Moc01g27870 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g27870
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cytochrome P450 CYP736A12-like
Location	chr1: 19813139 .. 19813645 (+)
RNA-Seq Expression	Moc01g27870
Synteny	Moc01g27870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTGGGTTTGGTTCATTATTACAACACTTCCTCTATTTCTTCTCTTCAGCTCGTGGCTATGGGTGCTCAGAAGCCACGCCAGATTGCCTCTTGGTCCTAAAGGATTTCCAATTTTTGGAAGCCTTCATTTGTTAGGAAAGCTTCCACATAGAGCTCTCCATCGCCTATCTCAAAAGTATGGCCCCATTATGCACTTGCGATTAGGTCTTGTACCCACCATCGTAGTCTCTTCTCCTGCCACCGCCGAGCTCTTTCTCAAAACCTACGACCATGTCTTCGCCAGCCGCCCACTCATCCAGGCCTCCAACCACATTTCTTACGGTCGAAAGTGTATGGGGTTCGCTGAATATGGGCCTTATTGGCGCAATATTCGAAAAATGTGCACTCTCGAGTTGCTTAGCAATCTCAAAGTTAGCTCCTTTAGGTCAATGAGAAGCGAGGAGCTTGGGTTGTTGATCGACCATCTTCGAGAGGCTGCAAAAAGTGATTTAAATACCCCATAA

mRNA sequence

ATGGCTTGGGTTTGGTTCATTATTACAACACTTCCTCTATTTCTTCTCTTCAGCTCGTGGCTATGGGTGCTCAGAAGCCACGCCAGATTGCCTCTTGGTCCTAAAGGATTTCCAATTTTTGGAAGCCTTCATTTGTTAGGAAAGCTTCCACATAGAGCTCTCCATCGCCTATCTCAAAAGTATGGCCCCATTATGCACTTGCGATTAGGTCTTGTACCCACCATCGTAGTCTCTTCTCCTGCCACCGCCGAGCTCTTTCTCAAAACCTACGACCATGTCTTCGCCAGCCGCCCACTCATCCAGGCCTCCAACCACATTTCTTACGGTCGAAAGTGTATGGGGTTCGCTGAATATGGGCCTTATTGGCGCAATATTCGAAAAATGTGCACTCTCGAGTTGCTTAGCAATCTCAAAGTTAGCTCCTTTAGGTCAATGAGAAGCGAGGAGCTTGGGTTGTTGATCGACCATCTTCGAGAGGCTGCAAAAAGTGATTTAAATACCCCATAA

Coding sequence (CDS)

ATGGCTTGGGTTTGGTTCATTATTACAACACTTCCTCTATTTCTTCTCTTCAGCTCGTGGCTATGGGTGCTCAGAAGCCACGCCAGATTGCCTCTTGGTCCTAAAGGATTTCCAATTTTTGGAAGCCTTCATTTGTTAGGAAAGCTTCCACATAGAGCTCTCCATCGCCTATCTCAAAAGTATGGCCCCATTATGCACTTGCGATTAGGTCTTGTACCCACCATCGTAGTCTCTTCTCCTGCCACCGCCGAGCTCTTTCTCAAAACCTACGACCATGTCTTCGCCAGCCGCCCACTCATCCAGGCCTCCAACCACATTTCTTACGGTCGAAAGTGTATGGGGTTCGCTGAATATGGGCCTTATTGGCGCAATATTCGAAAAATGTGCACTCTCGAGTTGCTTAGCAATCTCAAAGTTAGCTCCTTTAGGTCAATGAGAAGCGAGGAGCTTGGGTTGTTGATCGACCATCTTCGAGAGGCTGCAAAAAGTGATTTAAATACCCCATAA

Protein sequence

MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKSDLNTP

Homology

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BLAST of Moc01g27870 vs. NCBI nr
Match: XP_022132564.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 307.8 bits (787), Expect = 5.9e-80
Identity = 153/165 (92.73%), Postives = 158/165 (95.76%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MA VW IIT LPLFLLFSSWLWVLRSHARLP GPKGFPIFGSLHLLGKLPHRALHRLSQK
Sbjct: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YGPIMHLRLGLV TIVVSSPA AELFLKT+DHVFASRPLIQASNHISYGRK +GFAEYGP
Sbjct: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKSDL 166
           YWRNIRKMCT+ELLSNLKVSSFRSMRSEE+GLLIDHLREAAKSD+
Sbjct: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDV 165

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BLAST of Moc01g27870 vs. NCBI nr
Match: XP_022133199.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 222.6 bits (566), Expect = 2.5e-54
Identity = 110/164 (67.07%), Postives = 130/164 (79.27%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MAW+W  + +L   LL S          +LP GPKGFPIFGSLHLLGKLPHR L +LSQ+
Sbjct: 1   MAWIWVTLVSLIALLLRSCLFKNHNKRKKLPPGPKGFPIFGSLHLLGKLPHRDLQKLSQE 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YGPIMH++LGLVPTI+VSSP+ AELFLKT+D VFASRP  +AS HISY +K + F+ YGP
Sbjct: 61  YGPIMHMKLGLVPTIIVSSPSAAELFLKTHDLVFASRPPSEASKHISYRQKNLVFSPYGP 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKSD 165
           YWRN+RKMCTLELLSNLK++SF+SMR  +LGLLI HLREAA  D
Sbjct: 121 YWRNMRKMCTLELLSNLKINSFKSMRKHQLGLLIQHLREAAACD 164

[Close]

BLAST of Moc01g27870 vs. NCBI nr
Match: XP_022978478.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 221.5 bits (563), Expect = 5.6e-54
Identity = 112/163 (68.71%), Postives = 132/163 (80.98%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MAW+   I  L + L F+SWL   R   +LP GP+G PI GSLHLLGKLPHR L  LSQK
Sbjct: 1   MAWILLAIALLGIALFFTSWLH--RGPNQLPPGPRGLPILGSLHLLGKLPHRDLRALSQK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YG IM++RLGLVPTIVVSSP  AELFLKT+D VFASRP +QAS ++SYG+K + FA YG 
Sbjct: 61  YGSIMYMRLGLVPTIVVSSPQAAELFLKTHDIVFASRPFVQASKYMSYGQKNLAFAPYGS 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           YWRN+RKMCTLELLSN+KV+SFRSMR EELGLL+D+LR+AAK+
Sbjct: 121 YWRNMRKMCTLELLSNVKVNSFRSMRREELGLLVDYLRDAAKN 161

[Close]

BLAST of Moc01g27870 vs. NCBI nr
Match: XP_015867271.1 (cytochrome P450 CYP736A12-like [Ziziphus jujuba])

HSP 1 Score: 221.1 bits (562), Expect = 7.3e-54
Identity = 108/164 (65.85%), Postives = 134/164 (81.71%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVL--RSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLS 60
           MAW+W   T L L LL  + LW +   ++ RLP GP+GFPIFGSLHLLG+ PHR LHRL+
Sbjct: 1   MAWIW---TILALLLLAYTLLWKITNNNNKRLPPGPRGFPIFGSLHLLGEFPHRDLHRLA 60

Query: 61  QKYGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEY 120
           ++YGPIMH+RLGL+PTIVVSSP  AELFLKT+D VFASRP  +A+ HISY +K M FAEY
Sbjct: 61  KQYGPIMHIRLGLIPTIVVSSPKAAELFLKTHDLVFASRPPHEAAKHISYNQKSMAFAEY 120

Query: 121 GPYWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAK 163
           G YWR++RKMCTLELLSN+K++SFR+MR EE+GL ID LR++A+
Sbjct: 121 GSYWRDVRKMCTLELLSNVKINSFRAMRKEEVGLFIDSLRKSAR 161

[Close]

BLAST of Moc01g27870 vs. NCBI nr
Match: XP_008440675.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >KAA0036257.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa] >TYK12651.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 221.1 bits (562), Expect = 7.3e-54
Identity = 108/162 (66.67%), Postives = 135/162 (83.33%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MAW++  +  + L +L  + L+  RS  +LP GP+GFPIFG LHLLGKLPHR L  LS+K
Sbjct: 1   MAWIFLAVALVGLAILLRTRLY--RSPNKLPPGPRGFPIFGCLHLLGKLPHRDLRSLSKK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YG IM++RLGLVPT++VSSP  AELFLKT+D VFASRP +QAS ++SYG+K +GFA+YGP
Sbjct: 61  YGSIMYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGP 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAK 163
           YWRN+RKMCTLELLS++KV SFRSMR EELGLL+D+LR+AAK
Sbjct: 121 YWRNMRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAK 160

[Close]

BLAST of Moc01g27870 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.6e-48
Identity = 95/163 (58.28%), Postives = 124/163 (76.07%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           M W+W  I  L L  +  +W W      RLP GP+GFPIFGSLHLLG+ P++ LHRL++K
Sbjct: 1   MVWIWATIGLLALVHILQAW-W-KNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YG IM++RLGL+PTIV+SSP  AELFLKT+D VFASRP  + S HIS+G+K + F+EYG 
Sbjct: 61  YGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGA 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           YWR+ RKMCT+ELLSN K++SF+SMR EE+ L ++ +R AA +
Sbjct: 121 YWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANN 161

[Close]

BLAST of Moc01g27870 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.1e-43
Identity = 87/159 (54.72%), Postives = 113/159 (71.07%), Query Frame = 0


Query: 13  LFLLFSSWLW-------VLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIM 72
           LF+L  +  W        L+ H +LP GP+G PI GSLH LG LPHR L  L++KYGPIM
Sbjct: 9   LFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIM 68

Query: 73  HLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNI 132
            +RLG VPTIVVSSP  AELFLKT+D++FASRP +QA+ ++SYG   M F  YGP+WRNI
Sbjct: 69  SMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNI 128

Query: 133 RKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKSD 165
           RK   LELL+  K++SF  MR EELG ++  ++EA+ ++
Sbjct: 129 RKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAAN 167

[Close]

BLAST of Moc01g27870 vs. ExPASy Swiss-Prot
Match: Q9LTM1 (Cytochrome P450 71B22 OS=Arabidopsis thaliana OX=3702 GN=CYP71B22 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.1e-32
Identity = 72/158 (45.57%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 6   FIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIM 65
           + +  LPLFL+F   L    S  +LP GP G PI G+LH LGK  HR+ H+LSQ YGP+M
Sbjct: 6   YFLLLLPLFLIFFKKL--SPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVM 65

Query: 66  HLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNI 125
            L  G+VP +VVS+   AE  LKT+D    +RP + A+   SY  K +GFA+YG  WR +
Sbjct: 66  FLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREM 125

Query: 126 RKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           RK+  LEL S+ K+ +FR +R EE  +L++ L ++A++
Sbjct: 126 RKLAMLELFSSKKLKAFRYIREEESEVLVNKLSKSAET 161

[Close]

BLAST of Moc01g27870 vs. ExPASy Swiss-Prot
Match: O65784 (Cytochrome P450 71B5 OS=Arabidopsis thaliana OX=3702 GN=CYP71B5 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 6.9e-31
Identity = 75/158 (47.47%), Postives = 98/158 (62.03%), Query Frame = 0


Query: 11  LPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLG 70
           LPL L+F   L  L S  +LP GPKG PI G+LH  G+  H++LH++SQ+YGP+M L  G
Sbjct: 11  LPLSLIFLKKL--LPSKGKLPPGPKGLPIIGNLHQFGRFLHKSLHKISQEYGPVMLLHFG 70

Query: 71  LVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNIRKMCT 130
           +VP I+VSS   AE  LKT+D    SRP    S   +Y  K +GFA YG  WR +RK+  
Sbjct: 71  VVPVIIVSSKEGAEEVLKTHDLETCSRPKTVGSGLFTYNFKDIGFAPYGENWREMRKIAV 130

Query: 131 LELLSNLKVSSFRSMRSEELGLLIDHLREAAKSDLNTP 169
            EL S  K+ SFR +R +E  LL+   R+ +KS L TP
Sbjct: 131 SELFSQKKLKSFRYIREDESQLLV---RKVSKSALETP 163

[Close]

BLAST of Moc01g27870 vs. ExPASy Swiss-Prot
Match: Q9LTM2 (Cytochrome P450 71B21 OS=Arabidopsis thaliana OX=3702 GN=CYP71B21 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 6.9e-31
Identity = 71/153 (46.41%), Postives = 100/153 (65.36%), Query Frame = 0


Query: 11  LPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLG 70
           LPLFL+F   L  L S  +LP GP   PI G+LH LGK  HR+ ++LSQ+YGP+M LR G
Sbjct: 11  LPLFLVFYKRL--LPSKGKLPPGPISLPIIGNLHQLGKSLHRSFYKLSQEYGPVMFLRFG 70

Query: 71  LVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNIRKMCT 130
           +VP +V S+   AE  LKT+D    +RP + A+   +Y  K +GFA+YG  WR +RK+  
Sbjct: 71  VVPVVVFSTKEAAEEVLKTHDLETCTRPKLSATGLFTYNFKDIGFAQYGEDWREMRKLAM 130

Query: 131 LELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           LEL S+ K+ +FR +R EE  LL+  + E+A++
Sbjct: 131 LELFSSKKLKAFRYIREEESELLVKKVTESAQT 161

[Close]

BLAST of Moc01g27870 vs. ExPASy TrEMBL
Match: A0A6J1BTE3 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.9e-80
Identity = 153/165 (92.73%), Postives = 158/165 (95.76%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MA VW IIT LPLFLLFSSWLWVLRSHARLP GPKGFPIFGSLHLLGKLPHRALHRLSQK
Sbjct: 1   MASVWLIITILPLFLLFSSWLWVLRSHARLPPGPKGFPIFGSLHLLGKLPHRALHRLSQK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YGPIMHLRLGLV TIVVSSPA AELFLKT+DHVFASRPLIQASNHISYGRK +GFAEYGP
Sbjct: 61  YGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYGP 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKSDL 166
           YWRNIRKMCT+ELLSNLKVSSFRSMRSEE+GLLIDHLREAAKSD+
Sbjct: 121 YWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDV 165

[Close]

BLAST of Moc01g27870 vs. ExPASy TrEMBL
Match: A0A6J1BVB2 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005855 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.2e-54
Identity = 110/164 (67.07%), Postives = 130/164 (79.27%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MAW+W  + +L   LL S          +LP GPKGFPIFGSLHLLGKLPHR L +LSQ+
Sbjct: 1   MAWIWVTLVSLIALLLRSCLFKNHNKRKKLPPGPKGFPIFGSLHLLGKLPHRDLQKLSQE 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YGPIMH++LGLVPTI+VSSP+ AELFLKT+D VFASRP  +AS HISY +K + F+ YGP
Sbjct: 61  YGPIMHMKLGLVPTIIVSSPSAAELFLKTHDLVFASRPPSEASKHISYRQKNLVFSPYGP 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKSD 165
           YWRN+RKMCTLELLSNLK++SF+SMR  +LGLLI HLREAA  D
Sbjct: 121 YWRNMRKMCTLELLSNLKINSFKSMRKHQLGLLIQHLREAAACD 164

[Close]

BLAST of Moc01g27870 vs. ExPASy TrEMBL
Match: A0A6J1IT84 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478445 PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.7e-54
Identity = 112/163 (68.71%), Postives = 132/163 (80.98%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MAW+   I  L + L F+SWL   R   +LP GP+G PI GSLHLLGKLPHR L  LSQK
Sbjct: 1   MAWILLAIALLGIALFFTSWLH--RGPNQLPPGPRGLPILGSLHLLGKLPHRDLRALSQK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YG IM++RLGLVPTIVVSSP  AELFLKT+D VFASRP +QAS ++SYG+K + FA YG 
Sbjct: 61  YGSIMYMRLGLVPTIVVSSPQAAELFLKTHDIVFASRPFVQASKYMSYGQKNLAFAPYGS 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           YWRN+RKMCTLELLSN+KV+SFRSMR EELGLL+D+LR+AAK+
Sbjct: 121 YWRNMRKMCTLELLSNVKVNSFRSMRREELGLLVDYLRDAAKN 161

[Close]

BLAST of Moc01g27870 vs. ExPASy TrEMBL
Match: A0A5A7T3U2 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002390 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.5e-54
Identity = 108/162 (66.67%), Postives = 135/162 (83.33%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQK 60
           MAW++  +  + L +L  + L+  RS  +LP GP+GFPIFG LHLLGKLPHR L  LS+K
Sbjct: 1   MAWIFLAVALVGLAILLRTRLY--RSPNKLPPGPRGFPIFGCLHLLGKLPHRDLRSLSKK 60

Query: 61  YGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGP 120
           YG IM++RLGLVPT++VSSP  AELFLKT+D VFASRP +QAS ++SYG+K +GFA+YGP
Sbjct: 61  YGSIMYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGP 120

Query: 121 YWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAK 163
           YWRN+RKMCTLELLS++KV SFRSMR EELGLL+D+LR+AAK
Sbjct: 121 YWRNMRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAK 160

[Close]

BLAST of Moc01g27870 vs. ExPASy TrEMBL
Match: A0A6P3YU66 (cytochrome P450 CYP736A12-like OS=Ziziphus jujuba OX=326968 GN=LOC107404788 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.5e-54
Identity = 108/164 (65.85%), Postives = 134/164 (81.71%), Query Frame = 0


Query: 1   MAWVWFIITTLPLFLLFSSWLWVL--RSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLS 60
           MAW+W   T L L LL  + LW +   ++ RLP GP+GFPIFGSLHLLG+ PHR LHRL+
Sbjct: 1   MAWIW---TILALLLLAYTLLWKITNNNNKRLPPGPRGFPIFGSLHLLGEFPHRDLHRLA 60

Query: 61  QKYGPIMHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEY 120
           ++YGPIMH+RLGL+PTIVVSSP  AELFLKT+D VFASRP  +A+ HISY +K M FAEY
Sbjct: 61  KQYGPIMHIRLGLIPTIVVSSPKAAELFLKTHDLVFASRPPHEAAKHISYNQKSMAFAEY 120

Query: 121 GPYWRNIRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAK 163
           G YWR++RKMCTLELLSN+K++SFR+MR EE+GL ID LR++A+
Sbjct: 121 GSYWRDVRKMCTLELLSNVKINSFRAMRKEEVGLFIDSLRKSAR 161

[Close]

BLAST of Moc01g27870 vs. TAIR 10
Match: AT3G26200.1 (cytochrome P450, family 71, subfamily B, polypeptide 22 )

HSP 1 Score: 138.3 bits (347), Expect = 5.8e-33
Identity = 72/158 (45.57%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 6   FIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIM 65
           + +  LPLFL+F   L    S  +LP GP G PI G+LH LGK  HR+ H+LSQ YGP+M
Sbjct: 6   YFLLLLPLFLIFFKKL--SPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVM 65

Query: 66  HLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNI 125
            L  G+VP +VVS+   AE  LKT+D    +RP + A+   SY  K +GFA+YG  WR +
Sbjct: 66  FLHFGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREM 125

Query: 126 RKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           RK+  LEL S+ K+ +FR +R EE  +L++ L ++A++
Sbjct: 126 RKLAMLELFSSKKLKAFRYIREEESEVLVNKLSKSAET 161

[Close]

BLAST of Moc01g27870 vs. TAIR 10
Match: AT3G53280.1 (cytochrome p450 71b5 )

HSP 1 Score: 135.2 bits (339), Expect = 4.9e-32
Identity = 75/158 (47.47%), Postives = 98/158 (62.03%), Query Frame = 0


Query: 11  LPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLG 70
           LPL L+F   L  L S  +LP GPKG PI G+LH  G+  H++LH++SQ+YGP+M L  G
Sbjct: 11  LPLSLIFLKKL--LPSKGKLPPGPKGLPIIGNLHQFGRFLHKSLHKISQEYGPVMLLHFG 70

Query: 71  LVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNIRKMCT 130
           +VP I+VSS   AE  LKT+D    SRP    S   +Y  K +GFA YG  WR +RK+  
Sbjct: 71  VVPVIIVSSKEGAEEVLKTHDLETCSRPKTVGSGLFTYNFKDIGFAPYGENWREMRKIAV 130

Query: 131 LELLSNLKVSSFRSMRSEELGLLIDHLREAAKSDLNTP 169
            EL S  K+ SFR +R +E  LL+   R+ +KS L TP
Sbjct: 131 SELFSQKKLKSFRYIREDESQLLV---RKVSKSALETP 163

[Close]

BLAST of Moc01g27870 vs. TAIR 10
Match: AT3G26190.1 (cytochrome P450, family 71, subfamily B, polypeptide 21 )

HSP 1 Score: 135.2 bits (339), Expect = 4.9e-32
Identity = 71/153 (46.41%), Postives = 100/153 (65.36%), Query Frame = 0


Query: 11  LPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLG 70
           LPLFL+F   L  L S  +LP GP   PI G+LH LGK  HR+ ++LSQ+YGP+M LR G
Sbjct: 11  LPLFLVFYKRL--LPSKGKLPPGPISLPIIGNLHQLGKSLHRSFYKLSQEYGPVMFLRFG 70

Query: 71  LVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNIRKMCT 130
           +VP +V S+   AE  LKT+D    +RP + A+   +Y  K +GFA+YG  WR +RK+  
Sbjct: 71  VVPVVVFSTKEAAEEVLKTHDLETCTRPKLSATGLFTYNFKDIGFAQYGEDWREMRKLAM 130

Query: 131 LELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           LEL S+ K+ +FR +R EE  LL+  + E+A++
Sbjct: 131 LELFSSKKLKAFRYIREEESELLVKKVTESAQT 161

[Close]

BLAST of Moc01g27870 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 132.5 bits (332), Expect = 3.2e-31
Identity = 66/136 (48.53%), Postives = 84/136 (61.76%), Query Frame = 0


Query: 26  SHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPIMHLRLGLVPTIVVSSPATAEL 85
           S   LP  P   PI G+LH L  LPHR  H+LS KYGP++ LRLG VP +V+SS   AE 
Sbjct: 27  SKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPLVFLRLGSVPVVVISSSEAAEA 86

Query: 86  FLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRNIRKMCTLELLSNLKVSSFRSM 145
            LKT D    SRP    S  +SYG K + FA YG YWR +RK+  +EL S+ KV SFR +
Sbjct: 87  VLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKLAVIELFSSKKVQSFRYI 146

Query: 146 RSEELGLLIDHLREAA 162
           R EE+  ++  + E+A
Sbjct: 147 REEEVDFVVKKVSESA 162

[Close]

BLAST of Moc01g27870 vs. TAIR 10
Match: AT2G24180.1 (cytochrome p450 71b6 )

HSP 1 Score: 131.0 bits (328), Expect = 9.2e-31
Identity = 67/159 (42.14%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 5   WFIITTLPLFLLFSSWLWVLRSHARLPLGPKGFPIFGSLHLLGKLPHRALHRLSQKYGPI 64
           W ++  +P  L+F    ++LRS   LP GP   PI G++H LG LPHR+L  LS KYGP+
Sbjct: 15  WLLLLLIPPLLIF----FLLRSPKNLPPGPPRLPILGNIHQLGSLPHRSLRDLSLKYGPV 74

Query: 65  MHLRLGLVPTIVVSSPATAELFLKTYDHVFASRPLIQASNHISYGRKCMGFAEYGPYWRN 124
           + + LG V T+VV SP TAE  LK +D    +RP +  +    Y    +GF ++G Y+R+
Sbjct: 75  ITVYLGSVRTVVVHSPETAEEVLKLHDSECCTRPKLSITKSFFYDGLGLGFTKWGDYYRD 134

Query: 125 IRKMCTLELLSNLKVSSFRSMRSEELGLLIDHLREAAKS 164
           +RK+C LEL S  + +SFR++R EEL  L++   ++A S
Sbjct: 135 VRKLCVLELFSVKRANSFRNIREEELSRLVNSFSDSASS 169

The following BLAST results are available for this feature:

BLAST of Moc01g27870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132564.1	5.9e-80	92.73	cytochrome P450 CYP736A12-like [Momordica charantia]	[more]
XP_022133199.1	2.5e-54	67.07	cytochrome P450 CYP736A12-like [Momordica charantia]	[more]
XP_022978478.1	5.6e-54	68.71	cytochrome P450 CYP736A12-like [Cucurbita maxima]	[more]
XP_015867271.1	7.3e-54	65.85	cytochrome P450 CYP736A12-like [Ziziphus jujuba]	[more]
XP_008440675.1	7.3e-54	66.67	PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >KAA0036257.1 cytochrom...	[more]

BLAST of Moc01g27870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q5V6	1.6e-48	58.28	Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1	[more]
H2DH18	2.1e-43	54.72	Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
Q9LTM1	8.1e-32	45.57	Cytochrome P450 71B22 OS=Arabidopsis thaliana OX=3702 GN=CYP71B22 PE=2 SV=1	[more]
O65784	6.9e-31	47.47	Cytochrome P450 71B5 OS=Arabidopsis thaliana OX=3702 GN=CYP71B5 PE=2 SV=1	[more]
Q9LTM2	6.9e-31	46.41	Cytochrome P450 71B21 OS=Arabidopsis thaliana OX=3702 GN=CYP71B21 PE=3 SV=1	[more]

BLAST of Moc01g27870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTE3	2.9e-80	92.73	cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE...	[more]
A0A6J1BVB2	1.2e-54	67.07	cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005855 PE...	[more]
A0A6J1IT84	2.7e-54	68.71	cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478445 PE=3 ...	[more]
A0A5A7T3U2	3.5e-54	66.67	Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6P3YU66	3.5e-54	65.85	cytochrome P450 CYP736A12-like OS=Ziziphus jujuba OX=326968 GN=LOC107404788 PE=3...	[more]

BLAST of Moc01g27870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26200.1	5.8e-33	45.57	cytochrome P450, family 71, subfamily B, polypeptide 22	[more]
AT3G53280.1	4.9e-32	47.47	cytochrome p450 71b5	[more]
AT3G26190.1	4.9e-32	46.41	cytochrome P450, family 71, subfamily B, polypeptide 21	[more]
AT1G13080.1	3.2e-31	48.53	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT2G24180.1	9.2e-31	42.14	cytochrome p450 71b6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 31..164 e-value: 7.3E-18 score: 64.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..167 e-value: 4.4E-29 score: 103.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 24..162
None	No IPR available	PANTHER	PTHR24299	CYTOCHROME P450 FAMILY 1	coord: 6..161
None	No IPR available	PANTHER	PTHR24299:SF37	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 6..161

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g27870.1	Moc01g27870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen