Moc01g16940 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g16940
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionGlycerol-3-phosphate dehydrogenase [NAD(+)]
Locationchr1: 11328055 .. 11331897 (-)
RNA-Seq ExpressionMoc01g16940
SyntenyMoc01g16940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCACCATCCTCTCTCACATTCAAATGGTTCAACTCATAACTGCAATGGATTGGAAGAGAAGCTTGAAGAACTCAGAAGACTTCTAGGCAAATCTGATGGCGATCCACTGAGGATCGTTGGGGTGGGAGCTGGAGCTTGGGGAAGTGTTTTTGCAGCTCTGCTTCAAGACAGCTATGGCCAATTTCGGGAGAAGGTTCAAATCCGGATATGGAGAAGAGCAGGAAGAGTTGTGGATAAAGCTACAGCAGAACACCTCTTTGAAGTGATAAACTCAAGGGAAGATGTGCTTAGGAGGCTCATCAGGAGATGTGCTTATCTTAAATATGTTGAAGCTCGGCTCGGGGACCGGGTGCTTTATGCGGATGAGATTTTGAAAGACGGGTTTTGCTTGAACATGATTGACACACCCCTTTGCCCTTTGAAGGTGGTGTCTAACTTGCAGGAGGCTGTTTGGGATGCTGACATTGTGGTCAATGGCCTGCCCTCTACTGAAACAAGGGAGGTTTTTGAAGAGATAAGGAAATATTGGAAGGAGAGAATCACAGTGCCTATTATAATCTCTTTGGCAAAGGGAATAGAAGCTGCTCTGAAACCTGTTCCCCACATAATAACTCCTACTCTGATGATCAATCGAGCGAGTAAGACATTCGTCCCAAGTTTGTCATTTCATCTGTTCATTTGCATAACATGATCATTAAATGTGGGGATTACATTTTGTTTTGTATGTTAAAAAAATGAAAATAAACAGATAAGAACGCTGCAAACTGAAAAGGAACTATTGCCTGTATGGTTTTTCTGAGTTCATAGCTGGGAAAAGAATGTTATTTCTGAAGAATTTATTGACTGCGACGAACATTTTTCTTAACAGCTCGTTAGCTCAAATTCGAGAAGTCTAGATTAAGAAGTATGTGTGTGACTGTTTCTTAAAAATAAAAAAGAAGTATGTGCTGATTGTTAGTCTAGTGGGTTTGGGTTTGTGAAATTAATCTAAAGGTATTATTTGTGGCAAATTGTTAAGCATATGAATTGCCCTATAAAACTGAATTTGATACATTTGGTTCTCTCTGGAATTTTGTTTTTTTAGTAATAATTTTGTAGTCTCGTGATCCTGATGTTAACTGTTACTGAATTGTTAGGAATGATAAATATTTGGAGGGAACAAAAAGACCCCTACTGATAATTCCTATCTCTTCTTCTTCCTTTGCATTTGTCTGTGCAAATTCAGAGAACCTTTGCTTCAAATTTTCTCATATTTAACTTTGAATTCTCCCAGCTGGAGTGCCTATAGAGAATATATTGTATCTTGGTGGACCAAATATTGCCTCGGAGATCTACAACAAGGAATATGCTAATGCTCGAATTTGCGGAGCTGAAAAGTGGAGGAAACCCCTTGCAAACTTTTTGAGGCAACCTCAATTTATTGTCTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGCGGTCTGAAAAACGTCTATGCTATTGGAGCAGGCAAGTGGTAGATACTTTCTGGTTAATAATCTCAATAGTGATGGAAATTACCTCTCTCTATCTCTTATGTTACATGTTTGAAATGTGATTTTATAGACATTGAGCAAGTTATCTCTTCATACTTCATCAATTGTTTATAAGATTTTGAAACAATATGCCATCTGAATCCTTCTCTACAGAAGAAAGGAAAATGAGCGATAACAAATTGGCAACCAGTTTGATTGCAACAATTTATATATTAGACCTTCCATATTTCACTAAAATTATGTTTTTCAACCTCCTAGCTTGCATTGTAAGGTAAAGTCTGTTGTGATTGATTATCTACAATTGTTAGAGACTCGCCGAGCCTAAGTTATTAATATCAAAACGAGAAGCGAATTACAAACTCTTTGAGAGGCCTGGACTCTTCTTATTCTAGGGAACTCTTTTAGTCCCTAGGCCACGCATTGATCACTAAATATTCTACCCCGTCATACCTTAGAACTCAGGGATACAGTAGTACAATCTATAGAATAGATCTTGTTGACGTAAAATCTGTCACACCTCACCACTTAGTACTTATTCCCTATCCTAACTGTTGTTACAACCTGCAATTTTCTTTCTATATTCATTGTTATTCCGTTTTGAATTTTCAGGAATGGTGGCAGCCCTTACAAATGAGAGTGCCACCAGCAAATCAGTATATTTTGCACACTGTACCTCAGAGATGATCTTTATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTTGCAGGTCCTTTGCTCGCCGACACATACGTGACCTTGTTGAAAGGTCGTAATGCCTGGTACGGGCAAATGTTGGCCAAGGGTGAACTTAGTCTAGATATGGGTGATAGCATTAGCGGGAAAGGAATGATTCAGGTAATCGACTGCAATGTTTACCGCTTTTGTTTCTTGCTCCGTTACAATCTCCCCATCTTTTACATAGTTCAAATATGGAAAATCATTTGTAAGCGCGATGTGTGGCAGGCACGTGCGATGTTTTCTCGGTTAAAACTTTACGAACTATTTTCCGGAGTGATAATTTTTTAAAGCAATTCCTATACTTTGGTAATGGTCTTACAACACCCTCTTCTATTTTGCAGGGAGTGTCTGCTGTTAGTGCTTTCTATGAAATTCTGAGTCAATCAAGCCTAAATGTATTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGGAGCTTTGTCCCATCTTAAAGACACTATATAAAATACTGATTAAAAGGTAAACTTCATAATGCTCGTTTAGCATATAAATATTAGTTAGATTATAAGTTTGGTCAATGAATTTTACAATGAAAAAACTTCTGTTAGTTGTAAGTTTTGTCGAATAGGTCATTAAACACTAAAAATTAGATCATTCTTTGAACTTTCAATGCTGTATCTAATAGGTCAGGTCTTTGAACTTAAAAAAAATGTCCGATTGATACATCAACTTCCAATTTTATATGGAATAGGTCTTTGGTTTTTCAATTTTAGAACCCAGATTTACGAGACATTTTTTTAAATTCAGGGACTGTTTATTTTTATTTTCTTTTATAAGAAACCAAACTTTCATTGAATATACGAGAAAAAAACAAATACAACAAAAAGGGATCCACCTATAAAAACGAACTGCAATCCAAAAAATAACAGTTAACTGGCTACAAAAAAGCCTTAGAAACTGACAAAAATAGGGAGGTACTAAATCTAACAAACTCATAATTTCTACGGTAGACCTCTCAACTCCCCTAAAAATTCTACTATTCCTCTTAATCTAAATACCCCATGAAATAGTAAAAAAAAACGGGGGGGTTTGCCAAACAGGATCTCTCTATCAGAAAAGGGAGAAACAACCGATTCAAAGACTTATTAGACACAAACATAATAATTCCTGGACTTATTAGACATTTTTAAAGTTCCAAGGTTCTATTAGACATAACTTTGAAAGTTTAAGAATCTATTAGGTTTTTTTTTTTTTTTTAAGTTTCGAGACTTCTTAGATACAACTCTCAAAGTTGGAGGATCAAACTTGTAATTTAACCTAAATATCATTTTGAAATTTGCACATTGATATTCTAAAAATAAGTTGATATGAACTAGTTCATACAAGATCTTGCTCGTTTAACATTTTCTTTCATTTGAACCCCTAGAGTGAGCTTAAATATCAACGTTTTTCAAACACCATTTCTCTTTTCTCCGTGTTTTGGGTTTTTAGGGATGGATCGCCACAAGCTATACTTGAAGCATTGCGAGACGAGACCTTGAACGATCCCCGAGACCGCATCGAGTTGGCACAGAGCCATGCTTTCTACAGGCCTTCACTGCTTGGCCAACCTTGA

mRNA sequence

ATGAGTCACCATCCTCTCTCACATTCAAATGGTTCAACTCATAACTGCAATGGATTGGAAGAGAAGCTTGAAGAACTCAGAAGACTTCTAGGCAAATCTGATGGCGATCCACTGAGGATCGTTGGGGTGGGAGCTGGAGCTTGGGGAAGTGTTTTTGCAGCTCTGCTTCAAGACAGCTATGGCCAATTTCGGGAGAAGGTTCAAATCCGGATATGGAGAAGAGCAGGAAGAGTTGTGGATAAAGCTACAGCAGAACACCTCTTTGAAGTGATAAACTCAAGGGAAGATGTGCTTAGGAGGCTCATCAGGAGATGTGCTTATCTTAAATATGTTGAAGCTCGGCTCGGGGACCGGGTGCTTTATGCGGATGAGATTTTGAAAGACGGGTTTTGCTTGAACATGATTGACACACCCCTTTGCCCTTTGAAGGTGGTGTCTAACTTGCAGGAGGCTGTTTGGGATGCTGACATTGTGGTCAATGGCCTGCCCTCTACTGAAACAAGGGAGGTTTTTGAAGAGATAAGGAAATATTGGAAGGAGAGAATCACAGTGCCTATTATAATCTCTTTGGCAAAGGGAATAGAAGCTGCTCTGAAACCTGTTCCCCACATAATAACTCCTACTCTGATGATCAATCGAGCGACTGGAGTGCCTATAGAGAATATATTGTATCTTGGTGGACCAAATATTGCCTCGGAGATCTACAACAAGGAATATGCTAATGCTCGAATTTGCGGAGCTGAAAAGTGGAGGAAACCCCTTGCAAACTTTTTGAGGCAACCTCAATTTATTGTCTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGCGGTCTGAAAAACGTCTATGCTATTGGAGCAGGAATGGTGGCAGCCCTTACAAATGAGAGTGCCACCAGCAAATCAGTATATTTTGCACACTGTACCTCAGAGATGATCTTTATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTTGCAGGTCCTTTGCTCGCCGACACATACGTGACCTTGTTGAAAGGTCGTAATGCCTGGTACGGGCAAATGTTGGCCAAGGGTGAACTTAGTCTAGATATGGGTGATAGCATTAGCGGGAAAGGAATGATTCAGGGAGTGTCTGCTGTTAGTGCTTTCTATGAAATTCTGAGTCAATCAAGCCTAAATGTATTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGGAGCTTTGTCCCATCTTAAAGACACTATATAAAATACTGATTAAAAGGGATGGATCGCCACAAGCTATACTTGAAGCATTGCGAGACGAGACCTTGAACGATCCCCGAGACCGCATCGAGTTGGCACAGAGCCATGCTTTCTACAGGCCTTCACTGCTTGGCCAACCTTGA

Coding sequence (CDS)

ATGAGTCACCATCCTCTCTCACATTCAAATGGTTCAACTCATAACTGCAATGGATTGGAAGAGAAGCTTGAAGAACTCAGAAGACTTCTAGGCAAATCTGATGGCGATCCACTGAGGATCGTTGGGGTGGGAGCTGGAGCTTGGGGAAGTGTTTTTGCAGCTCTGCTTCAAGACAGCTATGGCCAATTTCGGGAGAAGGTTCAAATCCGGATATGGAGAAGAGCAGGAAGAGTTGTGGATAAAGCTACAGCAGAACACCTCTTTGAAGTGATAAACTCAAGGGAAGATGTGCTTAGGAGGCTCATCAGGAGATGTGCTTATCTTAAATATGTTGAAGCTCGGCTCGGGGACCGGGTGCTTTATGCGGATGAGATTTTGAAAGACGGGTTTTGCTTGAACATGATTGACACACCCCTTTGCCCTTTGAAGGTGGTGTCTAACTTGCAGGAGGCTGTTTGGGATGCTGACATTGTGGTCAATGGCCTGCCCTCTACTGAAACAAGGGAGGTTTTTGAAGAGATAAGGAAATATTGGAAGGAGAGAATCACAGTGCCTATTATAATCTCTTTGGCAAAGGGAATAGAAGCTGCTCTGAAACCTGTTCCCCACATAATAACTCCTACTCTGATGATCAATCGAGCGACTGGAGTGCCTATAGAGAATATATTGTATCTTGGTGGACCAAATATTGCCTCGGAGATCTACAACAAGGAATATGCTAATGCTCGAATTTGCGGAGCTGAAAAGTGGAGGAAACCCCTTGCAAACTTTTTGAGGCAACCTCAATTTATTGTCTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGCGGTCTGAAAAACGTCTATGCTATTGGAGCAGGAATGGTGGCAGCCCTTACAAATGAGAGTGCCACCAGCAAATCAGTATATTTTGCACACTGTACCTCAGAGATGATCTTTATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTTGCAGGTCCTTTGCTCGCCGACACATACGTGACCTTGTTGAAAGGTCGTAATGCCTGGTACGGGCAAATGTTGGCCAAGGGTGAACTTAGTCTAGATATGGGTGATAGCATTAGCGGGAAAGGAATGATTCAGGGAGTGTCTGCTGTTAGTGCTTTCTATGAAATTCTGAGTCAATCAAGCCTAAATGTATTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGGAGCTTTGTCCCATCTTAAAGACACTATATAAAATACTGATTAAAAGGGATGGATCGCCACAAGCTATACTTGAAGCATTGCGAGACGAGACCTTGAACGATCCCCGAGACCGCATCGAGTTGGCACAGAGCCATGCTTTCTACAGGCCTTCACTGCTTGGCCAACCTTGA

Protein sequence

MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Homology
BLAST of Moc01g16940 vs. NCBI nr
Match: XP_022153910.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic [Momordica charantia])

HSP 1 Score: 919.1 bits (2374), Expect = 1.5e-263
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSY 60
           MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSY
Sbjct: 1   MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSY 60

Query: 61  GQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVL 120
           GQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVL
Sbjct: 61  GQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVL 120

Query: 121 YADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKE 180
           YADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKE
Sbjct: 121 YADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKE 180

Query: 181 RITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYA 240
           RITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYA
Sbjct: 181 RITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYA 240

Query: 241 NARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESAT 300
           NARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESAT
Sbjct: 241 NARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESAT 300

Query: 301 SKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDM 360
           SKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDM
Sbjct: 301 SKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDM 360

Query: 361 GDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDG 420
           GDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDG
Sbjct: 361 GDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDG 420

Query: 421 SPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           SPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 421 SPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 458

BLAST of Moc01g16940 vs. NCBI nr
Match: XP_038883653.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic [Benincasa hispida])

HSP 1 Score: 896.0 bits (2314), Expect = 1.4e-256
Identity = 444/452 (98.23%), Postives = 451/452 (99.78%), Query Frame = 0

Query: 7   SHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREK 66
           SHSNGSTHNCNGLEEKL+ELRRL+GKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREK
Sbjct: 5   SHSNGSTHNCNGLEEKLDELRRLIGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREK 64

Query: 67  VQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEIL 126
           VQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEIL
Sbjct: 65  VQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEIL 124

Query: 127 KDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPI 186
           KDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETRE+FEEI KYWKERITVPI
Sbjct: 125 KDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREIFEEISKYWKERITVPI 184

Query: 187 IISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICG 246
           IISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICG
Sbjct: 185 IISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICG 244

Query: 247 AEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYF 306
           AEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYF
Sbjct: 245 AEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYF 304

Query: 307 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISG 366
           AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEL+LDMGDSISG
Sbjct: 305 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELNLDMGDSISG 364

Query: 367 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAIL 426
           KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAIL
Sbjct: 365 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAIL 424

Query: 427 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           EALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 425 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 456

BLAST of Moc01g16940 vs. NCBI nr
Match: XP_023544218.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.3 bits (2302), Expect = 3.4e-255
Identity = 443/453 (97.79%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65
           LSHSNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 40  LSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 99

Query: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 100 KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 159

Query: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 160 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 219

Query: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245
           IIISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC
Sbjct: 220 IIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 279

Query: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305
           GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 280 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 339

Query: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 340 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 399

Query: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 400 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 459

Query: 426 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 460 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 492

BLAST of Moc01g16940 vs. NCBI nr
Match: XP_022967787.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita maxima] >XP_022967788.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita maxima] >XP_023544219.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita pepo subsp. pepo] >XP_023544220.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.3 bits (2302), Expect = 3.4e-255
Identity = 443/453 (97.79%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65
           LSHSNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 3   LSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 62

Query: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 63  KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 122

Query: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 123 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 182

Query: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245
           IIISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC
Sbjct: 183 IIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 242

Query: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305
           GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 243 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 302

Query: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 303 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 362

Query: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 363 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 422

Query: 426 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 423 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of Moc01g16940 vs. NCBI nr
Match: XP_022967785.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita maxima] >XP_022967786.1 probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita maxima])

HSP 1 Score: 891.3 bits (2302), Expect = 3.4e-255
Identity = 443/453 (97.79%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65
           LSHSNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 40  LSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 99

Query: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 100 KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 159

Query: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 160 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 219

Query: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245
           IIISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC
Sbjct: 220 IIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 279

Query: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305
           GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 280 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 339

Query: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 340 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 399

Query: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 400 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 459

Query: 426 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 460 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 492

BLAST of Moc01g16940 vs. ExPASy Swiss-Prot
Match: O22216 (Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic OS=Arabidopsis thaliana OX=3702 GN=GPDHC1 PE=2 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 6.1e-231
Identity = 396/451 (87.80%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 9   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 68
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 69  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 128
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 129 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPII 188
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 189 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 248
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 249 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 308
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 309 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 368
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 369 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 428
           GMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

Query: 429 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 ALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of Moc01g16940 vs. ExPASy Swiss-Prot
Match: Q8S0G4 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0939600 PE=2 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 3.3e-229
Identity = 390/451 (86.47%), Postives = 421/451 (93.35%), Query Frame = 0

Query: 8   HSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 67
           H NGS +  NG EE+L+ELRRLLGKSDGD L+IVG+GAGAWGSVFAALLQD+YG+FREKV
Sbjct: 6   HVNGSVNGGNGTEERLDELRRLLGKSDGDLLKIVGIGAGAWGSVFAALLQDAYGRFREKV 65

Query: 68  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 127
           QIRIWRRAGR VD+ TAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDR LYADEIL+
Sbjct: 66  QIRIWRRAGRSVDRTTAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRTLYADEILR 125

Query: 128 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPII 187
           DGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERI+VP+I
Sbjct: 126 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERISVPVI 185

Query: 188 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 247
           ISLAKGIEA+L P+P IITPT MI+ ATGVP ENILYLGGPNIASEIYNKEYANARICG+
Sbjct: 186 ISLAKGIEASLDPIPRIITPTQMISSATGVPTENILYLGGPNIASEIYNKEYANARICGS 245

Query: 248 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 307
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 246 NKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 305

Query: 308 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 367
           HCTSEMIFIT+LL E+PEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELS DMGDSI GK
Sbjct: 306 HCTSEMIFITHLLTEQPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSPDMGDSIKGK 365

Query: 368 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 427
           GMIQG+SAV AF+E+LSQ SL+V HPE+NK VAP ELCPILK LY+ILIKR+ S + IL+
Sbjct: 366 GMIQGISAVGAFFELLSQPSLSVQHPEENKQVAPAELCPILKRLYRILIKRELSTRDILQ 425

Query: 428 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           ALRDET+NDPR+RIE+AQSHAFYRPSLLG+P
Sbjct: 426 ALRDETMNDPRERIEMAQSHAFYRPSLLGKP 456

BLAST of Moc01g16940 vs. ExPASy Swiss-Prot
Match: Q8S2G5 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0801600 PE=2 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.2e-221
Identity = 374/451 (82.93%), Postives = 414/451 (91.80%), Query Frame = 0

Query: 8   HSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 67
           H NG+T     +EEKL+ELRRLLGK+DGDPLRIVGVGAGAWGSVF AL+QD+YG  R+KV
Sbjct: 16  HGNGAT-----VEEKLDELRRLLGKADGDPLRIVGVGAGAWGSVFCALMQDAYGHLRDKV 75

Query: 68  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 127
           Q+RIWRR GR VD+ATAEHLFEVIN+REDVLRRLIRRCAYLKYVE RLGDR LYADEIL+
Sbjct: 76  QVRIWRRPGRAVDRATAEHLFEVINAREDVLRRLIRRCAYLKYVEGRLGDRTLYADEILR 135

Query: 128 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPII 187
           DGFCLNMIDTPLCPLKVV+NLQEAVWDADIV+NGLPST+TREVF EI +YWKERIT PII
Sbjct: 136 DGFCLNMIDTPLCPLKVVTNLQEAVWDADIVINGLPSTDTREVFGEIGRYWKERITAPII 195

Query: 188 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 247
           +SLAKGIEA+L P+P IITPT MI+ ATGVP+ENILYLGGPNIASEIYNKEYANARICGA
Sbjct: 196 LSLAKGIEASLDPLPRIITPTQMISNATGVPLENILYLGGPNIASEIYNKEYANARICGA 255

Query: 248 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 307
           +KWRKPLA FLRQP FIVWDNSDL+THEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 256 DKWRKPLAKFLRQPHFIVWDNSDLITHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 315

Query: 308 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 367
            CTSEMI+IT+LL EEPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGEL+L+MGDSI GK
Sbjct: 316 LCTSEMIYITHLLEEEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELTLEMGDSIKGK 375

Query: 368 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 427
           G IQGVSAV+AFYE+LSQ SL+V+HPE N+ VAPVE+CPILK LYKILIKR+  P +IL+
Sbjct: 376 GTIQGVSAVNAFYELLSQDSLSVMHPEANRSVAPVEMCPILKALYKILIKRELPPDSILQ 435

Query: 428 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           A+RDET+ DPR+RIE+AQ H+ YRPSLLGQP
Sbjct: 436 AIRDETMYDPRERIEMAQGHSLYRPSLLGQP 461

BLAST of Moc01g16940 vs. ExPASy Swiss-Prot
Match: Q9S785 (Glycerol-3-phosphate dehydrogenase [NAD(+)] At3g07690, cytosolic OS=Arabidopsis thaliana OX=3702 GN=At3g07690 PE=3 SV=2)

HSP 1 Score: 743.8 bits (1919), Expect = 1.2e-213
Identity = 355/439 (80.87%), Postives = 402/439 (91.57%), Query Frame = 0

Query: 19  LEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRV 78
           +EE+L+E+RR++GK+D DPLRIVGVGAGAWGSVF A+LQ++YG+FR KV +RIWRR GR 
Sbjct: 16  VEERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVSVRIWRRGGRA 75

Query: 79  VDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTP 138
           +DKATAEHLFEVINSRE++LRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMI+TP
Sbjct: 76  IDKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIETP 135

Query: 139 LCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIIISLAKGIEAAL 198
           LCPLKVV+NLQEAVWDADIV+NGLPSTET +VF EI KYWKER+  P+IISLAKG+EA  
Sbjct: 136 LCPLKVVTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEF 195

Query: 199 KPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFL 258
           +P P I+TPT MI+RATG+P+ENILYLGGPNIASE+YNKEYANARICG+EKWRKPL  FL
Sbjct: 196 EPHPRIVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFL 255

Query: 259 RQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITY 318
           RQ  FIVWDNSDL+THEVMGGLKNVYAIGAGMVA LT ESATSKSVYFAHCTSEMIFIT+
Sbjct: 256 RQSHFIVWDNSDLITHEVMGGLKNVYAIGAGMVATLTKESATSKSVYFAHCTSEMIFITH 315

Query: 319 LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVSA 378
           LLA+EPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGELSL+MGDSI GKGMIQGVSAV A
Sbjct: 316 LLAKEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELSLEMGDSIKGKGMIQGVSAVKA 375

Query: 379 FYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEALRDETLNDPR 438
           F+E+L+QSSL++ HPE+ KPV P ELCPILK LY+ILI R+ S +AILEALRDET+NDPR
Sbjct: 376 FFELLNQSSLSLQHPEEGKPVTPAELCPILKMLYRILITREFSCEAILEALRDETMNDPR 435

Query: 439 DRIELAQSHAFYRPSLLGQ 458
           + IE+A SH F++P LLGQ
Sbjct: 436 ELIEIAHSHLFFQPWLLGQ 454

BLAST of Moc01g16940 vs. ExPASy Swiss-Prot
Match: Q65X70 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0495700 PE=2 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 5.7e-213
Identity = 360/439 (82.00%), Postives = 400/439 (91.12%), Query Frame = 0

Query: 20  EEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRVV 79
           E KL+ELRR +GK+DGD LRIVGVG GAWGS F ALLQD+YG+ R+K Q+R+WRR GR V
Sbjct: 20  EGKLDELRRRMGKADGDLLRIVGVGGGAWGSAFCALLQDAYGRHRDKAQVRVWRRPGRAV 79

Query: 80  DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPL 139
           D+ATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDR LYADEIL+DGFCLNM+DTPL
Sbjct: 80  DRATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRTLYADEILRDGFCLNMVDTPL 139

Query: 140 CPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIIISLAKGIEAALK 199
           CPLKVV+NLQEAVWDADIV+NGLPSTETREVF EI +YWKERI  P+IISLAKGIEA++ 
Sbjct: 140 CPLKVVTNLQEAVWDADIVINGLPSTETREVFGEIGRYWKERIRPPVIISLAKGIEASID 199

Query: 200 PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFLR 259
           PVP IITPT MI+ ATGVP+ENILYLGGPNIASEIYNKEYANARICGA+KWRKPLA FLR
Sbjct: 200 PVPRIITPTQMISNATGVPLENILYLGGPNIASEIYNKEYANARICGADKWRKPLAKFLR 259

Query: 260 QPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITYL 319
           QP FIVWDNSDL+THEVMGGLKN+YAIGAGMVAALTNESATSKSVYF+ CTSEMI+IT+L
Sbjct: 260 QPHFIVWDNSDLITHEVMGGLKNIYAIGAGMVAALTNESATSKSVYFSLCTSEMIYITHL 319

Query: 320 LAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVSAF 379
           LAE+PEKLAGPLLADTYVTLLKGRNAWYGQ LAKGEL+L+MGDSI GKG IQGVSAV AF
Sbjct: 320 LAEDPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELTLEMGDSIKGKGTIQGVSAVHAF 379

Query: 380 YEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEALRDETLNDPRD 439
           YE+LSQSSL+V HPE  K VAPVELCPILKTLYKILIKR+ +  +IL+A+RDE++ DPR+
Sbjct: 380 YELLSQSSLSVTHPEVKKLVAPVELCPILKTLYKILIKRELATDSILQAIRDESMYDPRE 439

Query: 440 RIELAQSHAFYRPSLLGQP 459
           RIE++Q    YRPSLLG P
Sbjct: 440 RIEMSQRQCLYRPSLLGLP 458

BLAST of Moc01g16940 vs. ExPASy TrEMBL
Match: A0A6J1DI67 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LOC111021315 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 7.4e-264
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSY 60
           MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSY
Sbjct: 1   MSHHPLSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSY 60

Query: 61  GQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVL 120
           GQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVL
Sbjct: 61  GQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVL 120

Query: 121 YADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKE 180
           YADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKE
Sbjct: 121 YADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKE 180

Query: 181 RITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYA 240
           RITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYA
Sbjct: 181 RITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYA 240

Query: 241 NARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESAT 300
           NARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESAT
Sbjct: 241 NARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESAT 300

Query: 301 SKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDM 360
           SKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDM
Sbjct: 301 SKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDM 360

Query: 361 GDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDG 420
           GDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDG
Sbjct: 361 GDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDG 420

Query: 421 SPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           SPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 421 SPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 458

BLAST of Moc01g16940 vs. ExPASy TrEMBL
Match: A0A6J1HW44 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111467198 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 443/453 (97.79%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65
           LSHSNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 3   LSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 62

Query: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 63  KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 122

Query: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 123 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 182

Query: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245
           IIISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC
Sbjct: 183 IIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 242

Query: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305
           GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 243 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 302

Query: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 303 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 362

Query: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 363 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 422

Query: 426 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 423 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of Moc01g16940 vs. ExPASy TrEMBL
Match: A0A6J1HVE6 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111467198 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 443/453 (97.79%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65
           LSHSNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 40  LSHSNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 99

Query: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 100 KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 159

Query: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 160 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 219

Query: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245
           IIISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC
Sbjct: 220 IIISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 279

Query: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305
           GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 280 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 339

Query: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 340 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 399

Query: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 400 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 459

Query: 426 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 460 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 492

BLAST of Moc01g16940 vs. ExPASy TrEMBL
Match: A0A0A0KY21 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis sativus OX=3659 GN=Csa_4G340510 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 439/452 (97.12%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 7   SHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREK 66
           SH+NGSTHNCNGLE+KL+ELRRL+GKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREK
Sbjct: 4   SHTNGSTHNCNGLEDKLDELRRLIGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREK 63

Query: 67  VQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEIL 126
           VQIRIWRRAGRVVDK TAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEIL
Sbjct: 64  VQIRIWRRAGRVVDKVTAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEIL 123

Query: 127 KDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPI 186
           KDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETRE+FEEI KYWKERITVPI
Sbjct: 124 KDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREIFEEISKYWKERITVPI 183

Query: 187 IISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICG 246
           IISLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICG
Sbjct: 184 IISLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICG 243

Query: 247 AEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYF 306
           AEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYF
Sbjct: 244 AEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYF 303

Query: 307 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISG 366
           AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKG L+LDMGDSISG
Sbjct: 304 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGALNLDMGDSISG 363

Query: 367 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAIL 426
           KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDG+PQAIL
Sbjct: 364 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGTPQAIL 423

Query: 427 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           EALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 424 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of Moc01g16940 vs. ExPASy TrEMBL
Match: A0A6J1ELJ7 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC111435526 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.1e-254
Identity = 441/453 (97.35%), Postives = 448/453 (98.90%), Query Frame = 0

Query: 6   LSHSNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 65
           LSHSNGSTHN NG EEKL+ELRR+LGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE
Sbjct: 3   LSHSNGSTHNFNGFEEKLDELRRVLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFRE 62

Query: 66  KVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 125
           KVQIRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI
Sbjct: 63  KVQIRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEI 122

Query: 126 LKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVP 185
           LKDGFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVP
Sbjct: 123 LKDGFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVP 182

Query: 186 IIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARIC 245
           IIISLAKGIEAAL+PVPHIITPTLMINRAT VPIENILYLGGPNIASEIYNKEYANARIC
Sbjct: 183 IIISLAKGIEAALQPVPHIITPTLMINRATRVPIENILYLGGPNIASEIYNKEYANARIC 242

Query: 246 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 305
           GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY
Sbjct: 243 GAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVY 302

Query: 306 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 365
           FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS
Sbjct: 303 FAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSIS 362

Query: 366 GKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAI 425
           GKGMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAI
Sbjct: 363 GKGMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAI 422

Query: 426 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 423 LEALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of Moc01g16940 vs. TAIR 10
Match: AT2G41540.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 801.2 bits (2068), Expect = 4.3e-232
Identity = 396/451 (87.80%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 9   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 68
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 69  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 128
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 129 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPII 188
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 189 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 248
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 249 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 308
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 309 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 368
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 369 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 428
           GMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

Query: 429 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 ALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of Moc01g16940 vs. TAIR 10
Match: AT2G41540.2 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 801.2 bits (2068), Expect = 4.3e-232
Identity = 396/451 (87.80%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 9   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 68
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 69  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 128
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 129 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPII 188
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 189 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 248
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 249 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 308
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 309 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 368
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 369 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 428
           GMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

Query: 429 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 ALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of Moc01g16940 vs. TAIR 10
Match: AT2G41540.3 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 801.2 bits (2068), Expect = 4.3e-232
Identity = 396/451 (87.80%), Postives = 423/451 (93.79%), Query Frame = 0

Query: 9   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 68
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 69  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 128
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 129 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPII 188
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 189 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 248
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 249 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 308
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFA 311

Query: 309 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 368
           HCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISGK
Sbjct: 312 HCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISGK 371

Query: 369 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 428
           GMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL+
Sbjct: 372 GMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAILQ 431

Query: 429 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 459
           ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 ALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of Moc01g16940 vs. TAIR 10
Match: AT3G07690.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 735.7 bits (1898), Expect = 2.2e-212
Identity = 355/449 (79.06%), Postives = 402/449 (89.53%), Query Frame = 0

Query: 19  LEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRV 78
           +EE+L+E+RR++GK+D DPLRIVGVGAGAWGSVF A+LQ++YG+FR KV +RIWRR GR 
Sbjct: 16  VEERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVSVRIWRRGGRA 75

Query: 79  VDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTP 138
           +DKATAEHLFEVINSRE++LRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMI+TP
Sbjct: 76  IDKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIETP 135

Query: 139 LCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIIISLAKGIEAAL 198
           LCPLKVV+NLQEAVWDADIV+NGLPSTET +VF EI KYWKER+  P+IISLAKG+EA  
Sbjct: 136 LCPLKVVTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEF 195

Query: 199 KPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFL 258
           +P P I+TPT MI+RATG+P+ENILYLGGPNIASE+YNKEYANARICG+EKWRKPL  FL
Sbjct: 196 EPHPRIVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFL 255

Query: 259 RQPQFIVWDNSDLVTHEVMGGLKNVYAIGA----------GMVAALTNESATSKSVYFAH 318
           RQ  FIVWDNSDL+THEVMGGLKNVYAIGA          GMVA LT ESATSKSVYFAH
Sbjct: 256 RQSHFIVWDNSDLITHEVMGGLKNVYAIGAVFVLAFLYSTGMVATLTKESATSKSVYFAH 315

Query: 319 CTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKG 378
           CTSEMIFIT+LLA+EPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGELSL+MGDSI GKG
Sbjct: 316 CTSEMIFITHLLAKEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELSLEMGDSIKGKG 375

Query: 379 MIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEA 438
           MIQGVSAV AF+E+L+QSSL++ HPE+ KPV P ELCPILK LY+ILI R+ S +AILEA
Sbjct: 376 MIQGVSAVKAFFELLNQSSLSLQHPEEGKPVTPAELCPILKMLYRILITREFSCEAILEA 435

Query: 439 LRDETLNDPRDRIELAQSHAFYRPSLLGQ 458
           LRDET+NDPR+ IE+A SH F++P LLGQ
Sbjct: 436 LRDETMNDPRELIEIAHSHLFFQPWLLGQ 464

BLAST of Moc01g16940 vs. TAIR 10
Match: AT2G40690.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-07
Identity = 69/293 (23.55%), Postives = 125/293 (42.66%), Query Frame = 0

Query: 146 SNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIIISLAKGIEAALKPVPHII 205
           ++ + A+ DAD  ++ +P   +    E I  Y    +     ISL+KG+E     +   I
Sbjct: 151 TDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLP---FISLSKGLELNTLRMMSQI 210

Query: 206 TPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFLRQ----P 265
            P  + N     P +  + L GP+ A E+ N     A +  ++   K LAN ++Q     
Sbjct: 211 IPIALKN-----PRQPFVALSGPSFALELMN-NLPTAMVVASKD--KKLANAVQQLLASS 270

Query: 266 QFIVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSVYFAHCTSEMIFITYLLA 325
              +  +SD+   E+ G LKNV AI AG+V  + N    S +   +   SE+ ++   + 
Sbjct: 271 YLRINTSSDVTGVEIAGALKNVLAIAAGIVDGM-NLGNNSMAALVSQGCSEIRWLATKMG 330

Query: 326 EEPEKL-----AGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAV 385
            +P  +      G ++   +V L   RN   G  L  GE   D+  S++   + +GV+  
Sbjct: 331 AKPTTITGLSGTGDIMLTCFVNL--SRNRTVGVRLGSGETLDDILTSMN--QVAEGVATA 390

Query: 386 SAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEAL 430
            A   +  + ++ +               P+L  + KI+       +A+LE +
Sbjct: 391 GAVIALAQKYNVKL---------------PVLTAVAKIIDNELTPTKAVLELM 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153910.11.5e-263100.00probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic [Momordica cha... [more]
XP_038883653.11.4e-25698.23glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic [Benincasa hispida... [more]
XP_023544218.13.4e-25597.79probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cu... [more]
XP_022967787.13.4e-25597.79glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurb... [more]
XP_022967785.13.4e-25597.79probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cu... [more]
Match NameE-valueIdentityDescription
O222166.1e-23187.80Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic OS=Arabidopsis tha... [more]
Q8S0G43.3e-22986.47Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic OS=Oryza sativ... [more]
Q8S2G51.2e-22182.93Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic OS=Oryza sativ... [more]
Q9S7851.2e-21380.87Glycerol-3-phosphate dehydrogenase [NAD(+)] At3g07690, cytosolic OS=Arabidopsis ... [more]
Q65X705.7e-21382.00Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic OS=Oryza sativ... [more]
Match NameE-valueIdentityDescription
A0A6J1DI677.4e-264100.00Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1HW441.7e-25597.79Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HVE61.7e-25597.79Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0KY211.1e-25497.12Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A6J1ELJ73.1e-25497.35Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G41540.14.3e-23287.806-phosphogluconate dehydrogenase family protein [more]
AT2G41540.24.3e-23287.806-phosphogluconate dehydrogenase family protein [more]
AT2G41540.34.3e-23287.806-phosphogluconate dehydrogenase family protein [more]
AT3G07690.12.2e-21279.066-phosphogluconate dehydrogenase family protein [more]
AT2G40690.11.3e-0723.55NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPRINTSPR00077GPDHDRGNASEcoord: 42..59
score: 37.21
coord: 263..287
score: 40.73
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 7..457
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 270..431
e-value: 4.0E-33
score: 116.8
IPR006109Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminalPFAMPF07479NAD_Gly3P_dh_Ccoord: 270..424
e-value: 3.7E-24
score: 85.6
IPR011128Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminalPFAMPF01210NAD_Gly3P_dh_Ncoord: 142..244
e-value: 1.2E-10
score: 41.6
NoneNo IPR availableGENE3D3.40.50.720coord: 133..266
e-value: 6.3E-19
score: 70.4
NoneNo IPR availableGENE3D3.40.50.720coord: 20..101
e-value: 5.5E-7
score: 31.1
NoneNo IPR availablePIRSRPIRSR000114-3PIRSR000114-3coord: 135..417
e-value: 1.0E-32
score: 111.5
NoneNo IPR availablePANTHERPTHR11728:SF33GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]coord: 7..457
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 270..421
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 38..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g16940.1Moc01g16940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0046168 glycerol-3-phosphate catabolic process
biological_process GO:0006116 NADH oxidation
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0009331 glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0004367 glycerol-3-phosphate dehydrogenase [NAD+] activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor