Moc01g30220 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g30220
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionU-box domain-containing protein 4
Locationchr1: 21312819 .. 21314282 (-)
RNA-Seq ExpressionMoc01g30220
SyntenyMoc01g30220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGTCGCTTGAGGATTCTCACTCCAGTTCCAATCGGTTTCCTTTGGGGAGAAGTTGCTACAGCCCATCTTCTACCACTTCCAGTAAAATTAGCAGGAACATTGGGCGTTCTATGCGCACTATTCGCTCCAATTTCTTTCAGGATGATAGTAGCTGCACTTTTAACGGTTCGGTAGCCGGAAAATCGGGCTGCGTCTCTGAGAATCTCACCGATTCTGTCATTGACCTTAGGCTTGGCGAGTTGGCATCGCGTAGCCCTACATGGGCCAAGCAATCGTCTGAACAGGAAGAGGATTTTCTTGAACTTTCTCATGCTTTCAGTGATTTCTCTGCTTGTAGCAGTGATATATCAGGCGAGTTGCAGAGGCTTGCAAGTTTGCCGTCCATGGCAGTTGTCCCTAGAAGAGAAGGGGAAGATGCCGAGCCCGAGCCCTGCTTAGGGTTTCTGCAGAGGGAGAATTTCTCCACCGAGATTATTGAGAGCATTTCGCCGGAAGATCTGCAGCCAACTGTTAAGATCTGCATCGACGGCCTCCAGTCGTCGTCAATTGCAGTCAAGCGATCTGCCGCGGCCAAGCTTAGGCTTCTGGCGAAGAACCGGTCCGACAACCGGGTACTCATTGGGGAGTCCGGCGCCGTCCCTGCTCTAATTCCTCTGCTTCGATCCAGCGATCCATGGACGCAAGAGCATGCTGTAACCGCACTGCTAAATCTCTCACTCCATGAGTCCAATAAGGTTATCATCACAAATGCTGGAGCCATAAAATTGCTGGTTTATGCACTCAAAACTGGTACGGAAACTTCGAAGCAGAATGCGGCGTGTGCTCTAATGAGTCTGGCTTTATTGGAGGAAAACAAGACTTCGATTGGGGTTTGCGGGGCCATCCCACCTCTGGTTTCTTTACTATTGAACGGATCGAATAGGGGAAAAAAGGACGCGCTCACAACGCTCTACAAGCTTTGCTCGATCAAGCCTAACAAGGAGCGGGCTGTCTCTGCCGGAGCTGTGAAGCCATTGGTGGCGCTGGTGGTGGAGCAAGGAACGGGTCTGGCAGAGAAGGCCATGGTGGTTCTGAGTAGCCTGGCTGGGATTCAGGAGGGCAAGGATTCGATTGTGGAAGAGGGGGGAATTGCTGCGCTCGTGGAAGCAATCGAAGATGGGTCGGTGAAAGGGAAAGAGTTTGCGGTATTGACACTCTTGCAATTGTGTGTTGAGAGTGTGAGAAATAGAGGGTTGCTCGTAAGGGAAGGCGGAATTCCACCTCTGGTTGCACTTTCGCAGACTGGAAGCGTTCGAGCAAAGCATAAGGTGATAACTAGAAACTGATTACTCGGCAAATTATTTACTTGATTCTATGAAATGAACACCTTGTTGATATCAATTCGCTAAATTTTGCAGGCGGAAACACTTCTGGGATATTTAAGAGAACCAAGACAAGAAGCATCCTCATCAAGTCCTTAA

mRNA sequence

ATGGTGTCGCTTGAGGATTCTCACTCCAGTTCCAATCGGTTTCCTTTGGGGAGAAGTTGCTACAGCCCATCTTCTACCACTTCCAGTAAAATTAGCAGGAACATTGGGCGTTCTATGCGCACTATTCGCTCCAATTTCTTTCAGGATGATAGTAGCTGCACTTTTAACGGTTCGGTAGCCGGAAAATCGGGCTGCGTCTCTGAGAATCTCACCGATTCTGTCATTGACCTTAGGCTTGGCGAGTTGGCATCGCGTAGCCCTACATGGGCCAAGCAATCGTCTGAACAGGAAGAGGATTTTCTTGAACTTTCTCATGCTTTCAGTGATTTCTCTGCTTGTAGCAGTGATATATCAGGCGAGTTGCAGAGGCTTGCAAGTTTGCCGTCCATGGCAGTTGTCCCTAGAAGAGAAGGGGAAGATGCCGAGCCCGAGCCCTGCTTAGGGTTTCTGCAGAGGGAGAATTTCTCCACCGAGATTATTGAGAGCATTTCGCCGGAAGATCTGCAGCCAACTGTTAAGATCTGCATCGACGGCCTCCAGTCGTCGTCAATTGCAGTCAAGCGATCTGCCGCGGCCAAGCTTAGGCTTCTGGCGAAGAACCGGTCCGACAACCGGGTACTCATTGGGGAGTCCGGCGCCGTCCCTGCTCTAATTCCTCTGCTTCGATCCAGCGATCCATGGACGCAAGAGCATGCTGTAACCGCACTGCTAAATCTCTCACTCCATGAGTCCAATAAGGTTATCATCACAAATGCTGGAGCCATAAAATTGCTGGTTTATGCACTCAAAACTGGTACGGAAACTTCGAAGCAGAATGCGGCGTGTGCTCTAATGAGTCTGGCTTTATTGGAGGAAAACAAGACTTCGATTGGGGTTTGCGGGGCCATCCCACCTCTGGTTTCTTTACTATTGAACGGATCGAATAGGGGAAAAAAGGACGCGCTCACAACGCTCTACAAGCTTTGCTCGATCAAGCCTAACAAGGAGCGGGCTGTCTCTGCCGGAGCTGTGAAGCCATTGGTGGCGCTGGTGGTGGAGCAAGGAACGGGTCTGGCAGAGAAGGCCATGGTGGTTCTGAGTAGCCTGGCTGGGATTCAGGAGGGCAAGGATTCGATTGTGGAAGAGGGGGGAATTGCTGCGCTCGTGGAAGCAATCGAAGATGGGTCGGTGAAAGGGAAAGAGTTTGCGGTATTGACACTCTTGCAATTGTGTGTTGAGAGTGTGAGAAATAGAGGGTTGCTCGTAAGGGAAGGCGGAATTCCACCTCTGGTTGCACTTTCGCAGACTGGAAGCGTTCGAGCAAAGCATAAGGCGGAAACACTTCTGGGATATTTAAGAGAACCAAGACAAGAAGCATCCTCATCAAGTCCTTAA

Coding sequence (CDS)

ATGGTGTCGCTTGAGGATTCTCACTCCAGTTCCAATCGGTTTCCTTTGGGGAGAAGTTGCTACAGCCCATCTTCTACCACTTCCAGTAAAATTAGCAGGAACATTGGGCGTTCTATGCGCACTATTCGCTCCAATTTCTTTCAGGATGATAGTAGCTGCACTTTTAACGGTTCGGTAGCCGGAAAATCGGGCTGCGTCTCTGAGAATCTCACCGATTCTGTCATTGACCTTAGGCTTGGCGAGTTGGCATCGCGTAGCCCTACATGGGCCAAGCAATCGTCTGAACAGGAAGAGGATTTTCTTGAACTTTCTCATGCTTTCAGTGATTTCTCTGCTTGTAGCAGTGATATATCAGGCGAGTTGCAGAGGCTTGCAAGTTTGCCGTCCATGGCAGTTGTCCCTAGAAGAGAAGGGGAAGATGCCGAGCCCGAGCCCTGCTTAGGGTTTCTGCAGAGGGAGAATTTCTCCACCGAGATTATTGAGAGCATTTCGCCGGAAGATCTGCAGCCAACTGTTAAGATCTGCATCGACGGCCTCCAGTCGTCGTCAATTGCAGTCAAGCGATCTGCCGCGGCCAAGCTTAGGCTTCTGGCGAAGAACCGGTCCGACAACCGGGTACTCATTGGGGAGTCCGGCGCCGTCCCTGCTCTAATTCCTCTGCTTCGATCCAGCGATCCATGGACGCAAGAGCATGCTGTAACCGCACTGCTAAATCTCTCACTCCATGAGTCCAATAAGGTTATCATCACAAATGCTGGAGCCATAAAATTGCTGGTTTATGCACTCAAAACTGGTACGGAAACTTCGAAGCAGAATGCGGCGTGTGCTCTAATGAGTCTGGCTTTATTGGAGGAAAACAAGACTTCGATTGGGGTTTGCGGGGCCATCCCACCTCTGGTTTCTTTACTATTGAACGGATCGAATAGGGGAAAAAAGGACGCGCTCACAACGCTCTACAAGCTTTGCTCGATCAAGCCTAACAAGGAGCGGGCTGTCTCTGCCGGAGCTGTGAAGCCATTGGTGGCGCTGGTGGTGGAGCAAGGAACGGGTCTGGCAGAGAAGGCCATGGTGGTTCTGAGTAGCCTGGCTGGGATTCAGGAGGGCAAGGATTCGATTGTGGAAGAGGGGGGAATTGCTGCGCTCGTGGAAGCAATCGAAGATGGGTCGGTGAAAGGGAAAGAGTTTGCGGTATTGACACTCTTGCAATTGTGTGTTGAGAGTGTGAGAAATAGAGGGTTGCTCGTAAGGGAAGGCGGAATTCCACCTCTGGTTGCACTTTCGCAGACTGGAAGCGTTCGAGCAAAGCATAAGGCGGAAACACTTCTGGGATATTTAAGAGAACCAAGACAAGAAGCATCCTCATCAAGTCCTTAA

Protein sequence

MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVAGKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGELQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP
Homology
BLAST of Moc01g30220 vs. NCBI nr
Match: XP_022132987.1 (U-box domain-containing protein 4 [Momordica charantia])

HSP 1 Score: 857.8 bits (2215), Expect = 4.2e-245
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA
Sbjct: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQ 180
           LQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQ
Sbjct: 121 LQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQ 180

Query: 181 SSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLS 240
           SSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLS
Sbjct: 181 SSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLS 240

Query: 241 LHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLV 300
           LHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLV
Sbjct: 241 LHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLV 300

Query: 301 SLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLS 360
           SLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLS
Sbjct: 301 SLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLS 360

Query: 361 SLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGI 420
           SLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGI
Sbjct: 361 SLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGI 420

Query: 421 PPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           PPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP
Sbjct: 421 PPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 457

BLAST of Moc01g30220 vs. NCBI nr
Match: KAG6594698.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026666.1 U-box domain-containing protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 824.7 bits (2129), Expect = 3.9e-235
Identity = 438/459 (95.42%), Postives = 448/459 (97.60%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVS+EDSHSSSNRFPLGR+CYSPSSTTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSIEDSHSSSNRFPLGRNCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           GKSGC+SENLTDSVID+RLGELASRSP WA QSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GKSGCISENLTDSVIDIRLGELASRSPKWASQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVV +REG DA  EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVSKREGGDAETEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSS+AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSVAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGAIK LVY LKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAIKSLVYVLKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKER V+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERTVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LSSLAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG
Sbjct: 361 LSSLAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPR EASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRHEASSSSP 459

BLAST of Moc01g30220 vs. NCBI nr
Match: XP_038881710.1 (U-box domain-containing protein 4 [Benincasa hispida])

HSP 1 Score: 824.7 bits (2129), Expect = 3.9e-235
Identity = 438/459 (95.42%), Postives = 450/459 (98.04%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVSLEDSHS+SNRFPL R+CYSPSSTTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSLEDSHSTSNRFPLSRNCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
            KS C+SENLTDSVIDLRLGELASRSP W KQSSEQE+DFLELSHAFSDFSACSSDISGE
Sbjct: 61  AKSSCISENLTDSVIDLRLGELASRSPKWPKQSSEQEQDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVVP+REGEDA  EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVPKREGEDADPEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQ+SSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQASSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGAIK LVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAIKSLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAV+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           L+SLAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVES+RNRGLLVREG
Sbjct: 361 LNSLAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESMRNRGLLVREG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 459

BLAST of Moc01g30220 vs. NCBI nr
Match: XP_023518630.1 (U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823.5 bits (2126), Expect = 8.7e-235
Identity = 438/459 (95.42%), Postives = 448/459 (97.60%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVS+EDSHSSSNRFPLGR+CYSPSSTTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSIEDSHSSSNRFPLGRNCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           G+SGCVSENLTDSVID+RLGELASRSP WA QSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GESGCVSENLTDSVIDIRLGELASRSPKWANQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVV +REG DA  EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVSKREGGDAETEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSS+AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSVAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGAIK LVY LKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAIKSLVYVLKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKER V+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERTVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LSSLAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG
Sbjct: 361 LSSLAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPR EASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRHEASSSSP 459

BLAST of Moc01g30220 vs. NCBI nr
Match: XP_022926504.1 (U-box domain-containing protein 4-like [Cucurbita moschata])

HSP 1 Score: 823.5 bits (2126), Expect = 8.7e-235
Identity = 437/459 (95.21%), Postives = 448/459 (97.60%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVS+EDSHSSSNRFPLGR+CYSP+STTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSIEDSHSSSNRFPLGRNCYSPTSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           GKSGC+SENLTDSVID+RLGELASRSP WA QSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GKSGCISENLTDSVIDIRLGELASRSPKWASQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVV +REG DA  EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVSKREGGDAETEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSS+AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSVAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGAIK LVY LKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAIKSLVYVLKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKER V+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERTVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LSSLAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG
Sbjct: 361 LSSLAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPR EASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRHEASSSSP 459

BLAST of Moc01g30220 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 257.7 bits (657), Expect = 2.5e-67
Identity = 164/385 (42.60%), Postives = 238/385 (61.82%), Query Frame = 0

Query: 85  RSPTWAKQSSEQE---EDFLELSHAFSDFSACSSDISGELQRLASLPSMAVVPRREGEDA 144
           RSP+     S +E    D  E S   +  +  SSD SGE++      + +   RR+  D 
Sbjct: 440 RSPSATSTVSNEEFPRADANENSEESAHATPYSSDASGEIRSGPLAATTSAATRRDLSDF 499

Query: 145 EPEPCLGFLQR-----------ENFSTEIIESISPE------DLQPTVKICIDGLQSSSI 204
            P+    F+ R           E   + I+ + S E      +++  VK  ++ L+SSS+
Sbjct: 500 SPK----FMDRRTRGQFWRRPSERLGSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSL 559

Query: 205 AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLSLHES 264
             +R A A+LRLLAK+  DNR++IG SGA+  L+ LL S+D  TQE+AVTALLNLS++++
Sbjct: 560 DTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQENAVTALLNLSINDN 619

Query: 265 NKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLVSLLL 324
           NK  I +AGAI+ L++ L+ G+  +K+N+A  L SL+++EENK  IG  GAI PLV LL 
Sbjct: 620 NKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLG 679

Query: 325 NGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLSSLAG 384
           NG+ RGKKDA T L+ L   + NK   V +GAV+ L+ L ++   G+ +KA+ VL++LA 
Sbjct: 680 NGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL-MDPAAGMVDKAVAVLANLAT 739

Query: 385 IQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGIPPLV 444
           I EG+++I +EGGI  LVE +E GS +GKE A   LLQL   S R   ++++EG +PPLV
Sbjct: 740 IPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLV 799

Query: 445 ALSQTGSVRAKHKAETLLGYLREPR 450
           ALSQ+G+ RA+ KA+ LL Y R  R
Sbjct: 800 ALSQSGTPRAREKAQALLSYFRNQR 819

BLAST of Moc01g30220 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 8.3e-63
Identity = 142/291 (48.80%), Postives = 197/291 (67.70%), Query Frame = 0

Query: 161 ESISPEDLQPTVKICIDGLQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPL 220
           E+ S   ++  VK  ID L+SSS+  +R A A++R+LA+N +DNR++I    A+P+L+ L
Sbjct: 412 ETGSSSSIETEVKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSL 471

Query: 221 LRSSDPWTQEHAVTALLNLSLHESNKVIITNAGAIKLLVYALKTG-TETSKQNAACALMS 280
           L S+D   Q  AVT LLNLS++++NK +I  +GAI  L++ LKTG  E +K N+A  L S
Sbjct: 472 LYSTDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFS 531

Query: 281 LALLEENKTSIGVCGAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKP 340
           L+++EE KT IG  GAI PLV LL +GS  GKKDA T L+ L     NK + + AGAV+ 
Sbjct: 532 LSVIEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRY 591

Query: 341 LVALVVEQGTGLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLT 400
           LV L ++   G+ EKA+VVL++LA ++EGK +I EEGGI  LVE +E GS +GKE A   
Sbjct: 592 LVEL-MDPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAA 651

Query: 401 LLQLCVESVRNRGLLVREGGIPPLVALSQTGSVRAKHKAETLLGYLREPRQ 451
           LLQLC  S +    ++REG IPPLVAL+++G+ R K KA+ LL Y +  RQ
Sbjct: 652 LLQLCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKAHRQ 701

BLAST of Moc01g30220 vs. ExPASy Swiss-Prot
Match: Q8GWV5 (U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 9.9e-56
Identity = 134/280 (47.86%), Postives = 187/280 (66.79%), Query Frame = 0

Query: 171 TVKICIDGLQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQE 230
           T+K+ ++ L+S S  VK +AAA++R L  N  +NRV IG  GA+  L+ LL S +  TQE
Sbjct: 474 TIKL-VEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQE 533

Query: 231 HAVTALLNLSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSI 290
           HAVTALLNLS+ E NK +I   GAI+ LV+ L TG + +K+N+A +L SL++L+ N+  I
Sbjct: 534 HAVTALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERI 593

Query: 291 GVC-GAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGT 350
           G    AI  LV+LL  G+ RGKKDA + L+ L     NK R V A AVK LV L ++   
Sbjct: 594 GQSNAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVEL-LDPDL 653

Query: 351 GLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVR 410
            + +KA+ +L++L+ + EG+ +IV EGGI  LVE ++ GS +GKE A   LLQLC+ S +
Sbjct: 654 EMVDKAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPK 713

Query: 411 NRGLLVREGGIPPLVALSQTGSVRAKHKAETLLGYLREPR 450
              L+++EG IPPLVALSQ+G+ RAK KA+ LL + R  R
Sbjct: 714 FCTLVLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQR 751

BLAST of Moc01g30220 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.4e-54
Identity = 128/302 (42.38%), Postives = 186/302 (61.59%), Query Frame = 0

Query: 156 STEIIESISPEDLQPTVKICIDGLQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVP 215
           +T+I  S S +  +  V   ++ L + +   +R+AA +LRLLAK   DNRV I E+GA+P
Sbjct: 331 TTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIP 390

Query: 216 ALIPLLRSSDPWTQEHAVTALLNLSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAAC 275
            L+ LL S DP TQEH+VTALLNLS++E NK  I +AGAI  +V  LK G+  +++NAA 
Sbjct: 391 LLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAA 450

Query: 276 ALMSLALLEENKTSIGVCGAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAG 335
            L SL++++ENK +IG  GAI  L+SLL  G+ RGKKDA T ++ LC  + NK RAV  G
Sbjct: 451 TLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGG 510

Query: 336 AVKPLVALVVEQGTGLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEF 395
            V PL  L+ + G G+ ++A+ +L+ L+  QEGK +I E   I  LVE I  GS + +E 
Sbjct: 511 IVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIRTGSPRNREN 570

Query: 396 AVLTLLQLCVESVRNRGLLVREGGIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSS 455
           A   L  LC+ ++    +    G    L  L++ G+ RAK KA +LL  +++    A ++
Sbjct: 571 AAAILWYLCIGNIERLNVAREVGADVALKELTENGTDRAKRKAASLLELIQQTEGVAVTT 630

Query: 456 SP 458
            P
Sbjct: 631 VP 632

BLAST of Moc01g30220 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.7e-53
Identity = 124/274 (45.26%), Postives = 175/274 (63.87%), Query Frame = 0

Query: 179 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 238
           L+S +   +R+AA ++RLLAK   +NR+ I E+GA+P L+ LL SSDP TQEHAVTALLN
Sbjct: 332 LRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVTALLN 391

Query: 239 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 298
           LS+HE+NK  I ++ AI  +V  LKTG+  +++NAA  L SL++++ENK +IG  GAIPP
Sbjct: 392 LSIHENNKASIVDSHAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPP 451

Query: 299 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 358
           L++LL +GS RGKKDA T ++ LC  + NK RAV AG V  L+  +V+   G+ ++A+ +
Sbjct: 452 LINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSL 511

Query: 359 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 418
           LS LAG  EGK  I     I  LVE I+ GS + +E A   L  LC             G
Sbjct: 512 LSILAGNPEGKIVIARSEPIPPLVEVIKTGSPRNRENAAAILWLLCSADTEQTLAAKAAG 571

Query: 419 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEA 453
               L  LS+TG+ RAK KA ++L  + +  +++
Sbjct: 572 VEDALKELSETGTDRAKRKASSILELMHQANEDS 605

BLAST of Moc01g30220 vs. ExPASy TrEMBL
Match: A0A6J1BVC8 (U-box domain-containing protein 4 OS=Momordica charantia OX=3673 GN=LOC111005693 PE=4 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.0e-245
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA
Sbjct: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQ 180
           LQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQ
Sbjct: 121 LQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDGLQ 180

Query: 181 SSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLS 240
           SSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLS
Sbjct: 181 SSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLS 240

Query: 241 LHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLV 300
           LHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLV
Sbjct: 241 LHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLV 300

Query: 301 SLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLS 360
           SLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLS
Sbjct: 301 SLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLS 360

Query: 361 SLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGI 420
           SLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGI
Sbjct: 361 SLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGI 420

Query: 421 PPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           PPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP
Sbjct: 421 PPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 457

BLAST of Moc01g30220 vs. ExPASy TrEMBL
Match: A0A6J1EFA6 (U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111433635 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.2e-235
Identity = 437/459 (95.21%), Postives = 448/459 (97.60%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVS+EDSHSSSNRFPLGR+CYSP+STTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSIEDSHSSSNRFPLGRNCYSPTSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           GKSGC+SENLTDSVID+RLGELASRSP WA QSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GKSGCISENLTDSVIDIRLGELASRSPKWASQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVV +REG DA  EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVSKREGGDAETEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSS+AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSVAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGAIK LVY LKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAIKSLVYVLKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKER V+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERTVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LSSLAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG
Sbjct: 361 LSSLAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPR EASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRHEASSSSP 459

BLAST of Moc01g30220 vs. ExPASy TrEMBL
Match: A0A0A0KL34 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511670 PE=4 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 9.4e-235
Identity = 438/459 (95.42%), Postives = 449/459 (97.82%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVSLEDSHS+SNRFPL R+CYSPSSTTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSLEDSHSTSNRFPLTRNCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
            KSGCVSENLTDSVIDLRLGELASRSP W+KQSSEQE+DFLELSHAFSDFSACSSDISGE
Sbjct: 61  AKSGCVSENLTDSVIDLRLGELASRSPKWSKQSSEQEQDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVVP+REGED   EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVPQREGEDGDPEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGA+K LVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAVKSLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAV+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LSSLAGIQEGKD+IVEEGGIAALVEAIEDGS+KGKEFAVLTLLQLCVESVRNRGLLV EG
Sbjct: 361 LSSLAGIQEGKDAIVEEGGIAALVEAIEDGSLKGKEFAVLTLLQLCVESVRNRGLLVSEG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPRQ ASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQVASSSSP 459

BLAST of Moc01g30220 vs. ExPASy TrEMBL
Match: A0A6J1KQR1 (U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111496781 PE=4 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.6e-234
Identity = 437/459 (95.21%), Postives = 447/459 (97.39%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVS+EDSHSSSNRFPLGR+CYSPSSTTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSIEDSHSSSNRFPLGRNCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
           GKSGCVSENLTDSVID+RLGELASRSP WA QSSEQEEDFLELSHAFSDFSACSSDISGE
Sbjct: 61  GKSGCVSENLTDSVIDIRLGELASRSPKWASQSSEQEEDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVV +REG DA  EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVSKREGGDAETEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSS+AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSVAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGAIK LVY LKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAIKSLVYVLKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKER V+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERTVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LS LAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCV+SVRNRGLLVREG
Sbjct: 361 LSILAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVDSVRNRGLLVREG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPR EASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRHEASSSSP 459

BLAST of Moc01g30220 vs. ExPASy TrEMBL
Match: A0A5D3CRG4 (U-box domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005940 PE=4 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 4.7e-234
Identity = 438/459 (95.42%), Postives = 448/459 (97.60%), Query Frame = 0

Query: 1   MVSLEDSHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDSSCTFNGSVA 60
           MVSLEDSHS+SNRFPL R+CYSPSSTTSSKISRNIGRSMRTIRSNFFQDD+SCTFNGSVA
Sbjct: 1   MVSLEDSHSTSNRFPLTRNCYSPSSTTSSKISRNIGRSMRTIRSNFFQDDNSCTFNGSVA 60

Query: 61  GKSGCVSENLTDSVIDLRLGELASRSPTWAKQSSEQEEDFLELSHAFSDFSACSSDISGE 120
            KS CVSENLTDSVIDLRLGELASRSP W+KQSSEQE+DFLELSHAFSDFSACSSDISGE
Sbjct: 61  AKSVCVSENLTDSVIDLRLGELASRSPKWSKQSSEQEQDFLELSHAFSDFSACSSDISGE 120

Query: 121 LQRLASLPSMAVVPRREGEDA--EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180
           LQRLASLPSMAVVP+REGED   EPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG
Sbjct: 121 LQRLASLPSMAVVPQREGEDGDPEPEPCLGFLQRENFSTEIIESISPEDLQPTVKICIDG 180

Query: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLN 240
           LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRS+DPWTQEHAVTALLN
Sbjct: 181 LQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSTDPWTQEHAVTALLN 240

Query: 241 LSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300
           LSLHESNKVIITNAGA+K LVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP
Sbjct: 241 LSLHESNKVIITNAGAVKSLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPP 300

Query: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVV 360
           LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAV+AGAVKPLVALV EQGTGLAEKAMVV
Sbjct: 301 LVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVTAGAVKPLVALVAEQGTGLAEKAMVV 360

Query: 361 LSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREG 420
           LSSLAGIQEGKD+IVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLV EG
Sbjct: 361 LSSLAGIQEGKDAIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVSEG 420

Query: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQEASSSSP 458
           GIPPLVALSQTGSVRAKHKAETLLGYLREPRQ ASSSSP
Sbjct: 421 GIPPLVALSQTGSVRAKHKAETLLGYLREPRQVASSSSP 459

BLAST of Moc01g30220 vs. TAIR 10
Match: AT4G16490.1 (ARM repeat superfamily protein )

HSP 1 Score: 627.9 bits (1618), Expect = 6.5e-180
Identity = 349/472 (73.94%), Postives = 393/472 (83.26%), Query Frame = 0

Query: 1   MVSLED--SHSSSNRFPLGRSCYSPSSTTSSKISRNIGRSMRTIRSNFFQD-DSSCTFNG 60
           MVS+E+  SHS+S RFPL    Y  SS +++++ R  GRSMRT+RSNF+Q  D SC+F G
Sbjct: 1   MVSVEEPLSHSNSTRFPLTTDFYGSSSPSAARLHRQAGRSMRTVRSNFYQSGDQSCSFVG 60

Query: 61  SVAGKSGCVSENLTDSVIDLRLGELASRSPTW--AKQSSEQEEDFLELSHAFSDFSACSS 120
           S+  KS   SE L+DSVID+RLGELA ++     +  SS +EE FL++S AFSDFSACSS
Sbjct: 61  SIGDKSEYASEFLSDSVIDMRLGELALKNSNSLNSNASSMKEEAFLDISQAFSDFSACSS 120

Query: 121 DISGELQRLASLPSMAVVPRREGED--------AEPEPCLGFLQRENFSTEIIESISPED 180
           DISGELQRLA LPS        G D         E EPCLGFLQRENFSTEIIE ISPED
Sbjct: 121 DISGELQRLACLPSPEADRNESGGDNEAEHDPELEREPCLGFLQRENFSTEIIECISPED 180

Query: 181 LQPTVKICIDGLQSSSIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPW 240
           LQPTVK+CIDGL+SSS+A+KRSAAAKLRLLAKNR+DNRVLIGESGA+ ALIPLLR +DPW
Sbjct: 181 LQPTVKLCIDGLRSSSVAIKRSAAAKLRLLAKNRADNRVLIGESGAIQALIPLLRCNDPW 240

Query: 241 TQEHAVTALLNLSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENK 300
           TQEHAVTALLNLSLH+ NK +I   GAIK LV+ LKTGTETSKQNAACAL+SLALLEENK
Sbjct: 241 TQEHAVTALLNLSLHDQNKAVIAAGGAIKSLVWVLKTGTETSKQNAACALLSLALLEENK 300

Query: 301 TSIGVCGAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQ 360
            SIG CGAIPPLVSLLLNGS RGKKDALTTLYKLC+++ NKERAV+AGAVKPLV LV E+
Sbjct: 301 GSIGACGAIPPLVSLLLNGSCRGKKDALTTLYKLCTLQQNKERAVTAGAVKPLVDLVAEE 360

Query: 361 GTGLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVES 420
           GTG+AEKAMVVLSSLA I +GK++IVEEGGIAALVEAIEDGSVKGKEFA+LTLLQLC +S
Sbjct: 361 GTGMAEKAMVVLSSLAAIDDGKEAIVEEGGIAALVEAIEDGSVKGKEFAILTLLQLCSDS 420

Query: 421 VRNRGLLVREGGIPPLVALSQTG--SVRAKHKAETLLGYLREPRQEASSSSP 458
           VRNRGLLVREG IPPLV LSQ+G  SVRAK KAE LLGYLREPR+EASSSSP
Sbjct: 421 VRNRGLLVREGAIPPLVGLSQSGSVSVRAKRKAERLLGYLREPRKEASSSSP 472

BLAST of Moc01g30220 vs. TAIR 10
Match: AT3G01400.1 (ARM repeat superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.5e-70
Identity = 164/350 (46.86%), Postives = 217/350 (62.00%), Query Frame = 0

Query: 101 LELSHAFSDFSACSSDISGELQRLASLPSMAVVPRREGEDAEPEPCLGFLQRENFSTEII 160
           L L+   S FS C+SD SGE                            F    + S  ++
Sbjct: 21  LSLNDDSSAFSDCNSDRSGE----------------------------FPTASSESRRLL 80

Query: 161 ESISPEDLQPTVKICIDGLQSS-SIAVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIP 220
            S + E+    +   +  L SS SI  ++ AA ++RLL+KN+ +NR+ I ++GA+  LI 
Sbjct: 81  LSCASENSDDLINHLVSHLDSSYSIDEQKQAAMEIRLLSKNKPENRIKIAKAGAIKPLIS 140

Query: 221 LLRSSDPWTQEHAVTALLNLSLHESNKVIITNAGAIKLLVYALKTGTETSKQNAACALMS 280
           L+ SSD   QE+ VTA+LNLSL + NK  I ++GAIK LV ALK GT T+K+NAACAL+ 
Sbjct: 141 LISSSDLQLQEYGVTAILNLSLCDENKESIASSGAIKPLVRALKMGTPTAKENAACALLR 200

Query: 281 LALLEENKTSIGVCGAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKP 340
           L+ +EENK +IG  GAIP LV+LL  G  R KKDA T LY LCS K NK RAV +G +KP
Sbjct: 201 LSQIEENKVAIGRSGAIPLLVNLLETGGFRAKKDASTALYSLCSAKENKIRAVQSGIMKP 260

Query: 341 LVALVVEQGTGLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLT 400
           LV L+ + G+ + +K+  V+S L  + E K +IVEEGG+  LVE +E G+ + KE AV  
Sbjct: 261 LVELMADFGSNMVDKSAFVMSLLMSVPESKPAIVEEGGVPVLVEIVEVGTQRQKEMAVSI 320

Query: 401 LLQLCVESVRNRGLLVREGGIPPLVALSQTGSVRAKHKAETLLGYLREPR 450
           LLQLC ESV  R ++ REG IPPLVALSQ G+ RAK KAE L+  LR+PR
Sbjct: 321 LLQLCEESVVYRTMVAREGAIPPLVALSQAGTSRAKQKAEALIELLRQPR 342

BLAST of Moc01g30220 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 164/385 (42.60%), Postives = 238/385 (61.82%), Query Frame = 0

Query: 85  RSPTWAKQSSEQE---EDFLELSHAFSDFSACSSDISGELQRLASLPSMAVVPRREGEDA 144
           RSP+     S +E    D  E S   +  +  SSD SGE++      + +   RR+  D 
Sbjct: 443 RSPSATSTVSNEEFPRADANENSEESAHATPYSSDASGEIRSGPLAATTSAATRRDLSDF 502

Query: 145 EPEPCLGFLQR-----------ENFSTEIIESISPE------DLQPTVKICIDGLQSSSI 204
            P+    F+ R           E   + I+ + S E      +++  VK  ++ L+SSS+
Sbjct: 503 SPK----FMDRRTRGQFWRRPSERLGSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSL 562

Query: 205 AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLSLHES 264
             +R A A+LRLLAK+  DNR++IG SGA+  L+ LL S+D  TQE+AVTALLNLS++++
Sbjct: 563 DTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQENAVTALLNLSINDN 622

Query: 265 NKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLVSLLL 324
           NK  I +AGAI+ L++ L+ G+  +K+N+A  L SL+++EENK  IG  GAI PLV LL 
Sbjct: 623 NKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLG 682

Query: 325 NGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLSSLAG 384
           NG+ RGKKDA T L+ L   + NK   V +GAV+ L+ L ++   G+ +KA+ VL++LA 
Sbjct: 683 NGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL-MDPAAGMVDKAVAVLANLAT 742

Query: 385 IQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGIPPLV 444
           I EG+++I +EGGI  LVE +E GS +GKE A   LLQL   S R   ++++EG +PPLV
Sbjct: 743 IPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLV 802

Query: 445 ALSQTGSVRAKHKAETLLGYLREPR 450
           ALSQ+G+ RA+ KA+ LL Y R  R
Sbjct: 803 ALSQSGTPRAREKAQALLSYFRNQR 822

BLAST of Moc01g30220 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 164/385 (42.60%), Postives = 238/385 (61.82%), Query Frame = 0

Query: 85  RSPTWAKQSSEQE---EDFLELSHAFSDFSACSSDISGELQRLASLPSMAVVPRREGEDA 144
           RSP+     S +E    D  E S   +  +  SSD SGE++      + +   RR+  D 
Sbjct: 440 RSPSATSTVSNEEFPRADANENSEESAHATPYSSDASGEIRSGPLAATTSAATRRDLSDF 499

Query: 145 EPEPCLGFLQR-----------ENFSTEIIESISPE------DLQPTVKICIDGLQSSSI 204
            P+    F+ R           E   + I+ + S E      +++  VK  ++ L+SSS+
Sbjct: 500 SPK----FMDRRTRGQFWRRPSERLGSRIVSAPSNETRRDLSEVETQVKKLVEELKSSSL 559

Query: 205 AVKRSAAAKLRLLAKNRSDNRVLIGESGAVPALIPLLRSSDPWTQEHAVTALLNLSLHES 264
             +R A A+LRLLAK+  DNR++IG SGA+  L+ LL S+D  TQE+AVTALLNLS++++
Sbjct: 560 DTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQENAVTALLNLSINDN 619

Query: 265 NKVIITNAGAIKLLVYALKTGTETSKQNAACALMSLALLEENKTSIGVCGAIPPLVSLLL 324
           NK  I +AGAI+ L++ L+ G+  +K+N+A  L SL+++EENK  IG  GAI PLV LL 
Sbjct: 620 NKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENKIKIGQSGAIGPLVDLLG 679

Query: 325 NGSNRGKKDALTTLYKLCSIKPNKERAVSAGAVKPLVALVVEQGTGLAEKAMVVLSSLAG 384
           NG+ RGKKDA T L+ L   + NK   V +GAV+ L+ L ++   G+ +KA+ VL++LA 
Sbjct: 680 NGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL-MDPAAGMVDKAVAVLANLAT 739

Query: 385 IQEGKDSIVEEGGIAALVEAIEDGSVKGKEFAVLTLLQLCVESVRNRGLLVREGGIPPLV 444
           I EG+++I +EGGI  LVE +E GS +GKE A   LLQL   S R   ++++EG +PPLV
Sbjct: 740 IPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSGRFCNMVLQEGAVPPLV 799

Query: 445 ALSQTGSVRAKHKAETLLGYLREPR 450
           ALSQ+G+ RA+ KA+ LL Y R  R
Sbjct: 800 ALSQSGTPRAREKAQALLSYFRNQR 819

BLAST of Moc01g30220 vs. TAIR 10
Match: AT5G58680.1 (ARM repeat superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.5e-67
Identity = 157/363 (43.25%), Postives = 223/363 (61.43%), Query Frame = 0

Query: 100 FLELSHAFSD---------FSACSSDISGELQRLASLPSMAVVPRREGEDAEPEPCLGFL 159
           F  + H+FSD         FS C+SDIS E     S                        
Sbjct: 7   FTYMDHSFSDISLNFDSSAFSDCNSDISCEFPTTTS------------------------ 66

Query: 160 QRENFSTEIIESISPEDLQPTVKICIDGLQ-SSSIAVKRSAAAKLRLLAKNRSDNRVLIG 219
             E+   ++  S + ++    ++  I  L+ SSSI  ++ AA ++RLL+KN+ +NR+ + 
Sbjct: 67  --ESRQRKLFLSCAVDNSDDVIRNLITHLESSSSIEEQKQAAMEIRLLSKNKPENRIKLA 126

Query: 220 ESGAVPALIPLLRSSDPWTQEHAVTALLNLSLHESNKVIITNAGAIKLLVYALKTGTETS 279
           ++GA+  L+ L+ SSD   QE+ VTA+LNLSL + NK +I ++GA+K LV AL+ GT T+
Sbjct: 127 KAGAIKPLVSLISSSDLQLQEYGVTAVLNLSLCDENKEMIVSSGAVKPLVNALRLGTPTT 186

Query: 280 KQNAACALMSLALLEENKTSIGVCGAIPPLVSLLLNGSNRGKKDALTTLYKLCSIKPNKE 339
           K+NAACAL+ L+ +EENK +IG  GAIP LV+LL NG  R KKDA T LY LCS   NK 
Sbjct: 187 KENAACALLRLSQVEENKITIGRSGAIPLLVNLLENGGFRAKKDASTALYSLCSTNENKT 246

Query: 340 RAVSAGAVKPLVALVVEQGTGLAEKAMVVLSSLAGIQEGKDSIVEEGGIAALVEAIEDGS 399
           RAV +G +KPLV L+++  + + +K+  V++ L    E K ++VEEGG+  LVE +E G+
Sbjct: 247 RAVESGIMKPLVELMIDFESDMVDKSAFVMNLLMSAPESKPAVVEEGGVPVLVEIVEAGT 306

Query: 400 VKGKEFAVLTLLQLCVESVRNRGLLVREGGIPPLVALSQTGSVR-AKHKAETLLGYLREP 452
            + KE +V  LLQLC ESV  R ++ REG +PPLVALSQ  + R AK KAE L+  LR+P
Sbjct: 307 QRQKEISVSILLQLCEESVVYRTMVAREGAVPPLVALSQGSASRGAKVKAEALIELLRQP 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132987.14.2e-245100.00U-box domain-containing protein 4 [Momordica charantia][more]
KAG6594698.13.9e-23595.42U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038881710.13.9e-23595.42U-box domain-containing protein 4 [Benincasa hispida][more]
XP_023518630.18.7e-23595.42U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo][more]
XP_022926504.18.7e-23595.21U-box domain-containing protein 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O221932.5e-6742.60U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q5XEZ88.3e-6348.80U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 S... [more]
Q8GWV59.9e-5647.86U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 S... [more]
Q8VZ401.4e-5442.38U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q5VRH92.7e-5345.26U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1BVC82.0e-245100.00U-box domain-containing protein 4 OS=Momordica charantia OX=3673 GN=LOC111005693... [more]
A0A6J1EFA64.2e-23595.21U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A0A0KL349.4e-23595.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511670 PE=4 SV=1[more]
A0A6J1KQR11.6e-23495.21U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114967... [more]
A0A5D3CRG44.7e-23495.42U-box domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT4G16490.16.5e-18073.94ARM repeat superfamily protein [more]
AT3G01400.11.5e-7046.86ARM repeat superfamily protein [more]
AT2G23140.11.8e-6842.60RING/U-box superfamily protein with ARM repeat domain [more]
AT2G23140.21.8e-6842.60RING/U-box superfamily protein with ARM repeat domain [more]
AT5G58680.17.5e-6743.25ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 407..447
e-value: 19.0
score: 11.1
coord: 283..323
e-value: 0.83
score: 18.7
coord: 365..405
e-value: 16.0
score: 11.7
coord: 324..364
e-value: 150.0
score: 4.1
coord: 201..241
e-value: 0.0012
score: 28.2
coord: 242..282
e-value: 40.0
score: 8.6
IPR000225ArmadilloPFAMPF00514Armcoord: 202..240
e-value: 2.4E-8
score: 33.7
coord: 285..323
e-value: 9.6E-6
score: 25.5
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 212..254
score: 12.6524
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 335..377
score: 9.2924
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 314..447
e-value: 2.3E-20
score: 75.0
coord: 153..313
e-value: 2.8E-32
score: 114.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 5..453
NoneNo IPR availablePANTHERPTHR23315:SF304U-BOX DOMAIN-CONTAINING PROTEIN 4-LIKEcoord: 5..453
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 169..445

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g30220.1Moc01g30220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding