Moc01g16960 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc01g16960
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Reverse transcriptase
Location	chr1: 11351199 .. 11351564 (-)
RNA-Seq Expression	Moc01g16960
Synteny	Moc01g16960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTACCAGGAGACTTTGAAGAGTGCTCTGCTATAAATAACTTGAATCCTGTTATGTTTGATGAGTTTTATGACTTGTTAGTTACAGAGATTGAAGAAGAGCTTGATAAGATAGCAGAAGGACCGGAAGATGTGACTAATCCTGTTGAAAAAATACAAAAAGAAGAATGCAAGTCGTTACTTCCGTCCATAGTGGAACCACCCACTTTGGAGCAGAAGCCATTGCCGTCGCATTTGAAATATGCGTATCTAGGGGATAACGACACTTTACCAGTTATTATAGCTTCCAATTTATCACCTACTAATGAATATTCTTTATTACAGATTTTGGAGAAGCACAAAAAGGCCATTGATGGACGATAG

mRNA sequence

ATGAAATTACCAGGAGACTTTGAAGAGTGCTCTGCTATAAATAACTTGAATCCTGTTATGTTTGATGAGTTTTATGACTTGTTAGTTACAGAGATTGAAGAAGAGCTTGATAAGATAGCAGAAGGACCGGAAGATGTGACTAATCCTGTTGAAAAAATACAAAAAGAAGAATGCAAGTCGTTACTTCCGTCCATAGTGGAACCACCCACTTTGGAGCAGAAGCCATTGCCGTCGCATTTGAAATATGCGTATCTAGGGGATAACGACACTTTACCAGTTATTATAGCTTCCAATTTATCACCTACTAATGAATATTCTTTATTACAGATTTTGGAGAAGCACAAAAAGGCCATTGATGGACGATAG

Coding sequence (CDS)

ATGAAATTACCAGGAGACTTTGAAGAGTGCTCTGCTATAAATAACTTGAATCCTGTTATGTTTGATGAGTTTTATGACTTGTTAGTTACAGAGATTGAAGAAGAGCTTGATAAGATAGCAGAAGGACCGGAAGATGTGACTAATCCTGTTGAAAAAATACAAAAAGAAGAATGCAAGTCGTTACTTCCGTCCATAGTGGAACCACCCACTTTGGAGCAGAAGCCATTGCCGTCGCATTTGAAATATGCGTATCTAGGGGATAACGACACTTTACCAGTTATTATAGCTTCCAATTTATCACCTACTAATGAATATTCTTTATTACAGATTTTGGAGAAGCACAAAAAGGCCATTGATGGACGATAG

Protein sequence

MKLPGDFEECSAINNLNPVMFDEFYDLLVTEIEEELDKIAEGPEDVTNPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKAIDGR

Homology

[Close]

BLAST of Moc01g16960 vs. NCBI nr
Match: XP_022151603.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 [Momordica charantia])

HSP 1 Score: 171.0 bits (432), Expect = 6.2e-39
Identity = 85/88 (96.59%), Postives = 87/88 (98.86%), Query Frame = 0


Query: 31  EIEEELDKIAEGPEDVTNPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT 90
           EIEEELDKIAEGPEDVT+PVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT
Sbjct: 35  EIEEELDKIAEGPEDVTDPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT 94

Query: 91  LPVIIASNLSPTNEYSLLQILEKHKKAI 119
           LPVI+ SNLSPTNEYSLLQILEKHKKAI
Sbjct: 95  LPVIJXSNLSPTNEYSLLQILEKHKKAI 122

[Close]

BLAST of Moc01g16960 vs. NCBI nr
Match: XP_022158979.1 (uncharacterized protein LOC111025424 [Momordica charantia])

HSP 1 Score: 113.2 bits (282), Expect = 1.5e-21
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 63  PSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKAI 119
           P+IVEPPTLEQKPLPSHLKYAYLGDN+TLPVIIASNLSPTNEYSLLQILEKHKKAI
Sbjct: 204 PTIVEPPTLEQKPLPSHLKYAYLGDNBTLPVIIASNLSPTNEYSLLQILEKHKKAI 259

[Close]

BLAST of Moc01g16960 vs. NCBI nr
Match: XP_030502183.1 (uncharacterized protein LOC115717351 [Cannabis sativa])

HSP 1 Score: 97.4 bits (241), Expect = 8.7e-17
Identity = 60/138 (43.48%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 1   MKLPGDFEECSAINNLNPVMFDEFY-----DLLVTEIEEELDKIAEGPEDVTNPVEKIQ- 60
           M+ P + EECS I+ ++ ++ ++F+     D       +EL+ ++E  ++    VE +Q 
Sbjct: 275 MRFPDEIEECSRISVIDSIVAEKFHKEACKDEKFISSFDELEDLSEDEDNQVAWVEPLQP 334

Query: 61  ----KEECKSL----------LPSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLS 119
               K+  +SL           PS  EPP LE KPLPSHLKYAYLG+NDTLPVIIASNL 
Sbjct: 335 MPKFKKPFESLELKESNFKPPKPSTQEPPKLELKPLPSHLKYAYLGENDTLPVIIASNLV 394

[Close]

BLAST of Moc01g16960 vs. NCBI nr
Match: XP_030504461.1 (uncharacterized protein LOC115719521 [Cannabis sativa])

HSP 1 Score: 94.4 bits (233), Expect = 7.4e-16
Identity = 56/138 (40.58%), Postives = 81/138 (58.70%), Query Frame = 0


Query: 1   MKLPGDFEECSAINNLNPVMFDEFY-----DLLVTEIEEELDKIAEGPEDVTNPVEKIQ- 60
           M+ P + EECS ++ ++ ++ + F+     D  V    E+L+ ++E  E   + VE +Q 
Sbjct: 142 MRFPDEIEECSRLSVIDSIVAESFHKKVWKDERVISSLEDLEALSEDEETQVSWVEPMQP 201

Query: 61  --------------KEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLS 119
                         +   K L PSI EPP LE KPLPSHLKYAYLG+N+TLPVII++ L 
Sbjct: 202 FPKFKRAFESLELKESNFKPLKPSIQEPPKLELKPLPSHLKYAYLGENETLPVIISAWLE 261

[Close]

BLAST of Moc01g16960 vs. NCBI nr
Match: XP_030505517.1 (uncharacterized protein LOC115720508 [Cannabis sativa])

HSP 1 Score: 90.9 bits (224), Expect = 8.2e-15
Identity = 55/138 (39.86%), Postives = 80/138 (57.97%), Query Frame = 0


Query: 1   MKLPGDFEECSAINNLNPVMFDEFY-----DLLVTEIEEELDKIAEGPEDVTNPVEKIQ- 60
           M+   + EECS I+ ++ ++ + F+     D       +EL+ ++E  ++    VE +Q 
Sbjct: 1   MRFLDEIEECSRISVIDSIVVERFHKEAWKDEKFISSFDELEDLSEDEDNQVAWVEPLQP 60

Query: 61  --------------KEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLS 119
                         +   K   PSI EPP LE KPLPSHLKYAYLG+NDTLPVIIA+NL 
Sbjct: 61  IPKFKKPFESLELKESNVKPPKPSIQEPPKLELKPLPSHLKYAYLGENDTLPVIIAANLG 120

[Close]

BLAST of Moc01g16960 vs. ExPASy TrEMBL
Match: A0A6J1DCL7 (LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia OX=3673 GN=LOC111019515 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.9e-39
Identity = 85/88 (96.59%), Postives = 87/88 (98.86%), Query Frame = 0


Query: 31  EIEEELDKIAEGPEDVTNPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT 90
           EIEEELDKIAEGPEDVT+PVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT
Sbjct: 35  EIEEELDKIAEGPEDVTDPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT 94

Query: 91  LPVIIASNLSPTNEYSLLQILEKHKKAI 119
           LPVI+ SNLSPTNEYSLLQILEKHKKAI
Sbjct: 95  LPVILXSNLSPTNEYSLLQILEKHKKAI 122

[Close]

BLAST of Moc01g16960 vs. ExPASy TrEMBL
Match: A0A6J1E110 (uncharacterized protein LOC111025424 OS=Momordica charantia OX=3673 GN=LOC111025424 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.4e-22
Identity = 55/56 (98.21%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 63  PSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKAI 119
           P+IVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKAI
Sbjct: 204 PTIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKAI 259

[Close]

BLAST of Moc01g16960 vs. ExPASy TrEMBL
Match: A0A6P6UH48 (uncharacterized protein LOC113711211 OS=Coffea arabica OX=13443 GN=LOC113711211 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.5e-14
Identity = 40/68 (58.82%), Postives = 54/68 (79.41%), Query Frame = 0


Query: 51  EKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQI 110
           E + ++E + +LPS+V+ P LE KPLP HLKYAYLGDN+TLPVII+S LS T E  L+++
Sbjct: 248 ETVGRDELERVLPSVVQAPVLELKPLPEHLKYAYLGDNETLPVIISSALSKTQEEKLIRV 307

Query: 111 LEKHKKAI 119
           L +HK+AI
Sbjct: 308 LREHKEAI 315

[Close]

BLAST of Moc01g16960 vs. ExPASy TrEMBL
Match: A0A2G9GK35 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_21798 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.8e-13
Identity = 58/135 (42.96%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 1   MKLPGDFEECSAIN---NL--NPVMFDEFYDLLVTEIEEELDKIAEGPEDV--------- 60
           MK P + +EC A+N   NL  N  + ++  D L   + + LD+  E   +V         
Sbjct: 522 MKFPNESDECFAVNLFDNLAGNESIAEQSLDPLERALLDLLDEENEEDYEVVKTLDASKY 581

Query: 61  --TNPVEKIQK-EECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTN 119
             +  VE +++    K L PSI EPPTLE KPLPSHL YAYLG++DTLPVII+S+LS   
Sbjct: 582 FKSRGVESLERIAPSKVLKPSIEEPPTLELKPLPSHLCYAYLGESDTLPVIISSSLSDLQ 641

[Close]

BLAST of Moc01g16960 vs. ExPASy TrEMBL
Match: A0A6J1E3L7 (uncharacterized protein LOC111025754 OS=Momordica charantia OX=3673 GN=LOC111025754 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 3.7e-13
Identity = 40/52 (76.92%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 1  MKLPGDFEECSAINNLNPVMFDEFYDLLVTEIEEELDKIAEGPEDVTNPVEK 53
          MKLPGDFEECSAI NLNPVMFDEFYDLLV EIE+E DKIAEGP+++    +K
Sbjct: 1  MKLPGDFEECSAITNLNPVMFDEFYDLLVREIEKEHDKIAEGPKEILEKHKK 52

The following BLAST results are available for this feature:

BLAST of Moc01g16960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151603.1	6.2e-39	96.59	LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 [Momordica charantia]	[more]
XP_022158979.1	1.5e-21	96.43	uncharacterized protein LOC111025424 [Momordica charantia]	[more]
XP_030502183.1	8.7e-17	43.48	uncharacterized protein LOC115717351 [Cannabis sativa]	[more]
XP_030504461.1	7.4e-16	40.58	uncharacterized protein LOC115719521 [Cannabis sativa]	[more]
XP_030505517.1	8.2e-15	39.86	uncharacterized protein LOC115720508 [Cannabis sativa]	[more]

BLAST of Moc01g16960 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc01g16960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCL7	3.9e-39	96.59	LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia...	[more]
A0A6J1E110	4.4e-22	98.21	uncharacterized protein LOC111025424 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6P6UH48	7.5e-14	58.82	uncharacterized protein LOC113711211 OS=Coffea arabica OX=13443 GN=LOC113711211 ...	[more]
A0A2G9GK35	2.8e-13	42.96	Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_21798 PE=...	[more]
A0A6J1E3L7	3.7e-13	76.92	uncharacterized protein LOC111025754 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]

BLAST of Moc01g16960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR24559:SF373	REVERSE TRANSCRIPTASE DOMAIN, RIBONUCLEASE H-LIKE DOMAIN PROTEIN-RELATED	coord: 35..118
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 35..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc01g16960.1	Moc01g16960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding