MS028657 (gene) Bitter gourd (TR) v1

Overview
NameMS028657
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPatatin
Locationscaffold378: 662883 .. 665467 (-)
RNA-Seq ExpressionMS028657
SyntenyMS028657
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACTGATTTTGGAAAGGGAAATATGGTAACAATTTTGAGTGTTGATGGAGGCGGCATTAGAGGCATCATTCCCGGAACTATTCTTGCTTTTTCTGGAGTCCAAGCTTCAGGTAATAAACATAATATATGCTCCAAACGTCAAATATGTCATAACCAATTTAAGATTTTAAGTCCAGCGATAGTTTTATGTGATATTAGAACGAATAATGGGTTAATGTGTATTTAATATATTTTTTGGTGATTTGGTTTGGGTTAGGAATTGGACGGTCCAGATGTGAGAATAACATATTACTTCGACATTGCAGGAACAAACACGGGTGGTTTGGTTAGCACCATGCTTTCAGCTCCCGATAAAAACAATCGGCCTTTATACGTTGCAAAAGACATCACTCCCTTCTATTTAGAACACGGTCCCAAAATCTTCCCACAAAGAAAGTTAGTATCTCTTTTTCCATAATTTCCAACACATTCGTTATGGTTTCATTTACTAAACTAGTGTTGCATATGGCAGCCATATGTTATAAGTTCCATGGTGAACTTGTTTGGCCAAGTCACGGGGCCAAAGTATGATGACAAATACTTGAAGTCGTTGATAAATGGAATACTTGGAGACCTAATTGTTAGGCAAACACTCACCCATATTGTTATTCCCACGTTGGACATTAAGCGTCTTCAACATATCATCTTCACCACAACTGATGTATGGCAAAACTAGTAGTTAACTATGTTTTCCCTAGCTCATATATCACTGATATATACCTAATATAAAACACATCACTGATATAAAGATTCTTACATTTATGCTAGGCGAAATGGAACGAGTTGAAGAATCCAAAGTTGGCTGATGTATGCATTAGTACCTCTGCAGCACCAACTTTCTTACCAAGCCATGAGTTTCAAACTAAGGATAACAATGGAAATACTCAAACTTTTGATATGGTTGATGGTGGAGTTGCTGCCAACAATGCAGTAAGCTCCTCTCCTCTTTAGTATTTTTTTTTTTTTTTGGAATAAAAATATATTACAAAGACTATCAAAGATCACTAAAATTGTTACCGGAACAAAAGCACACAAGTTATAATCATGGAATCGACTAGATCATCCGACTATCGATTATAAGTTCATAACCCTCATTCCCATTTTAGGCAGAACTTGTCAATTAATTCTTTGATTCGAGTTAGTAAGAGAGATCTTGGACTAAGAAATAGATTCTATCAACTAAACCAAAATAGGATATCATAAGAAATTTCAATAATTGAGCTCATTGATATTCCTGGTATAGTAGATCCTACCACACATACAATCAACTCAATTCGATGAAATTATATTTGATCAGCTGACTAGAATTTTTAACCCGTTATATTTTTATGACCTTGTTACACAATCAATAATATTTTGATTGAAATTGATGTGTAGGCTTTAGCTGCCATAACCCATGTGACTAAAAGAGATCACTACCATGAGACAAAAAAGTGAATTTTTGAAGATGAAACCCATGGAAACAAAAAAGATGTTGGTCCTTTCTTTAGGCACTGGTGCAGCCAAGAATGATGGCAAATATAGTGCTGCTAAGTCTGCCAAATGGGGTATCTTAATGGGGTCTACGACGCTGGAGCAACACAAATAGTTGACATTTTAGTGATGCAAGTGCTCATATGGTCGACTATCATATTTCTACCATCTTCCAATCTTCGCATTGTAAAAAAAAAAACTATCTTCGTATTCAGGTATATATTTATAATTAAGTTTGTTTCCTAATTGTAATTTCTCTAGTTAAGAATACTTTTTGATTTCTTACCAAAATTTTAAGATCTTTTTTAGACTCACTTTTAGTTTTTATTTTTCCTAAGTGAAATTTCTCTGGTTAAGAACACTTTTGATTTCTTACCAAAATTTCAAGATTATTTCAGACTCACTTTTAGTTTATATATATATATATACATACATACATGCACGTATATACCTAACTGTTAAATGGAAAGTTTATTCTAAACTTTTCAATGTTGTAACTAAGAAGTTTCTAAATCTTCAATTTTGTATCTAATAAATACTTCAGGTTTGATATTTTTTAAAATTTGCTAATCACTTATAATTCAAATAAAATTATGCCCTTTCAATTTTGTATTATGTTTAATAGGTCCACAAATTACAAAATGAAACCTATCAGACTCATAAAAAGCTGACGAATTAGAGACTTTTTAAAGTTTAAAGACATATTAGTTATGAAAATTTAAGTACTGAACATATAATTAAACTTAATCTTTTTGATCTTTTGGACAGAATTTTTTTCAATTGTTACTATAAATACAATCAATTAAACATAATCTAATATAAGATATGATTGTGGTCGCACAGGATGACACATTAACTGGTGATGTTTCGTCTGTCGATACTGCGACCAAAGAGAATTTACAGAAGCTTGTAGAGGTCGGAGAGAAATTGCTGAAGAAACCATTATCGAGGGTAAATTTGGAATCTGGAAAGTTTGAGCCAGTCGATGGAGAAGGTACCAATGAAGATGCCTTTACTGAATTTGCTAAAATGCTATTGGAAGAGAGGAAGTTACGACTAAGCCCTTGA

mRNA sequence

ATGGCAACTGATTTTGGAAAGGGAAATATGGTAACAATTTTGAGTGTTGATGGAGGCGGCATTAGAGGCATCATTCCCGGAACTATTCTTGCTTTTTCTGGAGTCCAAGCTTCAGTCACGGGGCCAAAGTATGATGACAAATACTTGAAGTCGTTGATAAATGGAATACTTGGAGACCTAATTGTTAGGCAAACACTCACCCATATTGTTATTCCCACGTTGGACATTAAGCGTCTTCAACATATCATCTTCACCACAACTGATGCGAAATGGAACGAGTTGAAGAATCCAAAGTTGGCTGATGTATGCATTAGTACCTCTGCAGCACCAACTTTCTTACCAAGCCATGAGTTTCAAACTAAGGATAACAATGGAAATACTCAAACTTTTGATATGGTTGATGGTGGAGTTGCTGCCAACAATGCAGATGACACATTAACTGGTGATGTTTCGTCTGTCGATACTGCGACCAAAGAGAATTTACAGAAGCTTGTAGAGGTCGGAGAGAAATTGCTGAAGAAACCATTATCGAGGGTAAATTTGGAATCTGGAAAGTTTGAGCCAGTCGATGGAGAAGGTACCAATGAAGATGCCTTTACTGAATTTGCTAAAATGCTATTGGAAGAGAGGAAGTTACGACTAAGCCCTTGA

Coding sequence (CDS)

ATGGCAACTGATTTTGGAAAGGGAAATATGGTAACAATTTTGAGTGTTGATGGAGGCGGCATTAGAGGCATCATTCCCGGAACTATTCTTGCTTTTTCTGGAGTCCAAGCTTCAGTCACGGGGCCAAAGTATGATGACAAATACTTGAAGTCGTTGATAAATGGAATACTTGGAGACCTAATTGTTAGGCAAACACTCACCCATATTGTTATTCCCACGTTGGACATTAAGCGTCTTCAACATATCATCTTCACCACAACTGATGCGAAATGGAACGAGTTGAAGAATCCAAAGTTGGCTGATGTATGCATTAGTACCTCTGCAGCACCAACTTTCTTACCAAGCCATGAGTTTCAAACTAAGGATAACAATGGAAATACTCAAACTTTTGATATGGTTGATGGTGGAGTTGCTGCCAACAATGCAGATGACACATTAACTGGTGATGTTTCGTCTGTCGATACTGCGACCAAAGAGAATTTACAGAAGCTTGTAGAGGTCGGAGAGAAATTGCTGAAGAAACCATTATCGAGGGTAAATTTGGAATCTGGAAAGTTTGAGCCAGTCGATGGAGAAGGTACCAATGAAGATGCCTTTACTGAATTTGCTAAAATGCTATTGGAAGAGAGGAAGTTACGACTAAGCCCTTGA

Protein sequence

MATDFGKGNMVTILSVDGGGIRGIIPGTILAFSGVQASVTGPKYDDKYLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANNADDTLTGDVSSVDTATKENLQKLVEVGEKLLKKPLSRVNLESGKFEPVDGEGTNEDAFTEFAKMLLEERKLRLSP
Homology
BLAST of MS028657 vs. NCBI nr
Match: XP_016902153.1 (PREDICTED: patatin-like protein 2 isoform X2 [Cucumis melo])

HSP 1 Score: 275.4 bits (703), Expect = 4.2e-70
Identity = 157/282 (55.67%), Postives = 178/282 (63.12%), Query Frame = 0

Query: 7   KGNMVTILSVDGGGIRGIIPGTILAF---------------------------------- 66
           KG  +TILS+DGGGIRGIIPGTILAF                                  
Sbjct: 8   KGKRITILSIDGGGIRGIIPGTILAFLESKLQELDGPDVRIADYFDVITGTSTGGLVTSM 67

Query: 67  ------------------------------------SGVQAS---VTGPKYDDKYLKSLI 126
                                               S V  S   V GP+YD KYL++LI
Sbjct: 68  LTAPDKNNRPLYAAKDLTRFYVEHAPKIFPQRNHFLSSVMNSLGKVMGPRYDGKYLRTLI 127

Query: 127 NGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFL 186
           N +LGD+ +++TLT ++IP  DIKRLQ +IFTT DAK +ELKNPKLADVCISTSAAPT L
Sbjct: 128 NNLLGDITLKETLTQVIIPAFDIKRLQPVIFTTVDAKLDELKNPKLADVCISTSAAPTIL 187

Query: 187 PSHEFQTKDNNGNTQTFDMVDGGVAANNADDTLTGDVSSVDTATKENLQKLVEVGEKLLK 216
           PSHEF+ KD+ GN + FDMVDG VAANN DDTLTGDVSSVD AT+ENL KL+EVGE LLK
Sbjct: 188 PSHEFEIKDSKGNKRRFDMVDGAVAANNPDDTLTGDVSSVDVATEENLLKLIEVGENLLK 247

BLAST of MS028657 vs. NCBI nr
Match: XP_022155458.1 (patatin-like protein 3 [Momordica charantia])

HSP 1 Score: 264.6 bits (675), Expect = 7.4e-67
Identity = 174/392 (44.39%), Postives = 189/392 (48.21%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF---------------------------- 60
           MA DF KG MVTILSVDGGGIRGIIPGTILAF                            
Sbjct: 1   MAADFAKGKMVTILSVDGGGIRGIIPGTILAFLESKLQELDGPDVRIADYFDVIAGTSTG 60

Query: 61  ---------------------------------------------SGVQASVTGPKYDDK 120
                                                        + +   V GPKYD K
Sbjct: 61  GLVTSMLSAPDKNNRPLYAAKDLTHFYLEHAPKIFPQRNHLLSSVANLFGQVMGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLING+LGDL ++QTLT +VIP  DIK LQ +IFTT DAKWNELKNPKLADVCISTS
Sbjct: 121 YLRSLINGLLGDLTLKQTLTQVVIPAFDIKLLQPVIFTTIDAKWNELKNPKLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPT+LP HEFQTKD+ GNT+ FDMVDGGVAANN                          
Sbjct: 181 AAPTYLPGHEFQTKDSKGNTRNFDMVDGGVAANNPTLAAMTHVTKEISILRRRMDLLKIK 240

BLAST of MS028657 vs. NCBI nr
Match: XP_022158233.1 (patatin-like protein 2 [Momordica charantia])

HSP 1 Score: 260.4 bits (664), Expect = 1.4e-65
Identity = 173/392 (44.13%), Postives = 189/392 (48.21%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF--SGVQ---------------------- 60
           MA DFGKG MVTILS+DGGGIRGIIPGTILAF  S +Q                      
Sbjct: 1   MAADFGKGKMVTILSIDGGGIRGIIPGTILAFLESKLQELDGPDVRIVDYFDVMAGTSTG 60

Query: 61  -------------------------------------------------ASVTGPKYDDK 120
                                                              V GPKYD K
Sbjct: 61  ALVTSMLSAPNENNRPLYAAKDLTKFYLEHAPKIFPQRNHLLSSVVNLFGKVMGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLIN +LGDL ++QTLTH+VIPT DIK LQ +IF T +A+W EL+NP+LADVCISTS
Sbjct: 121 YLRSLINELLGDLTIKQTLTHVVIPTFDIKLLQPVIFNTIEARWKELQNPRLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPTF P HEFQTKD+ GNT+ FDMVDGGVAANN                          
Sbjct: 181 AAPTFFPGHEFQTKDSRGNTRNFDMVDGGVAANNPTLAAITHVTKEISLLRRTMEFMKIK 240

BLAST of MS028657 vs. NCBI nr
Match: XP_023007101.1 (patatin-like protein 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 255.8 bits (652), Expect = 3.4e-64
Identity = 167/392 (42.60%), Postives = 188/392 (47.96%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF---------------------------S 60
           MA DFGKG ++TILSVDGGGIRGIIPGTILAF                            
Sbjct: 1   MAPDFGKGKVITILSVDGGGIRGIIPGTILAFLESKLQELDGPDARIADYFDVIAGTSTG 60

Query: 61  GVQAS----------------------------------------------VTGPKYDDK 120
           G+  S                                              V GPKYD K
Sbjct: 61  GLVTSMLTAPNKDNRPLYAAKDLTRFYMEHAPKIFPQRSHFLSSVVNFLGRVIGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLI+ +LGD  ++QTLT ++IP  DIKRLQ +IFTT DAKW+ELKNPKLADVCISTS
Sbjct: 121 YLRSLIHELLGDTTLKQTLTQVIIPAFDIKRLQPVIFTTIDAKWDELKNPKLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPTFLP HEFQT+D+ GN + FDMVDGGVAANN                          
Sbjct: 181 AAPTFLPGHEFQTQDSKGNIRKFDMVDGGVAANNPTLAAITHVTKEMSLLWHRNELLNIK 240

BLAST of MS028657 vs. NCBI nr
Match: XP_038902677.1 (patatin-like protein 3 [Benincasa hispida])

HSP 1 Score: 255.4 bits (651), Expect = 4.5e-64
Identity = 165/393 (41.98%), Postives = 187/393 (47.58%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF---------------------------- 60
           MA D  KG  +TILS+DGGGIRGIIPGTILAF                            
Sbjct: 1   MAADCEKGKRITILSIDGGGIRGIIPGTILAFLESKLQELDGPDVRIADYFDVIAGTSTG 60

Query: 61  ---------------------------------------------SGVQASVTGPKYDDK 120
                                                          +   V GPKYD K
Sbjct: 61  GLITSMLTAPDKNNRPLYAAKDLSRFYIEHAPKIFPQRNHFLSSTMNLFGKVMGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLI G+LGD+ +++TLT ++IP  DIKRLQ +IFTT DAKW+ELKNPKLADVCISTS
Sbjct: 121 YLRSLIKGLLGDITLKETLTQVIIPAFDIKRLQPVIFTTADAKWDELKNPKLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPT LPSHEFQTKD+ GN + FDMVDGGVAANN                          
Sbjct: 181 AAPTLLPSHEFQTKDSKGNIRKFDMVDGGVAANNPTLAAMTHVRKEMSIWKERSELMAIK 240

BLAST of MS028657 vs. ExPASy Swiss-Prot
Match: O23181 (Patatin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLP3 PE=2 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 2.6e-38
Identity = 121/390 (31.03%), Postives = 158/390 (40.51%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF----------------------SGVQAS------- 67
           G +VTILS+DGGGIRGIIPGTILA+                      SG           
Sbjct: 32  GQLVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVISGTSTGGLIVAML 91

Query: 68  ----------------------------------------------------VTGPKYDD 127
                                                               V GPK++ 
Sbjct: 92  TAQDQSGGHSRNSNRPLFEAKEIVPFYLKHSPKIFPQPRGIFCGWGETIVRLVGGPKFNG 151

Query: 128 KYLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCIST 187
           KYL  L+ G LGD  + Q+LT++VIP  DIK+LQ +IF++  A  N+  N KL+D+CIST
Sbjct: 152 KYLHDLVEGFLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNQAMNAKLSDICIST 211

Query: 188 SAAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANNA------------------------ 216
           SAAPTF P+H F  +D+ G    F+++DGG+AANN                         
Sbjct: 212 SAAPTFFPAHRFTNEDSEGIKHEFNLIDGGIAANNPTLCAIAEVTKQIIKKNPVMGDISP 271

BLAST of MS028657 vs. ExPASy Swiss-Prot
Match: A2YW91 (Patatin-like protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=PLP2 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.1e-36
Identity = 119/383 (31.07%), Postives = 152/383 (39.69%), Query Frame = 0

Query: 7   KGNMVTILSVDGGGIRGIIPGTILAF---------------------------------- 66
           K  MVT+LS+DGGG+RGIIP TILAF                                  
Sbjct: 17  KVKMVTVLSIDGGGVRGIIPATILAFLEKELQKLDGPDARIADYFDVVAGTSTGGLLTAM 76

Query: 67  ---------------------------------------SGVQASVTGPKYDDKYLKSLI 126
                                                  +G    V+GPKYD KYL SL+
Sbjct: 77  LTAPNENNRPLFAADELAKFYIEHSPSIFPQKNWVLSKIAGTLRMVSGPKYDGKYLHSLL 136

Query: 127 NGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFL 186
              LGD  + + LT++VIPT DI  LQ  IF+  + K+  LKN  L+D+ ISTSAAPTF 
Sbjct: 137 REKLGDTRLDKALTNVVIPTFDIANLQPTIFSKFELKYKPLKNALLSDISISTSAAPTFF 196

Query: 187 PSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------------- 214
           P+H F+TKD+NG T+ F++VDGGVAANN                                
Sbjct: 197 PAHYFETKDDNGQTREFNLVDGGVAANNPTLCAMSQVSKYIILEDKEDCDFFPVKPTEYG 256

BLAST of MS028657 vs. ExPASy Swiss-Prot
Match: Q6ZJD3 (Patatin-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP2 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.1e-36
Identity = 119/383 (31.07%), Postives = 152/383 (39.69%), Query Frame = 0

Query: 7   KGNMVTILSVDGGGIRGIIPGTILAF---------------------------------- 66
           K  MVT+LS+DGGG+RGIIP TILAF                                  
Sbjct: 17  KVKMVTVLSIDGGGVRGIIPATILAFLEKELQKLDGPDARIADYFDVVAGTSTGGLLTAM 76

Query: 67  ---------------------------------------SGVQASVTGPKYDDKYLKSLI 126
                                                  +G    V+GPKYD KYL SL+
Sbjct: 77  LTAPNENNRPLFAADELAKFYIEHSPSIFPQKNWVLSKIAGTLRMVSGPKYDGKYLHSLL 136

Query: 127 NGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFL 186
              LGD  + + LT++VIPT DI  LQ  IF+  + K+  LKN  L+D+ ISTSAAPTF 
Sbjct: 137 REKLGDTRLDKALTNVVIPTFDIANLQPTIFSKFELKYKPLKNALLSDISISTSAAPTFF 196

Query: 187 PSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------------- 214
           P+H F+TKD+NG T+ F++VDGGVAANN                                
Sbjct: 197 PAHYFETKDDNGQTREFNLVDGGVAANNPTLCAMSQVSKYIILEDKEDCDFFPVKPTEYG 256

BLAST of MS028657 vs. ExPASy Swiss-Prot
Match: B8AQW7 (Patatin-like protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=PLP1 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 9.1e-36
Identity = 114/380 (30.00%), Postives = 158/380 (41.58%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF----------------------------------- 67
           G  VT+L++DGGGIRG+IPGTILAF                                   
Sbjct: 14  GQRVTLLAIDGGGIRGLIPGTILAFLEARLQELDGPDARLADYFDCIAGTSTGGLITAML 73

Query: 68  -----------------------------------SGVQASVTGPKYDDKYLKSLINGIL 127
                                              +   A++T P+Y+ KYL+  I  +L
Sbjct: 74  AAPGDHGRPLFAASDINRFYLDNGPRIFPQKRCGMAAAMAALTRPRYNGKYLQGKIRKML 133

Query: 128 GDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFLPSHE 187
           G+  VR TLT++VIPT D++ LQ  IF+T DAK   LKN  L+D+CISTSAAPT+LP+H 
Sbjct: 134 GETRVRDTLTNVVIPTFDVRLLQPTIFSTYDAKSMPLKNALLSDICISTSAAPTYLPAHC 193

Query: 188 FQTKDN-NGNTQTFDMVDGGVAANN----------------------------------- 215
           FQT D+  G  + FD++DGGVAANN                                   
Sbjct: 194 FQTTDDATGKVREFDLIDGGVAANNPTMVAMTQITKKIMVKDKEELYPVKPSDCGKFLVL 253

BLAST of MS028657 vs. ExPASy Swiss-Prot
Match: Q84QY3 (Patatin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP1 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 9.1e-36
Identity = 114/380 (30.00%), Postives = 158/380 (41.58%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF----------------------------------- 67
           G  VT+L++DGGGIRG+IPGTILAF                                   
Sbjct: 32  GQRVTLLAIDGGGIRGLIPGTILAFLEARLQELDGPDARLADYFDCIAGTSTGGLITAML 91

Query: 68  -----------------------------------SGVQASVTGPKYDDKYLKSLINGIL 127
                                              +   A++T P+Y+ KYL+  I  +L
Sbjct: 92  AAPGDHGRPLFAASDINRFYLDNGPLIFPQKRCGMAAAMAALTRPRYNGKYLQGKIRKML 151

Query: 128 GDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFLPSHE 187
           G+  VR TLT++VIPT D++ LQ  IF+T DAK   LKN  L+D+CISTSAAPT+LP+H 
Sbjct: 152 GETRVRDTLTNVVIPTFDVRLLQPTIFSTYDAKSMPLKNALLSDICISTSAAPTYLPAHC 211

Query: 188 FQTKDN-NGNTQTFDMVDGGVAANN----------------------------------- 215
           FQT D+  G  + FD++DGGVAANN                                   
Sbjct: 212 FQTTDDATGKVREFDLIDGGVAANNPTMVAMTQITKKIMVKDKEELYPVKPSDCGKFLVL 271

BLAST of MS028657 vs. ExPASy TrEMBL
Match: A0A1S4E1Q3 (Patatin OS=Cucumis melo OX=3656 GN=LOC103497452 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.0e-70
Identity = 157/282 (55.67%), Postives = 178/282 (63.12%), Query Frame = 0

Query: 7   KGNMVTILSVDGGGIRGIIPGTILAF---------------------------------- 66
           KG  +TILS+DGGGIRGIIPGTILAF                                  
Sbjct: 8   KGKRITILSIDGGGIRGIIPGTILAFLESKLQELDGPDVRIADYFDVITGTSTGGLVTSM 67

Query: 67  ------------------------------------SGVQAS---VTGPKYDDKYLKSLI 126
                                               S V  S   V GP+YD KYL++LI
Sbjct: 68  LTAPDKNNRPLYAAKDLTRFYVEHAPKIFPQRNHFLSSVMNSLGKVMGPRYDGKYLRTLI 127

Query: 127 NGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFL 186
           N +LGD+ +++TLT ++IP  DIKRLQ +IFTT DAK +ELKNPKLADVCISTSAAPT L
Sbjct: 128 NNLLGDITLKETLTQVIIPAFDIKRLQPVIFTTVDAKLDELKNPKLADVCISTSAAPTIL 187

Query: 187 PSHEFQTKDNNGNTQTFDMVDGGVAANNADDTLTGDVSSVDTATKENLQKLVEVGEKLLK 216
           PSHEF+ KD+ GN + FDMVDG VAANN DDTLTGDVSSVD AT+ENL KL+EVGE LLK
Sbjct: 188 PSHEFEIKDSKGNKRRFDMVDGAVAANNPDDTLTGDVSSVDVATEENLLKLIEVGENLLK 247

BLAST of MS028657 vs. ExPASy TrEMBL
Match: A0A0A0LJL8 (Patatin OS=Cucumis sativus OX=3659 GN=Csa_2G292850 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 7.2e-68
Identity = 160/321 (49.84%), Postives = 183/321 (57.01%), Query Frame = 0

Query: 5   FGKGNMVTILSVDGGGIRGIIPGTILAF--SGVQ----------------------ASVT 64
           F KG  +TILS+DGGGIRGIIPGTILAF  S +Q                        V 
Sbjct: 8   FEKGKKITILSIDGGGIRGIIPGTILAFLESKLQYHSSILKLFTNFYFLSSVVNMFGKVM 67

Query: 65  GPKYDDKYLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLA 124
           GPKYD KYL+SLIN +LGD+ +++TLT ++IP  DIK LQ +IF+T DAKW+ LKNPKLA
Sbjct: 68  GPKYDGKYLRSLINRLLGDITLKETLTQVIIPAFDIKLLQPVIFSTLDAKWDALKNPKLA 127

Query: 125 DVCISTSAAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN------------------- 184
           DVCISTSAAPTFLP HEFQTKD+ GNT+ FDMVDGGVAANN                   
Sbjct: 128 DVCISTSAAPTFLPGHEFQTKDSKGNTRNFDMVDGGVAANNPTLAALTHVTKEMSILRKR 187

Query: 185 ------------------------------------------------------------ 217
                                                                       
Sbjct: 188 SELLKIKPMESKRMLILSLGTGVAKNDEKGATPIVDIFSDASADMVDYHISSVFQSEHND 247

BLAST of MS028657 vs. ExPASy TrEMBL
Match: A0A6J1DN05 (Patatin OS=Momordica charantia OX=3673 GN=LOC111022597 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.6e-67
Identity = 174/392 (44.39%), Postives = 189/392 (48.21%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF---------------------------- 60
           MA DF KG MVTILSVDGGGIRGIIPGTILAF                            
Sbjct: 1   MAADFAKGKMVTILSVDGGGIRGIIPGTILAFLESKLQELDGPDVRIADYFDVIAGTSTG 60

Query: 61  ---------------------------------------------SGVQASVTGPKYDDK 120
                                                        + +   V GPKYD K
Sbjct: 61  GLVTSMLSAPDKNNRPLYAAKDLTHFYLEHAPKIFPQRNHLLSSVANLFGQVMGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLING+LGDL ++QTLT +VIP  DIK LQ +IFTT DAKWNELKNPKLADVCISTS
Sbjct: 121 YLRSLINGLLGDLTLKQTLTQVVIPAFDIKLLQPVIFTTIDAKWNELKNPKLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPT+LP HEFQTKD+ GNT+ FDMVDGGVAANN                          
Sbjct: 181 AAPTYLPGHEFQTKDSKGNTRNFDMVDGGVAANNPTLAAMTHVTKEISILRRRMDLLKIK 240

BLAST of MS028657 vs. ExPASy TrEMBL
Match: A0A6J1DVI5 (Patatin OS=Momordica charantia OX=3673 GN=LOC111024765 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 6.7e-66
Identity = 173/392 (44.13%), Postives = 189/392 (48.21%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF--SGVQ---------------------- 60
           MA DFGKG MVTILS+DGGGIRGIIPGTILAF  S +Q                      
Sbjct: 1   MAADFGKGKMVTILSIDGGGIRGIIPGTILAFLESKLQELDGPDVRIVDYFDVMAGTSTG 60

Query: 61  -------------------------------------------------ASVTGPKYDDK 120
                                                              V GPKYD K
Sbjct: 61  ALVTSMLSAPNENNRPLYAAKDLTKFYLEHAPKIFPQRNHLLSSVVNLFGKVMGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLIN +LGDL ++QTLTH+VIPT DIK LQ +IF T +A+W EL+NP+LADVCISTS
Sbjct: 121 YLRSLINELLGDLTIKQTLTHVVIPTFDIKLLQPVIFNTIEARWKELQNPRLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPTF P HEFQTKD+ GNT+ FDMVDGGVAANN                          
Sbjct: 181 AAPTFFPGHEFQTKDSRGNTRNFDMVDGGVAANNPTLAAITHVTKEISLLRRTMEFMKIK 240

BLAST of MS028657 vs. ExPASy TrEMBL
Match: A0A6J1KZK6 (Patatin OS=Cucurbita maxima OX=3661 GN=LOC111499702 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.7e-64
Identity = 167/392 (42.60%), Postives = 188/392 (47.96%), Query Frame = 0

Query: 1   MATDFGKGNMVTILSVDGGGIRGIIPGTILAF---------------------------S 60
           MA DFGKG ++TILSVDGGGIRGIIPGTILAF                            
Sbjct: 1   MAPDFGKGKVITILSVDGGGIRGIIPGTILAFLESKLQELDGPDARIADYFDVIAGTSTG 60

Query: 61  GVQAS----------------------------------------------VTGPKYDDK 120
           G+  S                                              V GPKYD K
Sbjct: 61  GLVTSMLTAPNKDNRPLYAAKDLTRFYMEHAPKIFPQRSHFLSSVVNFLGRVIGPKYDGK 120

Query: 121 YLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTS 180
           YL+SLI+ +LGD  ++QTLT ++IP  DIKRLQ +IFTT DAKW+ELKNPKLADVCISTS
Sbjct: 121 YLRSLIHELLGDTTLKQTLTQVIIPAFDIKRLQPVIFTTIDAKWDELKNPKLADVCISTS 180

Query: 181 AAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANN-------------------------- 217
           AAPTFLP HEFQT+D+ GN + FDMVDGGVAANN                          
Sbjct: 181 AAPTFLPGHEFQTQDSKGNIRKFDMVDGGVAANNPTLAAITHVTKEMSLLWHRNELLNIK 240

BLAST of MS028657 vs. TAIR 10
Match: AT4G37050.1 (PATATIN-like protein 4 )

HSP 1 Score: 160.2 bits (404), Expect = 1.8e-39
Identity = 121/390 (31.03%), Postives = 158/390 (40.51%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF----------------------SGVQAS------- 67
           G +VTILS+DGGGIRGIIPGTILA+                      SG           
Sbjct: 32  GQLVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVISGTSTGGLIVAML 91

Query: 68  ----------------------------------------------------VTGPKYDD 127
                                                               V GPK++ 
Sbjct: 92  TAQDQSGGHSRNSNRPLFEAKEIVPFYLKHSPKIFPQPRGIFCGWGETIVRLVGGPKFNG 151

Query: 128 KYLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCIST 187
           KYL  L+ G LGD  + Q+LT++VIP  DIK+LQ +IF++  A  N+  N KL+D+CIST
Sbjct: 152 KYLHDLVEGFLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNQAMNAKLSDICIST 211

Query: 188 SAAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANNA------------------------ 216
           SAAPTF P+H F  +D+ G    F+++DGG+AANN                         
Sbjct: 212 SAAPTFFPAHRFTNEDSEGIKHEFNLIDGGIAANNPTLCAIAEVTKQIIKKNPVMGDISP 271

BLAST of MS028657 vs. TAIR 10
Match: AT2G26560.1 (phospholipase A 2A )

HSP 1 Score: 148.7 bits (374), Expect = 5.5e-36
Identity = 120/380 (31.58%), Postives = 156/380 (41.05%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF--------SGVQA---------------------- 67
           GN+VTILS+DGGGIRG+IP  IL F         G +A                      
Sbjct: 16  GNLVTILSIDGGGIRGLIPAVILGFLESELQKLDGEEARLADYFDVIAGTSTGGLVTAML 75

Query: 68  -------------------------------------------SVTGPKYDDKYLKSLIN 127
                                                      S+TGPKYD KYL  LI+
Sbjct: 76  TAPNKEGRPLFAASEIKDFYLEQCPKIFPQDHFPFSAAKKLVKSLTGPKYDGKYLHQLIH 135

Query: 128 GILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFLP 187
             LGD  + QTLT++VIPT DIK LQ  IF++ + K + LK+  LAD+ ISTSAAPT+LP
Sbjct: 136 AKLGDTKLSQTLTNVVIPTFDIKHLQPTIFSSYEVKNHPLKDATLADIAISTSAAPTYLP 195

Query: 188 SHEFQTKDNNGNTQTFDMVDGGVAANN--------------------------------- 214
           +H F+ +D NGN + ++++DGGVAANN                                 
Sbjct: 196 AHFFKVEDLNGNAKEYNLIDGGVAANNPALLAIGEVTNEISGGSSDFFPIRPNDYGRFLV 255

BLAST of MS028657 vs. TAIR 10
Match: AT4G37070.2 (Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-31
Identity = 107/380 (28.16%), Postives = 152/380 (40.00%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF----------------------------------- 67
           G++VTILS+DGGG+RGII G ILAF                                   
Sbjct: 16  GSLVTILSLDGGGVRGIIAGVILAFLEKQLQELDGEEARLADYFDVIAGTSTGGLVTAML 75

Query: 68  -------------------------------SGVQA-------SVTGPKYDDKYLKSLIN 127
                                          +GV A        ++GPKY  KYL++L++
Sbjct: 76  TVPDETGRPHFAAKDIVPFYLEHCPKIFPQPTGVLALLPKLPKLLSGPKYSGKYLRNLLS 135

Query: 128 GILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFLP 187
            +LG+  + QTLT+IVIPT DIK+LQ  IF++     +   + K++D+CI TSAAPTF P
Sbjct: 136 KLLGETRLHQTLTNIVIPTFDIKKLQPTIFSSYQLLVDPSLDVKVSDICIGTSAAPTFFP 195

Query: 188 SHEFQTKDNNGNTQTFDMVDGGVAANNA-------------------------------- 214
            H F  +D+ GN   F++VDG V ANN                                 
Sbjct: 196 PHYFSNEDSQGNKTEFNLVDGAVTANNPTLVAMTAVSKQIVKNNPDMGKLKPLGFDRFLV 255

BLAST of MS028657 vs. TAIR 10
Match: AT4G37060.1 (PATATIN-like protein 5 )

HSP 1 Score: 128.3 bits (321), Expect = 7.7e-30
Identity = 102/380 (26.84%), Postives = 149/380 (39.21%), Query Frame = 0

Query: 8   GNMVTILSVDGGGIRGIIPGTILAF----------------------------------- 67
           G +VTILS+DGGG+RGII G ILA+                                   
Sbjct: 16  GTLVTILSLDGGGVRGIIAGVILAYLEKQLQELDGEHVRVADYFDVIAGTSTGGLVTAML 75

Query: 68  -------------------------------SGVQA-------SVTGPKYDDKYLKSLIN 127
                                          +GV A        ++GPKY   YL++ + 
Sbjct: 76  TAPDENGRPRFAAKEIVPFYLEHCPKIFPQPTGVLALLPKLPKLLSGPKYSGNYLRTTLG 135

Query: 128 GILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPKLADVCISTSAAPTFLP 187
            +LG+  +RQTLT++VIPT DIK LQ  IF++  A  +   + K++D+CI TSAAPT+ P
Sbjct: 136 KLLGETKLRQTLTNVVIPTFDIKTLQPTIFSSYQALTDPSLDVKVSDICIGTSAAPTYFP 195

Query: 188 SHEFQTKDNNGNTQTFDMVDGGVAANNA-------------------------------- 214
            + F  +D+ G T+ F++VDGGV ANN                                 
Sbjct: 196 PYYFSNEDSQGKTRHFNLVDGGVTANNPTLVAMTAVTKQIVNNNPDMGTLNPLGYDQFLV 255

BLAST of MS028657 vs. TAIR 10
Match: AT4G37060.2 (PATATIN-like protein 5 )

HSP 1 Score: 124.8 bits (312), Expect = 8.5e-29
Identity = 81/276 (29.35%), Postives = 123/276 (44.57%), Query Frame = 0

Query: 39  VTGPKYDDKYLKSLINGILGDLIVRQTLTHIVIPTLDIKRLQHIIFTTTDAKWNELKNPK 98
           ++GPKY   YL++ +  +LG+  +RQTLT++VIPT DIK LQ  IF++  A  +   + K
Sbjct: 141 LSGPKYSGNYLRTTLGKLLGETKLRQTLTNVVIPTFDIKTLQPTIFSSYQALTDPSLDVK 200

Query: 99  LADVCISTSAAPTFLPSHEFQTKDNNGNTQTFDMVDGGVAANNA---------------- 158
           ++D+CI TSAAPT+ P + F  +D+ G T+ F++VDGGV ANN                 
Sbjct: 201 VSDICIGTSAAPTYFPPYYFSNEDSQGKTRHFNLVDGGVTANNPTLVAMTAVTKQIVNNN 260

Query: 159 ------------------------------------------------------------ 214
                                                                       
Sbjct: 261 PDMGTLNPLGYDQFLVISIGTGSAKKEERYSAKKAAKWGIISWLYEDGTTPILDITFESS 320


HSP 2 Score: 41.2 bits (95), Expect = 1.2e-03
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 0

Query: 8  GNMVTILSVDGGGIRGIIPGTILAFSGVQASV 40
          G +VTILS+DGGG+RGII G ILA+   Q  V
Sbjct: 6  GTLVTILSLDGGGVRGIIAGVILAYLEKQLQV 37

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016902153.14.2e-7055.67PREDICTED: patatin-like protein 2 isoform X2 [Cucumis melo][more]
XP_022155458.17.4e-6744.39patatin-like protein 3 [Momordica charantia][more]
XP_022158233.11.4e-6544.13patatin-like protein 2 [Momordica charantia][more]
XP_023007101.13.4e-6442.60patatin-like protein 2 isoform X1 [Cucurbita maxima][more]
XP_038902677.14.5e-6441.98patatin-like protein 3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O231812.6e-3831.03Patatin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PLP3 PE=2 SV=2[more]
A2YW913.1e-3631.07Patatin-like protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=PLP2 PE=3 SV=1[more]
Q6ZJD33.1e-3631.07Patatin-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP2 PE=3 SV=... [more]
B8AQW79.1e-3630.00Patatin-like protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=PLP1 PE=3 SV=1[more]
Q84QY39.1e-3630.00Patatin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLP1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S4E1Q32.0e-7055.67Patatin OS=Cucumis melo OX=3656 GN=LOC103497452 PE=3 SV=1[more]
A0A0A0LJL87.2e-6849.84Patatin OS=Cucumis sativus OX=3659 GN=Csa_2G292850 PE=3 SV=1[more]
A0A6J1DN053.6e-6744.39Patatin OS=Momordica charantia OX=3673 GN=LOC111022597 PE=3 SV=1[more]
A0A6J1DVI56.7e-6644.13Patatin OS=Momordica charantia OX=3673 GN=LOC111024765 PE=3 SV=1[more]
A0A6J1KZK61.7e-6442.60Patatin OS=Cucurbita maxima OX=3661 GN=LOC111499702 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37050.11.8e-3931.03PATATIN-like protein 4 [more]
AT2G26560.15.5e-3631.58phospholipase A 2A [more]
AT4G37070.21.1e-3128.16Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein [more]
AT4G37060.17.7e-3026.84PATATIN-like protein 5 [more]
AT4G37060.28.5e-2929.35PATATIN-like protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002641Patatin-like phospholipase domainPFAMPF01734Patatincoord: 40..141
e-value: 2.4E-6
score: 27.9
IPR002641Patatin-like phospholipase domainPROSITEPS51635PNPLAcoord: 1..147
score: 14.182209
NoneNo IPR availableGENE3D3.40.1090.10Cytosolic phospholipase A2 catalytic domaincoord: 31..216
e-value: 8.6E-40
score: 139.1
NoneNo IPR availablePANTHERPTHR32176XYLOSE ISOMERASEcoord: 143..214
NoneNo IPR availablePANTHERPTHR32176:SF49PATATINcoord: 143..214
NoneNo IPR availablePANTHERPTHR32176:SF49PATATINcoord: 7..32
coord: 38..141
NoneNo IPR availablePANTHERPTHR32176XYLOSE ISOMERASEcoord: 7..32
coord: 38..141
IPR016035Acyl transferase/acyl hydrolase/lysophospholipaseSUPERFAMILY52151FabD/lysophospholipase-likecoord: 9..182

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS028657.1MS028657.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process