Moc01g30490 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g30490
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncytochrome b561 and DOMON domain-containing protein At5g35735-like
Locationchr1: 21504316 .. 21505801 (-)
RNA-Seq ExpressionMoc01g30490
SyntenyMoc01g30490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAGGTTCCCACTGCTCCCTACTTCTCAGCCTAATTTTTTCTGTACTGTGTTCAACGGTTTACTCGACCCCTTGCAATTCTTATGGCCAAGATCACGAGTACTGTAATAACTCTCTAATTACCAAAATTGGGAGGGACAACAAAATTGAAGATACGGCGGAAGCCGCGGCCGCGCACCAGGCAAACTCCACCTCCGGCCTCCACAGCCCAGATACGAAAGTAACATCATCAACTCCAAAAATATGGAAGAGCCAGACCAGCTTGCCTTACACCACTCTCTTAAGACACGTAAGAAAACTATTTTGAATTGTGCGCCGTGTATATGCAATGCAGCTAAAATAATGATACTGAAGATTTTTGGGAGATGTTGGCACAAACAGGTTCATGGAGTTCTGAATATTATAGGATGGGGAACTTTGATGCCCAGTGGGATTATGATCGCCAGATACTTGAGAGAATTCCCCATAAGCTGCGAGCGATGGTATTCGGCTCACGCTGTGTGCCAAACTGCTGGGTATATCATGGGCATCGTCGGATGGGGCTTCGGAATCTCCGCCGCTAACTCCTCCGAACGCAGCTCTCGCCTACCATTCTTAGCCCTCGGCATTACCATCATCGTCCTTGCAACCATTCAGGTAAGTTTCTCTGCAAATTTAAGTTTTGTGATCTCTAAATAAATTTTAGACCTTTTCCCTTCTGTTTTAAGCATAATTTTTAAATATAAAAAGTTAAAAATGAAATGCTTATTAAATGGTATGACGTAGATTTTAAATTTTAATTTTGTGTGTAATAAAATCTTGTCGAATTTAACAATTAATGACCATGTTAACCCATCAAATAATTTTCAGAATCCCTTTTGGACTCTGATACCATATCAGATTTTATTTCAAGAACAATCGACAATGAGAAAAATGATTGATAACTTCTCAACGTGGAATCCGTAACAGTTATTCATTATTATAAGTTAGTACGGTCTCTTTAGATATTTTTGAACTTTCAACTTTGTGTTTTGAATTAAATTTTGTGTATAATATGTATTTGATGTTTGAATTTTGTATCTAGTAAATCAAAATTTTTATCAGAATATGGAGTCGTAAAAGTTGATGGATGTTTTGAAGGACACGAGGATAAGTTAAAGAAATCCAAATTAAATGTGTGTAACTCAACCAAAGTTTGATGTTTCAATTTTGTGCAGCTGCTTGCTATATGTGTACAGCCGAAGAAGGAGAGCGGGTGGCGGCGGAGGTGGGAAATGTACCACCATGTTATGGGATACGTGATAATGGGACTGATAATAGGCGACATATTCGAAGGTATTAATACGCAGCGCCACCCAGAGAAATGGCGGTGGACTTACGTGGGAATTGTTTCTTTTCTTGCACTTGTGGCTGCGGCTTTGGAGATTCACAAACGAATCAAGCTTAAACTCTTCAAACAGGCTATGAAACTCAATGCCAATATGCATTCTCCCACTACTTGA

mRNA sequence

ATGGCTTCAGGTTCCCACTGCTCCCTACTTCTCAGCCTAATTTTTTCTGTACTGTGTTCAACGGTTTACTCGACCCCTTGCAATTCTTATGGCCAAGATCACGAGTACTGTAATAACTCTCTAATTACCAAAATTGGGAGGGACAACAAAATTGAAGATACGGCGGAAGCCGCGGCCGCGCACCAGGCAAACTCCACCTCCGGCCTCCACAGCCCAGATACGAAAGTAACATCATCAACTCCAAAAATATGGAAGAGCCAGACCAGCTTGCCTTACACCACTCTCTTAAGACACGTTCATGGAGTTCTGAATATTATAGGATGGGGAACTTTGATGCCCAGTGGGATTATGATCGCCAGATACTTGAGAGAATTCCCCATAAGCTGCGAGCGATGGTATTCGGCTCACGCTGTGTGCCAAACTGCTGGGTATATCATGGGCATCGTCGGATGGGGCTTCGGAATCTCCGCCGCTAACTCCTCCGAACGCAGCTCTCGCCTACCATTCTTAGCCCTCGGCATTACCATCATCGTCCTTGCAACCATTCAGCTGCTTGCTATATGTGTACAGCCGAAGAAGGAGAGCGGGTGGCGGCGGAGGTGGGAAATGTACCACCATGTTATGGGATACGTGATAATGGGACTGATAATAGGCGACATATTCGAAGGTATTAATACGCAGCGCCACCCAGAGAAATGGCGGTGGACTTACGTGGGAATTGTTTCTTTTCTTGCACTTGTGGCTGCGGCTTTGGAGATTCACAAACGAATCAAGCTTAAACTCTTCAAACAGGCTATGAAACTCAATGCCAATATGCATTCTCCCACTACTTGA

Coding sequence (CDS)

ATGGCTTCAGGTTCCCACTGCTCCCTACTTCTCAGCCTAATTTTTTCTGTACTGTGTTCAACGGTTTACTCGACCCCTTGCAATTCTTATGGCCAAGATCACGAGTACTGTAATAACTCTCTAATTACCAAAATTGGGAGGGACAACAAAATTGAAGATACGGCGGAAGCCGCGGCCGCGCACCAGGCAAACTCCACCTCCGGCCTCCACAGCCCAGATACGAAAGTAACATCATCAACTCCAAAAATATGGAAGAGCCAGACCAGCTTGCCTTACACCACTCTCTTAAGACACGTTCATGGAGTTCTGAATATTATAGGATGGGGAACTTTGATGCCCAGTGGGATTATGATCGCCAGATACTTGAGAGAATTCCCCATAAGCTGCGAGCGATGGTATTCGGCTCACGCTGTGTGCCAAACTGCTGGGTATATCATGGGCATCGTCGGATGGGGCTTCGGAATCTCCGCCGCTAACTCCTCCGAACGCAGCTCTCGCCTACCATTCTTAGCCCTCGGCATTACCATCATCGTCCTTGCAACCATTCAGCTGCTTGCTATATGTGTACAGCCGAAGAAGGAGAGCGGGTGGCGGCGGAGGTGGGAAATGTACCACCATGTTATGGGATACGTGATAATGGGACTGATAATAGGCGACATATTCGAAGGTATTAATACGCAGCGCCACCCAGAGAAATGGCGGTGGACTTACGTGGGAATTGTTTCTTTTCTTGCACTTGTGGCTGCGGCTTTGGAGATTCACAAACGAATCAAGCTTAAACTCTTCAAACAGGCTATGAAACTCAATGCCAATATGCATTCTCCCACTACTTGA

Protein sequence

MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT
Homology
BLAST of Moc01g30490 vs. NCBI nr
Match: XP_022132645.1 (cytochrome b561 and DOMON domain-containing protein At5g35735-like [Momordica charantia])

HSP 1 Score: 566.2 bits (1458), Expect = 1.5e-157
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA 60
           MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA
Sbjct: 1   MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA 60

Query: 61  HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR 120
           HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR
Sbjct: 61  HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR 120

Query: 121 YLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLA 180
           YLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLA
Sbjct: 121 YLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLA 180

Query: 181 TIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGI 240
           TIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGI
Sbjct: 181 TIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGI 240

Query: 241 VSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           VSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT
Sbjct: 241 VSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 277

BLAST of Moc01g30490 vs. NCBI nr
Match: XP_038883703.1 (cytochrome b561 and DOMON domain-containing protein At4g17280-like [Benincasa hispida])

HSP 1 Score: 329.3 bits (843), Expect = 3.1e-86
Identity = 178/278 (64.03%), Postives = 204/278 (73.38%), Query Frame = 0

Query: 1   MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA 60
           M S  H  L+LSL+   +CS   STP                             EA A 
Sbjct: 1   MGSRFHFFLILSLVVLAICSMGCSTP----------------------------TEARAE 60

Query: 61  HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR 120
            QANSTS +H+PD KVTS++ K+WKS   L YTTLLRHVHGVLN+IGWGTLMP GI++AR
Sbjct: 61  QQANSTS-VHNPDAKVTSAS-KVWKS-PGLGYTTLLRHVHGVLNMIGWGTLMPIGIIVAR 120

Query: 121 YLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVL 180
           Y R EFP++C++WYS HAVCQT GYIMG VGWGFG+S  NSS+R S   FL LGI +I+L
Sbjct: 121 YFRQEFPMNCDQWYSVHAVCQTCGYIMGTVGWGFGLSILNSSKR-SHPSFLILGIFVILL 180

Query: 181 ATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVG 240
           ATIQ+ AICVQPKKESG RR WE+YHHVMGYVIM LII DIFEGIN QRHPEKWRW YVG
Sbjct: 181 ATIQMFAICVQPKKESGRRRWWEIYHHVMGYVIMTLIIADIFEGINAQRHPEKWRWAYVG 240

Query: 241 IVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           I+S LALVAAALE+++ IKLKLFKQAMKLNANM+S TT
Sbjct: 241 ILSALALVAAALELNRCIKLKLFKQAMKLNANMYSHTT 246

BLAST of Moc01g30490 vs. NCBI nr
Match: XP_008440031.1 (PREDICTED: cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 [Cucumis melo])

HSP 1 Score: 327.0 bits (837), Expect = 1.6e-85
Identity = 163/225 (72.44%), Postives = 187/225 (83.11%), Query Frame = 0

Query: 56  EAAAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSG 115
           EA A  QANSTSGLH+PD KVTS + KIWK+   L YTTLLRHVHGVLNIIGWGTL+P G
Sbjct: 28  EATAEQQANSTSGLHNPDAKVTSPS-KIWKN-PGLGYTTLLRHVHGVLNIIGWGTLIPIG 87

Query: 116 IMIARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGI 175
           I++ RY R EFPI C++WYS HAVCQT GYIMG VGW FG+S  +SS + S LPFL LGI
Sbjct: 88  IIVGRYFRQEFPIRCDQWYSVHAVCQTCGYIMGTVGWAFGVSVLHSSSKRSHLPFLVLGI 147

Query: 176 TIIVLATIQLL-AICVQPKKESGWRRR-WEMYHHVMGYVIMGLIIGDIFEGINTQRHPEK 235
            II+L TIQ++ A+C+QPKKESG RRR WE+YHHVMGYVIM LIIG IFEGIN QRHP+K
Sbjct: 148 FIILLTTIQIMFAVCMQPKKESGARRRCWEIYHHVMGYVIMALIIGVIFEGINAQRHPKK 207

Query: 236 WRWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           WRW YVGI+S LA+V AALE+H+  +LKLFKQAMKLNANM+SPTT
Sbjct: 208 WRWGYVGILSGLAIVGAALEVHRCYRLKLFKQAMKLNANMYSPTT 250

BLAST of Moc01g30490 vs. NCBI nr
Match: XP_023544277.1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.8 bits (808), Expect = 3.6e-82
Identity = 157/224 (70.09%), Postives = 182/224 (81.25%), Query Frame = 0

Query: 55  AEAAAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPS 114
           +++ AA Q N+TSG H+PDTKVTS+ PKIWKS   L Y+TLLR VHGVLNIIGWGTL+P 
Sbjct: 18  SDSTAAQQENTTSGHHTPDTKVTSA-PKIWKS-PGLGYSTLLRQVHGVLNIIGWGTLIPI 77

Query: 115 GIMIARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALG 174
           GI+IARY R EFP+ C+RWYSAHAVCQT GYIMG VGWGFG+S  NSS R S +PFL LG
Sbjct: 78  GIVIARYFREEFPLKCDRWYSAHAVCQTCGYIMGTVGWGFGVSLINSSNR-SHVPFLVLG 137

Query: 175 ITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKW 234
           I +I+L  IQ+  ICVQ KKESG R RWEM+H  MGYVI+ LII DIFEGIN QRHP+KW
Sbjct: 138 ILVILLTAIQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIADIFEGINAQRHPKKW 197

Query: 235 RWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           RWTYVGI+  L +VAA LE+++ IKLK FKQAMKLNANM+S TT
Sbjct: 198 RWTYVGILLALGVVAAGLEVYRYIKLKRFKQAMKLNANMYSSTT 238

BLAST of Moc01g30490 vs. NCBI nr
Match: XP_023544278.1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.5 bits (807), Expect = 4.7e-82
Identity = 157/224 (70.09%), Postives = 182/224 (81.25%), Query Frame = 0

Query: 55  AEAAAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPS 114
           +++ AA Q N+TSG H+PDTKVTS+ PKIWKS   L Y+TLLR VHGVLNIIGWGTL+P 
Sbjct: 18  SDSTAAQQENTTSGHHTPDTKVTSA-PKIWKS-PGLGYSTLLRXVHGVLNIIGWGTLIPI 77

Query: 115 GIMIARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALG 174
           GI+IARY R EFP+ C+RWYSAHAVCQT GYIMG VGWGFG+S  NSS R S +PFL LG
Sbjct: 78  GIVIARYFREEFPLKCDRWYSAHAVCQTCGYIMGTVGWGFGVSLINSSNR-SHVPFLVLG 137

Query: 175 ITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKW 234
           I +I+L  IQ+  ICVQ KKESG R RWEM+H  MGYVI+ LII DIFEGIN QRHP+KW
Sbjct: 138 ILVILLTAIQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIADIFEGINAQRHPKKW 197

Query: 235 RWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           RWTYVGI+  L +VAA LE+++ IKLK FKQAMKLNANM+S TT
Sbjct: 198 RWTYVGILLALGVVAAGLEVYRYIKLKRFKQAMKLNANMYSSTT 238

BLAST of Moc01g30490 vs. ExPASy Swiss-Prot
Match: Q9FKH6 (Cytochrome b561 and DOMON domain-containing protein At5g35735 OS=Arabidopsis thaliana OX=3702 GN=At5g35735 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.2e-32
Identity = 63/156 (40.38%), Postives = 91/156 (58.33%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R+ HGVLN + WG LMP G M+ARY++ F  +   W+  H   Q +GY++G+ GW  GI 
Sbjct: 208 RNTHGVLNAVSWGVLMPMGAMMARYMKVF--ADPTWFYLHIAFQVSGYVIGVAGWATGIK 267

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N S  +S      LGI +   AT+Q+ A+ V+PK +  +R  W +YHH +GY  + L 
Sbjct: 268 LGNDSPGTSYSTHRNLGIALFTFATLQVFALLVRPKPDHKYRTYWNVYHHTVGYTTIILS 327

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           I +IF+G +     +KWRW Y+GI+ FL      LE
Sbjct: 328 IVNIFKGFDILDPEDKWRWAYIGILIFLGACVLILE 361

BLAST of Moc01g30490 vs. ExPASy Swiss-Prot
Match: Q9LSE7 (Cytochrome b561 and DOMON domain-containing protein At3g25290 OS=Arabidopsis thaliana OX=3702 GN=At3g25290 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.1e-31
Identity = 57/156 (36.54%), Postives = 94/156 (60.26%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG L P G +IARY+R F  +   W+  H  CQ + Y++G+ GW  G+ 
Sbjct: 217 RNIHGILNAVSWGILFPIGAIIARYMRVFDSADPAWFYLHVSCQFSAYVIGVAGWATGLK 276

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N SE         +GI +  LATIQ+ A+ ++PKK+  +R  W +YHH +GY I+ L 
Sbjct: 277 LGNESEGIRFSAHRNIGIALFTLATIQMFAMLLRPKKDHKYRFYWNIYHHGVGYAILTLG 336

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           I ++F+G+N  +  + ++  Y+ +++ L  +A  LE
Sbjct: 337 IINVFKGLNILKPQDTYKTAYIAVIAVLGGIALLLE 372

BLAST of Moc01g30490 vs. ExPASy Swiss-Prot
Match: Q8VYH6 (Cytochrome b561 and DOMON domain-containing protein At4g17280 OS=Arabidopsis thaliana OX=3702 GN=At4g17280 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.7e-31
Identity = 59/156 (37.82%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG +MP G +IARYLR    +   W+  H  CQ + YI+G+ GW  G+ 
Sbjct: 216 RNIHGILNGVSWGIMMPLGAIIARYLRVAKSADPAWFYIHVFCQASAYIIGVAGWATGLK 275

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
               S         A+GI +  LAT+Q+ A+ ++PK E   R  W +YHH +GY I+ L 
Sbjct: 276 LGGDSPGIQYSTHRAIGIALFSLATVQVFAMFLRPKPEHKHRLYWNIYHHTIGYTIIILG 335

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           + ++F+G+      ++W+  Y+GI+  LA+VA  LE
Sbjct: 336 VVNVFKGLGILSPKKQWKNAYIGIIVVLAIVATLLE 371

BLAST of Moc01g30490 vs. ExPASy Swiss-Prot
Match: Q9FGK4 (Cytochrome b561 and DOMON domain-containing protein At5g47530 OS=Arabidopsis thaliana OX=3702 GN=At5g47530 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 58/156 (37.18%), Postives = 93/156 (59.62%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG +MP G +IARYL+    +   W+  H  CQ++ YI+G+ GW  G+ 
Sbjct: 208 RNIHGILNGVSWGIMMPIGAIIARYLKVSKSADPAWFYLHVFCQSSAYIIGVAGWATGLK 267

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N S         A+GI +  LATIQ+ A+ ++PK E  +R  W +YHH +GY ++ L 
Sbjct: 268 LGNESAGIQFTFHRAVGIALFCLATIQVFAMFLRPKPEHKYRVYWNIYHHTVGYSVIILA 327

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           + ++F+G++     ++WR  Y  I+  L +VA  LE
Sbjct: 328 VVNVFKGLDILSPEKQWRNAYTAIIVVLGIVAVVLE 363

BLAST of Moc01g30490 vs. ExPASy Swiss-Prot
Match: Q9SV71 (Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis thaliana OX=3702 GN=At4g12980 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.4e-27
Identity = 51/157 (32.48%), Postives = 91/157 (57.96%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG L P G MIARY+R F  +   W+  H  CQ + Y++G+ GW  G+ 
Sbjct: 218 RNIHGILNAVSWGLLFPIGAMIARYMRIFESADPAWFYLHVSCQFSAYVIGVAGWATGLK 277

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             + S+         +GI +  +AT+Q+ A+ ++P+K+  +R  W +YHH +GY I+ L 
Sbjct: 278 LGSESKGIQYNTHRNIGICLFSIATLQMFAMLLRPRKDHKFRFVWNIYHHGVGYSILILG 337

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALEI 254
           I ++F+G++       ++  Y+ ++  L  +   LE+
Sbjct: 338 IINVFKGLSILNPKHTYKTAYIAVIGTLGGITLLLEV 374

BLAST of Moc01g30490 vs. ExPASy TrEMBL
Match: A0A6J1BT20 (cytochrome b561 and DOMON domain-containing protein At5g35735-like OS=Momordica charantia OX=3673 GN=LOC111005454 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 7.4e-158
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA 60
           MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA
Sbjct: 1   MASGSHCSLLLSLIFSVLCSTVYSTPCNSYGQDHEYCNNSLITKIGRDNKIEDTAEAAAA 60

Query: 61  HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR 120
           HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR
Sbjct: 61  HQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIMIAR 120

Query: 121 YLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLA 180
           YLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLA
Sbjct: 121 YLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITIIVLA 180

Query: 181 TIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGI 240
           TIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGI
Sbjct: 181 TIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWTYVGI 240

Query: 241 VSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           VSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT
Sbjct: 241 VSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 277

BLAST of Moc01g30490 vs. ExPASy TrEMBL
Match: A0A1S3B066 (cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484629 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 7.5e-86
Identity = 163/225 (72.44%), Postives = 187/225 (83.11%), Query Frame = 0

Query: 56  EAAAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSG 115
           EA A  QANSTSGLH+PD KVTS + KIWK+   L YTTLLRHVHGVLNIIGWGTL+P G
Sbjct: 28  EATAEQQANSTSGLHNPDAKVTSPS-KIWKN-PGLGYTTLLRHVHGVLNIIGWGTLIPIG 87

Query: 116 IMIARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGI 175
           I++ RY R EFPI C++WYS HAVCQT GYIMG VGW FG+S  +SS + S LPFL LGI
Sbjct: 88  IIVGRYFRQEFPIRCDQWYSVHAVCQTCGYIMGTVGWAFGVSVLHSSSKRSHLPFLVLGI 147

Query: 176 TIIVLATIQLL-AICVQPKKESGWRRR-WEMYHHVMGYVIMGLIIGDIFEGINTQRHPEK 235
            II+L TIQ++ A+C+QPKKESG RRR WE+YHHVMGYVIM LIIG IFEGIN QRHP+K
Sbjct: 148 FIILLTTIQIMFAVCMQPKKESGARRRCWEIYHHVMGYVIMALIIGVIFEGINAQRHPKK 207

Query: 236 WRWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           WRW YVGI+S LA+V AALE+H+  +LKLFKQAMKLNANM+SPTT
Sbjct: 208 WRWGYVGILSGLAIVGAALEVHRCYRLKLFKQAMKLNANMYSPTT 250

BLAST of Moc01g30490 vs. ExPASy TrEMBL
Match: A0A6J1INM1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478057 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 3.0e-82
Identity = 158/221 (71.49%), Postives = 179/221 (81.00%), Query Frame = 0

Query: 58  AAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIM 117
           + A Q  +T+G H+PDTKVTS+ PKIWKS   L Y+TLLRHVHGVLNIIGWGTL+P GI+
Sbjct: 20  STAVQQQNTTGHHTPDTKVTSA-PKIWKS-PGLGYSTLLRHVHGVLNIIGWGTLIPIGIV 79

Query: 118 IARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITI 177
           IARY R EFP+ C+RWYSAHAVCQT GYIMG VGWGFG+S  NSS R S +PFL LGI I
Sbjct: 80  IARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLINSSNR-SHIPFLVLGILI 139

Query: 178 IVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKWRWT 237
           IVL  IQ+  ICVQ KKESG R RWEM+H  MGYVI+ LII DIFEGIN QRHP+KWRW 
Sbjct: 140 IVLTAIQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIADIFEGINAQRHPKKWRWA 199

Query: 238 YVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           YVGI+  L +VAAALE++  IKLK FKQAMKLNANM+SPTT
Sbjct: 200 YVGILLALVVVAAALEVYTYIKLKRFKQAMKLNANMYSPTT 237

BLAST of Moc01g30490 vs. ExPASy TrEMBL
Match: A0A0A0KHA4 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G513490 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 8.6e-82
Identity = 159/225 (70.67%), Postives = 178/225 (79.11%), Query Frame = 0

Query: 56  EAAAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSG 115
           EA +  QANSTSGLH PD KVTS  P        L Y TLLRHVHG+LNIIGWGTL+P G
Sbjct: 28  EATSEQQANSTSGLHDPDAKVTS--PSKTLKNPGLGYVTLLRHVHGILNIIGWGTLIPIG 87

Query: 116 IMIARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGI 175
           I+I RY R EFPI C++WYS HAVCQT GYIMG VGW FG+S  +SS + S LPFL LGI
Sbjct: 88  IIIGRYFRHEFPIRCDQWYSIHAVCQTCGYIMGTVGWAFGVSVLHSSSKRSYLPFLVLGI 147

Query: 176 TIIVLATIQ-LLAICVQPKKESGWRRR-WEMYHHVMGYVIMGLIIGDIFEGINTQRHPEK 235
            II+L TIQ +LAICVQ KKESG RRR WE +HHVMGYVIM LIIG IFEGIN QRHP+K
Sbjct: 148 FIILLTTIQIMLAICVQSKKESGERRRCWEKHHHVMGYVIMALIIGVIFEGINAQRHPKK 207

Query: 236 WRWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           WRW YVGI+S LA+V AALE+H+  KLKLFKQAMKLNANM+SP+T
Sbjct: 208 WRWCYVGILSGLAIVGAALEVHRCYKLKLFKQAMKLNANMYSPST 250

BLAST of Moc01g30490 vs. ExPASy TrEMBL
Match: A0A6J1ISN4 (cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478057 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.2e-80
Identity = 158/224 (70.54%), Postives = 179/224 (79.91%), Query Frame = 0

Query: 58  AAAHQANSTSGLHSPDTKVTSSTPKIWKSQTSLPYTTLLRHVHGVLNIIGWGTLMPSGIM 117
           + A Q  +T+G H+PDTKVTS+ PKIWKS   L Y+TLLRHVHGVLNIIGWGTL+P GI+
Sbjct: 20  STAVQQQNTTGHHTPDTKVTSA-PKIWKS-PGLGYSTLLRHVHGVLNIIGWGTLIPIGIV 79

Query: 118 IARYLR-EFPISCERWYSAHAVCQTAGYIMGIVGWGFGISAANSSERSSRLPFLALGITI 177
           IARY R EFP+ C+RWYSAHAVCQT GYIMG VGWGFG+S  NSS R S +PFL LGI I
Sbjct: 80  IARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLINSSNR-SHIPFLVLGILI 139

Query: 178 IVLATI---QLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLIIGDIFEGINTQRHPEKW 237
           IVL  I   Q+  ICVQ KKESG R RWEM+H  MGYVI+ LII DIFEGIN QRHP+KW
Sbjct: 140 IVLTAIQSWQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIADIFEGINAQRHPKKW 199

Query: 238 RWTYVGIVSFLALVAAALEIHKRIKLKLFKQAMKLNANMHSPTT 278
           RW YVGI+  L +VAAALE++  IKLK FKQAMKLNANM+SPTT
Sbjct: 200 RWAYVGILLALVVVAAALEVYTYIKLKRFKQAMKLNANMYSPTT 240

BLAST of Moc01g30490 vs. TAIR 10
Match: AT5G35735.1 (Auxin-responsive family protein )

HSP 1 Score: 141.7 bits (356), Expect = 8.6e-34
Identity = 63/156 (40.38%), Postives = 91/156 (58.33%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R+ HGVLN + WG LMP G M+ARY++ F  +   W+  H   Q +GY++G+ GW  GI 
Sbjct: 208 RNTHGVLNAVSWGVLMPMGAMMARYMKVF--ADPTWFYLHIAFQVSGYVIGVAGWATGIK 267

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N S  +S      LGI +   AT+Q+ A+ V+PK +  +R  W +YHH +GY  + L 
Sbjct: 268 LGNDSPGTSYSTHRNLGIALFTFATLQVFALLVRPKPDHKYRTYWNVYHHTVGYTTIILS 327

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           I +IF+G +     +KWRW Y+GI+ FL      LE
Sbjct: 328 IVNIFKGFDILDPEDKWRWAYIGILIFLGACVLILE 361

BLAST of Moc01g30490 vs. TAIR 10
Match: AT3G25290.1 (Auxin-responsive family protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.6e-32
Identity = 57/156 (36.54%), Postives = 94/156 (60.26%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG L P G +IARY+R F  +   W+  H  CQ + Y++G+ GW  G+ 
Sbjct: 217 RNIHGILNAVSWGILFPIGAIIARYMRVFDSADPAWFYLHVSCQFSAYVIGVAGWATGLK 276

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N SE         +GI +  LATIQ+ A+ ++PKK+  +R  W +YHH +GY I+ L 
Sbjct: 277 LGNESEGIRFSAHRNIGIALFTLATIQMFAMLLRPKKDHKYRFYWNIYHHGVGYAILTLG 336

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           I ++F+G+N  +  + ++  Y+ +++ L  +A  LE
Sbjct: 337 IINVFKGLNILKPQDTYKTAYIAVIAVLGGIALLLE 372

BLAST of Moc01g30490 vs. TAIR 10
Match: AT3G25290.2 (Auxin-responsive family protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.6e-32
Identity = 57/156 (36.54%), Postives = 94/156 (60.26%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG L P G +IARY+R F  +   W+  H  CQ + Y++G+ GW  G+ 
Sbjct: 217 RNIHGILNAVSWGILFPIGAIIARYMRVFDSADPAWFYLHVSCQFSAYVIGVAGWATGLK 276

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N SE         +GI +  LATIQ+ A+ ++PKK+  +R  W +YHH +GY I+ L 
Sbjct: 277 LGNESEGIRFSAHRNIGIALFTLATIQMFAMLLRPKKDHKYRFYWNIYHHGVGYAILTLG 336

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           I ++F+G+N  +  + ++  Y+ +++ L  +A  LE
Sbjct: 337 IINVFKGLNILKPQDTYKTAYIAVIAVLGGIALLLE 372

BLAST of Moc01g30490 vs. TAIR 10
Match: AT4G17280.1 (Auxin-responsive family protein )

HSP 1 Score: 135.6 bits (340), Expect = 6.2e-32
Identity = 59/156 (37.82%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG +MP G +IARYLR    +   W+  H  CQ + YI+G+ GW  G+ 
Sbjct: 216 RNIHGILNGVSWGIMMPLGAIIARYLRVAKSADPAWFYIHVFCQASAYIIGVAGWATGLK 275

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
               S         A+GI +  LAT+Q+ A+ ++PK E   R  W +YHH +GY I+ L 
Sbjct: 276 LGGDSPGIQYSTHRAIGIALFSLATVQVFAMFLRPKPEHKHRLYWNIYHHTIGYTIIILG 335

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           + ++F+G+      ++W+  Y+GI+  LA+VA  LE
Sbjct: 336 VVNVFKGLGILSPKKQWKNAYIGIIVVLAIVATLLE 371

BLAST of Moc01g30490 vs. TAIR 10
Match: AT5G47530.1 (Auxin-responsive family protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 58/156 (37.18%), Postives = 93/156 (59.62%), Query Frame = 0

Query: 97  RHVHGVLNIIGWGTLMPSGIMIARYLREFPISCERWYSAHAVCQTAGYIMGIVGWGFGIS 156
           R++HG+LN + WG +MP G +IARYL+    +   W+  H  CQ++ YI+G+ GW  G+ 
Sbjct: 208 RNIHGILNGVSWGIMMPIGAIIARYLKVSKSADPAWFYLHVFCQSSAYIIGVAGWATGLK 267

Query: 157 AANSSERSSRLPFLALGITIIVLATIQLLAICVQPKKESGWRRRWEMYHHVMGYVIMGLI 216
             N S         A+GI +  LATIQ+ A+ ++PK E  +R  W +YHH +GY ++ L 
Sbjct: 268 LGNESAGIQFTFHRAVGIALFCLATIQVFAMFLRPKPEHKYRVYWNIYHHTVGYSVIILA 327

Query: 217 IGDIFEGINTQRHPEKWRWTYVGIVSFLALVAAALE 253
           + ++F+G++     ++WR  Y  I+  L +VA  LE
Sbjct: 328 VVNVFKGLDILSPEKQWRNAYTAIIVVLGIVAVVLE 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132645.11.5e-157100.00cytochrome b561 and DOMON domain-containing protein At5g35735-like [Momordica ch... [more]
XP_038883703.13.1e-8664.03cytochrome b561 and DOMON domain-containing protein At4g17280-like [Benincasa hi... [more]
XP_008440031.11.6e-8572.44PREDICTED: cytochrome b561 and DOMON domain-containing protein At4g17280-like is... [more]
XP_023544277.13.6e-8270.09cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X1 [C... [more]
XP_023544278.14.7e-8270.09cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 [C... [more]
Match NameE-valueIdentityDescription
Q9FKH61.2e-3240.38Cytochrome b561 and DOMON domain-containing protein At5g35735 OS=Arabidopsis tha... [more]
Q9LSE75.1e-3136.54Cytochrome b561 and DOMON domain-containing protein At3g25290 OS=Arabidopsis tha... [more]
Q8VYH68.7e-3137.82Cytochrome b561 and DOMON domain-containing protein At4g17280 OS=Arabidopsis tha... [more]
Q9FGK41.9e-3037.18Cytochrome b561 and DOMON domain-containing protein At5g47530 OS=Arabidopsis tha... [more]
Q9SV713.4e-2732.48Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1BT207.4e-158100.00cytochrome b561 and DOMON domain-containing protein At5g35735-like OS=Momordica ... [more]
A0A1S3B0667.5e-8672.44cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS... [more]
A0A6J1INM13.0e-8271.49cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 OS... [more]
A0A0A0KHA48.6e-8270.67Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G51... [more]
A0A6J1ISN41.2e-8070.54cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS... [more]
Match NameE-valueIdentityDescription
AT5G35735.18.6e-3440.38Auxin-responsive family protein [more]
AT3G25290.13.6e-3236.54Auxin-responsive family protein [more]
AT3G25290.23.6e-3236.54Auxin-responsive family protein [more]
AT4G17280.16.2e-3237.82Auxin-responsive family protein [more]
AT5G47530.11.4e-3137.18Auxin-responsive family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006593Cytochrome b561/ferric reductase transmembraneSMARTSM00665561_7coord: 99..224
e-value: 6.2E-23
score: 92.2
IPR006593Cytochrome b561/ferric reductase transmembranePFAMPF03188Cytochrom_B561coord: 99..227
e-value: 6.4E-8
score: 32.8
IPR006593Cytochrome b561/ferric reductase transmembranePROSITEPS50939CYTOCHROME_B561coord: 58..260
score: 22.544941
NoneNo IPR availableGENE3D1.20.120.1770coord: 77..259
e-value: 5.9E-22
score: 79.9
NoneNo IPR availablePANTHERPTHR23130CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEINcoord: 5..275
NoneNo IPR availablePANTHERPTHR23130:SF153FAMILY PROTEIN, PUTATIVE-RELATEDcoord: 5..275
NoneNo IPR availableCDDcd08760Cyt_b561_FRRS1_likecoord: 74..253
e-value: 1.06452E-35
score: 124.765

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g30490.1Moc01g30490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016829 lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding