Moc01g25560 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g25560
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cysteine proteinase COT44
Location	chr1: 18246755 .. 18249642 (+)
RNA-Seq Expression	Moc01g25560
Synteny	Moc01g25560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACCGCCACTTCGCTCGCCGTCCTCTCCTTCTTCTTCTTCTTCCTTCTTTCCGCCCTCGCCTCCGCCTCGGACATGTCCGTGCTCAGCCGCCGGAGAGACGGCGAGGTGAGAGAAATCTACGACCTGTGGCTGGCGAAGCACGGCAAGGCCTACAACGGAATCGAAGAACGGGAGAAGAGATTCCAGATCTTCAAGGACAATCTCAACTTCATCGATGAGCATAATTCCGAGAATCGGACGTATAAGGTCGGATTGAACAGGTTCGCGGATTTGAGCAACGACGAGTATCGGGCCGCGTATTTGGGGACCAGGTCGAATCCCGTGCGCAGAGTCATGAAGGCGATGAAGGCCAGCCGCCGATACACCGTCAACGCCGGCGATCAGTTGCCGGAATCCGTCGATTGGAGGGCGAGAGGAGCCGTTGGTCCCATCAAAGATCAAGGAAGTTGCGGTGAGTTTTTTAAATATCAACCTCTGTGGAGAGTCGATACTCTTTCGAAATAATAATTTAAAATAAAAACATATATATATATATATTTTTTCTTGTACAATATCGGGGATATATATTTTCTTGTAAGTTGCTTTTCGTGTATTTTCAGAAATTTTAATCTGTTTTATACGGTTCAGAAATAAAACGATTGTTTAGGCATTTTTTTTAAATCCCTTTTTATTGTTAATCTATTTTCTGTTTCTTTATAAAACGTGTTTTTATACTATTTTTTAATTTTTTAAAAAGTTTTATGTACTATTGTTTTTTCTGTTTTTTTTTGTATCTTCATTTATTTTTAAAATTTATCTAAAATAACAACAATTTTGTATCTTTATTTTCAGATAATATTGATAGGTAAAAAATTTACTTCAAAAAAATACACATGAAAGCAATAAATATTAATCTATTTTTCCTTTCTTTTTTTTAAATATATTTTAGTAGCAAAGAGAAGCACTACTTTCATTTCAACTATTAAAAATTAGTTAAATGAAAAACTATAAAATATTAATATTAATAATTTTTTTTAAATTAATTTATTTTGAATAGTGAAAGCTCCTTGAATAGATACGTATTTCATTTTTTAAAATCTGAAGACTGATTTTAAAATCAAACCAAATGTATTTTTTATATTAATATTTTGAATAAACTATCTAATTGAAAATCTAAAGTAAGCTTTTAAACTTTATTTTCATGCTTGAAGTTTCTTTATTTATTCTTTTTTTTTAAATTATTTATTTTCCCTTTAAAATAAGATAATTAAACATTGTTTTCTAAAATAAGATAAAATAAGAAAGAAAAGAGAGATAAAAGGGTTTTTTTAGGAAAAAAAATGAAAAGAAAAATATGTGATACAAAGTACGGAACTTTTTTTGATTAATAATAGTTTAATTGACTGAAAAAATCAATTTAGAAAGGCTGAATAATTTTATGATCTAAAAAATTTATTCAGAATTCAAACATATAAAAGAGCAATGAACTTTTGGCCTTATAACGAATCAACATTTATAATTTTTGTTGTGTAAAAAAAAAACACTTATGATTTTTGTAGGCTTTAACAACTTTATTGTTCAGATACTGTAGTCACCAATGATTAATTAAAGATTAATCACAATCATTCAGAGGTGTAGAAAAATGGAAGATCCCCTTCGTAATATGACTTGTTTTATTTACTTAAATTGTGAAAGATCGTGTAAGAGATATAAGTTGCATTTCGTTTCAAAAAAAAAATATACAGAGAGAGATATTCTTGAATTTGGAGCATAATATAATTAATGGCGGACCTTGGCATTCTGCAAAATCCAATTACTGTTTGAAACCAGTGAGGCCATTAATGCGAAATGTTTGAGCTCTGAATTTGGAACTGTGTTGGGAAAACAGGAAGCTGCTGGGCATTCTCGACCATAGCAGCTGTGGAAGGCATAAACCAGATCGTCACCGGAAAACTGATCTCCCTCTCGGAGCAAGAGCTTGTTAGCTGTGACACAGATTATAATTCAGGCTGCAATGGAGGTCTTATGGACTATGCCTTCCAGTTCATCATTGACAATGGCGGCTTGGACACCGAGGAAGATTACCCTTATGAAGGTCTCGACGGACAATGCGATCCTACCAGGGTGAGGCTGTCCTCCCACATATCTGCTTTTGTGTTCAACTTTTACTTGGCTTGGTCTTAACTCTTAACTCTTGAGTTTCCTCTCTGACGTTGGATGCCATCTCTCAGAAAAATGCCAAGGTTGTTAGCATTGATGGGTACGAGGATGTCCCTGTCGGCGACGAGGAAGCGTTGAAGAAGGCCGTTGCTCATCAGCCCGTGAGCGTCGCCATTGAAGCCGGTGGCAGGGCTTTGCAACTCTACCAGTCTGTAAGCAAATCTCTCTTTTTCTTTCTCAGATTAGAGTCTTCTTGATTCTTTATCACTCCACGGATCCAAGTCCATTATAAATACTTCTGTCCATGATCCTCACCACTTCAAAATTATGTGAAACTTTCAAACCAAAAGATCTGAAAACCTTGTTTGAAATGCCTATCTATATTTCCCTCCCCCAAGGTTGATCCACAGCTCACCTTATCAACTTGTGATTGATCAATATATAATCCAACTTTTCAGGGGGTGTTTACTGGCAAGTGCGGTTCAGATCTTGACCATGGTGTGGTCGCTGTCGGGTATGGCACAGAAAACGGAGTTGATTATTGGCTTGTAAGGAACTCATGGGGCACAGAGTGGGGTGAGGATGGCTATTTCAGGCTAGAGCGCAATGTGAAGAACACATCCAGTGGCAAGTGTGGAATTACAATGGAGTCTTCTTACCCTGTTAAGACTAGCGACAACCCAACGGGTTCATACTTAGGTTTGGAAAGAGCTGGGGACAGGATCAAGATTAGCAGTGCTTGA

mRNA sequence

ATGGCCACCGCCACTTCGCTCGCCGTCCTCTCCTTCTTCTTCTTCTTCCTTCTTTCCGCCCTCGCCTCCGCCTCGGACATGTCCGTGCTCAGCCGCCGGAGAGACGGCGAGGTGAGAGAAATCTACGACCTGTGGCTGGCGAAGCACGGCAAGGCCTACAACGGAATCGAAGAACGGGAGAAGAGATTCCAGATCTTCAAGGACAATCTCAACTTCATCGATGAGCATAATTCCGAGAATCGGACGTATAAGGTCGGATTGAACAGGTTCGCGGATTTGAGCAACGACGAGTATCGGGCCGCGTATTTGGGGACCAGGTCGAATCCCGTGCGCAGAGTCATGAAGGCGATGAAGGCCAGCCGCCGATACACCGTCAACGCCGGCGATCAGTTGCCGGAATCCGTCGATTGGAGGGCGAGAGGAGCCGTTGGTCCCATCAAAGATCAAGGAAGTTGCGGAAGCTGCTGGGCATTCTCGACCATAGCAGCTGTGGAAGGCATAAACCAGATCGTCACCGGAAAACTGATCTCCCTCTCGGAGCAAGAGCTTGTTAGCTGTGACACAGATTATAATTCAGGCTGCAATGGAGGTCTTATGGACTATGCCTTCCAGTTCATCATTGACAATGGCGGCTTGGACACCGAGGAAGATTACCCTTATGAAGGTCTCGACGGACAATGCGATCCTACCAGGAAAAATGCCAAGGTTGTTAGCATTGATGGGTACGAGGATGTCCCTGTCGGCGACGAGGAAGCGTTGAAGAAGGCCGTTGCTCATCAGCCCGTGAGCGTCGCCATTGAAGCCGGTGGCAGGGCTTTGCAACTCTACCAGTCTGGGGTGTTTACTGGCAAGTGCGGTTCAGATCTTGACCATGGTGTGGTCGCTGTCGGGTATGGCACAGAAAACGGAGTTGATTATTGGCTTGTAAGGAACTCATGGGGCACAGAGTGGGGTGAGGATGGCTATTTCAGGCTAGAGCGCAATGTGAAGAACACATCCAGTGGCAAGTGTGGAATTACAATGGAGTCTTCTTACCCTGTTAAGACTAGCGACAACCCAACGGGTTCATACTTAGGTTTGGAAAGAGCTGGGGACAGGATCAAGATTAGCAGTGCTTGA

Coding sequence (CDS)

ATGGCCACCGCCACTTCGCTCGCCGTCCTCTCCTTCTTCTTCTTCTTCCTTCTTTCCGCCCTCGCCTCCGCCTCGGACATGTCCGTGCTCAGCCGCCGGAGAGACGGCGAGGTGAGAGAAATCTACGACCTGTGGCTGGCGAAGCACGGCAAGGCCTACAACGGAATCGAAGAACGGGAGAAGAGATTCCAGATCTTCAAGGACAATCTCAACTTCATCGATGAGCATAATTCCGAGAATCGGACGTATAAGGTCGGATTGAACAGGTTCGCGGATTTGAGCAACGACGAGTATCGGGCCGCGTATTTGGGGACCAGGTCGAATCCCGTGCGCAGAGTCATGAAGGCGATGAAGGCCAGCCGCCGATACACCGTCAACGCCGGCGATCAGTTGCCGGAATCCGTCGATTGGAGGGCGAGAGGAGCCGTTGGTCCCATCAAAGATCAAGGAAGTTGCGGAAGCTGCTGGGCATTCTCGACCATAGCAGCTGTGGAAGGCATAAACCAGATCGTCACCGGAAAACTGATCTCCCTCTCGGAGCAAGAGCTTGTTAGCTGTGACACAGATTATAATTCAGGCTGCAATGGAGGTCTTATGGACTATGCCTTCCAGTTCATCATTGACAATGGCGGCTTGGACACCGAGGAAGATTACCCTTATGAAGGTCTCGACGGACAATGCGATCCTACCAGGAAAAATGCCAAGGTTGTTAGCATTGATGGGTACGAGGATGTCCCTGTCGGCGACGAGGAAGCGTTGAAGAAGGCCGTTGCTCATCAGCCCGTGAGCGTCGCCATTGAAGCCGGTGGCAGGGCTTTGCAACTCTACCAGTCTGGGGTGTTTACTGGCAAGTGCGGTTCAGATCTTGACCATGGTGTGGTCGCTGTCGGGTATGGCACAGAAAACGGAGTTGATTATTGGCTTGTAAGGAACTCATGGGGCACAGAGTGGGGTGAGGATGGCTATTTCAGGCTAGAGCGCAATGTGAAGAACACATCCAGTGGCAAGTGTGGAATTACAATGGAGTCTTCTTACCCTGTTAAGACTAGCGACAACCCAACGGGTTCATACTTAGGTTTGGAAAGAGCTGGGGACAGGATCAAGATTAGCAGTGCTTGA

Protein sequence

MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEEREKRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASRRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGTENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGLERAGDRIKISSA

Homology

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BLAST of Moc01g25560 vs. NCBI nr
Match: XP_022132954.1 (cysteine proteinase COT44 [Momordica charantia])

HSP 1 Score: 745.7 bits (1924), Expect = 1.9e-211
Identity = 371/372 (99.73%), Postives = 371/372 (99.73%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE
Sbjct: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKA KAS
Sbjct: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAXKAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE
Sbjct: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID
Sbjct: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT
Sbjct: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGL 360
           ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGL
Sbjct: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGL 360

Query: 361 ERAGDRIKISSA 373
           ERAGDRIKISSA
Sbjct: 361 ERAGDRIKISSA 372

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BLAST of Moc01g25560 vs. NCBI nr
Match: XP_023518142.1 (cysteine proteinase COT44-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.4 bits (1596), Expect = 2.0e-173
Identity = 310/374 (82.89%), Postives = 333/374 (89.04%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MA AT+   L+F  FF+LS       +S L++R DGEVREIYD+WLAKHGKAYNGIEERE
Sbjct: 1   MALATTF--LAFLSFFVLS-------ISALNQRTDGEVREIYDIWLAKHGKAYNGIEERE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRF IFKDNLNFIDEHNS+NRTY VGLN FADL+N+EYRA +LGTRS+P RRVMKA  AS
Sbjct: 61  KRFLIFKDNLNFIDEHNSQNRTYTVGLNMFADLTNEEYRATFLGTRSHPARRVMKAKSAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRY VN  D+LPESVDWR RGAV PIK+QGSCGSCWAFSTIAAVEGINQIVTG+LISLSE
Sbjct: 121 RRYAVNDADRLPESVDWRTRGAVAPIKNQGSCGSCWAFSTIAAVEGINQIVTGELISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDT YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTR+NAKVVSID
Sbjct: 181 QELVSCDTKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRENAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYGT
Sbjct: 241 GYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVK--TSDNPTGSYL 360
           ENGVDYWLVRNSWGT WGEDGYF+LERNVK+T+ GKCGI ME+SYPVK   ++NPTGSYL
Sbjct: 301 ENGVDYWLVRNSWGTGWGEDGYFKLERNVKHTTDGKCGIAMEASYPVKNGNNNNPTGSYL 360

Query: 361 GLERAGDRIKISSA 373
           GLE AGD+ KISSA
Sbjct: 361 GLELAGDKNKISSA 365

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BLAST of Moc01g25560 vs. NCBI nr
Match: KAG7027046.1 (Oryzain alpha chain [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.5 bits (1591), Expect = 7.7e-173
Identity = 308/374 (82.35%), Postives = 333/374 (89.04%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MA AT+   L+F  FF+LS       +S L++R DGEVREIYD+WLAKHGKAYNGIEERE
Sbjct: 1   MALATTF--LAFLSFFVLS-------ISALNQRTDGEVREIYDIWLAKHGKAYNGIEERE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRF IFKDNLNF+DEHNS+NRTY VGLN FADL+N+EYRA +LGTRS+P RRVMKA  AS
Sbjct: 61  KRFLIFKDNLNFVDEHNSQNRTYTVGLNMFADLTNEEYRATFLGTRSHPARRVMKAKSAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRY VN  D+LPESVDWR +GAV PIK+QGSCGSCWAFSTIAAVEGINQIVTG+LISLSE
Sbjct: 121 RRYAVNDDDRLPESVDWRTKGAVAPIKNQGSCGSCWAFSTIAAVEGINQIVTGELISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDT YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTR+NAKVVSID
Sbjct: 181 QELVSCDTKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRENAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYGT
Sbjct: 241 GYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVK--TSDNPTGSYL 360
           ENGVDYWLVRNSWGT WGEDGYF+LERNVK+T+ GKCGI ME+SYPVK   ++NPTGSYL
Sbjct: 301 ENGVDYWLVRNSWGTGWGEDGYFKLERNVKHTTDGKCGIAMEASYPVKNGNNNNPTGSYL 360

Query: 361 GLERAGDRIKISSA 373
           GLE AGD+ KISSA
Sbjct: 361 GLELAGDKNKISSA 365

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BLAST of Moc01g25560 vs. NCBI nr
Match: XP_022963075.1 (zingipain-2-like [Cucurbita moschata])

HSP 1 Score: 617.1 bits (1590), Expect = 1.0e-172
Identity = 308/374 (82.35%), Postives = 333/374 (89.04%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MA AT+   L+F  FF+LS       +S L++R DGEVREIYD+WLAKHGKAYNGIEERE
Sbjct: 1   MALATTF--LAFLSFFVLS-------ISALNQRTDGEVREIYDIWLAKHGKAYNGIEERE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRF IFKDNLNF+DEHNS+NRTY VGLN FADL+N+EYRA +LGTRS+P RRVMKA  AS
Sbjct: 61  KRFLIFKDNLNFLDEHNSQNRTYTVGLNMFADLTNEEYRATFLGTRSHPARRVMKAKSAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRY VN  D+LPESVDWR +GAV PIK+QGSCGSCWAFSTIAAVEGINQIVTG+LISLSE
Sbjct: 121 RRYAVNDDDRLPESVDWRTKGAVAPIKNQGSCGSCWAFSTIAAVEGINQIVTGELISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDT YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTR+NAKVVSID
Sbjct: 181 QELVSCDTKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRENAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYGT
Sbjct: 241 GYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVK--TSDNPTGSYL 360
           ENGVDYWLVRNSWGT WGEDGYF+LERNVK+T+ GKCGI ME+SYPVK   ++NPTGSYL
Sbjct: 301 ENGVDYWLVRNSWGTGWGEDGYFKLERNVKHTTDGKCGIAMEASYPVKNGNNNNPTGSYL 360

Query: 361 GLERAGDRIKISSA 373
           GLE AGD+ KISSA
Sbjct: 361 GLELAGDKNKISSA 365

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BLAST of Moc01g25560 vs. NCBI nr
Match: XP_023544321.1 (cysteine proteinase COT44-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615.9 bits (1587), Expect = 2.2e-172
Identity = 302/364 (82.97%), Postives = 323/364 (88.74%), Query Frame = 0


Query: 9   VLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEEREKRFQIFKD 68
           +L    FFLLS  A +S  S+LSRR D EVR  YDLWLAK GK YNGIEE+EKRFQIF++
Sbjct: 7   LLPLLSFFLLSTSALSSSSSLLSRRSDSEVRATYDLWLAKFGKTYNGIEEQEKRFQIFRE 66

Query: 69  NLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASRRYTVNAG 128
           NLNFID+HNSENRTYKVGLNRFADL+NDEYRAAYLGTRS+P RRVMK   ASRRY VN  
Sbjct: 67  NLNFIDDHNSENRTYKVGLNRFADLTNDEYRAAYLGTRSSPARRVMKDKNASRRYAVNNR 126

Query: 129 DQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQELVSCDT 188
           DQLPESVDWRA+GAV PIK+QGSCGSCWAFSTIAAVEGINQIVTG+LISLSEQELVSCD 
Sbjct: 127 DQLPESVDWRAKGAVTPIKNQGSCGSCWAFSTIAAVEGINQIVTGELISLSEQELVSCDK 186

Query: 189 DYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDGYEDVPVG 248
            YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDGYEDVP  
Sbjct: 187 KYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDGYEDVPAN 246

Query: 249 DEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGTENGVDYWL 308
           DEE LKKAVAHQPVSVAIEA G A+QLYQSGVFTG CGS LDHGVVAVGYGTENGVDYWL
Sbjct: 247 DEETLKKAVAHQPVSVAIEASGLAMQLYQSGVFTGDCGSALDHGVVAVGYGTENGVDYWL 306

Query: 309 VRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGLERAGDRIK 368
           VRNSWGT WGEDGYF+LERNVK+T++GKCGITM++SYPVK   NPTGSY  LE  GD+ K
Sbjct: 307 VRNSWGTGWGEDGYFKLERNVKHTTNGKCGITMQASYPVKNGSNPTGSYSALETVGDKNK 366

Query: 369 ISSA 373
           IS+A
Sbjct: 367 ISTA 370

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BLAST of Moc01g25560 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 9.9e-131
Identity = 223/352 (63.35%), Postives = 268/352 (76.14%), Query Frame = 0


Query: 15  FFLLSALASASDMSVLS-----------RRRDGEVREIYDLWLAKHGKA--YNGIEEREK 74
           F  + A++SA DMS++S            R + EV  IY+ WL KHGKA   N + E+++
Sbjct: 12  FLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDR 71

Query: 75  RFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASR 134
           RF+IFKDNL F+DEHN +N +Y++GL RFADL+NDEYR+ YLG +           + S 
Sbjct: 72  RFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEK----KGERRTSL 131

Query: 135 RYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQ 194
           RY    GD+LPES+DWR +GAV  +KDQG CGSCWAFSTI AVEGINQIVTG LI+LSEQ
Sbjct: 132 RYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQ 191

Query: 195 ELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDG 254
           ELV CDT YN GCNGGLMDYAF+FII NGG+DT++DYPY+G+DG CD  RKNAKVV+ID 
Sbjct: 192 ELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVTIDS 251

Query: 255 YEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGTE 314
           YEDVP   EE+LKKAVAHQP+S+AIEAGGRA QLY SG+F G CG+ LDHGVVAVGYGTE
Sbjct: 252 YEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGYGTE 311

Query: 315 NGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNP 354
           NG DYW+VRNSWG  WGE GY R+ RN+  +SSGKCGI +E SYP+K  +NP
Sbjct: 312 NGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGENP 358

[Close]

BLAST of Moc01g25560 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.6e-128
Identity = 223/345 (64.64%), Postives = 264/345 (76.52%), Query Frame = 0


Query: 24  ASDMSVLS-----------RRRDGEVREIYDLWLAKHGKA---YNGI-EEREKRFQIFKD 83
           A DMS++S            R D EV  IY+ W+ +HGK     NG+  E+++RF+IFKD
Sbjct: 21  AMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKD 80

Query: 84  NLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASRRYTVNAG 143
           NL FIDEHN++N +YK+GL RFADL+N+EYR+ YLG +  P +RV   +K S RY    G
Sbjct: 81  NLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK--PTKRV---LKTSDRYQARVG 140

Query: 144 DQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQELVSCDT 203
           D LP+SVDWR  GAV  +KDQGSCGSCWAFSTI AVEGIN+IVTG LISLSEQELV CDT
Sbjct: 141 DALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSEQELVDCDT 200

Query: 204 DYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDGYEDVPVG 263
            YN GCNGGLMDYAF+FII NGG+DTE DYPY+  DG+CD  RKNAKVV+ID YEDVP  
Sbjct: 201 SYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTIDSYEDVPEN 260

Query: 264 DEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGTENGVDYWL 323
            E +LKKA+AHQP+SVAIEAGGRA QLY SGVF G CG++LDHGVVAVGYGTENG DYW+
Sbjct: 261 SEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYGTENGKDYWI 320

Query: 324 VRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNP 354
           VRNSWG  WGE GY ++ RN++   +GKCGI ME+SYP+K   NP
Sbjct: 321 VRNSWGNRWGESGYIKMARNIE-APTGKCGIAMEASYPIKKGQNP 359

[Close]

BLAST of Moc01g25560 vs. ExPASy Swiss-Prot
Match: Q94B08 (Germination-specific cysteine protease 1 OS=Arabidopsis thaliana OX=3702 GN=GCP1 PE=2 SV=2)

HSP 1 Score: 458.0 bits (1177), Expect = 1.0e-127
Identity = 226/366 (61.75%), Postives = 275/366 (75.14%), Query Frame = 0


Query: 4   ATSLAVLSFFFFFLLSALASASDMSVLSR---------RRDGEVREIYDLWLAKHGKAYN 63
           A S  VLS    +++ +LAS  +  +            R D EVR IY  W A+HGK  N
Sbjct: 2   APSTKVLSLLLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNN 61

Query: 64  G----IEEREKRFQIFKDNLNFIDEHNSENR--TYKVGLNRFADLSNDEYRAAYLGTRSN 123
                I +++KRF IFKDNL FID HN +N+  TYK+GL +F DL+NDEYR  YLG R+ 
Sbjct: 62  NNNGIINDQDKRFNIFKDNLRFIDLHNEDNKNATYKLGLTKFTDLTNDEYRKLYLGARTE 121

Query: 124 PVRRVMKAMKASRRYTVNA-GDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGI 183
           P RR+ KA   +++Y+    G ++PE+VDWR +GAV PIKDQG+CGSCWAFST AAVEGI
Sbjct: 122 PARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGI 181

Query: 184 NQIVTGKLISLSEQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQC 243
           N+IVTG+LISLSEQELV CD  YN GCNGGLMDYAFQFI+ NGGL+TE+DYPY G  G+C
Sbjct: 182 NKIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKC 241

Query: 244 DPTRKNAKVVSIDGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGS 303
           +   KN++VVSIDGYEDVP  DE ALKKA+++QPVSVAIEAGGR  Q YQSG+FTG CG+
Sbjct: 242 NSFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGT 301

Query: 304 DLDHGVVAVGYGTENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPV 354
           +LDH VVAVGYG+ENGVDYW+VRNSWG  WGE+GY R+ERN+  + SGKCGI +E+SYPV
Sbjct: 302 NLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPV 361

[Close]

BLAST of Moc01g25560 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 450.7 bits (1158), Expect = 1.6e-125
Identity = 225/354 (63.56%), Postives = 268/354 (75.71%), Query Frame = 0


Query: 6   SLAVLSFFFFFLLSALASASDMSVLS--RRRDGEVREIYDLWLAKHGKAYNGIEEREKRF 65
           S+A+ +     LLS   +A+DMS++S   R + E R +Y  W A+HGK+YN + E E+R+
Sbjct: 4   SMALAAAALLLLLS--LAAADMSIVSYGERSEEEARRLYAEWKAEHGKSYNAVGEEERRY 63

Query: 66  QIFKDNLNFIDEHNSEN----RTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKA 125
             F+DNL +IDEHN+       ++++GLNRFADL+N+EYR  YLG R+ P R      K 
Sbjct: 64  AAFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYLGLRNKPRRE----RKV 123

Query: 126 SRRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLS 185
           S RY     + LPESVDWR +GAV  IKDQG CGSCWAFS IAAVEGINQIVTG LISLS
Sbjct: 124 SDRYLAADNEALPESVDWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLS 183

Query: 186 EQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSI 245
           EQELV CDT YN GCNGGLMDYAF FII+NGG+DTE+DYPY+G D +CD  RKNAKVV+I
Sbjct: 184 EQELVDCDTSYNEGCNGGLMDYAFDFIINNGGIDTEDDYPYKGKDERCDVNRKNAKVVTI 243

Query: 246 DGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYG 305
           D YEDV    E +L+KAVA+QPVSVAIEAGGRA QLY SG+FTGKCG+ LDHGV AVGYG
Sbjct: 244 DSYEDVTPNSETSLQKAVANQPVSVAIEAGGRAFQLYSSGIFTGKCGTALDHGVAAVGYG 303

Query: 306 TENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNP 354
           TENG DYW+VRNSWG  WGE GY R+ERN+K  SSGKCGI +E SYP+K  +NP
Sbjct: 304 TENGKDYWIVRNSWGKSWGESGYVRMERNIK-ASSGKCGIAVEPSYPLKKGENP 350

[Close]

BLAST of Moc01g25560 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.3e-124
Identity = 222/357 (62.18%), Postives = 274/357 (76.75%), Query Frame = 0


Query: 1   MATA-TSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEER 60
           MAT+  S+ +    F  LL +L+  S  +  + R + E R +Y+ WL ++ K YNG+ E+
Sbjct: 1   MATSIKSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEK 60

Query: 61  EKRFQIFKDNLNFIDEHNS-ENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMK 120
           E+RF+IFKDNL F++EH+S  NRTY+VGL RFADL+NDE+RA YL ++    R  +K  K
Sbjct: 61  ERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMERTRVPVKGEK 120

Query: 121 ASRRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISL 180
               Y    GD LP+++DWRA+GAV P+KDQGSCGSCWAFS I AVEGINQI TG+LISL
Sbjct: 121 ----YLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISL 180

Query: 181 SEQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLD-GQCDPTRKNAKVV 240
           SEQELV CDT YN GC GGLMDYAF+FII+NGG+DTEEDYPY   D   C+  +KN +VV
Sbjct: 181 SEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVV 240

Query: 241 SIDGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVG 300
           +IDGYEDVP  DE++LKKA+A+QP+SVAIEAGGRA QLY SGVFTG CG+ LDHGVVAVG
Sbjct: 241 TIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVG 300

Query: 301 YGTENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTS-DNP 354
           YG+E G DYW+VRNSWG+ WGE GYF+LERN+K  SSGKCG+ M +SYP K+S  NP
Sbjct: 301 YGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE-SSGKCGVAMMASYPTKSSGSNP 352

[Close]

BLAST of Moc01g25560 vs. ExPASy TrEMBL
Match: A0A6J1BUJ2 (cysteine proteinase COT44 OS=Momordica charantia OX=3673 GN=LOC111005671 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.1e-212
Identity = 371/372 (99.73%), Postives = 371/372 (99.73%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE
Sbjct: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKA KAS
Sbjct: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAXKAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE
Sbjct: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID
Sbjct: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT
Sbjct: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGL 360
           ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGL
Sbjct: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLGL 360

Query: 361 ERAGDRIKISSA 373
           ERAGDRIKISSA
Sbjct: 361 ERAGDRIKISSA 372

[Close]

BLAST of Moc01g25560 vs. ExPASy TrEMBL
Match: A0A6J1HGZ6 (zingipain-2-like OS=Cucurbita moschata OX=3662 GN=LOC111463384 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.9e-173
Identity = 308/374 (82.35%), Postives = 333/374 (89.04%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MA AT+   L+F  FF+LS       +S L++R DGEVREIYD+WLAKHGKAYNGIEERE
Sbjct: 1   MALATTF--LAFLSFFVLS-------ISALNQRTDGEVREIYDIWLAKHGKAYNGIEERE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRF IFKDNLNF+DEHNS+NRTY VGLN FADL+N+EYRA +LGTRS+P RRVMKA  AS
Sbjct: 61  KRFLIFKDNLNFLDEHNSQNRTYTVGLNMFADLTNEEYRATFLGTRSHPARRVMKAKSAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRY VN  D+LPESVDWR +GAV PIK+QGSCGSCWAFSTIAAVEGINQIVTG+LISLSE
Sbjct: 121 RRYAVNDDDRLPESVDWRTKGAVAPIKNQGSCGSCWAFSTIAAVEGINQIVTGELISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDT YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTR+NAKVVSID
Sbjct: 181 QELVSCDTKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRENAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYGT
Sbjct: 241 GYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVK--TSDNPTGSYL 360
           ENGVDYWLVRNSWGT WGEDGYF+LERNVK+T+ GKCGI ME+SYPVK   ++NPTGSYL
Sbjct: 301 ENGVDYWLVRNSWGTGWGEDGYFKLERNVKHTTDGKCGIAMEASYPVKNGNNNNPTGSYL 360

Query: 361 GLERAGDRIKISSA 373
           GLE AGD+ KISSA
Sbjct: 361 GLELAGDKNKISSA 365

[Close]

BLAST of Moc01g25560 vs. ExPASy TrEMBL
Match: A0A6J1KPD5 (cysteine proteinase COT44-like OS=Cucurbita maxima OX=3661 GN=LOC111497083 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.4e-172
Identity = 309/374 (82.62%), Postives = 330/374 (88.24%), Query Frame = 0


Query: 1   MATATSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEERE 60
           MA AT  A L+F  FF+LS       +S L++R DGEVREIYD+WLAKHGKAYNGIEE E
Sbjct: 1   MALAT--ASLAFLSFFVLS-------ISALNQRSDGEVREIYDMWLAKHGKAYNGIEELE 60

Query: 61  KRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKAS 120
           KRF IFKDNLNFIDEHNS NRTY VGLN FADL+N+EYRAA+LGTRS P RRVMKA  AS
Sbjct: 61  KRFLIFKDNLNFIDEHNSHNRTYTVGLNMFADLTNEEYRAAFLGTRSQPARRVMKAKSAS 120

Query: 121 RRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSE 180
           RRY VN GD+LPESVDWR +GAV PIK+QGSCGSCWAFSTIAAVEGINQIVTG+LISLSE
Sbjct: 121 RRYAVNDGDRLPESVDWRTKGAVAPIKNQGSCGSCWAFSTIAAVEGINQIVTGELISLSE 180

Query: 181 QELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSID 240
           QELVSCDT YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTR+NAKVVSID
Sbjct: 181 QELVSCDTKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRENAKVVSID 240

Query: 241 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 300
           GYEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYGT
Sbjct: 241 GYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYGT 300

Query: 301 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVK--TSDNPTGSYL 360
           ENGVDYWL RNSWGT WGEDGYF+LERNVK+T+ GKCGI ME+SYPVK   ++NPTGSYL
Sbjct: 301 ENGVDYWLARNSWGTGWGEDGYFKLERNVKHTTDGKCGIAMEASYPVKNGNNNNPTGSYL 360

Query: 361 GLERAGDRIKISSA 373
           GLE  GD+ KISSA
Sbjct: 361 GLELGGDKNKISSA 365

[Close]

BLAST of Moc01g25560 vs. ExPASy TrEMBL
Match: A0A5D3CLQ0 (Cysteine proteinase COT44-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004590 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.1e-172
Identity = 311/373 (83.38%), Postives = 328/373 (87.94%), Query Frame = 0


Query: 1   MATA-TSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEER 60
           MATA TSLA+LSFFF   LS  ASA     LSRR DGEVREIYDLWLAKHGKAYNGI+ER
Sbjct: 1   MATATTSLALLSFFF---LSISASA-----LSRRSDGEVREIYDLWLAKHGKAYNGIDER 60

Query: 61  EKRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKA 120
           EKRFQIFK+NLNFID+HNSENRTYKVGLN FADL+NDEYRA YLGTRS P RRVMKA  A
Sbjct: 61  EKRFQIFKENLNFIDDHNSENRTYKVGLNMFADLTNDEYRALYLGTRSPPARRVMKAKTA 120

Query: 121 SRRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLS 180
           SRRY VN  D+LPESVDWRARGAV P+K+QGSCGSCWAFSTIAAVEGINQIVTG LISLS
Sbjct: 121 SRRYAVNNRDRLPESVDWRARGAVAPVKNQGSCGSCWAFSTIAAVEGINQIVTGDLISLS 180

Query: 181 EQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSI 240
           EQELVSCD  YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYE  DGQCDPTRKNAKVVSI
Sbjct: 181 EQELVSCDKKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEAFDGQCDPTRKNAKVVSI 240

Query: 241 DGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYG 300
           D YEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYG
Sbjct: 241 DSYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYG 300

Query: 301 TENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLG 360
            ENGVDYWLVRNSWGT WGEDGYF+LERNVK+T+ GKCGI M++SYPVK  +NPT SYL 
Sbjct: 301 KENGVDYWLVRNSWGTGWGEDGYFKLERNVKHTTEGKCGIAMQASYPVKNDNNPTKSYLS 360

Query: 361 LERAGDRIKISSA 373
           L+   D+ KI+SA
Sbjct: 361 LKSVEDKYKINSA 365

[Close]

BLAST of Moc01g25560 vs. ExPASy TrEMBL
Match: A0A1S3B0U6 (cysteine proteinase COT44-like OS=Cucumis melo OX=3656 GN=LOC103484794 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.1e-172
Identity = 311/373 (83.38%), Postives = 328/373 (87.94%), Query Frame = 0


Query: 1   MATA-TSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEER 60
           MATA TSLA+LSFFF   LS  ASA     LSRR DGEVREIYDLWLAKHGKAYNGI+ER
Sbjct: 1   MATATTSLALLSFFF---LSISASA-----LSRRSDGEVREIYDLWLAKHGKAYNGIDER 60

Query: 61  EKRFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKA 120
           EKRFQIFK+NLNFID+HNSENRTYKVGLN FADL+NDEYRA YLGTRS P RRVMKA  A
Sbjct: 61  EKRFQIFKENLNFIDDHNSENRTYKVGLNMFADLTNDEYRALYLGTRSPPARRVMKAKTA 120

Query: 121 SRRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLS 180
           SRRY VN  D+LPESVDWRARGAV P+K+QGSCGSCWAFSTIAAVEGINQIVTG LISLS
Sbjct: 121 SRRYAVNNRDRLPESVDWRARGAVAPVKNQGSCGSCWAFSTIAAVEGINQIVTGDLISLS 180

Query: 181 EQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSI 240
           EQELVSCD  YNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYE  DGQCDPTRKNAKVVSI
Sbjct: 181 EQELVSCDKKYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEAFDGQCDPTRKNAKVVSI 240

Query: 241 DGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYG 300
           D YEDVP  DEEALKKAVAHQPVSVAIEA G ALQLYQSGVFTGKCGS LDHGVVAVGYG
Sbjct: 241 DSYEDVPANDEEALKKAVAHQPVSVAIEASGLALQLYQSGVFTGKCGSALDHGVVAVGYG 300

Query: 301 TENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNPTGSYLG 360
            ENGVDYWLVRNSWGT WGEDGYF+LERNVK+T+ GKCGI M++SYPVK  +NPT SYL 
Sbjct: 301 KENGVDYWLVRNSWGTGWGEDGYFKLERNVKHTTEGKCGIAMQASYPVKNDNNPTKSYLS 360

Query: 361 LERAGDRIKISSA 373
           L+   D+ KI+SA
Sbjct: 361 LKSVEDKYKINSA 365

[Close]

BLAST of Moc01g25560 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 468.0 bits (1203), Expect = 7.1e-132
Identity = 223/352 (63.35%), Postives = 268/352 (76.14%), Query Frame = 0


Query: 15  FFLLSALASASDMSVLS-----------RRRDGEVREIYDLWLAKHGKA--YNGIEEREK 74
           F  + A++SA DMS++S            R + EV  IY+ WL KHGKA   N + E+++
Sbjct: 12  FLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDR 71

Query: 75  RFQIFKDNLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASR 134
           RF+IFKDNL F+DEHN +N +Y++GL RFADL+NDEYR+ YLG +           + S 
Sbjct: 72  RFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEK----KGERRTSL 131

Query: 135 RYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQ 194
           RY    GD+LPES+DWR +GAV  +KDQG CGSCWAFSTI AVEGINQIVTG LI+LSEQ
Sbjct: 132 RYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQ 191

Query: 195 ELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDG 254
           ELV CDT YN GCNGGLMDYAF+FII NGG+DT++DYPY+G+DG CD  RKNAKVV+ID 
Sbjct: 192 ELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVTIDS 251

Query: 255 YEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGTE 314
           YEDVP   EE+LKKAVAHQP+S+AIEAGGRA QLY SG+F G CG+ LDHGVVAVGYGTE
Sbjct: 252 YEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGYGTE 311

Query: 315 NGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNP 354
           NG DYW+VRNSWG  WGE GY R+ RN+  +SSGKCGI +E SYP+K  +NP
Sbjct: 312 NGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGENP 358

[Close]

BLAST of Moc01g25560 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 459.1 bits (1180), Expect = 3.3e-129
Identity = 223/345 (64.64%), Postives = 264/345 (76.52%), Query Frame = 0


Query: 24  ASDMSVLS-----------RRRDGEVREIYDLWLAKHGKA---YNGI-EEREKRFQIFKD 83
           A DMS++S            R D EV  IY+ W+ +HGK     NG+  E+++RF+IFKD
Sbjct: 21  AMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKD 80

Query: 84  NLNFIDEHNSENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASRRYTVNAG 143
           NL FIDEHN++N +YK+GL RFADL+N+EYR+ YLG +  P +RV   +K S RY    G
Sbjct: 81  NLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK--PTKRV---LKTSDRYQARVG 140

Query: 144 DQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQELVSCDT 203
           D LP+SVDWR  GAV  +KDQGSCGSCWAFSTI AVEGIN+IVTG LISLSEQELV CDT
Sbjct: 141 DALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSEQELVDCDT 200

Query: 204 DYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQCDPTRKNAKVVSIDGYEDVPVG 263
            YN GCNGGLMDYAF+FII NGG+DTE DYPY+  DG+CD  RKNAKVV+ID YEDVP  
Sbjct: 201 SYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTIDSYEDVPEN 260

Query: 264 DEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGTENGVDYWL 323
            E +LKKA+AHQP+SVAIEAGGRA QLY SGVF G CG++LDHGVVAVGYGTENG DYW+
Sbjct: 261 SEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYGTENGKDYWI 320

Query: 324 VRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTSDNP 354
           VRNSWG  WGE GY ++ RN++   +GKCGI ME+SYP+K   NP
Sbjct: 321 VRNSWGNRWGESGYIKMARNIE-APTGKCGIAMEASYPIKKGQNP 359

[Close]

BLAST of Moc01g25560 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 457.2 bits (1175), Expect = 1.2e-128
Identity = 226/366 (61.75%), Postives = 274/366 (74.86%), Query Frame = 0


Query: 4   ATSLAVLSFFFFFLLSALASASDMSVLSR---------RRDGEVREIYDLWLAKHGKAYN 63
           A S  VLS    +++ +LAS  +  +            R D EVR IY  W A+HGK  N
Sbjct: 2   APSTKVLSLLLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNN 61

Query: 64  G----IEEREKRFQIFKDNLNFIDEHNSENR--TYKVGLNRFADLSNDEYRAAYLGTRSN 123
                I +++KRF IFKDNL FID HN  N+  TYK+GL +F DL+NDEYR  YLG R+ 
Sbjct: 62  NNNGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTE 121

Query: 124 PVRRVMKAMKASRRYTVNA-GDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGI 183
           P RR+ KA   +++Y+    G ++PE+VDWR +GAV PIKDQG+CGSCWAFST AAVEGI
Sbjct: 122 PARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGI 181

Query: 184 NQIVTGKLISLSEQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLDGQC 243
           N+IVTG+LISLSEQELV CD  YN GCNGGLMDYAFQFI+ NGGL+TE+DYPY G  G+C
Sbjct: 182 NKIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKC 241

Query: 244 DPTRKNAKVVSIDGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGS 303
           +   KN++VVSIDGYEDVP  DE ALKKA+++QPVSVAIEAGGR  Q YQSG+FTG CG+
Sbjct: 242 NSFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGT 301

Query: 304 DLDHGVVAVGYGTENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPV 354
           +LDH VVAVGYG+ENGVDYW+VRNSWG  WGE+GY R+ERN+  + SGKCGI +E+SYPV
Sbjct: 302 NLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPV 361

[Close]

BLAST of Moc01g25560 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 445.7 bits (1145), Expect = 3.7e-125
Identity = 222/357 (62.18%), Postives = 274/357 (76.75%), Query Frame = 0


Query: 1   MATA-TSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEER 60
           MAT+  S+ +    F  LL +L+  S  +  + R + E R +Y+ WL ++ K YNG+ E+
Sbjct: 1   MATSIKSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEK 60

Query: 61  EKRFQIFKDNLNFIDEHNS-ENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMK 120
           E+RF+IFKDNL F++EH+S  NRTY+VGL RFADL+NDE+RA YL ++    R  +K  K
Sbjct: 61  ERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMERTRVPVKGEK 120

Query: 121 ASRRYTVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISL 180
               Y    GD LP+++DWRA+GAV P+KDQGSCGSCWAFS I AVEGINQI TG+LISL
Sbjct: 121 ----YLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISL 180

Query: 181 SEQELVSCDTDYNSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLD-GQCDPTRKNAKVV 240
           SEQELV CDT YN GC GGLMDYAF+FII+NGG+DTEEDYPY   D   C+  +KN +VV
Sbjct: 181 SEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVV 240

Query: 241 SIDGYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVG 300
           +IDGYEDVP  DE++LKKA+A+QP+SVAIEAGGRA QLY SGVFTG CG+ LDHGVVAVG
Sbjct: 241 TIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVG 300

Query: 301 YGTENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTS-DNP 354
           YG+E G DYW+VRNSWG+ WGE GYF+LERN+K  SSGKCG+ M +SYP K+S  NP
Sbjct: 301 YGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE-SSGKCGVAMMASYPTKSSGSNP 352

[Close]

BLAST of Moc01g25560 vs. TAIR 10
Match: AT3G19400.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 391.7 bits (1005), Expect = 6.4e-109
Identity = 198/350 (56.57%), Postives = 259/350 (74.00%), Query Frame = 0


Query: 5   TSLAVLSFFFFFLLSALASASDMSVLSRRRDGEVREIYDLWLAKHGKAYNGIEEREKRFQ 64
           ++L +LS     L S+L  A++  +   R + EVR +Y+ WL ++ K YNG+ E+E+RF+
Sbjct: 11  SALVILS--VLLLSSSLGVATETEI--ERNETEVRLMYEQWLVENRKNYNGLGEKERRFK 70

Query: 65  IFKDNLNFIDEHNS-ENRTYKVGLNRFADLSNDEYRAAYLGTRSNPVRRVMKAMKASRRY 124
           IFKDNL F+DEHNS  +RT++VGL RFADL+N+E+RA YL  +    +  +K    + RY
Sbjct: 71  IFKDNLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIYLRKKMERTKDSVK----TERY 130

Query: 125 TVNAGDQLPESVDWRARGAVGPIKDQGSCGSCWAFSTIAAVEGINQIVTGKLISLSEQEL 184
               GD LP+ VDWRA GAV  +KDQG+CGSCWAFS + AVEGINQI TG+LISLSEQEL
Sbjct: 131 LYKEGDVLPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQEL 190

Query: 185 VSCDTDY-NSGCNGGLMDYAFQFIIDNGGLDTEEDYPYEGLD-GQCDPTR-KNAKVVSID 244
           V CD  + N+GC+GG+M+YAF+FI+ NGG++T++DYPY   D G C+  +  N +VV+ID
Sbjct: 191 VDCDRGFVNAGCDGGIMNYAFEFIMKNGGIETDQDYPYNANDLGLCNADKNNNTRVVTID 250

Query: 245 GYEDVPVGDEEALKKAVAHQPVSVAIEAGGRALQLYQSGVFTGKCGSDLDHGVVAVGYGT 304
           GYEDVP  DE++LKKAVAHQPVSVAIEA  +A QLY+SGV TG CG  LDHGVV VGYG+
Sbjct: 251 GYEDVPRDDEKSLKKAVAHQPVSVAIEASSQAFQLYKSGVMTGTCGISLDHGVVVVGYGS 310

Query: 305 ENGVDYWLVRNSWGTEWGEDGYFRLERNVKNTSSGKCGITMESSYPVKTS 351
            +G DYW++RNSWG  WG+ GY +L+RN+ +   GKCGI M  SYP K+S
Sbjct: 311 TSGEDYWIIRNSWGLNWGDSGYVKLQRNI-DDPFGKCGIAMMPSYPTKSS 351

The following BLAST results are available for this feature:

BLAST of Moc01g25560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132954.1	1.9e-211	99.73	cysteine proteinase COT44 [Momordica charantia]	[more]
XP_023518142.1	2.0e-173	82.89	cysteine proteinase COT44-like [Cucurbita pepo subsp. pepo]	[more]
KAG7027046.1	7.7e-173	82.35	Oryzain alpha chain [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022963075.1	1.0e-172	82.35	zingipain-2-like [Cucurbita moschata]	[more]
XP_023544321.1	2.2e-172	82.97	cysteine proteinase COT44-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc01g25560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	9.9e-131	63.35	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	4.6e-128	64.64	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
Q94B08	1.0e-127	61.75	Germination-specific cysteine protease 1 OS=Arabidopsis thaliana OX=3702 GN=GCP1...	[more]
P25776	1.6e-125	63.56	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
Q9LT78	5.3e-124	62.18	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]

BLAST of Moc01g25560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUJ2	9.1e-212	99.73	cysteine proteinase COT44 OS=Momordica charantia OX=3673 GN=LOC111005671 PE=3 SV...	[more]
A0A6J1HGZ6	4.9e-173	82.35	zingipain-2-like OS=Cucurbita moschata OX=3662 GN=LOC111463384 PE=3 SV=1	[more]
A0A6J1KPD5	1.4e-172	82.62	cysteine proteinase COT44-like OS=Cucurbita maxima OX=3661 GN=LOC111497083 PE=3 ...	[more]
A0A5D3CLQ0	4.1e-172	83.38	Cysteine proteinase COT44-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B0U6	4.1e-172	83.38	cysteine proteinase COT44-like OS=Cucumis melo OX=3656 GN=LOC103484794 PE=3 SV=1	[more]

BLAST of Moc01g25560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	7.1e-132	63.35	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	3.3e-129	64.64	Granulin repeat cysteine protease family protein	[more]
AT4G36880.1	1.2e-128	61.75	cysteine proteinase1	[more]
AT3G19390.1	3.7e-125	62.18	Granulin repeat cysteine protease family protein	[more]
AT3G19400.1	6.4e-109	56.57	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 291..301 score: 58.59 coord: 149..164 score: 65.92 coord: 306..312 score: 69.74
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 131..347 e-value: 1.7E-127 score: 439.5
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 131..347 e-value: 1.2E-83 score: 280.6
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 42..98 e-value: 4.9E-26 score: 102.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 42..98 e-value: 5.9E-18 score: 65.1
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 25..349 e-value: 3.0E-122 score: 410.1
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 26..356
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 26..356
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 149..160
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 306..325
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 289..299
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 132..346 e-value: 2.48828E-112 score: 324.577
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 35..347

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g25560.1	Moc01g25560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity