Moc01g30290 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g30290
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	alkaline ceramidase
Location	chr1: 21361356 .. 21362363 (-)
RNA-Seq Expression	Moc01g30290
Synteny	Moc01g30290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGATGGAATATCGAGTTTCTGGGGTCCTGTCACATCTACAATGGAGTGTTGCGAGAAAAATTATGCTTATTCTACTTATGTTGCAGAGTTTTTTAACACAATATCAAATATACCAACCATTCTATTGGCTCTGATCGGTCTTTCAAATGCGCTGAAACAACGGTTCGAGAAGAGATTTAGTGTACTTCATATATCGAATATGATATTGGCCATCGGTAGTATGTTCTACCATGCCACATTGCAAAAACTGTAAGTCCATATTGGCCATATATCGAATATGATATTGAATATGATATTGGCCATTGGTAGAAATGATGCATAATGTGATTTGATCAGCTACTTACATAATTTAGGTGTTCTGATTGCAGTTTTATCCATTTTCTGTGGCTGGTGAAATTATAGATATGTTTATGAAATCTGTGGTCTTGAATGTTCTTTTGCAACTGAATTTTGGCAATCAATGATCAAACGCTTTTGTTTTCTAACCAGGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAGATGCTTCTTTACATGTACATTCTCTATTCACCCGATTGGCATTACCGAAGTACAATGCCAACATTCCTCTTCCTCTATGGAGCCATTTTTGCCGTGGCTCACTCGATTCTTCGTTATGGAATTGGTTTCAAGGTGCATTATGTGATTCTGTGTCTCCTCTGCATACCTCGGATGTACAAGTACTATATATACACAGAAGACGCCTCTGCTAAGCGGCTCGCGAGGCTGTACTTGTTGACTCTTTTCTTAGGCATTCTCTGTTGGGTTATCGATCGGGGTTTCTGCGAGAAGATATCGCATTGGATGATCAACCCTCAGGGCCATGCCTTGTGGCATGTTTTCATGGGGTTGGGTTCATACTTTGCAAACACATTCCTAATGTTTTGCCGAGCGCAGCAAAGAGGATGGGCCCCTAAAGTTTTACATTTGATGGGGATTCTCCCTTATGTGAAGATTGAGAAACCGAAAACCCAGTAA

mRNA sequence

ATGGCTGATGGAATATCGAGTTTCTGGGGTCCTGTCACATCTACAATGGAGTGTTGCGAGAAAAATTATGCTTATTCTACTTATGTTGCAGAGTTTTTTAACACAATATCAAATATACCAACCATTCTATTGGCTCTGATCGGTCTTTCAAATGCGCTGAAACAACGGTTCGAGAAGAGATTTAGTGTACTTCATATATCGAATATGATATTGGCCATCGGTAGTATGTTCTACCATGCCACATTGCAAAAACTGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAGATGCTTCTTTACATGTACATTCTCTATTCACCCGATTGGCATTACCGAAGTACAATGCCAACATTCCTCTTCCTCTATGGAGCCATTTTTGCCGTGGCTCACTCGATTCTTCGTTATGGAATTGGTTTCAAGGTGCATTATGTGATTCTGTGTCTCCTCTGCATACCTCGGATGTACAAGTACTATATATACACAGAAGACGCCTCTGCTAAGCGGCTCGCGAGGCTGTACTTGTTGACTCTTTTCTTAGGCATTCTCTGTTGGGTTATCGATCGGGGTTTCTGCGAGAAGATATCGCATTGGATGATCAACCCTCAGGGCCATGCCTTGTGGCATGTTTTCATGGGGTTGGGTTCATACTTTGCAAACACATTCCTAATGTTTTGCCGAGCGCAGCAAAGAGGATGGGCCCCTAAAGTTTTACATTTGATGGGGATTCTCCCTTATGTGAAGATTGAGAAACCGAAAACCCAGTAA

Coding sequence (CDS)

ATGGCTGATGGAATATCGAGTTTCTGGGGTCCTGTCACATCTACAATGGAGTGTTGCGAGAAAAATTATGCTTATTCTACTTATGTTGCAGAGTTTTTTAACACAATATCAAATATACCAACCATTCTATTGGCTCTGATCGGTCTTTCAAATGCGCTGAAACAACGGTTCGAGAAGAGATTTAGTGTACTTCATATATCGAATATGATATTGGCCATCGGTAGTATGTTCTACCATGCCACATTGCAAAAACTGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAGATGCTTCTTTACATGTACATTCTCTATTCACCCGATTGGCATTACCGAAGTACAATGCCAACATTCCTCTTCCTCTATGGAGCCATTTTTGCCGTGGCTCACTCGATTCTTCGTTATGGAATTGGTTTCAAGGTGCATTATGTGATTCTGTGTCTCCTCTGCATACCTCGGATGTACAAGTACTATATATACACAGAAGACGCCTCTGCTAAGCGGCTCGCGAGGCTGTACTTGTTGACTCTTTTCTTAGGCATTCTCTGTTGGGTTATCGATCGGGGTTTCTGCGAGAAGATATCGCATTGGATGATCAACCCTCAGGGCCATGCCTTGTGGCATGTTTTCATGGGGTTGGGTTCATACTTTGCAAACACATTCCTAATGTTTTGCCGAGCGCAGCAAAGAGGATGGGCCCCTAAAGTTTTACATTTGATGGGGATTCTCCCTTATGTGAAGATTGAGAAACCGAAAACCCAGTAA

Protein sequence

MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKRFSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFLGILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHLMGILPYVKIEKPKTQ

Homology

[Close]

BLAST of Moc01g30290 vs. NCBI nr
Match: XP_022132606.1 (alkaline ceramidase [Momordica charantia] >XP_022132607.1 alkaline ceramidase [Momordica charantia])

HSP 1 Score: 530.8 bits (1366), Expect = 6.5e-147
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR
Sbjct: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL
Sbjct: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. NCBI nr
Match: XP_023003815.1 (alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucurbita maxima] >XP_023003817.1 alkaline ceramidase [Cucurbita maxima] >XP_023003819.1 alkaline ceramidase [Cucurbita maxima] >XP_023003820.1 alkaline ceramidase [Cucurbita maxima])

HSP 1 Score: 503.1 bits (1294), Expect = 1.5e-138
Identity = 238/255 (93.33%), Postives = 248/255 (97.25%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCEKNYA+S+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDRGFCEKISHW INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. NCBI nr
Match: KAG7026660.1 (Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.1 bits (1289), Expect = 5.5e-138
Identity = 238/255 (93.33%), Postives = 247/255 (96.86%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCEKNYAYS+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDR FCEKISHW INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCFCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. NCBI nr
Match: XP_022926905.1 (alkaline ceramidase [Cucurbita moschata] >XP_022926906.1 alkaline ceramidase [Cucurbita moschata] >XP_022926907.1 alkaline ceramidase [Cucurbita moschata] >XP_022926908.1 alkaline ceramidase [Cucurbita moschata])

HSP 1 Score: 498.8 bits (1283), Expect = 2.7e-137
Identity = 237/255 (92.94%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCEKNYAYS+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDR  CEKISHW INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCLCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. NCBI nr
Match: KAG6594691.1 (Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 498.8 bits (1283), Expect = 2.7e-137
Identity = 237/255 (92.94%), Postives = 247/255 (96.86%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCEKNYAYS+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDR FCEKIS+W INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCFCEKISYWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. ExPASy Swiss-Prot
Match: Q94IB9 (Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 3.1e-123
Identity = 202/255 (79.22%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCE NYAYS+Y+AEF+NTISN+P ILLALIGL NAL+QRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FS+LHISNMILAIGSM YHATLQ +QQQ DETPM+WE+LLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGA FA+ H+ LR+GIGFKVHYVILCLLCIPRMYKYYI+TED +AKR+A+ Y+ T+ +
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           G +CW  DR FC+ IS W +NPQGHALWHVFM   SY ANTFLMFCRAQQRGW PKV + 
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 MGILPYVKIEKPKTQ 256
           +G+LPYVKIEKPKTQ
Sbjct: 241 LGVLPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. ExPASy Swiss-Prot
Match: Q9D099 (Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.4e-29
Identity = 84/259 (32.43%), Postives = 130/259 (50.19%), Query Frame = 0


Query: 8   FWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKRFSVLHIS 67
           +WGP TST++ CE+NY  + +VAEF+NT+SN+  I+  + G    ++ R EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLA 69

Query: 68  NMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PMI+   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKTKSSINYHLLFT----L 129

Query: 128 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLF- 187
           FLY       +  ++  I  +V Y +L    + R    YI T      R      LT+F 
Sbjct: 130 FLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLR--SIYIVTWVYPWLRGLGYTSLTVFL 189

Query: 188 LGILCWVIDRGFCEKISHWM--------INPQGHALWHVFMGLGSYFANTFLMFCRAQQR 247
           LG L W ID  FC+ + ++         +  Q HA WH+  GLGSY    F ++ R    
Sbjct: 190 LGFLLWNIDNIFCDSLRNFRKRVPPVLGVTTQFHAWWHILTGLGSYLHILFSLYTRTLYL 249

Query: 248 GWAPKVLHLMGILPYVKIE 251
            + PKV  L GI P V  E
Sbjct: 250 RYRPKVKFLFGIWPAVMFE 262

[Close]

BLAST of Moc01g30290 vs. ExPASy Swiss-Prot
Match: Q9NUN7 (Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3)

HSP 1 Score: 124.4 bits (311), Expect = 1.8e-27
Identity = 79/258 (30.62%), Postives = 128/258 (49.61%), Query Frame = 0


Query: 8   FWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKRFSVLHIS 67
           +WGP TST++ CE+NY+ + Y+AEF+NT+SN+  I+  + G   +++   EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLA 69

Query: 68  NMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PMI+   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHLLFT----L 129

Query: 128 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 187
            L+  I    +  ++  I  +V Y +L    + R   Y +       + L    L    L
Sbjct: 130 VLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSI-YIVTWVYPWLRGLGYTSLGIFLL 189

Query: 188 GILCWVIDRGFCEKISHWM--------INPQGHALWHVFMGLGSYFANTFLMFCRAQQRG 247
           G L W ID  FCE + ++         I  Q HA WH+  GLGSY    F ++ R     
Sbjct: 190 GFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTLYLR 249

Query: 248 WAPKVLHLMGILPYVKIE 251
           + PKV  L GI P +  E
Sbjct: 250 YRPKVKFLFGIWPVILFE 262

[Close]

BLAST of Moc01g30290 vs. ExPASy Swiss-Prot
Match: Q6TMJ1 (Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.7e-18
Identity = 72/273 (26.37%), Postives = 121/273 (44.32%), Query Frame = 0


Query: 9   WGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGL---------------SNAL 68
           +G  T++++ CE NY YS Y+AEF+NT S++   L  + G+                  L
Sbjct: 10  YGTPTASIDWCELNYTYSPYIAEFYNTFSSLIISLFGIYGIWIMMPNFGTGVEKEHIKIL 69

Query: 69  KQRFEKRFSVL-HISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWH- 128
           KQ   +   +L +IS +++ +GS FYHATL    Q  DE PMI+  L+ +YI+ +     
Sbjct: 70  KQLDVRNKVILSYISLIVVGVGSAFYHATLLYQNQLFDELPMIYTALIMLYIMVTVGEEK 129

Query: 129 --------------YRSTMPTFLFLYGAIFAVA-------HSILRYGIGFKVHYVILCLL 188
                          R  +P  L  YG    +          IL+   G  V YV+   +
Sbjct: 130 TKKGFKGGVLGNSLLRHLLPYLLIAYGLFVTITILVIQDQPKILQVSFGALVFYVVFHSI 189

Query: 189 CIPRMYKYYIYTEDASAKRLARLYLLTLFLGILCWVIDRGFCEKISHWMINPQGHALWHV 244
            +    K      +  +  L +   +++ +G  CWV++R FC+    +    Q HA WH 
Sbjct: 190 YLINKKKPDGMPSNPDS-YLYKYAFVSMLVGFTCWVVERYFCKNGKTFGF--QLHAFWHF 249

[Close]

BLAST of Moc01g30290 vs. ExPASy Swiss-Prot
Match: Q02896 (Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDC1 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.3e-16
Identity = 67/260 (25.77%), Postives = 117/260 (45.00%), Query Frame = 0


Query: 5   ISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKRFSVL 64
           I  +WG  TS ++ CE+NY  S Y+AE+ NTI+N   ++ A     +A + + E R+ ++
Sbjct: 12  IEGYWGKPTSLIDWCEENYVVSPYIAEWSNTITNSIFLMTAFYSTYSAWRNKLETRYILI 71

Query: 65  HISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYS-------------PDWH 124
            +   ++ IGS  +H TLQ   Q  DE PM++  ++  + +++              +  
Sbjct: 72  GMGFSLVGIGSWLFHMTLQYRYQLLDELPMLYATIIPSWSIFAETQEILIKDEKKRKESS 131

Query: 125 YRSTMPTFLFLYG--AIFAVAHSILRYGIGFKVHYVILCLLCIPRM-YKYYIYTEDASAK 184
           +R  M     + G   I    + +++    F+V Y IL LL +    +  Y +  D+ AK
Sbjct: 132 FRIQMVISFIMCGIVTILTWIYVVVQKPAIFQVLYGILTLLVVVLSGWLTYYHVHDSFAK 191

Query: 185 R----LARLYLLTLFLGILCWVIDRGFCEKISHW-------MINPQG-----HALWHVFM 233
           +       + ++   +G +CW +D   C   S W       +  P G     HA WH+  
Sbjct: 192 KNLFITMVMGMIPFVIGFICWQLDIHLC---SFWIYIRRTYLALPLGVLLELHAWWHLLT 251

[Close]

BLAST of Moc01g30290 vs. ExPASy TrEMBL
Match: A0A6J1BUA9 (alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.2e-147
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR
Sbjct: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL
Sbjct: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. ExPASy TrEMBL
Match: A0A6J1KSV2 (alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 7.0e-139
Identity = 238/255 (93.33%), Postives = 248/255 (97.25%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCEKNYA+S+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDRGFCEKISHW INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. ExPASy TrEMBL
Match: A0A6J1EME1 (alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.3e-137
Identity = 237/255 (92.94%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCEKNYAYS+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDR  CEKISHW INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCLCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. ExPASy TrEMBL
Match: A0A1S3B0Z4 (alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 3.9e-137
Identity = 233/255 (91.37%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST ECCE+NYAYS+Y+AEF+NT+SNIPTILLALIGL+NAL+QRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGA+FAVAHSILRY IGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLY LTL L
Sbjct: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           G  CWV DRG CEKISHW+INPQGHALWHVFMGL SY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGVLPYVKIEKPKTQ 255

[Close]

BLAST of Moc01g30290 vs. ExPASy TrEMBL
Match: A0A6P3ZI05 (alkaline ceramidase OS=Ziziphus jujuba OX=326968 GN=LOC107414703 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.5e-128
Identity = 213/255 (83.53%), Postives = 236/255 (92.55%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST ECCEKNYAYS+Y+AEF+NTISNIPTILLALIGL NAL+QRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCEKNYAYSSYIAEFYNTISNIPTILLALIGLINALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSM YHATLQ +QQQ DETPM+WEMLLYMYILYSPDWHYR TMPTFL
Sbjct: 61  FSVLHISNMILAIGSMLYHATLQHVQQQSDETPMVWEMLLYMYILYSPDWHYRGTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGA FA+ HS++R+GIGFKVHYV+LCLLCIPRMYKYYIYT+DA AKRLA+ Y+ T+FL
Sbjct: 121 FLYGAAFAIVHSVIRFGIGFKVHYVLLCLLCIPRMYKYYIYTQDACAKRLAKFYVATIFL 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           G LCW+ DR FC+++S W+INPQGHALWHVFMG  SYFANTFLMFCRAQQRGWAPK++H 
Sbjct: 181 GSLCWLFDRVFCKEVSQWLINPQGHALWHVFMGFNSYFANTFLMFCRAQQRGWAPKIVHF 240

Query: 241 MGILPYVKIEKPKTQ 256
           MG LPYVKIEKPK+Q
Sbjct: 241 MGFLPYVKIEKPKSQ 255

[Close]

BLAST of Moc01g30290 vs. TAIR 10
Match: AT4G22330.1 (Alkaline phytoceramidase (aPHC) )

HSP 1 Score: 442.6 bits (1137), Expect = 2.2e-124
Identity = 202/255 (79.22%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60
           MADGISSFWGPVTST+ECCE NYAYS+Y+AEF+NTISN+P ILLALIGL NAL+QRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FS+LHISNMILAIGSM YHATLQ +QQQ DETPM+WE+LLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180
           FLYGA FA+ H+ LR+GIGFKVHYVILCLLCIPRMYKYYI+TED +AKR+A+ Y+ T+ +
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240
           G +CW  DR FC+ IS W +NPQGHALWHVFM   SY ANTFLMFCRAQQRGW PKV + 
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 MGILPYVKIEKPKTQ 256
           +G+LPYVKIEKPKTQ
Sbjct: 241 LGVLPYVKIEKPKTQ 255

The following BLAST results are available for this feature:

BLAST of Moc01g30290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132606.1	6.5e-147	100.00	alkaline ceramidase [Momordica charantia] >XP_022132607.1 alkaline ceramidase [M...	[more]
XP_023003815.1	1.5e-138	93.33	alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucu...	[more]
KAG7026660.1	5.5e-138	93.33	Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022926905.1	2.7e-137	92.94	alkaline ceramidase [Cucurbita moschata] >XP_022926906.1 alkaline ceramidase [Cu...	[more]
KAG6594691.1	2.7e-137	92.94	Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Moc01g30290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IB9	3.1e-123	79.22	Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1	[more]
Q9D099	4.4e-29	32.43	Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1	[more]
Q9NUN7	1.8e-27	30.62	Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3	[more]
Q6TMJ1	2.7e-18	26.37	Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A...	[more]
Q02896	3.3e-16	25.77	Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]

BLAST of Moc01g30290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUA9	3.2e-147	100.00	alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1	[more]
A0A6J1KSV2	7.0e-139	93.33	alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1	[more]
A0A6J1EME1	1.3e-137	92.94	alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1	[more]
A0A1S3B0Z4	3.9e-137	91.37	alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1	[more]
A0A6P3ZI05	1.5e-128	83.53	alkaline ceramidase OS=Ziziphus jujuba OX=326968 GN=LOC107414703 PE=3 SV=1	[more]

BLAST of Moc01g30290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22330.1	2.2e-124	79.22	Alkaline phytoceramidase (aPHC)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008901	Alkaline ceramidase	PFAM	PF05875	Ceramidase	coord: 8..238 e-value: 4.1E-58 score: 196.9
IPR044219	Alkaline ceramidase, plant	PANTHER	PTHR46852	ALKALINE CERAMIDASE	coord: 1..255
None	No IPR available	PANTHER	PTHR46852:SF5	ALKALINE CERAMIDASE	coord: 1..255

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g30290.1	Moc01g30290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006672	ceramide metabolic process
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006914	autophagy
biological_process	GO:0010025	wax biosynthetic process
biological_process	GO:0030104	water homeostasis
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0030148	sphingolipid biosynthetic process
biological_process	GO:0031667	response to nutrient levels
biological_process	GO:0002238	response to molecule of fungal origin
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0090333	regulation of stomatal closure
biological_process	GO:0010508	positive regulation of autophagy
biological_process	GO:0010150	leaf senescence
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides