-like MADS-box protein AGL61 [Cucurbita moschata])

HSP 1 Score: 346.3 bits (887), Expect = 2.0e-91
Identity = 184/230 (80.00%), Postives = 203/230 (88.26%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF
Sbjct: 52  KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 111

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
           AHP IESVANKF++E PQPNDNTHPLVEAHRR+RINELNQQHNQLLSQL+AEKEKGKALE
Sbjct: 112 AHPCIESVANKFLDENPQPNDNTHPLVEAHRRLRINELNQQHNQLLSQLDAEKEKGKALE 171

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS-SSYS 184
           K+KKVRGN NGRGWWE+PTEE+G+EELQQVD SLG+LY NVCH+L+ERGV+GSSS S+ +
Sbjct: 172 KLKKVRGN-NGRGWWESPTEELGIEELQQVDGSLGELYQNVCHELKERGVLGSSSYSASN 231

Query: 185 SMPFNGAG-EETIPFNILANGPP------PPTNAYL--PHAFDYGQDQLP 225
           ++ F+ AG EETIPFNILA G        P +  YL  P+ FDYGQ Q P
Sbjct: 232 TLGFDHAGREETIPFNILATGAAAAAAAGPSSATYLPDPNPFDYGQQQQP 280

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BLAST of Moc01g33070 vs. NCBI nr
Match: KAG6594479.1 (Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026476.1 Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 344.7 bits (883), Expect = 5.8e-91
Identity = 183/228 (80.26%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF
Sbjct: 9   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 68

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
           AHP IESVANKF++E PQPNDNTHPLVEAHRR+RINELNQQHNQLLSQL+AEKEKGKALE
Sbjct: 69  AHPCIESVANKFLDENPQPNDNTHPLVEAHRRLRINELNQQHNQLLSQLDAEKEKGKALE 128

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS-SSYS 184
           K+KKVRGN NGRGWWE+PTEE+G+EELQQVD SLG+LY NVCH+L+ERGV+GSSS S+ +
Sbjct: 129 KLKKVRGN-NGRGWWESPTEELGIEELQQVDGSLGELYQNVCHELKERGVLGSSSYSASN 188

Query: 185 SMPFNGAG-EETIPFNILANGPP----PPTNAYL--PHAFDYGQDQLP 225
           ++ F+ AG EETIPFNILA G      P +  YL  P+ FDYGQ   P
Sbjct: 189 TLGFDHAGREETIPFNILATGAAAAAGPSSATYLPDPNPFDYGQQPQP 235

[Close]

BLAST of Moc01g33070 vs. NCBI nr
Match: XP_023518350.1 (agamous-like MADS-box protein AGL62 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343.2 bits (879), Expect = 1.7e-90
Identity = 182/229 (79.48%), Postives = 202/229 (88.21%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF
Sbjct: 9   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 68

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
           AHP IESVANKF++E PQPNDNTHPLVEAHRR+RINELNQQHNQLLSQL+AEKEKGKALE
Sbjct: 69  AHPCIESVANKFLDENPQPNDNTHPLVEAHRRLRINELNQQHNQLLSQLDAEKEKGKALE 128

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS-SSYS 184
           K+KKVRGN NGRGWWE+PTEE+G++ELQQVD SLG+LY NVCH+L+ERGV+GSSS S+ +
Sbjct: 129 KLKKVRGN-NGRGWWESPTEELGIDELQQVDGSLGELYQNVCHELKERGVLGSSSYSASN 188

Query: 185 SMPFNGAG-EETIPFNILANGPP-----PPTNAYL--PHAFDYGQDQLP 225
           ++ F+ AG EETIPFNILA G       P +  YL  P+ FDYGQ   P
Sbjct: 189 TLGFDHAGREETIPFNILATGAAAAAAGPSSATYLPDPNPFDYGQQPQP 236

[Close]

BLAST of Moc01g33070 vs. NCBI nr
Match: XP_023003356.1 (agamous-like MADS-box protein AGL62 [Cucurbita maxima])

HSP 1 Score: 341.7 bits (875), Expect = 4.9e-90
Identity = 182/230 (79.13%), Postives = 201/230 (87.39%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF
Sbjct: 9   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 68

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
           AHP IESVANKF++E PQPNDNTHPLVEAHRR+RINELNQQHNQLLSQL+AEKEKGKALE
Sbjct: 69  AHPCIESVANKFLDENPQPNDNTHPLVEAHRRLRINELNQQHNQLLSQLDAEKEKGKALE 128

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS-SSYS 184
           K+KKVRGN NGRGWWE+PTEE+ +EELQQVD SLG+LY NVCH+L+ERGV+GSSS S+ +
Sbjct: 129 KLKKVRGN-NGRGWWESPTEELAIEELQQVDGSLGELYQNVCHELKERGVLGSSSYSASN 188

Query: 185 SMPFNGAG-EETIPFNILANGPP------PPTNAYL--PHAFDYGQDQLP 225
           ++ F+ AG EETIPFNILA G        P +  YL  P+ FDYGQ   P
Sbjct: 189 TLGFDHAGREETIPFNILATGAAAASAAGPSSATYLPDPNPFDYGQQPQP 237

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BLAST of Moc01g33070 vs. ExPASy Swiss-Prot
Match: Q9FKK2 (Agamous-like MADS-box protein AGL62 OS=Arabidopsis thaliana OX=3702 GN=AGL62 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.4e-33
Identity = 79/160 (49.38%), Postives = 108/160 (67.50%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           K++KGRQKIEM K++NE +  +TFSKRRSG++KKASEL TLCGAEV +VVFSP  K FSF
Sbjct: 3   KKSKGRQKIEMVKMKNESNLQVTFSKRRSGLFKKASELCTLCGAEVAIVVFSPGRKVFSF 62

Query: 65  AHPSIESVANKFINETPQP--NDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKA 124
            HP+++SV ++FIN  P P    N   L E  R   + +LN    Q+LSQLE EK+K   
Sbjct: 63  GHPNVDSVIDRFINNNPLPPHQHNNMQLRETRRNSIVQDLNNHLTQVLSQLETEKKK--- 122

Query: 125 LEKMKKVRGNSNGRG-WWETPTEEMGMEELQQVDASLGDL 162
            +++KK+R  +   G WWE P EE+ + +L+    +L +L
Sbjct: 123 YDELKKIREKTKALGNWWEDPVEELALSQLEGFKGNLENL 159

[Close]

BLAST of Moc01g33070 vs. ExPASy Swiss-Prot
Match: Q4PSU4 (Agamous-like MADS-box protein AGL61 OS=Arabidopsis thaliana OX=3702 GN=AGL61 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.4e-33
Identity = 86/211 (40.76%), Postives = 125/211 (59.24%), Query Frame = 0


Query: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60
           M  KK++ GRQKI M KI+ E  R +TFSKRR+G++KKASEL TLCGAE+G++VFSPA K
Sbjct: 55  MSKKKESIGRQKIPMVKIKKESHRQVTFSKRRAGLFKKASELCTLCGAEIGIIVFSPAKK 114

Query: 61  PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG 120
           PFSF HPS+ESV +++++        +  L  +       ELN Q   +LS++E EK+KG
Sbjct: 115 PFSFGHPSVESVLDRYVSRNNMSLAQSQQLQGS--PAASCELNMQLTHILSEVEEEKKKG 174

Query: 121 KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS 180
           +A+E+M+K     +   WWE P EEM M +LQ++  +L         +LR+  V   +S 
Sbjct: 175 QAMEEMRKESVRRSMINWWEKPVEEMNMVQLQEMKYAL--------EELRKTVVTNMASF 234

Query: 181 SYSSMPFNGAGEETIPFNILANGPPPPTNAY 212
           + +     G  +  +      N P  P+N Y
Sbjct: 235 NEAKDDVFGFLDNKVTVPPYVNMPSGPSNIY 255

[Close]

BLAST of Moc01g33070 vs. ExPASy Swiss-Prot
Match: O64703 (Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.9e-21
Identity = 60/146 (41.10%), Postives = 100/146 (68.49%), Query Frame = 0


Query: 9   GRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSFAHPS 68
           GR+KI+M+ +++ + R +TFSKRR+G++KKASELATLC AE+G+VVFSP GKPFS+  P+
Sbjct: 2   GRRKIKMEMVQDMNTRQVTFSKRRTGLFKKASELATLCNAELGIVVFSPGGKPFSYGKPN 61

Query: 69  IESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG-KALEKMK 128
           ++SVA +F+ E    +D+       + R ++  L+++ + L  ++EAEKE+G K+ EK++
Sbjct: 62  LDSVAERFMREY-DDSDSGDEEKSGNYRPKLKRLSERLDLLNQEVEAEKERGEKSQEKLE 121

Query: 129 KVRGNSNGRGWWETPTEEMGMEELQQ 154
                S G   ++   E + ++EL +
Sbjct: 122 -----SAGDERFKESIETLTLDELNE 141

[Close]

BLAST of Moc01g33070 vs. ExPASy Swiss-Prot
Match: Q9LMM8 (Agamous-like MADS-box protein AGL28 OS=Arabidopsis thaliana OX=3702 GN=AGL28 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.6e-21
Identity = 71/221 (32.13%), Postives = 119/221 (53.85%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           ++  GR+KIE+ K+ NE +  +TFSKRRSG++KK SEL TLC AE+ ++VFSP+GK +SF
Sbjct: 3   RKNLGRRKIELVKMTNESNLQVTFSKRRSGLFKKGSELCTLCDAEIAIIVFSPSGKAYSF 62

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
            HP++  + +  +    + N NT+   E+  ++RI  LN+   +++++ E E+E  +++ 
Sbjct: 63  GHPNVNKLLDHSLGRVIRHN-NTN-FAESRTKLRIQMLNESLTEVMAEKEKEQETKQSI- 122

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNV---CHQLRERGV---IGSS 184
            ++  R N +   WW     E+ + +   +   L  L   V     QL  R +   +GSS
Sbjct: 123 -VQNERENKDAEKWWRNSPTELNLAQSTSMKCDLEALKKEVDEKVAQLHHRNLNFYVGSS 182

Query: 185 SSSYSSMPFNGAGEETIP--FNILANGPPPPTNAYLPHAFD 218
           S+  +    +G    T    F+   N    PT   LP  F+
Sbjct: 183 SNVAAPAAVSGGNISTNHGFFDQNGNSTSAPT---LPFGFN 216

[Close]

BLAST of Moc01g33070 vs. ExPASy Swiss-Prot
Match: O80807 (Agamous-like MADS-box protein AGL23 OS=Arabidopsis thaliana OX=3702 GN=AGL23 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.5e-20
Identity = 61/197 (30.96%), Postives = 110/197 (55.84%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           K+T GR+K+E+ K+  E +  +TFSKR++G++KKASE  TLC A++ ++VFSPAGK FSF
Sbjct: 3   KKTLGRRKVEIVKMTKESNLQVTFSKRKAGLFKKASEFCTLCDAKIAMIVFSPAGKVFSF 62

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
            HP+++ + + F       ++NT+ L E++ ++ +  LN+ + ++  + E EKE+     
Sbjct: 63  GHPNVDVLLDHF-RGCVVGHNNTN-LDESYTKLHVQMLNKSYTEV--KAEVEKEQKNKQS 122

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEE-------LQQVDASLGDLYTNVCHQLRERGVIGS 184
           + +  R N N   WW     E+ + +       L+ +   + +    + HQ      +GS
Sbjct: 123 RAQNERENENAEEWWSKSPLELNLSQSTCMIRVLKDLKKIVDEKAIQLIHQTNPNFYVGS 182

Query: 185 SSSSYSSMPFNGAGEET 195
           SS++ +    +G    T
Sbjct: 183 SSNAAAPATVSGGNIST 195

[Close]

BLAST of Moc01g33070 vs. ExPASy TrEMBL
Match: A0A6J1CYR8 (agamous-like MADS-box protein AGL61 OS=Momordica charantia OX=3673 GN=LOC111015822 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.3e-122
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60
           MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK
Sbjct: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60

Query: 61  PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG 120
           PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG
Sbjct: 61  PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG 120

Query: 121 KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS 180
           KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS
Sbjct: 121 KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS 180

Query: 181 SYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP 225
           SYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP
Sbjct: 181 SYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYGQDQLP 224

[Close]

BLAST of Moc01g33070 vs. ExPASy TrEMBL
Match: A0A6J1EJ54 (agamous-like MADS-box protein AGL61 OS=Cucurbita moschata OX=3662 GN=LOC111433790 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.7e-92
Identity = 184/230 (80.00%), Postives = 203/230 (88.26%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF
Sbjct: 52  KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 111

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
           AHP IESVANKF++E PQPNDNTHPLVEAHRR+RINELNQQHNQLLSQL+AEKEKGKALE
Sbjct: 112 AHPCIESVANKFLDENPQPNDNTHPLVEAHRRLRINELNQQHNQLLSQLDAEKEKGKALE 171

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS-SSYS 184
           K+KKVRGN NGRGWWE+PTEE+G+EELQQVD SLG+LY NVCH+L+ERGV+GSSS S+ +
Sbjct: 172 KLKKVRGN-NGRGWWESPTEELGIEELQQVDGSLGELYQNVCHELKERGVLGSSSYSASN 231

Query: 185 SMPFNGAG-EETIPFNILANGPP------PPTNAYL--PHAFDYGQDQLP 225
           ++ F+ AG EETIPFNILA G        P +  YL  P+ FDYGQ Q P
Sbjct: 232 TLGFDHAGREETIPFNILATGAAAAAAAGPSSATYLPDPNPFDYGQQQQP 280

[Close]

BLAST of Moc01g33070 vs. ExPASy TrEMBL
Match: A0A6J1KP08 (agamous-like MADS-box protein AGL62 OS=Cucurbita maxima OX=3661 GN=LOC111496989 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.4e-90
Identity = 182/230 (79.13%), Postives = 201/230 (87.39%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF
Sbjct: 9   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 68

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
           AHP IESVANKF++E PQPNDNTHPLVEAHRR+RINELNQQHNQLLSQL+AEKEKGKALE
Sbjct: 69  AHPCIESVANKFLDENPQPNDNTHPLVEAHRRLRINELNQQHNQLLSQLDAEKEKGKALE 128

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS-SSYS 184
           K+KKVRGN NGRGWWE+PTEE+ +EELQQVD SLG+LY NVCH+L+ERGV+GSSS S+ +
Sbjct: 129 KLKKVRGN-NGRGWWESPTEELAIEELQQVDGSLGELYQNVCHELKERGVLGSSSYSASN 188

Query: 185 SMPFNGAG-EETIPFNILANGPP------PPTNAYL--PHAFDYGQDQLP 225
           ++ F+ AG EETIPFNILA G        P +  YL  P+ FDYGQ   P
Sbjct: 189 TLGFDHAGREETIPFNILATGAAAASAAGPSSATYLPDPNPFDYGQQPQP 237

[Close]

BLAST of Moc01g33070 vs. ExPASy TrEMBL
Match: A0A0A0KNT7 (MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G522745 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.6e-78
Identity = 171/266 (64.29%), Postives = 195/266 (73.31%), Query Frame = 0


Query: 4   KKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFS 63
           KKQTKGRQKIEMKKI NEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFS
Sbjct: 14  KKQTKGRQKIEMKKIVNEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFS 73

Query: 64  FAHPSIESVANKFIN----------------ETPQPNDN------------THPLVEAHR 123
           FAHP IE++ANKF+N                ++   N+N             HPLVEAHR
Sbjct: 74  FAHPCIETIANKFLNGNKNNKGNNDDNNNNGDSSSSNNNNNNSNNNNNSNAAHPLVEAHR 133

Query: 124 RVRINELNQQHNQLLSQLEAEKEKGKALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVD 183
           RVRINELNQQHNQLLSQL+AEKEKGKALEK+K+VRG  NGRGWWETPTEE+G+EELQ+VD
Sbjct: 134 RVRINELNQQHNQLLSQLDAEKEKGKALEKLKRVRG--NGRGWWETPTEELGIEELQEVD 193

Query: 184 ASLGDLYTNVCHQLRERGVIG-------SSSSSYSSMPF----NGAGEETIPFNILANG- 223
           AS G++Y+NVCHQL++RGVIG       +++ + SSM F    +  GEETIPFNILA   
Sbjct: 194 ASFGEIYSNVCHQLKQRGVIGCCSYNNYNNNMNNSSMGFPLTNDQLGEETIPFNILAGNV 253

[Close]

BLAST of Moc01g33070 vs. ExPASy TrEMBL
Match: A0A7N2R569 (MADS-box domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.2e-67
Identity = 141/221 (63.80%), Postives = 174/221 (78.73%), Query Frame = 0


Query: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60
           MEGKK TKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASEL TLCGAEVGVV+FSPAGK
Sbjct: 1   MEGKK-TKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELVTLCGAEVGVVIFSPAGK 60

Query: 61  PFSFAHPSIESVANKFINETPQP--NDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKE 120
           PF+F HPS+ESVAN+F+ + P P   DNTHPLVEAHRRVRINELNQQ+N+LLS LEAEK+
Sbjct: 61  PFTFGHPSVESVANRFLRQNPPPGQGDNTHPLVEAHRRVRINELNQQYNELLSLLEAEKD 120

Query: 121 KGKALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSS 180
           +GKAL+K+ K RGN   +GWWE PTEE+G +EL+Q++A L +L+ N+C+ ++E+   G +
Sbjct: 121 QGKALQKLTKARGN---KGWWEVPTEELGYQELKQMNAQLEELHKNLCNHMKEKAFAGGA 180

Query: 181 SSSYSSMPFNGAGEETIPFNILANGPPPPTNAYLPHAFDYG 220
             S S+     + + +IPF   A+   PP    LP ++ YG
Sbjct: 181 LPSSSTYFPGNSSQGSIPFIPNASDADPP----LPPSYGYG 213

[Close]

BLAST of Moc01g33070 vs. TAIR 10
Match: AT5G60440.1 (AGAMOUS-like 62 )

HSP 1 Score: 143.3 bits (360), Expect = 2.4e-34
Identity = 79/160 (49.38%), Postives = 108/160 (67.50%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           K++KGRQKIEM K++NE +  +TFSKRRSG++KKASEL TLCGAEV +VVFSP  K FSF
Sbjct: 3   KKSKGRQKIEMVKMKNESNLQVTFSKRRSGLFKKASELCTLCGAEVAIVVFSPGRKVFSF 62

Query: 65  AHPSIESVANKFINETPQP--NDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKA 124
            HP+++SV ++FIN  P P    N   L E  R   + +LN    Q+LSQLE EK+K   
Sbjct: 63  GHPNVDSVIDRFINNNPLPPHQHNNMQLRETRRNSIVQDLNNHLTQVLSQLETEKKK--- 122

Query: 125 LEKMKKVRGNSNGRG-WWETPTEEMGMEELQQVDASLGDL 162
            +++KK+R  +   G WWE P EE+ + +L+    +L +L
Sbjct: 123 YDELKKIREKTKALGNWWEDPVEELALSQLEGFKGNLENL 159

[Close]

BLAST of Moc01g33070 vs. TAIR 10
Match: AT2G24840.1 (AGAMOUS-like 61 )

HSP 1 Score: 142.9 bits (359), Expect = 3.1e-34
Identity = 86/211 (40.76%), Postives = 125/211 (59.24%), Query Frame = 0


Query: 1   MEGKKQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGK 60
           M  KK++ GRQKI M KI+ E  R +TFSKRR+G++KKASEL TLCGAE+G++VFSPA K
Sbjct: 55  MSKKKESIGRQKIPMVKIKKESHRQVTFSKRRAGLFKKASELCTLCGAEIGIIVFSPAKK 114

Query: 61  PFSFAHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKG 120
           PFSF HPS+ESV +++++        +  L  +       ELN Q   +LS++E EK+KG
Sbjct: 115 PFSFGHPSVESVLDRYVSRNNMSLAQSQQLQGS--PAASCELNMQLTHILSEVEEEKKKG 174

Query: 121 KALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSSS 180
           +A+E+M+K     +   WWE P EEM M +LQ++  +L         +LR+  V   +S 
Sbjct: 175 QAMEEMRKESVRRSMINWWEKPVEEMNMVQLQEMKYAL--------EELRKTVVTNMASF 234

Query: 181 SYSSMPFNGAGEETIPFNILANGPPPPTNAY 212
           + +     G  +  +      N P  P+N Y
Sbjct: 235 NEAKDDVFGFLDNKVTVPPYVNMPSGPSNIY 255

[Close]

BLAST of Moc01g33070 vs. TAIR 10
Match: AT3G04100.1 (AGAMOUS-like 57 )

HSP 1 Score: 126.3 bits (316), Expect = 3.0e-29
Identity = 68/168 (40.48%), Postives = 111/168 (66.07%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           ++TKG+QKIEMKK+EN  DR+ITFSKR++GI+KK +EL  +C  EV  ++FS   KP++F
Sbjct: 10  RKTKGKQKIEMKKVENYGDRMITFSKRKTGIFKKMNELVAMCDVEVAFLIFSQPKKPYTF 69

Query: 65  AHPSIESVANKFIN---ETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGK 124
           AHPS++ VA++  N   + P   D+T PLVEA+++ R+++L ++   L  +L  + EK K
Sbjct: 70  AHPSMKKVADRLKNPSRQEPLERDDTRPLVEAYKKRRLHDLVKKMEALEEELAMDLEKLK 129

Query: 125 ALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQL 170
            L++ +  +     + WW  P+E +  +ELQQ   ++ +L  N+C  +
Sbjct: 130 LLKESRNEK--KLDKMWWNFPSEGLSAKELQQRYQAMLELRDNLCDNM 175

[Close]

BLAST of Moc01g33070 vs. TAIR 10
Match: AT4G36590.1 (MADS-box transcription factor family protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.1e-26
Identity = 69/183 (37.70%), Postives = 106/183 (57.92%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           + TKGRQKIEMKK+ENE +  +TFSKRR G++KKASEL TL GAE+ ++VFSP GK FSF
Sbjct: 3   RSTKGRQKIEMKKMENESNLQVTFSKRRFGLFKKASELCTLSGAEILLIVFSPGGKVFSF 62

Query: 65  AHPSIESVANKFINETPQP-----NDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEK 124
            HPS++ + ++F N           +N   LVE      I  LN    ++L+  E EK+K
Sbjct: 63  GHPSVQELIHRFSNPNHNSAIVHHQNNNLQLVETRPDRNIQYLNNILTEVLANQEKEKQK 122

Query: 125 GKALEKMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRERGVIGSSS 183
              L+ +K+ R       W+E   +++ M E  Q+ ++L D+   +  ++ +   +  S 
Sbjct: 123 RMVLDLLKESREQVG--NWYEKDVKDLDMNETNQLISALQDVKKKLVREMSQYSQVNVSQ 182

[Close]

BLAST of Moc01g33070 vs. TAIR 10
Match: AT1G28460.1 (AGAMOUS-like 59 )

HSP 1 Score: 113.2 bits (282), Expect = 2.7e-25
Identity = 59/168 (35.12%), Postives = 103/168 (61.31%), Query Frame = 0


Query: 5   KQTKGRQKIEMKKIENEDDRLITFSKRRSGIYKKASELATLCGAEVGVVVFSPAGKPFSF 64
           K+TKG+QKI +KKIE ++ R +TFSKR +GIY K SEL+ LCG EV  + +S +GKP++F
Sbjct: 4   KKTKGKQKINIKKIEKDEGRSVTFSKRLNGIYTKISELSILCGVEVAFIGYSCSGKPYTF 63

Query: 65  AHPSIESVANKFINETPQPNDNTHPLVEAHRRVRINELNQQHNQLLSQLEAEKEKGKALE 124
             PS ++VA +F+N     + ++  ++ AH++ +I EL +++N+L+ +L+ ++ K K   
Sbjct: 64  GSPSFQAVAERFLNGDASSSSSSSLVMNAHKQAKIQELCKKYNRLVEELKVDEVKVKKAA 123

Query: 125 KMKKVRGNSNGRGWWETPTEEMGMEELQQVDASLGDLYTNVCHQLRER 173
            + + R  +    W   P +    E+ +++     +LY  +C Q   R
Sbjct: 124 ALAETRVVNKDVWWKVDPNDVKDHEKAKKMMEKYQELYDKLCEQAASR 171

The following BLAST results are available for this feature:

BLAST of Moc01g33070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146674.1	1.1e-121	100.00	agamous-like MADS-box protein AGL61 [Momordica charantia]	[more]
XP_022926768.1	2.0e-91	80.00	agamous-like MADS-box protein AGL61 [Cucurbita moschata]	[more]
KAG6594479.1	5.8e-91	80.26	Agamous-like MADS-box protein AGL62, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023518350.1	1.7e-90	79.48	agamous-like MADS-box protein AGL62 [Cucurbita pepo subsp. pepo]	[more]
XP_023003356.1	4.9e-90	79.13	agamous-like MADS-box protein AGL62 [Cucurbita maxima]	[more]

BLAST of Moc01g33070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKK2	3.4e-33	49.38	Agamous-like MADS-box protein AGL62 OS=Arabidopsis thaliana OX=3702 GN=AGL62 PE=...	[more]
Q4PSU4	4.4e-33	40.76	Agamous-like MADS-box protein AGL61 OS=Arabidopsis thaliana OX=3702 GN=AGL61 PE=...	[more]
O64703	3.9e-21	41.10	Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=...	[more]
Q9LMM8	6.6e-21	32.13	Agamous-like MADS-box protein AGL28 OS=Arabidopsis thaliana OX=3702 GN=AGL28 PE=...	[more]
O80807	2.5e-20	30.96	Agamous-like MADS-box protein AGL23 OS=Arabidopsis thaliana OX=3702 GN=AGL23 PE=...	[more]

BLAST of Moc01g33070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CYR8	5.3e-122	100.00	agamous-like MADS-box protein AGL61 OS=Momordica charantia OX=3673 GN=LOC1110158...	[more]
A0A6J1EJ54	9.7e-92	80.00	agamous-like MADS-box protein AGL61 OS=Cucurbita moschata OX=3662 GN=LOC11143379...	[more]
A0A6J1KP08	2.4e-90	79.13	agamous-like MADS-box protein AGL62 OS=Cucurbita maxima OX=3661 GN=LOC111496989 ...	[more]
A0A0A0KNT7	1.6e-78	64.29	MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G522745 PE...	[more]
A0A7N2R569	2.2e-67	63.80	MADS-box domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of Moc01g33070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60440.1	2.4e-34	49.38	AGAMOUS-like 62	[more]
AT2G24840.1	3.1e-34	40.76	AGAMOUS-like 61	[more]
AT3G04100.1	3.0e-29	40.48	AGAMOUS-like 57	[more]
AT4G36590.1	4.1e-26	37.70	MADS-box transcription factor family protein	[more]
AT1G28460.1	2.7e-25	35.12	AGAMOUS-like 59	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 1..21
None	No IPR available	COILS	Coil	Coil	coord: 96..126
None	No IPR available	GENE3D	6.10.140.920		coord: 88..170 e-value: 1.4E-7 score: 33.7
None	No IPR available	PANTHER	PTHR11945:SF648	BOX PROTEIN, PUTATIVE-RELATED	coord: 7..185
None	No IPR available	PANTHER	PTHR11945	MADS BOX PROTEIN	coord: 7..185
IPR002100	Transcription factor, MADS-box	PRINTS	PR00404	MADSDOMAIN	coord: 45..66 score: 43.8 coord: 10..30 score: 51.0 coord: 30..45 score: 61.67
IPR002100	Transcription factor, MADS-box	SMART	SM00432	madsneu2	coord: 8..67 e-value: 1.2E-30 score: 117.8
IPR002100	Transcription factor, MADS-box	PFAM	PF00319	SRF-TF	coord: 17..64 e-value: 1.1E-23 score: 82.5
IPR002100	Transcription factor, MADS-box	PROSITE	PS50066	MADS_BOX_2	coord: 8..68 score: 25.819454
IPR036879	Transcription factor, MADS-box superfamily	GENE3D	3.40.1810.10		coord: 20..87 e-value: 3.3E-24 score: 86.5
IPR036879	Transcription factor, MADS-box superfamily	SUPERFAMILY	55455	SRF-like	coord: 9..84
IPR033896	MADS MEF2-like	CDD	cd00265	MADS_MEF2_like	coord: 9..78 e-value: 2.50849E-35 score: 118.424

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g33070.1	Moc01g33070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045944	positive regulation of transcription by RNA polymerase II
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0000977	RNA polymerase II transcription regulatory region sequence-specific DNA binding