Moc01g25890 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc01g25890 vs. NCBI nr
Match: XP_038882249.1 (uncharacterized protein LOC120073475 [Benincasa hispida])

HSP 1 Score: 408.3 bits (1048), Expect = 6.8e-110
Identity = 246/366 (67.21%), Postives = 270/366 (73.77%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVET---AADEEFDFG 69
           E W E G YM +PD EDESLSLCDLP+NL+K+DNP+ L E KPRA ET   AAD+EFDFG
Sbjct: 8   ERWREDGFYMAEPDEEDESLSLCDLPINLVKDDNPHFLSEIKPRAGETPVAAADDEFDFG 67

Query: 70  SLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRS 129
            L  FSNSAAQP PEMCLADEV YQGQLLPVRLSVSSDN+SA  Y RHGSGSGS   FRS
Sbjct: 68  FLRDFSNSAAQPLPEMCLADEVFYQGQLLPVRLSVSSDNTSARFYHRHGSGSGSFANFRS 127

Query: 130 VNSSGSSSSRRSQCSSS-----SITSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFRQ 189
           +NSS SSSS RSQCSSS     S+T+TSAAR SR  V NLFHSHPSPKPQIKA+PTTFRQ
Sbjct: 128 INSSRSSSS-RSQCSSSSATNNSLTTTSAARISRLTVPNLFHSHPSPKPQIKASPTTFRQ 187

Query: 190 ASTGGRSQKSST-WDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPT---S 249
           A+TGG  QKSS+ WDFFRLGV K PGMELQDLKHRSSTRR          K+FIP    +
Sbjct: 188 ANTGGGRQKSSSPWDFFRLGVVKTPGMELQDLKHRSSTRRNSSVSRSNSGKAFIPAAAIA 247

Query: 250 GGGGSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSS 309
               SS KE T +PRA + IE  +  GGILSGVGCKCSV+STVAP P KAVVV RR  + 
Sbjct: 248 ASNESSVKEMTRKPRASVSIE--RKGGGILSGVGCKCSVDSTVAPAP-KAVVVRRRKVNG 307

Query: 310 NKITAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSH 354
             I AQ              AMRELR+KKVEKEKE++EKLALSR RTFEWLKE    LSH
Sbjct: 308 KNIPAQ--------------AMRELRIKKVEKEKEEEEKLALSRQRTFEWLKE----LSH 351

BLAST of Moc01g25890 vs. NCBI nr
Match: XP_011658482.1 (uncharacterized protein LOC105436026 [Cucumis sativus])

HSP 1 Score: 386.3 bits (991), Expect = 2.8e-103
Identity = 233/363 (64.19%), Postives = 259/363 (71.35%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVET---AADEEFDFG 69
           E   E  +YME+PD  DESLSLCDLP+NL+K+ NP+ L E KPRA ET   AAD+EFDFG
Sbjct: 8   ERCGEDKLYMEEPDENDESLSLCDLPINLVKDGNPHFLLEIKPRAGETSIAAADDEFDFG 67

Query: 70  SLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRS 129
           SL GFSN A QPPPEMCLADEV YQGQLLPVRLSVSSDN+SAG+YRRH          RS
Sbjct: 68  SLGGFSNLADQPPPEMCLADEVFYQGQLLPVRLSVSSDNTSAGVYRRH---------LRS 127

Query: 130 VNSSGSSSSRRSQCSSSSITS-----TSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFRQ 189
           +NSSGSSSS RSQCSSSS+T+     TSAARN + RV NLFHSHPSPKPQIKA+P TFRQ
Sbjct: 128 INSSGSSSS-RSQCSSSSVTNNSLTITSAARNPQLRVPNLFHSHPSPKPQIKASPMTFRQ 187

Query: 190 ASTGGRSQKSS-TWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGGG 249
           ASTGG  QKSS  WDFFRLGV K PGMEL+DLKHRSSTRR          K+F+P     
Sbjct: 188 ASTGGNRQKSSFGWDFFRLGVVKTPGMELEDLKHRSSTRRNSSVSRSNSEKAFVPAPLSN 247

Query: 250 GSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNKI 309
             S K    +PR  +LIE  + S GIL GVGCKCSVESTVAP P KA+V VRR G+S  I
Sbjct: 248 ERSVKVMMRKPRESVLIE--RKSRGILGGVGCKCSVESTVAPAP-KAMVGVRRKGNSKNI 307

Query: 310 TAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHATL 354
            AQA             A RE+RMKKV KEKE++EKL LSRHRTFEWLKE    LSHA  
Sbjct: 308 PAQAT------------AKREMRMKKVGKEKEEEEKLELSRHRTFEWLKE----LSHANF 341

BLAST of Moc01g25890 vs. NCBI nr
Match: KGN48582.1 (hypothetical protein Csa_004362 [Cucumis sativus])

HSP 1 Score: 386.3 bits (991), Expect = 2.8e-103
Identity = 233/363 (64.19%), Postives = 259/363 (71.35%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVET---AADEEFDFG 69
           E   E  +YME+PD  DESLSLCDLP+NL+K+ NP+ L E KPRA ET   AAD+EFDFG
Sbjct: 10  ERCGEDKLYMEEPDENDESLSLCDLPINLVKDGNPHFLLEIKPRAGETSIAAADDEFDFG 69

Query: 70  SLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRS 129
           SL GFSN A QPPPEMCLADEV YQGQLLPVRLSVSSDN+SAG+YRRH          RS
Sbjct: 70  SLGGFSNLADQPPPEMCLADEVFYQGQLLPVRLSVSSDNTSAGVYRRH---------LRS 129

Query: 130 VNSSGSSSSRRSQCSSSSITS-----TSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFRQ 189
           +NSSGSSSS RSQCSSSS+T+     TSAARN + RV NLFHSHPSPKPQIKA+P TFRQ
Sbjct: 130 INSSGSSSS-RSQCSSSSVTNNSLTITSAARNPQLRVPNLFHSHPSPKPQIKASPMTFRQ 189

Query: 190 ASTGGRSQKSS-TWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGGG 249
           ASTGG  QKSS  WDFFRLGV K PGMEL+DLKHRSSTRR          K+F+P     
Sbjct: 190 ASTGGNRQKSSFGWDFFRLGVVKTPGMELEDLKHRSSTRRNSSVSRSNSEKAFVPAPLSN 249

Query: 250 GSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNKI 309
             S K    +PR  +LIE  + S GIL GVGCKCSVESTVAP P KA+V VRR G+S  I
Sbjct: 250 ERSVKVMMRKPRESVLIE--RKSRGILGGVGCKCSVESTVAPAP-KAMVGVRRKGNSKNI 309

Query: 310 TAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHATL 354
            AQA             A RE+RMKKV KEKE++EKL LSRHRTFEWLKE    LSHA  
Sbjct: 310 PAQAT------------AKREMRMKKVGKEKEEEEKLELSRHRTFEWLKE----LSHANF 343

BLAST of Moc01g25890 vs. NCBI nr
Match: KAG7034310.1 (hypothetical protein SDJN02_04037, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 385.2 bits (988), Expect = 6.2e-103
Identity = 231/364 (63.46%), Postives = 257/364 (70.60%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRA----VETAADEEFDF 69
           E W E   YMEQ DHEDE+LSLCDLP++++K+D P+ LFE+KP A    +  A D+EFDF
Sbjct: 8   EGWGEDEFYMEQLDHEDETLSLCDLPIHVVKDDKPHFLFEEKPGAGENPIAAADDDEFDF 67

Query: 70  GSLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFR 129
           GSL GFSNS+AQPPP+MCLADEV YQG+LLPVRLSVSSD +SAG +RR GSGSGS G  R
Sbjct: 68  GSLGGFSNSSAQPPPQMCLADEVFYQGKLLPVRLSVSSDTTSAGFHRRQGSGSGSFGNIR 127

Query: 130 SVNSSGSSSSRRSQCSSS-----SITSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFR 189
           S+NSSGSSSS RSQCSSS     SITSTSA RNS PRV NLFHSHPSPKPQIKA+P TFR
Sbjct: 128 SINSSGSSSS-RSQCSSSSVTTTSITSTSAVRNSLPRVPNLFHSHPSPKPQIKASPITFR 187

Query: 190 QAST-GGRSQKSSTWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGG 249
           QAST GGR   SS WDFFRLGV KAPGMELQDLKHRSSTRR          K+FIP +  
Sbjct: 188 QASTGGGRQISSSKWDFFRLGVVKAPGMELQDLKHRSSTRRNSSVSRSDSEKAFIP-AAS 247

Query: 250 GGSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNK 309
            G+S KE   RPRA I IE ++  GGILSGVGCKCSV STV P P K VVV RR G+   
Sbjct: 248 NGNSVKEIMRRPRASISIERKR--GGILSGVGCKCSVGSTVTPAP-KTVVVRRRKGN--- 307

Query: 310 ITAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHAT 354
                                  R    EKEKE+QEKL LSR RTFEWLKE    LSHA+
Sbjct: 308 -----------------------RKNTPEKEKEEQEKLTLSRDRTFEWLKE----LSHAS 336

BLAST of Moc01g25890 vs. NCBI nr
Match: KAG6604149.1 (hypothetical protein SDJN03_04758, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 381.3 bits (978), Expect = 8.9e-102
Identity = 229/364 (62.91%), Postives = 256/364 (70.33%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRA----VETAADEEFDF 69
           E W E   YMEQ DHEDE+LSLCDLP++++K+D P+ LFE+KP A    +  A D+EFDF
Sbjct: 8   EGWGEDEFYMEQLDHEDETLSLCDLPIHVVKDDKPHFLFEEKPGAGENPIAAADDDEFDF 67

Query: 70  GSLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFR 129
           GSL  FSNS+AQPPP+MCLADEV YQG+LLPVRLSVSSD +SAG +RR GSGSGS G  R
Sbjct: 68  GSLGSFSNSSAQPPPQMCLADEVFYQGKLLPVRLSVSSDTTSAGFHRRQGSGSGSFGNIR 127

Query: 130 SVNSSGSSSSRRSQCSSS-----SITSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFR 189
           S+NSSGSSSS RSQCSSS     SITSTSA RNS PRV NLFHSHPSPKPQIKA+P TFR
Sbjct: 128 SINSSGSSSS-RSQCSSSSVTTTSITSTSAVRNSLPRVPNLFHSHPSPKPQIKASPITFR 187

Query: 190 QAST-GGRSQKSSTWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGG 249
           QAST GGR   SS WDFFRLGV KAPGMELQDLKHRSSTRR          K+FIP +  
Sbjct: 188 QASTGGGRQISSSKWDFFRLGVVKAPGMELQDLKHRSSTRRNSSVSRSDSEKAFIP-AAS 247

Query: 250 GGSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNK 309
            G+S KE   RP A + IE ++  GGILSGVGCKCSV STV P P K VVV RR G+   
Sbjct: 248 NGNSVKEIMRRPPASVSIERKR--GGILSGVGCKCSVGSTVTPAP-KTVVVRRRKGN--- 307

Query: 310 ITAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHAT 354
                                  R    EKEKE+QEKLALSR RTFEWLKE    LSHA+
Sbjct: 308 -----------------------RKNTPEKEKEEQEKLALSRDRTFEWLKE----LSHAS 336

BLAST of Moc01g25890 vs. ExPASy TrEMBL
Match: A0A0A0KJN1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493900 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.3e-103
Identity = 233/363 (64.19%), Postives = 259/363 (71.35%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVET---AADEEFDFG 69
           E   E  +YME+PD  DESLSLCDLP+NL+K+ NP+ L E KPRA ET   AAD+EFDFG
Sbjct: 10  ERCGEDKLYMEEPDENDESLSLCDLPINLVKDGNPHFLLEIKPRAGETSIAAADDEFDFG 69

Query: 70  SLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRS 129
           SL GFSN A QPPPEMCLADEV YQGQLLPVRLSVSSDN+SAG+YRRH          RS
Sbjct: 70  SLGGFSNLADQPPPEMCLADEVFYQGQLLPVRLSVSSDNTSAGVYRRH---------LRS 129

Query: 130 VNSSGSSSSRRSQCSSSSITS-----TSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFRQ 189
           +NSSGSSSS RSQCSSSS+T+     TSAARN + RV NLFHSHPSPKPQIKA+P TFRQ
Sbjct: 130 INSSGSSSS-RSQCSSSSVTNNSLTITSAARNPQLRVPNLFHSHPSPKPQIKASPMTFRQ 189

Query: 190 ASTGGRSQKSS-TWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGGG 249
           ASTGG  QKSS  WDFFRLGV K PGMEL+DLKHRSSTRR          K+F+P     
Sbjct: 190 ASTGGNRQKSSFGWDFFRLGVVKTPGMELEDLKHRSSTRRNSSVSRSNSEKAFVPAPLSN 249

Query: 250 GSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNKI 309
             S K    +PR  +LIE  + S GIL GVGCKCSVESTVAP P KA+V VRR G+S  I
Sbjct: 250 ERSVKVMMRKPRESVLIE--RKSRGILGGVGCKCSVESTVAPAP-KAMVGVRRKGNSKNI 309

Query: 310 TAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHATL 354
            AQA             A RE+RMKKV KEKE++EKL LSRHRTFEWLKE    LSHA  
Sbjct: 310 PAQAT------------AKREMRMKKVGKEKEEEEKLELSRHRTFEWLKE----LSHANF 343

BLAST of Moc01g25890 vs. ExPASy TrEMBL
Match: A0A5D3CNV0 (Putative membrane-associated kinase regulator 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004300 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 2.1e-101
Identity = 229/359 (63.79%), Postives = 260/359 (72.42%), Query Frame = 0

Query: 14  EGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVE---TAADEEFDFGSLWG 73
           E  +YME+PD +DESLSLCDLP+NL+K+DNP+ L E KPRA +    AAD++FDFGSL G
Sbjct: 13  EDELYMEEPDEKDESLSLCDLPINLVKDDNPHFLLEIKPRAGKASIAAADDDFDFGSLGG 72

Query: 74  FSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRSVNSS 133
           FSN A QPPPEMCLADEV YQGQLLP+RLSVSSDN+SAG+YRRH          RS+NSS
Sbjct: 73  FSNLADQPPPEMCLADEVFYQGQLLPLRLSVSSDNTSAGVYRRH---------LRSINSS 132

Query: 134 GSSSSRRSQCSSSSI-----TSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFRQASTG 193
           GSSSS RSQCSSSS+     T+TSAAR+ R RV NLFHSHPSPKPQIKA+P T RQASTG
Sbjct: 133 GSSSS-RSQCSSSSVTNNSLTTTSAARDPRLRVPNLFHSHPSPKPQIKASPITSRQASTG 192

Query: 194 GRSQKSS-TWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGGGGSSD 253
           G  QKSS TWDFFRLGV K PGMEL+DLKHRSSTRR          K+F+P +     S 
Sbjct: 193 GNRQKSSFTWDFFRLGVVKTPGMELEDLKHRSSTRRNSSVSRSNSEKAFVPAAPSNERSV 252

Query: 254 KEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNKITAQA 313
           K    RPR  +LIE RKSS GILSGVGCKCSV STVAP P KA+VVVRR G+   I A++
Sbjct: 253 KVMMRRPRESVLIE-RKSS-GILSGVGCKCSVGSTVAPAP-KAMVVVRRKGNGKNIPAKS 312

Query: 314 ATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHATLPCD 354
                           ELRMKK+ KEKE++EKL LSRHRTFEWLKE    LSHA   C+
Sbjct: 313 ----------------ELRMKKLGKEKEEEEKLELSRHRTFEWLKE----LSHANFSCE 338

BLAST of Moc01g25890 vs. ExPASy TrEMBL
Match: A0A1S3B0H1 (probable membrane-associated kinase regulator 1 OS=Cucumis melo OX=3656 GN=LOC103484825 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 2.1e-101
Identity = 229/359 (63.79%), Postives = 260/359 (72.42%), Query Frame = 0

Query: 14  EGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVE---TAADEEFDFGSLWG 73
           E  +YME+PD +DESLSLCDLP+NL+K+DNP+ L E KPRA +    AAD++FDFGSL G
Sbjct: 11  EDELYMEEPDEKDESLSLCDLPINLVKDDNPHFLLEIKPRAGKASIAAADDDFDFGSLGG 70

Query: 74  FSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRSVNSS 133
           FSN A QPPPEMCLADEV YQGQLLP+RLSVSSDN+SAG+YRRH          RS+NSS
Sbjct: 71  FSNLADQPPPEMCLADEVFYQGQLLPLRLSVSSDNTSAGVYRRH---------LRSINSS 130

Query: 134 GSSSSRRSQCSSSSI-----TSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFRQASTG 193
           GSSSS RSQCSSSS+     T+TSAAR+ R RV NLFHSHPSPKPQIKA+P T RQASTG
Sbjct: 131 GSSSS-RSQCSSSSVTNNSLTTTSAARDPRLRVPNLFHSHPSPKPQIKASPITSRQASTG 190

Query: 194 GRSQKSS-TWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGGGGSSD 253
           G  QKSS TWDFFRLGV K PGMEL+DLKHRSSTRR          K+F+P +     S 
Sbjct: 191 GNRQKSSFTWDFFRLGVVKTPGMELEDLKHRSSTRRNSSVSRSNSEKAFVPAAPSNERSV 250

Query: 254 KEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNKITAQA 313
           K    RPR  +LIE RKSS GILSGVGCKCSV STVAP P KA+VVVRR G+   I A++
Sbjct: 251 KVMMRRPRESVLIE-RKSS-GILSGVGCKCSVGSTVAPAP-KAMVVVRRKGNGKNIPAKS 310

Query: 314 ATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHATLPCD 354
                           ELRMKK+ KEKE++EKL LSRHRTFEWLKE    LSHA   C+
Sbjct: 311 ----------------ELRMKKLGKEKEEEEKLELSRHRTFEWLKE----LSHANFSCE 336

BLAST of Moc01g25890 vs. ExPASy TrEMBL
Match: A0A6J1GEW9 (uncharacterized protein LOC111453545 OS=Cucurbita moschata OX=3662 GN=LOC111453545 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 1.1e-100
Identity = 228/362 (62.98%), Postives = 254/362 (70.17%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRA----VETAADEEFDF 69
           E W E   YMEQ DHEDE+LSLCDLP++++K D P+ LFE+KP A    +  A D+EFDF
Sbjct: 8   EGWGEDEFYMEQLDHEDETLSLCDLPIHVVKADKPHFLFEEKPGAGEKPIAAADDDEFDF 67

Query: 70  GSLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFR 129
           GSL GFSNS+AQPPP+MCLADEV YQG+LLPVRLSVSSD +S G +RR GSG GS G  R
Sbjct: 68  GSLGGFSNSSAQPPPQMCLADEVFYQGKLLPVRLSVSSDTTSTGFHRRQGSGYGSFGNVR 127

Query: 130 SVNSSGSSSSRRSQCSSS-----SITSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTFR 189
           S+NSSGSSSS RSQCSSS     SITSTSA RNS PRV NLFHSHPSPKPQIKA+P TFR
Sbjct: 128 SINSSGSSSS-RSQCSSSSVTTTSITSTSAVRNSLPRVPNLFHSHPSPKPQIKASPITFR 187

Query: 190 QAST-GGRSQKSSTWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSGG 249
           QAST GGR   SS WDFFRLGV KAPGMELQDLKHRSSTRR          K+FIP +  
Sbjct: 188 QASTGGGRQISSSKWDFFRLGVVKAPGMELQDLKHRSSTRRNSSVSRSDSEKAFIP-AAS 247

Query: 250 GGSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNK 309
            G+S KE   RPRA + IE ++  GGILSGVGCKCSV STV P P K VVV RR G+   
Sbjct: 248 NGNSVKEIMRRPRASVSIERKR--GGILSGVGCKCSVGSTVTPAP-KTVVVRRRKGN--- 307

Query: 310 ITAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHAT 352
                                  R K  +KEKE+QEKLALSR RTFEWLKE    LSHA+
Sbjct: 308 -----------------------RKKTPKKEKEEQEKLALSRDRTFEWLKE----LSHAS 334

BLAST of Moc01g25890 vs. ExPASy TrEMBL
Match: A0A6J1IVT1 (uncharacterized protein LOC111478847 OS=Cucurbita maxima OX=3661 GN=LOC111478847 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 7.6e-99
Identity = 226/363 (62.26%), Postives = 253/363 (69.70%), Query Frame = 0

Query: 10  ETWSEGGVYMEQPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVETAA-----DEEFD 69
           E W E   YMEQ DHEDE+LSLCDLP++++K++ P+ LFE+KP A E        DEEFD
Sbjct: 8   EGWGEDEFYMEQLDHEDETLSLCDLPIHVVKDNKPHFLFEEKPGAGENPIAAVDDDEEFD 67

Query: 70  FGSLWGFSNSAAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKF 129
           FGSL GFSNS+AQPPP+MCLADEV +QG+LLPVRLSVSSD +S G +RR GSG GS G  
Sbjct: 68  FGSLGGFSNSSAQPPPQMCLADEVFHQGKLLPVRLSVSSDTTSTGFHRRQGSGYGSFGNV 127

Query: 130 RSVNSSGSSSSRRSQCSSS-----SITSTSAARNSRPRVTNLFHSHPSPKPQIKAAPTTF 189
           RS+NSSGSSSS RSQCSSS     SITSTSA RNS PRV NLFHSHPSPKPQIKA+P TF
Sbjct: 128 RSINSSGSSSS-RSQCSSSSVTTTSITSTSAVRNSLPRVPNLFHSHPSPKPQIKASPITF 187

Query: 190 RQASTGGRSQKS-STWDFFRLGVAKAPGMELQDLKHRSSTRR----------KSFIPTSG 249
           RQASTGG  Q S S WDFFRLGV KAPGMELQDLKHRSSTRR          K+FIP + 
Sbjct: 188 RQASTGGGRQISPSKWDFFRLGVVKAPGMELQDLKHRSSTRRNSSVSRSDSEKAFIP-AA 247

Query: 250 GGGSSDKEFTMRPRARILIETRKSSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSN 309
             G+S KE   RPRA + IE ++  GGILSGVGCKCSV STV P PK   V VRR   ++
Sbjct: 248 SNGNSVKEIMRRPRASVSIERKR--GGILSGVGCKCSVGSTVTPAPK--TVEVRRRKGNS 307

Query: 310 KITAQAATATATATAIREKAMRELRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSHA 352
           K T                          EKEKE+QEKLALSR RTFEWLKE    LSHA
Sbjct: 308 KNTP-------------------------EKEKEEQEKLALSRDRTFEWLKE----LSHA 335

BLAST of Moc01g25890 vs. TAIR 10
Match: AT5G67350.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 89.7 bits (221), Expect = 5.0e-18
Identity = 97/336 (28.87%), Postives = 150/336 (44.64%), Query Frame = 0

Query: 21  QPDHEDESLSLCDLPLNLIKEDNPNSLFEKKPRAVETAADEEFDFGSLWGFSNS------ 80
           + + E+E+LSLCDLP             + + R++    DEEFD G  +G  +S      
Sbjct: 27  EEEEEEEALSLCDLPNE-----------KGELRSIVKEEDEEFDSGFEFGIGSSFRAGSD 86

Query: 81  AAQPPPEMCLADEVIYQGQLLPVRLSVSSDNSSAGLYRRHGSGSGSVGKFRSVNSSGSSS 140
           + +P PEM  ADE+ ++G++LP+R SVS D   AGL              R +  S S  
Sbjct: 87  SCEPAPEMSTADELFFKGRILPLRHSVSLD---AGLNE----------PTRLITRSESVE 146

Query: 141 SRRSQCSSSSITSTSAARNSRPRVTNLF-HSHPSPKPQIKAAPTTFRQASTGGRSQKSST 200
            RR          T   R+ R    N   +S PSP+PQI+ + +   + ++    + SS 
Sbjct: 147 FRR----------TGIIRSDRKIKNNFIDYSQPSPQPQIRRSSSMTARVNSIRNPKSSSI 206

Query: 201 WDFFRLGVAKAPGMELQDL--KHRSSTRRKSFIPTSGGGGSSDKEFTMRPRARILIETRK 260
           WDF RLG+ + P +EL+      + S  R S   ++    +S K  +   R+R     R+
Sbjct: 207 WDFLRLGLVRTPEIELRTTAGNAKLSVSRNSSCSSTSTSSNSKKIGSGESRSR----NRR 266

Query: 261 SSGGILSGVGCKCSVESTVAPEPKKAVVVVRRNGSSNKITAQAATATATATAIREKAMRE 320
            S        CKCSV +    E K A V ++                  ++   E+  R 
Sbjct: 267 RS---FMFSDCKCSVST----ETKMAPVKIK-----------------VSSGETEEKQRM 296

Query: 321 LRMKKVEKEKEQQEKLALSRHRTFEWLKELSLSLSH 348
           +  K  +KE    EK A++R RTFEWL ++   + H
Sbjct: 327 MEKKTAKKE----EKSAMARKRTFEWLSQVGFVVDH 296