Moc01g22560 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g22560
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionE3 ubiquitin-protein ligase RZFP34-like
Locationchr1: 15671773 .. 15673846 (-)
RNA-Seq ExpressionMoc01g22560
SyntenyMoc01g22560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCTCGGTTGTCTACGAATGGGACCTTCTGTAAGGGCGATAAATCTTCGATCGATATCGGATATGGCAGCAACGGGTAATTAATCTTTCTCATGCAGCGTTTACATAAATTTTCCATCTGATTTGTGCGGTTTTTGGTAGCTGCGGGATATTACTGAGCTTTTTTTCCCCTGTGAAATTTTTCTGAAGGTGTTCCCATTATAGAAGGAGGTGTAAGATCAGAGCACCTTGTTGCAATGAGATATTTGATTGCAGGCATTGCCATAACGAAGCTAAGGTTGGCTGCTGCTTTGTTTTATTGTTTCTATTTTCCTCTCTCTGTTTCATATCTATAAAATTGTGTGAGAGATGGATTGATGAAGCTTATTTCAATGGTCTTTCTGTAGAACTCGTTAGATGTGGATCCTCTCTTACGCCACGACATTCCTCGCCATGATATGAAAGCAGTAAGTTTCCTTGTTTTTCTTTGTAGCTACACTCTTTGAATTGTTTTTCCTCTCTCTGTTTCATATCTATAAAATTGTTACTGATTATTGGTATTGAGATTTCTGGAAACTATATATATATATTTTTTTGGTCACTTCCTTAAGTTCAATTTGGCTAGGATCTTTTGTGTTTAGGAGATTACAAACAAAGATATTGAGAGTAAAGTAAGCTTATTTTTATTAAAAAAAAATAGAATTCTTATCAAGAAGACCTTTAAATTCTCTATATTATGAATTCTAATTCGTTAATTCTTGTGTTGAATTTCCCTACAGGTCATCTGTTCTTTGTGTGGCACGGAACAGGATGTAAGTCCTTTCTTTAGAAACTCCTTCACTGAATTTTGGGAGATAGAATTAAATACCTTTGGTTACTTCTTCTAGGTGCGACAACATTGTATTCAATGTGGAGTTTGTATGGGGAAGTACTTTTGCGCAAAGTGCAAGTTCTTCGATGACGATGTAAGCTTCTATCGGCTAGTTAACTTTCGACATTCTTAGTCGTGTTCTTAATTTTTTGGTAACTCAAAATCTTATTTTATTTGCTAGGTTTCAAAGAAGCAGTACCATTGTAATGAATGTGGCATTTGCAGGTCCAACCATTTCCTGTATCTTCTCTGTACTTTTATGTAGTTACCTTTCTTACTCCACTGCCCTCATTTGATTTATTAGATGCATTTTTAGTACACAGTTATATTTTCTTTTTTCGTTACGCAGAGTGGGAGGTGAGGAGAATTTTTTCCATTGCAACAAGTGTGGTAGGTTTTTTTGGACATTTTTTATGAGTCAACTGGTTGGAGTTCTACTTTTTCAGCCATTGAATTTTGGAGATGTTCCTTCATCCTTTCAGGTTGTTGCTATTCAAAACTGATGGAGAACTCCCACAATTGTGTGGAGAAGGCAATGCACCACAACTGTCCTGTTTGTTTTGAGGTGAATTTCAAAACATGGAATTCTGATGTTCATATACTTAGCCATATATTTATTAAATGTGCCATGTCAATAATACAATCAACTGTGTCGCTGCAGTTTCTTTTCGATACAATGAGAGACATTTCCGTTCTCCCATGTGGACACACTATACATTTAGAATGTGTGAAGGAAATGGAGCAACGCTTACAGTAAGTCCAGTTTCTGTGTTACAGAAAATGCTTTTTGTTCTGATCATTTGCTCTGTGTCTTTACTGGTTGGGTTAAATCTGCAGATATACATGTCCTGTTTGCTCAAAATCCTACTGCGACATGTCGAGCGTGTGGGAGAAACTGGATCACGAGGTCGCATCGACGCCCATGCCCCAAATATATCAACATAAAATGGTTAGTTTAGTAGTGGGTGCAAAACAATTTGTGGCTTCTCAAAAGAAACAGCTGATTAATTTGATAAACTGACAAAGTTGCTTCTTTGACTGTTGGTTAGGTTTGGATCTTGTGCAATGACTGGATCTTGTGCAATGACTGTAGTGAGATATCAGAGGTAAATTATCACATTGTGGCACACAAGTGCCTCAACTGCAAGTCCTATAATACAAGACTGACCCAAGGTGGGCCCTCTTCATGCTCTCCAAGGATTGCTCAAATGGTGAGATGA

mRNA sequence

ATGGAGTCTCGGTTGTCTACGAATGGGACCTTCTGTAAGGGCGATAAATCTTCGATCGATATCGGATATGGCAGCAACGGGTGTTCCCATTATAGAAGGAGGTGTAAGATCAGAGCACCTTGTTGCAATGAGATATTTGATTGCAGGCATTGCCATAACGAAGCTAAGAACTCGTTAGATGTGGATCCTCTCTTACGCCACGACATTCCTCGCCATGATATGAAAGCAGTCATCTGTTCTTTGTGTGGCACGGAACAGGATGTGCGACAACATTGTATTCAATGTGGAGTTTGTATGGGGAAGTACTTTTGCGCAAAGTGCAAGTTCTTCGATGACGATGTTTCAAAGAAGCAGTACCATTGTAATGAATGTGGCATTTGCAGAGTGGGAGGTGAGGAGAATTTTTTCCATTGCAACAAGTGTGGTTGTTGCTATTCAAAACTGATGGAGAACTCCCACAATTGTGTGGAGAAGGCAATGCACCACAACTGTCCTGTTTGTTTTGAGTTTCTTTTCGATACAATGAGAGACATTTCCGTTCTCCCATGTGGACACACTATACATTTAGAATGTGTGAAGGAAATGGAGCAACGCTTACAATATACATGTCCTGTTTGCTCAAAATCCTACTGCGACATGTCGAGCGTGTGGGAGAAACTGGATCACGAGGTCGCATCGACGCCCATGCCCCAAATATATCAACATAAAATGGTTTGGATCTTGTGCAATGACTGGATCTTGTGCAATGACTGTAGTGAGATATCAGAGGTAAATTATCACATTGTGGCACACAAGTGCCTCAACTGCAAGTCCTATAATACAAGACTGACCCAAGGTGGGCCCTCTTCATGCTCTCCAAGGATTGCTCAAATGGTGAGATGA

Coding sequence (CDS)

ATGGAGTCTCGGTTGTCTACGAATGGGACCTTCTGTAAGGGCGATAAATCTTCGATCGATATCGGATATGGCAGCAACGGGTGTTCCCATTATAGAAGGAGGTGTAAGATCAGAGCACCTTGTTGCAATGAGATATTTGATTGCAGGCATTGCCATAACGAAGCTAAGAACTCGTTAGATGTGGATCCTCTCTTACGCCACGACATTCCTCGCCATGATATGAAAGCAGTCATCTGTTCTTTGTGTGGCACGGAACAGGATGTGCGACAACATTGTATTCAATGTGGAGTTTGTATGGGGAAGTACTTTTGCGCAAAGTGCAAGTTCTTCGATGACGATGTTTCAAAGAAGCAGTACCATTGTAATGAATGTGGCATTTGCAGAGTGGGAGGTGAGGAGAATTTTTTCCATTGCAACAAGTGTGGTTGTTGCTATTCAAAACTGATGGAGAACTCCCACAATTGTGTGGAGAAGGCAATGCACCACAACTGTCCTGTTTGTTTTGAGTTTCTTTTCGATACAATGAGAGACATTTCCGTTCTCCCATGTGGACACACTATACATTTAGAATGTGTGAAGGAAATGGAGCAACGCTTACAATATACATGTCCTGTTTGCTCAAAATCCTACTGCGACATGTCGAGCGTGTGGGAGAAACTGGATCACGAGGTCGCATCGACGCCCATGCCCCAAATATATCAACATAAAATGGTTTGGATCTTGTGCAATGACTGGATCTTGTGCAATGACTGTAGTGAGATATCAGAGGTAAATTATCACATTGTGGCACACAAGTGCCTCAACTGCAAGTCCTATAATACAAGACTGACCCAAGGTGGGCCCTCTTCATGCTCTCCAAGGATTGCTCAAATGGTGAGATGA

Protein sequence

MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR
Homology
BLAST of Moc01g22560 vs. NCBI nr
Match: XP_022143702.1 (E3 ubiquitin-protein ligase RZFP34-like isoform X3 [Momordica charantia])

HSP 1 Score: 656.8 bits (1693), Expect = 9.1e-185
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180
           CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV
Sbjct: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180

Query: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240
           LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI
Sbjct: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240

Query: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 294
           LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR
Sbjct: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 293

BLAST of Moc01g22560 vs. NCBI nr
Match: XP_022143700.1 (E3 ubiquitin-protein ligase RZFP34-like isoform X1 [Momordica charantia])

HSP 1 Score: 647.9 bits (1670), Expect = 4.2e-182
Identity = 293/305 (96.07%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 121 CNECGICR------------VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180
           CNECGICR            VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF
Sbjct: 121 CNECGICRSNHFLYLLCTFIVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180

Query: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240
           EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP
Sbjct: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240

Query: 241 MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 294
           MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI
Sbjct: 241 MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 300

BLAST of Moc01g22560 vs. NCBI nr
Match: XP_022143701.1 (E3 ubiquitin-protein ligase RZFP34-like isoform X2 [Momordica charantia])

HSP 1 Score: 625.9 bits (1613), Expect = 1.7e-175
Identity = 287/305 (94.10%), Postives = 287/305 (94.10%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 121 CNECGICR------------VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180
           CNECGICR            VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF
Sbjct: 121 CNECGICRSNHFLYLLCTFIVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180

Query: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240
           EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP
Sbjct: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240

Query: 241 MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 294
           MPQIYQHKMV      WILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI
Sbjct: 241 MPQIYQHKMV------WILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 299

BLAST of Moc01g22560 vs. NCBI nr
Match: XP_004143281.1 (E3 ubiquitin-protein ligase RZFP34 isoform X1 [Cucumis sativus] >XP_011657675.1 E3 ubiquitin-protein ligase RZFP34 isoform X1 [Cucumis sativus] >KGN48202.1 hypothetical protein Csa_003793 [Cucumis sativus])

HSP 1 Score: 583.2 bits (1502), Expect = 1.3e-162
Identity = 265/293 (90.44%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           ME+ +S N     GD SSID+GYGS GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 7   MEAWISPN-----GDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 66

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPR DM+ VICSLCGTEQDVRQ+CIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 67  VDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 126

Query: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180
           CNECGICRVGGE+NFFHC KCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTM DISV
Sbjct: 127 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 186

Query: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240
           LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQ+YQHKMV  
Sbjct: 187 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQMYQHKMV-- 246

Query: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 294
               WILCNDCSEISEVNYHIVAHKCL CKSYNTRLTQGGPSSCSPRIAQ+VR
Sbjct: 247 ----WILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPSSCSPRIAQIVR 288

BLAST of Moc01g22560 vs. NCBI nr
Match: KAA0063794.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 582.8 bits (1501), Expect = 1.7e-162
Identity = 265/293 (90.44%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           ME+ +S N     GD SSID+GYGS GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 6   MEAWISPN-----GDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 65

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDM+ VICSLCGTEQDVRQ+CIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 66  VDPLLRHDIPRHDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 125

Query: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180
           CNECGICRVGGE+NFFHC KCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTM DISV
Sbjct: 126 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 185

Query: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240
           LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQ+YQ KMV  
Sbjct: 186 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQMYQDKMV-- 245

Query: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 294
               WILCNDCSEISEVNYHIVAHKCL CKSYNTRLTQGGPSSCSPRIAQ+VR
Sbjct: 246 ----WILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPSSCSPRIAQIVR 287

BLAST of Moc01g22560 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 9.6e-121
Identity = 187/276 (67.75%), Postives = 226/276 (81.88%), Query Frame = 0

Query: 19  IDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVI 78
           ++IG G  GCSHYRRRCKIRAPCC+EIFDCRHCHNEAK+SL ++   RH++PRH++  VI
Sbjct: 18  MEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVI 77

Query: 79  CSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHC 138
           CSLC TEQDV+Q+C  CGVCMGKYFC+KCKFFDDD+SKKQYHC+ECGICR GGEENFFHC
Sbjct: 78  CSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHC 137

Query: 139 NKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQR 198
            +C CCYSK+ME+ H CVE AMHHNCPVCFE+LFD+ RDI+VL CGHT+HLEC K+M   
Sbjct: 138 KRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLH 197

Query: 199 LQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVN 258
            +YTCPVCSKS CDMS++W+KLD EVA+ PMP++Y++KMV      WILCNDC   + V 
Sbjct: 198 NRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMV------WILCNDCGSNTNVR 257

Query: 259 YHIVAHKCLNCKSYNTRLTQGGPS--SCSPRIAQMV 293
           +H++AHKC +C SYNTR TQ G    SCS  + Q+V
Sbjct: 258 FHLIAHKCSSCGSYNTRQTQRGSDSHSCSSGMPQVV 287

BLAST of Moc01g22560 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 1.8e-111
Identity = 169/261 (64.75%), Postives = 210/261 (80.46%), Query Frame = 0

Query: 15  DKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDM 74
           D   ++ G    GC+HYRRRC+IRAPCCNEIFDCRHCHNE KNS+ +D + RH++PRH++
Sbjct: 48  DYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVKRHELPRHEV 107

Query: 75  KAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEEN 134
           + VICSLCGTEQ+VRQ CI CGVCMGKYFC  CK FDDDVSK+QYHCN CGICR+GG+EN
Sbjct: 108 QQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCGICRIGGKEN 167

Query: 135 FFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKE 194
           FFHC+KCGCCYS +++NSH CVE AMHH+CP+CFE+LF++  D+SVLPCGHTIH++C++E
Sbjct: 168 FFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGHTIHVKCLRE 227

Query: 195 MEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEI 254
           ME+  Q+ CP+CSKS CDMS  WE+LD E+A+  +     +KMV       ILCNDC   
Sbjct: 228 MEEHCQFACPLCSKSVCDMSKAWERLDEELAT--ISDTCDNKMV------RILCNDCGAT 287

Query: 255 SEVNYHIVAHKCLNCKSYNTR 276
           SEV +H++AHKC  CKSYNTR
Sbjct: 288 SEVQFHLIAHKCQKCKSYNTR 300

BLAST of Moc01g22560 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 2.9e-93
Identity = 140/249 (56.22%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 27  GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVICSLCGTEQ 86
           GC HY+RRC+IRAPCCNE+FDCRHCHNE+ ++L  +   RHD+ R D+K VICS+C TEQ
Sbjct: 17  GCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLR-NIYDRHDLVRQDVKQVICSVCDTEQ 76

Query: 87  DVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHCNKCGCCYS 146
              Q C  CGV MG+YFC+ C F+DDD  K+Q+HC++CGICRVGG ENFFHC KCG CY+
Sbjct: 77  PAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHCKKCGSCYA 136

Query: 147 KLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVC 206
             + N+H CVE +M H+CP+C+E+LFD+++D +V+ CGHT+H+EC  EM +R ++ CP+C
Sbjct: 137 VGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKRDKFCCPIC 196

Query: 207 SKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKC 266
           S+S  DMS  W++LD E+ +T MP  Y+ K V      WILCNDC++ +EV++HI+  KC
Sbjct: 197 SRSVIDMSKTWQRLDEEIEATAMPSDYRDKKV------WILCNDCNDTTEVHFHIIGQKC 256

Query: 267 LNCKSYNTR 276
            +C+SYNTR
Sbjct: 257 GHCRSYNTR 258

BLAST of Moc01g22560 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.5e-81
Identity = 127/260 (48.85%), Postives = 174/260 (66.92%), Query Frame = 0

Query: 15  DKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDM 74
           D S    G    GC HYRRRC+IRAPCCN++F CRHCHNE+           H++ RH +
Sbjct: 5   DASRHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNESTKD-------GHELDRHAV 64

Query: 75  KAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEEN 134
           ++VIC +C TEQ V Q C  CGVCMG+YFC+ CKFFDDDV ++ +HC +CGICRVGG++N
Sbjct: 65  ESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGKDN 124

Query: 135 FFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKE 194
           FFHC KCG CYS  + + H C+E +M +NCP+C+E+LFD++R+ SVL CGHT+HL+C  E
Sbjct: 125 FFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCFHE 184

Query: 195 MEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEI 254
           M +  +++CP+CS    DM      LD E+ +  +   Y  K        WI+CNDC + 
Sbjct: 185 MLKHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMGK-------GWIVCNDCRDT 244

Query: 255 SEVNYHIVAHKCLNCKSYNT 275
           ++V   +  HKC +C+S+NT
Sbjct: 245 TQVYARVAGHKCCHCQSHNT 250

BLAST of Moc01g22560 vs. ExPASy Swiss-Prot
Match: Q96PM5 (RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=RCHY1 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 9.4e-60
Identity = 108/256 (42.19%), Postives = 144/256 (56.25%), Query Frame = 0

Query: 24  GSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVICSLCG 83
           G  GC HY R C ++APCC++++ CR CH+  ++         H + R  +K V C  C 
Sbjct: 16  GQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNED---------HQLDRFKVKEVQCINCE 75

Query: 84  TEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHCNKCGC 143
             Q  +Q C +C    G+Y+C  C  FD D  KKQYHC  CGICR+G +E+FFHC KC  
Sbjct: 76  KIQHAQQTCEECSTLFGEYYCDICHLFDKD--KKQYHCENCGICRIGPKEDFFHCLKCNL 135

Query: 144 CYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTC 203
           C +  ++  H C+E     NCP+C E +  +     VLPCGH +H  C +EM +   Y C
Sbjct: 136 CLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKE-GYRC 195

Query: 204 PVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVA 263
           P+C  S  DM+  W +LD EVA TPMP  YQ+  V       ILCNDC+  S V +HI+ 
Sbjct: 196 PLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTV------DILCNDCNGRSTVQFHILG 253

Query: 264 HKCLNCKSYNTRLTQG 280
            KC  C+SYNT    G
Sbjct: 256 MKCKICESYNTAQAGG 253

BLAST of Moc01g22560 vs. ExPASy TrEMBL
Match: A0A6J1CRL3 (E3 ubiquitin-protein ligase RZFP34-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111013545 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 4.4e-185
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180
           CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV
Sbjct: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180

Query: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240
           LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI
Sbjct: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240

Query: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 294
           LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR
Sbjct: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 293

BLAST of Moc01g22560 vs. ExPASy TrEMBL
Match: A0A6J1CPI2 (E3 ubiquitin-protein ligase RZFP34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013545 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.0e-182
Identity = 293/305 (96.07%), Postives = 293/305 (96.07%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 121 CNECGICR------------VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180
           CNECGICR            VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF
Sbjct: 121 CNECGICRSNHFLYLLCTFIVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180

Query: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240
           EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP
Sbjct: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240

Query: 241 MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 294
           MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI
Sbjct: 241 MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 300

BLAST of Moc01g22560 vs. ExPASy TrEMBL
Match: A0A6J1CR32 (E3 ubiquitin-protein ligase RZFP34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013545 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 8.3e-176
Identity = 287/305 (94.10%), Postives = 287/305 (94.10%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 121 CNECGICR------------VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180
           CNECGICR            VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF
Sbjct: 121 CNECGICRSNHFLYLLCTFIVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCF 180

Query: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240
           EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP
Sbjct: 181 EFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTP 240

Query: 241 MPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 294
           MPQIYQHKMV      WILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI
Sbjct: 241 MPQIYQHKMV------WILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRI 299

BLAST of Moc01g22560 vs. ExPASy TrEMBL
Match: A0A0A0KIG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448100 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.2e-163
Identity = 265/293 (90.44%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           ME+ +S N     GD SSID+GYGS GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 7   MEAWISPN-----GDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 66

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPR DM+ VICSLCGTEQDVRQ+CIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 67  VDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 126

Query: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180
           CNECGICRVGGE+NFFHC KCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTM DISV
Sbjct: 127 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 186

Query: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240
           LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQ+YQHKMV  
Sbjct: 187 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQMYQHKMV-- 246

Query: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 294
               WILCNDCSEISEVNYHIVAHKCL CKSYNTRLTQGGPSSCSPRIAQ+VR
Sbjct: 247 ----WILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPSSCSPRIAQIVR 288

BLAST of Moc01g22560 vs. ExPASy TrEMBL
Match: A0A5A7V9A0 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00080 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.0e-163
Identity = 265/293 (90.44%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MESRLSTNGTFCKGDKSSIDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60
           ME+ +S N     GD SSID+GYGS GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 6   MEAWISPN-----GDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 65

Query: 61  VDPLLRHDIPRHDMKAVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120
           VDPLLRHDIPRHDM+ VICSLCGTEQDVRQ+CIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 66  VDPLLRHDIPRHDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 125

Query: 121 CNECGICRVGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISV 180
           CNECGICRVGGE+NFFHC KCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTM DISV
Sbjct: 126 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 185

Query: 181 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWI 240
           LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQ+YQ KMV  
Sbjct: 186 LPCGHTIHLECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQMYQDKMV-- 245

Query: 241 LCNDWILCNDCSEISEVNYHIVAHKCLNCKSYNTRLTQGGPSSCSPRIAQMVR 294
               WILCNDCSEISEVNYHIVAHKCL CKSYNTRLTQGGPSSCSPRIAQ+VR
Sbjct: 246 ----WILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPSSCSPRIAQIVR 287

BLAST of Moc01g22560 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 434.5 bits (1116), Expect = 6.8e-122
Identity = 187/276 (67.75%), Postives = 226/276 (81.88%), Query Frame = 0

Query: 19  IDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVI 78
           ++IG G  GCSHYRRRCKIRAPCC+EIFDCRHCHNEAK+SL ++   RH++PRH++  VI
Sbjct: 18  MEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVI 77

Query: 79  CSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHC 138
           CSLC TEQDV+Q+C  CGVCMGKYFC+KCKFFDDD+SKKQYHC+ECGICR GGEENFFHC
Sbjct: 78  CSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHC 137

Query: 139 NKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQR 198
            +C CCYSK+ME+ H CVE AMHHNCPVCFE+LFD+ RDI+VL CGHT+HLEC K+M   
Sbjct: 138 KRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLH 197

Query: 199 LQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVN 258
            +YTCPVCSKS CDMS++W+KLD EVA+ PMP++Y++KMV      WILCNDC   + V 
Sbjct: 198 NRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMV------WILCNDCGSNTNVR 257

Query: 259 YHIVAHKCLNCKSYNTRLTQGGPS--SCSPRIAQMV 293
           +H++AHKC +C SYNTR TQ G    SCS  + Q+V
Sbjct: 258 FHLIAHKCSSCGSYNTRQTQRGSDSHSCSSGMPQVV 287

BLAST of Moc01g22560 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 406.8 bits (1044), Expect = 1.5e-113
Identity = 174/261 (66.67%), Postives = 205/261 (78.54%), Query Frame = 0

Query: 19  IDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVI 78
           +D G    GC HYRRRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPRH ++ VI
Sbjct: 54  LDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVI 113

Query: 79  CSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHC 138
           C LCGTEQ+V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR+GG ENFFHC
Sbjct: 114 CLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHC 173

Query: 139 NKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQR 198
            KCGCCYS L++N H CVE AMHH+CP+CFEFLF++  D++VLPCGHTIH +C++EM   
Sbjct: 174 YKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDH 233

Query: 199 LQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVN 258
            QY CP+CSKS CDMS VWEK D E+A+TPMP+ YQ++MV       ILCNDC + +EV 
Sbjct: 234 YQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMV------QILCNDCGKKAEVQ 293

Query: 259 YHIVAHKCLNCKSYNTRLTQG 280
           YH+VA KC NCKSYNTR T+G
Sbjct: 294 YHVVAQKCPNCKSYNTRQTRG 308

BLAST of Moc01g22560 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 398.7 bits (1023), Expect = 4.1e-111
Identity = 174/271 (64.21%), Postives = 205/271 (75.65%), Query Frame = 0

Query: 19  IDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMK--- 78
           +D G    GC HYRRRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPRH ++   
Sbjct: 54  LDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQLT 113

Query: 79  -------AVICSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICR 138
                   VIC LCGTEQ+V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR
Sbjct: 114 RPLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICR 173

Query: 139 VGGEENFFHCNKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIH 198
           +GG ENFFHC KCGCCYS L++N H CVE AMHH+CP+CFEFLF++  D++VLPCGHTIH
Sbjct: 174 IGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIH 233

Query: 199 LECVKEMEQRLQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILC 258
            +C++EM    QY CP+CSKS CDMS VWEK D E+A+TPMP+ YQ++MV       ILC
Sbjct: 234 QKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMV------QILC 293

Query: 259 NDCSEISEVNYHIVAHKCLNCKSYNTRLTQG 280
           NDC + +EV YH+VA KC NCKSYNTR T+G
Sbjct: 294 NDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 318

BLAST of Moc01g22560 vs. TAIR 10
Match: AT5G25560.4 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 395.2 bits (1014), Expect = 4.6e-110
Identity = 169/253 (66.80%), Postives = 199/253 (78.66%), Query Frame = 0

Query: 27  GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVICSLCGTEQ 86
           G   Y RRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPRH ++ VIC LCGTEQ
Sbjct: 57  GLMEYGRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVICLLCGTEQ 116

Query: 87  DVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHCNKCGCCYS 146
           +V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR+GG ENFFHC KCGCCYS
Sbjct: 117 EVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHCYKCGCCYS 176

Query: 147 KLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQRLQYTCPVC 206
            L++N H CVE AMHH+CP+CFEFLF++  D++VLPCGHTIH +C++EM    QY CP+C
Sbjct: 177 ILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDHYQYACPLC 236

Query: 207 SKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDWILCNDCSEISEVNYHIVAHKC 266
           SKS CDMS VWEK D E+A+TPMP+ YQ++MV       ILCNDC + +EV YH+VA KC
Sbjct: 237 SKSVCDMSKVWEKFDMEIAATPMPEPYQNRMV------QILCNDCGKKAEVQYHVVAQKC 296

Query: 267 LNCKSYNTRLTQG 280
            NCKSYNTR T+G
Sbjct: 297 PNCKSYNTRQTRG 303

BLAST of Moc01g22560 vs. TAIR 10
Match: AT5G25560.2 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 360.9 bits (925), Expect = 9.6e-100
Identity = 152/227 (66.96%), Postives = 179/227 (78.85%), Query Frame = 0

Query: 19  IDIGYGSNGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRHDMKAVI 78
           +D G    GC HYRRRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPRH ++ VI
Sbjct: 54  LDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVI 113

Query: 79  CSLCGTEQDVRQHCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEENFFHC 138
           C LCGTEQ+V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR+GG ENFFHC
Sbjct: 114 CLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHC 173

Query: 139 NKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMRDISVLPCGHTIHLECVKEMEQR 198
            KCGCCYS L++N H CVE AMHH+CP+CFEFLF++  D++VLPCGHTIH +C++EM   
Sbjct: 174 YKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDH 233

Query: 199 LQYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDW 246
            QY CP+CSKS CDMS VWEK D E+A+TPMP+ YQ++MV  L   W
Sbjct: 234 YQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVNSLQRLW 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143702.19.1e-185100.00E3 ubiquitin-protein ligase RZFP34-like isoform X3 [Momordica charantia][more]
XP_022143700.14.2e-18296.07E3 ubiquitin-protein ligase RZFP34-like isoform X1 [Momordica charantia][more]
XP_022143701.11.7e-17594.10E3 ubiquitin-protein ligase RZFP34-like isoform X2 [Momordica charantia][more]
XP_004143281.11.3e-16290.44E3 ubiquitin-protein ligase RZFP34 isoform X1 [Cucumis sativus] >XP_011657675.1 ... [more]
KAA0063794.11.7e-16290.44E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9FFB69.6e-12167.75E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q5JL961.8e-11164.75Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q8VZK02.9e-9356.22E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q10LI11.5e-8148.85E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q96PM59.4e-6042.19RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9... [more]
Match NameE-valueIdentityDescription
A0A6J1CRL34.4e-185100.00E3 ubiquitin-protein ligase RZFP34-like isoform X3 OS=Momordica charantia OX=367... [more]
A0A6J1CPI22.0e-18296.07E3 ubiquitin-protein ligase RZFP34-like isoform X1 OS=Momordica charantia OX=367... [more]
A0A6J1CR328.3e-17694.10E3 ubiquitin-protein ligase RZFP34-like isoform X2 OS=Momordica charantia OX=367... [more]
A0A0A0KIG36.2e-16390.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448100 PE=4 SV=1[more]
A0A5A7V9A08.0e-16390.44E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT5G22920.16.8e-12267.75CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.11.5e-11366.67CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.34.1e-11164.21CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.44.6e-11066.80CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.29.6e-10066.96CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 164..206
e-value: 3.8E-7
score: 39.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 164..207
score: 12.194218
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 157..209
e-value: 2.0E-10
score: 42.0
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 28..109
e-value: 4.4E-18
score: 65.5
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 21..97
score: 23.995043
NoneNo IPR availableGENE3D2.20.28.10coord: 245..282
e-value: 1.8E-16
score: 61.1
NoneNo IPR availablePANTHERPTHR21319:SF54E3 UBIQUITIN-PROTEIN LIGASE RZFP34coord: 245..292
NoneNo IPR availablePANTHERPTHR21319:SF54E3 UBIQUITIN-PROTEIN LIGASE RZFP34coord: 1..244
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 1..244
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 245..292
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 162..212
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 164..204
e-value: 8.7E-8
score: 32.1
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 245..275
e-value: 1.4E-12
score: 47.4
coord: 211..244
e-value: 5.2E-10
score: 39.1
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 99..163
score: 34.051598
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 100..157
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 26..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g22560.1Moc01g22560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding