Moc01g29410 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g29410
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr1: 20861891 .. 20864062 (-)
RNA-Seq Expression	Moc01g29410
Synteny	Moc01g29410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAGCACCGGCAATCTCGTTTTCCTGCTCGGAAGTCGTCGTCTTCTTCTACTCTCATATTTACCTTGCTAATTATGTTTACCTTCGTCATTCTCATTCTTCTTGCCCTCGGAATCCTCTCGATCCCCGGCAATTCCGGTGATTCGCCCAAGGCTCATGATCTGAGCTCCATTGTGCGCAAAACTTCCGACGAGTATGTGATCTTGTGGCTTTGCTTCCATTTTTTCGTTCTGATTAGGATTAGCTGTAATTAATTGTGTGGATTGCTTGAGTTTACCTAGTATTGAGGAGGAGAAAGGGGAGCAGTGGGTTGAAGTGATCTCCTGGGAACCTAGAGCCTTTGTTTATCACAACTTTCTGGTAAGTTTTTTGTATTTCTTCCGATCTATAACGAGAGATTTGTCTTTATGTCCAGTTTTCTTGGTGATGACTTACGTTGTCTCTTTCTTTGTGAAATTATTGTATGGAATTCTTAAACTTGGTTGCTGTATGCTCATACGTTATACATGGTAAATGTGTCTTTGGAACTGGGGCTGCATATGCGAAAGAAAGCTAAGGGCATGTTTGAGATGAAGTCTTTGAACTTGTTATGGAACTCTGTCTTATTGTGTTGAAGGGGTTAAGGATAATTCTCATCTGTATTTGTCTTTGGATTGTAGTGGGGTTTGAGTTGACTGAGGCCTGTTTATTAATTCCGAAGTAGTGAATTACTGAATTGGTTAACTTTTTAATTTCTCAGACTAAGGAGGAATGTGAGTACCTAGTCAGCCTTGCAAAGCCTAACATGCAGAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGGTGTTCTATTCTCTTCCCCAGACTTCTCTATTGTTAGTAATGCTGGATCTCTAATGTGGAAACCTCTGTGACCTTTCTTTCAGAGTTCGCACCAGCTCCGGAACATTTTTGCCTAGAGGACGTGATAAGACTATAAGAAACATTGAGAAAAGGATTGCTGATTTCTCCTTCATTCCCATGGGTACATTTTTTGAGCCTCTTTCCCTCTTGATGATTTCCTCCCGCTAGGACAATAATTAATTCTCATCTTGCAAGGTTAAAATAAAATTTTGGTAATTTTGTAATTTATTTCTGAGTGCACGTATCATTGCAGAATTTTTTTTCCCTATAGTCTAACATTGTCAATTATAAATTTCCTTTTGTTAAGAAAAATCTTTACAGTTATGCCCAAGACCATTCGAATAGACACAATTACTGTGCTAAGTTTCACATGGACGGGGAAGATTTTTAATCATTGTTGAAAAGTTGAAAAACGAACACCCCCTCAAATGTAGGAACACAAAAATGGTCCCGATATGGTCTTATGAGCTTACCCACCCATTGTCCCTTTTAAGAATGAAAGAGAACAGCGTTCACCTGATCATATGTGCCAATATTTCTTTATCATAAGTAGCTATCGTCTTGTTATGTTTTGGATTTTAATGTTATACATCCCTTTCATATCTTCTTACTGAAGCTCACACATTAAATATGCAGAGCATGGAGAAGGACTTCAGGTTCTTCATTATGAAGTGGGACAGAAATACGAACCTCATTTTGATTATTTCCTTGATGACTTCAATACAAAAAATGGAGGTCAACGTATAGCAACGGTGCTTATGTATCTGTAAGCAGTATTCCTTATTGTTCTTCATTATGCTTTGGTTTACAAACAAGTGCTGTTTTATCATGCATTGGTCCTCCATTATTTTATATGACTTCAATCATAATTCAGCTCAGAAGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCCGCCAAAGGGAATATCAGTTCTGTACCTTGGTGGAACGAGCTTTCAGAATGTGGGAAGAAAGGACTTTCTGTTAAACCGAAAATGGGTGATGCGTTACTTTTCTGGAGCATGAAGCCTGATGCTTCTCTAGATCCATCAAGTTTGCATGGTTAGATACTCTTAACTTATAATTTACCATTCATTTTACAGAGCATGATAGGTTTCAGTGATGCTTGTTTATATGATGGGAAGTATTGGATTTTCTTGATAAGATGACGCCATTCCTTCTCCTGTTCATTTATAGGTGGTTGTCCTGTTATCAAGGGGAATAAGTGGTCTGCTACTAAATGGATGCGAGTAAACGAATACAAAGCTTGA

mRNA sequence

ATGGCGAAGCACCGGCAATCTCGTTTTCCTGCTCGGAAGTCGTCGTCTTCTTCTACTCTCATATTTACCTTGCTAATTATGTTTACCTTCGTCATTCTCATTCTTCTTGCCCTCGGAATCCTCTCGATCCCCGGCAATTCCGGTGATTCGCCCAAGGCTCATGATCTGAGCTCCATTGTGCGCAAAACTTCCGACGATATTGAGGAGGAGAAAGGGGAGCAGTGGGTTGAAGTGATCTCCTGGGAACCTAGAGCCTTTGTTTATCACAACTTTCTGACTAAGGAGGAATGTGAGTACCTAGTCAGCCTTGCAAAGCCTAACATGCAGAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACCAGCTCCGGAACATTTTTGCCTAGAGGACGTGATAAGACTATAAGAAACATTGAGAAAAGGATTGCTGATTTCTCCTTCATTCCCATGGAGCATGGAGAAGGACTTCAGGTTCTTCATTATGAAGTGGGACAGAAATACGAACCTCATTTTGATTATTTCCTTGATGACTTCAATACAAAAAATGGAGGTCAACGTATAGCAACGGTGCTTATGTATCTCTCAGAAGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCCGCCAAAGGGAATATCAGTTCTGTACCTTGGTGGAACGAGCTTTCAGAATGTGGGAAGAAAGGACTTTCTGTTAAACCGAAAATGGGTGATGCGTTACTTTTCTGGAGCATGAAGCCTGATGCTTCTCTAGATCCATCAAGTTTGCATGGTGGTTGTCCTGTTATCAAGGGGAATAAGTGGTCTGCTACTAAATGGATGCGAGTAAACGAATACAAAGCTTGA

Coding sequence (CDS)

ATGGCGAAGCACCGGCAATCTCGTTTTCCTGCTCGGAAGTCGTCGTCTTCTTCTACTCTCATATTTACCTTGCTAATTATGTTTACCTTCGTCATTCTCATTCTTCTTGCCCTCGGAATCCTCTCGATCCCCGGCAATTCCGGTGATTCGCCCAAGGCTCATGATCTGAGCTCCATTGTGCGCAAAACTTCCGACGATATTGAGGAGGAGAAAGGGGAGCAGTGGGTTGAAGTGATCTCCTGGGAACCTAGAGCCTTTGTTTATCACAACTTTCTGACTAAGGAGGAATGTGAGTACCTAGTCAGCCTTGCAAAGCCTAACATGCAGAAGTCTACCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGCACCAGCTCCGGAACATTTTTGCCTAGAGGACGTGATAAGACTATAAGAAACATTGAGAAAAGGATTGCTGATTTCTCCTTCATTCCCATGGAGCATGGAGAAGGACTTCAGGTTCTTCATTATGAAGTGGGACAGAAATACGAACCTCATTTTGATTATTTCCTTGATGACTTCAATACAAAAAATGGAGGTCAACGTATAGCAACGGTGCTTATGTATCTCTCAGAAGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCCGCCAAAGGGAATATCAGTTCTGTACCTTGGTGGAACGAGCTTTCAGAATGTGGGAAGAAAGGACTTTCTGTTAAACCGAAAATGGGTGATGCGTTACTTTTCTGGAGCATGAAGCCTGATGCTTCTCTAGATCCATCAAGTTTGCATGGTGGTTGTCCTGTTATCAAGGGGAATAAGTGGTCTGCTACTAAATGGATGCGAGTAAACGAATACAAAGCTTGA

Protein sequence

MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIVRKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA

Homology

[Close]

BLAST of Moc01g29410 vs. NCBI nr
Match: XP_022133302.1 (probable prolyl 4-hydroxylase 10 [Momordica charantia])

HSP 1 Score: 582.4 bits (1500), Expect = 2.1e-162
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV
Sbjct: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK
Sbjct: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP
Sbjct: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA
Sbjct: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 287

[Close]

BLAST of Moc01g29410 vs. NCBI nr
Match: XP_038881910.1 (probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 554.3 bits (1427), Expect = 6.2e-154
Identity = 269/286 (94.06%), Postives = 278/286 (97.20%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG SPK HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDD++EE+GEQWVEVISWEPRAFVYHNFLTKEECEYL+SLA P+MQKSTVVDSETGK
Sbjct: 61  RKTSDDVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIR IEKRIADFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGN SSVPWWNELSECGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYK
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286

[Close]

BLAST of Moc01g29410 vs. NCBI nr
Match: XP_008440878.1 (PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo])

HSP 1 Score: 551.2 bits (1419), Expect = 5.2e-153
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG S K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDD++EEKGEQWVEVISWEPRAF+YHNFLTKEECEYL+SLAKP+MQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIR IEKRI+DFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Moc01g29410 vs. NCBI nr
Match: XP_004134841.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prolyl 4-hydroxylase 10 [Cucumis sativus] >KGN48959.1 hypothetical protein Csa_003630 [Cucumis sativus])

HSP 1 Score: 550.4 bits (1417), Expect = 8.9e-153
Identity = 265/287 (92.33%), Postives = 279/287 (97.21%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG S K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDD++EEKGEQWVEVISWEPRAFVYHNFLTKEECEYL+SLAKP+MQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+R IEKR++DFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Moc01g29410 vs. NCBI nr
Match: XP_038881909.1 (probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida])

HSP 1 Score: 547.0 bits (1408), Expect = 9.9e-152
Identity = 268/290 (92.41%), Postives = 278/290 (95.86%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG SPK HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDD----IEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDS 120
           RKTSD+    ++EE+GEQWVEVISWEPRAFVYHNFLTKEECEYL+SLA P+MQKSTVVDS
Sbjct: 61  RKTSDEYVISVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDS 120

Query: 121 ETGKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEP 180
           ETGKSKDSRVRTSSGTFLPRGRDKTIR IEKRIADFSFIP+EHGEGLQVLHYEVGQKYEP
Sbjct: 121 ETGKSKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEP 180

Query: 181 HFDYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGL 240
           HFDYFLD++NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGN SSVPWWNELSECGKKGL
Sbjct: 181 HFDYFLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGL 240

Query: 241 SVKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           SVKPK GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYK
Sbjct: 241 SVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 290

[Close]

BLAST of Moc01g29410 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 5.5e-129
Identity = 223/288 (77.43%), Postives = 256/288 (88.89%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MA+ R  R P+ + SS STL+F +LIM TFVILILLA GILS+P N+  S KA+DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSET 120
           RKT      ++ K E+WVE+ISWEPRA VYHNFLTKEEC+YL+ LAKP+M+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDKTIR IEKRI+DF+FIP+EHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+D++NT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN S+VPWWNELSECGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           KPKMGDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV+EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of Moc01g29410 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 1.8e-119
Identity = 211/288 (73.26%), Postives = 247/288 (85.76%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAK R SRF ARK S+   ++F +L M T V+L+LLA G+ S+P N+ +S    DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSDDIE--EEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSET 120
           R  ++  E   ++G+QW EV+SWEPRAFVYHNFL+KEECEYL+SLAKP+M KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHF 180
           GKSKDSRVRTSSGTFL RGRDK I+ IEKRIAD++FIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+D+FNTKNGGQR+AT+LMYLS+VEEGGETVFPAA  N SSVPW+NELSECGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           KP+MGDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of Moc01g29410 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 1.8e-119
Identity = 208/281 (74.02%), Postives = 244/281 (86.83%), Query Frame = 0


Query: 8   RFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  + +S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGKSKDSR 127
             EE  GE+WVEVISWEPRA VYHNFLT EECE+L+SLAKP+M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDYFLDDF 187
           VRTSSGTFL RG D+ +  IEKRI+DF+FIP+E+GEGLQVLHY+VGQKYEPH+DYFLD+F
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKPKMGDA 247
           NTKNGGQRIATVLMYLS+V++GGETVFPAA+GNIS+VPWWNELS+CGK+GLSV PK  DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V+E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of Moc01g29410 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 8.0e-112
Identity = 198/288 (68.75%), Postives = 235/288 (81.60%), Query Frame = 0


Query: 3   KHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSE 122
              R++  D E+  G++W+EVISWEPRAFVYHNFLT EECE+L+SLAKP+M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+ +  IE RI+DF+FIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLS 242
            DYF D+FN + GGQRIATVLMYLS+V+EGGETVFPAAKGN+S VPWW+ELS+CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEY 286
           V PK  DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V+EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of Moc01g29410 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.6e-64
Identity = 117/212 (55.19%), Postives = 161/212 (75.94%), Query Frame = 0


Query: 76  VEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGKSKDSRVRTSSGTFLP 135
           V+ +S +PRAFVY  FLT+ EC+++VSLAK ++++S V D+++G+SK S VRTSSGTF+ 
Sbjct: 37  VKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFIS 96

Query: 136 RGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDYFLDDFNTKNGGQRIA 195
           +G+D  +  IE +I+ ++F+P E+GE +QVL YE GQKY+ HFDYF D  N   GG R+A
Sbjct: 97  KGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMA 156

Query: 196 TVLMYLSEVEEGGETVFPAAKGNISSVPWWN--ELSECGKKGLSVKPKMGDALLFWSMKP 255
           T+LMYLS V +GGETVFP A+     V   N  +LS+C K+G++VKP+ GDALLF+++ P
Sbjct: 157 TILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHP 216

Query: 256 DASLDPSSLHGGCPVIKGNKWSATKWMRVNEY 286
           DA  DP SLHGGCPVI+G KWSATKW+ V+ +
Sbjct: 217 DAIPDPLSLHGGCPVIEGEKWSATKWIHVDSF 248

[Close]

BLAST of Moc01g29410 vs. ExPASy TrEMBL
Match: A0A6J1BWA6 (probable prolyl 4-hydroxylase 10 OS=Momordica charantia OX=3673 GN=LOC111005915 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.0e-162
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV
Sbjct: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK
Sbjct: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP
Sbjct: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA
Sbjct: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 287

[Close]

BLAST of Moc01g29410 vs. ExPASy TrEMBL
Match: A0A1S3B1P6 (probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.5e-153
Identity = 266/287 (92.68%), Postives = 279/287 (97.21%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG S K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDD++EEKGEQWVEVISWEPRAF+YHNFLTKEECEYL+SLAKP+MQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKTIR IEKRI+DFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Moc01g29410 vs. ExPASy TrEMBL
Match: A0A0A0KJH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507320 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.3e-153
Identity = 265/287 (92.33%), Postives = 279/287 (97.21%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNSG S K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSDD++EEKGEQWVEVISWEPRAFVYHNFLTKEECEYL+SLAKP+MQKSTVVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDKT+R IEKR++DFSFIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Moc01g29410 vs. ExPASy TrEMBL
Match: A0A6J1EFG6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.1e-151
Identity = 264/287 (91.99%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSG +PKAHDLSSIV
Sbjct: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSG-APKAHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTSD+++EEKGEQW EVISWEPRAFVYHNFLTKEECEYL+SLAKP+MQKS+VVDSETGK
Sbjct: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK IRNIEKRIADFSF+P+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLD++NTKNGGQRIAT+LMYLS+VEEGGETVFPAAKGN SSVPWW+ELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of Moc01g29410 vs. ExPASy TrEMBL
Match: A0A6J1IRF1 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=4 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 6.9e-151
Identity = 263/287 (91.64%), Postives = 276/287 (96.17%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAKHRQSRFP RKSSSSST+IFTLLIMFTFVILILLALGILSIPGNSG SPK HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGK 120
           RKTS+D++EEKGE+W EVISWEPRAFVYHNFLTKEECEYL+S AKP+MQKSTVVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DK I  IEKRIADF+FIP+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKP 240
           FLDD+NTKNGGQRIATVLMYLS+VEEGGETVFPAAKGN SSVPWWNELS+CGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYKA 288
           K GDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of Moc01g29410 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 461.8 bits (1187), Expect = 3.9e-130
Identity = 223/288 (77.43%), Postives = 256/288 (88.89%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MA+ R  R P+ + SS STL+F +LIM TFVILILLA GILS+P N+  S KA+DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSET 120
           RKT      ++ K E+WVE+ISWEPRA VYHNFLTKEEC+YL+ LAKP+M+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDKTIR IEKRI+DF+FIP+EHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+D++NT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN S+VPWWNELSECGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           KPKMGDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV+EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of Moc01g29410 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-120
Identity = 211/288 (73.26%), Postives = 247/288 (85.76%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MAK R SRF ARK S+   ++F +L M T V+L+LLA G+ S+P N+ +S    DLS   
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPI-DLSYFR 60

Query: 61  RKTSDDIE--EEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSET 120
           R  ++  E   ++G+QW EV+SWEPRAFVYHNFL+KEECEYL+SLAKP+M KSTVVDSET
Sbjct: 61  RAATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSET 120

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHF 180
           GKSKDSRVRTSSGTFL RGRDK I+ IEKRIAD++FIP +HGEGLQVLHYE GQKYEPH+
Sbjct: 121 GKSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHY 180

Query: 181 DYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSV 240
           DYF+D+FNTKNGGQR+AT+LMYLS+VEEGGETVFPAA  N SSVPW+NELSECGKKGLSV
Sbjct: 181 DYFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSV 240

Query: 241 KPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           KP+MGDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 KPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of Moc01g29410 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-120
Identity = 208/281 (74.02%), Postives = 244/281 (86.83%), Query Frame = 0


Query: 8   RFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  + +S K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  DIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSETGKSKDSR 127
             EE  GE+WVEVISWEPRA VYHNFLT EECE+L+SLAKP+M KSTVVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHFDYFLDDF 187
           VRTSSGTFL RG D+ +  IEKRI+DF+FIP+E+GEGLQVLHY+VGQKYEPH+DYFLD+F
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLSVKPKMGDA 247
           NTKNGGQRIATVLMYLS+V++GGETVFPAA+GNIS+VPWWNELS+CGK+GLSV PK  DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEYK 287
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V+E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of Moc01g29410 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 404.8 bits (1039), Expect = 5.7e-113
Identity = 198/288 (68.75%), Postives = 235/288 (81.60%), Query Frame = 0


Query: 3   KHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  +  S    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSE 122
              R++  D E+  G++W+EVISWEPRAFVYHNFLT EECE+L+SLAKP+M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+ +  IE RI+DF+FIP E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKGLS 242
            DYF D+FN + GGQRIATVLMYLS+V+EGGETVFPAAKGN+S VPWW+ELS+CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKMGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVNEY 286
           V PK  DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V+EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of Moc01g29410 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 2.6e-97
Identity = 176/237 (74.26%), Postives = 205/237 (86.50%), Query Frame = 0


Query: 1   MAKHRQSRFPARKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGDSPKAHDLSSIV 60
           MA+ R  R P+ + SS STL+F +LIM TFVILILLA GILS+P N+  S KA+DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKT--SDDIEEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLVSLAKPNMQKSTVVDSET 120
           RKT      ++ K E+WVE+ISWEPRA VYHNFL  EEC+YL+ LAKP+M+KSTVVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKTIRNIEKRIADFSFIPMEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDKTIR IEKRI+DF+FIP+EHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDDFNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNISSVPWWNELSECGKKG 236
           DYF+D++NT+NGGQRIATVLMYLS+VEEGGETVFPAAKGN S+VPWWNELSECGK G
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:

BLAST of Moc01g29410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133302.1	2.1e-162	100.00	probable prolyl 4-hydroxylase 10 [Momordica charantia]	[more]
XP_038881910.1	6.2e-154	94.06	probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida]	[more]
XP_008440878.1	5.2e-153	92.68	PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo]	[more]
XP_004134841.1	8.9e-153	92.33	probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prol...	[more]
XP_038881909.1	9.9e-152	92.41	probable prolyl 4-hydroxylase 10 isoform X1 [Benincasa hispida]	[more]

BLAST of Moc01g29410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JZ24	5.5e-129	77.43	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q9LN20	1.8e-119	73.26	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
Q24JN5	1.8e-119	74.02	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
F4JNU8	8.0e-112	68.75	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q8LAN3	1.6e-64	55.19	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of Moc01g29410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWA6	1.0e-162	100.00	probable prolyl 4-hydroxylase 10 OS=Momordica charantia OX=3673 GN=LOC111005915 ...	[more]
A0A1S3B1P6	2.5e-153	92.68	probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV...	[more]
A0A0A0KJH9	4.3e-153	92.33	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1EFG6	3.1e-151	91.99	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 P...	[more]
A0A6J1IRF1	6.9e-151	91.64	probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=...	[more]

BLAST of Moc01g29410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66060.1	3.9e-130	77.43	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	1.3e-120	73.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	1.3e-120	74.02	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	5.7e-113	68.75	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	2.6e-97	74.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 83..281 e-value: 4.8E-67 score: 238.7
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 163..281 e-value: 1.4E-21 score: 77.2
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 75..282 e-value: 7.9E-81 score: 272.7
None	No IPR available	PANTHER	PTHR10869:SF157	PROLYL 4-HYDROXYLASE 10-RELATED	coord: 1..286
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..286
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 159..282 score: 12.542912

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g29410.1	Moc01g29410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen