Moc01g25940 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g25940
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr1: 18463409 .. 18467699 (+)
RNA-Seq Expression	Moc01g25940
Synteny	Moc01g25940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGGGAAAATCGGAAAAGGTAATTGCAGCTTCAAATCGAAGCTAGGTTTGCCGGCGCTCCTCTTCGTTGTATGCCTATTTTGTTTCCTCGTTGGATTCTTCGGTTCTTCTCTCTTCTCTCAGGTTGATCCGTGTGCTTCAAGTTTCTGCTTACTATTAGATTTCATCGCTTTAATTAATGCCACTGTTTTAATTTCCCAATTTTGTCATTGTCATCGGATGGATGAACAGGATGTAAATGGCGATAGGCCGAGGCCGAGGATGCTTCATTCGGTCAGCGATGGTAGCGATTACGATCCGATGCCATATGGAGAAGGCGGCGACGATTCCATTTCTTCGATTCCATTTCAGGTACTCTCCATTTGGTTTTAATTCTTATTTTGCCGTAGTTTCACCGCATTTCCACTCAAACTGAGTTGTTCATATTAGGATTGTCATGTATTTATGGGAAGTATAACTTGATCGACGAACGAACCTTGCAGTTGGCTATTCAACCTTTAGTATTGCACTTCTAATTTAAACTTTTCCCAAATGTAGGTTTTGAGTTGGCAACCACGCGCAGTTTACTTTCCTAAGTTCGCAACTGCTGAGCAATGCCAAAACATAGTTAATACGGCGAAACCGAACCTTAAACCGTCTACCTTGGCTCTCCGTAAGGGAGAAACTGAAGAGAACACGAAGGGGATCCGGACAAGGTATCTTCTGTCTCCATGTACTGCCACATATGACTTTACTGATTATATTTTATTAGTACGAATTTAATGACATTCTCAATGAGGTGATTCATAGAGTTAATGTAGTAAGAGTCGTCAGACTATGGTGATTCCGTATGAGGATGACTGCATAAAATATTTGTGCATTAGTACGTGTTATTCTCACGTTCTCTGGGATTGACATTTGGTTTTTATTTCTAATTTTCTCACCCTCTTCTTCGAACTACCCTAACCTAGGTTTCTTCCTCTATTTCCAATTATAAAGTTATGCTGAAAGACTTGCTTATTTGGTTTGAAAATTTATCCACCGAGATGTAAGATTTCAATTAACATGGTTATGTCTTAGTTTTTGCTTCTTCCTTTTCAGTTCGACTCTTTTTGTCATTCACTCTCATCTATAGCTGCTGATTGGAGTTCATTCTTAACTCCGTTGAGGGGATTTCCAATTCCATCCTCTTCTGTAGTTTCATGTTATGAATGAATTGTTTCATATCTAAAGAAATATTTGTACTTCATATTCTCTTGGCAACTATTGCACTTTTCTATTACGTTTTGTAATGGTTATACGAATTACAACCATTACAAAACTTAATAGAAAATGGACAATTTCTATTACTTTAACTTAATATTTTTCATGAATTGAAGGCTAAGGAATGCATGATCTTCTAAGTTTTTGGTTATACTCTGGTTATAGGAATATATAATTTTGTAAACGTGCTTGGTTACATTTTCAATTTTCCCTTTCTTTTCCTGTTTCCTGTCTCATGCAGTTCTGGAGTTTTCTTGAGTGCTTCAGAAGATAAAAGTGGGACTCTGGATGACATCGAGAAAAAAATTGCAAGGGCAACTATGCTTCCAAGGACGCATGGAGAGGTATTATGATAGTAAGATTTACACTTTGACCATCAGTATTTCCACCCTGCTTCTTGAAGGTGGAATTCTATTGTTATATCATGCATAAGAGTTTTAAACTTTCAAAAGCATTTGTTCTACAATCCTGAATACCTTTGATGTAAAATTTTTATAGGGAGTGGCAAGGATTGAACTCTTTCAATTCTTTGGAGAAGATGAAACCATCAGCTAGTTACCTTTAATGTCATTTTCTAACTTGCTCGTTGTTTCTTTTCCATCTCCAGTTATAGTAGTTTGGGACTTTGGGGCCTTCTAAAAGTTTAATGCTTGATGTGGGATCTTGACCATTCATTTCTTATAGTCATTGATTGGCTTTATCTAAATCTACTACGTGTCCCCTTTTAGTTAGATTTTGTTGGTTATTTTAAGCTGATATTAAAATTTTCTCAAACTTCTTGTTTCTTGTTTCTTGTTTACATTTCTTGAAGAATAAAAGTTTGACAACTCAAAAAGAGGATTAGTATTACCTCCTCACATTTTTCTTTGGGACGTAATAATAACAGGTGACAGAAACCTGATAGATGTGATAATTAATCATTGTATTCCATCCACTTGATGCACTTGAAGATTTATCTCTATGTTCCTTTCTCTTTCGTTTTTTTTTCGAGGGGGGGTAGTTAATGTAAAGGTTGTGTTTCATCTATCACCTTGTCTTTCAATTGTACATTAAACTTCTTGTTTTGAGGAGATTCTAAAATGGATTAACGATTTTGTGTTTCAGGCATTTAATATCTTGCGTTATGAGATTGGGCAGAGGTATAATTCTCATTATGATGCGTTTAATCCTTCTGAATATGGACCACAGAAGAGCCAAAGGGTACAAAGTAGACACATCAGCTTTTCCTTTGTTCATTGCATGTACACACTCATAGGTTTATAAAATCATTAAGGGTTAGATGCAAGCATAAGCTAGGGCATGTCACTTCCAGTGATTGTTATGCAACTTGATGTCAATTCTAATAATGAAAGTGCTAAAAGTTGATTCTGTTAAATACATGTTATACTTGTAACTTGCTTTTTTATTGGTCATCTCCCTATGACTTGAATAGAGAGGATTTATCTGGATAAATTGAGCAGATACTTTCAGATTCAATCTTTTATGATGCATTTTTAAGAGTTCCATTGCATAAACACATCTTGATTCCCAACCCCACACCCCTCCACAACACCAAGAAAAACAAAATTCGTACTACCTTACCTAACTAACATTGTATCAACCACAAATTCAGGTGGCTTCCTTCTTGTTATACCTAACTGATGTTCAAGAAGGTGGAGAAACCATGTTTCCATTTGAGGTAAGACCATACGAACAAGCTGACCTTTATCTTGACATCTTTTACATCCAATCGTATGGAGCATTTACTTTTTTGATTGGCAGAATGGCTCGAATATGGATGGAAGCTATGATTACCAAAGATGTATTGGTTTGAAAGTGAAGCCACGCCAAGGTGATGGGCTTTTATTTTATTCTGTTTTCCCAAATGGTACAATCGATCTGGTTAGTGTTCCTGTTCCTATCCTCTGTTGCTTGAGTATGGTAGTTTTTATTTGAATACTGTATGAATTTTAATATTGCTGGACACTGAGTTAACGAGAGGGGGCATACACATACTTCGTTTGAGCCATGGAACTTATTGAATTCCATGTAGGAGCCTGATTCTGCCATGAATGGGCATGTTTGTTACAAAGAACAGTAGCCTATAGCAAAACTACGAACTAATATCAAGTTGAAGGTGAGGATTGTTAGGATCCCCATCCGAAGAAAATAACACTGAAATTGTAATACAATATATTATGAAAAGGAAAACACATAATGCTATAGCCTTTTGACAGGACCATCTCTGCCATAAGTCCCACAATGGACGAACTCCCTAATGATATGCCTAACCATTGAACCCCTTAATTTCCTCTATTTTTAGCCACCCTCCTAACAAACTATCAAGTAATTACAATTTTTCCACTAATATCCTAATAGCATTCTCAATATGTCCCCATTAATACTCTTACAAGGATCCTGACACTTTCTTTTGGTTGTTACTATTTTGGACATATTTGCTCACTGACCTAGATACACTTTGCCATGGACAAGGAACGTCAGACATTATGAATTATATGGACCCTTATTTTATGCAGACTGTAGGTGGACATAGACATGCTTCAGACGTATTTTGAGTGTGTAAATTTCGGATCATATGTATATATAAAACTATGTTAGTTTATTGCATTTAGACATATTCTATACAATAACCTACAACATTTAGATAGCTATCAAGTTTAAACCCACCATCTCTGGATCTCTGACTTCGTTAACTACTCTAGTGCATCATCCTTAGATAAATATAGTTTATTTTACAATATCTATTTTTGCTTTAAGATTGAGTTAATGGAAAAACATGACTTTAAATCTTTACATAATACATTTCATTACATTTGAAAGCATGAACGTCTGTAAAGTTAACAACAATTATCTTCAAAATCATATCTTGTGGTGTACTGTTTCTGTATTCTAATTTTCTTGAAAAAGGTTTATTTTTCATTTTTGTATTGGCCTTGCTTGTGCTATTCTGGTTGAAGTTTTCCACTAAAGAATGATGTTTGTCTGAGAATCTCATTTATTGGATGCTTTTGTAGACATCACTTCATGGAAGCTGTCCTGTGATTGA

mRNA sequence

ATGAAAGGGAAAATCGGAAAAGGTAATTGCAGCTTCAAATCGAAGCTAGGTTTGCCGGCGCTCCTCTTCGTTGTATGCCTATTTTGTTTCCTCGTTGGATTCTTCGGTTCTTCTCTCTTCTCTCAGGATGTAAATGGCGATAGGCCGAGGCCGAGGATGCTTCATTCGGTCAGCGATGGTAGCGATTACGATCCGATGCCATATGGAGAAGGCGGCGACGATTCCATTTCTTCGATTCCATTTCAGGTTTTGAGTTGGCAACCACGCGCAGTTTACTTTCCTAAGTTCGCAACTGCTGAGCAATGCCAAAACATAGTTAATACGGCGAAACCGAACCTTAAACCGTCTACCTTGGCTCTCCGTAAGGGAGAAACTGAAGAGAACACGAAGGGGATCCGGACAAGTTCTGGAGTTTTCTTGAGTGCTTCAGAAGATAAAAGTGGGACTCTGGATGACATCGAGAAAAAAATTGCAAGGGCAACTATGCTTCCAAGGACGCATGGAGAGGCATTTAATATCTTGCGTTATGAGATTGGGCAGAGGTATAATTCTCATTATGATGCGTTTAATCCTTCTGAATATGGACCACAGAAGAGCCAAAGGGTGGCTTCCTTCTTGTTATACCTAACTGATGTTCAAGAAGGTGGAGAAACCATGTTTCCATTTGAGAATGGCTCGAATATGGATGGAAGCTATGATTACCAAAGATGTATTGGTTTGAAAGTGAAGCCACGCCAAGACATCACTTCATGGAAGCTGTCCTGTGATTGA

Coding sequence (CDS)

ATGAAAGGGAAAATCGGAAAAGGTAATTGCAGCTTCAAATCGAAGCTAGGTTTGCCGGCGCTCCTCTTCGTTGTATGCCTATTTTGTTTCCTCGTTGGATTCTTCGGTTCTTCTCTCTTCTCTCAGGATGTAAATGGCGATAGGCCGAGGCCGAGGATGCTTCATTCGGTCAGCGATGGTAGCGATTACGATCCGATGCCATATGGAGAAGGCGGCGACGATTCCATTTCTTCGATTCCATTTCAGGTTTTGAGTTGGCAACCACGCGCAGTTTACTTTCCTAAGTTCGCAACTGCTGAGCAATGCCAAAACATAGTTAATACGGCGAAACCGAACCTTAAACCGTCTACCTTGGCTCTCCGTAAGGGAGAAACTGAAGAGAACACGAAGGGGATCCGGACAAGTTCTGGAGTTTTCTTGAGTGCTTCAGAAGATAAAAGTGGGACTCTGGATGACATCGAGAAAAAAATTGCAAGGGCAACTATGCTTCCAAGGACGCATGGAGAGGCATTTAATATCTTGCGTTATGAGATTGGGCAGAGGTATAATTCTCATTATGATGCGTTTAATCCTTCTGAATATGGACCACAGAAGAGCCAAAGGGTGGCTTCCTTCTTGTTATACCTAACTGATGTTCAAGAAGGTGGAGAAACCATGTTTCCATTTGAGAATGGCTCGAATATGGATGGAAGCTATGATTACCAAAGATGTATTGGTTTGAAAGTGAAGCCACGCCAAGACATCACTTCATGGAAGCTGTCCTGTGATTGA

Protein sequence

MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDGSDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGLKVKPRQDITSWKLSCD

Homology

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BLAST of Moc01g25940 vs. NCBI nr
Match: XP_022132603.1 (probable prolyl 4-hydroxylase 9 isoform X2 [Momordica charantia])

HSP 1 Score: 528.5 bits (1360), Expect = 3.2e-146
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG
Sbjct: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL
Sbjct: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL
Sbjct: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240

Query: 241 KVKPRQDITSWKLSCD 257
           KVKPRQDITSWKLSCD
Sbjct: 241 KVKPRQDITSWKLSCD 256

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BLAST of Moc01g25940 vs. NCBI nr
Match: XP_022132601.1 (probable prolyl 4-hydroxylase 9 isoform X1 [Momordica charantia])

HSP 1 Score: 505.8 bits (1301), Expect = 2.3e-139
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG
Sbjct: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL
Sbjct: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL
Sbjct: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

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BLAST of Moc01g25940 vs. NCBI nr
Match: XP_022977335.1 (probable prolyl 4-hydroxylase 9 [Cucurbita maxima])

HSP 1 Score: 422.9 bits (1086), Expect = 1.9e-114
Identity = 203/246 (82.52%), Postives = 224/246 (91.06%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGK GK + S +SKLGLPA++FV+CLFCFL GFFGSSL SQDV+ DRPRPR+L SV+ G
Sbjct: 1   MKGKSGKSSSSLRSKLGLPAVIFVLCLFCFLAGFFGSSLLSQDVDDDRPRPRLLQSVNGG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           S++D MP GE GDDSIS IPFQ+LSWQPRA+YFPKFATAEQC+NIVN A+P L+PS+LAL
Sbjct: 61  SEFDLMPSGENGDDSISLIPFQLLSWQPRALYFPKFATAEQCRNIVNMARPRLRPSSLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVF SASED+SGTL  IE+KIARATMLPR HGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFFSASEDESGTLGAIEEKIARATMLPRMHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           +YNSHYDAFNPSEYGPQKSQRVASFLLYLTDV+EGGETMFPFENG NMDG YDYQ+C GL
Sbjct: 181 KYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVEEGGETMFPFENGLNMDGKYDYQKCTGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

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BLAST of Moc01g25940 vs. NCBI nr
Match: XP_038881540.1 (probable prolyl 4-hydroxylase 9 [Benincasa hispida])

HSP 1 Score: 422.2 bits (1084), Expect = 3.3e-114
Identity = 205/246 (83.33%), Postives = 222/246 (90.24%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGK GK N S +SKLGLPAL+FV+CLFCFL GFFGSSL SQDV  DRP PR+L SVSDG
Sbjct: 1   MKGKSGKSNWSLRSKLGLPALIFVLCLFCFLAGFFGSSLLSQDVEDDRPGPRLLQSVSDG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           S++D M  GE GDDSISSIPFQVLSW+PRA++FPKFATAEQC +IVN A+P L+PSTLAL
Sbjct: 61  SEFDLMTPGENGDDSISSIPFQVLSWRPRALFFPKFATAEQCLSIVNMARPKLRPSTLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSG F  ASED+SGTL  IE+KIARATM+PRTHGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGAFFGASEDESGTLGVIEEKIARATMIPRTHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           +YNSHYDAFNPSEYGPQKSQRVASFLLYLTDV EGGETMFPFENGSNMDG YD+QRCIGL
Sbjct: 181 KYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVDEGGETMFPFENGSNMDGLYDFQRCIGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

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BLAST of Moc01g25940 vs. NCBI nr
Match: XP_023543701.1 (probable prolyl 4-hydroxylase 9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.8 bits (1083), Expect = 4.3e-114
Identity = 203/246 (82.52%), Postives = 223/246 (90.65%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MK K GK + S +SKLGLPA++FV+CLFCFL GFFGSSL SQDV+ DRPRPR+L SV+ G
Sbjct: 1   MKAKSGKSSSSLRSKLGLPAVIFVLCLFCFLAGFFGSSLLSQDVDDDRPRPRLLQSVNGG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           S++D MP GE GDDSIS IPFQVLSWQPRA+YFPKFATAEQC+NIVN A+P L+PS+LAL
Sbjct: 61  SEFDLMPSGENGDDSISLIPFQVLSWQPRALYFPKFATAEQCRNIVNMARPRLRPSSLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVF SASED+SGTL  IE+KIARATMLPR HGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFFSASEDESGTLGAIEEKIARATMLPRMHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           +YNSHYDAFNPSEYGPQKSQRVASFLLYLTDV+EGGETMFPFENG NMDG YDYQ+C GL
Sbjct: 181 KYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVEEGGETMFPFENGLNMDGKYDYQKCTGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

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BLAST of Moc01g25940 vs. ExPASy Swiss-Prot
Match: Q8VZJ7 (Probable prolyl 4-hydroxylase 9 OS=Arabidopsis thaliana OX=3702 GN=P4H9 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.0e-90
Identity = 162/235 (68.94%), Postives = 194/235 (82.55%), Query Frame = 0


Query: 13  KSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG-SDYDPMPYGEG 72
           + KLGL A + V C  CFL GF+GS+L SQ+V   +PR RML  V +G  +   MP+G  
Sbjct: 10  RKKLGL-ATVIVFCSLCFLFGFYGSTLLSQNVPRVKPRLRMLDMVENGEEEASSMPHGVT 69

Query: 73  GDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKG 132
           G++SI SIPFQVLSW+PRA+YFP FATAEQCQ I+  AK NLKPS LALRKGET ENTKG
Sbjct: 70  GEESIGSIPFQVLSWRPRAIYFPNFATAEQCQAIIERAKVNLKPSALALRKGETAENTKG 129

Query: 133 IRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNP 192
            RTSSG F+SASE+ +G LD +E+KIARATM+PR+HGE+FNILRYE+GQ+Y+SHYD FNP
Sbjct: 130 TRTSSGTFISASEESTGALDFVERKIARATMIPRSHGESFNILRYELGQKYDSHYDVFNP 189

Query: 193 SEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGLKVKPRQ 247
           +EYGPQ SQR+ASFLLYL+DV+EGGETMFPFENGSNM   YDY++CIGLKVKPR+
Sbjct: 190 TEYGPQSSQRIASFLLYLSDVEEGGETMFPFENGSNMGIGYDYKQCIGLKVKPRK 243

[Close]

BLAST of Moc01g25940 vs. ExPASy Swiss-Prot
Match: F4ILF8 (Prolyl 4-hydroxylase 13 OS=Arabidopsis thaliana OX=3702 GN=P4H13 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 5.2e-62
Identity = 122/234 (52.14%), Postives = 161/234 (68.80%), Query Frame = 0


Query: 13  KSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDGSDYDPMPYGEGG 72
           + KL  P +    C F  + GF   +LFSQ ++     P    SV+D  + D + +G   
Sbjct: 7   EKKLVFPYVFIACCFFLAIFGFCFFNLFSQGISFSE-IPTTRRSVND--ETDSLDHG--- 66

Query: 73  DDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKGI 132
             S+S+IPF  LSW PR  Y P FAT +QC+ +++ AKP LKPSTLALRKGET E T+  
Sbjct: 67  -SSVSNIPFHGLSWNPRVFYLPNFATKQQCEAVIDMAKPKLKPSTLALRKGETAETTQNY 126

Query: 133 RTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNPS 192
           R+   +     ED+SG L  IE+KIA AT  P+ + E+FNILRY++GQ+Y+SHYDAF+ +
Sbjct: 127 RS---LHQHTDEDESGVLAAIEEKIALATRFPKDYYESFNILRYQLGQKYDSHYDAFHSA 186

Query: 193 EYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGLKVKPRQ 247
           EYGP  SQRV +FLL+L+ V+EGGETMFPFENG NM+G YDY++C+GLKVKPRQ
Sbjct: 187 EYGPLISQRVVTFLLFLSSVEEGGETMFPFENGRNMNGRYDYEKCVGLKVKPRQ 230

[Close]

BLAST of Moc01g25940 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.0e-30
Identity = 74/192 (38.54%), Postives = 112/192 (58.33%), Query Frame = 0


Query: 71  GGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTK 130
           G DDS +    +++SW+PRA  +  F T E+C+ ++  AKP+++ ST+   K     +++
Sbjct: 68  GEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSR 127

Query: 131 GIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFN 190
            +RTSSG FL+   DK  T+ +IEK+I+  T +P  HGE   +L YEIGQ+Y  HYD F 
Sbjct: 128 -VRTSSGTFLARGRDK--TIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFM 187

Query: 191 PSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENG--SNMDGSYDYQRC--IGLKVKPRQ 250
                    QR+A+ L+YL+DV+EGGET+FP   G  S +    +   C   GL VKP+ 
Sbjct: 188 DEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKM 247

Query: 251 D--ITSWKLSCD 257
              +  W ++ D
Sbjct: 248 GDALLFWSMTPD 256

[Close]

BLAST of Moc01g25940 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-28
Identity = 81/229 (35.37%), Postives = 123/229 (53.71%), Query Frame = 0


Query: 21  LLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDGSDYDPMPYGEGGDDSISSIP 80
           +LF++ +   ++  FG  +FS  +N D   P  L      +       G+ GD       
Sbjct: 23  MLFMLTIVLLMLLAFG--VFSLPINNDESSPIDLSYFRRAATERSEGLGKRGDQWT---- 82

Query: 81  FQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKGIRTSSGVFL 140
            +VLSW+PRA  +  F + E+C+ +++ AKP++  ST+   +    ++++ +RTSSG FL
Sbjct: 83  -EVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSR-VRTSSGTFL 142

Query: 141 SASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNPSEYGPQKSQ 200
               DK   +  IEK+IA  T +P  HGE   +L YE GQ+Y  HYD F          Q
Sbjct: 143 RRGRDK--IIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQ 202

Query: 201 RVASFLLYLTDVQEGGETMFPFE--NGSNMDGSYDYQRC--IGLKVKPR 246
           R+A+ L+YL+DV+EGGET+FP    N S++    +   C   GL VKPR
Sbjct: 203 RMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPR 241

[Close]

BLAST of Moc01g25940 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.6e-26
Identity = 65/182 (35.71%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 82  QVLSWQPRAVYFPKFATAEQCQNIVNTAKPN-LKPSTLALRKGETEENTKGIRTSSGVFL 141
           +V+SW+PRA  +  F T E+C+++++ AKP+ +K   + ++ G++ ++   +RTSSG FL
Sbjct: 81  EVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTGKSIDSR--VRTSSGTFL 140

Query: 142 SASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNPSEYGPQKSQ 201
           +   D+   +++IE +I+  T +P  +GE   +L YE+GQRY  H+D F       +  Q
Sbjct: 141 NRGHDE--IVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHDYFFDEFNVRKGGQ 200

Query: 202 RVASFLLYLTDVQEGGETMFPFENGSNMDGSY--DYQRC--IGLKVKP--RQDITSWKLS 257
           R+A+ L+YL+DV EGGET+FP   G+  D  +  +  +C   GL V P  R  +  W + 
Sbjct: 201 RIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSVLPKKRDALLFWSMK 258

[Close]

BLAST of Moc01g25940 vs. ExPASy TrEMBL
Match: A0A6J1BWQ9 (probable prolyl 4-hydroxylase 9 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005426 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.6e-146
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG
Sbjct: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL
Sbjct: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL
Sbjct: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240

Query: 241 KVKPRQDITSWKLSCD 257
           KVKPRQDITSWKLSCD
Sbjct: 241 KVKPRQDITSWKLSCD 256

[Close]

BLAST of Moc01g25940 vs. ExPASy TrEMBL
Match: A0A6J1BSQ4 (probable prolyl 4-hydroxylase 9 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005426 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.1e-139
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG
Sbjct: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL
Sbjct: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL
Sbjct: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

[Close]

BLAST of Moc01g25940 vs. ExPASy TrEMBL
Match: A0A6J1IM09 (probable prolyl 4-hydroxylase 9 OS=Cucurbita maxima OX=3661 GN=LOC111477685 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 9.3e-115
Identity = 203/246 (82.52%), Postives = 224/246 (91.06%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MKGK GK + S +SKLGLPA++FV+CLFCFL GFFGSSL SQDV+ DRPRPR+L SV+ G
Sbjct: 1   MKGKSGKSSSSLRSKLGLPAVIFVLCLFCFLAGFFGSSLLSQDVDDDRPRPRLLQSVNGG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           S++D MP GE GDDSIS IPFQ+LSWQPRA+YFPKFATAEQC+NIVN A+P L+PS+LAL
Sbjct: 61  SEFDLMPSGENGDDSISLIPFQLLSWQPRALYFPKFATAEQCRNIVNMARPRLRPSSLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVF SASED+SGTL  IE+KIARATMLPR HGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFFSASEDESGTLGAIEEKIARATMLPRMHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           +YNSHYDAFNPSEYGPQKSQRVASFLLYLTDV+EGGETMFPFENG NMDG YDYQ+C GL
Sbjct: 181 KYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVEEGGETMFPFENGLNMDGKYDYQKCTGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

[Close]

BLAST of Moc01g25940 vs. ExPASy TrEMBL
Match: A0A6J1GEZ7 (probable prolyl 4-hydroxylase 9 OS=Cucurbita moschata OX=3662 GN=LOC111453589 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.8e-113
Identity = 202/246 (82.11%), Postives = 221/246 (89.84%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MK K GK   S +SKLGLPA++FV+CLFCFL GFFGSSL SQDV+ DRPRPR+L SV+ G
Sbjct: 1   MKAKSGKSCSSLRSKLGLPAVIFVLCLFCFLAGFFGSSLLSQDVDDDRPRPRLLQSVNGG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           SD+D MP GE GDDSIS IPFQVLSWQPRA+YFPKFATAEQC+NIVN A+P L+PS+LAL
Sbjct: 61  SDFDLMPSGENGDDSISLIPFQVLSWQPRALYFPKFATAEQCRNIVNMARPRLRPSSLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGETEENTKGIRTSSGVF SASED+SGTL  IE+KIAR TMLPR HGEAFNILRYEIGQ
Sbjct: 121 RKGETEENTKGIRTSSGVFFSASEDESGTLGAIEEKIARVTMLPRMHGEAFNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           +YNSHYDAFNPSEYGPQKSQRVASFLLYLTDV++GGETMFPFENG NMDG YDYQ+C GL
Sbjct: 181 KYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVEDGGETMFPFENGLNMDGRYDYQKCTGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

[Close]

BLAST of Moc01g25940 vs. ExPASy TrEMBL
Match: A0A5D3CP58 (Putative prolyl 4-hydroxylase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004260 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.7e-113
Identity = 201/246 (81.71%), Postives = 222/246 (90.24%), Query Frame = 0


Query: 1   MKGKIGKGNCSFKSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG 60
           MK K GK N S +SKLGLPAL+FV+CLFCFL GFFGSSL SQDV+ DRPR R+L S SDG
Sbjct: 1   MKAKSGKSNWSLRSKLGLPALIFVLCLFCFLAGFFGSSLLSQDVDDDRPRSRLLQSASDG 60

Query: 61  SDYDPMPYGEGGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLAL 120
           +++D M  GE GDDSISSIPFQVLSW+PRA+YFPKFATAEQCQ+IVN AKP L+PSTLAL
Sbjct: 61  TEFDLMSSGENGDDSISSIPFQVLSWRPRALYFPKFATAEQCQSIVNMAKPKLRPSTLAL 120

Query: 121 RKGETEENTKGIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQ 180
           RKGET ENTKGIRTSSGVF SASED+SG L  IE+KIARATM+PRTHGEA+NILRYEIGQ
Sbjct: 121 RKGETAENTKGIRTSSGVFFSASEDESGILGVIEEKIARATMIPRTHGEAYNILRYEIGQ 180

Query: 181 RYNSHYDAFNPSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGL 240
           +YNSHYDAF PSEYGPQKSQRVASFLLYLTDV+EGGETMFPFENGSNMDG+Y+YQ C+GL
Sbjct: 181 KYNSHYDAFKPSEYGPQKSQRVASFLLYLTDVEEGGETMFPFENGSNMDGTYNYQACVGL 240

Query: 241 KVKPRQ 247
           KVKPRQ
Sbjct: 241 KVKPRQ 246

[Close]

BLAST of Moc01g25940 vs. TAIR 10
Match: AT4G33910.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.4e-91
Identity = 162/235 (68.94%), Postives = 194/235 (82.55%), Query Frame = 0


Query: 13  KSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDG-SDYDPMPYGEG 72
           + KLGL A + V C  CFL GF+GS+L SQ+V   +PR RML  V +G  +   MP+G  
Sbjct: 10  RKKLGL-ATVIVFCSLCFLFGFYGSTLLSQNVPRVKPRLRMLDMVENGEEEASSMPHGVT 69

Query: 73  GDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKG 132
           G++SI SIPFQVLSW+PRA+YFP FATAEQCQ I+  AK NLKPS LALRKGET ENTKG
Sbjct: 70  GEESIGSIPFQVLSWRPRAIYFPNFATAEQCQAIIERAKVNLKPSALALRKGETAENTKG 129

Query: 133 IRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNP 192
            RTSSG F+SASE+ +G LD +E+KIARATM+PR+HGE+FNILRYE+GQ+Y+SHYD FNP
Sbjct: 130 TRTSSGTFISASEESTGALDFVERKIARATMIPRSHGESFNILRYELGQKYDSHYDVFNP 189

Query: 193 SEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGLKVKPRQ 247
           +EYGPQ SQR+ASFLLYL+DV+EGGETMFPFENGSNM   YDY++CIGLKVKPR+
Sbjct: 190 TEYGPQSSQRIASFLLYLSDVEEGGETMFPFENGSNMGIGYDYKQCIGLKVKPRK 243

[Close]

BLAST of Moc01g25940 vs. TAIR 10
Match: AT2G23096.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 3.7e-63
Identity = 122/234 (52.14%), Postives = 161/234 (68.80%), Query Frame = 0


Query: 13  KSKLGLPALLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDGSDYDPMPYGEGG 72
           + KL  P +    C F  + GF   +LFSQ ++     P    SV+D  + D + +G   
Sbjct: 7   EKKLVFPYVFIACCFFLAIFGFCFFNLFSQGISFSE-IPTTRRSVND--ETDSLDHG--- 66

Query: 73  DDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKGI 132
             S+S+IPF  LSW PR  Y P FAT +QC+ +++ AKP LKPSTLALRKGET E T+  
Sbjct: 67  -SSVSNIPFHGLSWNPRVFYLPNFATKQQCEAVIDMAKPKLKPSTLALRKGETAETTQNY 126

Query: 133 RTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNPS 192
           R+   +     ED+SG L  IE+KIA AT  P+ + E+FNILRY++GQ+Y+SHYDAF+ +
Sbjct: 127 RS---LHQHTDEDESGVLAAIEEKIALATRFPKDYYESFNILRYQLGQKYDSHYDAFHSA 186

Query: 193 EYGPQKSQRVASFLLYLTDVQEGGETMFPFENGSNMDGSYDYQRCIGLKVKPRQ 247
           EYGP  SQRV +FLL+L+ V+EGGETMFPFENG NM+G YDY++C+GLKVKPRQ
Sbjct: 187 EYGPLISQRVVTFLLFLSSVEEGGETMFPFENGRNMNGRYDYEKCVGLKVKPRQ 230

[Close]

BLAST of Moc01g25940 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 133.3 bits (334), Expect = 2.8e-31
Identity = 74/192 (38.54%), Postives = 112/192 (58.33%), Query Frame = 0


Query: 71  GGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTK 130
           G DDS +    +++SW+PRA  +  F T E+C+ ++  AKP+++ ST+   K     +++
Sbjct: 68  GEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSR 127

Query: 131 GIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFN 190
            +RTSSG FL+   DK  T+ +IEK+I+  T +P  HGE   +L YEIGQ+Y  HYD F 
Sbjct: 128 -VRTSSGTFLARGRDK--TIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFM 187

Query: 191 PSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENG--SNMDGSYDYQRC--IGLKVKPRQ 250
                    QR+A+ L+YL+DV+EGGET+FP   G  S +    +   C   GL VKP+ 
Sbjct: 188 DEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKM 247

Query: 251 D--ITSWKLSCD 257
              +  W ++ D
Sbjct: 248 GDALLFWSMTPD 256

[Close]

BLAST of Moc01g25940 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 9.1e-30
Identity = 81/229 (35.37%), Postives = 123/229 (53.71%), Query Frame = 0


Query: 21  LLFVVCLFCFLVGFFGSSLFSQDVNGDRPRPRMLHSVSDGSDYDPMPYGEGGDDSISSIP 80
           +LF++ +   ++  FG  +FS  +N D   P  L      +       G+ GD       
Sbjct: 23  MLFMLTIVLLMLLAFG--VFSLPINNDESSPIDLSYFRRAATERSEGLGKRGDQWT---- 82

Query: 81  FQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTKGIRTSSGVFL 140
            +VLSW+PRA  +  F + E+C+ +++ AKP++  ST+   +    ++++ +RTSSG FL
Sbjct: 83  -EVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSR-VRTSSGTFL 142

Query: 141 SASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFNPSEYGPQKSQ 200
               DK   +  IEK+IA  T +P  HGE   +L YE GQ+Y  HYD F          Q
Sbjct: 143 RRGRDK--IIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQ 202

Query: 201 RVASFLLYLTDVQEGGETMFPFE--NGSNMDGSYDYQRC--IGLKVKPR 246
           R+A+ L+YL+DV+EGGET+FP    N S++    +   C   GL VKPR
Sbjct: 203 RMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPR 241

[Close]

BLAST of Moc01g25940 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 2.2e-28
Identity = 64/156 (41.03%), Postives = 97/156 (62.18%), Query Frame = 0


Query: 71  GGDDSISSIPFQVLSWQPRAVYFPKFATAEQCQNIVNTAKPNLKPSTLALRKGETEENTK 130
           G DDS +    +++SW+PRA  +  F   E+C+ ++  AKP+++ ST+   K     +++
Sbjct: 68  GEDDSKNERWVEIISWEPRASVYHNF--LEECKYLIELAKPHMEKSTVVDEKTGKSTDSR 127

Query: 131 GIRTSSGVFLSASEDKSGTLDDIEKKIARATMLPRTHGEAFNILRYEIGQRYNSHYDAFN 190
            +RTSSG FL+   DK  T+ +IEK+I+  T +P  HGE   +L YEIGQ+Y  HYD F 
Sbjct: 128 -VRTSSGTFLARGRDK--TIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFM 187

Query: 191 PSEYGPQKSQRVASFLLYLTDVQEGGETMFPFENGS 227
                    QR+A+ L+YL+DV+EGGET+FP   G+
Sbjct: 188 DEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGN 218

The following BLAST results are available for this feature:

BLAST of Moc01g25940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132603.1	3.2e-146	100.00	probable prolyl 4-hydroxylase 9 isoform X2 [Momordica charantia]	[more]
XP_022132601.1	2.3e-139	100.00	probable prolyl 4-hydroxylase 9 isoform X1 [Momordica charantia]	[more]
XP_022977335.1	1.9e-114	82.52	probable prolyl 4-hydroxylase 9 [Cucurbita maxima]	[more]
XP_038881540.1	3.3e-114	83.33	probable prolyl 4-hydroxylase 9 [Benincasa hispida]	[more]
XP_023543701.1	4.3e-114	82.52	probable prolyl 4-hydroxylase 9 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc01g25940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZJ7	2.0e-90	68.94	Probable prolyl 4-hydroxylase 9 OS=Arabidopsis thaliana OX=3702 GN=P4H9 PE=2 SV=...	[more]
F4ILF8	5.2e-62	52.14	Prolyl 4-hydroxylase 13 OS=Arabidopsis thaliana OX=3702 GN=P4H13 PE=2 SV=1	[more]
F4JZ24	4.0e-30	38.54	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q9LN20	1.3e-28	35.37	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
F4JNU8	4.6e-26	35.71	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]

BLAST of Moc01g25940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWQ9	1.6e-146	100.00	probable prolyl 4-hydroxylase 9 isoform X2 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1BSQ4	1.1e-139	100.00	probable prolyl 4-hydroxylase 9 isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1IM09	9.3e-115	82.52	probable prolyl 4-hydroxylase 9 OS=Cucurbita maxima OX=3661 GN=LOC111477685 PE=4...	[more]
A0A6J1GEZ7	1.8e-113	82.11	probable prolyl 4-hydroxylase 9 OS=Cucurbita moschata OX=3662 GN=LOC111453589 PE...	[more]
A0A5D3CP58	6.7e-113	81.71	Putative prolyl 4-hydroxylase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of Moc01g25940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33910.1	1.4e-91	68.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G23096.1	3.7e-63	52.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.1	2.8e-31	38.54	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	9.1e-30	35.37	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	2.2e-28	41.03	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 88..253 e-value: 1.4E-18 score: 77.7
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 172..220 e-value: 1.8E-7 score: 31.9
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 81..248 e-value: 1.6E-42 score: 147.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..69
None	No IPR available	PANTHER	PTHR10869:SF189	TYPE 1 PROLY 4-HYDROXYLASE	coord: 8..246
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 8..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g25940.1	Moc01g25940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
biological_process	GO:0080147	root hair cell development
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen