Moc01g20460 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g20460
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionLaccase
Locationchr1: 14313453 .. 14316691 (-)
RNA-Seq ExpressionMoc01g20460
SyntenyMoc01g20460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCGCTGCCATTCTCCAGCATTCTTTCACTGTAAAATTCTCCATCTTCATGCATATGCCTCATTATCATCATTCGTATGTTATATGGTTTTTGGAGAAAAATAAAATGCCTGTATAAAGTATAATTTTCTTTTTTTTTTTTTCTCTTTTCTTTTCTTCTTGAAAGTCGAACTTGTTCACCTTCTTTTGAAATTGTTTTCAAAATATTTTTTTACTCATAGAATAATATTATTGTTCTAATTTGTAGTTTTCTTTGAATGAATGAATCCTGCTAAAAAGTTGTCAAACTTGAACCTTATTTGAATTAGGTTCAAGTTCGGGCAACTTTTTATGATAAAACTTCAAATTCTTAAAATAATTTTGATAGGTATTTTAAAAAATATTGCAATTTTTCCTCTCTTTTTTTGTTAATATAGCATAAATTTGACTAATTTTAGTGTGTACCTTTTTTATTACATATTTGAGTTTGATGATTTGAATTATGTCCTTTTTGAAAATTTTATATTTCTGGCTATATTTGAAAATAGATATTTATGTTATCTTATTTATGTAAATGCTTCAATAAATTTTAGAAAATTGTCTGTGAAAATACATTTAGATAACTTTCTTTAAATAAATTTTCTTATTTTCCATATTTTCTTTGATTGTATTGACAAATGAACTTACGTAAAATGATTTTTAACTCTTCCAATAACATTAACTATTATTTCAATAACAAAAATTTAAACACAGTTTTGAAAACAAAACGTAAAACTATTTGTCCAAACAGCCTCTTGATATACATAAAAAATTCTATGATTTCTTTTGCATGCATATCAGAAGTGAATATTATCGAGATGAATATTTCAAAATATATATATATATATTTTTTTTTTTTAACTAATGCTTATTCTCTCTCTACTTGTATAGGTGCAAGACATGACCGTCCGCCGGCTCTGCCGTGACCAAGTTATAACGGCCGTCAACGGAAATTCCCGGGTCCAACCATTCACGCTCAAGACGGCGACGTTCTGGTCGTCCACGTCTTCAACAAGTCCCCTTACGATTTAACCATCCACTGGTAAATTATTCTAAATTAATTTAGTTTGTTTGATTAGAAATTCTGAATCTATTTTTTGTTTTCATCAAATTGATTGATCTCGTTGAACAAAATTTGGAAAATGCGTTTTTTTAAATTGTTTTCGAATTTTGTTATCCAAATTATCAAAATTGACACGATGTCAATTACCGCTGAACTATTGCTCGCTTTAACAAAATATAATCGTATTCTAAATTATGTAAAATTCACAAAATATATTAATTAATTAATTAATAAAGTTTAGATTAAACTATATTTAATGGTAACTATTTCTAGCTTATAAATATATTATTAGAACAATTTTTTAAATTAATACCAACCAAAACCACCTTTGATTATTCGTTGATGTCTGATCATTGCCATATTTTTTTCTTTTAACTATTCCGATGATCTGTAATTGGTCAGGCATGGGGTTTTCCAGCTGCTCAGCGCGTGGGCCGACGGGCCGGAGAACGTAACGCAATGTCCGATACGGTCCGGCGAAAACTACACGTACAGATTCAAGATCACCGGACAAGAGGGGACTCTGTGGTGGCACGCGCACTCGTCTTGGCTACGCGCCACCGTCCACGGCGCCCTCCTCATCGCCCCCAAGTCCGGCCGACCGTTGCCCTTCCCGAAACCTTACAGGCAGATTCCGATCTTGCTCGGAGAGTGGTGGAACGCCAACGTCGTTGAGGTTGAAGAGGGAGGTCTCGCTACCGGCGCCGGTCCGAATAACTCCGACGCCTACACCATTAATGCCCTCCCCGGAAATCTCTACCCTTGCTCGCAAAATCGTAAGTAAAATATTCCACCCATACAAATTTTAGGCGCATGCATAATTTCCACTATTCAAAAAAATTTATAAAATCTTATTTTTCCACTGTAGGTTTAGTTTCCTTGTAAATTGTAATCAAAACTTTAATTTTCAATTCGTCCTGCAAATCTTAAAGTTTTAATTTAGTAGTGATGTGACCTCAACAAAAATTAATAATAATAGTAGTGATGCTTCTTATTTTAAAAATTTTAATTTGTTGGTGGTGATTTAAATTTATTGATTATTTTCAAACTGTCTTTTATATCCTCAGAAACCTATCTTCCTTTTTCATAATTTCTAAAGTGTATCAATACTGCAACAAATAAGGTTTTTTCTAATGCATCGGCAAAACCCTTAGAAACTTCGGGAGTCACATTTAGTTGGACAAAATGCAGGAAAAGATACTCCCAACGTTTTTTTTAGAAAATATTGGAAAAATATCACTTTTCCTCGTCTTCTTTTTTAAAATAATTGAAAAAAAAATATTTATTTTCCAAACGGTTTGTGAAAATGTGATTTGACTTAGTTTTAAAAGAATTTGATTGCCATTTTTTTAATTTTTATTTGATCCATACAGAGTTTTAAACTTTCATTTTTATCTTAATAATTTGGTCAAACTCTTACTAATTAAATATACTCCAAATTGTCATCTACCCATCCAATAAAAAAAAATTGTCAAGATATTCATATGATATAGAACATAAATTATAAAGATCATAAAGTTGAAATTTTTAAAATTATTGAAACAAATTAAATTGAAATTAAGCTTAAATAATATTGAAACTTTTAGAAGTGGAGACGCTAAATTGAAAGACTAAATTGTCAAATATATATATATATATATATATGTAATCCCATTAAACCAGTTTTTCGATTCCTATTTTTAGAAACCTATCAACTGAAGATGGTGCCCAAAAAGACATACTTGCTTCAAGTAATCAACGCTGCACTCAACAACCAACTGTTCTTCAAGTTGGCCAATCACAAAATGACAGTTGTCGCCGTCGACGCCATGTACACCGACCCTTACGTCACCGACGTCATCGTTCTCGCCCCCGGCCAGACCACCGACGTCCTCGTCAAAGCCGACCAGCCCATCGGCTCCTACTACATGGCCGCCAGCCCCTACTCCGATCTCCGCCCGCCGATCCCATTCCCAAACTCCATCACACGCGCCGTCGTCATCTACGACGGCGCCTCGCCGTCCGCCACTCCCCGGATGCCGGTCTTACCGGCGTTCAACGACACGCCGACGGCGCACAGATTCTTCACCAACATAACCGCCCTCGCGGGGGCCCGCCACTGGGTCCCAGTGCCCGCCACGTGGACGAGCACATGTTCGTCACGTTCGGTGTGA

mRNA sequence

ATGGCTTCCGCTGCCATTCTCCAGCATTCTTTCACTGTGCAAGACATGACCGTCCGCCGGCTCTGCCGTGACCAAGTTATAACGGCCGTCAACGGAAATTCCCGGGTCCAACCATTCACGCTCAAGACGGCGACGTTCTGGTCGTCCACGCATGGGGTTTTCCAGCTGCTCAGCGCGTGGGCCGACGGGCCGGAGAACGTAACGCAATGTCCGATACGGTCCGGCGAAAACTACACGTACAGATTCAAGATCACCGGACAAGAGGGGACTCTGTGGTGGCACGCGCACTCGTCTTGGCTACGCGCCACCGTCCACGGCGCCCTCCTCATCGCCCCCAAGTCCGGCCGACCGTTGCCCTTCCCGAAACCTTACAGGCAGATTCCGATCTTGCTCGGAGAGTGGTGGAACGCCAACGTCGTTGAGGTTGAAGAGGGAGGTCTCGCTACCGGCGCCGGTCCGAATAACTCCGACGCCTACACCATTAATGCCCTCCCCGGAAATCTCTACCCTTGCTCGCAAAATCAAACCTATCAACTGAAGATGGTGCCCAAAAAGACATACTTGCTTCAAGTAATCAACGCTGCACTCAACAACCAACTGTTCTTCAAGTTGGCCAATCACAAAATGACAGTTGTCGCCGTCGACGCCATGTACACCGACCCTTACGTCACCGACGTCATCGTTCTCGCCCCCGGCCAGACCACCGACGTCCTCGTCAAAGCCGACCAGCCCATCGGCTCCTACTACATGGCCGCCAGCCCCTACTCCGATCTCCGCCCGCCGATCCCATTCCCAAACTCCATCACACGCGCCGTCGTCATCTACGACGGCGCCTCGCCGTCCGCCACTCCCCGGATGCCGGTCTTACCGGCGTTCAACGACACGCCGACGGCGCACAGATTCTTCACCAACATAACCGCCCTCGCGGGGGCCCGCCACTGGGTCCCAGTGCCCGCCACGTGGACGAGCACATGTTCGTCACGTTCGGTGTGA

Coding sequence (CDS)

ATGGCTTCCGCTGCCATTCTCCAGCATTCTTTCACTGTGCAAGACATGACCGTCCGCCGGCTCTGCCGTGACCAAGTTATAACGGCCGTCAACGGAAATTCCCGGGTCCAACCATTCACGCTCAAGACGGCGACGTTCTGGTCGTCCACGCATGGGGTTTTCCAGCTGCTCAGCGCGTGGGCCGACGGGCCGGAGAACGTAACGCAATGTCCGATACGGTCCGGCGAAAACTACACGTACAGATTCAAGATCACCGGACAAGAGGGGACTCTGTGGTGGCACGCGCACTCGTCTTGGCTACGCGCCACCGTCCACGGCGCCCTCCTCATCGCCCCCAAGTCCGGCCGACCGTTGCCCTTCCCGAAACCTTACAGGCAGATTCCGATCTTGCTCGGAGAGTGGTGGAACGCCAACGTCGTTGAGGTTGAAGAGGGAGGTCTCGCTACCGGCGCCGGTCCGAATAACTCCGACGCCTACACCATTAATGCCCTCCCCGGAAATCTCTACCCTTGCTCGCAAAATCAAACCTATCAACTGAAGATGGTGCCCAAAAAGACATACTTGCTTCAAGTAATCAACGCTGCACTCAACAACCAACTGTTCTTCAAGTTGGCCAATCACAAAATGACAGTTGTCGCCGTCGACGCCATGTACACCGACCCTTACGTCACCGACGTCATCGTTCTCGCCCCCGGCCAGACCACCGACGTCCTCGTCAAAGCCGACCAGCCCATCGGCTCCTACTACATGGCCGCCAGCCCCTACTCCGATCTCCGCCCGCCGATCCCATTCCCAAACTCCATCACACGCGCCGTCGTCATCTACGACGGCGCCTCGCCGTCCGCCACTCCCCGGATGCCGGTCTTACCGGCGTTCAACGACACGCCGACGGCGCACAGATTCTTCACCAACATAACCGCCCTCGCGGGGGCCCGCCACTGGGTCCCAGTGCCCGCCACGTGGACGAGCACATGTTCGTCACGTTCGGTGTGA

Protein sequence

MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNSRVQPFTLKTATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVPATWTSTCSSRSV
Homology
BLAST of Moc01g20460 vs. NCBI nr
Match: XP_022151516.1 (LOW QUALITY PROTEIN: laccase-7 [Momordica charantia])

HSP 1 Score: 609.8 bits (1571), Expect = 1.4e-170
Identity = 303/331 (91.54%), Postives = 303/331 (91.54%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNSR-------------VQPFTLKTATFW 60
           MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN               V  F        
Sbjct: 48  MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNXPGPTIHAQDGDVLVVHVFNKSPYDLT 107

Query: 61  SSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 120
              HGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA
Sbjct: 108 IHWHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 167

Query: 121 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 180
           LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN
Sbjct: 168 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 227

Query: 181 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 240
           LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI
Sbjct: 228 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 287

Query: 241 VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP 300
           VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP
Sbjct: 288 VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP 347

Query: 301 VLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           VLPAFNDTPTAHRFFTNITALAGARHWVPVP
Sbjct: 348 VLPAFNDTPTAHRFFTNITALAGARHWVPVP 378

BLAST of Moc01g20460 vs. NCBI nr
Match: XP_022978127.1 (laccase-7-like [Cucurbita maxima])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 260/336 (77.38%), Postives = 277/336 (82.44%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN------------------SRVQPFTLK 60
           MASAAI++HSFTVQDMTVRRLCRDQ ITAVNG                   S   P+ L 
Sbjct: 21  MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVSNKSPYDL- 80

Query: 61  TATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRA 120
               W   HGVFQLLS WADGPENVTQCPIR G NYTYRFKI  QEGTLWWHAHSSWLRA
Sbjct: 81  -TIHW---HGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRA 140

Query: 121 TVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTIN 180
           TVHGALLI PKSGRPLP+PKPY++IPILLGEWWNANVV VEE GLATG+GPN SDAYTIN
Sbjct: 141 TVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTIN 200

Query: 181 ALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPY 240
            LPGNLYPCSQNQTY LKM   KTYLLQVINAALNNQ FFKLANH +TVVAVDA+YTDPY
Sbjct: 201 GLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPY 260

Query: 241 VTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSA 300
           VTDVIVLAPGQTTDVLVKADQP  SYYMAA PY+D RPPI F ++ITRA+V+Y+GASPSA
Sbjct: 261 VTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYNGASPSA 320

Query: 301 TPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            P MP LP FNDTPTAH+F+TNITAL GARHWVPVP
Sbjct: 321 APLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVP 351

BLAST of Moc01g20460 vs. NCBI nr
Match: XP_023543234.1 (laccase-7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522.3 bits (1344), Expect = 3.0e-144
Identity = 258/336 (76.79%), Postives = 277/336 (82.44%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN------------------SRVQPFTLK 60
           MASAAI++HSFTVQDMTVRRLCRDQ ITAVNG                   S   P+ L 
Sbjct: 1   MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHAHDGDVLVVHVSNKSPYDL- 60

Query: 61  TATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRA 120
               W   HG+FQ LSAWADGPENVTQCPIR G NYTYRFKI  QEGTLWWHAHSSWLRA
Sbjct: 61  -TIHW---HGIFQQLSAWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRA 120

Query: 121 TVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTIN 180
           TVHGALLI PK+GRPLP+PKPY++IPILLGEWWNANVV VEE GL TG+GPN SDAYTIN
Sbjct: 121 TVHGALLIRPKNGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLTTGSGPNISDAYTIN 180

Query: 181 ALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPY 240
            LPGNLYPCSQNQTY LKM   KTYLLQVINAALNNQ FFKLANH +TVVAVDA+YTDPY
Sbjct: 181 GLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPY 240

Query: 241 VTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSA 300
           VTDVIVLAPGQTTDVLVKADQP  SYYMAA PY+D RPPI F ++ITRA+V+YDGASPSA
Sbjct: 241 VTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYDGASPSA 300

Query: 301 TPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            P MP LP+FNDTPTAH+F+TNITAL GARHWVPVP
Sbjct: 301 APLMPTLPSFNDTPTAHKFYTNITALVGARHWVPVP 331

BLAST of Moc01g20460 vs. NCBI nr
Match: XP_022950700.1 (laccase-7-like [Cucurbita moschata])

HSP 1 Score: 521.5 bits (1342), Expect = 5.1e-144
Identity = 257/336 (76.49%), Postives = 278/336 (82.74%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN------------------SRVQPFTLK 60
           MASAAI++HSFTVQDMTVRRLCRDQ ITAVN                    S   P+ L 
Sbjct: 19  MASAAIVEHSFTVQDMTVRRLCRDQEITAVNSEYPGPTIHAHDGDVLVVHVSNKSPYDL- 78

Query: 61  TATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRA 120
               W   HG+FQLLSAWADGPENVTQCPIR G NYTY+FKI  QEGTLWWHAHSSWLRA
Sbjct: 79  -TIHW---HGIFQLLSAWADGPENVTQCPIRPGGNYTYKFKIKEQEGTLWWHAHSSWLRA 138

Query: 121 TVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTIN 180
           TVHGALLI PK+GRPLP+PKPY++IPILLGEWWNANVV VEE GLATG+GPN SDAYTIN
Sbjct: 139 TVHGALLIRPKNGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTIN 198

Query: 181 ALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPY 240
            LPGNLYPCSQNQTY LKM   KTYLLQVINAALNNQ FFKLANH +TVVAVDA+YTDPY
Sbjct: 199 GLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPY 258

Query: 241 VTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSA 300
           VTDVIVLAPGQTTDVLVKADQP  SYYMAA PY+D RPPI F ++ITRA+V+YDGASPSA
Sbjct: 259 VTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYDGASPSA 318

Query: 301 TPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            P MP LP+FNDTPTAH+F+TNIT+L GARHWVPVP
Sbjct: 319 APLMPTLPSFNDTPTAHKFYTNITSLVGARHWVPVP 349

BLAST of Moc01g20460 vs. NCBI nr
Match: XP_008450212.1 (PREDICTED: laccase-7 [Cucumis melo])

HSP 1 Score: 519.6 bits (1337), Expect = 1.9e-143
Identity = 255/331 (77.04%), Postives = 277/331 (83.69%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNG-----NSRVQPFTLKTATFWSST----- 60
           M SAAI++HSFTV+DM+VRRLCRDQVITAVNG        VQ   +      +++     
Sbjct: 22  MVSAAIVEHSFTVEDMSVRRLCRDQVITAVNGEYPGPTIHVQDEDVLVVHVTNNSPHDLT 81

Query: 61  ---HGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 120
              HGVFQLLS WADGPEN+TQCPIR G+ YTYRFKI GQEGTLWWHAHSSWLRATVHGA
Sbjct: 82  IHWHGVFQLLSGWADGPENITQCPIRPGKKYTYRFKIKGQEGTLWWHAHSSWLRATVHGA 141

Query: 121 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 180
           LLI PK   PLP+P PY++IPILLGEWWNANVV+VE+ GLATG GPN SDAYTIN  PGN
Sbjct: 142 LLIRPKFDLPLPYPTPYKKIPILLGEWWNANVVQVEQEGLATGRGPNGSDAYTINGHPGN 201

Query: 181 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 240
           LYPCSQNQTY+LKMV  KTYLLQVIN ALNNQLFFKLANHK TVVAVDA YTDPY+TDVI
Sbjct: 202 LYPCSQNQTYELKMVRGKTYLLQVINVALNNQLFFKLANHKFTVVAVDATYTDPYITDVI 261

Query: 241 VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP 300
           VLAPGQTTDVLVKA+QPIGSYYMAA PY+D +P I FPNSITRAVVIYDGASPS TP MP
Sbjct: 262 VLAPGQTTDVLVKANQPIGSYYMAARPYADTQPQIGFPNSITRAVVIYDGASPSTTPIMP 321

Query: 301 VLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            LP FNDTPTAH+F+TNITAL GARHWVPVP
Sbjct: 322 TLPGFNDTPTAHKFYTNITALVGARHWVPVP 352

BLAST of Moc01g20460 vs. ExPASy Swiss-Prot
Match: Q9SR40 (Laccase-7 OS=Arabidopsis thaliana OX=3702 GN=LAC7 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.3e-107
Identity = 190/335 (56.72%), Postives = 242/335 (72.24%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNSRVQPFTLKTA-------------TFW 60
           + SA+I++H+F VQ++TV RLC+ QVIT VNG+       +K                  
Sbjct: 20  ITSASIVEHTFNVQNLTVSRLCKRQVITVVNGSLPGPTIRVKEGDSLVIHVLNHSPHNIT 79

Query: 61  SSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 120
              HG+F  L+ WADGP  +TQCPI+ G+ Y YRF ITGQEGTLWWHAH+S+LRATV+GA
Sbjct: 80  IHWHGIFHKLTVWADGPSMITQCPIQPGQRYAYRFNITGQEGTLWWHAHASFLRATVYGA 139

Query: 121 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 180
           L+I PKSG   PFPKP++++PIL GEWWN +VV +EE  +ATG  PNNSDAYTIN  PGN
Sbjct: 140 LVIRPKSGHSYPFPKPHKEVPILFGEWWNTDVVALEEAAIATGVPPNNSDAYTINGRPGN 199

Query: 181 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 240
           LYPCS+++ + L +V  K YLL++INAA+N QLFFK+ANH++TVVA DA+YT PYVTDVI
Sbjct: 200 LYPCSKDRMFSLNVVKGKRYLLRIINAAMNIQLFFKIANHRLTVVAADAVYTAPYVTDVI 259

Query: 241 VLAPGQTTDVLVKADQPIG-SYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPR- 300
           V+APGQT D L+ ADQ +  SYYMAA PY+   P +PFPN+ TR V+ Y GAS +   + 
Sbjct: 260 VIAPGQTIDALLFADQSVDTSYYMAAHPYAS-APAVPFPNTTTRGVIHYGGASKTGRSKP 319

Query: 301 --MPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
             MP LP+F DT TA+RF++N+TAL    HWVPVP
Sbjct: 320 VLMPKLPSFFDTLTAYRFYSNLTALVNGPHWVPVP 353

BLAST of Moc01g20460 vs. ExPASy Swiss-Prot
Match: Q9LFD1 (Laccase-9 OS=Arabidopsis thaliana OX=3702 GN=LAC9 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.1e-92
Identity = 171/335 (51.04%), Postives = 223/335 (66.57%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNG-------NSR---------VQPFTLKTA 60
           +ASAAI++H   V+D+ V  LC++Q+I  VNG       N R         +   T    
Sbjct: 22  IASAAIVEHVLHVKDVVVTPLCKEQMIPIVNGSLPGPTINVREGDTLVVHVINKSTYNVT 81

Query: 61  TFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATV 120
             W   HGVFQL S W DG   +TQCPI+   N+TY+F ITGQEGTL WHAH   LRAT+
Sbjct: 82  IHW---HGVFQLKSVWMDGANMITQCPIQPSNNFTYQFDITGQEGTLLWHAHVVNLRATI 141

Query: 121 HGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINAL 180
           HGAL+I P+SGRP PFPKPY+++P++  +WW+ +V  +E            SDAY IN L
Sbjct: 142 HGALIIRPRSGRPYPFPKPYKEVPLIFQQWWDTDVRLLELRPAPV------SDAYLINGL 201

Query: 181 PGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVT 240
            G+ YPCS+N+ + LK+V  KTYLL++INAALN  LFFK+ANH +TVVAVDA+YT PY+T
Sbjct: 202 AGDSYPCSKNRMFNLKVVQGKTYLLRIINAALNTHLFFKIANHNVTVVAVDAVYTTPYLT 261

Query: 241 DVIVLAPGQTTDVLVKADQPIGSYYMAASPY-SDLRPPIPFPNSITRAVVIYDGASPSAT 300
           DV++L PGQT D ++ ADQPIG+YYMA  PY S +  P       TR +++Y+GA+ S++
Sbjct: 262 DVMILTPGQTIDAILTADQPIGTYYMAIIPYFSAIGVPASPDTKPTRGLIVYEGATSSSS 321

Query: 301 PRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           P  P +P  ND PTAHRF +NIT+L G  HW PVP
Sbjct: 322 PTKPWMPPANDIPTAHRFSSNITSLVGGPHWTPVP 347

BLAST of Moc01g20460 vs. ExPASy Swiss-Prot
Match: Q9LFD2 (Laccase-8 OS=Arabidopsis thaliana OX=3702 GN=LAC8 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.8e-91
Identity = 167/335 (49.85%), Postives = 223/335 (66.57%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN----------------SRVQPFTLKTA 60
           +ASAA+++H   +QD+ V+ LC++Q+I A NG+                + +   T    
Sbjct: 22  IASAAVVEHVLHIQDVVVKPLCKEQIIPAANGSLPGPTINVREGDTLVVNVINNSTYNVT 81

Query: 61  TFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATV 120
             W   HGVFQL S W DG   +TQCPI+ G N+TY+F ITGQEGTL WHAH   LRAT+
Sbjct: 82  IHW---HGVFQLKSVWMDGANMITQCPIQPGYNFTYQFDITGQEGTLLWHAHVVNLRATL 141

Query: 121 HGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINAL 180
           HGAL+I P+SGRP PFPKPY+++PI+  +WW+ +V  ++            SDAY IN L
Sbjct: 142 HGALVIRPRSGRPYPFPKPYKEVPIVFQQWWDTDVRLLQLRPAPV------SDAYLINGL 201

Query: 181 PGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVT 240
            G+ YPCS+N+ + LK+V  KTYLL+++NAALN  LFFK+ANH +TVVAVDA+Y+ PY+T
Sbjct: 202 AGDSYPCSENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYSTPYLT 261

Query: 241 DVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPN-SITRAVVIYDGASPSAT 300
           DV++L PGQT D L+ ADQ IG YYMA  PY      IP P+   TR +++Y GA+ S++
Sbjct: 262 DVMILTPGQTVDALLTADQAIGKYYMATLPYIS-AIGIPTPDIKPTRGLIVYQGATSSSS 321

Query: 301 PRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           P  P++P  ND  TAHRF +NIT+L G  HW PVP
Sbjct: 322 PAEPLMPVPNDMSTAHRFTSNITSLVGGPHWTPVP 346

BLAST of Moc01g20460 vs. ExPASy Swiss-Prot
Match: Q9FLB5 (Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-83
Identity = 166/330 (50.30%), Postives = 205/330 (62.12%), Query Frame = 0

Query: 4   AAILQHSFTVQDMTVRRLCRDQVITAVNG----------NSRVQPFTLKTATFWSST--- 63
           A +  H F +Q+  V+RLC+ +    VNG          N       +     ++ T   
Sbjct: 24  AKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRARYNITIHW 83

Query: 64  HGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGALLI 123
           HGV Q+ + WADGPE VTQCPIR G++YTYRF I GQEGTLWWHAHSSWLRATV+GAL+I
Sbjct: 84  HGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRATVYGALII 143

Query: 124 APKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGNLYP 183
            P  G   PFPKP RQ  ++LGEWWNAN V+V      TGA PN SDAYTIN  PG+LY 
Sbjct: 144 HPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNISDAYTINGQPGDLYN 203

Query: 184 CSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVIVLA 243
           CS  +T  + +   +T LL+VINAALN  LFF +ANHK+TVV  DA Y  P+ T V++L 
Sbjct: 204 CSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLG 263

Query: 244 PGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMPVLP 303
           PGQTTDVL+ ADQP   YY+AA  Y   +   PF N+ T A++ Y   + ++ P MPVLP
Sbjct: 264 PGQTTDVLLTADQPPKRYYIAARAYQSAQ-NAPFDNTTTTAILQYKKTTTTSKPIMPVLP 323

Query: 304 AFNDTPTAHRFFTNITALAGARHWVPVPAT 321
           AFNDT T   F     +L      V VP T
Sbjct: 324 AFNDTNTVTSFSRKFKSLRN----VVVPKT 348

BLAST of Moc01g20460 vs. ExPASy Swiss-Prot
Match: Q941X2 (Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.4e-82
Identity = 163/339 (48.08%), Postives = 205/339 (60.47%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN----------------SRVQPFTLKTA 60
           +A A +  H F VQ+  V+RLC+   +  VNG                 + V        
Sbjct: 21  LAGAEVHHHEFIVQETPVKRLCKTHNVITVNGQLPGPTLEVREGDTVVINVVNHAQYNVT 80

Query: 61  TFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATV 120
             W   HG+ Q  + WADGPE VTQCPI+ G +Y YRF I GQEGTLWWHAHSSWLRATV
Sbjct: 81  IHW---HGIRQFRTGWADGPEFVTQCPIKPGGSYKYRFTIEGQEGTLWWHAHSSWLRATV 140

Query: 121 HGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINAL 180
           +GAL+I P+  +  PF KP R++P++LGEWW+A+ ++V      TGA PN SDAYTIN  
Sbjct: 141 YGALIIRPRENKTYPFEKPAREVPLILGEWWDADPIQVIREAQRTGAAPNISDAYTINGQ 200

Query: 181 PGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVT 240
           PG+LY CS+ +T  + + P +T LL+ INAALN +LF  +A HKMTVV VDA YT P+ T
Sbjct: 201 PGDLYNCSKEETTAVPVKPGETALLRFINAALNQELFVSIAQHKMTVVGVDASYTKPFTT 260

Query: 241 DVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYD-----GAS 300
            V+++APGQTTDVLV  DQ    YY+AA  Y D    + F N+ T AV+ YD        
Sbjct: 261 SVLMIAPGQTTDVLVTMDQAPTRYYLAARAY-DSAQGVAFDNTTTTAVIEYDCGCATDFG 320

Query: 301 PSATPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           PS  P  PVLPAFNDT TA  F   I     + H V +P
Sbjct: 321 PSIPPAFPVLPAFNDTNTATAFAAGIR----SPHEVKIP 351

BLAST of Moc01g20460 vs. ExPASy TrEMBL
Match: A0A6J1DEW8 (Laccase OS=Momordica charantia OX=3673 GN=LOC111019442 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 6.9e-171
Identity = 303/331 (91.54%), Postives = 303/331 (91.54%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNSR-------------VQPFTLKTATFW 60
           MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN               V  F        
Sbjct: 48  MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNXPGPTIHAQDGDVLVVHVFNKSPYDLT 107

Query: 61  SSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 120
              HGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA
Sbjct: 108 IHWHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 167

Query: 121 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 180
           LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN
Sbjct: 168 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 227

Query: 181 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 240
           LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI
Sbjct: 228 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 287

Query: 241 VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP 300
           VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP
Sbjct: 288 VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP 347

Query: 301 VLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           VLPAFNDTPTAHRFFTNITALAGARHWVPVP
Sbjct: 348 VLPAFNDTPTAHRFFTNITALAGARHWVPVP 378

BLAST of Moc01g20460 vs. ExPASy TrEMBL
Match: A0A6J1IKA9 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478200 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.5e-145
Identity = 260/336 (77.38%), Postives = 277/336 (82.44%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN------------------SRVQPFTLK 60
           MASAAI++HSFTVQDMTVRRLCRDQ ITAVNG                   S   P+ L 
Sbjct: 21  MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVSNKSPYDL- 80

Query: 61  TATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRA 120
               W   HGVFQLLS WADGPENVTQCPIR G NYTYRFKI  QEGTLWWHAHSSWLRA
Sbjct: 81  -TIHW---HGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRA 140

Query: 121 TVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTIN 180
           TVHGALLI PKSGRPLP+PKPY++IPILLGEWWNANVV VEE GLATG+GPN SDAYTIN
Sbjct: 141 TVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTIN 200

Query: 181 ALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPY 240
            LPGNLYPCSQNQTY LKM   KTYLLQVINAALNNQ FFKLANH +TVVAVDA+YTDPY
Sbjct: 201 GLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPY 260

Query: 241 VTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSA 300
           VTDVIVLAPGQTTDVLVKADQP  SYYMAA PY+D RPPI F ++ITRA+V+Y+GASPSA
Sbjct: 261 VTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYNGASPSA 320

Query: 301 TPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            P MP LP FNDTPTAH+F+TNITAL GARHWVPVP
Sbjct: 321 APLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVP 351

BLAST of Moc01g20460 vs. ExPASy TrEMBL
Match: A0A6J1GGH4 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453717 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.5e-144
Identity = 257/336 (76.49%), Postives = 278/336 (82.74%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN------------------SRVQPFTLK 60
           MASAAI++HSFTVQDMTVRRLCRDQ ITAVN                    S   P+ L 
Sbjct: 19  MASAAIVEHSFTVQDMTVRRLCRDQEITAVNSEYPGPTIHAHDGDVLVVHVSNKSPYDL- 78

Query: 61  TATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRA 120
               W   HG+FQLLSAWADGPENVTQCPIR G NYTY+FKI  QEGTLWWHAHSSWLRA
Sbjct: 79  -TIHW---HGIFQLLSAWADGPENVTQCPIRPGGNYTYKFKIKEQEGTLWWHAHSSWLRA 138

Query: 121 TVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTIN 180
           TVHGALLI PK+GRPLP+PKPY++IPILLGEWWNANVV VEE GLATG+GPN SDAYTIN
Sbjct: 139 TVHGALLIRPKNGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTIN 198

Query: 181 ALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPY 240
            LPGNLYPCSQNQTY LKM   KTYLLQVINAALNNQ FFKLANH +TVVAVDA+YTDPY
Sbjct: 199 GLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPY 258

Query: 241 VTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSA 300
           VTDVIVLAPGQTTDVLVKADQP  SYYMAA PY+D RPPI F ++ITRA+V+YDGASPSA
Sbjct: 259 VTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYDGASPSA 318

Query: 301 TPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            P MP LP+FNDTPTAH+F+TNIT+L GARHWVPVP
Sbjct: 319 APLMPTLPSFNDTPTAHKFYTNITSLVGARHWVPVP 349

BLAST of Moc01g20460 vs. ExPASy TrEMBL
Match: A0A1S3BPD5 (Laccase OS=Cucumis melo OX=3656 GN=LOC103491871 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.4e-144
Identity = 255/331 (77.04%), Postives = 277/331 (83.69%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNG-----NSRVQPFTLKTATFWSST----- 60
           M SAAI++HSFTV+DM+VRRLCRDQVITAVNG        VQ   +      +++     
Sbjct: 22  MVSAAIVEHSFTVEDMSVRRLCRDQVITAVNGEYPGPTIHVQDEDVLVVHVTNNSPHDLT 81

Query: 61  ---HGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 120
              HGVFQLLS WADGPEN+TQCPIR G+ YTYRFKI GQEGTLWWHAHSSWLRATVHGA
Sbjct: 82  IHWHGVFQLLSGWADGPENITQCPIRPGKKYTYRFKIKGQEGTLWWHAHSSWLRATVHGA 141

Query: 121 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 180
           LLI PK   PLP+P PY++IPILLGEWWNANVV+VE+ GLATG GPN SDAYTIN  PGN
Sbjct: 142 LLIRPKFDLPLPYPTPYKKIPILLGEWWNANVVQVEQEGLATGRGPNGSDAYTINGHPGN 201

Query: 181 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 240
           LYPCSQNQTY+LKMV  KTYLLQVIN ALNNQLFFKLANHK TVVAVDA YTDPY+TDVI
Sbjct: 202 LYPCSQNQTYELKMVRGKTYLLQVINVALNNQLFFKLANHKFTVVAVDATYTDPYITDVI 261

Query: 241 VLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMP 300
           VLAPGQTTDVLVKA+QPIGSYYMAA PY+D +P I FPNSITRAVVIYDGASPS TP MP
Sbjct: 262 VLAPGQTTDVLVKANQPIGSYYMAARPYADTQPQIGFPNSITRAVVIYDGASPSTTPIMP 321

Query: 301 VLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
            LP FNDTPTAH+F+TNITAL GARHWVPVP
Sbjct: 322 TLPGFNDTPTAHKFYTNITALVGARHWVPVP 352

BLAST of Moc01g20460 vs. ExPASy TrEMBL
Match: A0A6J1GEQ7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 2.0e-141
Identity = 253/336 (75.30%), Postives = 273/336 (81.25%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN------------------SRVQPFTLK 60
           MASAAI++HSFTV+DMTVRRLCRDQVITAVNG                   S   P+ L 
Sbjct: 18  MASAAIVEHSFTVEDMTVRRLCRDQVITAVNGEYPGPTIHVQDDDVLIVHVSNNSPYDL- 77

Query: 61  TATFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRA 120
               W   HGVFQLLSAWADGPENVTQCPIR G NYTYRF I GQEGTLWWHAHSSWLRA
Sbjct: 78  -TIHW---HGVFQLLSAWADGPENVTQCPIRPGGNYTYRFNIKGQEGTLWWHAHSSWLRA 137

Query: 121 TVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTIN 180
           TVHGALLI PKSG PLP+ KPY+++PILLGEWWNANVV VEE GLA G GPN SDAYTIN
Sbjct: 138 TVHGALLIRPKSGLPLPYQKPYKKVPILLGEWWNANVVHVEEEGLAAGRGPNGSDAYTIN 197

Query: 181 ALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPY 240
            LPGNLYPC QNQTYQLKM   KT LLQV+NAALNNQLFFKLANH +TVVAVDA YT PY
Sbjct: 198 GLPGNLYPCPQNQTYQLKMARGKTSLLQVVNAALNNQLFFKLANHNLTVVAVDATYTVPY 257

Query: 241 VTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSA 300
           VTDVIVLAPGQTTDVLV ADQP+GSYYMAA PY+D RP I FPN+ITRA+VIYDGA  ++
Sbjct: 258 VTDVIVLAPGQTTDVLVNADQPLGSYYMAARPYADTRPQIAFPNTITRAIVIYDGAPTNS 317

Query: 301 TPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           TP MP+LPAFNDTPTAH+F+TN+TAL GARHW P P
Sbjct: 318 TPIMPLLPAFNDTPTAHKFYTNLTALVGARHWDPAP 348

BLAST of Moc01g20460 vs. TAIR 10
Match: AT3G09220.1 (laccase 7 )

HSP 1 Score: 390.2 bits (1001), Expect = 1.7e-108
Identity = 190/335 (56.72%), Postives = 242/335 (72.24%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGNSRVQPFTLKTA-------------TFW 60
           + SA+I++H+F VQ++TV RLC+ QVIT VNG+       +K                  
Sbjct: 20  ITSASIVEHTFNVQNLTVSRLCKRQVITVVNGSLPGPTIRVKEGDSLVIHVLNHSPHNIT 79

Query: 61  SSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGA 120
              HG+F  L+ WADGP  +TQCPI+ G+ Y YRF ITGQEGTLWWHAH+S+LRATV+GA
Sbjct: 80  IHWHGIFHKLTVWADGPSMITQCPIQPGQRYAYRFNITGQEGTLWWHAHASFLRATVYGA 139

Query: 121 LLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGN 180
           L+I PKSG   PFPKP++++PIL GEWWN +VV +EE  +ATG  PNNSDAYTIN  PGN
Sbjct: 140 LVIRPKSGHSYPFPKPHKEVPILFGEWWNTDVVALEEAAIATGVPPNNSDAYTINGRPGN 199

Query: 181 LYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVI 240
           LYPCS+++ + L +V  K YLL++INAA+N QLFFK+ANH++TVVA DA+YT PYVTDVI
Sbjct: 200 LYPCSKDRMFSLNVVKGKRYLLRIINAAMNIQLFFKIANHRLTVVAADAVYTAPYVTDVI 259

Query: 241 VLAPGQTTDVLVKADQPIG-SYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPR- 300
           V+APGQT D L+ ADQ +  SYYMAA PY+   P +PFPN+ TR V+ Y GAS +   + 
Sbjct: 260 VIAPGQTIDALLFADQSVDTSYYMAAHPYAS-APAVPFPNTTTRGVIHYGGASKTGRSKP 319

Query: 301 --MPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
             MP LP+F DT TA+RF++N+TAL    HWVPVP
Sbjct: 320 VLMPKLPSFFDTLTAYRFYSNLTALVNGPHWVPVP 353

BLAST of Moc01g20460 vs. TAIR 10
Match: AT5G01050.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.5e-93
Identity = 171/335 (51.04%), Postives = 223/335 (66.57%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNG-------NSR---------VQPFTLKTA 60
           +ASAAI++H   V+D+ V  LC++Q+I  VNG       N R         +   T    
Sbjct: 22  IASAAIVEHVLHVKDVVVTPLCKEQMIPIVNGSLPGPTINVREGDTLVVHVINKSTYNVT 81

Query: 61  TFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATV 120
             W   HGVFQL S W DG   +TQCPI+   N+TY+F ITGQEGTL WHAH   LRAT+
Sbjct: 82  IHW---HGVFQLKSVWMDGANMITQCPIQPSNNFTYQFDITGQEGTLLWHAHVVNLRATI 141

Query: 121 HGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINAL 180
           HGAL+I P+SGRP PFPKPY+++P++  +WW+ +V  +E            SDAY IN L
Sbjct: 142 HGALIIRPRSGRPYPFPKPYKEVPLIFQQWWDTDVRLLELRPAPV------SDAYLINGL 201

Query: 181 PGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVT 240
            G+ YPCS+N+ + LK+V  KTYLL++INAALN  LFFK+ANH +TVVAVDA+YT PY+T
Sbjct: 202 AGDSYPCSKNRMFNLKVVQGKTYLLRIINAALNTHLFFKIANHNVTVVAVDAVYTTPYLT 261

Query: 241 DVIVLAPGQTTDVLVKADQPIGSYYMAASPY-SDLRPPIPFPNSITRAVVIYDGASPSAT 300
           DV++L PGQT D ++ ADQPIG+YYMA  PY S +  P       TR +++Y+GA+ S++
Sbjct: 262 DVMILTPGQTIDAILTADQPIGTYYMAIIPYFSAIGVPASPDTKPTRGLIVYEGATSSSS 321

Query: 301 PRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           P  P +P  ND PTAHRF +NIT+L G  HW PVP
Sbjct: 322 PTKPWMPPANDIPTAHRFSSNITSLVGGPHWTPVP 347

BLAST of Moc01g20460 vs. TAIR 10
Match: AT5G01040.1 (laccase 8 )

HSP 1 Score: 335.5 bits (859), Expect = 4.8e-92
Identity = 167/335 (49.85%), Postives = 223/335 (66.57%), Query Frame = 0

Query: 1   MASAAILQHSFTVQDMTVRRLCRDQVITAVNGN----------------SRVQPFTLKTA 60
           +ASAA+++H   +QD+ V+ LC++Q+I A NG+                + +   T    
Sbjct: 22  IASAAVVEHVLHIQDVVVKPLCKEQIIPAANGSLPGPTINVREGDTLVVNVINNSTYNVT 81

Query: 61  TFWSSTHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATV 120
             W   HGVFQL S W DG   +TQCPI+ G N+TY+F ITGQEGTL WHAH   LRAT+
Sbjct: 82  IHW---HGVFQLKSVWMDGANMITQCPIQPGYNFTYQFDITGQEGTLLWHAHVVNLRATL 141

Query: 121 HGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINAL 180
           HGAL+I P+SGRP PFPKPY+++PI+  +WW+ +V  ++            SDAY IN L
Sbjct: 142 HGALVIRPRSGRPYPFPKPYKEVPIVFQQWWDTDVRLLQLRPAPV------SDAYLINGL 201

Query: 181 PGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVT 240
            G+ YPCS+N+ + LK+V  KTYLL+++NAALN  LFFK+ANH +TVVAVDA+Y+ PY+T
Sbjct: 202 AGDSYPCSENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYSTPYLT 261

Query: 241 DVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPN-SITRAVVIYDGASPSAT 300
           DV++L PGQT D L+ ADQ IG YYMA  PY      IP P+   TR +++Y GA+ S++
Sbjct: 262 DVMILTPGQTVDALLTADQAIGKYYMATLPYIS-AIGIPTPDIKPTRGLIVYQGATSSSS 321

Query: 301 PRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVP 319
           P  P++P  ND  TAHRF +NIT+L G  HW PVP
Sbjct: 322 PAEPLMPVPNDMSTAHRFTSNITSLVGGPHWTPVP 346

BLAST of Moc01g20460 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 310.8 bits (795), Expect = 1.3e-84
Identity = 166/330 (50.30%), Postives = 205/330 (62.12%), Query Frame = 0

Query: 4   AAILQHSFTVQDMTVRRLCRDQVITAVNG----------NSRVQPFTLKTATFWSST--- 63
           A +  H F +Q+  V+RLC+ +    VNG          N       +     ++ T   
Sbjct: 24  AKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRARYNITIHW 83

Query: 64  HGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGALLI 123
           HGV Q+ + WADGPE VTQCPIR G++YTYRF I GQEGTLWWHAHSSWLRATV+GAL+I
Sbjct: 84  HGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRATVYGALII 143

Query: 124 APKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGNLYP 183
            P  G   PFPKP RQ  ++LGEWWNAN V+V      TGA PN SDAYTIN  PG+LY 
Sbjct: 144 HPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNISDAYTINGQPGDLYN 203

Query: 184 CSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVIVLA 243
           CS  +T  + +   +T LL+VINAALN  LFF +ANHK+TVV  DA Y  P+ T V++L 
Sbjct: 204 CSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLG 263

Query: 244 PGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGASPSATPRMPVLP 303
           PGQTTDVL+ ADQP   YY+AA  Y   +   PF N+ T A++ Y   + ++ P MPVLP
Sbjct: 264 PGQTTDVLLTADQPPKRYYIAARAYQSAQ-NAPFDNTTTTAILQYKKTTTTSKPIMPVLP 323

Query: 304 AFNDTPTAHRFFTNITALAGARHWVPVPAT 321
           AFNDT T   F     +L      V VP T
Sbjct: 324 AFNDTNTVTSFSRKFKSLRN----VVVPKT 348

BLAST of Moc01g20460 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 303.9 bits (777), Expect = 1.6e-82
Identity = 162/329 (49.24%), Postives = 199/329 (60.49%), Query Frame = 0

Query: 9   HSFTVQDMTVRRLCRDQVITAVNG----------NSRVQPFTLKTATFWSST---HGVFQ 68
           H F +Q   V+RLC       VNG          N       +     ++ T   HGV Q
Sbjct: 31  HEFIIQATKVKRLCETHNSITVNGMFPGPMLVVNNGDTLVVKVINRARYNITIHWHGVRQ 90

Query: 69  LLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAHSSWLRATVHGALLIAPKSG 128
           + + WADGPE VTQCPIR G +YTYRF I GQEGTLWWHAHSSWLRATV+G+LL+ P +G
Sbjct: 91  MRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQEGTLWWHAHSSWLRATVYGSLLVFPPAG 150

Query: 129 RPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNSDAYTINALPGNLYPCSQNQ 188
              PF KP+R +P+LLGEWW+AN V+V    + TG  PNNSDAYTIN  PG+LY CS   
Sbjct: 151 SSYPFTKPHRNVPLLLGEWWDANPVDVLRESIRTGGAPNNSDAYTINGQPGDLYKCSSQD 210

Query: 189 TYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDAMYTDPYVTDVIVLAPGQTT 248
           T  + +   +T LL+VIN+ALN  LFF +ANHK+TVV  DA Y  P+ T+VIVL PGQTT
Sbjct: 211 TTVVPINVGETILLRVINSALNQPLFFTVANHKLTVVGADASYLKPFTTNVIVLGPGQTT 270

Query: 249 DVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYDGA-------------SPSA 308
           DVL+  DQP   YYMAA  Y   +   PF N+ T A++ Y  A               S 
Sbjct: 271 DVLITGDQPPNRYYMAARAYQSAQ-NAPFGNTTTTAILQYKSAPCCGVGGGSGTKKGNSF 330

Query: 309 TPRMPVLPAFNDTPTAHRFFTNITALAGA 312
            P MP+LPA+NDT T  RF  +  +L  A
Sbjct: 331 KPIMPILPAYNDTNTVTRFSQSFRSLRRA 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151516.11.4e-17091.54LOW QUALITY PROTEIN: laccase-7 [Momordica charantia][more]
XP_022978127.11.3e-14477.38laccase-7-like [Cucurbita maxima][more]
XP_023543234.13.0e-14476.79laccase-7 [Cucurbita pepo subsp. pepo][more]
XP_022950700.15.1e-14476.49laccase-7-like [Cucurbita moschata][more]
XP_008450212.11.9e-14377.04PREDICTED: laccase-7 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9SR402.3e-10756.72Laccase-7 OS=Arabidopsis thaliana OX=3702 GN=LAC7 PE=2 SV=1[more]
Q9LFD12.1e-9251.04Laccase-9 OS=Arabidopsis thaliana OX=3702 GN=LAC9 PE=2 SV=1[more]
Q9LFD26.8e-9149.85Laccase-8 OS=Arabidopsis thaliana OX=3702 GN=LAC8 PE=2 SV=1[more]
Q9FLB51.8e-8350.30Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1[more]
Q941X24.4e-8248.08Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DEW86.9e-17191.54Laccase OS=Momordica charantia OX=3673 GN=LOC111019442 PE=3 SV=1[more]
A0A6J1IKA96.5e-14577.38Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478200 PE=3 SV=1[more]
A0A6J1GGH42.5e-14476.49Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453717 PE=3 SV=1[more]
A0A1S3BPD59.4e-14477.04Laccase OS=Cucumis melo OX=3656 GN=LOC103491871 PE=3 SV=1[more]
A0A6J1GEQ72.0e-14175.30Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G09220.11.7e-10856.72laccase 7 [more]
AT5G01050.11.5e-9351.04Laccase/Diphenol oxidase family protein [more]
AT5G01040.14.8e-9249.85laccase 8 [more]
AT5G05390.11.3e-8450.30laccase 12 [more]
AT2G40370.11.6e-8249.24laccase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 125..311
e-value: 2.4E-40
score: 140.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 14..119
e-value: 7.2E-17
score: 63.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 8..137
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 120..310
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 49..113
e-value: 6.1E-20
score: 71.4
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 127..277
e-value: 1.2E-37
score: 129.5
NoneNo IPR availablePANTHERPTHR11709:SF9LACCASE-7coord: 2..320
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 2..320
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 127..275
e-value: 3.59682E-85
score: 251.364

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g20460.1Moc01g20460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity