MS028761 (gene) Bitter gourd (TR) v1

Overview
NameMS028761
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationscaffold155: 630982 .. 637297 (+)
RNA-Seq ExpressionMS028761
SyntenyMS028761
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTGGTTGGTTTGGGGAGTGCTGTTGGTCCTCCTCGGTGGCCATTCGTCTTCATCGTCTCCGGCGCCGGCGCCGGCGGCCTTCGTCGGAATCGGAGACGGCCATTTTCAGTTGAACGGTTCTCCATTTGTGTTCAATGGATTCAATTCTTATTGGATGATGTCCGTCGGAGCTGACCCAAACCAACGCCGGAAAGTCTCCGATGCCTTTCGCGACGCTGCCACCGCCGGCCTTACCGTCTGCCGGACTTGGGCCTTCGCCGACGGCGGCTATCACGCCCTCCAAATCTCTCCCGGCGTTTACGATGAATCGGTTTTTCAGGTTTTTCCTTTTCTTTTTCCTCTTTTAATCATATTAGAATTTTAAATATGTAATTTCATCATTTCACGTCAACTTCTGTATTAATATTTTTAATAATTTTTTTAACACTTTTTTTATTAATACATTTGCTCTTTCAACTTTTTCATGTCATGCTTATTTTATTAAATTCTCCTCCTTGAACTCTTAAGATTTATCGTATTTTATTCCTATACTTCAAAAGTTTCTTAATTTTAATAATGGGTTTACTATTTTTTATAGTACAATATATTGGGATATAAGAATTTGAATCTCTAACCTTAAGAAATTATTAGTAGTATCTGTTGAACTATGTTCATATTGACGTGTCTAGTCTTTCTTTATGAATTTCGTCGGTTTAACATTTATAATCTTTATGTTGATTACCTTGTATTATAGTTTTAATTATTACATTGTGAGATGATTCCAAATCAGTAGTTCGGATATTTGGTTGCATATATATGTAATTAAATTGACGAAGTTATAAAGACAAGAAAATTGAAATCCAAGACCTATCATAATTTTTTGGGAAATTATACTAAATGACATAATTAATATATATATAATTACTTTTATTTTTATTTTAAAATATGTCACAAAAATGATTTTTGGTTGGTCTATGTGATTATATGTTTTGTCATTTATATCATTTTTATAAAATTTTAAACTTCTATATATAAAAATACATATTCCATATTATTTTTGTTTTTTAAGATACATGAACTAAATAGATTCAATTTGGGTCGATTATATGTTTTGTCATTTATATGATTTTTGTAAAATTTTAAACTTCAATATATAAAAATACATATTCCGTGTTATTTTTGGTTTTTTAGGTACATGAACTAAATAGATTCAATGTCAAAGTTAAAGATTTATTAAATTTTTTTGTTTTTAAAATAATAGAGATTGAATAGATATAATCATAACTGAGATATCTTAATATTTTAATATTTTAACATTTTTAATTTTATCTATTTTTCGTATAAAAAAATTCAGGCGTCTATTTTTCCTTGAAACCCTAGGATTTCAAAACGTTTTCGGAATAAATTAGAACAGAAAGAAAGCAATTAATAAATAAACATGATTTTAAAAAATTTGAAATACTAAAAATAAAAACATTATCAAAGAGCTCTTATTTTTATGAAATAAAATAAAATAAAAGTTTAAATTCTATTTTAGTTTATACTTTTATATTTTGTCCCTAAATTTTTAAATATTTTGTGTTAACTTTCATAATTTTTTCTTTTAATCTCTTGACATTCAATTCTTTTATTTTTTATCTTTGAACTTTGCTTTTAAAAAGTACTACTTCAGTCCCTACCACTAATTTTCATTAATCGTTTAATAATAAACTAGACACAGTCTATTACACATATGTTTTAAACATTCGTCCATATAAATATCTATCGATCAAGTATATGCACAAAAAATGTGACAATATCATATTTCAGTTAAATATTAATTTATTAAAATATTAACAATTAATAATAAAATAGTTTTTTAATTAAAAGTTTAATAAGACAAAACATTACTAATCTATATAATCAACAATTACCTTCACCTGCTATTGCGAGTATAACTTAATGGTAATTGGCATATATCTCAAATCAAGAGGTGTGAGTTCAATCTCCCACACCCCACATATTGTACTAAAAAAATATGTCACCTTCACTCCGATTTGAACTCAAAAAAATTAAATATTTAAAAGTGGAAGAATAGAAAATGTTAAATATATGAAATGATACTTAATTTTTTCAATAATTCTCCATTTGATTCTCAATAATTTAAAATTCTCAAATTTACCTTTAATGTATGAAAATTCTCAATTAGGCCATTCCATTATTTTTTTTAACTTGTTAACAAAAATTTAATGATTTATTGTTTTATTAACATATTTGGACACATAAATATATTACTTGAGTGTTATTTGACACATGAAGTCCAACTTTCTCCTCAACAAAACTATATTCTTTGAACTCACTTGTCAGGTTAACAAAATAATAAATCTAATGTCGTATCAATTTTTCGTAAATAGATTTTAACATATTTTTCAATGAAATTACTCCATTGAGAATTCATAATGCAAGTAAAATTGAGGAGAAAATTTTTTCTAAATTTTGATAGAGTTATTGAAGGCTTAATTTCATTGAAATTAGTAAGTTTTTTGAGATCTTTACCTTACCTTTTGGGTGAATTAGCAAAAATGAAAGTTCTCTTTTTTTTCTCTCTCTTTTTTAAGAATTAAAAATAAAGATTTAAAATCAAAACGCCGTCGCTTTGATCAGGCTCTTGATTTTGTGATATGGGAGGCAAGAAAATACGGAATTTGGATAATATTGAGTTTGGTGAATAATTACGGCGACTACGGCGGAAGGCCGCAGTACGTCCGGTGGGCCCGGGCTGCCGGCGCTCAAATTCACAACGACGATGACTTCTATACAAATCAACTCATCAAGGGTTTCTACACGGGCCACGTCGAGGTAATCCCGTCTTTTAATTTCTATTTTGGTTTCTAAACTCTTAGAGTAAATTACATATTTTGAATATTGAGTTTGTTATTTAGTCTCTAAATTTTATAATGTTTCAATTCCGTTTGTAGAAAATTTTGAATCGGACTTATCTTTTGTCTCTACCGTCAATAATGGTTTGATAATTGGGTGGGTGTGACACTCGATCGAGTAATTAGTTTCATGGATTAGAAATTAATGGACAGAGTTAGATGTTTGGTTATTGATTTAGCAAATAACTGAGTTGAGTTGTATTTCACTCCTAACCACAACTTATCTGCTAATTTTCCAACATCATTAGTCAATTTGGTTATCCACTTAGTATCACCCAATCTTCATCCTGGTCATAGATAAGTCACCCAAATTCAAGTTTATAAGCAGAGTGCCAATTGGCCTCATGAAGATTCAATTTCACTGTGGCTAAGTCATTGTTTATGAGTCGTCAATTATTCTTCAACAAACATGTAGTCAGAGGTCCTGGTCTCCTCCTACTATTTAGAAGTGCTTACGGTTTCATGTTCATCGGCATGTAGGTCCTAAGAAAAGGTTTAGAATAATCAATTCGGTGATTCTATCAACCAATGTTGTTCATATCATGTATTAAAGATAAGTATGATTCTCCGAGTACCACTTCAAAAAATTATACCTTCCTTTTACTATCAGCAAAATAGGTAAGTTTGATTTATTAATTATTTCTAATCTAAGATCAACCTATCACTCCCATAGATTGTTAAGATTAATTTGAACATAGCTCAACAGGTTAAGATATTTATATCTGACTAAAAAGTCGTGAGTTCGAATTGATGTTGTACTTAAAAAAACATAGATCGTTAAATTGTTGCTTTTAAAATTTTGCAGAAAGTGTTGACAAGGAAGAACACAATGAGCGGCTTAAGGTATATGGATGATCCCACGATTATGGGATGGGAACTCATGAACGAACCACGTTGTGAAGTTGATTCCTCTGGCGCCACTGTTAATGTGAGCCCTACCATTTTATTATATATTTATTGCATCATCATTTTATTTTTAATTACCGTCATATTATACTTTCAGTTCATCATTCCTTAAGTCCCATAGGTTTGATCCTATAGAATCTGACCCATTTTTTCATTGAGCGAAAGGTACGAAATAAATCAGATTGATTTTTCGATCAAAAGTACTATGTGAATCTTCGTTTTTGTCCTCTTTCTCTATCCCTATCCCGCAGGTACAGTGTTTGAATCAATAGAGAACCTTTTGTTCTGTATCTGTATGAATCGCATTATTGCATTCCAATTCCTTCCCGATACCTCCCAAGGAAAATTCCAAATTGGATCCAAAATTGACGGGTTAGTGTGGTTTGGACTTTGATTTATTTTGGTTCCTTTCCTAAGTTTCCAAAACTAACACTATTGCTCCAGAAAACTTGAATTTAGTTTTCATTTAGTCCCTCGTATTTTAAATTGATACTTTTGGTCTTTGAGGTTTGGATTTAGTTTCTATTTAGTCCCTCCAATTTCAAAAATGGCATTTTTTGTTTTTCACATTTAGATATTATTTTTAGTTGGTCTTTGAAGTTTCAAAAGCAACACTTTTAGTCCTTAGAGTTCGGATTTTAATTTCTATTTAGTCCCTAACCCTAAGTTAAGTTAATAGATAGATGATTCAATAGTTTACTTCTTCTTGAACGTCTTCGATTGTTGAGAACTCTGACATACCCGTTGATAGTGACATATAAAAACCAAATCTAAACCTTAAAAGACCCAATAAAAGAAAATTTAAACCTTAGAGACCAAATAAAAAGAGTCCAAAAGTTTTAAGTGTAAAAACTTAAGAGATCAAATAGAATCTAAATTCAAACTTCAGTAATTAAAATGTTAACATGCTCCATTAATTACTAATTGGTTAGTATATTGACCAGTACAACCAATGTCTGATTGAAATCAATCCACTATTGGCTATATTCAATATGAATCAAATTTTAACTTGTATAAATTACTTGAATTCAATCATTCTTTTAGCTACTTTCTATTAATTTCATGAAGTTTCATCTCAAAATCAATTGATAATGAGAGATAGTCCATTTATATTATAAACTCTTATTAGATCCCTTGACATAGTATCTTTAACATGCCCCCTCGAGATGGTGCCTCGACCAATCTTGGTCGGATCCCCTTGAACTCTGATACCATATTAAGAATGAATAAACCGCTATTCAACCAAAAAGCTTAAGCCGGTGGATTTGGTAAATTCAATTCATTTTTGGATATCTAACTTCTCTAATTTGAGCGTGTTTGTGATGTGATAGAGATGGGTGGAAGAAATGGGAACTTATGTGAAGTCAATTGACAAAAAACATTTGGTGGGCATTGGAATGGAAGGATTCTACGGAGATTCAAACCCAAGTAGAATCAAATTCAACCCAAATGGTTACAAAGTTGGCACTGATTTTGTGACCAACAATCTCGTCAAAGTTATAGACTTTGCAACAATACATGCATATCCTGATTCTTGGTCAGTCCTTTCATTCTCTCTCTATATTTCTCCCTCTAAATTCTAATTTCTAATCACCTATTTGATCATTATTTTGTTTTTAAAAGTTGTGAAATGCTAATTATATTTCTTTGTTTTGAAATCTACACATTTTCCAAGTTTATTTAATAACAACTTGAGTTCTTATCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGATTTGTGAAAATAAAATCATATTTACCAATATAATTATATATTCTTAAATTATATTCTAAAAACAAAAACCTCATTTGATATTTGATATTTGATAACAATTATATTCTTAAATTTTCTATTTTATTATAAAGAATAAGCTCAAGTCATTTACTTGAACACTTTCGAATGAAATCGTAGTTGTTAGCCCAATTTCAAAAATTGAAACACAAACTTGTGATTTTATGTATTATTTCTTTTCGAGTTCTCAACATCTAGCTAAGAGAAAATTTAGTATAATCATTGAAAATAGAAAGCCGAAACATTATCAAGCCCAGAATAGAAAAATTACTATCAAACGGGACCTTCAAAATCGACCCTTGAAAAAGAAAGCATTGTAATTTGTTCTCCATTTTCACAGGCTACCAGGACAGAGTGAGGCAACCAAAATGGCATTCTTGGAGGAGTGGATGAAAATTCACTGGTCAGACTCAAAGACAATCTTAAAGAAGCCATTGATTTTTGCAGAATTTGGAAAGTCAACCAAAAACCAAAACCAAAACCAGGCATTCAGTGTAAGAGACAGAGATTCATTCCTCACTAAGGTTTATTCAAACATCTACAACCTTGCAAGAAATGGGACAACCATGGCTGGAGGTTTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCATATTACGATGGATACGAGATCGTTCTTTCCCAGAATCCTTCGACAACCGCCATTATAACGCAACAATCAAAAAACATGGCTGCCCTTCAACATTAA

mRNA sequence

ATGAGGTGGTTGGTTTGGGGAGTGCTGTTGGTCCTCCTCGGTGGCCATTCGTCTTCATCGTCTCCGGCGCCGGCGCCGGCGGCCTTCGTCGGAATCGGAGACGGCCATTTTCAGTTGAACGGTTCTCCATTTGTGTTCAATGGATTCAATTCTTATTGGATGATGTCCGTCGGAGCTGACCCAAACCAACGCCGGAAAGTCTCCGATGCCTTTCGCGACGCTGCCACCGCCGGCCTTACCGTCTGCCGGACTTGGGCCTTCGCCGACGGCGGCTATCACGCCCTCCAAATCTCTCCCGGCGTTTACGATGAATCGGTTTTTCAGGCAAGAAAATACGGAATTTGGATAATATTGAGTTTGGTGAATAATTACGGCGACTACGGCGGAAGGCCGCAGTACGTCCGGTGGGCCCGGGCTGCCGGCGCTCAAATTCACAACGACGATGACTTCTATACAAATCAACTCATCAAGGGTTTCTACACGGGCCACGTCGAGAAAGTGTTGACAAGGAAGAACACAATGAGCGGCTTAAGGTATATGGATGATCCCACGATTATGGGATGGGAACTCATGAACGAACCACGTTGTGAAGTTGATTCCTCTGGCGCCACTGTTAATAGATGGGTGGAAGAAATGGGAACTTATGTGAAGTCAATTGACAAAAAACATTTGGTGGGCATTGGAATGGAAGGATTCTACGGAGATTCAAACCCAAGTAGAATCAAATTCAACCCAAATGGTTACAAAGTTGGCACTGATTTTGTGACCAACAATCTCGTCAAAGTTATAGACTTTGCAACAATACATGCATATCCTGATTCTTGGCTACCAGGACAGAGTGAGGCAACCAAAATGGCATTCTTGGAGGAGTGGATGAAAATTCACTGGTCAGACTCAAAGACAATCTTAAAGAAGCCATTGATTTTTGCAGAATTTGGAAAGTCAACCAAAAACCAAAACCAAAACCAGGCATTCAGTGTAAGAGACAGAGATTCATTCCTCACTAAGGTTTATTCAAACATCTACAACCTTGCAAGAAATGGGACAACCATGGCTGGAGGTTTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCATATTACGATGGATACGAGATCGTTCTTTCCCAGAATCCTTCGACAACCGCCATTATAACGCAACAATCAAAAAACATGGCTGCCCTTCAACATTAA

Coding sequence (CDS)

ATGAGGTGGTTGGTTTGGGGAGTGCTGTTGGTCCTCCTCGGTGGCCATTCGTCTTCATCGTCTCCGGCGCCGGCGCCGGCGGCCTTCGTCGGAATCGGAGACGGCCATTTTCAGTTGAACGGTTCTCCATTTGTGTTCAATGGATTCAATTCTTATTGGATGATGTCCGTCGGAGCTGACCCAAACCAACGCCGGAAAGTCTCCGATGCCTTTCGCGACGCTGCCACCGCCGGCCTTACCGTCTGCCGGACTTGGGCCTTCGCCGACGGCGGCTATCACGCCCTCCAAATCTCTCCCGGCGTTTACGATGAATCGGTTTTTCAGGCAAGAAAATACGGAATTTGGATAATATTGAGTTTGGTGAATAATTACGGCGACTACGGCGGAAGGCCGCAGTACGTCCGGTGGGCCCGGGCTGCCGGCGCTCAAATTCACAACGACGATGACTTCTATACAAATCAACTCATCAAGGGTTTCTACACGGGCCACGTCGAGAAAGTGTTGACAAGGAAGAACACAATGAGCGGCTTAAGGTATATGGATGATCCCACGATTATGGGATGGGAACTCATGAACGAACCACGTTGTGAAGTTGATTCCTCTGGCGCCACTGTTAATAGATGGGTGGAAGAAATGGGAACTTATGTGAAGTCAATTGACAAAAAACATTTGGTGGGCATTGGAATGGAAGGATTCTACGGAGATTCAAACCCAAGTAGAATCAAATTCAACCCAAATGGTTACAAAGTTGGCACTGATTTTGTGACCAACAATCTCGTCAAAGTTATAGACTTTGCAACAATACATGCATATCCTGATTCTTGGCTACCAGGACAGAGTGAGGCAACCAAAATGGCATTCTTGGAGGAGTGGATGAAAATTCACTGGTCAGACTCAAAGACAATCTTAAAGAAGCCATTGATTTTTGCAGAATTTGGAAAGTCAACCAAAAACCAAAACCAAAACCAGGCATTCAGTGTAAGAGACAGAGATTCATTCCTCACTAAGGTTTATTCAAACATCTACAACCTTGCAAGAAATGGGACAACCATGGCTGGAGGTTTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCATATTACGATGGATACGAGATCGTTCTTTCCCAGAATCCTTCGACAACCGCCATTATAACGCAACAATCAAAAAACATGGCTGCCCTTCAACATTAA

Protein sequence

MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQARKYGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQH
Homology
BLAST of MS028761 vs. NCBI nr
Match: XP_022141751.1 (mannan endo-1,4-beta-mannosidase 5-like [Momordica charantia])

HSP 1 Score: 815.1 bits (2104), Expect = 2.7e-232
Identity = 393/405 (97.04%), Postives = 394/405 (97.28%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD
Sbjct: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQRRKVSDAFRDAA AGLTVCRTWAFADGGYHALQISPGVYDESVFQ        ARKY
Sbjct: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN
Sbjct: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF
Sbjct: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDSNPSRIKFNPNGYKVGTDFVTNNL+KVIDFATIHAYPDSWLPGQSEATKMAFLEEWM
Sbjct: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
           KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA
Sbjct: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQH 398
           GGLVWQVMAEGMESYYDGYEIVLSQNP TTAII QQSKNMAALQH
Sbjct: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 405

BLAST of MS028761 vs. NCBI nr
Match: XP_038890634.1 (mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida])

HSP 1 Score: 659.8 bits (1701), Expect = 1.4e-185
Identity = 319/403 (79.16%), Postives = 350/403 (86.85%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWL+W V  +L G    SSSP+PAP AFVG+ DGHFQLNGSPFVFNGFNSYWMMSV AD
Sbjct: 7   MRWLIWEVFFILSG---LSSSPSPAPEAFVGVQDGHFQLNGSPFVFNGFNSYWMMSVAAD 66

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KVS+ F DAA+A LTVCRTWAFADGG  ALQISPGVYDE VFQ        A+KY
Sbjct: 67  PNQRHKVSNVFHDAASAALTVCRTWAFADGGSPALQISPGVYDEIVFQGLDFVICEAKKY 126

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNNY DYGGR QYVRWA+AAG  I+NDDDFYTNQLIK +Y  HV++VLTRKN
Sbjct: 127 GIRMILSLVNNYKDYGGREQYVRWAKAAGVDIYNDDDFYTNQLIKSYYKNHVQRVLTRKN 186

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           T+SGLRYMDDPTIMGWELMNEPRC++DSSG T+NRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 187 TISGLRYMDDPTIMGWELMNEPRCQLDSSGNTINRWVEEMGSYVKSIDKQHLVGIGMEGF 246

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
            GDS+P++IK NPN Y++GTDF+TNNL K IDFATIHAYPD+WLPGQSEATKMAFLEEW+
Sbjct: 247 CGDSSPTKIKSNPNSYRLGTDFLTNNLNKDIDFATIHAYPDAWLPGQSEATKMAFLEEWI 306

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            IHW+DSKTILKKPLIF EFGKST+ QNQ    +VRDRDSFL+KVYS IYNLARNG TMA
Sbjct: 307 TIHWTDSKTILKKPLIFEEFGKSTRGQNQTS--NVRDRDSFLSKVYSIIYNLARNGATMA 366

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAAL 396
           GGLVWQVMAEGMESYYDGYEIVL QNPST AIITQQS  MAAL
Sbjct: 367 GGLVWQVMAEGMESYYDGYEIVLPQNPSTNAIITQQSNKMAAL 404

BLAST of MS028761 vs. NCBI nr
Match: XP_008459570.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 652.9 bits (1683), Expect = 1.8e-183
Identity = 312/404 (77.23%), Postives = 351/404 (86.88%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KVS  FRDAATAGLTVCRTWAF DGG+HALQISPGVYDESVFQ        ARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQ 397
           GGLVWQVMAEGMESYYDGYEIVLSQNPST AIITQQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 406

BLAST of MS028761 vs. NCBI nr
Match: XP_004141536.2 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical protein Csa_008142 [Cucumis sativus])

HSP 1 Score: 648.3 bits (1671), Expect = 4.4e-182
Identity = 309/403 (76.67%), Postives = 348/403 (86.35%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWL+W +  ++     S+SS +PAPAAFVG+ DGHFQLNGSPF+FNGFNSYWMMSV  D
Sbjct: 9   MRWLIWALFFLI--STLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATD 68

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KV+  FRDAA AGLTVCRTWAF DGG+HALQISPGVYDESVFQ        ARKY
Sbjct: 69  PNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 128

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+H++DDFYTNQLIK +Y  HV+KVL+RKN
Sbjct: 129 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKN 188

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TM+GL YM+D TIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 189 TMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGF 248

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS+P++IK NP  +K GTDF+TNNL K IDFATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 249 YGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWI 308

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFGKS ++QNQ  + SVRDRD+FL+KV+S IYNLARNG TMA
Sbjct: 309 ALHWTDSKTILKKPLIFEEFGKSIRDQNQTSS-SVRDRDAFLSKVFSIIYNLARNGATMA 368

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAAL 396
           GGLVWQVMAEGMESYYDGYEIVLSQ PSTTAIIT QS NMAAL
Sbjct: 369 GGLVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAAL 408

BLAST of MS028761 vs. NCBI nr
Match: XP_004141631.3 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KAE8648995.1 hypothetical protein Csa_008431 [Cucumis sativus])

HSP 1 Score: 631.3 bits (1627), Expect = 5.5e-177
Identity = 302/403 (74.94%), Postives = 339/403 (84.12%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRW +WG+  ++    + SSS +PAPAAFVG+ D HFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   MRWSIWGIFFLI---STLSSSSSPAPAAFVGVQDDHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KV+  FRDAATAGLTVCRTWAF DG  HALQISPGVYDESVFQ        ARKY
Sbjct: 68  PNQRHKVTQVFRDAATAGLTVCRTWAFNDGALHALQISPGVYDESVFQALDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI ++LSLVNN+ +YGGR  YVRWA AAG Q+H+DDDFYTNQLI+ +Y  +V+KVL RKN
Sbjct: 128 GIRMLLSLVNNFEEYGGRAAYVRWAEAAGVQVHSDDDFYTNQLIRTYYKNYVKKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TM+GLRYM+D TIMGWELMNE RC+VDSSG T+NRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 188 TMNGLRYMEDATIMGWELMNEARCQVDSSGNTINRWVEEMGSYVKSIDKQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS+P++IK NP  +K GTDFVTNNL K IDFATIH YPD+WLPG+SEAT+MAFLEEWM
Sbjct: 248 YGDSSPNKIKANPGSFKFGTDFVTNNLNKAIDFATIHVYPDAWLPGKSEATRMAFLEEWM 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW DSK ILKKPLI  EFGKS +   QNQ FSVRD D+FL+KVYS IYNLAR G TMA
Sbjct: 308 ALHWMDSKNILKKPLILEEFGKSIR--GQNQTFSVRDSDAFLSKVYSIIYNLARKGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAAL 396
           GGLVWQVMAEGMESYYDGYEIVLSQNPST  IIT+QS  MAAL
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNTIITKQSNKMAAL 405

BLAST of MS028761 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 491.5 bits (1264), Expect = 8.9e-138
Identity = 228/377 (60.48%), Postives = 290/377 (76.92%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRTWAFA 88
           FV +   HF+LNGSPF+FNGFNSYW+M V A+P++R KVS+  R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 89  DGGYHALQISPGVYDESVFQ--------ARKYGIWIILSLVNNYGDYGGRPQYVRWARAA 148
           DGG  ALQISPGVYDE VFQ        A+KYGI +ILS VNNY D+GG+ QYV+WAR A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 149 GAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDS 208
           GAQI+ DDDFYTN + K +Y  H++KV+TR NT++G+ Y DD TIM WELMNEPR + D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 209 SGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLV 268
           SG T+N WV+EM ++VKS+D KHL+ IGMEGFYGDS P R   NP GY+VGTDF++N+L+
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINP-GYQVGTDFISNHLI 278

Query: 269 KVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQN 328
           K IDFATIHAY D WL GQS+  +M F+++WM  HW D+K ILKKPL+ AEFGKS+++  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 329 QNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPS 388
            NQ      RD+F++ +Y NIY+LA++G TM G L+WQ++A+GME+Y DGY I L +NPS
Sbjct: 339 YNQNI----RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPS 398

Query: 389 TTAIITQQSKNMAALQH 398
           T  IIT QS  M AL H
Sbjct: 399 TAGIITSQSHAMTALAH 410

BLAST of MS028761 vs. ExPASy Swiss-Prot
Match: Q6YM50 (Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 8.9e-138
Identity = 228/377 (60.48%), Postives = 290/377 (76.92%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRTWAFA 88
           FV +   HF+LNGSPF+FNGFNSYW+M V A+P++R KVS+  R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 89  DGGYHALQISPGVYDESVFQ--------ARKYGIWIILSLVNNYGDYGGRPQYVRWARAA 148
           DGG  ALQISPGVYDE VFQ        A+KYGI +ILS VNNY D+GG+ QYV+WAR A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 149 GAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDS 208
           GAQI+ DDDFYTN + K +Y  H++KV+TR NT++G+ Y DD TIM WELMNEPR + D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 209 SGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLV 268
           SG T+N WV+EM ++VKS+D KHL+ IGMEGFYGDS P R   NP GY+VGTDF++N+L+
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINP-GYQVGTDFISNHLI 278

Query: 269 KVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQN 328
           K IDFATIHAY D WL GQS+  +M F+++WM  HW D+K ILKKPL+ AEFGKS+++  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 329 QNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPS 388
            NQ      RD+F++ +Y NIY+LA++G TM G L+WQ++A+GME+Y DGY I L +NPS
Sbjct: 339 YNQNI----RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPS 398

Query: 389 TTAIITQQSKNMAALQH 398
           T  IIT QS  M AL H
Sbjct: 399 TAGIITSQSHAMTALAH 410

BLAST of MS028761 vs. ExPASy Swiss-Prot
Match: O48540 (Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 SV=2)

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-133
Identity = 222/379 (58.58%), Postives = 285/379 (75.20%), Query Frame = 0

Query: 25  APAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRT 84
           A + F+G+ D HF+LNGSPF+FNGFNSYW+M V ADP +R KV++  +DA+ AGL+VCRT
Sbjct: 23  ANSGFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADPTERYKVTEVLKDASVAGLSVCRT 82

Query: 85  WAFADGGYHALQISPGVYDESVFQ--------ARKYGIWIILSLVNNYGDYGGRPQYVRW 144
           WAF+DGG  ALQISPG+YDE VFQ        A+KYGI +ILS VN + D+GG+ QYV W
Sbjct: 83  WAFSDGGDRALQISPGIYDERVFQGLDFVIAEAKKYGIRLILSFVNQWNDFGGKAQYVWW 142

Query: 145 ARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRC 204
           AR AGAQI NDD+FYT+ ++K +   H+EKV+TR N+++ + Y DDPTIM WELMNEPR 
Sbjct: 143 ARNAGAQISNDDEFYTHPMLKKYLKNHIEKVVTRLNSITKVAYKDDPTIMAWELMNEPRD 202

Query: 205 EVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVT 264
           + D SG TVN WV+EM ++VKS+D KHL+ +GMEGFYGDS P R   NP GY+VGTDF++
Sbjct: 203 QADYSGKTVNGWVQEMASFVKSLDNKHLLEVGMEGFYGDSIPERKSVNP-GYQVGTDFIS 262

Query: 265 NNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKST 324
           N+L+  IDFATIHAY D W+ GQS+  ++ ++E+W+  HW D++ ILKKPL+ AEFGKS+
Sbjct: 263 NHLINEIDFATIHAYTDQWVSGQSDDAQLVWMEKWITSHWEDARNILKKPLVLAEFGKSS 322

Query: 325 KNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLS 384
           + Q          RD F++ VY N+YNLA+ G TMAG LVWQ+MA GME+Y DGY IVL 
Sbjct: 323 RGQG--------SRDIFMSSVYRNVYNLAKEGGTMAGSLVWQLMAHGMENYDDGYCIVLG 382

Query: 385 QNPSTTAIITQQSKNMAAL 396
           Q PSTT II+ Q+  M AL
Sbjct: 383 QTPSTTQIISDQAHVMTAL 392

BLAST of MS028761 vs. ExPASy Swiss-Prot
Match: Q9FUQ6 (Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.2e-129
Identity = 225/405 (55.56%), Postives = 295/405 (72.84%), Query Frame = 0

Query: 2   RWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADP 61
           R  + G+ L+LL      +    A + F+G+ D HF+LNGSPF+FNGFNSYW+M V ADP
Sbjct: 7   RSCISGLFLLLL------ALSCEANSGFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADP 66

Query: 62  NQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKYG 121
            +R KV++  +DA+TAGL+VCRTWAF+DGG  ALQISPGVYDE VFQ        A+KYG
Sbjct: 67  AERYKVTEVLKDASTAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVIAEAKKYG 126

Query: 122 IWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNT 181
           + +ILS VN + D+GG+ +YV WAR AG QI NDD+FYT+ ++K +   H+E V+TR N+
Sbjct: 127 VRLILSFVNQWNDFGGKAEYVWWARNAGVQISNDDEFYTHPILKKYLKNHIE-VVTRLNS 186

Query: 182 MSGLRYMDDPTIMGWELMNEPRCEVDSSGATVN-RWVEEMGTYVKSIDKKHLVGIGMEGF 241
           ++ + Y DD TIM WELMNEPR + D SG TVN  WV+EM ++VKS+D KHL+ +GMEGF
Sbjct: 187 ITKVAYKDDATIMAWELMNEPRDQADYSGKTVNVGWVQEMASFVKSLDNKHLLEVGMEGF 246

Query: 242 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 301
           YGDS P R   NP GY+VGTDF++N+L+  IDFATIHAY D WL GQS+  ++A++E+W+
Sbjct: 247 YGDSIPERKLVNP-GYQVGTDFISNHLINEIDFATIHAYTDQWLSGQSDEAQLAWMEKWI 306

Query: 302 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 361
           + HW D++ ILKKPL+ AEFGKS+++   +       RD F++ VY N+YNLA+ G TM 
Sbjct: 307 RSHWEDARNILKKPLVLAEFGKSSRSGEGS-------RDIFMSSVYRNVYNLAKEGGTMG 366

Query: 362 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQH 398
           G LVWQ+MA GME+Y DGY IVL  NPSTT II+ Q+  M AL H
Sbjct: 367 GSLVWQLMAHGMENYDDGYSIVLGLNPSTTQIISNQAHIMTALAH 396

BLAST of MS028761 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-116
Identity = 199/377 (52.79%), Postives = 265/377 (70.29%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRTWAFA 88
           FV      F L+G+P+  NGFN+YW+M + ADP+QR KVS A  +AA  GLTV RTWAF+
Sbjct: 37  FVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALGEAAGHGLTVARTWAFS 96

Query: 89  D-GGYHALQISPGVYDESVF--------QARKYGIWIILSLVNNYGDYGGRPQYVRWARA 148
           D GG +ALQ+SPG Y+E+ F        +ARKYGI +ILSLV+NY  +GGR QYV WARA
Sbjct: 97  DGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDNYDSFGGRKQYVNWARA 156

Query: 149 AGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVD 208
            G  I +DD+F+TN ++KGFY  HV+ VLTRKNT++G+ Y DDPTI+ WELMNEPRC+ D
Sbjct: 157 QGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITGVAYRDDPTILAWELMNEPRCQSD 216

Query: 209 SSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRI-KFNPNGYKVGTDFVTNN 268
            SG TV  W+ EM  +VKSID+ H++ +G+EGFYG S+PSRI   NP+GY++GTDF+ NN
Sbjct: 217 LSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGASSPSRIAAVNPSGYQLGTDFIANN 276

Query: 269 LVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKN 328
            V  IDFAT+H+YPD WL G+ +  ++ F+  W+  H +D++ +L+KPL+ AEFGKS K 
Sbjct: 277 QVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAHIADAQAVLRKPLLIAEFGKSWK- 336

Query: 329 QNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQN 388
              +  +S   RD+    VY+ IY  AR G    GGL WQ++  GM+SY DGYE+V  + 
Sbjct: 337 ---DPGYSSGQRDALYGTVYAKIYESARRGGATVGGLFWQLLVPGMDSYRDGYEVVFGET 396

Query: 389 PSTTAIITQQSKNMAAL 396
           PSTT +IT  S+ +  L
Sbjct: 397 PSTTGVITTNSRRLRFL 409

BLAST of MS028761 vs. ExPASy TrEMBL
Match: A0A6J1CK63 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.3e-232
Identity = 393/405 (97.04%), Postives = 394/405 (97.28%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD
Sbjct: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQRRKVSDAFRDAA AGLTVCRTWAFADGGYHALQISPGVYDESVFQ        ARKY
Sbjct: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN
Sbjct: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF
Sbjct: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDSNPSRIKFNPNGYKVGTDFVTNNL+KVIDFATIHAYPDSWLPGQSEATKMAFLEEWM
Sbjct: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
           KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA
Sbjct: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQH 398
           GGLVWQVMAEGMESYYDGYEIVLSQNP TTAII QQSKNMAALQH
Sbjct: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 405

BLAST of MS028761 vs. ExPASy TrEMBL
Match: A0A5D3BKZ3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00890 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.6e-184
Identity = 312/404 (77.23%), Postives = 351/404 (86.88%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KVS  FRDAATAGLTVCRTWAF DGG+HALQISPGVYDESVFQ        ARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQ 397
           GGLVWQVMAEGMESYYDGYEIVLSQNPST AIITQQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 406

BLAST of MS028761 vs. ExPASy TrEMBL
Match: A0A1S3CB04 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.6e-184
Identity = 312/404 (77.23%), Postives = 351/404 (86.88%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KVS  FRDAATAGLTVCRTWAF DGG+HALQISPGVYDESVFQ        ARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQ 397
           GGLVWQVMAEGMESYYDGYEIVLSQNPST AIITQQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 406

BLAST of MS028761 vs. ExPASy TrEMBL
Match: A0A0A0KSS2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.1e-182
Identity = 309/403 (76.67%), Postives = 348/403 (86.35%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWL+W +  ++     S+SS +PAPAAFVG+ DGHFQLNGSPF+FNGFNSYWMMSV  D
Sbjct: 9   MRWLIWALFFLI--STLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATD 68

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------ARKY 120
           PNQR KV+  FRDAA AGLTVCRTWAF DGG+HALQISPGVYDESVFQ        ARKY
Sbjct: 69  PNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 128

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+H++DDFYTNQLIK +Y  HV+KVL+RKN
Sbjct: 129 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKN 188

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TM+GL YM+D TIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 189 TMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGF 248

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS+P++IK NP  +K GTDF+TNNL K IDFATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 249 YGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWI 308

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFGKS ++QNQ  + SVRDRD+FL+KV+S IYNLARNG TMA
Sbjct: 309 ALHWTDSKTILKKPLIFEEFGKSIRDQNQTSS-SVRDRDAFLSKVFSIIYNLARNGATMA 368

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAAL 396
           GGLVWQVMAEGMESYYDGYEIVLSQ PSTTAIIT QS NMAAL
Sbjct: 369 GGLVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAAL 408

BLAST of MS028761 vs. ExPASy TrEMBL
Match: A0A5A7TCI2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00910 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.8e-166
Identity = 288/396 (72.73%), Postives = 328/396 (82.83%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQARKYGIWIILSL 120
           PNQR KVS  FRDAATAGLT                      D  +F+ARKYGI +ILSL
Sbjct: 68  PNQRHKVSQVFRDAATAGLT--------------------GLDFVIFEARKYGIRMILSL 127

Query: 121 VNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYM 180
           VNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKNTMS LRYM
Sbjct: 128 VNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKNTMSCLRYM 187

Query: 181 DDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSR 240
           DDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGFYGDS P++
Sbjct: 188 DDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGFYGDSIPTK 247

Query: 241 IKFNPNGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSK 300
           IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+ +HW+DSK
Sbjct: 248 IKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWVALHWTDSK 307

Query: 301 TILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVM 360
           TILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMAGGLVWQVM
Sbjct: 308 TILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMAGGLVWQVM 367

Query: 361 AEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQ 397
           AEGMESYYDGYEIVLSQNPST AIITQQS  MAAL+
Sbjct: 368 AEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 378

BLAST of MS028761 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 8.7e-112
Identity = 187/376 (49.73%), Postives = 248/376 (65.96%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRTWAFA 88
           FV      F LNG P+  NGFN+YW+M V +DP+QR K+S AF+DA+  GLTV RTWAF+
Sbjct: 31  FVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFS 90

Query: 89  DGGYHALQISPGVYDESVFQ--------ARKYGIWIILSLVNNYGDYGGRPQYVRWARAA 148
           DGGY ALQ SPG Y+E +FQ        AR++GI IILS  NNY  +GGR QYV WAR+ 
Sbjct: 91  DGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSR 150

Query: 149 GAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDS 208
           G  + ++DDF+T+ L+K FY  H++ VL R NT + + Y DDPTIM WELMNEPRC  D 
Sbjct: 151 GRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDP 210

Query: 209 SGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLV 268
           SG  +  W+ EM  +VKS+D+ HL+  G+EGFYG S+P     NP G + GTDF+ NN +
Sbjct: 211 SGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPG-QFGTDFIANNRI 270

Query: 269 KVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQN 328
             IDF T+H+YPD W P  SE ++M FL +W+  H  D++ +L KP+I AEFGKS K   
Sbjct: 271 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 330

Query: 329 QNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPS 388
              A     RD     VYS IY  A+ G   AGGL WQ++  G++++ DGY I+LSQ+ S
Sbjct: 331 YTPA----QRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSS 390

Query: 389 TTAIITQQSKNMAALQ 397
           T  +I+QQS+ +  ++
Sbjct: 391 TVNVISQQSRKLTLIR 401

BLAST of MS028761 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.9e-104
Identity = 184/408 (45.10%), Postives = 251/408 (61.52%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAA--FVGIGDGHFQLNGSPFVFNGFNSYWMMSVG 60
           M+ L + VLL +L   +SS     + ++  FV      F LNG PF  NGFN+YW+    
Sbjct: 1   MKCLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEA 60

Query: 61  ADPNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQISPGVYDESVFQ--------AR 120
            D   R K++  F++A    LT+ RTW F DGGY ALQI+PGVYDE  FQ        A+
Sbjct: 61  TDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAK 120

Query: 121 KYGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTR 180
           + GI +I++ VNNY D+GGR QYV WA+  G  + +DDDFYTN L+K +Y  HV+ ++ R
Sbjct: 121 RLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNR 180

Query: 181 KNTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGME 240
            NT + + Y D+PTIMGWELMNEP+C  D SG T+  W+ EM  YVKS+D KHL+  G+E
Sbjct: 181 VNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLE 240

Query: 241 GFYGDSNPSR-IKFNPNGYKV-GTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFL 300
           GFYGDS+P R    NP    V GTDF+ N+ +  IDFA+IH+YPD W P   E +++  L
Sbjct: 241 GFYGDSSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 300

Query: 301 EEWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNG 360
            +W++ H  D++ ILKKPLI  EFGK T      QA     RD+     +  IY  A  G
Sbjct: 301 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQA----QRDAVFNATFDTIYESAEKG 360

Query: 361 TTMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAALQ 397
              AG L W V+++GM ++ D   IVLS+N +T  IIT++S+ +  ++
Sbjct: 361 GPAAGALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIR 404

BLAST of MS028761 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 1.6e-102
Identity = 180/367 (49.05%), Postives = 234/367 (63.76%), Query Frame = 0

Query: 37  FQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAATAGLTVCRTWAFADGGYHALQ 96
           F LNG PF  NGFN+YWMM++ AD + R KV++ F+ A+  G+TV RTWAF DG + ALQ
Sbjct: 55  FTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQ 114

Query: 97  ISPGVYDESVF--------QARKYGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDD 156
            SP VYDE VF        +ARKY I +ILSLVNN+  YGG+ QYV+W  A+G  + +DD
Sbjct: 115 KSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASGLNLTSDD 174

Query: 157 DFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRW 216
           DF+TN  ++ FY  HV  VL R NT + + Y +DPTI  WELMNEPRC  D SG  +  W
Sbjct: 175 DFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSW 234

Query: 217 VEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGY--KVGTDFVTNNLVKVIDFA 276
           ++EM  +VKS+D KHLV IG+EGFYG S P+R +FNPN Y  +VGTDF+ NN V  IDFA
Sbjct: 235 IQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFA 294

Query: 277 TIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFS 336
           ++H YPDSW+      + + F   WM+ H  D++  L  P++F EFG S  +   N +F 
Sbjct: 295 SVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSF- 354

Query: 337 VRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIIT 394
              RD  L  VY    N  R G   AG LVWQV  +G E   DGY + L++  + + II+
Sbjct: 355 ---RDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASKIIS 414

BLAST of MS028761 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 3.3e-95
Identity = 179/383 (46.74%), Postives = 231/383 (60.31%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD--PNQRRKVSDAFRDAATAGLTVCRTWA 88
           FVG     F LNG     NGFN+YWMM+  AD     R  V+ A R A+  G+ V R W 
Sbjct: 29  FVGRNGTQFVLNGEQVYLNGFNAYWMMTTAADTASKGRATVTTALRQASAVGMNVARIWG 88

Query: 89  FADGGYHALQISPGVYDESVF--------QARKYGIWIILSLVNNYGDYGGRPQYVRWAR 148
           F +G Y  LQISPG Y E VF        +A ++ I +I+SLVNN+ DYGGR +YV W  
Sbjct: 89  FNEGDYIPLQISPGSYSEDVFKGLDFVVYEAGRFNIKLIISLVNNFEDYGGRKKYVEW-- 148

Query: 149 AAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEV 208
              A +   D+FYTN  +K FY  HV+ VLTRKNT++G  Y DDPTI  WEL+NEPRC  
Sbjct: 149 ---AGLDEPDEFYTNSAVKQFYKNHVKTVLTRKNTITGRMYKDDPTIFSWELINEPRCND 208

Query: 209 DSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNG-YKVGTDFVTN 268
            ++   +  WV+EM +YVKSID  HL+ IG+EGFYG+S P R  +NP G    GTDF+TN
Sbjct: 209 STASNILQDWVKEMASYVKSIDSNHLLEIGLEGFYGESIPERTVYNPGGRVLTGTDFITN 268

Query: 269 NLVKVIDFATIHAYPDSWLPGQSEAT--KMAFLEEWMKIHWSDSKTILKKPLIFAEFGKS 328
           N +  IDFATIH YPDSWLP QS  T  +  F++ W+  H  D   I+KKPL+  EFGKS
Sbjct: 269 NQIPDIDFATIHIYPDSWLPLQSSRTGEQDTFVDRWIGAHIEDCDNIIKKPLLITEFGKS 328

Query: 329 TKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVL 388
           +K       FS+  R+ F  +VY  IY+ AR G +  GG+ WQ+         DGYE+ +
Sbjct: 329 SK----YPGFSLEKRNKFFQRVYDVIYDSARAGGSCTGGVFWQLTTNRTGLLGDGYEVFM 388

Query: 389 SQNPSTTA-IITQQSKNMAALQH 398
              P+TTA +I  QS  +  L++
Sbjct: 389 QAGPNTTAQLIADQSSKLKNLKY 402

BLAST of MS028761 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.1e-94
Identity = 173/406 (42.61%), Postives = 243/406 (59.85%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           M+ L + VLL ++   S     A     FV      F LNG PF  NGFN+YW+     D
Sbjct: 1   MKCLCFIVLLAIVIAQSYVGVEAAPSDGFVSRNGVQFILNGKPFYANGFNAYWLAYEATD 60

Query: 61  PNQRRKVSDAFRDAATAGLTVCRTWAFADGG-YHALQISPGVYDESVFQ--------ARK 120
           P  R K+++ F++A + GLT+ RTW F DG  Y ALQ +PG YDE  FQ        A++
Sbjct: 61  PTTRFKITNVFQNATSLGLTIARTWGFRDGAIYRALQTAPGSYDEQTFQGLDFVIAEAKR 120

Query: 121 YGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRK 180
            GI +I+ LVNN+ DYGG+ QYV WAR+ G  + ++DDFY N +IK FY  HV+ VL R 
Sbjct: 121 IGIKLIILLVNNWDDYGGKKQYVDWARSKGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRV 180

Query: 181 NTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEG 240
           NT + + Y D+P IM W+LMNEPRC VD SG T+  W+ EM  +VKS+D  HL+  G EG
Sbjct: 181 NTFTKVAYKDEPAIMAWQLMNEPRCGVDKSGKTLMDWINEMAPFVKSVDPNHLLSTGHEG 240

Query: 241 FYGDSNPSR-IKFNP-NGYKVGTDFVTNNLVKVIDFATIHAYPDSWLPGQSEATKMAFLE 300
           FYGDS+P R    NP +   VG DF+ N+ +  IDFA++H   D W     + +++AF++
Sbjct: 241 FYGDSSPERKNSLNPVSANTVGADFIANHNIDAIDFASMHCGSDLWFQRLDQNSRLAFIK 300

Query: 301 EWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGT 360
            W++ H  D++ ILKKP+I AEFG      +    +++ +RD   T  Y  IY  A+ G 
Sbjct: 301 RWLEGHIEDAQNILKKPVILAEFGLG----SDTPRYTLANRDGVFTTTYDIIYASAQKGG 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPSTTAIITQQSKNMAAL 396
           + AG L W+V++EGM ++     I+LS   ST  II++ ++ M  L
Sbjct: 361 SAAGALFWEVISEGMSNFAGPSSIILSDKSSTVNIISEHARKMGLL 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141751.12.7e-23297.04mannan endo-1,4-beta-mannosidase 5-like [Momordica charantia][more]
XP_038890634.11.4e-18579.16mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida][more]
XP_008459570.11.8e-18377.23PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 ma... [more]
XP_004141536.24.4e-18276.67mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical pr... [more]
XP_004141631.35.5e-17774.94mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo... [more]
Match NameE-valueIdentityDescription
Q9FZ038.9e-13860.48Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 ... [more]
Q6YM508.9e-13860.48Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 ... [more]
O485401.7e-13358.58Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 ... [more]
Q9FUQ61.2e-12955.56Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 ... [more]
Q0JKM91.1e-11652.79Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Match NameE-valueIdentityDescription
A0A6J1CK631.3e-23297.04Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 ... [more]
A0A5D3BKZ38.6e-18477.23Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CB048.6e-18477.23Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV... [more]
A0A0A0KSS22.1e-18276.67Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3... [more]
A0A5A7TCI22.8e-16672.73Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT5G66460.18.7e-11249.73Glycosyl hydrolase superfamily protein [more]
AT3G10890.13.9e-10445.10Glycosyl hydrolase superfamily protein [more]
AT5G01930.11.6e-10249.05Glycosyl hydrolase superfamily protein [more]
AT1G02310.13.3e-9546.74Glycosyl hydrolase superfamily protein [more]
AT3G10900.12.1e-9442.61Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..396
e-value: 3.8E-119
score: 400.0
NoneNo IPR availablePANTHERPTHR31451:SF53MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEINcoord: 2..397
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 40..322
e-value: 4.8E-13
score: 49.2
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 2..397
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS028761.1MS028761.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds