Moc01g30000 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g30000
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cytochrome P450 CYP82D47-like
Location	chr1: 21213761 .. 21215442 (+)
RNA-Seq Expression	Moc01g30000
Synteny	Moc01g30000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCAAGTCCGCAATTCCATTGGCTCAACCTGATTGCCACTCTTCTGGGCGGTGTCATTGCCATCAATTTCTTACGTTTTCTACACAAAACATTCACCGCTGCCGCCGCGTCAAAGGGTCTAAAGCCGCCCTCAGTCCCCGGCGGGTGGCCTCTGATTGGGCACCTCCCTCTCCTCGGAGGGCCTCAACTGCCACATGTAAAGCTCGGTGCTTTGGCTGATGAATACGGTCCAATCTTCTCCATCCAATTGGGTATCCACCAAGCCGTCGTGATAAACAGCTGGGAGGCGGCGAAACAGTGCTTCACCACCCATGACGTCGCCGTCTCCTTCCGCCCCAAAATTCTCGCCGCTCAAATCTTGGGCTACAACTATGCCGTGTTTGCTTTCGGACCCTACGACTCATACTGGCGCAAAATGCGTAAAACAACCACTCTCCACCTTCTCTCCAGTCGCAGACTCGAGCTTTTGAGAGACGTTCGAGAGTCGGAAGTGAACCAAGCCATAAAAGAGCTATATCAAATTTGGAAAGAAAGAAGAGACAAGCAAGGCCAGGGTTTGGTGGAAATGAAGCAGTGGGTCGGGGACTTGACTATGAACCTCATTCTCGGGGTCGTGGCTGGTAAAAGGTACTTTGGGCCTACAGCAATGGTAGAAGAGGCAGAGGCGCGGGCGTGCCAAAAAGCAATGAAGGAGTTCTTACGCTTAATGGGACTTTTTATACTGGGTGATGCTGTTCCGTATTTAGGATGGTTGGATGTTGGCGGCCATGTGAAGGCCATGAAGAGAACTTCTAAAGAGTTGGACCATATCTTGACACAGTGGTTGGAGGAGCACAAGAAGAAGAGAGCTTCCGGCAAATCCAAAGAAGATAAAGACTTCATGGATGTCATGCTTTCAACTCTCGAGGAAACCCCCGATAATGTTGTTGCTTCAGATTCGATTCATAGCTTCGATGTCGATACCATTATCAAGGCTACATGTTTGGTACGTATTCAACTTTTCAATAAAAAATGATGAGATATAACAAATTATCTGTTTCGACTCTGCTTTTATATGTACAGACCATGGTTTTGGGAGGGAGTGATACAACAACTGTGGTTGTGACGTGGGCGCTCTCATTGCTGCTGAACAATCACCCTGCACTGATAAAAGTGCAAGAAGAAGTCGATGCCCACATCGGCCGAGATAGAGAGTTGAAGGAATCGGACATGAGTGAGCTAGTATATTTGCAGGCCGTAGTGAAGGAGACGATGCGATTGTATGGAGCAGGAGGCCTTATCCTTCGAGAAACCTCAGAGGATTTCGCCATCGACGGGTTCCATATCGAGAAGGGGACAAGACTGTTCATAAACCAGTGGAAGATCCACAGAGATGGCAGGGTGTGGGGAGAGCCACTAGAGTTCAAACCAGAAAGGTTTCTAACGACCCACAGAGATTTTGATGTGAATGGACAGCAGTTTGAGTTGATCCCTTTCGGGGGAGGAAGAAGAGCATGCCCTGGATTGTCTTTTGGACTCCAAATGGTACAACTTATTTTAGGTGCACTGCTTCATGCTTTTGACATATCGAGTGGAGGACGCTGCCGTGGATATGACCGGATCAATTGGATTGCTGAACGTGAAAGCCACTCCATTGGAAGTGTTTCTCAAACCACGCTTACCTCCTGCACTCTATGA

mRNA sequence

ATGGAACCAAGTCCGCAATTCCATTGGCTCAACCTGATTGCCACTCTTCTGGGCGGTGTCATTGCCATCAATTTCTTACGTTTTCTACACAAAACATTCACCGCTGCCGCCGCGTCAAAGGGTCTAAAGCCGCCCTCAGTCCCCGGCGGGTGGCCTCTGATTGGGCACCTCCCTCTCCTCGGAGGGCCTCAACTGCCACATGTAAAGCTCGGTGCTTTGGCTGATGAATACGGTCCAATCTTCTCCATCCAATTGGGTATCCACCAAGCCGTCGTGATAAACAGCTGGGAGGCGGCGAAACAGTGCTTCACCACCCATGACGTCGCCGTCTCCTTCCGCCCCAAAATTCTCGCCGCTCAAATCTTGGGCTACAACTATGCCGTGTTTGCTTTCGGACCCTACGACTCATACTGGCGCAAAATGCGTAAAACAACCACTCTCCACCTTCTCTCCAGTCGCAGACTCGAGCTTTTGAGAGACGTTCGAGAGTCGGAAGTGAACCAAGCCATAAAAGAGCTATATCAAATTTGGAAAGAAAGAAGAGACAAGCAAGGCCAGGGTTTGGTGGAAATGAAGCAGTGGGTCGGGGACTTGACTATGAACCTCATTCTCGGGGTCGTGGCTGGTAAAAGGTACTTTGGGCCTACAGCAATGGTAGAAGAGGCAGAGGCGCGGGCGTGCCAAAAAGCAATGAAGGAGTTCTTACGCTTAATGGGACTTTTTATACTGGGTGATGCTGTTCCGTATTTAGGATGGTTGGATGTTGGCGGCCATGTGAAGGCCATGAAGAGAACTTCTAAAGAGTTGGACCATATCTTGACACAGTGGTTGGAGGAGCACAAGAAGAAGAGAGCTTCCGGCAAATCCAAAGAAGATAAAGACTTCATGGATGTCATGCTTTCAACTCTCGAGGAAACCCCCGATAATGTTGTTGCTTCAGATTCGATTCATAGCTTCGATGTCGATACCATTATCAAGGCTACATGTTTGACCATGGTTTTGGGAGGGAGTGATACAACAACTGTGGTTGTGACGTGGGCGCTCTCATTGCTGCTGAACAATCACCCTGCACTGATAAAAGTGCAAGAAGAAGTCGATGCCCACATCGGCCGAGATAGAGAGTTGAAGGAATCGGACATGAGTGAGCTAGTATATTTGCAGGCCGTAGTGAAGGAGACGATGCGATTGTATGGAGCAGGAGGCCTTATCCTTCGAGAAACCTCAGAGGATTTCGCCATCGACGGGTTCCATATCGAGAAGGGGACAAGACTGTTCATAAACCAGTGGAAGATCCACAGAGATGGCAGGGTGTGGGGAGAGCCACTAGAGTTCAAACCAGAAAGGTTTCTAACGACCCACAGAGATTTTGATGTGAATGGACAGCAGTTTGAGTTGATCCCTTTCGGGGGAGGAAGAAGAGCATGCCCTGGATTGTCTTTTGGACTCCAAATGGTACAACTTATTTTAGGTGCACTGCTTCATGCTTTTGACATATCGAGTGGAGGACGCTGCCGTGGATATGACCGGATCAATTGGATTGCTGAACGTGAAAGCCACTCCATTGGAAGTGTTTCTCAAACCACGCTTACCTCCTGCACTCTATGA

Coding sequence (CDS)

ATGGAACCAAGTCCGCAATTCCATTGGCTCAACCTGATTGCCACTCTTCTGGGCGGTGTCATTGCCATCAATTTCTTACGTTTTCTACACAAAACATTCACCGCTGCCGCCGCGTCAAAGGGTCTAAAGCCGCCCTCAGTCCCCGGCGGGTGGCCTCTGATTGGGCACCTCCCTCTCCTCGGAGGGCCTCAACTGCCACATGTAAAGCTCGGTGCTTTGGCTGATGAATACGGTCCAATCTTCTCCATCCAATTGGGTATCCACCAAGCCGTCGTGATAAACAGCTGGGAGGCGGCGAAACAGTGCTTCACCACCCATGACGTCGCCGTCTCCTTCCGCCCCAAAATTCTCGCCGCTCAAATCTTGGGCTACAACTATGCCGTGTTTGCTTTCGGACCCTACGACTCATACTGGCGCAAAATGCGTAAAACAACCACTCTCCACCTTCTCTCCAGTCGCAGACTCGAGCTTTTGAGAGACGTTCGAGAGTCGGAAGTGAACCAAGCCATAAAAGAGCTATATCAAATTTGGAAAGAAAGAAGAGACAAGCAAGGCCAGGGTTTGGTGGAAATGAAGCAGTGGGTCGGGGACTTGACTATGAACCTCATTCTCGGGGTCGTGGCTGGTAAAAGGTACTTTGGGCCTACAGCAATGGTAGAAGAGGCAGAGGCGCGGGCGTGCCAAAAAGCAATGAAGGAGTTCTTACGCTTAATGGGACTTTTTATACTGGGTGATGCTGTTCCGTATTTAGGATGGTTGGATGTTGGCGGCCATGTGAAGGCCATGAAGAGAACTTCTAAAGAGTTGGACCATATCTTGACACAGTGGTTGGAGGAGCACAAGAAGAAGAGAGCTTCCGGCAAATCCAAAGAAGATAAAGACTTCATGGATGTCATGCTTTCAACTCTCGAGGAAACCCCCGATAATGTTGTTGCTTCAGATTCGATTCATAGCTTCGATGTCGATACCATTATCAAGGCTACATGTTTGACCATGGTTTTGGGAGGGAGTGATACAACAACTGTGGTTGTGACGTGGGCGCTCTCATTGCTGCTGAACAATCACCCTGCACTGATAAAAGTGCAAGAAGAAGTCGATGCCCACATCGGCCGAGATAGAGAGTTGAAGGAATCGGACATGAGTGAGCTAGTATATTTGCAGGCCGTAGTGAAGGAGACGATGCGATTGTATGGAGCAGGAGGCCTTATCCTTCGAGAAACCTCAGAGGATTTCGCCATCGACGGGTTCCATATCGAGAAGGGGACAAGACTGTTCATAAACCAGTGGAAGATCCACAGAGATGGCAGGGTGTGGGGAGAGCCACTAGAGTTCAAACCAGAAAGGTTTCTAACGACCCACAGAGATTTTGATGTGAATGGACAGCAGTTTGAGTTGATCCCTTTCGGGGGAGGAAGAAGAGCATGCCCTGGATTGTCTTTTGGACTCCAAATGGTACAACTTATTTTAGGTGCACTGCTTCATGCTTTTGACATATCGAGTGGAGGACGCTGCCGTGGATATGACCGGATCAATTGGATTGCTGAACGTGAAAGCCACTCCATTGGAAGTGTTTCTCAAACCACGCTTACCTCCTGCACTCTATGA

Protein sequence

MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL

Homology

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BLAST of Moc01g30000 vs. NCBI nr
Match: XP_022133138.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 1   MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60
           MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL
Sbjct: 1   MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60

Query: 61  GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120
           GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ
Sbjct: 61  GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120

Query: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180
           ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER
Sbjct: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180

Query: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240
           RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL
Sbjct: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240

Query: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300
           FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML
Sbjct: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300

Query: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360
           STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK
Sbjct: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360

Query: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420
           VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK
Sbjct: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420

Query: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF 480
           GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF
Sbjct: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF 480

Query: 481 GLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL 535
           GLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL
Sbjct: 481 GLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL 534

[Close]

BLAST of Moc01g30000 vs. NCBI nr
Match: XP_022132489.1 (cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 792.7 bits (2046), Expect = 1.9e-225
Identity = 395/469 (84.22%), Postives = 422/469 (89.98%), Query Frame = 0


Query: 1   MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60
           MEP+ QF+WL+LI TLL GVIAINF RFL KTFT   ASKGLKPP+V GGWPLIGHLPLL
Sbjct: 1   MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFT---ASKGLKPPTVAGGWPLIGHLPLL 60

Query: 61  GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120
            GP+LPH+KL ALA+EYGPIFSIQLGI +AVVINSWEAAKQCFTTHDVA+S RP ILA++
Sbjct: 61  AGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASK 120

Query: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180
           ILGYNYA+FAFGPYDSYWRKMRKTT LHLLS+RRLELLR+VRESEVNQAIKELY  WKER
Sbjct: 121 ILGYNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKER 180

Query: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240
           RD+QGQ LVEMKQWVGDLTMNLILGVVAGKRYFG  AMV EAEARAC +A KEFLRLMGL
Sbjct: 181 RDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGL 240

Query: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300
           F LGD VPYLGWLDVGGHVKAMKRTSKELDH+LT+WLEEH+K RASG+SKEDKDFMDVML
Sbjct: 241 FTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVML 300

Query: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360
           S LEE   NVV SDS HSFD DTIIKATCLTMVLGGSDTT VVVTWALSLLLNN PAL K
Sbjct: 301 SVLEEETANVV-SDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRK 360

Query: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420
           VQ+E+DA IGRDREL ESD+ +LVY QAVVKETMRLYG  GL +RETSEDFAIDGFHIEK
Sbjct: 361 VQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEK 420

Query: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFG 470
           GT LF+N WKIHRD RVWGEPLEFKPERFLTTH+DFDV GQQFELIPFG
Sbjct: 421 GTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG 465

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BLAST of Moc01g30000 vs. NCBI nr
Match: XP_038883369.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-184
Identity = 339/507 (66.86%), Postives = 392/507 (77.32%), Query Frame = 0


Query: 1   MEPSPQFHWLNLIATLLGG---VIAINFLRFLHKTFT----AAAASKGLKPPSVPGGWPL 60
           +E   +  W N   T+L G   ++AI   R + K  T         +  KPP V GGWPL
Sbjct: 3   VEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPL 62

Query: 61  IGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFR 120
           I HLPL GGP+ PH+KL ALAD+YGPIFSIQLG H AVVIN+WEAAKQCFTTHD+AV  R
Sbjct: 63  IRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPR 122

Query: 121 PKILAAQILGYNYAVFAFGP-YDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKE 180
           PK+L+ +++GYNYA+F F P Y+SYWRKMRK TTL LLS+RRL+LLR VRESEVN+AIKE
Sbjct: 123 PKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKE 182

Query: 181 LYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMK 240
           LYQ+          G VEMKQW GDL MNL+L +V GKRYFG  AMVEE EA+ CQKAM+
Sbjct: 183 LYQL---------GGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAME 242

Query: 241 EFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKED 300
           E LRL+G+++LGDA+PYLGWLDVGGHVKAMKRT K LD +LTQWLEEHKK RA    K +
Sbjct: 243 ESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA---GKNN 302

Query: 301 KDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLL 360
            DFMDVMLS LEET DN    D +H F  DT+IKATCLTMVLG S+T+ V V WALSLLL
Sbjct: 303 GDFMDVMLSVLEET-DN----DILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLL 362

Query: 361 NNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFA 420
           NN   L KVQEE+D HIGRD +L+ESDM +LVYL+AVVKE MRL+GAG LI RE +ED  
Sbjct: 363 NNRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDID 422

Query: 421 IDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDV-NGQQFELIPFGGG 480
           +DGFHI+KGT LFIN WKIHRD RVW EPL+FKPERFLTTH++FDV  GQQFELIPFGGG
Sbjct: 423 VDGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGG 482

Query: 481 RRACPGLSFGLQMVQLILGALLHAFDI 499
           RR CPGL FGLQ+V+ +LG LLHAFDI
Sbjct: 483 RRICPGLWFGLQLVEFVLGGLLHAFDI 492

[Close]

BLAST of Moc01g30000 vs. NCBI nr
Match: XP_022133139.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 656.0 bits (1691), Expect = 2.8e-184
Identity = 329/373 (88.20%), Postives = 346/373 (92.76%), Query Frame = 0


Query: 128 VFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG 187
           +F FGPY+SYWRKMRKTT +HLLS+RRLELLR+VRESEV+QAIKELYQIWK+RRD QG+G
Sbjct: 1   MFGFGPYNSYWRKMRKTTNVHLLSNRRLELLRNVRESEVSQAIKELYQIWKKRRDGQGRG 60

Query: 188 LVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAV 247
           LVEMKQW+G+LTMNLILGVVA KRYFGP AMVEE EARACQKA KEFLRLMGLF LGDA 
Sbjct: 61  LVEMKQWIGELTMNLILGVVASKRYFGPAAMVEEDEARACQKATKEFLRLMGLFTLGDAA 120

Query: 248 PYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETP 307
           P+LGWLDVGGHVKAMKRTSKELDHILTQWLEEHKK RASG SKEDKDFMDVMLSTLEETP
Sbjct: 121 PHLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKDRASGGSKEDKDFMDVMLSTLEETP 180

Query: 308 DNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDA 367
           D VV SDSIHSFDVDTIIKATCLTMVLGG+DTT VVVTW LSLLLNN PAL KVQEE+DA
Sbjct: 181 DAVV-SDSIHSFDVDTIIKATCLTMVLGGNDTTVVVVTWTLSLLLNNRPALRKVQEELDA 240

Query: 368 HIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEKGTRLFIN 427
           HIGR+RELKESDMSELVYLQAVVKETMRLYGA GLILRETS DFAIDGF IEKGT +FIN
Sbjct: 241 HIGRERELKESDMSELVYLQAVVKETMRLYGAVGLILRETSVDFAIDGFQIEKGTWIFIN 300

Query: 428 QWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQL 487
            WKIHRD RVWGEPLEFKPERFLTTH DFDV GQQFELIPFGGGRRAC G+SFGLQMVQL
Sbjct: 301 LWKIHRDERVWGEPLEFKPERFLTTHIDFDVKGQQFELIPFGGGRRACSGISFGLQMVQL 360

Query: 488 ILGALLHAFDISS 501
           ILG LLHAFDIS+
Sbjct: 361 ILGTLLHAFDIST 372

[Close]

BLAST of Moc01g30000 vs. NCBI nr
Match: KAG2663852.1 (hypothetical protein I3760_16G051800 [Carya illinoinensis])

HSP 1 Score: 592.8 bits (1527), Expect = 2.9e-165
Identity = 290/493 (58.82%), Postives = 369/493 (74.85%), Query Frame = 0


Query: 15  TLLGGVIAINFLR-FLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGG-PQLPHVKLGA 74
           T++ G+ +I  L  F+ K F    ASK  KPP   GGWPLIGHL LLGG  QLPH+ LG+
Sbjct: 10  TIIAGLFSIIILSYFIQKRF---GASKSRKPPEAAGGWPLIGHLHLLGGKSQLPHIVLGS 69

Query: 75  LADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFG 134
           LAD+YG +FSI++G+H A+VI+SWE AK+CFTT+DVA++ RPK+  A+ LGY+Y +  F 
Sbjct: 70  LADKYGSVFSIRIGVHSALVISSWELAKECFTTNDVALASRPKLAGAKYLGYDYVMIGFS 129

Query: 135 PYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG----L 194
           PY SYWR+MRK  +L LLS+RRLELL+DVR SE+  ++KELY++W E++D    G     
Sbjct: 130 PYGSYWREMRKIISLELLSNRRLELLKDVRASEMETSLKELYKVWTEKKDGSDGGGGGVS 189

Query: 195 VEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAVP 254
           VEMKQW GDL +N++L +VAGKRYFG  A  ++ EAR CQKA +EF  LMG F++GDA+P
Sbjct: 190 VEMKQWFGDLALNVVLRMVAGKRYFGAAATADK-EARRCQKAFREFFHLMGQFLVGDAIP 249

Query: 255 YLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETPD 314
           YLGW+D+GGHVKAMK+T+KE+D + T+WLEEH+K R SG+SK+ +DFMD+MLS LE    
Sbjct: 250 YLGWMDLGGHVKAMKKTAKEMDCMATEWLEEHRKNRVSGESKQQQDFMDIMLSVLE---- 309

Query: 315 NVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAH 374
                  +  FD DT+ K+ CL M+ GG+DTT V +TW LSLLLNN  ALIK QEE+D H
Sbjct: 310 ----GADLGDFDADTVNKSACLNMISGGTDTTMVSLTWTLSLLLNNRHALIKAQEELDMH 369

Query: 375 IGRDRELKESDMSELVYLQAVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFIN 434
           +GR R + E D+S+L YLQA+VKE +RLY  G L   RE +ED  I GFH+ KGTR+  N
Sbjct: 370 VGRGRLVNEGDISKLFYLQAIVKEALRLYPPGPLSGPREAAEDCTIGGFHVAKGTRVVFN 429

Query: 435 QWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQL 494
            WKI  D ++W EPLEFKPERFLTTH+D DV GQ FELIPFG GRRACPG+SFGLQM+ L
Sbjct: 430 LWKIQTDPKIWSEPLEFKPERFLTTHKDVDVRGQNFELIPFGSGRRACPGISFGLQMLHL 489

Query: 495 ILGALLHAFDISS 501
            L +LLHAF+IS+
Sbjct: 490 TLASLLHAFEIST 490

[Close]

BLAST of Moc01g30000 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 6.0e-137
Identity = 245/492 (49.80%), Postives = 335/492 (68.09%), Query Frame = 0


Query: 10  LNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPHVK 69
           LNL    +  ++++ FL      F     S+G   P V G WP++GHL LL G Q PH  
Sbjct: 8   LNLNTIAIASILSLIFLCL----FLYRKNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKV 67

Query: 70  LGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVF 129
           LGALAD+YGP+F+I+LG+  A+V+++WE +K+ FTT+D+AVS RPK++A +++ YN A  
Sbjct: 68  LGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFV 127

Query: 130 AFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKE-RRDKQGQGL 189
              PY  YWR++RK  T   LS+RR+E    +R SEV  +IKEL+ IW    +++    L
Sbjct: 128 GLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTL 187

Query: 190 VEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAVP 249
           V++KQW+  LT N+++ +V GKRYFG   +  + +A+   K ++EF+ LMG F + D VP
Sbjct: 188 VDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVP 247

Query: 250 YLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKE-DKDFMDVMLSTLEETP 309
            L WLD+GGH KAMK  +KE+D +L++WLEEH++K+  G++ E D+DFMDVM+S L    
Sbjct: 248 CLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALN--- 307

Query: 310 DNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDA 369
                   I +FD DTI KAT L ++LGG+D+T V +TWALSLLL N  AL K +EE+D 
Sbjct: 308 -----GAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDM 367

Query: 370 HIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLIL-RETSEDFAIDGFHIEKGTRLFI 429
            IG+D  ++ESD+S+LVYLQA+VKET+RLY        RE +E+  + G+HI+KGTRL  
Sbjct: 368 QIGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIH 427

Query: 430 NQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQ 489
           N WKIHRD  VW +PLEFKPERFLTTH+D D+ G  FEL+PFG GRR C G+S GL MV 
Sbjct: 428 NLWKIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVH 487

Query: 490 LILGALLHAFDI 499
             L  LLH+FDI
Sbjct: 488 FTLANLLHSFDI 487

[Close]

BLAST of Moc01g30000 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 9.5e-135
Identity = 240/493 (48.68%), Postives = 340/493 (68.97%), Query Frame = 0


Query: 10  LNLIATLLGGVIAINFL--RFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPH 69
           L+ + T   GV+++ FL   FL+        S   + P+V G WP+ GHLPLL G + PH
Sbjct: 5   LHFLNTTTIGVVSLIFLLCLFLYGPLKKVHGS-SKEAPTVGGAWPIFGHLPLLIGSKSPH 64

Query: 70  VKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYA 129
             LGALA+++GP+F+I+LG  +A+V++ WE A++CFTT+DVAVS RPK+L A+++ YN A
Sbjct: 65  KALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNA 124

Query: 130 VFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG 189
           +    PY  YWR++RK     +LSS R+E L+DVR SEV  +I ELY +W+ ++++    
Sbjct: 125 MLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYA 184

Query: 190 LVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAV 249
            VE+KQW      N++L +V GKR+   TA  E+AE   C KA+ EF+RL G+F +GDA+
Sbjct: 185 SVELKQWFAQPIFNMVLRMVVGKRFLSATATDEKAE--KCVKAVDEFMRLAGVFTVGDAI 244

Query: 250 PYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKED-KDFMDVMLSTLEET 309
           PYL WLD GG+ KAMK T+KELD ++++WLEEH++KRA G+  +  +DFM+VMLS+L+  
Sbjct: 245 PYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSLD-- 304

Query: 310 PDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVD 369
                   +I   D DT+IK+T LT++  G++ +   + WA+ L+L N P +++ + E+D
Sbjct: 305 ------GKTIDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKN-PLILENKAELD 364

Query: 370 AHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLIL-RETSEDFAIDGFHIEKGTRLF 429
             +G+DR + ESD+S LVYLQAVVKET+RLY  G L   RE +ED  + G+H++KGTRL 
Sbjct: 365 IQVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLI 424

Query: 430 INQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMV 489
            N WKIH D  VW +P EFKP+RFLTTH+D DV G  F+L+PFG GRR CPG+SFGLQ V
Sbjct: 425 TNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTV 484

Query: 490 QLILGALLHAFDI 499
            L L + LH+F+I
Sbjct: 485 HLALASFLHSFEI 485

[Close]

BLAST of Moc01g30000 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 475.7 bits (1223), Expect = 6.8e-133
Identity = 242/511 (47.36%), Postives = 335/511 (65.56%), Query Frame = 0


Query: 12  LIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPHVKLG 71
           L  T +  +  I+ L FL   F  +  S   +PP + G WPL+GHLPL+   Q PH  LG
Sbjct: 6   LNTTTIAFISLISLLFFL---FRFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLG 65

Query: 72  ALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAF 131
           AL D+YGPIF+I+LG   A+V+++WE AK+CFT +D+ VS RPK +A +++ YN A   +
Sbjct: 66  ALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGW 125

Query: 132 GPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGL--- 191
            PY +YWR++RK  TL +LS+RR+ELL  +R SEV  +IKEL  +W  +   Q  GL   
Sbjct: 126 APYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQ-YGLLDD 185

Query: 192 -----------------VEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAM 251
                            VE+K+W   LT+N++L +V GKR FG   +  + EA+   + +
Sbjct: 186 TKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENI 245

Query: 252 KEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKE 311
           ++F+RL+G F +GD VP+L WLD+GGH K MK+ +K+ D +L +WLEEH++K+  G   +
Sbjct: 246 RDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDK 305

Query: 312 ---DKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWAL 371
              ++DFMD ML  L++ P        I  FDVDTIIKAT L ++LGGSDTT   +TWA+
Sbjct: 306 VVGERDFMDAMLLVLKDKP--------IEGFDVDTIIKATTLELILGGSDTTAGTLTWAM 365

Query: 372 SLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLIL-RET 431
            LLL +   L K++EE++ +IG++R + ESD+++LVYL A++KET+RLY        RE 
Sbjct: 366 CLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREF 425

Query: 432 SEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIP 491
           +ED  I G+HI+KGTRL  N WKIHRD  VW +PLEFKPERFL+TH+D DV GQ FEL+P
Sbjct: 426 TEDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLP 485

Query: 492 FGGGRRACPGLSFGLQMVQLILGALLHAFDI 499
           FG GRR C G+S GL MV  IL   LH+F+I
Sbjct: 486 FGSGRRMCAGMSLGLHMVHYILANFLHSFEI 504

[Close]

BLAST of Moc01g30000 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.4e-130
Identity = 248/495 (50.10%), Postives = 326/495 (65.86%), Query Frame = 0


Query: 10  LNLIATLLGG--VIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPH 69
           L+ + TLL G  V+   F  F+ K       +K  +PP   G WP+IGHL LL    LPH
Sbjct: 4   LHCLETLLLGFFVLLPCFFYFVWK----KPNNKIKEPPQPAGAWPIIGHLHLLARGDLPH 63

Query: 70  VKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYA 129
             L + AD+ GP+F IQLG+HQA+V+N+ E AK+CFTT+D     RP  +AA+I+GYNY 
Sbjct: 64  KILSSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYV 123

Query: 130 VFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG 189
           +    PY  YWR MRK   L  LS+RRL+ L+ V  SE++ + KELY++W+ +       
Sbjct: 124 MLGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQ--NIDFC 183

Query: 190 LVEMKQWVGDLTMNLILGVVAGKRYFG--PTAMVEEAEARACQKAMKEFLRLMGLFILGD 249
           LV+MKQW+ DLT+N+ + +V GKR+FG    +  EE E+  C K ++   RLMG F+L D
Sbjct: 184 LVDMKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSD 243

Query: 250 AVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRAS-GKSKEDKDFMDVMLSTLE 309
            +PYL WLD+GGH K MKRT KELD +   WL+EHK+KR S GK  +D+DFMDVMLS LE
Sbjct: 244 YLPYLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILE 303

Query: 310 ETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEE 369
           E         S    DVDTI K  CL ++LGG+DTT   +TWALSLLLNN  AL K Q+E
Sbjct: 304 E---------SKLGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDE 363

Query: 370 VDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTR 429
           +D H+GRDR + ESD+ +L Y+ A++KET+RLY  G L+  R  +ED  I G+H+  GTR
Sbjct: 364 LDLHVGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTR 423

Query: 430 LFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQ 489
           L +N WKI RD  VW +P E++PERFL   RD D+ GQ FELIPFG GRRACP +S  LQ
Sbjct: 424 LIVNAWKIQRDPLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQ 481

Query: 490 MVQLILGALLHAFDI 499
           ++ L L  +LH F++
Sbjct: 484 VLPLTLAHILHGFEL 481

[Close]

BLAST of Moc01g30000 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.5e-127
Identity = 228/475 (48.00%), Postives = 326/475 (68.63%), Query Frame = 0


Query: 33  FTAAAASKGLKPPSVPGGWPLIGHLPLLGG-PQLPHVKLGALADEYGPIFSIQLGIHQAV 92
           F  +   K +K P+  G WP+IGHL LLGG  QL +  LG +AD YGP  S+QLG ++A 
Sbjct: 21  FKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAF 80

Query: 93  VINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLS 152
           V++S+E AK CFT +D A++ RP   AA+ +GYN+AVF F PY ++WR+MRK  T+ LLS
Sbjct: 81  VVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLS 140

Query: 153 SRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKR 212
           +RRL++L+ VR SE+   +K+LY +W +    +   +V++K W+ D+T+N+I+ +VAGKR
Sbjct: 141 NRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK-PVMVDLKSWLEDMTLNMIVRMVAGKR 200

Query: 213 YFGPTAMV---EEAEARACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKE 272
           YFG    V   +  EA  C+KA+ +F  L+G+F + DA P L + D+ GH K MK+T  E
Sbjct: 201 YFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSE 260

Query: 273 LDHILTQWLEEHKKKRA-SGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKA 332
           LD IL +W+E H+++R  SG  + D DF+DVM+S  E+         S   +D +T IK+
Sbjct: 261 LDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQ------GKLSHLQYDANTSIKS 320

Query: 333 TCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQ 392
           TCL ++LGGSDT+   +TWA+SLLLNN   L K Q+E+D H+GRDR +++SD+  LVYLQ
Sbjct: 321 TCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQ 380

Query: 393 AVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKP 452
           A++KET+RLY AG L+  RE  ED  + G+++  GTRL +N WKI RD +V+ EP EF+P
Sbjct: 381 AIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRP 440

Query: 453 ERFLTTH-RDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDISS 501
           ERF+T   ++FDV GQ FEL+PFG GRR+CPG S  +Q++ L L   LH+FD+ +
Sbjct: 441 ERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKT 488

[Close]

BLAST of Moc01g30000 vs. ExPASy TrEMBL
Match: A0A6J1BY79 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=3 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 1   MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60
           MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL
Sbjct: 1   MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60

Query: 61  GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120
           GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ
Sbjct: 61  GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120

Query: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180
           ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER
Sbjct: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180

Query: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240
           RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL
Sbjct: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240

Query: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300
           FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML
Sbjct: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300

Query: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360
           STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK
Sbjct: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360

Query: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420
           VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK
Sbjct: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420

Query: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF 480
           GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF
Sbjct: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF 480

Query: 481 GLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL 535
           GLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL
Sbjct: 481 GLQMVQLILGALLHAFDISSGGRCRGYDRINWIAERESHSIGSVSQTTLTSCTL 534

[Close]

BLAST of Moc01g30000 vs. ExPASy TrEMBL
Match: A0A6J1BSL4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 9.3e-226
Identity = 395/469 (84.22%), Postives = 422/469 (89.98%), Query Frame = 0


Query: 1   MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60
           MEP+ QF+WL+LI TLL GVIAINF RFL KTFT   ASKGLKPP+V GGWPLIGHLPLL
Sbjct: 1   MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFT---ASKGLKPPTVAGGWPLIGHLPLL 60

Query: 61  GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120
            GP+LPH+KL ALA+EYGPIFSIQLGI +AVVINSWEAAKQCFTTHDVA+S RP ILA++
Sbjct: 61  AGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASK 120

Query: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180
           ILGYNYA+FAFGPYDSYWRKMRKTT LHLLS+RRLELLR+VRESEVNQAIKELY  WKER
Sbjct: 121 ILGYNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKER 180

Query: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240
           RD+QGQ LVEMKQWVGDLTMNLILGVVAGKRYFG  AMV EAEARAC +A KEFLRLMGL
Sbjct: 181 RDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGL 240

Query: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300
           F LGD VPYLGWLDVGGHVKAMKRTSKELDH+LT+WLEEH+K RASG+SKEDKDFMDVML
Sbjct: 241 FTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVML 300

Query: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360
           S LEE   NVV SDS HSFD DTIIKATCLTMVLGGSDTT VVVTWALSLLLNN PAL K
Sbjct: 301 SVLEEETANVV-SDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRK 360

Query: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420
           VQ+E+DA IGRDREL ESD+ +LVY QAVVKETMRLYG  GL +RETSEDFAIDGFHIEK
Sbjct: 361 VQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEK 420

Query: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFG 470
           GT LF+N WKIHRD RVWGEPLEFKPERFLTTH+DFDV GQQFELIPFG
Sbjct: 421 GTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG 465

[Close]

BLAST of Moc01g30000 vs. ExPASy TrEMBL
Match: A0A6J1BV48 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005814 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.4e-184
Identity = 329/373 (88.20%), Postives = 346/373 (92.76%), Query Frame = 0


Query: 128 VFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG 187
           +F FGPY+SYWRKMRKTT +HLLS+RRLELLR+VRESEV+QAIKELYQIWK+RRD QG+G
Sbjct: 1   MFGFGPYNSYWRKMRKTTNVHLLSNRRLELLRNVRESEVSQAIKELYQIWKKRRDGQGRG 60

Query: 188 LVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAV 247
           LVEMKQW+G+LTMNLILGVVA KRYFGP AMVEE EARACQKA KEFLRLMGLF LGDA 
Sbjct: 61  LVEMKQWIGELTMNLILGVVASKRYFGPAAMVEEDEARACQKATKEFLRLMGLFTLGDAA 120

Query: 248 PYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETP 307
           P+LGWLDVGGHVKAMKRTSKELDHILTQWLEEHKK RASG SKEDKDFMDVMLSTLEETP
Sbjct: 121 PHLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKDRASGGSKEDKDFMDVMLSTLEETP 180

Query: 308 DNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDA 367
           D VV SDSIHSFDVDTIIKATCLTMVLGG+DTT VVVTW LSLLLNN PAL KVQEE+DA
Sbjct: 181 DAVV-SDSIHSFDVDTIIKATCLTMVLGGNDTTVVVVTWTLSLLLNNRPALRKVQEELDA 240

Query: 368 HIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEKGTRLFIN 427
           HIGR+RELKESDMSELVYLQAVVKETMRLYGA GLILRETS DFAIDGF IEKGT +FIN
Sbjct: 241 HIGRERELKESDMSELVYLQAVVKETMRLYGAVGLILRETSVDFAIDGFQIEKGTWIFIN 300

Query: 428 QWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQL 487
            WKIHRD RVWGEPLEFKPERFLTTH DFDV GQQFELIPFGGGRRAC G+SFGLQMVQL
Sbjct: 301 LWKIHRDERVWGEPLEFKPERFLTTHIDFDVKGQQFELIPFGGGRRACSGISFGLQMVQL 360

Query: 488 ILGALLHAFDISS 501
           ILG LLHAFDIS+
Sbjct: 361 ILGTLLHAFDIST 372

[Close]

BLAST of Moc01g30000 vs. ExPASy TrEMBL
Match: A0A2I4EQH4 (cytochrome P450 CYP82D47-like OS=Juglans regia OX=51240 GN=LOC108991732 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 7.0e-165
Identity = 289/493 (58.62%), Postives = 372/493 (75.46%), Query Frame = 0


Query: 15  TLLGGVIAINFLR-FLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGG-PQLPHVKLGA 74
           T++ G+ +I  L  F+ K F A+  S+  KPP   GGWPLIGHL LLGG  QLPH+ LG+
Sbjct: 10  TIIAGLFSIIILSYFIQKRFGASMISR--KPPEAAGGWPLIGHLHLLGGKSQLPHIVLGS 69

Query: 75  LADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFG 134
           LA++YG +FSI++G+H AVVI+SWE AK+CFTT+DVA++  PK+  A+ LGY+Y +F F 
Sbjct: 70  LAEKYGSVFSIRIGMHSAVVISSWEMAKECFTTNDVALASHPKLAGAKHLGYDYVMFGFS 129

Query: 135 PYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG----L 194
           PY SYWR++RK  +L LLS+RRLELL+DVR SE+  ++KELY++W E++D    G     
Sbjct: 130 PYGSYWREIRKIISLELLSNRRLELLKDVRASEMETSLKELYKVWTEKKDVSDGGGGGVS 189

Query: 195 VEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAVP 254
           VEMKQW GDLT+N++L +VAGKRYFG  A  ++ EA+ CQKA +EF  LMG F++GDA+P
Sbjct: 190 VEMKQWFGDLTLNVVLRMVAGKRYFGAAAAADK-EAQRCQKAFREFFHLMGQFLVGDAIP 249

Query: 255 YLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETPD 314
           YLGW+D+GGHVKAMK+T+KE+D + T+WLEEH+K R SG+SKE  DF+D+MLS LE    
Sbjct: 250 YLGWMDLGGHVKAMKKTAKEMDCLATEWLEEHRKNRVSGESKEQLDFIDIMLSVLE---- 309

Query: 315 NVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAH 374
                  +  FD DT+ K+TCL M+ GG+DTT V +TWALSLLLNN  ALIK QEE+D H
Sbjct: 310 ----GADLGDFDADTVNKSTCLNMISGGTDTTMVSLTWALSLLLNNRHALIKAQEELDMH 369

Query: 375 IGRDRELKESDMSELVYLQAVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFIN 434
           +GR R + E D+S L YLQA+VKE +RLY  G L   RE +ED  I GFH+ KGTR+ +N
Sbjct: 370 VGRGRLVNEGDISNLFYLQAIVKEALRLYPPGPLSGPREATEDCTIGGFHVAKGTRVLVN 429

Query: 435 QWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQL 494
            WKI  D ++W EPLEFKPERFLTTH+D DV GQ FELIPFG GRRACPG++FGLQM+ L
Sbjct: 430 LWKIQTDPKIWSEPLEFKPERFLTTHKDVDVKGQNFELIPFGSGRRACPGIAFGLQMLHL 489

Query: 495 ILGALLHAFDISS 501
            L +LLHAF+IS+
Sbjct: 490 PLASLLHAFEIST 491

[Close]

BLAST of Moc01g30000 vs. ExPASy TrEMBL
Match: A0A6J5TPS2 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7991 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 3.3e-162
Identity = 289/494 (58.50%), Postives = 366/494 (74.09%), Query Frame = 0


Query: 9   WLNLIATLLGGVIAI-NFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPH 68
           +L  + T + G++AI  F  F+ K  ++AA +KG K P V GGWPL+GH+ L GG QLPH
Sbjct: 4   YLPCLNTAIAGILAILLFSYFIIKRSSSAAKAKGPKLPKVAGGWPLLGHVGLFGGSQLPH 63

Query: 69  VKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYA 128
           + L +L D+YGP F+I +GIH A+VI++WE AK CFTT+D+ VS RP  LAA+ LGYN+A
Sbjct: 64  ITLASLVDKYGPAFTINIGIHSALVISTWEVAKDCFTTNDIVVSSRPPTLAAKHLGYNFA 123

Query: 129 VFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQG 188
           +F F PY  YWR+MRK T+L LLS+RRLELL+ VR SEV  ++KELY +W +R++  G+ 
Sbjct: 124 MFGFSPYGPYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGER 183

Query: 189 LVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAV 248
           LVEMKQW GDLT+N+IL +VAGKR F    + EE EAR  QKAM+EF RL GLF+LGDAV
Sbjct: 184 LVEMKQWFGDLTLNVILRMVAGKRCFMNGNLSEEKEARRWQKAMREFFRLAGLFVLGDAV 243

Query: 249 PYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETP 308
           P+L W D+GG  KAMK+T+KELD I+ +WLEEHK+K   GK   D+DFMDVMLST+  T 
Sbjct: 244 PWLSWFDLGGQQKAMKKTAKELDSIVAEWLEEHKQKITEGK---DQDFMDVMLSTINGTV 303

Query: 309 DNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDA 368
            +V        FD DT+IKATCL ++ GGSDTT V +TWALSLLLNN   L KV EE+D 
Sbjct: 304 TDVA------GFDADTVIKATCLILIAGGSDTTMVTLTWALSLLLNNRQVLKKVYEELDQ 363

Query: 369 HIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFI 428
           ++G+ R L+ESD++ LVYLQA VKE MRL  AG L   RE +ED  + G+H+ KGT L +
Sbjct: 364 YVGKGRLLEESDINNLVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLV 423

Query: 429 NQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQ 488
           N WKI  D RVW +P+EFKPERFLTTH+D DV GQQFEL+PFG GRRACPG+SFGLQM  
Sbjct: 424 NLWKIQTDPRVWADPMEFKPERFLTTHKDVDVRGQQFELMPFGSGRRACPGISFGLQMTL 483

Query: 489 LILGALLHAFDISS 501
           + L + LH FD+++
Sbjct: 484 ITLASFLHWFDVTT 488

[Close]

BLAST of Moc01g30000 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 457.2 bits (1175), Expect = 1.8e-128
Identity = 228/475 (48.00%), Postives = 326/475 (68.63%), Query Frame = 0


Query: 33  FTAAAASKGLKPPSVPGGWPLIGHLPLLGG-PQLPHVKLGALADEYGPIFSIQLGIHQAV 92
           F  +   K +K P+  G WP+IGHL LLGG  QL +  LG +AD YGP  S+QLG ++A 
Sbjct: 21  FKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAF 80

Query: 93  VINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLS 152
           V++S+E AK CFT +D A++ RP   AA+ +GYN+AVF F PY ++WR+MRK  T+ LLS
Sbjct: 81  VVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLS 140

Query: 153 SRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKR 212
           +RRL++L+ VR SE+   +K+LY +W +    +   +V++K W+ D+T+N+I+ +VAGKR
Sbjct: 141 NRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK-PVMVDLKSWLEDMTLNMIVRMVAGKR 200

Query: 213 YFGPTAMV---EEAEARACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKE 272
           YFG    V   +  EA  C+KA+ +F  L+G+F + DA P L + D+ GH K MK+T  E
Sbjct: 201 YFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSE 260

Query: 273 LDHILTQWLEEHKKKRA-SGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKA 332
           LD IL +W+E H+++R  SG  + D DF+DVM+S  E+         S   +D +T IK+
Sbjct: 261 LDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQ------GKLSHLQYDANTSIKS 320

Query: 333 TCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQ 392
           TCL ++LGGSDT+   +TWA+SLLLNN   L K Q+E+D H+GRDR +++SD+  LVYLQ
Sbjct: 321 TCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQ 380

Query: 393 AVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKP 452
           A++KET+RLY AG L+  RE  ED  + G+++  GTRL +N WKI RD +V+ EP EF+P
Sbjct: 381 AIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRP 440

Query: 453 ERFLTTH-RDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDISS 501
           ERF+T   ++FDV GQ FEL+PFG GRR+CPG S  +Q++ L L   LH+FD+ +
Sbjct: 441 ERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKT 488

[Close]

BLAST of Moc01g30000 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 448.4 bits (1152), Expect = 8.3e-126
Identity = 222/474 (46.84%), Postives = 326/474 (68.78%), Query Frame = 0


Query: 33  FTAAAASKGLKPPSVPGGWPLIGHLPLLGG-PQLPHVKLGALADEYGPIFSIQLGIHQAV 92
           F  +   K +K P+  G WP+IGHL LL G  QL +  LG +AD+YGP  S++LG  +  
Sbjct: 21  FKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLRLGSSETF 80

Query: 93  VINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLS 152
           V++S+E AK CFT +D A++ RP   AA+ +GY+ AVF F PY ++WR+MRK  TL LLS
Sbjct: 81  VVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLS 140

Query: 153 SRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKR 212
           +RRL++L+ VR SE++  +++LY +W ++   +   +V++K W+ D+++N+++ +VAGKR
Sbjct: 141 NRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE-PVMVDLKSWLEDMSLNMMVRMVAGKR 200

Query: 213 YFGPTAMVEE--AEARACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKEL 272
           YFG  ++  E   EAR C+K +  F  L+G+F + DA P LGW D  GH K MK+T +EL
Sbjct: 201 YFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMKQTGREL 260

Query: 273 DHILTQWLEEHKKKR-ASGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKAT 332
           D IL +W+E H+++R  SG    D DF+DVMLS  E+   + +  D+I      T IK+T
Sbjct: 261 DVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAI------TSIKST 320

Query: 333 CLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQA 392
           CL ++LGGS+T+   +TWA+SLLLNN   L K Q+E+D H+GRDR +++SD+  LVY+QA
Sbjct: 321 CLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQA 380

Query: 393 VVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPE 452
           ++KET+RLY AG L+  RE  ED  + G+++ +GTR+ +N WKI RD RV+ EP EF+PE
Sbjct: 381 IIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPE 440

Query: 453 RFLTTH-RDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDISS 501
           RF+T   ++FDV GQ FEL+PFG GRR+CPG S  +Q++ L L   L +FD+ +
Sbjct: 441 RFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKT 487

[Close]

BLAST of Moc01g30000 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 409.8 bits (1052), Expect = 3.3e-114
Identity = 216/475 (45.47%), Postives = 309/475 (65.05%), Query Frame = 0


Query: 33  FTAAAASKGLKPPSVPGGWPLIGHLPLLGG-PQLPHVKLGALADEYGPIFSIQLGIHQAV 92
           F  +   K +K P+  G WP+IGHL LLGG  QL +  LG +AD YGP  S++LG  +  
Sbjct: 21  FKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLRLGSSETF 80

Query: 93  VINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLS 152
           V +S+E AK CFT +D A++      AA+ +GY            +W +MRK   + LLS
Sbjct: 81  VGSSFEVAKDCFTVNDKALA-SLMTAAAKHMGY-----------VFWLEMRKIAMIELLS 140

Query: 153 SRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKR 212
           +RRL++L +VR SE++  +K+LY +W ++   +   +V++K W+ D+  N+I+ +VAGKR
Sbjct: 141 NRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSE-PVMVDLKSWLEDMIANMIMRMVAGKR 200

Query: 213 YF---GPTAMVEEAEARACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKE 272
           YF   G  +     EAR  +K + +F  L+G+F + DA P LGWLD+ GH K MK+T +E
Sbjct: 201 YFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEMKQTRRE 260

Query: 273 LDHILTQWLEEHKKKR-ASGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKA 332
           LD IL +W+E H+++R  SG    D DF+DVMLS  E+         S   +D +T IK 
Sbjct: 261 LDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQ------GKLSHLQYDANTCIKT 320

Query: 333 TCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQ 392
           TCL ++LGGS+T+   +TWA+SLLLNN   L KVQ+E+D H+GRDR +++SD+  LVYLQ
Sbjct: 321 TCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQ 380

Query: 393 AVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKP 452
           A++KET+RLY A  L+  RE  ED  + G+++  GTRL +N WKI RD +V+ EP EF+P
Sbjct: 381 AIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRP 440

Query: 453 ERFLTTH-RDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDISS 501
           ERF+T   +DFDV GQ FEL+PFG GRR+CPG S  +QM+ L L   LH+F++ +
Sbjct: 441 ERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKT 476

[Close]

BLAST of Moc01g30000 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-105
Identity = 203/459 (44.23%), Postives = 284/459 (61.87%), Query Frame = 0


Query: 45  PSVPGGWPLIGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFT 104
           P VPG WPL+GHL L       HV  GA+AD YGP+F  +LG  + ++INS E AK+ +T
Sbjct: 35  PMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVFMAKLGSIKVMIINSKEVAKEIYT 94

Query: 105 THDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRES 164
            HD  +  RP++ A+++LGYN +   F PY  YWR++RK     L S+  +++    R  
Sbjct: 95  VHDKLLE-RPELTASKLLGYNDSFLTFSPYGLYWREIRKIAVSELFSTSGVDMHMVSRAR 154

Query: 165 EVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEA 224
           E + A + LY  W++R   +   LV+MKQ   DLT N+ L +V+GKRYFG     E  EA
Sbjct: 155 EADLAFRALYVRWEKRGKPKEGVLVDMKQEFIDLTANISLMMVSGKRYFGENPNCEVKEA 214

Query: 225 RACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKR 284
           R C K ++EFL    LF+L D  P LG+LD     + MKRT+K LD +   W+EEHK KR
Sbjct: 215 RRCGKLIREFLDYFALFLLSDVAPVLGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKR 274

Query: 285 ASGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSF-DVDTIIKATCLTMVLGGSDTTTVV 344
            S   + + D++D+++         ++  D I    D  T IKA CL +VL GS+T  VV
Sbjct: 275 -SDHGRSENDYLDILI--------KILGQDKIPGLSDTHTKIKALCLNLVLAGSETAIVV 334

Query: 345 VTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLI 404
           + WA+SLLLNN   L K QEE+D+ IG++R ++E D+ +LVYLQA+VKET RLY    L+
Sbjct: 335 LVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIVKETFRLYPPVPLV 394

Query: 405 -LRETSEDF--AIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNG 464
             R   EDF  A    H+  GT+L ++ WKIHRD  VW  P +F+PERFLT++R+ DV G
Sbjct: 395 AYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPERFLTSNRELDVGG 454

Query: 465 QQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDIS 500
           Q ++  PFG GRR+CP +  G++MV  +L   LH+FD++
Sbjct: 455 QSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLA 482

[Close]

BLAST of Moc01g30000 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 357.8 bits (917), Expect = 1.5e-98
Identity = 195/473 (41.23%), Postives = 284/473 (60.04%), Query Frame = 0


Query: 28  FLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGI 87
           FL K  +         P  + G  PL GHL LL G +L   KL A++ ++GPIFS++LG 
Sbjct: 24  FLRKQLSRCEVDSSTIPEPL-GALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGF 83

Query: 88  HQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQILGYNYAVFAFGPYDSYWRKMRKTTTL 147
           ++ VV +  +  K CFTT+D+A + RP I   + +GYN A     PY  YWR++RK  T+
Sbjct: 84  YRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTV 143

Query: 148 HLLSSRRLELLRDVRESEVNQAIKELYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVV 207
           HL S+  +E+L  +R SEVN  IK LY      +   G  +V++      LT N+IL  +
Sbjct: 144 HLFSNHSIEMLGHIRSSEVNTLIKHLY------KGNGGTSIVKIDMLFEFLTFNIILRKM 203

Query: 208 AGKRY-FGPTAMVEEAEARACQKAMKEFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTS 267
            GKR  FG       ++    ++A+K    L  + ++GD +P+LGWLD   +   MKR  
Sbjct: 204 VGKRIGFGEV----NSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAKN-SQMKRLF 263

Query: 268 KELDHILTQWLEEHKKKRASGKSKEDKDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIK 327
           KELD + T+WL EH KKR+  +  +++  MD++L  L   P+++V S  +     D I+K
Sbjct: 264 KELDSVNTKWLHEHLKKRSRNEKDQERTIMDLLLDIL---PEDIVISGHVR----DVIVK 323

Query: 328 ATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIKVQEEVDAHIGRDRELKESDMSELVYL 387
           AT L + L GSD+T++ +TWA+SLLLNN  AL   QEE+D  +G+ R ++ESD+  L YL
Sbjct: 324 ATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYL 383

Query: 388 QAVVKETMRLYGAGGLI-LRETSEDFAIDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFK 447
           QA+VKET RLY    L  +RE  ED  + G+ +EKGTRL +N WK+HRD ++W +P  FK
Sbjct: 384 QAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFK 443

Query: 448 PERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSFGLQMVQLILGALLHAFDI 499
           PERF+      + +   FE IPFG GRR+CPG++ GL++V  +L  LL  F++
Sbjct: 444 PERFMEDKSQCEKS--NFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFEL 475

The following BLAST results are available for this feature:

BLAST of Moc01g30000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133138.1	0.0e+00	100.00	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]
XP_022132489.1	1.9e-225	84.22	cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P...	[more]
XP_038883369.1	2.2e-184	66.86	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_022133139.1	2.8e-184	88.20	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]
KAG2663852.1	2.9e-165	58.82	hypothetical protein I3760_16G051800 [Carya illinoinensis]	[more]

BLAST of Moc01g30000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49858	6.0e-137	49.80	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49859	9.5e-135	48.68	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q43068	6.8e-133	47.36	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
A0A0N7F297	1.4e-130	50.10	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
Q9SZ46	2.5e-127	48.00	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]

BLAST of Moc01g30000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BY79	0.0e+00	100.00	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=...	[more]
A0A6J1BSL4	9.3e-226	84.22	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=...	[more]
A0A6J1BV48	1.4e-184	88.20	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005814 PE=...	[more]
A0A2I4EQH4	7.0e-165	58.62	cytochrome P450 CYP82D47-like OS=Juglans regia OX=51240 GN=LOC108991732 PE=3 SV=...	[more]
A0A6J5TPS2	3.3e-162	58.50	Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7991 PE=3 SV...	[more]

BLAST of Moc01g30000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	1.8e-128	48.00	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	8.3e-126	46.84	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31950.1	3.3e-114	45.47	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT2G25160.1	1.2e-105	44.23	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	1.5e-98	41.23	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 388..399 score: 41.33 coord: 335..352 score: 34.5 coord: 466..475 score: 58.69
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 45..499 e-value: 4.3E-84 score: 282.9
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 387..405 score: 28.45 coord: 465..475 score: 54.09 coord: 324..341 score: 24.47 coord: 98..119 score: 20.51 coord: 344..370 score: 33.24 coord: 74..93 score: 36.26 coord: 198..216 score: 22.97 coord: 475..498 score: 28.86 coord: 427..451 score: 39.82
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 35..506 e-value: 1.9E-112 score: 378.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 48..500
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 41..498 e-value: 8.8E-19 score: 65.6
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 19..500
None	No IPR available	PANTHER	PTHR47947:SF5	CYTOCHROME P450 FAMILY PROTEIN	coord: 19..500
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 468..477

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g30000.1	Moc01g30000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen