Moc01g29850 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g29850
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionATP-dependent 6-phosphofructokinase
Locationchr1: 21146432 .. 21148550 (+)
RNA-Seq ExpressionMoc01g29850
SyntenyMoc01g29850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGGAACCACCGATCAACAGCGCTCCATCGGCGTCGAAAAGCTCCCCCACCTCACGGACTATTTCCCGGACCTCCAGACCCACACAAACCCTCTCCAGAGCAACCAATTCTTCCACCCATCCGACGGCTTCTACGTCACGCCCACCGACGTCGTCCTCCGCCACACCGCCTTCGACCTCGCCGCCAACTTTCCGGCGACGGCGGCGGCCCCACTCCAGGCGTACCATCGCGCCGGTCCGCGCCGGTCCATCTATTTCGAACCGGGAAGTGTCCGGGCGGCGATCGTCACGTGCGGGGGGCTGTGCCCGGGGATGAACACCGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGGTGTACGGCGTGCGGCGGATCTGTGGGATCGTGGCCGGATACAGAGGGTTTTATTCCGACAACGCGGTGGAGCTGAACCCGAAGCTGGTCCATAACTGGCATAAGAGAGGCGGCACCGCCCTCGAAACCTCCAGAGGGGGATTCGATCTCCACAAAATCGTTGATGCGATACAACATCAGGGTTTCAATCAGGTTTAATTTGTTTCTTTTTCTCTCTGTTTCCACTTTCGATTGGTTCGTTTTTCTTTTTATTTATCCTCTCGATTAGATGGTATAGAAATGAAGGAAAATTTTAGAAATAAAATACTAGAGAAAAGAAAAATCATAGTGTGAAGGTATGGAGTAGTACTCCTTTTTAGCAATAGAATAAAATTTGATAACTATGGAGCAAGATGGAGCAAGGAAAACAAAATAGGATAAATAGTTGTCAAATTTTTTTAATCATGGCGAGTGAAAAAATACATATTCACCAGTAAAGACCTAAGTTTTATCTTTATTATTTTGTTCTTGAAATACATGCGAAAATATATATTTTTTAAAATTTTATTTTAACTTAGATGAAAATAATGTGATTTTATCGCGATAACACATTTTTGGACTTATTTCAAGAAGAAAAACACACACGTTTTTTTTTTTTCCTGAATATCATTGAATCATACGTGAATTTTAAATTTTAATGTTAGCATTATATAATTCTGTTTGAGACTTTGAGTGAGTAAGTAATTACGAATTGTACTAAAAAAAAAGGAAATTACGAATTAAGAAAAGAAGAAAACAAAACAAGAAGTACTATATTTTAAAAACAATTTTCTGGAGAAAAATCTATAATATTTGATCACCATTCACTGCACGTCATGTCCAATAATTTGCAGTTTTTGGTCTTAGGAGTTTGATTGAATTGGGGTTGTTTCAATGGCGATTTGTTGCGAATGAATAGGTCTACATTATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGACGAAATATGTCGTCGGAGACTTCAAGTCGCCGTCACAGGAATTCCGAAAACCGTCGACAACGACGTCGGAATCATCGATCAATCATTTGGATTTCAAACGGCCGTCGAAATGGCGCAGCAGGCAATCAGTGCCGCCCATGTGGAGGCCGAGAGCGCGGTGAACGGCATAGGTCTGGTCAAATTGATGGGCCGAAGCACCGGCCACATCGCTCTTCACGCCACACTGAGCAGCCGAGACGTCGATTGCTGCTTAATACCTGAAATGGCCTTCTACTTGGAAGGCAGCGGAGGGTTGTTTACGTTTCTCGATCAGAAACTGAAGGAGAATGGCCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGGCAAGATTTGATACCTAGAACTGAGGCTCAAAAAGAAGAGAGGGACGAATCTGGAAATCCCGTCTTCTTGGACGTGGGCGGATGGTTGAAGACGGAGCTCAGGAATTGGTGGGCGAGAACGCACCCTGGTGAGTTGTTTACAGTAAAATATATTGATCCTACTTATATGATCCGTGCGGTGCCTGCAAATGCTACGGATAACTCCTACTGTTCCTTGCTGGCTCATTCGGCCATCCATGGCGTAATGGCTGGTTATACTGGATTTGTCCCTGGCCCTATTAATAGACACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACGAGAGATCATAAATGGGCATGGGTTAGATCCGTCACCAATCAGCCTGATTTTGTCAGAAGCTAA

mRNA sequence

ATGGCCGCCGGAACCACCGATCAACAGCGCTCCATCGGCGTCGAAAAGCTCCCCCACCTCACGGACTATTTCCCGGACCTCCAGACCCACACAAACCCTCTCCAGAGCAACCAATTCTTCCACCCATCCGACGGCTTCTACGTCACGCCCACCGACGTCGTCCTCCGCCACACCGCCTTCGACCTCGCCGCCAACTTTCCGGCGACGGCGGCGGCCCCACTCCAGGCGTACCATCGCGCCGGTCCGCGCCGGTCCATCTATTTCGAACCGGGAAGTGTCCGGGCGGCGATCGTCACGTGCGGGGGGCTGTGCCCGGGGATGAACACCGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGGTGTACGGCGTGCGGCGGATCTGTGGGATCGTGGCCGGATACAGAGGGTTTTATTCCGACAACGCGGTGGAGCTGAACCCGAAGCTGGTCCATAACTGGCATAAGAGAGGCGGCACCGCCCTCGAAACCTCCAGAGGGGGATTCGATCTCCACAAAATCGTTGATGCGATACAACATCAGGGTTTCAATCAGGTCTACATTATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGACGAAATATGTCGTCGGAGACTTCAAGTCGCCGTCACAGGAATTCCGAAAACCGTCGACAACGACGTCGGAATCATCGATCAATCATTTGGATTTCAAACGGCCGTCGAAATGGCGCAGCAGGCAATCAGTGCCGCCCATGTGGAGGCCGAGAGCGCGGTGAACGGCATAGGTCTGGTCAAATTGATGGGCCGAAGCACCGGCCACATCGCTCTTCACGCCACACTGAGCAGCCGAGACGTCGATTGCTGCTTAATACCTGAAATGGCCTTCTACTTGGAAGGCAGCGGAGGGTTGTTTACGTTTCTCGATCAGAAACTGAAGGAGAATGGCCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGGCAAGATTTGATACCTAGAACTGAGGCTCAAAAAGAAGAGAGGGACGAATCTGGAAATCCCGTCTTCTTGGACGTGGGCGGATGGTTGAAGACGGAGCTCAGGAATTGGTGGGCGAGAACGCACCCTGGTGAGTTGTTTACAGTAAAATATATTGATCCTACTTATATGATCCGTGCGGTGCCTGCAAATGCTACGGATAACTCCTACTGTTCCTTGCTGGCTCATTCGGCCATCCATGGCGTAATGGCTGGTTATACTGGATTTGTCCCTGGCCCTATTAATAGACACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACGAGAGATCATAAATGGGCATGGGTTAGATCCGTCACCAATCAGCCTGATTTTGTCAGAAGCTAA

Coding sequence (CDS)

ATGGCCGCCGGAACCACCGATCAACAGCGCTCCATCGGCGTCGAAAAGCTCCCCCACCTCACGGACTATTTCCCGGACCTCCAGACCCACACAAACCCTCTCCAGAGCAACCAATTCTTCCACCCATCCGACGGCTTCTACGTCACGCCCACCGACGTCGTCCTCCGCCACACCGCCTTCGACCTCGCCGCCAACTTTCCGGCGACGGCGGCGGCCCCACTCCAGGCGTACCATCGCGCCGGTCCGCGCCGGTCCATCTATTTCGAACCGGGAAGTGTCCGGGCGGCGATCGTCACGTGCGGGGGGCTGTGCCCGGGGATGAACACCGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGGTGTACGGCGTGCGGCGGATCTGTGGGATCGTGGCCGGATACAGAGGGTTTTATTCCGACAACGCGGTGGAGCTGAACCCGAAGCTGGTCCATAACTGGCATAAGAGAGGCGGCACCGCCCTCGAAACCTCCAGAGGGGGATTCGATCTCCACAAAATCGTTGATGCGATACAACATCAGGGTTTCAATCAGGTCTACATTATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGACGAAATATGTCGTCGGAGACTTCAAGTCGCCGTCACAGGAATTCCGAAAACCGTCGACAACGACGTCGGAATCATCGATCAATCATTTGGATTTCAAACGGCCGTCGAAATGGCGCAGCAGGCAATCAGTGCCGCCCATGTGGAGGCCGAGAGCGCGGTGAACGGCATAGGTCTGGTCAAATTGATGGGCCGAAGCACCGGCCACATCGCTCTTCACGCCACACTGAGCAGCCGAGACGTCGATTGCTGCTTAATACCTGAAATGGCCTTCTACTTGGAAGGCAGCGGAGGGTTGTTTACGTTTCTCGATCAGAAACTGAAGGAGAATGGCCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGGCAAGATTTGATACCTAGAACTGAGGCTCAAAAAGAAGAGAGGGACGAATCTGGAAATCCCGTCTTCTTGGACGTGGGCGGATGGTTGAAGACGGAGCTCAGGAATTGGTGGGCGAGAACGCACCCTGGTGAGTTGTTTACAGTAAAATATATTGATCCTACTTATATGATCCGTGCGGTGCCTGCAAATGCTACGGATAACTCCTACTGTTCCTTGCTGGCTCATTCGGCCATCCATGGCGTAATGGCTGGTTATACTGGATTTGTCCCTGGCCCTATTAATAGACACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACGAGAGATCATAAATGGGCATGGGTTAGATCCGTCACCAATCAGCCTGATTTTGTCAGAAGCTAA

Protein sequence

MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Homology
BLAST of Moc01g29850 vs. NCBI nr
Match: XP_022132614.1 (ATP-dependent 6-phosphofructokinase 2 [Momordica charantia])

HSP 1 Score: 948.7 bits (2451), Expect = 1.8e-272
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 60
           MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF
Sbjct: 1   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 60

Query: 61  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 120
           DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE
Sbjct: 61  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 120

Query: 121 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 180
           VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ
Sbjct: 121 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 180

Query: 181 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 240
           GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA
Sbjct: 181 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 240

Query: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 300
           QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL
Sbjct: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 300

Query: 301 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 360
           FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW
Sbjct: 301 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 360

Query: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 420
           ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY
Sbjct: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 420

Query: 421 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Sbjct: 421 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 457

BLAST of Moc01g29850 vs. NCBI nr
Match: XP_038881531.1 (ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-229
Identity = 393/456 (86.18%), Postives = 413/456 (90.57%), Query Frame = 0

Query: 3   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 62
           A  +DQ   I V  LPHLTD FPDL+T TNPLQ+NQFFHP DGFY + TDVVLR  AFDL
Sbjct: 4   AKISDQHPPIAVADLPHLTDDFPDLETRTNPLQTNQFFHPIDGFYASSTDVVLRRIAFDL 63

Query: 63  AANFPATAAAPLQ-AYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 122
                +T  +P   AYHRAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 64  -----STKESPAHVAYHRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 123

Query: 123 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 182
           YGVRRI GI+AGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVD IQH G
Sbjct: 124 YGVRRIYGILAGYRGFYSTEPVELNLKLVDNWHKRGGTALRTSRGGFDLRKIVDTIQHHG 183

Query: 183 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 242
           FNQVYIIGGDGTMRGAVKIFDEI RR LQVAVTGIPKTVDND+GI+D+SFGFQTAVEMAQ
Sbjct: 184 FNQVYIIGGDGTMRGAVKIFDEIRRRNLQVAVTGIPKTVDNDIGIVDRSFGFQTAVEMAQ 243

Query: 243 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 302
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEG GGLF
Sbjct: 244 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGDGGLF 303

Query: 303 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 362
            FL+++LKENGHAVLVVAEGAGQ +IPRTEA+KEERDESGNPVFLDVGGWLKTEL+NWWA
Sbjct: 304 PFLERRLKENGHAVLVVAEGAGQSMIPRTEAEKEERDESGNPVFLDVGGWLKTELKNWWA 363

Query: 363 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 422
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 364 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 423

Query: 423 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Sbjct: 424 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 454

BLAST of Moc01g29850 vs. NCBI nr
Match: XP_008440953.1 (PREDICTED: ATP-dependent 6-phosphofructokinase 2 [Cucumis melo])

HSP 1 Score: 793.9 bits (2049), Expect = 7.4e-226
Identity = 388/455 (85.27%), Postives = 413/455 (90.77%), Query Frame = 0

Query: 3   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 62
           A  +DQ   I V  LPHLTD FP+L+T TNPLQ+NQFFHP DGFY T TD+VLR  AFDL
Sbjct: 4   AKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDL 63

Query: 63  AANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVY 122
           + N   T+ A + AY RAGPRR++YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+Y
Sbjct: 64  SGN---TSPAHV-AYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELY 123

Query: 123 GVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGF 182
           GVRRI GIVAGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVDAI++ GF
Sbjct: 124 GVRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGF 183

Query: 183 NQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQ 242
           NQVYI GGDGTMRGAVKIF+EI RR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQQ
Sbjct: 184 NQVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQ 243

Query: 243 AISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFT 302
           AI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLF 
Sbjct: 244 AINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFP 303

Query: 303 FLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWAR 362
           FL+Q+LKENGHAVLVVAEGAGQ +IPRTEAQKEERDESGNPVFLDVGGWLKTEL+NWW R
Sbjct: 304 FLEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWER 363

Query: 363 THPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSY 422
           THPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YSY
Sbjct: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSY 423

Query: 423 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFV+S
Sbjct: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of Moc01g29850 vs. NCBI nr
Match: XP_022950529.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata])

HSP 1 Score: 793.9 bits (2049), Expect = 7.4e-226
Identity = 386/456 (84.65%), Postives = 413/456 (90.57%), Query Frame = 0

Query: 2   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 61
           AA   D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 5   AAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 64

Query: 62  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 121
           L+   P+ A     AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 65  LSRK-PSPAHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 124

Query: 122 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 181
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 125 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 184

Query: 182 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 241
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 185 FNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 244

Query: 242 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 301
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 245 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 304

Query: 302 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 361
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 305 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 364

Query: 362 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 421
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 365 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 424

Query: 422 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           YIPLK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV +
Sbjct: 425 YIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVET 456

BLAST of Moc01g29850 vs. NCBI nr
Match: KAG6603845.1 (ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034027.1 ATP-dependent 6-phosphofructokinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793.1 bits (2047), Expect = 1.3e-225
Identity = 385/456 (84.43%), Postives = 413/456 (90.57%), Query Frame = 0

Query: 2   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 61
           AA   D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 6   AAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 65

Query: 62  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 121
           L+   P+       AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 66  LSRK-PSPGHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 125

Query: 122 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 181
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 126 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 185

Query: 182 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 241
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 186 FNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 245

Query: 242 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 301
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 246 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 305

Query: 302 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 361
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 306 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 365

Query: 362 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 421
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 366 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 425

Query: 422 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           YIPLK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV++
Sbjct: 426 YIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT 457

BLAST of Moc01g29850 vs. ExPASy Swiss-Prot
Match: Q9FIK0 (ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 3.3e-200
Identity = 336/441 (76.19%), Postives = 379/441 (85.94%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           + KLP L+     L+   NPL+ N +FHPS+GFY+TP+DV+L   A+D +A+  +     
Sbjct: 7   IRKLPSLS----GLRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAHSQSRV--- 66

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
             AYHRAGPRR I +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWE+YGVR I GI AG
Sbjct: 67  --AYHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAG 126

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFYS  AV+L+PK VH+WHK+GGT L TSRGGF L KIVDAI   G+NQVYIIGGDGT
Sbjct: 127 YRGFYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGT 186

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
           MRGAV+IF EI  R+L+V +T IPKTVDNDVGIID+SFGFQTAVEMAQ+AISAAHVEAES
Sbjct: 187 MRGAVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAES 246

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF FL+++LKE GH
Sbjct: 247 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGH 306

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
           AVLVVAEGAGQ++IPR E+QK+ERDESGN VFLDVG W K+ L+ WW R HP ELFTVKY
Sbjct: 307 AVLVVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKY 366

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRAVPANATDN YC+LLAHSAIHGVMAGYTGFVPGPIN +Y+YIPL+EVA+ KN
Sbjct: 367 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKN 426

Query: 434 EVNTRDHKWAWVRSVTNQPDF 455
           +VNTRDHKWAWVRSVTNQPDF
Sbjct: 427 QVNTRDHKWAWVRSVTNQPDF 438

BLAST of Moc01g29850 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.8e-141
Identity = 241/442 (54.52%), Postives = 316/442 (71.49%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           +E +PHLT + PDL ++ NPL+ +Q +      +V+  DVV ++      +         
Sbjct: 71  LEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGV----- 130

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR  +YF    V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  G
Sbjct: 131 --HFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGG 190

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFYS N + L PK+V++ HKRGGT L+TSRGG D  KIVD IQ +G NQVYIIGG GT
Sbjct: 191 YRGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGT 250

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            +GA KI++E+ RR LQVAV+GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVE ES
Sbjct: 251 QKGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVES 310

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NG+G+VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++++LKEN H
Sbjct: 311 VENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRH 370

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQD + ++    E +D SGN + LDVG WL  ++++ +       +  +KY
Sbjct: 371 MVIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKY 430

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRA+P+NA+DN YC+LLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N
Sbjct: 431 IDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTN 490

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
            V   D  WA + + TNQP F+
Sbjct: 491 TVKLTDRMWARLLASTNQPSFL 504

BLAST of Moc01g29850 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 4.2e-139
Identity = 247/442 (55.88%), Postives = 317/442 (71.72%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           +E +PHL+DY  DL T+ NPLQSN  +     ++V   D V       +  + P      
Sbjct: 21  LEDVPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDTVQEKIV--VHKDSPRGT--- 80

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR+ +YF+P  VRA IVTCGGLCPG+NTVIRE+V GL  +YGV  + G+  G
Sbjct: 81  --HFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCG 140

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           +RGFYS N V L PK V + HKRGGT L TSRGG D  KIVD IQ +  NQVYIIGGDGT
Sbjct: 141 FRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGT 200

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            +GA  I+ EI RR L+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEA S
Sbjct: 201 QKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATS 260

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NGIG+VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ ++L+ENGH
Sbjct: 261 VENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGH 320

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQDL+  +    E++D SGN +  DVG W+  +++ ++A+ +  ++ T+KY
Sbjct: 321 MVIVIAEGAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKY 380

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRA+PANA+DN Y +LLA SA+HG MAGYTGFV G +N  ++YIP   + E +N
Sbjct: 381 IDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQN 440

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
           +V   D  WA + S TNQP F+
Sbjct: 441 KVVITDRMWARMLSSTNQPSFM 451

BLAST of Moc01g29850 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 5.5e-139
Identity = 247/442 (55.88%), Postives = 316/442 (71.49%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           ++ +PHL DY PDL T+ NPLQ N  +     ++V   D V       +  + P      
Sbjct: 20  LQDVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVV--VHKDGPRGV--- 79

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR+ +YFE   V A IVTCGGLCPG+NTVIRE+V  L  +YGV+RI GI  G
Sbjct: 80  --HFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGG 139

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFY+ N + LN K+V++ HKRGGT + TSRGG D +KIVD+IQ +G NQVYIIGGDGT
Sbjct: 140 YRGFYAKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGT 199

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            RGA  IF+EI RRRL+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEAES
Sbjct: 200 QRGASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAES 259

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NGIG VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG GGLF F++++LK++GH
Sbjct: 260 NENGIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGH 319

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQDL+ ++  +    D SGN +  DVG WL   +++ + +     +  +KY
Sbjct: 320 MVIVLAEGAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKY 379

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRAVP+NA+DN YC+LLA SA+HG MAGYTG+  G +N   +YIP   + E +N
Sbjct: 380 IDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQN 439

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
            V   D  WA + S TNQP F+
Sbjct: 440 NVVITDRMWARLLSSTNQPSFL 451

BLAST of Moc01g29850 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 7.1e-139
Identity = 248/442 (56.11%), Postives = 315/442 (71.27%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           +E +PHL+DY P L T+ NPLQ N  +     ++V   D V +     +  + P      
Sbjct: 20  LEDVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIV--VHKDGPRGI--- 79

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR+ +YFE   V A IVTCGGLCPG+NTVIRE+V  L  +YGV+RI GI  G
Sbjct: 80  --HFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGG 139

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFY+ N V L+ K+V++ HKRGGT L TSRGG D  KIVD+IQ +G NQVYIIGGDGT
Sbjct: 140 YRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGT 199

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            RGA  IF+EI RR L+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEAES
Sbjct: 200 QRGASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAES 259

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NGIG+VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGLF +++++LKE+GH
Sbjct: 260 IENGIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGH 319

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            VLV+AEGAGQDL+ ++      +D SGN +  DVG WL   +++ +       +  +KY
Sbjct: 320 MVLVIAEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKY 379

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRAVP+NA+DN YC+LLA SA+HG MAGYTG++ G +N   +YIP   + E +N
Sbjct: 380 IDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQN 439

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
            V   D  WA + S TNQP F+
Sbjct: 440 HVVITDRMWARLLSSTNQPSFL 452

BLAST of Moc01g29850 vs. ExPASy TrEMBL
Match: A0A6J1BTK2 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111005433 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 8.7e-273
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 60
           MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF
Sbjct: 1   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 60

Query: 61  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 120
           DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE
Sbjct: 61  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 120

Query: 121 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 180
           VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ
Sbjct: 121 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 180

Query: 181 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 240
           GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA
Sbjct: 181 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 240

Query: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 300
           QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL
Sbjct: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 300

Query: 301 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 360
           FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW
Sbjct: 301 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 360

Query: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 420
           ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY
Sbjct: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 420

Query: 421 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Sbjct: 421 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 457

BLAST of Moc01g29850 vs. ExPASy TrEMBL
Match: A0A6J1GG28 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111453612 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 386/456 (84.65%), Postives = 413/456 (90.57%), Query Frame = 0

Query: 2   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 61
           AA   D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 5   AAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 64

Query: 62  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 121
           L+   P+ A     AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 65  LSRK-PSPAHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 124

Query: 122 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 181
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 125 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 184

Query: 182 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 241
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 185 FNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 244

Query: 242 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 301
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 245 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 304

Query: 302 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 361
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 305 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 364

Query: 362 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 421
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 365 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 424

Query: 422 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           YIPLK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV +
Sbjct: 425 YIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVET 456

BLAST of Moc01g29850 vs. ExPASy TrEMBL
Match: A0A1S3B325 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 388/455 (85.27%), Postives = 413/455 (90.77%), Query Frame = 0

Query: 3   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 62
           A  +DQ   I V  LPHLTD FP+L+T TNPLQ+NQFFHP DGFY T TD+VLR  AFDL
Sbjct: 4   AKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDL 63

Query: 63  AANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVY 122
           + N   T+ A + AY RAGPRR++YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+Y
Sbjct: 64  SGN---TSPAHV-AYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELY 123

Query: 123 GVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGF 182
           GVRRI GIVAGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVDAI++ GF
Sbjct: 124 GVRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGF 183

Query: 183 NQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQ 242
           NQVYI GGDGTMRGAVKIF+EI RR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQQ
Sbjct: 184 NQVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQ 243

Query: 243 AISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFT 302
           AI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLF 
Sbjct: 244 AINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFP 303

Query: 303 FLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWAR 362
           FL+Q+LKENGHAVLVVAEGAGQ +IPRTEAQKEERDESGNPVFLDVGGWLKTEL+NWW R
Sbjct: 304 FLEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWER 363

Query: 363 THPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSY 422
           THPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YSY
Sbjct: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSY 423

Query: 423 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFV+S
Sbjct: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of Moc01g29850 vs. ExPASy TrEMBL
Match: A0A6J1ISR0 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.1e-226
Identity = 385/456 (84.43%), Postives = 414/456 (90.79%), Query Frame = 0

Query: 2   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 61
           AA  +D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 7   AAEISDRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 66

Query: 62  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 121
           L+   P+ A     AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 67  LSRK-PSPAHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 126

Query: 122 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 181
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 127 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 186

Query: 182 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 241
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 187 FNQVYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 246

Query: 242 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 301
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 247 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 306

Query: 302 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 361
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 307 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 366

Query: 362 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 421
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 367 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 426

Query: 422 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           YIPLK+VAEAKNEVNT+DHKWAWVRSVTNQPDFV +
Sbjct: 427 YIPLKDVAEAKNEVNTKDHKWAWVRSVTNQPDFVET 458

BLAST of Moc01g29850 vs. ExPASy TrEMBL
Match: A0A5D3CPV9 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.4e-225
Identity = 387/455 (85.05%), Postives = 412/455 (90.55%), Query Frame = 0

Query: 3   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 62
           A  +DQ   I V  LPHLTD FP+L+T TNPLQ+NQFFHP DGFY T TD+VLR  AFDL
Sbjct: 4   AKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDL 63

Query: 63  AANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVY 122
           + N   T+ A + AY RAGPRR++YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+Y
Sbjct: 64  SGN---TSPAHV-AYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELY 123

Query: 123 GVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGF 182
           GVRRI GIVAGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVDAI++ GF
Sbjct: 124 GVRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGF 183

Query: 183 NQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQ 242
           NQVYI GGDGTMRGAVKIF+EI RR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQQ
Sbjct: 184 NQVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQ 243

Query: 243 AISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFT 302
           AI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLF 
Sbjct: 244 AINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFP 303

Query: 303 FLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWAR 362
           FL+Q+LKENGHAVLVVAEGAGQ +IPRTEAQKEERDESGNPVFLDVGGWLKTEL+NWW R
Sbjct: 304 FLEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWER 363

Query: 363 THPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSY 422
           THPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YSY
Sbjct: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSY 423

Query: 423 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 458
           IPLKEVAEAKNEVNTRDHKWAWVRSV NQPDFV+S
Sbjct: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVANQPDFVKS 454

BLAST of Moc01g29850 vs. TAIR 10
Match: AT5G47810.1 (phosphofructokinase 2 )

HSP 1 Score: 699.1 bits (1803), Expect = 2.3e-201
Identity = 336/441 (76.19%), Postives = 379/441 (85.94%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           + KLP L+     L+   NPL+ N +FHPS+GFY+TP+DV+L   A+D +A+  +     
Sbjct: 7   IRKLPSLS----GLRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAHSQSRV--- 66

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
             AYHRAGPRR I +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWE+YGVR I GI AG
Sbjct: 67  --AYHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAG 126

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFYS  AV+L+PK VH+WHK+GGT L TSRGGF L KIVDAI   G+NQVYIIGGDGT
Sbjct: 127 YRGFYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGT 186

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
           MRGAV+IF EI  R+L+V +T IPKTVDNDVGIID+SFGFQTAVEMAQ+AISAAHVEAES
Sbjct: 187 MRGAVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAES 246

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF FL+++LKE GH
Sbjct: 247 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGH 306

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
           AVLVVAEGAGQ++IPR E+QK+ERDESGN VFLDVG W K+ L+ WW R HP ELFTVKY
Sbjct: 307 AVLVVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKY 366

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRAVPANATDN YC+LLAHSAIHGVMAGYTGFVPGPIN +Y+YIPL+EVA+ KN
Sbjct: 367 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKN 426

Query: 434 EVNTRDHKWAWVRSVTNQPDF 455
           +VNTRDHKWAWVRSVTNQPDF
Sbjct: 427 QVNTRDHKWAWVRSVTNQPDF 438

BLAST of Moc01g29850 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 502.3 bits (1292), Expect = 4.1e-142
Identity = 241/442 (54.52%), Postives = 316/442 (71.49%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           +E +PHLT + PDL ++ NPL+ +Q +      +V+  DVV ++      +         
Sbjct: 71  LEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGV----- 130

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR  +YF    V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  G
Sbjct: 131 --HFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGG 190

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFYS N + L PK+V++ HKRGGT L+TSRGG D  KIVD IQ +G NQVYIIGG GT
Sbjct: 191 YRGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGT 250

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            +GA KI++E+ RR LQVAV+GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVE ES
Sbjct: 251 QKGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVES 310

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NG+G+VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++++LKEN H
Sbjct: 311 VENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRH 370

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQD + ++    E +D SGN + LDVG WL  ++++ +       +  +KY
Sbjct: 371 MVIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKY 430

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRA+P+NA+DN YC+LLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N
Sbjct: 431 IDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTN 490

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
            V   D  WA + + TNQP F+
Sbjct: 491 TVKLTDRMWARLLASTNQPSFL 504

BLAST of Moc01g29850 vs. TAIR 10
Match: AT5G61580.2 (phosphofructokinase 4 )

HSP 1 Score: 496.9 bits (1278), Expect = 1.7e-140
Identity = 241/442 (54.52%), Postives = 315/442 (71.27%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           +E +PHLT + PDL ++ NPL+ +Q +      +V+  DVV ++      +         
Sbjct: 71  LEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGV----- 130

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR  +YF    V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  G
Sbjct: 131 --HFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGG 190

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFYS N + L PK+V++ HKRGGT L+TSRGG D  KIVD IQ +G NQVYIIGG GT
Sbjct: 191 YRGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGT 250

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            +GA KI++E+ RR LQVAV+GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVE ES
Sbjct: 251 QKGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVES 310

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NG+G+VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++++LKEN H
Sbjct: 311 VENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRH 370

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQD + ++    E +D SGN + LDVG WL  ++++ +       +  +KY
Sbjct: 371 MVIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKY 430

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRA+P+NA+DN YC+LLA SA+HG MAGY+GF  GP+N  ++YIP+  V E  N
Sbjct: 431 IDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPI-SVTEVTN 490

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
            V   D  WA + + TNQP F+
Sbjct: 491 TVKLTDRMWARLLASTNQPSFL 503

BLAST of Moc01g29850 vs. TAIR 10
Match: AT4G32840.1 (phosphofructokinase 6 )

HSP 1 Score: 496.1 bits (1276), Expect = 3.0e-140
Identity = 247/442 (55.88%), Postives = 317/442 (71.72%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           +E +PHL+DY  DL T+ NPLQSN  +     ++V   D V       +  + P      
Sbjct: 21  LEDVPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDTVQEKIV--VHKDSPRGT--- 80

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR+ +YF+P  VRA IVTCGGLCPG+NTVIRE+V GL  +YGV  + G+  G
Sbjct: 81  --HFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCG 140

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           +RGFYS N V L PK V + HKRGGT L TSRGG D  KIVD IQ +  NQVYIIGGDGT
Sbjct: 141 FRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGT 200

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            +GA  I+ EI RR L+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEA S
Sbjct: 201 QKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATS 260

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NGIG+VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ ++L+ENGH
Sbjct: 261 VENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGH 320

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQDL+  +    E++D SGN +  DVG W+  +++ ++A+ +  ++ T+KY
Sbjct: 321 MVIVIAEGAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKY 380

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRA+PANA+DN Y +LLA SA+HG MAGYTGFV G +N  ++YIP   + E +N
Sbjct: 381 IDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQN 440

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
           +V   D  WA + S TNQP F+
Sbjct: 441 KVVITDRMWARMLSSTNQPSFM 451

BLAST of Moc01g29850 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 495.7 bits (1275), Expect = 3.9e-140
Identity = 247/442 (55.88%), Postives = 316/442 (71.49%), Query Frame = 0

Query: 14  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 73
           ++ +PHL DY PDL T+ NPLQ N  +     ++V   D V       +  + P      
Sbjct: 20  LQDVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVV--VHKDGPRGV--- 79

Query: 74  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 133
              + RAGPR+ +YFE   V A IVTCGGLCPG+NTVIRE+V  L  +YGV+RI GI  G
Sbjct: 80  --HFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGG 139

Query: 134 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 193
           YRGFY+ N + LN K+V++ HKRGGT + TSRGG D +KIVD+IQ +G NQVYIIGGDGT
Sbjct: 140 YRGFYAKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGT 199

Query: 194 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 253
            RGA  IF+EI RRRL+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEAES
Sbjct: 200 QRGASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAES 259

Query: 254 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 313
             NGIG VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG GGLF F++++LK++GH
Sbjct: 260 NENGIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGH 319

Query: 314 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 373
            V+V+AEGAGQDL+ ++  +    D SGN +  DVG WL   +++ + +     +  +KY
Sbjct: 320 MVIVLAEGAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKY 379

Query: 374 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 433
           IDPTYMIRAVP+NA+DN YC+LLA SA+HG MAGYTG+  G +N   +YIP   + E +N
Sbjct: 380 IDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQN 439

Query: 434 EVNTRDHKWAWVRSVTNQPDFV 456
            V   D  WA + S TNQP F+
Sbjct: 440 NVVITDRMWARLLSSTNQPSFL 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132614.11.8e-272100.00ATP-dependent 6-phosphofructokinase 2 [Momordica charantia][more]
XP_038881531.11.9e-22986.18ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida][more]
XP_008440953.17.4e-22685.27PREDICTED: ATP-dependent 6-phosphofructokinase 2 [Cucumis melo][more]
XP_022950529.17.4e-22684.65ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata][more]
KAG6603845.11.3e-22584.43ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
Q9FIK03.3e-20076.19ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE... [more]
Q9FKG35.8e-14154.52ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9M0764.2e-13955.88ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE... [more]
Q9C5J75.5e-13955.88ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Q94AA47.1e-13956.11ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1BTK28.7e-273100.00ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110054... [more]
A0A6J1GG283.6e-22684.65ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11145361... [more]
A0A1S3B3253.6e-22685.27ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3... [more]
A0A6J1ISR06.1e-22684.43ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 ... [more]
A0A5D3CPV91.4e-22585.05ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF... [more]
Match NameE-valueIdentityDescription
AT5G47810.12.3e-20176.19phosphofructokinase 2 [more]
AT5G61580.14.1e-14254.52phosphofructokinase 4 [more]
AT5G61580.21.7e-14054.52phosphofructokinase 4 [more]
AT4G32840.13.0e-14055.88phosphofructokinase 6 [more]
AT5G56630.13.9e-14055.88phosphofructokinase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 232..250
score: 26.05
coord: 253..269
score: 28.51
coord: 97..116
score: 45.13
coord: 181..197
score: 43.89
coord: 214..231
score: 35.61
coord: 310..322
score: 37.28
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 95..402
e-value: 2.3E-54
score: 184.6
NoneNo IPR availableGENE3D3.40.50.450coord: 89..278
e-value: 4.0E-55
score: 188.0
NoneNo IPR availablePANTHERPTHR45770:SF9ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 2coord: 9..457
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 9..457
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typePIRSFPIRSF000534ATP_PFK_TP0108coord: 3..457
e-value: 3.6E-170
score: 564.2
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typeHAMAPMF_01981Phosphofructokinase_II_Xcoord: 17..455
score: 51.375706
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 21..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g29850.1Moc01g29850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding