Moc01g28500 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g28500
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Location	chr1: 20236996 .. 20237900 (+)
RNA-Seq Expression	Moc01g28500
Synteny	Moc01g28500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAAACACCTCCAACTGGTAAGTAAATAGCTCGAAGTTTGAATAGCATCATCATCCGGAACTCTTCCATCCAACATCTTTCGATTCCTCGTTCCAACGCCAATTTTTATGGCAGGCTTTTCCCTTCTACAAGCTTGCATTTTGTTTATTCTTCCTACAGCATCCATTGGCTTAATTCCCAGGTCAAATCTACTGTCCTAGCCTAATTAATTGGGTGTAATTAAAAAATAATCCCATTTAAAAAATAAAGACCTGACAAATATTTATAAAATACCTAGATTATTCATCTAATTGCTAATTGATTTTGAGATATAAATCCATAATATTAAAATAGTATCCGTGTCCAACAAGTTCTTCAAACGAGCATCTAGTCAGAAAATAGGATATGAGTTGAGATTGGATCCATTCGAGAGTATGTCAAAGAATCTCACGTTGAAAAGAAAAGTAATCTAACAAATGAAAATGGAATCATATCATTGCTAGGTTCCCGAAGGGGCTTGAAAGCAATAAAGGGAGCATCTTCATATCGAATGCAAGCCCGAAGGCAGTTCTGGAAGCCTACCGAAAGCAATACCAAAAGGATTTTTCAGTGTTTCTGAAATGTCGTGCTGAGGAATTGGTGATTGGTGGGCGTATGGTTCTCATAAGTACAGTGAGAAAAAGCGAAGATAGATCCAACAAGGAGTGGTGCAATACTAATTGGGAGCTTCTCAATTTGGTTCTCAATTCCATGGTCACAGAGGTACAAATTAAACTTCTCGATTAATCCCATCTTTCAACATTCTTTTGAGTAGAATTTTGAACCTCCTTTTGCATTGGTTTGTTACAGGGAGTTGTGGAAGAGGAGAAGGTTGACTCGTTCAATGTTCCAGCCTATATACGCCATCTATGTCAGAAGTGA

mRNA sequence

ATGGAGAAAACACCTCCAACTGGCTTTTCCCTTCTACAAGCTTGCATTTTGTTTATTCTTCCTACAGCATCCATTGGCTTAATTCCCAGGTTCCCGAAGGGGCTTGAAAGCAATAAAGGGAGCATCTTCATATCGAATGCAAGCCCGAAGGCAGTTCTGGAAGCCTACCGAAAGCAATACCAAAAGGATTTTTCAGTGTTTCTGAAATGTCGTGCTGAGGAATTGGTGATTGGTGGGCGTATGGTTCTCATAAGTACAGTGAGAAAAAGCGAAGATAGATCCAACAAGGAGTGGTGCAATACTAATTGGGAGCTTCTCAATTTGGTTCTCAATTCCATGGTCACAGAGGGAGTTGTGGAAGAGGAGAAGGTTGACTCGTTCAATGTTCCAGCCTATATACGCCATCTATGTCAGAAGTGA

Coding sequence (CDS)

ATGGAGAAAACACCTCCAACTGGCTTTTCCCTTCTACAAGCTTGCATTTTGTTTATTCTTCCTACAGCATCCATTGGCTTAATTCCCAGGTTCCCGAAGGGGCTTGAAAGCAATAAAGGGAGCATCTTCATATCGAATGCAAGCCCGAAGGCAGTTCTGGAAGCCTACCGAAAGCAATACCAAAAGGATTTTTCAGTGTTTCTGAAATGTCGTGCTGAGGAATTGGTGATTGGTGGGCGTATGGTTCTCATAAGTACAGTGAGAAAAAGCGAAGATAGATCCAACAAGGAGTGGTGCAATACTAATTGGGAGCTTCTCAATTTGGTTCTCAATTCCATGGTCACAGAGGGAGTTGTGGAAGAGGAGAAGGTTGACTCGTTCAATGTTCCAGCCTATATACGCCATCTATGTCAGAAGTGA

Protein sequence

MEKTPPTGFSLLQACILFILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVIGGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAYIRHLCQK

Homology

[Close]

BLAST of Moc01g28500 vs. NCBI nr
Match: XP_022133132.1 (salicylate carboxymethyltransferase-like [Momordica charantia])

HSP 1 Score: 152.9 bits (385), Expect = 2.0e-33
Identity = 74/116 (63.79%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P+G+E+NKG+IFIS  SPK VLEAY KQ+QKDFS+FLKCRAEELV 
Sbjct: 147 FVHSSNSLHWLSQVPEGIENNKGNIFISETSPKTVLEAYHKQFQKDFSLFLKCRAEELVA 206

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAYI 134
           GGRMVL  + R  EDR NKE C  +WELLNL +N MV+EG+VEEEK+DSFNVP Y+
Sbjct: 207 GGRMVLTISARTCEDRVNKECC-YSWELLNLAVNDMVSEGIVEEEKLDSFNVPIYM 261

[Close]

BLAST of Moc01g28500 vs. NCBI nr
Match: XP_022950796.1 (salicylate carboxymethyltransferase-like [Cucurbita moschata])

HSP 1 Score: 151.8 bits (382), Expect = 4.5e-33
Identity = 75/126 (59.52%), Postives = 95/126 (75.40%), Query Frame = 0


Query: 13  QACILFILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRA 72
           +A + F+  + S+  + + P+G+ESNKG+IFIS+ SP AV EAYR Q+QKDFS FLKCRA
Sbjct: 143 RATLHFVHSSYSLHWLSQVPEGIESNKGNIFISDTSPNAVQEAYRCQFQKDFSTFLKCRA 202

Query: 73  EELVIGGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 132
           EELV+GGRMVL S+ R  EDR NKE C   WE LNL L  MV+EGVVE+EK+DSFN+P Y
Sbjct: 203 EELVVGGRMVLTSSARTCEDRVNKECCYA-WEFLNLALIDMVSEGVVEKEKMDSFNIPVY 262

Query: 133 IRHLCQ 139
           +  L +
Sbjct: 263 MPSLAE 267

[Close]

BLAST of Moc01g28500 vs. NCBI nr
Match: KAG6603952.1 (Salicylate carboxymethyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 151.8 bits (382), Expect = 4.5e-33
Identity = 75/126 (59.52%), Postives = 95/126 (75.40%), Query Frame = 0


Query: 13  QACILFILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRA 72
           +A + F+  + S+  + + P+G+ESNKG+IFIS+ SP AV EAYR Q+QKDFS FLKCRA
Sbjct: 143 RATLHFVHSSYSLHWLSQVPEGIESNKGNIFISDTSPNAVQEAYRCQFQKDFSTFLKCRA 202

Query: 73  EELVIGGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 132
           EELV+GGRMVL S+ R  EDR NKE C   WE LNL L  MV+EGVVE+EK+DSFN+P Y
Sbjct: 203 EELVVGGRMVLTSSARTCEDRVNKECCYA-WEFLNLALIDMVSEGVVEKEKMDSFNIPVY 262

Query: 133 IRHLCQ 139
           +  L +
Sbjct: 263 MPSLAE 267

[Close]

BLAST of Moc01g28500 vs. NCBI nr
Match: KAG6603955.1 (Salicylate carboxymethyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 149.4 bits (376), Expect = 2.2e-32
Identity = 77/114 (67.54%), Postives = 91/114 (79.82%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + SI  + + P GLE NKG+IF S+ SP   LEAY+KQ+QKDFS FLKCRAEELV+
Sbjct: 144 FVFSSYSIHWLSQVPDGLECNKGNIFASHRSPNQALEAYQKQHQKDFSTFLKCRAEELVV 203

Query: 78  GGRMVLISTVRKSED-RSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVP 131
           GGRMVLISTVR S+D R +KE C T WELLNL LNSMV EGV+EEEKV+SFN+P
Sbjct: 204 GGRMVLISTVRISDDRRMSKEVCYT-WELLNLALNSMVAEGVIEEEKVNSFNLP 256

[Close]

BLAST of Moc01g28500 vs. NCBI nr
Match: XP_022133143.1 (salicylate carboxymethyltransferase-like [Momordica charantia])

HSP 1 Score: 146.7 bits (369), Expect = 1.4e-31
Identity = 72/121 (59.50%), Postives = 91/121 (75.21%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P+G+ESNKG+IFIS  SPK V+EAY KQ+QKDFS FLKCRAEELV 
Sbjct: 146 FVHSSYSLHWLSQVPEGIESNKGNIFISETSPKTVVEAYHKQFQKDFSTFLKCRAEELVG 205

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAYIRHLC 137
           GGRMV+ S+ R  E+R NKE C   WE LNL LN +V+EG+V EEK+DSFNVP Y+  L 
Sbjct: 206 GGRMVVTSSARICEERVNKECCYA-WEFLNLALNDIVSEGIVGEEKLDSFNVPIYMPSLS 265

Query: 138 Q 139
           +
Sbjct: 266 E 265

[Close]

BLAST of Moc01g28500 vs. ExPASy Swiss-Prot
Match: Q9SPV4 (Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-28
Identity = 59/115 (51.30%), Postives = 87/115 (75.65%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           FI  + S+  + + P G+ESNKG+I+++N  P++VL AY KQ+Q+D ++FL+CRA+E+V 
Sbjct: 142 FIHSSYSLMWLSQVPIGIESNKGNIYMANTCPQSVLNAYYKQFQEDHALFLRCRAQEVVP 201

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           GGRMVL    R+SEDR++ E C   W+LL + LN MV+EG++EEEK+D FN+P Y
Sbjct: 202 GGRMVLTILGRRSEDRASTECC-LIWQLLAMALNQMVSEGLIEEEKMDKFNIPQY 255

[Close]

BLAST of Moc01g28500 vs. ExPASy Swiss-Prot
Match: Q84UB5 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1 OS=Petunia hybrida OX=4102 GN=BSMT1 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 57/115 (49.57%), Postives = 85/115 (73.91%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P G+E+NKG+I+++  SP +V++AY KQY+ DFS FLK R+EEL+ 
Sbjct: 148 FVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEELMK 207

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           GG+MVL    R+SED ++KE C   WELL + LN +V EG+++EEKVD+FN+P Y
Sbjct: 208 GGKMVLTLLGRESEDPTSKECCYI-WELLAMALNKLVEEGLIKEEKVDAFNIPQY 261

[Close]

BLAST of Moc01g28500 vs. ExPASy Swiss-Prot
Match: Q84UB4 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2 OS=Petunia hybrida OX=4102 GN=BSMT2 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 57/115 (49.57%), Postives = 85/115 (73.91%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P G+E+NKG+I+++  SP +V++AY KQY+ DFS FLK R+EEL+ 
Sbjct: 148 FVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEELMK 207

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           GG+MVL    R+SED ++KE C   WELL + LN +V EG+++EEKVD+FN+P Y
Sbjct: 208 GGKMVLTLLGRESEDPTSKECCYI-WELLAMALNKLVEEGLIKEEKVDAFNIPQY 261

[Close]

BLAST of Moc01g28500 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 57/115 (49.57%), Postives = 85/115 (73.91%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P G+E+NKG+I+++  SP +V++AY KQY+ DFS FLK R+EEL+ 
Sbjct: 148 FVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEELMK 207

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           GG+MVL    R+SED ++KE C   WELL + LN +V EG+++EEKVD+FN+P Y
Sbjct: 208 GGKMVLTLLGRESEDPTSKECCYI-WELLAMALNELVKEGLIKEEKVDAFNIPQY 261

[Close]

BLAST of Moc01g28500 vs. ExPASy Swiss-Prot
Match: A0A061FBW2 (Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 GN=TCM_034091 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-23
Identity = 57/121 (47.11%), Postives = 83/121 (68.60%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLES------NKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCR 77
           F+  ++S+  + + P GLES      NKG ++IS +SP++VL AY  Q+Q DFS+F++ R
Sbjct: 148 FVHSSSSLHWLSQVPPGLESKALAPLNKGKVYISKSSPQSVLNAYSLQFQNDFSMFIESR 207

Query: 78  AEELVIGGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPA 133
           ++ELV GGRMVL    R+S D + +E C+ +WELL   + S+V EG++EE KVDSFN P 
Sbjct: 208 SQELVPGGRMVLSFMGRRSTDPTTEESCH-HWELLAQAIMSLVREGLIEEAKVDSFNAPY 267

[Close]

BLAST of Moc01g28500 vs. ExPASy TrEMBL
Match: A0A6J1BVR9 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005805 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.8e-34
Identity = 74/116 (63.79%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P+G+E+NKG+IFIS  SPK VLEAY KQ+QKDFS+FLKCRAEELV 
Sbjct: 147 FVHSSNSLHWLSQVPEGIENNKGNIFISETSPKTVLEAYHKQFQKDFSLFLKCRAEELVA 206

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAYI 134
           GGRMVL  + R  EDR NKE C  +WELLNL +N MV+EG+VEEEK+DSFNVP Y+
Sbjct: 207 GGRMVLTISARTCEDRVNKECC-YSWELLNLAVNDMVSEGIVEEEKLDSFNVPIYM 261

[Close]

BLAST of Moc01g28500 vs. ExPASy TrEMBL
Match: A0A6J1GFT4 (salicylate carboxymethyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453794 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.2e-33
Identity = 75/126 (59.52%), Postives = 95/126 (75.40%), Query Frame = 0


Query: 13  QACILFILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRA 72
           +A + F+  + S+  + + P+G+ESNKG+IFIS+ SP AV EAYR Q+QKDFS FLKCRA
Sbjct: 143 RATLHFVHSSYSLHWLSQVPEGIESNKGNIFISDTSPNAVQEAYRCQFQKDFSTFLKCRA 202

Query: 73  EELVIGGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 132
           EELV+GGRMVL S+ R  EDR NKE C   WE LNL L  MV+EGVVE+EK+DSFN+P Y
Sbjct: 203 EELVVGGRMVLTSSARTCEDRVNKECCYA-WEFLNLALIDMVSEGVVEKEKMDSFNIPVY 262

Query: 133 IRHLCQ 139
           +  L +
Sbjct: 263 MPSLAE 267

[Close]

BLAST of Moc01g28500 vs. ExPASy TrEMBL
Match: A0A6J1BY84 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005817 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 7.0e-32
Identity = 72/121 (59.50%), Postives = 91/121 (75.21%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P+G+ESNKG+IFIS  SPK V+EAY KQ+QKDFS FLKCRAEELV 
Sbjct: 146 FVHSSYSLHWLSQVPEGIESNKGNIFISETSPKTVVEAYHKQFQKDFSTFLKCRAEELVG 205

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAYIRHLC 137
           GGRMV+ S+ R  E+R NKE C   WE LNL LN +V+EG+V EEK+DSFNVP Y+  L 
Sbjct: 206 GGRMVVTSSARICEERVNKECCYA-WEFLNLALNDIVSEGIVGEEKLDSFNVPIYMPSLS 265

Query: 138 Q 139
           +
Sbjct: 266 E 265

[Close]

BLAST of Moc01g28500 vs. ExPASy TrEMBL
Match: A0A5J5BXP3 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_003730 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.5e-29
Identity = 65/115 (56.52%), Postives = 92/115 (80.00%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           FI  + S+  + + P+G++ NKG+I++++ SP +VL+AY +Q+Q+DFS+FLKCR+EELV+
Sbjct: 148 FIHSSYSLQWLSKVPEGIKGNKGNIYMASTSPPSVLKAYYEQFQRDFSLFLKCRSEELVV 207

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           GGRMVL    RKSED S+KE C   WELL +VLN MV+EG++EEEK+DSFN+P Y
Sbjct: 208 GGRMVLTILGRKSEDPSSKECCYI-WELLAIVLNEMVSEGIIEEEKMDSFNIPQY 261

[Close]

BLAST of Moc01g28500 vs. ExPASy TrEMBL
Match: A0A2C9WKR2 (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G138500 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-29
Identity = 64/115 (55.65%), Postives = 90/115 (78.26%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + P+GLE NKG+I+I ++SP +VL+AY +Q+Q+DFS+FL CRA+ELV 
Sbjct: 148 FVHSSYSLHWLSQVPEGLEDNKGNIYIGSSSPLSVLKAYYRQFQRDFSLFLNCRADELVA 207

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           GGRMVL    R+S D S+KE C+  WELL +VLN+MV EG++EEEK+DSFN+P Y
Sbjct: 208 GGRMVLTFLGRRSPDPSSKECCHYIWELLAMVLNNMVLEGIIEEEKLDSFNIPNY 262

[Close]

BLAST of Moc01g28500 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 99.0 bits (245), Expect = 3.2e-21
Identity = 51/95 (53.68%), Postives = 64/95 (67.37%), Query Frame = 0


Query: 38  NKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVIGGRMVLISTVRKSEDRSNKE 97
           N G I+IS  SPK+  +AY  Q+Q DF VFL+ R+EELV GGRMVL    R+S D + +E
Sbjct: 186 NMGKIYISKTSPKSAHKAYALQFQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLDPTTEE 245

Query: 98  WCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
            C   WELL   L SM  EG++EEEK+D+FN P Y
Sbjct: 246 SC-YQWELLAQALMSMAKEGIIEEEKIDAFNAPYY 279

[Close]

BLAST of Moc01g28500 vs. TAIR 10
Match: AT5G66430.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 2.1e-20
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  + S+  + + PKGLE N  S++I+ +SP    +AY  Q+Q DF  FL+ R+EE+V 
Sbjct: 136 FVHSSYSLHWLSKVPKGLEKNSSSVYITTSSPPNAYKAYLNQFQSDFKSFLEMRSEEMVS 195

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
            GRMVL    RK+ D      C   W LL+  L  +V EG+V   KVDSFN+P Y
Sbjct: 196 NGRMVLTFIGRKTLDDPLHRDCCHFWTLLSTSLRDLVYEGLVSASKVDSFNIPFY 250

[Close]

BLAST of Moc01g28500 vs. TAIR 10
Match: AT5G04370.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 2.7e-20
Identity = 48/115 (41.74%), Postives = 70/115 (60.87%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  +  +  + + P+GLE NK S++I+N+SP +  +AY  Q+Q+DF+ FLK R+EE+V 
Sbjct: 177 FVHSSYGLHWLSKVPEGLEKNKMSVYITNSSPLSTYKAYLNQFQRDFATFLKLRSEEMVS 236

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
            GRMVL    R + D      C   W LL+  L  +V EG+V   KVDSF +P Y
Sbjct: 237 NGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAEGLVSASKVDSFYLPFY 291

[Close]

BLAST of Moc01g28500 vs. TAIR 10
Match: AT5G04370.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 2.7e-20
Identity = 48/115 (41.74%), Postives = 70/115 (60.87%), Query Frame = 0


Query: 18  FILPTASIGLIPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVI 77
           F+  +  +  + + P+GLE NK S++I+N+SP +  +AY  Q+Q+DF+ FLK R+EE+V 
Sbjct: 196 FVHSSYGLHWLSKVPEGLEKNKMSVYITNSSPLSTYKAYLNQFQRDFATFLKLRSEEMVS 255

Query: 78  GGRMVLISTVRKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
            GRMVL    R + D      C   W LL+  L  +V EG+V   KVDSF +P Y
Sbjct: 256 NGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAEGLVSASKVDSFYLPFY 310

[Close]

BLAST of Moc01g28500 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-19
Identity = 44/105 (41.90%), Postives = 71/105 (67.62%), Query Frame = 0


Query: 28  IPRFPKGLESNKGSIFISNASPKAVLEAYRKQYQKDFSVFLKCRAEELVIGGRMVLISTV 87
           + + P+ LE+NKG+++I+++SP++  +AY  Q+QKDF++FL+ R+EE+V  GRMVL    
Sbjct: 178 LSKVPEKLENNKGNLYITSSSPQSAYKAYLNQFQKDFTMFLRLRSEEIVSNGRMVLTFIG 237

Query: 88  RKSEDRSNKEWCNTNWELLNLVLNSMVTEGVVEEEKVDSFNVPAY 133
           R + +      C   W LL+  L  +V EG+V E K+D+FN+P Y
Sbjct: 238 RNTLNDPLYRDCCHFWTLLSNSLRDLVFEGLVSESKLDAFNMPFY 282

The following BLAST results are available for this feature:

BLAST of Moc01g28500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133132.1	2.0e-33	63.79	salicylate carboxymethyltransferase-like [Momordica charantia]	[more]
XP_022950796.1	4.5e-33	59.52	salicylate carboxymethyltransferase-like [Cucurbita moschata]	[more]
KAG6603952.1	4.5e-33	59.52	Salicylate carboxymethyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]
KAG6603955.1	2.2e-32	67.54	Salicylate carboxymethyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022133143.1	1.4e-31	59.50	salicylate carboxymethyltransferase-like [Momordica charantia]	[more]

BLAST of Moc01g28500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SPV4	4.5e-28	51.30	Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=...	[more]
Q84UB5	2.5e-26	49.57	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1...	[more]
Q84UB4	2.5e-26	49.57	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2...	[more]
A4ZDG8	2.5e-26	49.57	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3...	[more]
A0A061FBW2	1.5e-23	47.11	Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 G...	[more]

BLAST of Moc01g28500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVR9	9.8e-34	63.79	salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1GFT4	2.2e-33	59.52	salicylate carboxymethyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1BY84	7.0e-32	59.50	salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5J5BXP3	1.5e-29	56.52	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_003730 PE=3 SV=1	[more]
A0A2C9WKR2	2.5e-29	55.65	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G138500 PE=4 SV=...	[more]

BLAST of Moc01g28500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19640.1	3.2e-21	53.68	jasmonic acid carboxyl methyltransferase	[more]
AT5G66430.1	2.1e-20	42.61	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G04370.1	2.7e-20	41.74	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G04370.2	2.7e-20	41.74	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G11480.1	1.8e-19	41.90	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005299	SAM dependent carboxyl methyltransferase	PFAM	PF03492	Methyltransf_7	coord: 30..135 e-value: 3.3E-37 score: 128.5
IPR005299	SAM dependent carboxyl methyltransferase	PANTHER	PTHR31009	S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN	coord: 24..135
IPR042086	Methyltransferase, alpha-helical capping domain	GENE3D	1.10.1200.270		coord: 30..129 e-value: 6.3E-39 score: 135.8
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 16..135 e-value: 6.3E-39 score: 135.8
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..15 score: 5.0
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 20..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g28500.1	Moc01g28500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity